From 2012.igem.org
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
Box
| Slot
| Label
| Name
| Part
| Plasmid
| Source
| Added by
|
Plasmid Box 1
| 2
|
| cadA
|
| ASKA cadA plasmid
| Barrick Lab
|
|
Plasmid Box 1
| 3
|
| ldcC
|
| ASKA ldcC plasmid
| Barrick Lab
|
|
Plasmid Box 1
|
| IGM004
| GFP
| BBa_I714891
| pSB3K3
| 2012 kit plate 2 (15L)
| Ben
|
Plasmid Box 1
|
| IGM005
| RFP
| BBa_K081014
| pSB1AK3
| 2012 kit plate 3 (12O)
| Ben
|
Plasmid Box 1
|
| IGM006
| LasI
| BBa_K081009
| pSB1A2
| 2012 kit plate 2 (10N)
| Ben
|
Plasmid Box 1
|
| IGM007
| RBS + LacI
| BBa_J24679
| pSB1A2
| 2012 kit plate 1 (7L)
| Aurko
|
Plasmid Box 1
|
| IGM008
| Double terminator (BBa_B0010 - BBa_B0012)
| BBa_B0015
| pSB1AK3
| 2012 kit plate 1 (23L)
| Aurko
|
Plasmid Box 1
|
| IGM009
| J23100 promoter - sfGFP (Part:BBa_K515105)
| BBa_K515105
| pSB1C3
| 2012 kit plate 5 (1K)
|
|
Strain Database
All strains are in the back right rack of the –80°C freezer.
Box
| Slot
| Label
| Description
| Strain
| Source
| Added by
|
iGEM 2012 Box 1
| 2
| AG1
| Host strain of ASKA collection
|
| Barrick Lab
|
|
iGEM 2012 Box 1
| 3
| BW25113
| Host strain of Keio collection
|
| Barrick Lab
|
|
iGEM 2012 Box 1
| 4
| cadA
| AG1 + ASKA collection cadA plasmid, CamR
|
| Barrick Lab
|
|
iGEM 2012 Box 1
| 5
| ldcC
| AG1 + ASKA collection ldcC plasmid, CamR
|
| Barrick Lab
|
|
iGEM 2012 Box 1
| 6
| tnaA
| Keio collection knockout (version of odor-less E. coli), KanR
|
| Barrick Lab
|
|
iGEM 2012 Box 1
| 7
| IGM009
| crdASC (4864 bp)
| TOP10
| Barrick Lab
| Ben
|
iGEM 2012 Box 1
| 8
| IGM010
| crdASC (4943 bp)
| TOP10
| Barrick Lab
| Ben
|
iGEM 2012 Box 1
| 9
| IGM011
| crdASC (4864 bp)
| BL21(DE3)
| Barrick Lab
| Ben
|
iGEM 2012 Box 1
| 10
| IGM012
| crdASC (4943 bp)
| BL21(DE3)
| Barrick Lab
| Ben
|
iGEM 2012 Box 1
| 11
| IGM013
| BBa_K515105: J23100-sfGFP-pSB1C3(CamR)
| epi300
| Parts Kit
|
|
ZombiE.coli parts
Plasmid
| Part
| Description
| Source
|
pReporter
| BBa_I714891
| GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?)
| 2012 kit plate 2 (15L)
|
pReporter
| BBa_K081014
| RFP protein generator - PoPS->RFP
| 2012 kit plate 3 (12O)
|
pReporter
| BBa_K081009
| LasI protein generator (TERM-)
| 2012 kit plate 2 (10N)
|
pReporter
| BBa_J24679
| RBS + LacI
| 2012 kit plate 1 (7L)
|
pReporter
| BBa_B0015
| Double terminator (BBa_B0010-BBa_B0012)
| 2012 kit plate 1 (23L)
|
pConverter
| BBa_R0079
| LasR Promoter
| 2012 kit plate 1 (12A)
|
pConverter
| BBa_C0079
| LasR
| 2012 kit plate 1 (14J)
|
pConverter
| BBa_K082016
| LacIM1
| Parts Registry
|
pConverter
| BBa_R0010
| LacI Promoter
| 2012 kit plate 1 (1D)
|
pConverter
|
| CRE
| pQL269 Plasmid
|