Team:Amsterdam/safety/components
From 2012.igem.org
(Difference between revisions)
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<h2>Plasmids</h2> | <h2>Plasmids</h2> | ||
- | Supplied by the iGEM distribution kit 2012 | + | Supplied by the iGEM distribution kit 2012<br> |
- | pSB1C3 | + | pSB1C3<br> |
- | J63009 | + | J63009<br> |
- | pSB4C3 | + | pSB4C3<br> |
- | pSB3C5 | + | pSB3C5<br> |
- | pSB1AT3 | + | pSB1AT3<br> |
- | (GFP plasmid) | + | (GFP plasmid)<br><br> |
<h2>Vectors</h2> | <h2>Vectors</h2> | ||
- | Supplied by the iGEM distribution kit 2012 | + | Supplied by the iGEM distribution kit 2012<br> |
- | J45320 | + | J45320<br> |
- | (GFP?) | + | (GFP?)<br> |
- | Ordered M.ScaI synthesized vector | + | Ordered M.ScaI synthesized vector<br><br> |
<h2>Chemicals</h2> | <h2>Chemicals</h2> | ||
- | IPTG | + | IPTG<br> |
- | EtBr | + | EtBr<br> |
- | Chloramphenicol | + | Chloramphenicol<br> |
- | Ampicilin | + | Ampicilin<br> |
- | Kanamycin | + | Kanamycin<br><br> |
<h2>Substances</h2> | <h2>Substances</h2> | ||
- | AGAR | + | AGAR<br> |
- | LB | + | LB<br><br> |
<h2>Bacteria</h2> | <h2>Bacteria</h2> | ||
- | DH5-alpha | + | DH5-alpha<br><br> |
<h2>Kits</h2> | <h2>Kits</h2> | ||
- | Qiagen mini-prep | + | Qiagen mini-prep<br> |
- | Qiagen Gel extraction | + | Qiagen Gel extraction<br> |
- | Qiagen PCR purification | + | Qiagen PCR purification<br><br> |
<h2>Methods</h2> | <h2>Methods</h2> | ||
- | Gibson Assembly | + | Gibson Assembly<br><br> |
Uva/VU and RIVM safety (GMO’s) | Uva/VU and RIVM safety (GMO’s) |
Revision as of 21:25, 31 July 2012
Overview of used plasmids, vectors, chemicals and substances
Plasmids
Supplied by the iGEM distribution kit 2012pSB1C3
J63009
pSB4C3
pSB3C5
pSB1AT3
(GFP plasmid)
Vectors
Supplied by the iGEM distribution kit 2012J45320
(GFP?)
Ordered M.ScaI synthesized vector
Chemicals
IPTGEtBr
Chloramphenicol
Ampicilin
Kanamycin
Substances
AGARLB
Bacteria
DH5-alphaKits
Qiagen mini-prepQiagen Gel extraction
Qiagen PCR purification
Methods
Gibson AssemblyUva/VU and RIVM safety (GMO’s)