Team:Amsterdam/project/diary/lab

From 2012.igem.org

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<u><h4>26/04/2012</h4></u>
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<u><center><h4>26/04/2012</h4></center></u>
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We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months.
We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months.
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We will do this by transforming an GFP into an pET28 vector using our transformation protocol.
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We will do this by cloning a GFP into a pET28 vector using our transformation protocol.
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27/04/2012
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<u><center><h4>27/04/2012</h4></center></u>
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Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix.
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<u><center><h4>07/05/2012</h4></center></u>
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Preparing O/N cultures which can be taken out of 37C in the weekend by a friend
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<u><center><h4>08/05/2012</h4></center></u>
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Our friend former igem member Yuri of 2011 will take out our O/N cultures.
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<u><center><h4>09/05/2012</h4></center></u>
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Yuri forgot... Start again. Note; rely on ourselves
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<u><center><h4>10/05/2012</h4></center></u>
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The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture.
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Gel:
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Starting our first PCR.
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{{Team:Amsterdam/Foot}}
{{Team:Amsterdam/Foot}}

Revision as of 13:48, 3 August 2012

Contents

Lab Journal

26/04/2012

We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. We will do this by cloning a GFP into a pET28 vector using our transformation protocol.

27/04/2012

Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix.

07/05/2012

Preparing O/N cultures which can be taken out of 37C in the weekend by a friend

08/05/2012

Our friend former igem member Yuri of 2011 will take out our O/N cultures.

09/05/2012

Yuri forgot... Start again. Note; rely on ourselves

10/05/2012

The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. Gel: Starting our first PCR.



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