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Team:Austin Texas/Using the iGEM parts kit
From 2012.igem.org
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(Created page with "*[http://partsregistry.org/Help:Transformation_Protocol Transformation Protocol for Kit Parts] **After resuspending the DNA in the well transfer into 1.5 ml tube and enter into l...") |
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| + | *[http://partsregistry.org/Help:Distribution_Kits Instructions for Resuspending DNA from the Parts Kit] | ||
*[http://partsregistry.org/Help:Transformation_Protocol Transformation Protocol for Kit Parts] | *[http://partsregistry.org/Help:Transformation_Protocol Transformation Protocol for Kit Parts] | ||
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| + | === Special Notes === | ||
| + | * After resuspending the DNA in the well in 10 µl of H<sub>2</sub>O, transfer into 1.5 ml tube and enter into lab database. You'll only need 1-2 µl for your transformation, so we can save the remainder in case there are problems with the plasmid in the colony that you happen to pick. | ||
| + | * After you have colonies grown on the correct antibiotic plate, it is best practices to pick ''three'' colonies and save them as glycerol stocks. This way if the first one you miniprep is mutated, you can try these colonies first before retransforming. | ||
Revision as of 21:13, 7 June 2012
Special Notes
- After resuspending the DNA in the well in 10 µl of H2O, transfer into 1.5 ml tube and enter into lab database. You'll only need 1-2 µl for your transformation, so we can save the remainder in case there are problems with the plasmid in the colony that you happen to pick.
- After you have colonies grown on the correct antibiotic plate, it is best practices to pick three colonies and save them as glycerol stocks. This way if the first one you miniprep is mutated, you can try these colonies first before retransforming.
