Team:Amsterdam/practices/results

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<h1>Human Practices: Results</h1>
<h1>Human Practices: Results</h1>
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__NOTOC__
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<h4>Social scientist</h4>
<h4>Social scientist</h4>
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<h4>Experts in biotechnology</h4>
<h4>Experts in biotechnology</h4>
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[[File:Amsterdam_practices_1.jpg|300px|right|thumb|Figure 1: Presenting our project in an early stage to the experts at the UvA]]
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[[File:Amsterdam_practices_1.jpg|300px|right|thumb|Presenting our project in an early stage to the experts at the UvA]]
In the first month of our project we organized presentations at both universities (UvA and VU) and invited scientists from the departments of [http://www.falw.vu.nl/nl/onderzoek/molecular-cell-biology/ ''Molecular Cell Biology''] and the [http://sils.uva.nl/ ''Swammerdam Institute for Life Sciences (SILS)'']. During these presentations we explained how we wanted to realize our project, the Cellular Logbook. We proposed our designed [https://2012.igem.org/Team:Amsterdam/project/molecular_design ''molecular mechanisms''] of the Cellular Logbook to these experts. Although most feedback confirmed the neatness of our project design, we received some relevant feedback:
In the first month of our project we organized presentations at both universities (UvA and VU) and invited scientists from the departments of [http://www.falw.vu.nl/nl/onderzoek/molecular-cell-biology/ ''Molecular Cell Biology''] and the [http://sils.uva.nl/ ''Swammerdam Institute for Life Sciences (SILS)'']. During these presentations we explained how we wanted to realize our project, the Cellular Logbook. We proposed our designed [https://2012.igem.org/Team:Amsterdam/project/molecular_design ''molecular mechanisms''] of the Cellular Logbook to these experts. Although most feedback confirmed the neatness of our project design, we received some relevant feedback:
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'''Problems envisioned'''<br\>
'''Problems envisioned'''<br\>
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* Release of GMOs into the environment
* Release of GMOs into the environment
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<h5>Waternet (Amsterdam, the Netherlands)</h5>
<h5>Waternet (Amsterdam, the Netherlands)</h5>
Ron van der Oost is a toxicologist at a Dutch water company, named [https://www.waternet.nl/ ''Waternet'']. He is specialized in research on risks, effects and behavior of emerging substances in the water cycle. Waternet is the only company in the Netherlands that focuses on the whole water cycle. Waternet is responsible for cleaning wastewater, making water drinkable and monitor and clean surface water.
Ron van der Oost is a toxicologist at a Dutch water company, named [https://www.waternet.nl/ ''Waternet'']. He is specialized in research on risks, effects and behavior of emerging substances in the water cycle. Waternet is the only company in the Netherlands that focuses on the whole water cycle. Waternet is responsible for cleaning wastewater, making water drinkable and monitor and clean surface water.
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[[File:Amsterdam_practices_2.jpg|300px|right|thumbs|Figure 2: Ron van Oost from waternet discussing possible project applications]]
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[[File:Amsterdam_practices_2.jpg|300px|right|thumbs|Ron van Oost from waternet discussing possible project applications]]
Ron van der Oost was really interested in our multi-sensor idea. Right now they use 20 different sensors for different groups of compounds. These bioassays are not optimal. They are really expensive and it is hard to normalize the data (at what point does a certain concentration become toxic?). Therefore it is often necessary to get a toxicologist to look at the data from bioassays, what makes it even more expensive. He pointed out some crucial issues regarding the potential use of our Cellular Logbook:
Ron van der Oost was really interested in our multi-sensor idea. Right now they use 20 different sensors for different groups of compounds. These bioassays are not optimal. They are really expensive and it is hard to normalize the data (at what point does a certain concentration become toxic?). Therefore it is often necessary to get a toxicologist to look at the data from bioassays, what makes it even more expensive. He pointed out some crucial issues regarding the potential use of our Cellular Logbook:
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* The sensor should be able to monitor it’s environment for quit some time; the system needs to be ‘up and running’; a lot of systems they use now can be in the water for weeks/months. The time-indicator is an interesting feature -> let the system be in the water for 4 weeks and register when a certain compound was registered.
* The sensor should be able to monitor it’s environment for quit some time; the system needs to be ‘up and running’; a lot of systems they use now can be in the water for weeks/months. The time-indicator is an interesting feature -> let the system be in the water for 4 weeks and register when a certain compound was registered.
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* What (groups of) compounds can we measure?
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* What groups of compounds can we measure?
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* Maybe most important: how do you want to put the GMO’s in the water? By using some sort of filter/membrane, where the bacteria can’t escape from, but can sense their environment. He mentioned passive sampling/samplers.
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* Maybe most important: how do you want to put the GMO’s in the water? By using some sort of filter, where the bacteria can’t escape from, but can sense their environment. He mentioned passive sampling.
'''Points of action'''
'''Points of action'''
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* Check which (20) compounds we could detect
* Check which (20) compounds we could detect
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<h4>Biosafety Officer</h4>
<h4>Biosafety Officer</h4>
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Some safety and security questions were already addressed during our talks with scientist and companies. But for a more extensive and fundamental way of addressing these kinds of issues, we contacted dr. Cécile van der Vlugt, from the National Institute of Public Health and the Environment (RIVM). She is specialized in the risk assessment of Genetically Modified Organisms (GMOs). We were interested in what she would think about a possible application and thereby controlled release of our Cellular Logbook into the environment.
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Some safety and security questions were already addressed during our talks with scientist and companies. But for a more extensive and fundamental way of addressing these kinds of issues, we contacted dr. Cécile van der Vlugt, from the [http://www.rivm.nl/ ''National Institute of Public Health and the Environment (RIVM)'']. She is specialized in the risk assessment of Genetically Modified Organisms (GMOs). We were interested in what she would think about a possible application and thereby controlled release of our Cellular Logbook into the environment.
'''Biosafety'''<br\>
'''Biosafety'''<br\>
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'''Points of action'''<br\>
'''Points of action'''<br\>
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Since our Cellular Logbook system contains genes of which there products have a role in genome rearrangements (the M.ScaI methyltranserase and a Zinc Finger), we included a little risk assessment (link to 2nd question in safety) in the design process of our Cellular Logbook.
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Since our Cellular Logbook system contains genes of which there products have a role in genome rearrangements (the M.ScaI methyltranserase and a Zinc Finger), we included a little [https://2012.igem.org/Team:Amsterdam/safety/questions ''risk assessment''] in the design process of our Cellular Logbook.
<h4>Human Outreach</h4>
<h4>Human Outreach</h4>
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We had two main goals concerning public engagement and human outreach:
We had two main goals concerning public engagement and human outreach:
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'''1. Introducing SB to the general public and engaging in a dialogue.'''
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'''1. Introducing SB to the general public and engaging in a dialogue.'''<br\>
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We approached a broad audience by:
We approached a broad audience by:
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* Featuring in a documentary that will be broadcasted next year in Germany, Belgium and the Netherlands.
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* Featuring in a [https://2012.igem.org/Team:Amsterdam/practices/results#Documentary ''documentary''] that will be broadcasted next year in Germany, Belgium and the Netherlands.
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* Participating on a national art, music and science festival, named Discovery Festival.
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'''2. Creating awareness amongst students of the two universities of Amsterdam (UvA and VU).'''
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* Participating on a national art, music and science festival, named [https://2012.igem.org/Team:Amsterdam/practices/results#Discovery_Festival ''Discovery Festival''].
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'''2. Creating awareness amongst students of the two universities of Amsterdam (UvA and VU).'''<br\>
Young, bright minded scientist earn to get acquainted with the exciting and promising field of SB. Especially, since we have noticed that only a little group of students in Amsterdam ever heard about SB and iGEM, we felt called upon to change this. We decided to do this in two ways:
Young, bright minded scientist earn to get acquainted with the exciting and promising field of SB. Especially, since we have noticed that only a little group of students in Amsterdam ever heard about SB and iGEM, we felt called upon to change this. We decided to do this in two ways:
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<h5>National iGEM Collaboration</h5>
<h5>National iGEM Collaboration</h5>
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[[File:Amsterdam_practices_3.jpg|300px|right|thumb|Meeting with the other Dutch teams to discuss our representation at the Discovery Festival]]
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[[File:Amsterdam_practices_3.jpg|300px|right|thumb|Meeting with the other Dutch teams to discuss the program of the Discovery Festival]]
We collaborated with the iGEM teams from Eindhoven, Wageningen and Groningen to make iGEM and SB feature in the program of Discovery Festival. During the summer we had several meetings with representatives from each collaborating iGEM team. During these sessions we discussed, together with the organization of the event, on how to incorporate SB into the program. All teams took their responsibility by providing and preparing part of the program. In addition, we divided all participating teams over the three locations, so that iGEM would be represented on all locations. Because of the excellent teamwork and effort, we were able to realize the following program:
We collaborated with the iGEM teams from Eindhoven, Wageningen and Groningen to make iGEM and SB feature in the program of Discovery Festival. During the summer we had several meetings with representatives from each collaborating iGEM team. During these sessions we discussed, together with the organization of the event, on how to incorporate SB into the program. All teams took their responsibility by providing and preparing part of the program. In addition, we divided all participating teams over the three locations, so that iGEM would be represented on all locations. Because of the excellent teamwork and effort, we were able to realize the following program:
'''The program'''
'''The program'''
[[File:Amsterdam_practices_4.jpg|300px|thumb|Flyer for the Discovery Festival]]
[[File:Amsterdam_practices_4.jpg|300px|thumb|Flyer for the Discovery Festival]]
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'''1. The SB-experience'''
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'''1. The SB-experience'''<br\>
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As main part of the program, people will experience the process of genetically modifying bacteria by following a 7-step workshop in an improvised laboratorial setting: From ordering DNA online, to implementing the perceivable characteristic into bacteria and showing the resulting bacteria on agar plate. Safety is of course a priority: no actual GMOs or E.coli will be used.
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As main part of the program, people will experience the process of
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genetically modifying bacteria by following a 7-step workshop in an improvised laboratorial setting: From ordering DNA online, to implementing the perceivable characteristic into bacteria and showing the resulting bacteria on agar plate. Safety is of course a priority: no actual GMOs or E.coli will be used.
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'''2. The posters'''
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'''2. The posters'''<br\>
We have made a wall full of posters, on which several fictional and non-fictional iGEM-projects are shown. The goal of this is to give the people an idea of the potential of SB, but also to inspire them and see how they will react on this.
We have made a wall full of posters, on which several fictional and non-fictional iGEM-projects are shown. The goal of this is to give the people an idea of the potential of SB, but also to inspire them and see how they will react on this.
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'''3. What would you do with your own iGEM project?'''
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'''3. What would you do with your own iGEM project?'''<br\>
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After the experience, people are asked what they would do if they could start their own iGEM project. All these ideas are recorded on a video guestbook.
After the experience, people are asked what they would do if they could start their own iGEM project. All these ideas are recorded on a video guestbook.
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'''4. Tuur van Balen'''
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'''4. Tuur van Balen'''<br\>
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We invited [http://www.tuurvanbalen.com/ ''Tuur van Balen''], an artist who got heavily inspired by SB, to the Discovery Festival in Amsterdam. His work will prove to be a stimulus in introducing SB to the public.
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We invited Tuur van Balen, an artist who got heavily inspired by SB, to the Discovery Festival in Amsterdam. His work will prove to be a stimulus in introducing SB to the public.
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Because the event will take place after the wiki-freeze we are unable to show the results. On this link (link to discovery facebook?) you can see a report of the festival!
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Because the event will take place after the wiki-freeze we are unable to show the results. Click [http://www.facebook.com/IgemAmsterdam2012 ''here''] to see a report of the festival!
<h5>Documentary</h5>
<h5>Documentary</h5>
[[File:Amsterdam_practices_5.jpg|300px|right|thumb|Frank Theys, the documentary maker]]
[[File:Amsterdam_practices_5.jpg|300px|right|thumb|Frank Theys, the documentary maker]]
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Together with the iGEM team from Delft, the Netherlands Institute for Neuroscience (NIN) and the Advanced Nano Characterization Center (ANCC) from Japan, we will feature in a documentary called Lab Life, by Belgian documentary maker Frank Theys. The documentary will show a portrait of the daily life around innovative scientific research projects. Moreover, the documentary will focus on responsible innovation by showing the projects through the perspective of a social scientist that follows these projects. By highlighting the aspects of CTA and MM, this documentary is the perfect extension of our Interactive iGEM research approach. Lab Life will be broadcasted next year on German, Belgian and Dutch television.
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Together with the iGEM team from Delft, the Netherlands Institute for Neuroscience (NIN) and the Advanced Nano Characterization Center (ANCC) from Japan, we will feature in a documentary called [http://www.savagefilm.be/documentary/LAB-LIFE ''Lab Life''], by Belgian documentary maker Frank Theys. The documentary will show a portrait of the daily life around innovative scientific research projects. Moreover, the documentary will focus on responsible innovation by showing the projects through the perspective of a social scientist that follows these projects. By highlighting the aspects of CTA and MM, this documentary is the perfect extension of our Interactive iGEM research approach. Lab Life will be broadcasted next year on German, Belgian and Dutch television.
<h5>Press releases</h5>
<h5>Press releases</h5>
'''Press releases in magazines from UvA and VU'''
'''Press releases in magazines from UvA and VU'''
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VU: http://www.advalvas.vu.nl/nieuws/op-naar-het-wk-synthetische-biologie
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VU: [http://www.advalvas.vu.nl/nieuws/op-naar-het-wk-synthetische-biologie ''Ad Valvas'']
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UvA: Unfortunately not published yet.
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UvA: Folia. Unfortunately not published yet.
<h1>References</h1>
<h1>References</h1>

Latest revision as of 03:55, 27 September 2012