Revision as of 20:55, 13 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box	Slot	Name	Description	Source
iGEM 2012 Plasmid Box 1	2	cadA	ASKA cadA plasmid	Barrick Lab
iGEM 2012 Plasmid Box 1	3	ldcC	ASKA ldcC plasmid	Barrick Lab

All strains are in the back right rack of the –80°C freezer.

Box	Slot	Name	Description	Source
iGEM 2012 Box 1	2	AG1	Host strain of ASKA collection	Barrick Lab
iGEM 2012 Box 1	3	BW25113	Host strain of Keio collection	Barrick Lab
iGEM 2012 Box 1	4	cadA	AG1 + ASKA collection cadA plasmid, CamR	Barrick Lab
iGEM 2012 Box 1	5	ldcC	AG1 + ASKA collection ldcC plasmid, CamR	Barrick Lab
iGEM 2012 Box 1	6	tnaA	Keio collection knockout (version of odor-less E. coli), KanR	Barrick Lab

Plasmid	Part	Description	Source
pReporter	BBa_I714891	GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?)	2012 kit plate 2 (15L)
pReporter	BBa_K081014	RFP protein generator - PoPS->RFP	2012 kit plate 3 (12O)
pReporter	BBa_K081009	LasI protein generator (TERM-)	2012 kit plate 2 (10N)
pReporter	BBa_J24679	RBS + LacI	2012 kit plate 1 (7L)
pReporter	BBa_B0015	Double terminator (BBa_B0010-BBa_B0012)	2012 kit plate 1 (23L)
pConverter	BBa_R0079	LasR Promoter	2012 kit plate 1 (12A)
pConverter	BBa_C0079	LasR	2012 kit plate 1 (14J)
pConverter	BBa_K082016	LacIM1	Parts Registry
pConverter	BBa_R0010	LacI Promoter	2012 kit plate 1 (1D)
pConverter		CRE	pQL269 Plasmid

@@ Line 82: / Line 82: @@
 |-
 | pReporter
-| [http://partsregistry.org/Part:BBa_J06504 BBa_J06504]
+| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
-| Monomeric RFP optimized for bacteria
+| RFP protein generator - PoPS->RFP
-| 2012 kit plate 1 (13F)
+| 2012 kit plate 3 (12O)
 |-
 | pReporter