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Team:Amsterdam/project/molecular design
From 2012.igem.org
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function readerText(){ | function readerText(){ | ||
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| - | $('#moldesign-content').append("") | + | $('#moldesign-content').append("<h1>Reader</h1><p>After storing a signal through our writer module, the occurrence of DNA methylation still needs to be detected. We call this process the reader.</p><p>In prokaryotes it is understood that DNA methylation is used to protect endogenous DNA from restriction by endogenous restriction enzymes (RE). The M.ScaI methyltransferase is able to methylate a specific DNA sequence which can be recognized by the ScaI restriction enzyme. Addition of a methyl group to the 5th cytosine of this sequence inhibits the binding of ScaI restriction enzyme and cutting of the DNA at this specific recognition site.</p><p>To make a complete reader module an interface for the signal to be stored and retrieved was required. For this purpose the reader module (BBa_K874040) was created comprising of the consensus ScaI restriction site surrounded by two Polydactyl Zinc Finger binding sites from the writer module.</p><p>The information stored in the Cellular Logbook and can be retrieved as described in the “Experimental Results”. Following plasmid isolation and restriction digestion using the ScaI restriction enzyme, the methylation status of the Cellular Logbook can be inferred according to the restriction profile visualized on gel electrophoresis.</p>") |
} | } | ||
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Revision as of 00:26, 27 September 2012
