Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

(Difference between revisions)
(→ZombiE.coli parts)
(→ZombiE.coli parts)
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! Part
! Part
! Description
! Description
-
! Sequence
 
! Source
! Source
|-
|-
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| [http://partsregistry.org/Part:BBa_I714891 BBa_I714891]
| [http://partsregistry.org/Part:BBa_I714891 BBa_I714891]
| GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'')
| GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'')
-
| [[File:BBa_I714891.fasta.txt]]
 
| 2012 kit plate 2 (15L)
| 2012 kit plate 2 (15L)
|-
|-
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| [http://partsregistry.org/Part:BBa_J06504 BBa_J06504]
| [http://partsregistry.org/Part:BBa_J06504 BBa_J06504]
| Monomeric RFP optimized for bacteria
| Monomeric RFP optimized for bacteria
-
| [[File:BBa_J06504.fasta.txt]]
 
| 2012 kit plate 1 (13F)
| 2012 kit plate 1 (13F)
|-
|-
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| [http://partsregistry.org/Part:BBa_K081009 BBa_K081009]
| [http://partsregistry.org/Part:BBa_K081009 BBa_K081009]
| LasI protein generator (TERM-)
| LasI protein generator (TERM-)
-
| [[File:BBa S03967.fasta.txt]]
 
| 2012 kit plate 2 (10N)
| 2012 kit plate 2 (10N)
|-
|-
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| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
| RBS + LacI
| RBS + LacI
-
| [[File:BBa_J24679.fasta.txt]]
 
| 2012 kit plate 1 (7L)
| 2012 kit plate 1 (7L)
|-
|-
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| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
| Double terminator (BBa_B0010-BBa_B0012)
| Double terminator (BBa_B0010-BBa_B0012)
-
| [[File:BBa_B0015.fasta.txt]]
 
| 2012 kit plate 1 (23L)
| 2012 kit plate 1 (23L)
|-
|-
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| [http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
| [http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
| LasR Promoter
| LasR Promoter
-
|
 
| 2012 kit plate 1 (12A)
| 2012 kit plate 1 (12A)
|-
|-
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| [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
| [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
| LasR
| LasR
-
|
 
| 2012 kit plate 1 (14J)
| 2012 kit plate 1 (14J)
|-
|-
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| [http://partsregistry.org/Part:BBa_K082016 BBa_K082016]
| [http://partsregistry.org/Part:BBa_K082016 BBa_K082016]
| LacIM1
| LacIM1
-
|
 
| Parts Registry
| Parts Registry
|-
|-
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| [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
| [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
| LacI Promoter
| LacI Promoter
-
|
 
| 2012 kit plate 1 (1D)
| 2012 kit plate 1 (1D)
|-
|-
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|  
|  
| CRE
| CRE
-
|
 
| pQL269 Plasmid
| pQL269 Plasmid
|}
|}

Revision as of 20:11, 13 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Slot Name Description Source
iGEM 2012 Plasmid Box 1 2 cadA ASKA cadA plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 3 ldcC ASKA ldcC plasmid Barrick Lab

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Name Description Source
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab

ZombiE.coli parts

Plasmid Part Description Source
pReporter BBa_I714891 GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) 2012 kit plate 2 (15L)
pReporter BBa_J06504 Monomeric RFP optimized for bacteria 2012 kit plate 1 (13F)
pReporter BBa_K081009 LasI protein generator (TERM-) 2012 kit plate 2 (10N)
pReporter BBa_J24679 RBS + LacI 2012 kit plate 1 (7L)
pReporter BBa_B0015 Double terminator (BBa_B0010-BBa_B0012) 2012 kit plate 1 (23L)
pConverter BBa_R0079 LasR Promoter 2012 kit plate 1 (12A)
pConverter BBa_C0079 LasR 2012 kit plate 1 (14J)
pConverter BBa_K082016 LacIM1 Parts Registry
pConverter BBa_R0010 LacI Promoter 2012 kit plate 1 (1D)
pConverter CRE pQL269 Plasmid
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Plasmids_and_Strains"