Team:Amsterdam/safety/components
From 2012.igem.org
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Revision as of 21:38, 31 July 2012
Overview of used components
Plasmids
Supplied by the iGEM distribution kit 2012pSB1C3
J63009
pSB4C3
pSB3C5
pSB1AT3
(GFP plasmid)
Vectors
Supplied by the iGEM distribution kit 2012J45320
(GFP?)
Ordered M.ScaI synthesized vector
Chemicals
IPTGEtBr
Chloramphenicol
Ampicilin
Kanamycin
Substances
AGARLB
Bacteria
DH5-alphaKits
Qiagen mini-prepQiagen Gel extraction
Qiagen PCR purification
Methods
Gibson AssemblyUva/VU and RIVM safety (GMO’s)