"
Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
(Difference between revisions)
(→ZombiE.coli parts) |
|||
| Line 80: | Line 80: | ||
| BBa_I714891 | | BBa_I714891 | ||
| GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'') | | GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'') | ||
| - | | | + | | [[File:BBa_I714891.fasta.txt]] |
| 2012 kit plate 2 (15L) | | 2012 kit plate 2 (15L) | ||
|- | |- | ||
| Line 86: | Line 86: | ||
| BBa_J06504 | | BBa_J06504 | ||
| Monomeric RFP optimized for bacteria | | Monomeric RFP optimized for bacteria | ||
| - | | | + | | [[File:BBa_J06504.fasta.txt]] |
| 2012 kit plate 1 (13F) | | 2012 kit plate 1 (13F) | ||
|- | |- | ||
| Line 92: | Line 92: | ||
| BBa_K081009 | | BBa_K081009 | ||
| LasI protein generator (TERM-) | | LasI protein generator (TERM-) | ||
| - | | | + | | [[File:BBa S03967.fasta.txt]] |
| 2012 kit plate 2 (10N) | | 2012 kit plate 2 (10N) | ||
|} | |} | ||
Revision as of 21:00, 9 June 2012
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Slot | Name | Description | Source |
|---|---|---|---|---|
| iGEM 2012 Plasmid Box 1 | 2 | cadA | ASKA cadA plasmid | Barrick Lab |
| iGEM 2012 Plasmid Box 1 | 3 | ldcC | ASKA ldcC plasmid | Barrick Lab |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Name | Description | Source |
|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab |
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab |
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab |
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab |
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab |
ZombiE.coli parts
| Plasmid | Part | Description | Sequence | Source |
|---|---|---|---|---|
| pReporter | BBa_I714891 | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | File:BBa I714891.fasta.txt | 2012 kit plate 2 (15L) |
| pReporter | BBa_J06504 | Monomeric RFP optimized for bacteria | File:BBa J06504.fasta.txt | 2012 kit plate 1 (13F) |
| pReporter | BBa_K081009 | LasI protein generator (TERM-) | File:BBa S03967.fasta.txt | 2012 kit plate 2 (10N) |
