Team:Austin Texas

From 2012.igem.org

(Difference between revisions)
 
(91 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** (Keeping this here temporarily --bs)
+
{{Template:Austin_Texas/Stylesheet}}
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
 
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
<ul class="cssmenu" style="float:left;">
-
This is a template page. READ THESE INSTRUCTIONS.
+
<li class="home"><a href="/Team:Austin_Texas" class="selected" title="home"><span class="displace">Home</span></a></li>
-
</div>
+
<li class="team"><a href="/Team:Austin_Texas/Team" title="team"><span class="displace">Team</span></a></li>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
<li class="official_team_profile"><a href="https://igem.org/Team.cgi?year=2012&team_name=Austin_Texas" title="official_team_profile"><span class="displace">Official Team Profile</span></a></li>
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
<li class="human_practices"><a href="/Team:Austin_Texas/ZombiE_coli#Human_Practices" title="Human Practices"><span class="displace">Human Practices</span></a></li>
-
</div>
+
<li class="Caffeinated_coli"><a href="/Team:Austin_Texas/Caffeinated_coli" title="Caffeinated_coli"><span class="displace">Caffeinated coli</span></a></li>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
<li class="ZombiE_coli"><a href="/Team:Austin_Texas/ZombiE_coli" title="ZombiE_coli"><span class="displace">ZombiE.coli</span></a></li>
-
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
+
<li class="Spinach_reporter"><a href="/Team:Austin_Texas/Spinach_reporter" title="Spinach_reporter"><span class="displace">Spinach reporter</span></a></li>
-
</div>
+
<li class="notebook"><a href="/Team:Austin_Texas/Notebook" title="notebook"><span class="displace">Notebook</span></a></li>
-
</div>
+
<li class="parts_submitted"><a href="/Team:Austin_Texas/Parts" title="parts_submitted"><span class="displace">Parts Submitted</span></a></li>
 +
<li class="safety"><a href="/Team:Austin_Texas/Safety" title="safety"><span class="displace">Safety</span></a></li>
 +
<li class="attributions"><a href="/Team:Austin_Texas/Team#Attributions" title="attributions"><span class="displace">Attributions</span></a></li>
 +
</ul>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2012/1/16/University_of_texas_logo.jpg" alt="University of Texas at Austin logo" class="ut_logo" />
 +
 
</html>
</html>
 +
[[File:CokeGrowth.png|335px|left]]
 +
[[File:DietCokeGrowth.png|350px|right]]
 +
[[File:UTAustinTower.jpg|x355px|center]]
 +
 +
 +
= Project Caffeinated coli =
 +
 +
<html><a href="/Team:Austin_Texas/Caffeinated_coli"><img src="https://static.igem.org/mediawiki/2012/d/d1/Caffeinated_Coli.jpeg"; alt="Caffeinated Coli"; width="170px"; height="250px"; style="float:left; padding:3px; clear:right;"/></a></html>
 +
 +
 +
 +
The widespread use of caffeine (1,3,7–trimethylxanthine) and other methylxanthines in beverages and pharmaceuticals has led to significant environmental pollution. We have developed a novel detection and bioremediation strategy for caffeine contamination by refactoring the methylxanthine degradation operon native to ''Pseudomonas putida'' CBB5. ''Escherichia coli'' cells with this synthetic operon degrade caffeine by N-demethylation to the guanine precursor, xanthine. Cells deficient in guanine biosynthesis and containing our refactored operon were addicted to caffeine; their growth density was limited by the availability of caffeine. Remarkably, they were able to sense the caffeine content of several common beverages. Characterization of nearby genes in the ''P. putida'' operon revealed a potential methylxanthine regulatory system for use in biological circuit design. The synthetic N-demethylation operon could be useful for cheaply producing pharmaceuticals or precursor molecules and for detoxifying waste so that it can be recycled into animal feed and biofuels.
 +
 +
<br /><br /><br /><br /><br />
 +
 +
= Project ZombiE.coli =
 +
 +
 +
<html><a href="/Team:Austin_Texas/ZombiE_coli"><img src="https://static.igem.org/mediawiki/2012/a/a9/Austin_Texas_logo.png"; alt="ZombiE.coli"; width="170px"; height="250px"; style="float:left; padding:3px; clear:right;"/></a></html>
 +
 +
 +
 +
UT’s ZombiE.coli project aims to a develop a tightly regulated genetic switch that is triggered by bacterial quorum signaling and leads to feed-forward propagation of the genetic output in the form of red or green fluorescence as well as amplification of quorum signaling. The switch relies on simple one-way Cre/loxP recombination combined with native quorum signaling to provide us with a system that models transmissible disease spread between populations. We have likened this to an airborne zombie epidemic, in which an “infected” zombie cell is capable of restructuring the genes of a normal cell, turning it into a flesh-hungry counterpart. This system will be useful not only as a simple disease outbreak model for intermediate-level biology education, but also, could provide new insights to how bacterial populations communicate in three dimensions and under different genetic backgrounds.
 +
 +
<br /><br /><br /><br /><br />
 +
 +
= Project PopeyE.coli =
 +
 +
<html><a href="/Team:Austin_Texas/Spinach_reporter"><img src="https://static.igem.org/mediawiki/2012/8/85/Spinach_icon.png"; alt="PopeyEcoli"; width="170px"; style="float:left; padding:3px; clear:right;"/></a></html>
 +
 +
 +
 +
In an effort to improve the efficiency, ease, and quality of promoter and RBS strength measurements, we focused on developing a dual fluorescence reporter for simultaneous monitoring both transcription and translation. To measure both processes separately, two fluorescent reporters, the Spinach aptamer and mCherry red fluorescent protein, were assembled into a single construct. The Spinach-mCherry dual reporter is a unique concept; Spinach is a short RNA aptamer that binds to its ligand, DFHBI, and allows it to emit green fluorescence similar to GFP. This gives insight into the direct production of the mCherry-encoding mRNA without the need to wait for protein folding and maturation of the fluorophore. This technique attempted to expand upon current efforts to measure promoter strength relative to a reference standard used by the iGEM community.
-
*** End of the alert box *** -->
 
<html>
<html>
 +
<br /><br /><br /><br />
 +
<center>
 +
<a href="http://www.geneious.com/"><img src="https://static.igem.org/mediawiki/2012/d/db/Geneious.png" alt="Geneious logo" width="150px" height="62px" /></a>
 +
<a href="http://www.neb.com"><img src="https://static.igem.org/mediawiki/2012/d/d6/Austin_Texas_NEB_logo.jpeg" alt="NEB logo" width="150px" height="58px" /></a>
 +
<a href="http://www.epochlifescience.com"><img src="https://static.igem.org/mediawiki/2012/c/c6/Austin_Texas_Epoch_logo.jpg" alt="Epoch logo" width="150px" height="65px" /></a>
 +
<a href="http://cssb.utexas.edu"><img src="https://static.igem.org/mediawiki/2012/4/43/UT_Austin_CSSB.jpg" alt="UT Austin CSSB logo" width="150px" height="65px" /></a>
 +
<a href="http://cns.utexas.edu"><img src="https://static.igem.org/mediawiki/2012/e/ec/UT_Austin_CNS.png" alt="UT Austin CNS logo" width="150px" height="65px" /></a>
-
<iframe src="https://www.google.com/calendar/embed?height=600&amp;wkst=1&amp;bgcolor=%23FFFFFF&amp;src=utexas.edu_j1fp852vjcsl1gof75qfoq9n4c%40group.calendar.google.com&amp;color=%232F6309&amp;ctz=America%2FChicago" style=" border-width:0 " width="600" height="400" frameborder="0" scrolling="no"></iframe>
+
</center>
 +
<div id="footer" />
</html>
</html>
 +
<!-- this is commented out
{|align="justify"
{|align="justify"
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
-
|[[Image:Austin_Texas_logo.png|200px|right|frame]]
+
|
|-
|-
|
|
Line 32: Line 79:
|-
|-
|
|
-
|align="center"|[[Team:Austin_Texas | Team Austin_Texas]]
+
|
|}
|}
-
<!--- The Mission, Experiments --->
+
-->
-
 
+
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
-
!align="center"|[[Team:Austin_Texas|Home]]
+
-
!align="center"|[[Team:Austin_Texas/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Austin_Texas Official Team Profile]
+
-
!align="center"|[[Team:Austin_Texas/Project|Project]]
+
-
!align="center"|[[Team:Austin_Texas/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Austin_Texas/Modeling|Modeling]]
+
-
!align="center"|[[Team:Austin_Texas/Notebook|Notebook]]
+
-
!align="center"|[[Team:Austin_Texas/Safety|Safety]]
+
-
!align="center"|[[Team:Austin_Texas/Attributions|Attributions]]
+
-
|}
+

Latest revision as of 16:21, 29 May 2013

CokeGrowth.png
DietCokeGrowth.png
UTAustinTower.jpg


Project Caffeinated coli

Caffeinated Coli


The widespread use of caffeine (1,3,7–trimethylxanthine) and other methylxanthines in beverages and pharmaceuticals has led to significant environmental pollution. We have developed a novel detection and bioremediation strategy for caffeine contamination by refactoring the methylxanthine degradation operon native to Pseudomonas putida CBB5. Escherichia coli cells with this synthetic operon degrade caffeine by N-demethylation to the guanine precursor, xanthine. Cells deficient in guanine biosynthesis and containing our refactored operon were addicted to caffeine; their growth density was limited by the availability of caffeine. Remarkably, they were able to sense the caffeine content of several common beverages. Characterization of nearby genes in the P. putida operon revealed a potential methylxanthine regulatory system for use in biological circuit design. The synthetic N-demethylation operon could be useful for cheaply producing pharmaceuticals or precursor molecules and for detoxifying waste so that it can be recycled into animal feed and biofuels.






Project ZombiE.coli

ZombiE.coli


UT’s ZombiE.coli project aims to a develop a tightly regulated genetic switch that is triggered by bacterial quorum signaling and leads to feed-forward propagation of the genetic output in the form of red or green fluorescence as well as amplification of quorum signaling. The switch relies on simple one-way Cre/loxP recombination combined with native quorum signaling to provide us with a system that models transmissible disease spread between populations. We have likened this to an airborne zombie epidemic, in which an “infected” zombie cell is capable of restructuring the genes of a normal cell, turning it into a flesh-hungry counterpart. This system will be useful not only as a simple disease outbreak model for intermediate-level biology education, but also, could provide new insights to how bacterial populations communicate in three dimensions and under different genetic backgrounds.






Project PopeyE.coli

PopeyEcoli


In an effort to improve the efficiency, ease, and quality of promoter and RBS strength measurements, we focused on developing a dual fluorescence reporter for simultaneous monitoring both transcription and translation. To measure both processes separately, two fluorescent reporters, the Spinach aptamer and mCherry red fluorescent protein, were assembled into a single construct. The Spinach-mCherry dual reporter is a unique concept; Spinach is a short RNA aptamer that binds to its ligand, DFHBI, and allows it to emit green fluorescence similar to GFP. This gives insight into the direct production of the mCherry-encoding mRNA without the need to wait for protein folding and maturation of the fluorophore. This technique attempted to expand upon current efforts to measure promoter strength relative to a reference standard used by the iGEM community.





Geneious logo NEB logo Epoch logo UT Austin CSSB logo UT Austin CNS logo