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Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
(Difference between revisions)
Erik.quandt (Talk | contribs) (→Plasmid Database) |
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| Line 84: | Line 84: | ||
! Box | ! Box | ||
! Slot | ! Slot | ||
| - | ! | + | ! Label |
! Description | ! Description | ||
| + | ! Strain | ||
! Source | ! Source | ||
| + | ! Added by | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| Line 92: | Line 94: | ||
| AG1 | | AG1 | ||
| Host strain of ASKA collection | | Host strain of ASKA collection | ||
| + | | | ||
| Barrick Lab | | Barrick Lab | ||
| + | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| Line 98: | Line 102: | ||
| BW25113 | | BW25113 | ||
| Host strain of Keio collection | | Host strain of Keio collection | ||
| + | | | ||
| Barrick Lab | | Barrick Lab | ||
| + | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| Line 104: | Line 110: | ||
| cadA | | cadA | ||
| AG1 + ASKA collection cadA plasmid, CamR | | AG1 + ASKA collection cadA plasmid, CamR | ||
| + | | | ||
| Barrick Lab | | Barrick Lab | ||
| + | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| Line 110: | Line 118: | ||
| ldcC | | ldcC | ||
| AG1 + ASKA collection ldcC plasmid, CamR | | AG1 + ASKA collection ldcC plasmid, CamR | ||
| + | | | ||
| Barrick Lab | | Barrick Lab | ||
| + | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| Line 116: | Line 126: | ||
| tnaA | | tnaA | ||
| Keio collection knockout (version of odor-less E. coli), KanR | | Keio collection knockout (version of odor-less E. coli), KanR | ||
| + | | | ||
| Barrick Lab | | Barrick Lab | ||
| + | | | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| 7 | | 7 | ||
| IGM009 | | IGM009 | ||
| - | | crdASC (4864 bp) | + | | crdASC (4864 bp) |
| + | | TOP10 | ||
| Barrick Lab | | Barrick Lab | ||
| + | | Ben | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| 8 | | 8 | ||
| IGM010 | | IGM010 | ||
| - | | crdASC (4943 bp) | + | | crdASC (4943 bp) |
| + | | TOP10 | ||
| Barrick Lab | | Barrick Lab | ||
| + | | Ben | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| 9 | | 9 | ||
| IGM011 | | IGM011 | ||
| - | | crdASC (4864 bp) | + | | crdASC (4864 bp) |
| + | | BL21(DE3) | ||
| Barrick Lab | | Barrick Lab | ||
| + | | Ben | ||
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| 10 | | 10 | ||
| IGM012 | | IGM012 | ||
| - | | crdASC (4943 bp) | + | | crdASC (4943 bp) |
| + | | BL21(DE3) | ||
| Barrick Lab | | Barrick Lab | ||
| - | | | + | | Ben |
|- | |- | ||
| iGEM 2012 Box 1 | | iGEM 2012 Box 1 | ||
| 11 | | 11 | ||
| IGM013 | | IGM013 | ||
| - | | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) | + | | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) |
| + | | epi300 | ||
| Parts Kit | | Parts Kit | ||
| - | + | | | |
| + | |- | ||
|} | |} | ||
Revision as of 21:24, 19 June 2012
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Slot | Label | Name | Part | Plasmid | Source |
|---|---|---|---|---|---|---|
| iGEM 2012 Plasmid Box 1 | 2 | cadA | ASKA cadA plasmid | Barrick Lab | ||
| iGEM 2012 Plasmid Box 1 | 3 | ldcC | ASKA ldcC plasmid | Barrick Lab | ||
| iGEM 2012 Plasmid Box 1 | IGM004 | GFP | BBa_I714891 | pSB3K3 | 2012 kit plate 2 (15L) | |
| iGEM 2012 Plasmid Box 1 | IGM005 | RFP | BBa_K081014 | pSB1AK3 | 2012 kit plate 3 (12O) | |
| iGEM 2012 Plasmid Box 1 | IGM006 | LasI | BBa_K081009 | pSB1A2 | 2012 kit plate 2 (10N) | |
| iGEM 2012 Plasmid Box 1 | IGM007 | RBS + LacI | BBa_J24679 | pSB1A2 | 2012 kit plate 1 (7L) | |
| iGEM 2012 Plasmid Box 1 | IGM008 | Double terminator (BBa_B0010-BBa_B0012) | BBa_B0015 | pSB1AK3 | 2012 kit plate 1 (23L) | |
| iGEM 2012 Plasmid Box 1 | IGM009 | J23100 promoter - sfGFP (Part:BBa_K515105) | BBa_K515105 | pSB1C3 | 2012 kit plate 5 (1K) |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Label | Description | Strain | Source | Added by |
|---|---|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab | ||
| iGEM 2012 Box 1 | 7 | IGM009 | crdASC (4864 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 8 | IGM010 | crdASC (4943 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 9 | IGM011 | crdASC (4864 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 10 | IGM012 | crdASC (4943 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 11 | IGM013 | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) | epi300 | Parts Kit |
ZombiE.coli parts
| Plasmid | Part | Description | Source |
|---|---|---|---|
| pReporter | BBa_I714891 | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | 2012 kit plate 2 (15L) |
| pReporter | BBa_K081014 | RFP protein generator - PoPS->RFP | 2012 kit plate 3 (12O) |
| pReporter | BBa_K081009 | LasI protein generator (TERM-) | 2012 kit plate 2 (10N) |
| pReporter | BBa_J24679 | RBS + LacI | 2012 kit plate 1 (7L) |
| pReporter | BBa_B0015 | Double terminator (BBa_B0010-BBa_B0012) | 2012 kit plate 1 (23L) |
| pConverter | BBa_R0079 | LasR Promoter | 2012 kit plate 1 (12A) |
| pConverter | BBa_C0079 | LasR | 2012 kit plate 1 (14J) |
| pConverter | BBa_K082016 | LacIM1 | Parts Registry |
| pConverter | BBa_R0010 | LacI Promoter | 2012 kit plate 1 (1D) |
| pConverter | CRE | pQL269 Plasmid |
