Team:Arizona State/Notebook

From 2012.igem.org

(Difference between revisions)

Revision as of 01:26, 1 October 2012

June 2012
S	M	T	W	T	F	S
					1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30

July 2012
S	M	T	W	T	F	S
1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30	31

August 2012
S	M	T	W	T	F	S
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31

September 2012
S	M	T	W	T	F	S
						1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30

October 2012
S	M	T	W	T	F	S
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30	31

Note from Dr. Haynes: Rohit, please transfer all of the Notebook entries from the OWW Wiki
http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
To this page. Follow the format below. I added a couple of sections to help you get started.

June 07

Transformation (LSE)
- Transformed DNA:
  - lacZ (well 4:12G, I732019)
  - p + lacO (well 1:6G, R0011)
- Cells: neb10beta (donated)
- Protocol from: http://www.neb.com/nebecomm/products/productc3019.asp
- Controls: puc19, no DNA (8 plates)

June 08

Transformation results
- puc19: growth
- negative control: no growth
- lacZ, lacO: possible small colonies
liquid culture in amp media (100 ug / ml):
- no growth of lacZ, lacO
- growth of puc19

June 12

DH5a Competent Cell Prep
- Streak plated cells on LB no amp plate, let grow overnight

June 13

Transformation (LSE)
- Transformed DNA:
  - lacZ (well 4:12G, I732019)
  - p + lacO (well 1:6G, R0011)
- Cells: DH5 alpha (donated)
- Protocol from: http://openwetware.org/wiki/Haynes:Assembly101 (30 minute transformation)
- Controls: puc19, no DNA (8 plates)
Transformation (istb4, Abhi)
- Transformed DNA:
- Cells:
- Protocol from:
- Controls:
DH5a Chemically Competent cell prep
- Grew 2 seed colonies from streak plate in LB no amp
- Grew controls to test for contamination
  - Both Seed colonies grew, no contamination present

@@ Line 32: / Line 32: @@
 * DH5a Competent Cell Prep
 ** Streak plated cells on LB no amp plate, let grow overnight
+==June 13==
+* Transformation (LSE)
+** Transformed DNA:
+*** lacZ (well 4:12G, I732019)
+*** p + lacO (well 1:6G, R0011)
+** Cells: DH5 alpha (donated)
+** Protocol from: http://openwetware.org/wiki/Haynes:Assembly101 (30 minute transformation)
+** Controls: puc19, no DNA (8 plates)
+* Transformation (istb4, Abhi)
+** Transformed DNA:
+** Cells:
+** Protocol from:
+** Controls:
+* DH5a Chemically Competent cell prep
+** Grew 2 seed colonies from streak plate in LB no amp
+** Grew controls to test for contamination
+*** Both Seed colonies grew, no contamination present