From 2012.igem.org
July 2012
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August 2012
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September 2012
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October 2012
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http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
June 07
June 08
Transformation results
puc19: growth
negative control: no growth
lacZ, lacO: possible small colonies
liquid culture in amp media (100 ug / ml):
no growth of lacZ, lacO
growth of puc19
June 12
DH5a Competent Cell Prep
Streak plated cells on LB no amp plate, let grow overnight
June 13
Transformation (LSE)
Transformation (istb4, Abhi)
Transformed DNA:
Cells:
Protocol from:
Controls:
DH5a Chemically Competent cell prep
Grew 2 seed colonies from streak plate in LB no amp
Grew controls to test for contamination
Both Seed colonies grew, no contamination present
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