Team:Amsterdam/extra/diary

From 2012.igem.org

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Digestion of extracted plasmid with NotI and BglII (double digest).
Digestion of extracted plasmid with NotI and BglII (double digest).
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PICTUREEEEEEE
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[[File:Amsterdam_lab_diary_1.jpg|300px|border]]
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Pooled all the PCR products for peGFP and gel extract the correct band. Elution in 25µl miliQ water.
Pooled all the PCR products for peGFP and gel extract the correct band. Elution in 25µl miliQ water.
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PICTUUUUREEEE
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[[File:Amsterdam_lab_diary_2.jpg|300px|border]]
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Gel electrophoresis of precipitated DNA pET28a+ from 25/05/2012
Gel electrophoresis of precipitated DNA pET28a+ from 25/05/2012
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PICTUUUURE
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[[File:Amsterdam_lab_diary_3.jpg|300px|border]]
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PCR pET28a+ -> annealing temperature 62˚C.
PCR pET28a+ -> annealing temperature 62˚C.
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PICTUUUUUREEE
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[[File:Amsterdam_lab_diary_4.jpg|300px|border]]
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Repeat what was done on 14/05 (PCR Temp 60˚C + gel extraction.
Repeat what was done on 14/05 (PCR Temp 60˚C + gel extraction.
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PICTUUUUURE
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[[File:Amsterdam_lab_diary_5.jpg|300px|border]]
Two products obtained and concentration seems too low. Pfffffff.... Repeat again......And this is only a test, hope it will work better for the actual experiments
Two products obtained and concentration seems too low. Pfffffff.... Repeat again......And this is only a test, hope it will work better for the actual experiments
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<h4>16/05/2012</h4>
<h4>16/05/2012</h4>
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[[File:Amsterdam_lab_diary_6.jpg|300px|border]]
pET28a+ (gel extracted)
pET28a+ (gel extracted)
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PCR of pET28a+ and peGFP -> annealing temperature 56˚C. non-specific DNA products obtained for the vector. Increase annealing temperature? Gel extract?
PCR of pET28a+ and peGFP -> annealing temperature 56˚C. non-specific DNA products obtained for the vector. Increase annealing temperature? Gel extract?
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PIIICTUUUUUREEEE
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[[File:Amsterdam_lab_diary_7.jpg|300px|border]]
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Gel electrophoresis of the PCR reaction performed on 10/05/2012. Non-specific products obtained. Repeat PCR? Change annealing temperature?
Gel electrophoresis of the PCR reaction performed on 10/05/2012. Non-specific products obtained. Repeat PCR? Change annealing temperature?
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PICTUUUUUUREEEE
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[[File:Amsterdam_lab_diary_8.jpg|300px|border]]
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Revision as of 11:24, 26 September 2012