Team:Austin Texas/Plasmids and Strains

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Revision as of 00:06, 13 June 2012 by Razan (Talk | contribs)

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Slot Name Description Source
iGEM 2012 Plasmid Box 1 2 cadA ASKA cadA plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 3 ldcC ASKA ldcC plasmid Barrick Lab

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Name Description Source
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab

ZombiE.coli parts

Plasmid Part Description Sequence Source
pReporter BBa_I714891 GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) File:BBa I714891.fasta.txt 2012 kit plate 2 (15L)
pReporter BBa_J06504 Monomeric RFP optimized for bacteria File:BBa J06504.fasta.txt 2012 kit plate 1 (13F)
pReporter BBa_K081009 LasI protein generator (TERM-) File:BBa S03967.fasta.txt 2012 kit plate 2 (10N)
pReporter BBa_J24679 RBS + LacI File:BBa J24679.fasta.txt 2012 kit plate 1 (7L)
pReporter BBa_B0015 Double terminator (BBa_B0010-BBa_B0012) File:BBa B0015.fasta.txt 2012 kit plate 1 (23L)
pConverter BBa_R0079 LasR Promoter 2012 kit plate 1 (12A)
pConverter BBa_C0079 LasR 2012 kit plate 1 (14J)
pConverter BBa_K082016 LacIM1 Parts Registry
pConverter BBa_R0010 LacI Promoter 2012 kit plate 1 (1D)
pConverter CRE pQL269 Plasmid