Team:Arizona State/Notebook

From 2012.igem.org

(Difference between revisions)
Line 71: Line 71:
* Streak plated prepared competent cells on LB no amp plate
* Streak plated prepared competent cells on LB no amp plate
** Colonies observed
** Colonies observed
 +
 +
==June 19==
 +
* Transformation (LSE)
 +
** Transformed DNA:
 +
*** T7 promoter BBa_I712074
 +
*** Constitutive promoter BBa_J23102
 +
** Cells: DH5 alpha (donated)
 +
** Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
 +
** Controls: puc19, no DNA
 +
** Plated 2 copies of each (100 ul, 250 ul) on LB amp plates.
 +
*Made 50 LB Amp plates.
 +
 +
==June 20==
 +
* Plated negative control on LB Amp plate
 +
* Liquid cultures of T7 promoter and constitutive promoter
 +
* Transformation (LSE)
 +
** Transformed DNA:
 +
*** RBS (well 1:1H BBa_B0030)
 +
*** TetR GFP (well 2:8A Part:BBa_I13522)
 +
** Cells: DH5 alpha (donated)
 +
** Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
 +
** Controls: puc19, no DNA

Revision as of 01:32, 1 October 2012


June 2012
S M T W T F S
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
             
July 2012
S M T W T F S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31        
             
August 2012
S M T W T F S
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31  
             
September 2012
S M T W T F S
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30            
October 2012
S M T W T F S
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31      
             


Note from Dr. Haynes: Rohit, please transfer all of the Notebook entries from the OWW Wiki
http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
To this page. Follow the format below. I added a couple of sections to help you get started.

June 07

June 08

  • Transformation results
    • puc19: growth
    • negative control: no growth
    • lacZ, lacO: possible small colonies
  • liquid culture in amp media (100 ug / ml):
    • no growth of lacZ, lacO
    • growth of puc19

June 12

  • DH5a Competent Cell Prep
    • Streak plated cells on LB no amp plate, let grow overnight

June 13

  • Transformation (LSE)
  • Transformation (istb4, Abhi)
    • Transformed DNA:
    • Cells:
    • Protocol from:
    • Controls:
  • DH5a Chemically Competent cell prep
    • Grew 2 seed colonies from streak plate in LB no amp
    • Grew controls to test for contamination
      • Both Seed colonies grew, no contamination present

June 14

  • Competent cell prep
    • Prepared CaCl2 buffer solution and CaCl2 glycerol buffer solution
    • Grew seed colony in 400mL LB no amp

June 15

  • Competent cell prep
    • Centrifuged falcon test tubes containing liquid colonies
    • Resuspended in CaCl2 buffer solution and incubated for 15 mins
    • Centrifuged and resuspended in CaCl2 glycerol buffer solution
    • Chilled overnight

June 16

  • Competent cell prep
    • Aliquotted 200uL into test tubes
    • Stored in -80C

June 17

  • Streak plated prepared competent cells on LB no amp plate
    • Colonies observed

June 19

June 20

Retrieved from "http://2012.igem.org/Team:Arizona_State/Notebook"