Team:Amsterdam/extra/protocols

From 2012.igem.org

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*From all 8 cultures take 1.5 ml sample and put on ice (-20 C)  these are negative control reference points (IPTG- and ARA-)
*From all 8 cultures take 1.5 ml sample and put on ice (-20 C)  these are negative control reference points (IPTG- and ARA-)
*To 3 of 4 cultures) (day 2 – step 5) each add 100mM IPTG, these will become the IPTGex30, IPTGex60, IPTGex120 cultures
*To 3 of 4 cultures) (day 2 – step 5) each add 100mM IPTG, these will become the IPTGex30, IPTGex60, IPTGex120 cultures
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*To 3 of 4 cultures (day 2 – step 6) each add excessive amount of Arabinose (need to ask Glenn how much he used), these will become the ARAex30, ARAex60, ARAex120 cultures
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*To 3 of 4 cultures (day 2 – step 6) each add excessive amount of Arabinose, these will become the ARAex30, ARAex60, ARAex120 cultures
*From all 8 cultures (IPTG-, IPTGex30, IPTGex60, IPTGex120, ARA-, ARAex30, ARAex60, ARAex120) take 1.5 ml sample and put on ice (-20 C) (IPTG t0 & ARA t0)
*From all 8 cultures (IPTG-, IPTGex30, IPTGex60, IPTGex120, ARA-, ARAex30, ARAex60, ARAex120) take 1.5 ml sample and put on ice (-20 C) (IPTG t0 & ARA t0)
*At 30 min take for all 8 cultures (IPTG-, IPTGex30, IPTGex60, IPTGex120, ARA-, ARAex30, ARAex60, ARAex120) a 1.5 ml sample and put on ice (-20 C)  (IPTG t30 & ARA t30)
*At 30 min take for all 8 cultures (IPTG-, IPTGex30, IPTGex60, IPTGex120, ARA-, ARAex30, ARAex60, ARAex120) a 1.5 ml sample and put on ice (-20 C)  (IPTG t30 & ARA t30)

Revision as of 03:39, 27 September 2012