Team/CINVESTAV-IPN-UNAM MX/Safety.htm

From 2012.igem.org

(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml"><head>
+
<html xmlns="http://www.w3.org/1999/xhtml">
-
<title>Home</title>
+
<head>
 +
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
 +
<title>Rho</title>
-
<link rel="stylesheet" href="https://dl.dropbox.com/u/199422/Cinvestav/style.css" />
+
<style type="text/css">
 +
#top-section {
 +
position: relative;
 +
height: 20px;
 +
width: 975px;
 +
margin: 0 auto 0 auto;
 +
border-left: hidden;
 +
border-right: hidden;
 +
border-bottom: hidden;
 +
}
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
* {
-
<style type="text/css">td img {display: block;}</style>
+
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
body {
 +
background: #FFFFFF url(https://static.igem.org/mediawiki/2012/f/f3/Fondo_bod.jpg) repeat-x;
 +
text-align: justify;
 +
font-family: Georgia, "Times New Roman", Times, serif;
 +
font-size: 12px;
 +
color: #000000;
 +
}
 +
 
 +
h1, h2, h3 {
 +
color: #333333;
 +
}
 +
 
 +
h1, h2 {
 +
margin-bottom: 20px;
 +
font-weight: normal;
 +
}
 +
 
 +
h1 {
 +
font-size: 197%;
 +
}
 +
 
 +
h2 {
 +
font-size: 152%;
 +
}
 +
 
 +
h3 {
 +
font-size: 100%;
 +
}
 +
 
 +
p, ul, ol {
 +
margin-bottom: 20px;
 +
line-height: 1.8em;
 +
}
 +
 
 +
ul, ol {
 +
margin-left: 3em;
 +
}
 +
 
 +
blockquote {
 +
padding-left: 3em;
 +
background: url(https://static.igem.org/mediawiki/2012/1/11/Img08.gif) no-repeat;
 +
font-style: italic;
 +
}
 +
 
 +
a:link {
 +
color: #1953A7;
 +
}
 +
 
 +
a:active {
 +
color: #ED5C0C;
 +
}
 +
 
 +
a:visited {
 +
color: #666666;
 +
}
 +
 
 +
a:hover {
 +
text-decoration: none;
 +
color: #067600;
 +
}
 +
 
 +
img {
 +
border: none;
 +
}
 +
 
 +
img.left {
 +
float: left;
 +
margin: 0 20px 10px 0;
 +
}
 +
 
 +
/* Menu */
 +
 
 +
#menu {
 +
width: 770px;
 +
height: 65px;
 +
margin: 0 auto;
 +
border-right: 1px solid #EDEBD5;
 +
}
 +
 
 +
#menu ul {
 +
margin: 0;
 +
padding: 0;
 +
list-style: none;
 +
}
 +
 
 +
#menu li {
 +
display: inline;
 +
font-size: 12px;
 +
color:#4E4E4E;
 +
}
 +
 
 +
#menu a {
 +
display: block;
 +
float: left;
 +
width: 95px;
 +
height: 45px;
 +
padding: 10px 0 0 0;
 +
border-left: 1px solid #EDEBD5;
 +
text-transform: lowercase;
 +
text-decoration: none;
 +
text-align: center;
 +
font-size: 135%;
 +
color: #BABABA;
 +
}
 +
 
 +
#menu a:hover {
 +
background: #F7F7F4;
 +
}
 +
 
 +
#menu .active a {
 +
background: #5dbb10;
 +
color: #FFFFFF;
 +
}
 +
 
 +
/* Header */
 +
 
 +
#header {
 +
width: 770px;
 +
height: 170px;
 +
margin: 0 auto;
 +
}
 +
 
 +
#header h1 {
 +
float: left;
 +
width: 220px;
 +
height: 100px;
 +
margin: 0;
 +
padding: 70px 0 0 0;
 +
background: url(https://static.igem.org/mediawiki/2012/0/0a/Img02.jpg) no-repeat;
 +
text-align: center;
 +
font-size: 36px;
 +
}
 +
 
 +
#header h2 {
 +
float: right;
 +
width: 530px;
 +
height: 86px;
 +
margin: 0;
 +
background: url(https://static.igem.org/mediawiki/2012/4/4a/Img03.jpg) no-repeat;
 +
padding: 84px 20px 0 0;
 +
text-align: right;
 +
}
 +
 
 +
#header a {
 +
text-transform: lowercase;
 +
text-decoration: none;
 +
font-style: italic;
 +
font-weight: normal;
 +
color: #FFFFFF;
 +
}
 +
 
 +
/* Page */
 +
 
 +
#page {
 +
width: 770px;
 +
margin: 0 auto;
 +
padding: 20px 0;
 +
}
 +
#context{
 +
float: left;
 +
width: 570px;
 +
padding-top:5px;
 +
}       
 +
/* menudecontenido */
 +
 
 +
#sidebar {
 +
float: right;
 +
width: 160px;
 +
padding: 15px;
 +
background: #EDEBD5;
 +
}
 +
 
 +
#sidebar h2 {
 +
margin: 0;
 +
padding: 5px 15px;
 +
color: #FFFFFF;
 +
}
 +
 
 +
#sidebar h3 {
 +
font-size: 60%;
 +
color: #FFFFFF;
 +
}
 +
 
 +
#sidebar ul {
 +
margin: 0;
 +
padding: 15px;
 +
list-style: none;
 +
}
 +
 
 +
#sidebar li {
 +
              font-size: 12px;
 +
  text-align:left;
 +
}
 +
 
 +
#sidebar a {
 +
color: #FFFFFF;
 +
}
 +
 
 +
.orangebox {
 +
background: url(https://static.igem.org/mediawiki/2012/b/b0/Img05.gif) repeat-y;
 +
}
 +
 
 +
.orangebox h2 {
 +
background: url(https://static.igem.org/mediawiki/2012/c/c2/Img06.gif) repeat-y;
 +
}
 +
 
 +
/* Footer */
 +
 
 +
#piedepagina {
 +
clear: both;
 +
height: 150px;
 +
padding: 25px 0;
 +
background: #EDEBD5 url(https://static.igem.org/mediawiki/2012/a/a5/Img04.gif) repeat-x;
 +
}
 +
 
 +
#sponsors{
 +
 +
float: left;
 +
width: 85px;
 +
padding-top:5px;
 +
background: #EDEBD5;
 +
 +
      }
 +
#text2
 +
{
 +
color: #333333;
 +
font-size: 152%;
 +
margin-bottom: 20px;
 +
font-weight: normal;
 +
}
 +
#refe
 +
{
 +
color: #000000;
 +
font-size:9;
 +
margin-bottom: 20px;
 +
font-weight: normal;
 +
}
 +
 
 +
.firstHeading {
 +
margin-bottom: .1em;
 +
visibility:hidden;
 +
}
 +
 
 +
img[src*="/wiki/skins/common/images/wiki.png"]{
 +
    width:0;
 +
    height:0px;
 +
    padding-right:975px;
 +
    background:transparent url(https://2011.igem.org/wiki/skins/common/images/wiki.png) top left no-repeat;
 +
}
 +
 
 +
</style>
 +
 
 +
<meta name="keywords" content="" />
 +
<meta name="description" content="" />
-
<!--Fireworks CS6 Dreamweaver CS6 target.  Created Tue Sep 25 17:05:18 GMT-0500 (Central Daylight Time (Mexico)) 2012-->
 
</head>
</head>
-
<body bgcolor="#ffffff">
+
<body>
-
<table style="display: inline-table;" border="0" cellpadding="0" cellspacing="0" width="950" bgcolor="">
+
<div id="menu">
-
<!-- fwtable fwsrc="INDEX.fw.png" fwpage="Page 1" fwbase="principal.jpg" fwstyle="Dreamweaver" fwdocid = "927795023" fwnested="0" -->
+
<ul>
-
  <tr>
+
<li><a href="home.htm">Home</a></li>
-
  <td><img src="images/spacer.gif" width="240" height="1" alt="" /></td>
+
<li><a href="rhodofactory.htm">Rhodofactory</a></li>
-
  <td><img src="images/spacer.gif" width="710" height="1" alt="" /></td>
+
<li><a href="promoterselection.htm">Promoter selection </a></li>
-
  <td><img src="images/spacer.gif" width="1" height="1" alt="" /></td>
+
<li><a href="results.htm">results </a></li>
-
  </tr>
+
<li><a href="perspectives.htm">Perspectives</a></li>
 +
<li><a href="Modelling.htm">Modeling</a></li>
 +
<li><a href="aboutus.htm">About us</a> </li>
 +
<li class="active"><a href="outreach.htm">outreach</a></li>
 +
</ul>
 +
</div>
 +
<!-- end #menu -->
 +
<div id="header">
 +
<h1><a href="#"></a></h1>  
 +
<h2><span></span></h2>
 +
</div>
 +
<!-- end #header -->
 +
<div id="page">
 +
<div id="context">
 +
 +
<h1><em>Safety!</em></h1>
 +
            </br>
 +
<h3>Question 1:''Would any of your project ideas raise safety issues in terms of researcher, public or
 +
enviromental safety?'''</h3><br>
-
  <tr>
+
<p>Purple Non sulfur Bacteria are one of the most metabolically diverse groups known in nature, they
-
  <td><img src="https://static.igem.org/mediawiki/2012/6/68/LOGOPRIN.jpg" width="240" height="178" alt="Logo Principal" /></td>
+
are able to grow under different conditions such as, aerobic respiration, anoxigenic
-
  <td bgcolor="01A79D"><ul class="nav">
+
photosynthesis, and anaerobic fermentation. Due to its diverse metabolism and their ability to eat
-
<li>
+
aromatic compounds, these bacteria can live in hostile media such as polluted water. Despite all
-
<a href="/Team/CINVESTAV-IPN-UNAM_MX/home.htm">Home<span class="flecha">&#9660;</span></a>
+
these features, work with these microorganisms in lab conditions is very laborious; especially since
-
</li>
+
its growth medium is a complex mix of metals and vitamins.<br><br>
-
<li>
+
 
-
<a href="#">Rhodofactory<span class="flecha">&#9660;</span></a>
+
 
-
<ul>
+
The risk in PNSB management in the lab is very low since they are non-pathogenic bacteria. <em>R.
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Overview.htm">Overview<span class="flecha">&#9660;</span></a></li>
+
sphaeroides</em> and <em>R. palustris´</em> grow rates are quite slow, thus, our genetic systems do not represent
-
           
+
a public safety menace since they are highly specific. Besides, we have designed these systems for
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Light Response.htm">Light Response<span class="flecha">&#9660;</span></a></li>
+
its specific functioning in purple photosynthetic bacteria. Nevertheless, it is very important to take
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Oxigen Response.htm">Oxigen Response<span class="flecha">&#9660;</span></a></li>
+
special care during our wetlab work, taking into account the basic precautions and methods
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Chassis.htm">Chassis<span class="flecha">&#9660;</span></a></li>
+
commonly used in microbiology.<br><br>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Perspective.htm">Perspective<span class="flecha">&#9660;</span></a></li>
+
 
-
+
 
-
</ul>
+
Our project aims to build two regulation systems, and introduce them into <em>Rhodopseudomonas
-
</li>
+
palustris.</em> Since PCR isolation of the five genes that form the system is extremely difficult, gene
-
<li>
+
synthesis technology provided by Genescript was used instead for the obtainment of these
-
<a href="">Results<span class="flecha">&#9660;</span></a>
+
BioBricks. All our biobricks</p>
-
<ul>
+
<br>
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Biobricks.htm">Biobricks<span class="flecha">&#9660;</span></a></li>
+
<h3> Question 2: '''Do any of the new BioBrick parts (or devices) that you made this year raise any
-
           
+
safety issues?'''</h3><br>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Notebook.htm">Notebook<span class="flecha">&#9660;</span></a></li>
+
 
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Protocol.htm">Protocol<span class="flecha">&#9660;</span></a></li>
+
<p>No, our BioBricks parts came from <em>Rhodobacter sphaeroides,</em> this bacteria is non-pathogenic and it
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/palustris response.htm">R. palustris response<span class="flecha">&#9660;</span></a></li>
+
can be worked in a Biosafety level 1 laboratory, based on the CDC Biosafety in Microbiological and
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/sphaeroides response.htm">R. sphaeroides response<span class="flecha">&#9660;</span></a></li>
+
Biomedical Laboratories (CDC, 2007). The functionality of our parts do not produce any dangerous
-
</ul>
+
compound, we are working in gene expression control. Furthermore, we used BioBricks from iGEM
-
</li>
+
distribution for all our assemblies, for example GFP as reporter, these parts do not represent a
 +
biological risk.</p>
 +
<h3>Question 3: '''Is there a local biosafety group, committee, or review board at your institution? If
 +
no, which specific biosafety rules or guidelines do you have to consider in your country?'''</h3><br>
 +
 
 +
 
 +
<p>No, a Biosafety committee must be integrated, by a multidisciplinary group of members among
 +
Research Centers, Industries and Universities. This committee should provide expert advice on
 +
laboratory, biosafety and biosecurity issues related to research and other academic activities.</p>
 +
<p> The objectives of this group should be: <br>
 +
 
 +
<li>Plan, design and implement the necessary instruments for biosafety risk assessment.<br>
 +
 
 +
<li>Establish, disseminate and analyze safety approaches in handling chemicals, genetically
 +
modified organisms and potentially infectious pathogens that allow us to make decisions
 +
for users in order to minimize potential health risks.<br>
 +
 
 +
<li>Conduct briefings and feedback sessions with the actors involved in biosafety.<br>
 +
<li>Propound consistent Biosecurity regulations, which allow the development and promotion
 +
of basic and applied research.
 +
 +
<p><br>Particular problems should be evaluated case by case for the analysis of their solutions, this
 +
analysis should also include assessment of the risks of alternative technological options for
 +
coping with the specific problem to which the GMO was designed. And finally, it is important
 +
to review other laws; this will help to strengthen biosecurity aspects of other organisms that
 +
are not GMOs.<br><br>
 +
 
 +
 
 +
In Mexico CIBIOGEM, which is a governmental organism, was created to regulate biosafety
 +
issues mainly related with transgenic organisms and GMOs. The mentioned committee should
 +
work along this institution in order to establish a synthetic biology approach to biosafety. </p>
 +
<h3>Question 4: Do you have any other ideas how to deal with safety issues that could be useful for
 +
future iGEM competitions? How could parts, devices and systems be made even safer through
 +
biosafety engineering? </h3><br>
 +
 
 +
 +
<p>As a part of our human practices work, we are trying to implement the Quality Function
 +
Deployment tool in order to answer the most important questions in terms of safety and
 +
biosecurity aplied in Synthetic Biology, also we presented this work in the Fourth International Biosafety and Biosecurity Symposium (SIBB), with great results. The relevance of this work is that through a thorough analysis of the proposed goals
 +
and needs of our work, we are going to be able to make different proposals that support the
 +
development of synthetic biology-based projects in Mexico</p>
 +
 
 +
  </div>
 +
<div id="sidebar">
 +
  <div id="updates" class="orangebox">
 +
<h2>Outreach</h2>
 +
<ul>
 +
<li><a href="Human_Practice.htm" target="_parent">Human Practices</a></li>
 +
<li><a href="Safety.htm">Safety</a></li>
 +
</ul>
-
<li>
+
</div>
-
<a href="#">IGEM Contribution<span class="flecha">&#9660;</span></a>
+
</div>  
-
<ul>
+
  <div style="clear: both;">&nbsp;</div>
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Construction.htm">Construction<span class="flecha">&#9660;</span></a></li>
+
</div>
-
           
+
<!-- end #page -->
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Metods.htm">Metods<span class="flecha">&#9660;</span></a></li>
+
<div id="piedepagina">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Results.htm">Results<span class="flecha">&#9660;</span></a></li>
+
<p align="center"> <strong>Rhodofactory 2012</strong> </p>
-
+
<div id="sponsors">
-
</ul>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width="90" height="82" /></div>
-
</li>
+
</div>
-
<li>
+
<div id="sponsors">
-
<a href="/Team/CINVESTAV-IPN-UNAM_MX/Modelling.htm">Modelling<span class="flecha">&#9660;</span></a>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/6/67/Osli.png" alt="osli" width="88" height="46" /></div>
-
+
</div>
-
</li>  
+
-
   
+
<div id="sponsors">
-
    <li>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width="80" height="97" /></div>
-
<a href="#">Team<span class="flecha">&#9660;</span></a>
+
</div>
-
<ul>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div>
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Members.htm">Members<span class="flecha">&#9660;</span></a></li>
+
<div id="sponsors">
-
           
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="74" height="91" /></div>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Institute.htm">Institute<span class="flecha">&#9660;</span></a></li>
+
</div>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Labs.htm">Labs<span class="flecha">&#9660;</span></a></li>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Sponsor.htm">Sponsor<span class="flecha">&#9660;</span></a></li>
+
<div id="sponsors">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Gallery.htm">Gallery<span class="flecha">&#9660;</span></a></li>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="83" height="93" /></div>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Acknowledgments.htm">Acknowledgments<span class="flecha">&#9660;</span></a></li>
+
-
+
-
</ul>
+
-
</li>
+
-
    <li>
+
-
<a href="#">Outreach<span class="flecha">&#9660;</span></a>
+
-
<ul>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Human Practice.htm">Human Practice<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Safety.htm">Safety<span class="flecha">&#9660;</span></a></li>
+
-
</ul>
+
-
</li>
+
-
   
+
-
   
+
-
</ul></td>
+
-
  <td><img src="images/spacer.gif" width="1" height="150" alt="" /></td>
+
-
  </tr>
+
-
  <tr>
+
-
  <td colspan="2" >
+
-
 
+
-
  <div id="main">
+
-
<div id="bodys_r1_c2">
+
</div>
</div>
-
<div id="bodys_r1_c3">
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div>
-
   
+
    <div id="sponsors">
-
      <img src="https://dl.dropbox.com/u/199422/Cinvestav/images/Safety.fw.png" width="500" height="92" /> </div>
+
      <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="80" height="72" /></div>
-
<div class="clearFloat"></div>
+
-
<div id="bodys_r2_c2"></div>
+
-
<div id="bodys_r2_c3">
+
-
    <div class="Text">
+
-
   
+
-
      <p>&nbsp;</p>
+
-
      <p><strong>Question  1</strong>:<strong>'''Would any of your project ideas raise  safety issues in terms of researcher, public or enviromental safety?'''</strong>      </p>
+
-
      <p><br />
+
-
        Purple Non sulfur Bacteria are one of the most  metabolically diverse groups known in nature, they are able to grow under  different conditions such as, aerobic respiration, anoxigenic photosynthesis,  and anaerobic fermentation. Due to its diverse metabolism and their ability to  eat aromatic compounds, these bacteria can live in hostile media such as  polluted water. Despite all these features, work with these microorganisms in  lab conditions is very laborious; especially since its growth medium is a  complex mix of metals and vitamins.<br />
+
-
        The risk in PNSB management in the lab is very  low since they are non-pathogenic bacteria. R. sphaeroides and R. palustris´  grow rates are quite slow, thus, our genetic systems do not represent a public  safety menace since they are highly specific. Besides, we have designed these  systems for its specific functioning in purple photosynthetic bacteria. Nevertheless,  it is very important to take special care during our wetlab work, taking into  account the basic precautions and methods commonly used in microbiology.<br />
+
-
        Our project aims to build two regulation  systems, and introduce them into Rhodopseudomonas palustris. Since PCR isolation  of the five genes that form the system is extremely difficult, gene synthesis  technology provided by Genescript was used instead for the obtainment of these  BioBricks. All our biobricks </p>
+
-
      <p><br />
+
-
  <strong>Question  2</strong>: <strong>'''Do any of the new BioBrick parts (or  devices) that you made this year raise any safety issues?'''</strong></p>
+
-
      <p><br />
+
-
        No, our BioBricks parts came from Rhodobacter  sphaeroides, this bacteria is non-pathogenic and it can be worked in a  Biosafety level 1 laboratory, based on the CDC Biosafety in Microbiological and  Biomedical Laboratories (CDC, 2007). The functionality of our parts do not  produce any dangerous compound, we are working in gene expression control.  Furthermore, we used BioBricks from iGEM distribution for all our assemblies,  for example GFP as reporter, these parts do not represent a biological risk.</p>
+
-
      <p><br />
+
-
  <strong>Question  3</strong>: <strong>'''Is there a local biosafety group,  committee, or review board at your institution? If no, which specific biosafety  rules or guidelines do you have to consider in your country?'''</strong></p>
+
-
      <p><br />
+
-
        No, a Biosafety committee must be integrated,  by a multidisciplinary group of members among Research Centers, Industries and  Universities. This committee should provide expert advice on laboratory,  biosafety and biosecurity issues related to research and other academic  activities. <br />
+
-
        The objectives of  this group should be:</p>
+
-
      <ul>
+
-
        <li>Plan,  design and implement the necessary instruments for biosafety risk assessment. </li>
+
-
        <li>Establish,  disseminate and analyze safety approaches in handling chemicals, genetically  modified organisms and potentially infectious pathogens that allow us to make decisions  for users in order to minimize potential health risks.</li>
+
-
        <li>Conduct  briefings and feedback sessions with the actors involved in biosafety.</li>
+
-
        <li>Propound  consistent Biosecurity regulations, which allow the development and promotion  of basic and applied research. </li>
+
-
      </ul>
+
-
      <p>Particular problems should be evaluated case by  case for the analysis of their solutions , this analysis should also include  assessment of the risks of alternative technological options for coping with  the specific problem to which the GMO was designed. And finally, it is  important to review other laws; this will help to strengthen biosecurity  aspects of other organisms that are not GMOs.</p>
+
-
      <p>In Mexico CIBIOGEM, which is a governmental  organism, was created to regulate biosafety issues mainly related with transgenic  organisms and GMOs. The mentioned committee should work along this institution  in order to establish a synthetic biology approach to biosafety.</p>
+
-
      <p><strong>Question  4</strong>: <strong>Do you have any other ideas how to deal  with safety issues that could be useful for future iGEM competitions? How could  parts, devices and systems be made even safer through biosafety engineering?</strong></p>
+
-
      <p><br />
+
-
        As a part of our human practices work, we are  trying to implement the Quality Function Deployment tool in order to answer the  most important questions in terms of safety and biosecurity. The relevance of  this work is that through a thorough analysis of the proposed goals and needs  of our work, we are going to be able to make different proposals that support  the development of synthetic biology-based projects in Mexico</p>
+
-
<p align="center">&nbsp;</p>
+
-
            </div>
+
     </div>
     </div>
-
<div class="clearFloat"></div>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div>
 +
<div id="sponsors">
 +
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width="70" height="96" /></div>
 +
</div>
 +
</div>
</div>
-
 
+
<!-- end #footer -->
-
    </td>
+
-
  <td><img src="images/spacer.gif" width="1" height="500" alt="" /></td>
+
-
  </tr>
+
-
</table>
+
</body>
</body>
</html>
</html>

Latest revision as of 05:15, 26 October 2012

Rho

Safety!


Question 1:''Would any of your project ideas raise safety issues in terms of researcher, public or enviromental safety?'''


Purple Non sulfur Bacteria are one of the most metabolically diverse groups known in nature, they are able to grow under different conditions such as, aerobic respiration, anoxigenic photosynthesis, and anaerobic fermentation. Due to its diverse metabolism and their ability to eat aromatic compounds, these bacteria can live in hostile media such as polluted water. Despite all these features, work with these microorganisms in lab conditions is very laborious; especially since its growth medium is a complex mix of metals and vitamins.

The risk in PNSB management in the lab is very low since they are non-pathogenic bacteria. R. sphaeroides and R. palustris´ grow rates are quite slow, thus, our genetic systems do not represent a public safety menace since they are highly specific. Besides, we have designed these systems for its specific functioning in purple photosynthetic bacteria. Nevertheless, it is very important to take special care during our wetlab work, taking into account the basic precautions and methods commonly used in microbiology.

Our project aims to build two regulation systems, and introduce them into Rhodopseudomonas palustris. Since PCR isolation of the five genes that form the system is extremely difficult, gene synthesis technology provided by Genescript was used instead for the obtainment of these BioBricks. All our biobricks


Question 2: '''Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?'''


No, our BioBricks parts came from Rhodobacter sphaeroides, this bacteria is non-pathogenic and it can be worked in a Biosafety level 1 laboratory, based on the CDC Biosafety in Microbiological and Biomedical Laboratories (CDC, 2007). The functionality of our parts do not produce any dangerous compound, we are working in gene expression control. Furthermore, we used BioBricks from iGEM distribution for all our assemblies, for example GFP as reporter, these parts do not represent a biological risk.

Question 3: '''Is there a local biosafety group, committee, or review board at your institution? If no, which specific biosafety rules or guidelines do you have to consider in your country?'''


No, a Biosafety committee must be integrated, by a multidisciplinary group of members among Research Centers, Industries and Universities. This committee should provide expert advice on laboratory, biosafety and biosecurity issues related to research and other academic activities.

The objectives of this group should be:

  • Plan, design and implement the necessary instruments for biosafety risk assessment.
  • Establish, disseminate and analyze safety approaches in handling chemicals, genetically modified organisms and potentially infectious pathogens that allow us to make decisions for users in order to minimize potential health risks.
  • Conduct briefings and feedback sessions with the actors involved in biosafety.
  • Propound consistent Biosecurity regulations, which allow the development and promotion of basic and applied research.


    Particular problems should be evaluated case by case for the analysis of their solutions, this analysis should also include assessment of the risks of alternative technological options for coping with the specific problem to which the GMO was designed. And finally, it is important to review other laws; this will help to strengthen biosecurity aspects of other organisms that are not GMOs.

    In Mexico CIBIOGEM, which is a governmental organism, was created to regulate biosafety issues mainly related with transgenic organisms and GMOs. The mentioned committee should work along this institution in order to establish a synthetic biology approach to biosafety.

    Question 4: Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?


    As a part of our human practices work, we are trying to implement the Quality Function Deployment tool in order to answer the most important questions in terms of safety and biosecurity aplied in Synthetic Biology, also we presented this work in the Fourth International Biosafety and Biosecurity Symposium (SIBB), with great results. The relevance of this work is that through a thorough analysis of the proposed goals and needs of our work, we are going to be able to make different proposals that support the development of synthetic biology-based projects in Mexico

    •  

    Rhodofactory 2012

    icytdf
    osli
    bio
    fermentAS
    cinestav
    genscript
    unam
    gto
    quimica
    valaner
    ipn

    Retrieved from "http://2012.igem.org/Team/CINVESTAV-IPN-UNAM_MX/Safety.htm"