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Team:Amsterdam/shadow/project/overview
From 2012.igem.org
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| - | <p><h4><b>Summary</b></h4> | + | <p> |
| + | <h4><b>Summary</b></h4> | ||
A chosen sensor gets activated which then promotes transcription of its specific coupled phusion protein, consisting of a ZN-finger and a methyltransferase (ZN-MTase). <br><br> | A chosen sensor gets activated which then promotes transcription of its specific coupled phusion protein, consisting of a ZN-finger and a methyltransferase (ZN-MTase). <br><br> | ||
| - | Newly translated ZN-MTase moves to and binds to its specific ZN-finger motif binding site and correlating MTase binding site on the introduced plasmid, allowing the MT to methylate its specific motif which is closely located to the Zn-finger binding site. One point of methylation corresponds with 1 bit of information stored on the plasmid.</p> | + | Newly translated ZN-MTase moves to and binds to its specific ZN-finger motif binding site and correlating MTase binding site on the introduced plasmid, allowing the MT to methylate its specific motif which is closely located to the Zn-finger binding site. One point of methylation corresponds with 1 bit of information stored on the plasmid.<br><br> |
| + | |||
| + | <h4><b>Storage<b></h4> | ||
| + | <b>Digest</b><br> | ||
| + | A methylation dependent restriction enzyme (RE) with the same motif as the MTase in the ZN-MTase is subsequently introduced in the cell. Depending on whether its restriction site is methylated or not it will be able to digest the DNA.<br><br> | ||
| + | |||
| + | Read out of the data will be done using PCR and gel electrophoresis and analysis of the band lengths observed showing whether digestion has occurred. This way a Boolean (true or false) for the presence of each signal in E. coli’s environment is stored. | ||
| + | </p> | ||
</div> | </div> | ||
</body> | </body> | ||
Revision as of 09:54, 6 June 2012
Summary
A chosen sensor gets activated which then promotes transcription of its specific coupled phusion protein, consisting of a ZN-finger and a methyltransferase (ZN-MTase).Newly translated ZN-MTase moves to and binds to its specific ZN-finger motif binding site and correlating MTase binding site on the introduced plasmid, allowing the MT to methylate its specific motif which is closely located to the Zn-finger binding site. One point of methylation corresponds with 1 bit of information stored on the plasmid.
Storage
DigestA methylation dependent restriction enzyme (RE) with the same motif as the MTase in the ZN-MTase is subsequently introduced in the cell. Depending on whether its restriction site is methylated or not it will be able to digest the DNA.
Read out of the data will be done using PCR and gel electrophoresis and analysis of the band lengths observed showing whether digestion has occurred. This way a Boolean (true or false) for the presence of each signal in E. coli’s environment is stored.
