Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

(Difference between revisions)
(Plasmid Database)
(Plasmid Database)
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{| cellpadding="5" border="1" cellspacing="0"
{| cellpadding="5" border="1" cellspacing="0"
! Box
! Box
-
! Slot
 
! Label
! Label
! Name
! Name
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| 2
 
|
|
| cadA
| cadA
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| 3
 
|  
|  
| ldcC
| ldcC
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM001
| IGM001
| LasR Promoter
| LasR Promoter
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM002
| IGM002
| LasR
| LasR
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM003
| IGM003
| Lac Promoter
| Lac Promoter
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM004
| IGM004
| GFP
| GFP
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM005
| IGM005
-
| <span style="text-decoration:line-through;">RFP</span> (use IGM021)
+
| <span style="text-decoration:line-through;">RFP</span> (use IGM021 instead)
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
| pSB1AK3
| pSB1AK3
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM006
| IGM006
| LasI
| LasI
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM007
| IGM007
| RBS + LacI
| RBS + LacI
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM008
| IGM008
| Double terminator (BBa_B0010 - BBa_B0012)
| Double terminator (BBa_B0010 - BBa_B0012)
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM009
| IGM009
| J23100 promoter - sfGFP (Part:BBa_K515105)
| J23100 promoter - sfGFP (Part:BBa_K515105)
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM014
| IGM014
| Plasmid pSB1A3 backbone with RFP Insert
| Plasmid pSB1A3 backbone with RFP Insert
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM015
| IGM015
| Plasmid pSB1C3 backbone with RFP Insert
| Plasmid pSB1C3 backbone with RFP Insert
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM016
| IGM016
| Plasmid pSB1K3 backbone with RFP Insert
| Plasmid pSB1K3 backbone with RFP Insert
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM017
| IGM017
| Plasmid pSB1T3 backbone with RFP Insert
| Plasmid pSB1T3 backbone with RFP Insert
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM021
| IGM021
| RFP
| RFP
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| pSB1A2
| pSB1A2
| 2012 kit plate 1 (13F)
| 2012 kit plate 1 (13F)
-
|
+
| Incorrect
| Ben
| Ben
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM022
| IGM022
|  
|  
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM023
| IGM023
|  
|  
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM024
| IGM024
|  
|  
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM025
| IGM025
|  
|  
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|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
|
 
| IGM026
| IGM026
| sfGFP
| sfGFP

Revision as of 02:03, 27 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Label Name Part Plasmid Source Sequence Added by
Plasmid Box 1 cadA ASKA cadA plasmid Barrick Lab
Plasmid Box 1 ldcC ASKA ldcC plasmid Barrick Lab
Plasmid Box 1 IGM001 LasR Promoter BBa_R0079 pSB1A2 2012 kit plate 1 (12A) Verified Razan
Plasmid Box 1 IGM002 LasR BBa_C0079 pSB3K3 2012 kit plate 1 (14J) Verified Razan
Plasmid Box 1 IGM003 Lac Promoter BBa_R0010 pSB1A2 2012 kit plate 1 (1D) Razan
Plasmid Box 1 IGM004 GFP BBa_I714891 pSB3K3 2012 kit plate 2 (15L) Verified Ben
Plasmid Box 1 IGM005 RFP (use IGM021 instead) BBa_K081014 pSB1AK3 2012 kit plate 3 (12O) Verified Ben
Plasmid Box 1 IGM006 LasI BBa_K081009 pSB1A2 2012 kit plate 2 (10N) Verified Ben
Plasmid Box 1 IGM007 RBS + LacI BBa_J24679 pSB1A2 2012 kit plate 1 (7L) Aurko
Plasmid Box 1 IGM008 Double terminator (BBa_B0010 - BBa_B0012) BBa_B0015 pSB1AK3 2012 kit plate 1 (23L) Aurko
Plasmid Box 1 IGM009 J23100 promoter - sfGFP (Part:BBa_K515105) BBa_K515105 pSB1C3 2012 kit plate 5 (1K)
Plasmid Box 1 IGM014 Plasmid pSB1A3 backbone with RFP Insert BBa_J04450 pSB1A3 2012 kit plate 1 (1G) Logan
Plasmid Box 1 IGM015 Plasmid pSB1C3 backbone with RFP Insert BBa_J04450 pSB1C3 2012 kit plate 1 (3A) Logan
Plasmid Box 1 IGM016 Plasmid pSB1K3 backbone with RFP Insert BBa_J04450 pSB1K3 2012 kit plate 1 (5A) Logan
Plasmid Box 1 IGM017 Plasmid pSB1T3 backbone with RFP Insert BBa_J04450 pSB1T3 2012 kit plate 1 (7A) Logan
Plasmid Box 1 IGM021 RFP BBa_J06504 pSB1A2 2012 kit plate 1 (13F) Incorrect Ben
Plasmid Box 1 IGM022 pAK.TAQ Jared Mike
Plasmid Box 1 IGM023 pET21.KOD Jared Mike
Plasmid Box 1 IGM024 pQL269 Jared Mike
Plasmid Box 1 IGM025 pACYC.T7.GFP Jared Mike
Plasmid Box 1 IGM026 sfGFP pET21 Razan

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Label Description Strain Source Added by
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab
iGEM 2012 Box 1 7 IGM009 crdASC (4864 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 8 IGM010 crdASC (4943 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 9 IGM011 crdASC (4864 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 10 IGM012 crdASC (4943 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 11 IGM013 BBa_K515105: J23100-sfGFP-pSB1C3(CamR) epi300 Parts Kit
iGEM 2012 Box 1 IGM014 pSB1A3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM015 pSB1C3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM016 pSB1K3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM017 pSB1T3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM018 pSB1A2 with LasR Promoter Bba_R0079 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM019 pSB2K3 with LasR Bba_C0079 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM020 pSB1A2 with Lac Promoter Bba_R0010 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM021 J23151_GFP_pSB3K3: Bba_I20270 TOP10 Parts Kit Erik
iGEM 2012 Box 1 IGM022 J23101_GFP_pSB3K3: Bba_I20260 TOP10 Parts Kit Erik

ZombiE.coli parts

Plasmid Part Description Source
pReporter BBa_I714891 GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) 2012 kit plate 2 (15L)
pReporter BBa_K081014 RFP protein generator - PoPS->RFP 2012 kit plate 3 (12O)
pReporter BBa_K081009 LasI protein generator (TERM-) 2012 kit plate 2 (10N)
pReporter BBa_J24679 RBS + LacI 2012 kit plate 1 (7L)
pReporter BBa_B0015 Double terminator (BBa_B0010-BBa_B0012) 2012 kit plate 1 (23L)
pConverter BBa_R0079 LasR Promoter 2012 kit plate 1 (12A)
pConverter BBa_C0079 LasR 2012 kit plate 1 (14J)
pConverter BBa_K082016 LacIM1 Parts Registry
pConverter BBa_R0010 LacI Promoter 2012 kit plate 1 (1D)
pConverter CRE pQL269 Plasmid