"
Team:Austin Texas/Week of July 23 to July 29
From 2012.igem.org
(Difference between revisions)
(→Design of Converter Plasmid) |
PeterOtoupal (Talk | contribs) (→CBB5 Caffeine Inducible Promoters) |
||
| Line 30: | Line 30: | ||
== CBB5 Caffeine Inducible Promoters == | == CBB5 Caffeine Inducible Promoters == | ||
| + | *Peter | ||
| + | **7/23- Cotransformation of PRA301-ndmApro LacZ reporter plasmid (with spectinomycin reporter) and ORF1 -4,5,6 plasmid minipreps. Ran out of PRA301-ndmApro plasmid, so only ORF1-4,5 were cotransformed. ORF1- 6 was transformed by itself as a control. Plated on kanamycin/XGal plates. | ||
| + | **7/24- Cotransformation failed. Likely due to too much plasmid, mixed with poor transformation. | ||
== Inducible Odor == | == Inducible Odor == | ||
Revision as of 21:26, 31 July 2012
Contents |
Notebook for week of July 23 to July 29
Design of Converter Plasmid
- Razan
- 7/24- Amplified pACYC backbone successfully with new primers for both control and converter plasmids.
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 7/23- Cotransformation of PRA301-ndmApro LacZ reporter plasmid (with spectinomycin reporter) and ORF1 -4,5,6 plasmid minipreps. Ran out of PRA301-ndmApro plasmid, so only ORF1-4,5 were cotransformed. ORF1- 6 was transformed by itself as a control. Plated on kanamycin/XGal plates.
- 7/24- Cotransformation failed. Likely due to too much plasmid, mixed with poor transformation.
