Team:Arizona State/Notebook

From 2012.igem.org

(Difference between revisions)
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** Grew controls to test for contamination
** Grew controls to test for contamination
*** Both Seed colonies grew, no contamination present
*** Both Seed colonies grew, no contamination present
 +
 +
==June 14==
 +
* Competent cell prep
 +
** Prepared CaCl2 buffer solution and CaCl2 glycerol buffer solution
 +
** Grew seed colony in 400mL LB no amp
 +
 +
==June 15==
 +
* Competent cell prep
 +
** Centrifuged falcon test tubes containing liquid colonies
 +
** Resuspended in CaCl2 buffer solution and incubated for 15 mins
 +
** Centrifuged and resuspended in CaCl2 glycerol buffer solution
 +
** Chilled overnight
 +
 +
==June 16==
 +
* Competent cell prep
 +
** Aliquotted 200uL into test tubes
 +
** Stored in -80C
 +
 +
==June 17==
 +
* Streak plated prepared competent cells on LB no amp plate
 +
** Colonies observed

Revision as of 01:29, 1 October 2012


June 2012
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July 2012
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August 2012
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September 2012
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October 2012
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Note from Dr. Haynes: Rohit, please transfer all of the Notebook entries from the OWW Wiki
http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
To this page. Follow the format below. I added a couple of sections to help you get started.

June 07

June 08

  • Transformation results
    • puc19: growth
    • negative control: no growth
    • lacZ, lacO: possible small colonies
  • liquid culture in amp media (100 ug / ml):
    • no growth of lacZ, lacO
    • growth of puc19

June 12

  • DH5a Competent Cell Prep
    • Streak plated cells on LB no amp plate, let grow overnight

June 13

  • Transformation (LSE)
  • Transformation (istb4, Abhi)
    • Transformed DNA:
    • Cells:
    • Protocol from:
    • Controls:
  • DH5a Chemically Competent cell prep
    • Grew 2 seed colonies from streak plate in LB no amp
    • Grew controls to test for contamination
      • Both Seed colonies grew, no contamination present

June 14

  • Competent cell prep
    • Prepared CaCl2 buffer solution and CaCl2 glycerol buffer solution
    • Grew seed colony in 400mL LB no amp

June 15

  • Competent cell prep
    • Centrifuged falcon test tubes containing liquid colonies
    • Resuspended in CaCl2 buffer solution and incubated for 15 mins
    • Centrifuged and resuspended in CaCl2 glycerol buffer solution
    • Chilled overnight

June 16

  • Competent cell prep
    • Aliquotted 200uL into test tubes
    • Stored in -80C

June 17

  • Streak plated prepared competent cells on LB no amp plate
    • Colonies observed