Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

(Difference between revisions)
(→Strain Database)
Line 66: Line 66:
|-
|-
 +
|}
 +
 +
= ZombiE.coli parts =
 +
 +
{| cellpadding="5" border="1" cellspacing="0"
 +
! Plasmid
 +
! Part
 +
! Description
 +
! Sequence
 +
! Source
 +
|-
 +
| pReporter
 +
| BBa_I714891
 +
| GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'')
 +
|
 +
| 2012 kit plate 2 (15L)
 +
|-
 +
| pReporter
 +
| BBa_J06504
 +
| Monomeric RFP optimized for bacteria
 +
|
 +
| 2012 kit plate 1 (13F)
 +
|-
 +
| pReporter
 +
| BBa_K081009
 +
| LasI protein generator (TERM-)
 +
|
 +
| 2012 kit plate 2 (10N)
|}
|}

Revision as of 20:46, 9 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Slot Name Description Source
iGEM 2012 Plasmid Box 1 2 cadA ASKA cadA plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 3 ldcC ASKA ldcC plasmid Barrick Lab

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Name Description Source
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab

ZombiE.coli parts

Plasmid Part Description Sequence Source
pReporter BBa_I714891 GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) 2012 kit plate 2 (15L)
pReporter BBa_J06504 Monomeric RFP optimized for bacteria 2012 kit plate 1 (13F)
pReporter BBa_K081009 LasI protein generator (TERM-) 2012 kit plate 2 (10N)
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Plasmids_and_Strains"