Team:Amsterdam/project/characterization experiments/

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During the [growth curve experiment] several samples were taken on different time points to investigate the occurrence of methylation over the progression of time.
During the [growth curve experiment] several samples were taken on different time points to investigate the occurrence of methylation over the progression of time.
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[[File:Amsterdam_characterization_experiments_gel_growth_curve_1.png|300px]]
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[[File:Amsterdam_characterization_experiments_gel_growth_curve_1.png|300px|right]]
Figure X shows first the samples without and then the samples with induction reveals an intermediate restriction profile over the progression of time with a gradual shift toward cut, without methylation, plasmid. But the two samples taken after 24 hours show a difference. The sample with signal shows only uncut, fully methylated, plasmid.
Figure X shows first the samples without and then the samples with induction reveals an intermediate restriction profile over the progression of time with a gradual shift toward cut, without methylation, plasmid. But the two samples taken after 24 hours show a difference. The sample with signal shows only uncut, fully methylated, plasmid.
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<h4>48 hour exposure/ Log vs Stationary</h4>
<h4>48 hour exposure/ Log vs Stationary</h4>
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As with the LacH we suspect that the construct will act differently when taken from a stationary culture. So two experiments were performed on construct pSB1AT3+pBAD+Mtase where bacteria were grown for a minimum of 24 hours to reach stationary phase in the presence of arabinose.
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[[File:Amsterdam_characterization_experiments_gel_48_hour_exposure_log_vs_stationary_1.png|300px|right]]
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[[File:Amsterdam_characterization_experiments_gel_48_hour_exposure_log_vs_stationary_2.png|300px|right]]
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[[File:Amsterdam_characterization_experiments_gel_48_hour_exposure_log_vs_stationary_2.png|300px]]
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As with the LacH we suspect that the construct will act differently when taken from a stationary culture. So two experiments were performed on construct pSB1AT3+pBAD+Mtase where bacteria were grown for a minimum of 24 hours to reach stationary phase in the presence of arabinose.
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[[File:Amsterdam_characterization_experiments_gel_48_hour_exposure_log_vs_stationary_1.png|300px]]
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Both figures X and Y show a dramatic shift from the intermediate digestion profile to the ‘on’ digestion profile. This is considered as a great success.
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Both figures X and Y show a dramatic shift from the intermediate digestion profile to the ‘on’ digestion profile. This is considered as a great success.<br\><br\><br\><br\>
<h4>Growth curve digestion</h4>
<h4>Growth curve digestion</h4>

Latest revision as of 03:31, 26 September 2012