Team:Amsterdam/achievements/experimental

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<h1>Characterization of Experiments</h1>
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<h1>Experimental results</h1>
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<h2>First proof of concept: LacH+Mtase experiments</h2>
<h2>First proof of concept: LacH+Mtase experiments</h2>
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Upon finishing the insertion of our synthesized Methyltransferase under the control of the LacH promoter in the pSB1AT3 backbone, we were able to start testing our first biobrick and first proof of concept.
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We started testing our first biobrick and first proof of concept, upon finishing the insertion of our synthesized Methyltransferase (MTase) under the control of the LacH promoter in the pSB1AT3 backbone (link),
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<h4>Read-out</h4>
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<h4>Setup of the read t</h4>
Since there is one ScaI site residing in the pSB1AT3 backbone and we created one ScaI site via a scar inside our memory module there are different possible restriction profiles to expect. Knowing that the LacH promoter has some basal activity, it is in our line of expectation to observe either an off or intermediate state.
Since there is one ScaI site residing in the pSB1AT3 backbone and we created one ScaI site via a scar inside our memory module there are different possible restriction profiles to expect. Knowing that the LacH promoter has some basal activity, it is in our line of expectation to observe either an off or intermediate state.

Revision as of 12:46, 26 September 2012