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Team:Austin Texas/Week of August 6 to August 12
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== Design of Converter Plasmid == | == Design of Converter Plasmid == | ||
| + | *Razan | ||
| + | **8/6- PCR on miniprepped DNA from gibson transformation colonies. | ||
| + | **8/9- Submitted control colony DNA and pconverter colony DNA for sequencing with confirmed sequence results for 2 control colonies and one converter colony. | ||
== Design of Reporter Plasmid == | == Design of Reporter Plasmid == | ||
| Line 28: | Line 31: | ||
== CBB5 Caffeine Inducible Promoters == | == CBB5 Caffeine Inducible Promoters == | ||
| + | *Peter | ||
| + | **8/6- Picked 2 colonies form ORF1-4 transformation plate. 1st was large, 2nd was small | ||
| + | **8/7- Glycerol stock saved of both ORF1-4 transformation colonies. Colony PCR began on both, with ORF1-4 plasmid as a control. | ||
| + | **8/8- Miller Assay of ndmA Promoter with ORF's from CBB5 using various Xanthine derivatives. | ||
| + | **8/9- More Miller Assays. Prepared new xanthine derivative stocks. Innoculated new Xanthine derivative cultures. | ||
| + | **8/10- Miller Assays of yesterday's cultures. | ||
== Inducible Odor == | == Inducible Odor == | ||
Latest revision as of 01:11, 3 October 2012
Contents |
Notebook for week of August 6 to August 12
Design of Converter Plasmid
- Razan
- 8/6- PCR on miniprepped DNA from gibson transformation colonies.
- 8/9- Submitted control colony DNA and pconverter colony DNA for sequencing with confirmed sequence results for 2 control colonies and one converter colony.
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 8/6- Picked 2 colonies form ORF1-4 transformation plate. 1st was large, 2nd was small
- 8/7- Glycerol stock saved of both ORF1-4 transformation colonies. Colony PCR began on both, with ORF1-4 plasmid as a control.
- 8/8- Miller Assay of ndmA Promoter with ORF's from CBB5 using various Xanthine derivatives.
- 8/9- More Miller Assays. Prepared new xanthine derivative stocks. Innoculated new Xanthine derivative cultures.
- 8/10- Miller Assays of yesterday's cultures.
