Team/CINVESTAV-IPN-UNAM MX/perspectives.htm

From 2012.igem.org

(Difference between revisions)
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     </p>
     </p>
-
<p>Clostridium acetobutylicum is an anaerobic, spore-forming bacterium with the ability to ferment
+
<p><em>Clostridium acetobutylicum</em> is an anaerobic, spore-forming bacterium with the ability to ferment
starch and sugars into solvents. In the past, it has been used for industrial production of acetone and
starch and sugars into solvents. In the past, it has been used for industrial production of acetone and
butanol, until cheap crude oil rendered petrochemical synthesis more economically feasible. Both economic
butanol, until cheap crude oil rendered petrochemical synthesis more economically feasible. Both economic
and environmental aspects have caused the pendulum to swing back again. Molecular biology has allowed
and environmental aspects have caused the pendulum to swing back again. Molecular biology has allowed
  a detailed understanding of genes and enzymes, required for solventogenesis.(1)
  a detailed understanding of genes and enzymes, required for solventogenesis.(1)
-
Taking advantage of the metabolic versatility of R. sphearoides and R. palustris and their ability to fix
+
Taking advantage of the metabolic versatility of <em>R. sphearoides</em> and <em>R. palustris</em> and their ability to fix
Carbon dioxide, we aim to introduce the following constructions into these both bacteria so that the fixed
Carbon dioxide, we aim to introduce the following constructions into these both bacteria so that the fixed
  carbon dioxide becomes into butanol.</p>
  carbon dioxide becomes into butanol.</p>
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  </div>
  </div>
  <p><br>
  <p><br>
-
(A) the regulation part of the Repressor/Antirepressor system linked with the ETFA (electron-
+
(A) The regulation part of the Repressor/Antirepressor system linked with the ETFA (electron-
transfer-flavoprotein, alpha polypeptide) and the ETFB (electron-transfer-flavoprotein, beta
transfer-flavoprotein, alpha polypeptide) and the ETFB (electron-transfer-flavoprotein, beta
  polypeptide) (ETFA/B).</p>
  polypeptide) (ETFA/B).</p>
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  </div>
  </div>
  <p><br>
  <p><br>
-
(B) this biobrick is the production part of the complete
+
(B) This biobrick is the production part of the complete
  Repressor/Antirepressor system, consists in the PpsR dependent promoter sequence liked with the
  Repressor/Antirepressor system, consists in the PpsR dependent promoter sequence liked with the
  butanol production pathway.</p>  
  butanol production pathway.</p>  
  <div align="center"><img src="http://2012.igem.org/wiki/images/b/b7/PerC.jpg" alt="perc" width="763" height="165"></div>
  <div align="center"><img src="http://2012.igem.org/wiki/images/b/b7/PerC.jpg" alt="perc" width="763" height="165"></div>
  <p><br>
  <p><br>
-
(C) the regulation part of the two component system PrrABC linked
+
(C) The regulation part of the two component system PrrABC linked
  with ETFA/B.</p>  
  with ETFA/B.</p>  
  <div align="center"><img src="http://2012.igem.org/wiki/images/1/12/PerD.jpg" width="764" height="142">
  <div align="center"><img src="http://2012.igem.org/wiki/images/1/12/PerD.jpg" width="764" height="142">
  </div>
  </div>
-
  <p>(D) this biobrick is the production part of the complete Two component PrrABC
+
  <p>(D) This biobrick is the production part of the complete Two component PrrABC
  system, consists in the PrrA dependent promoter sequence liked with the butanol production
  system, consists in the PrrA dependent promoter sequence liked with the butanol production
  pathway. </p>
  pathway. </p>
  <div align="center"><img src="http://2012.igem.org/wiki/images/d/d0/PerE.jpg" width="770" height="180"></div>
  <div align="center"><img src="http://2012.igem.org/wiki/images/d/d0/PerE.jpg" width="770" height="180"></div>
-
  <p>(E) The PrrABC-ETFA/B system linked with the butanol production pathway.</p>
+
  <p>(E) The PrrABC-ETFA/B system linked with the PrrA dependent promoter and the butanol production pathway.</p>
-
  <p> We have to clone these constructions into the pRK415 vector, and conjugate R. sphearoides and R. palutris,
+
  <p> We have to clone these constructions into the pRK415 vector, and conjugate <em>R. sphearoides</em> and <em>R. palutris</em>,
  to finally test our system and the butanol production.</p>
  to finally test our system and the butanol production.</p>
  <hr>
  <hr>
-
  <p id="refe">References
+
 
-
1.- Ann N Y Acad ‘Sci., Dürre P., Fermentative butanol production: bulk chemical and biofuel.
+
  <p id="refe">References<br>
 +
 
 +
1.- Ann N Y Acad ‘Sci., Dürre P., <strong>Fermentative butanol production: bulk chemical and biofuel.</strong>
  (2008) Mar;1125:353-62.<br>
  (2008) Mar;1125:353-62.<br>
-
2.- Cong T. Trinh, Elucidating and reprogramming Escherichia coli metabolisms for obligate
+
2.- Cong T. Trinh, <strong>Elucidating and reprogramming <em>Escherichia coli</em> metabolisms for obligate anaerobic n-butanol and isobutanol production</strong> Appl Microbiol Biotechnol DOI 10.1007/s00253-012-
-
anaerobic n-butanol and isobutanol production Appl Microbiol Biotechnol DOI 10.1007/s00253-012-
+
4197-7<br>
4197-7<br>
-
3.- Masayuki Inui & Masako Suda & Sakurako Kimura & Kaori Yasuda & Hiroaki Suzuki & Hiroshi Toda &
+
3.- Masayuki Inui & Masako Suda & Sakurako Kimura & Kaori Yasuda & Hiroaki Suzuki & Hiroshi Toda & Shogo Yamamoto & Shohei Okino & Nobuaki Suzuki & Hideaki Yukawa <strong>Expression of <em>Clostridium acetobutylicum</em> butanol synthetic genes in <em>Escherichia coli</em></strong> Appl Microbiol Biotechnol (2008)
-
Shogo Yamamoto & Shohei Okino & Nobuaki Suzuki & Hideaki Yukawa Expression of Clostridium
+
-
acetobutylicum butanol synthetic genes in Escherichia coli Appl Microbiol Biotechnol (2008)
+
  77:1305–1316<br>
  77:1305–1316<br>
-
4.- Wolfram Andersch, Hubert Bahl, and Gerhard Gottschalk Level of Enzymes Involved in Acetate,
+
4.- Wolfram Andersch, Hubert Bahl, and Gerhard Gottschalk <strong>Level of Enzymes Involved in Acetate, Butyrate, Acetone and Butanol Formation by <em>Clostridium acetobutylicum</em></strong> Eur J Appl Microbiol
-
Butyrate, Acetone and Butanol Formation by Clostridium acetobutylicum Eur J Appl Microbiol
+
  Biotechnol (1983) 18:327-332<br>
  Biotechnol (1983) 18:327-332<br>
-
  5.- Hubert Bahl, Wolfram Andersch, and Gerhard Gottschalk, Continuous
+
  5.- Hubert Bahl, Wolfram Andersch, and Gerhard Gottschalk, <strong>Continuous
-
Production of Acetone and Butanol by Clostridium acetobutylicum in a
+
Production of Acetone and Butanol by <em>Clostridium acetobutylicum</em> in a Two-Stage Phosphate Limited Chemostat</strong> European J Appl Microbiol Biotechnol (1982) 15:201-205</p>
-
Two-Stage Phosphate Limited Chemostat European J Appl Microbiol Biotechnol (1982) 15:201-205</p>
+
   </div>
   </div>
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<div id="piedepagina">
<div id="piedepagina">
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   <p align="center"> Rhodofactory 2012 </p>
+
   <p align="center"> <strong>Rhodofactory</strong> 2012 </p>
<div id="sponsors">
<div id="sponsors">
  <div align="center"><img src="http://2012.igem.org/wiki/images/8/8a/Icytdf.png" alt="icytdf" width="90" height="82" /></div>
  <div align="center"><img src="http://2012.igem.org/wiki/images/8/8a/Icytdf.png" alt="icytdf" width="90" height="82" /></div>

Revision as of 03:58, 26 October 2012

Rho

Perspectives!

With the valuable collaboration of the team iGEM-Alberta 2008, we linked the light-oxygen dependent regulation systems with the butanol production pathway and its electron-transfer-flavoprotein alpha and betha polypeptides, these constructs were donated by Dr. Douglas Ridgway.
(Reference: http://2008.igem.org/Team:The_University_of_Alberta/Butanerd)

Clostridium acetobutylicum is an anaerobic, spore-forming bacterium with the ability to ferment starch and sugars into solvents. In the past, it has been used for industrial production of acetone and butanol, until cheap crude oil rendered petrochemical synthesis more economically feasible. Both economic and environmental aspects have caused the pendulum to swing back again. Molecular biology has allowed a detailed understanding of genes and enzymes, required for solventogenesis.(1) Taking advantage of the metabolic versatility of R. sphearoides and R. palustris and their ability to fix Carbon dioxide, we aim to introduce the following constructions into these both bacteria so that the fixed carbon dioxide becomes into butanol.

We constructed the following circuits:

perA


(A) The regulation part of the Repressor/Antirepressor system linked with the ETFA (electron- transfer-flavoprotein, alpha polypeptide) and the ETFB (electron-transfer-flavoprotein, beta polypeptide) (ETFA/B).

perB


(B) This biobrick is the production part of the complete Repressor/Antirepressor system, consists in the PpsR dependent promoter sequence liked with the butanol production pathway.

perc


(C) The regulation part of the two component system PrrABC linked with ETFA/B.

(D) This biobrick is the production part of the complete Two component PrrABC system, consists in the PrrA dependent promoter sequence liked with the butanol production pathway.

(E) The PrrABC-ETFA/B system linked with the PrrA dependent promoter and the butanol production pathway.

We have to clone these constructions into the pRK415 vector, and conjugate R. sphearoides and R. palutris, to finally test our system and the butanol production.


References
1.- Ann N Y Acad ‘Sci., Dürre P., Fermentative butanol production: bulk chemical and biofuel. (2008) Mar;1125:353-62.
2.- Cong T. Trinh, Elucidating and reprogramming Escherichia coli metabolisms for obligate anaerobic n-butanol and isobutanol production Appl Microbiol Biotechnol DOI 10.1007/s00253-012- 4197-7
3.- Masayuki Inui & Masako Suda & Sakurako Kimura & Kaori Yasuda & Hiroaki Suzuki & Hiroshi Toda & Shogo Yamamoto & Shohei Okino & Nobuaki Suzuki & Hideaki Yukawa Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli Appl Microbiol Biotechnol (2008) 77:1305–1316
4.- Wolfram Andersch, Hubert Bahl, and Gerhard Gottschalk Level of Enzymes Involved in Acetate, Butyrate, Acetone and Butanol Formation by Clostridium acetobutylicum Eur J Appl Microbiol Biotechnol (1983) 18:327-332
5.- Hubert Bahl, Wolfram Andersch, and Gerhard Gottschalk, Continuous Production of Acetone and Butanol by Clostridium acetobutylicum in a Two-Stage Phosphate Limited Chemostat European J Appl Microbiol Biotechnol (1982) 15:201-205


Rhodofactory 2012

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