Team:Austin Texas/Week of July 23 to July 29

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== Design of Reporter Plasmid ==
== Design of Reporter Plasmid ==
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* Ben
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** Prepped pReporter and pReporter.permaflipped DNA.
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** '''PCR:'''
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*** pReporter: JE.rrnB.F, JE.GFPTerm.R
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*** pReporter.permaflipped: JE.rrnB.F, JE.GFPTerm.R
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*** '''Results:''' One correct-size band for pReporter.permaflipped; none for pReporter. Submitted for sequencing. Made frozen stock. Will retry colony PCR for pReporter.
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** '''Colony PCR:''' (retrying with Phusion + DMSO instead of Taq MM because I noticed JE.rrnB.F has poor secondary structure--probably why no products have shown up in these colony PCRs)
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*** pReporter: JE.rrnB.F, JE.GFPTerm.R
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*** '''Results:''' Correct-size band. Made frozen stock, prepped, and submitted for sequencing.
== Spinach Aptamer ==
== Spinach Aptamer ==

Revision as of 21:43, 31 July 2012

Contents

Notebook for week of July 23 to July 29

Design of Converter Plasmid

  • Razan
    • 7/24- Amplified pACYC backbone successfully with new primers for both control and converter plasmids.

Design of Reporter Plasmid

  • Ben
    • Prepped pReporter and pReporter.permaflipped DNA.
    • PCR:
      • pReporter: JE.rrnB.F, JE.GFPTerm.R
      • pReporter.permaflipped: JE.rrnB.F, JE.GFPTerm.R
      • Results: One correct-size band for pReporter.permaflipped; none for pReporter. Submitted for sequencing. Made frozen stock. Will retry colony PCR for pReporter.
    • Colony PCR: (retrying with Phusion + DMSO instead of Taq MM because I noticed JE.rrnB.F has poor secondary structure--probably why no products have shown up in these colony PCRs)
      • pReporter: JE.rrnB.F, JE.GFPTerm.R
      • Results: Correct-size band. Made frozen stock, prepped, and submitted for sequencing.

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

  • Peter
    • 7/23- Cotransformation of PRA301-ndmApro LacZ reporter plasmid (with spectinomycin reporter) and ORF1 -4,5,6 plasmid minipreps. Ran out of PRA301-ndmApro plasmid, so only ORF1-4,5 were cotransformed. ORF1- 6 was transformed by itself as a control. Plated on kanamycin/XGal plates.
    • 7/24- Cotransformation failed. Likely due to too much plasmid, mixed with poor transformation.

Inducible Odor

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