Team/CINVESTAV-IPN-UNAM MX/Overview.htm

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       <p align="justify">The  purple non-sulfur photosynthetic bacteria (PNSB) belong to the  alpha-proteobacteria, because of their genetic regulatory systems which  coordinate different metabolic states, this microorganism are able to grow  under a wide variety of environmental conditions <strong>(1).</strong></p>  
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       <p align="justify">Purple non-sulfur photosynthetic bacteria (PNSB) are metabolically versatile organisms that belong to the  alpha-proteobacteria. This microorganisms are able to grow  under a wide variety of environmental conditions, this is possible due to their sophisticated regulatory systems which coordinate metabolic changes. Our project aims to build two  genetic control systems based on <em>R. sphaeroides</em> photosynthesis  regulation <strong>(1).</strong></p>  
        
        
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       <p>Specifically, our project aims to build two  genetic control systems based on <em>R. sphaeroides</em> photosynthesis cluster  regulation. The first one is the oxygen dependant system PrrA/PrrB, when oxygen tension is high it  remains inactive, and when the oxygen is low it activates gene expression <strong>(2)</strong>. The second system is the light and  oxygen mediated system AppA/PpsR that represses gene expression under aerobic conditions and allows transcription in the absence of oxygen and light <strong>(3)</strong>. </p>
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       <p> The first one is the oxygen dependent system PrrBCA. It activates gene expression when the oxygen is low <strong>(2)</strong>. The second system is the light/oxygen mediated system that strongly represses gene expression under aerobic conditions and allows transcription in the absence of oxygen and light <strong>(3)</strong>. </p>
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         To achieve this goal we designed a genetic  circuit in which GFP expression is oxygen and light-dependent by the  antirepression of PpsR and oxygen dependent by the activation of PrrA/B system.  The lab work is accompanied by a computational model, which will provide a way  of testing our knowledge of these systems. </p>
         To achieve this goal we designed a genetic  circuit in which GFP expression is oxygen and light-dependent by the  antirepression of PpsR and oxygen dependent by the activation of PrrA/B system.  The lab work is accompanied by a computational model, which will provide a way  of testing our knowledge of these systems. </p>
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         Once, we have characterized the  functionality of these regulatory circuits we aim to take advantage of <em>R.  palustris&rsquo; </em>metabolic versatility, and use this bacteria as a microbial factory,  that could work for the production of metabolites with economic value products  using as carbon source such as CO2.</p>
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         Once, we characterize these regulatory circuits we aim to exploit <em>R.  palustris&rsquo; </em>metabolic versatility, and use it as a microbial factory,  that could work for the production economic valuable products  using CO2 as carbon source.</p>
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         We are planning to use the S04147 clostridial butanol production operon (University of Alberta iGEM Team 2007) to  evaluate the synthesis of this biofuel, linking it to our control systems. This  would provide an interesting way to produce butanol using CO2 as  carbon source under anaerobic photosynthetic conditions. </p>
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         We are planning to use the S04147 (University of Alberta iGEM Team 2007) to  evaluate butanol production  controlled by our systems. This  would provide an interesting way to produce this biofuel using photosynthesis under anaerobic conditions. </p>
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       <p><img src="http://2012.igem.org/wiki/images/c/cf/Over1.jpg" width="468" height="456" alt="Figure: General Scheme of the project" /></p>
       <p><img src="http://2012.igem.org/wiki/images/c/cf/Over1.jpg" width="468" height="456" alt="Figure: General Scheme of the project" /></p>

Revision as of 01:37, 21 October 2012

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Purple non-sulfur photosynthetic bacteria (PNSB) are metabolically versatile organisms that belong to the alpha-proteobacteria. This microorganisms are able to grow under a wide variety of environmental conditions, this is possible due to their sophisticated regulatory systems which coordinate metabolic changes. Our project aims to build two genetic control systems based on R. sphaeroides photosynthesis regulation (1).

The first one is the oxygen dependent system PrrBCA. It activates gene expression when the oxygen is low (2). The second system is the light/oxygen mediated system that strongly represses gene expression under aerobic conditions and allows transcription in the absence of oxygen and light (3).


To achieve this goal we designed a genetic circuit in which GFP expression is oxygen and light-dependent by the antirepression of PpsR and oxygen dependent by the activation of PrrA/B system. The lab work is accompanied by a computational model, which will provide a way of testing our knowledge of these systems.


Once, we characterize these regulatory circuits we aim to exploit R. palustris’ metabolic versatility, and use it as a microbial factory, that could work for the production economic valuable products using CO2 as carbon source.


We are planning to use the S04147 (University of Alberta iGEM Team 2007) to evaluate butanol production controlled by our systems. This would provide an interesting way to produce this biofuel using photosynthesis under anaerobic conditions.


Figure: General Scheme of the project

Figure: General Scheme of the project

  1. 1.Hunter CN, Daldal F, Thurnauer MC, Beatty JT: (2009) The Purple Phototrophic Bacteria. Springer; 200928. pp. 707–725.
  2. 2.Elsen S, Swem LR, Swem DL, Bauer CE. (2004). RegB/RegA, a highly conserved redoxresponding global two-component regulatory system. Microbiol. Mol. Biol. Rev. 68:263–79.
  3. 3.Shinji Masuda2 and Carl E. Bauer (2002) AppA Is a Blue Light Photoreceptor that Antirepresses Photosynthesis Gene Expression in Rhodobacter sphaeroides Cell, Vol. 110, 613–623, September 6, 2002.