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<p align="justify">With the valuable collaboration of the team iGEM-Alberta 2008, we link the the light-oxygen dependent regulation systems with the butanol production pathway and its electron-transfer-flavoprotein alpha and betha polypeptides, this constructions were sent by Dr. Douglas Ridgway. (Reference: <a href="https://2008.igem.org/Team:The_University_of_Alberta/Butanerd" target="_blank">Butanerd</a>)</p> | <p align="justify">With the valuable collaboration of the team iGEM-Alberta 2008, we link the the light-oxygen dependent regulation systems with the butanol production pathway and its electron-transfer-flavoprotein alpha and betha polypeptides, this constructions were sent by Dr. Douglas Ridgway. (Reference: <a href="https://2008.igem.org/Team:The_University_of_Alberta/Butanerd" target="_blank">Butanerd</a>)</p> | ||
Revision as of 01:21, 27 September 2012
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 With the valuable collaboration of the team iGEM-Alberta 2008, we link the the light-oxygen dependent regulation systems with the butanol production pathway and its electron-transfer-flavoprotein alpha and betha polypeptides, this constructions were sent by Dr. Douglas Ridgway. (Reference: Butanerd) Clostridium acetobutylicum is an anaerobic, spore-forming bacterium with the ability to ferment starch and sugars into solvents. In the past, it has been used for industrial production of acetone and butanol, until cheap crude oil rendered petrochemical synthesis more economically feasible. Both economic and environmental aspects have caused the pendulum to swing back again. Molecular biology has allowed a detailed understanding of genes and enzymes, required for solventogenesis.(1) We already constructed the following circuits, ready for testing in R. Palustris
We have to link these constructions into the vector pRK415, and conjugate R. sphearoides and R. palutris, to finally test our system and to produce butanol. References
1.- Fermentative butanol production: bulk chemical and biofuel. Ann N Y Acad Sci., Dürre P. 2008 Mar;1125:353-62.2.-Cong T. Trinh, Elucidating and reprogramming Escherichia coli metabolisms for obligate anaerobic n-butanol and isobutanol production Appl Microbiol Biotechnol DOI 10.1007/s00253-012-4197-73.-Masayuki Inui & Masako Suda & Sakurako Kimura & Kaori Yasuda & Hiroaki Suzuki & Hiroshi Toda & Shogo Yamamoto & Shohei Okino & Nobuaki Suzuki & Hideaki Yukawa Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli Appl Microbiol Biotechnol (2008) 77:1305–1316
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