Team/CINVESTAV-IPN-UNAM MX/home.htm

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       <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis cluster regulation. </p>
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       <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis regulation. </p>
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       <p>The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on <em>R. palustris</em> chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion.</p>
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       <p>The first one is a light dependent system controlled by two proteins that works as an antirepresor/repressor mechanism, and the second one is an oxygen dependent two-component system. We tested these devices on <em>R. palustris</em>. We built a genetic circuit that activate or repress GFP expression in response to external conditions.</p>
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       <p> Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals. </p>
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       <p> Once we characterize the functionality of these networks, we want to create a Rhodofactory, to use our systems to control production butanol using simple signals. </p>
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Revision as of 01:22, 21 October 2012

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The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on R. sphaeroides photosynthesis regulation.

The first one is a light dependent system controlled by two proteins that works as an antirepresor/repressor mechanism, and the second one is an oxygen dependent two-component system. We tested these devices on R. palustris. We built a genetic circuit that activate or repress GFP expression in response to external conditions.

Once we characterize the functionality of these networks, we want to create a Rhodofactory, to use our systems to control production butanol using simple signals.

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