Team/CINVESTAV-IPN-UNAM MX/Construction.htm

From 2012.igem.org

Revision as of 19:47, 23 October 2012 by Jhonatan (Talk | contribs)

Rho

Construction!

iGEM PROMOTER CHARACTERIZATION


We need to use a medium strength promoter for our constructions, so we characterize different promoters constitutive belonging to the isolated family from Anderson collection (J23101, J23102, J23104, J23107, J23108, J2311, and J23115) these promoters were assemble with GFP to determine promoter strength, using Victor X3 Multilabel Plate Reader.

figura1

figura2



Result

The characterization of the BioBricks shown in the following table:

Figura4

Figura5

 

 

 

 

 

The constructions that were made, were confirmed by double digestions. The following image shows a gel double digestion with XbaI and SpeI for confirmatory promoter constructs:












The constructions were cultivated in LB petri dishes with chloramphenicol and they were observated at UV light after it’s incubation.

Figura6Figura7


In the following graphs there is shown the GFP expression in function of th time and the realtive promoter force.

Figura9