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File:6-2,7-2 PUF-PIN Protein Purification.png 673×435 (280,925 bytes) - 23:00, 30 September 2012File:WT PUF-PIN, 6-2,7-2 PUF-PIN, WT PUF-aGFP Lysates.png 673×443 (190,403 bytes) - 23:01, 30 September 2012File:UCalgary2012 SurveyQuestion 2(graph 2) (new).PNG 343×244 (11,611 bytes) - 08:03, 3 October 2012File:UCalgary2012 Survey Question 2 (graph 2).PNG 473×285 (15,339 bytes) - 06:50, 3 October 2012File:BCA Analysis of WT, 6-2,7-2 PUF-PIN Proteins.png 910×651 (53,172 bytes) - 22:26, 3 October 2012File:Btn 2 over.jpg 100×17 (1,044 bytes) - 06:23, 16 July 2012File:IGEM RFP FINAL Page 2 sm1.JPG 954×1,233 (228,040 bytes) - 21:39, 1 May 2012File:2-110114223143.jpg 256×256 (24,812 bytes) - 08:58, 4 May 2012File:Image-2 PR.png 174×200 (45,826 bytes) - 15:09, 18 September 2012File:1,2,3,4,5,6,7,8 gel.jpg 520×255 (34,011 bytes) - 21:45, 7 June 2012File:Ucalgary2012 24.05.2012-Gel 2(jpeg).jpg 1,392×1,040 (21,243 bytes) - 20:57, 1 June 2012File:Helix 2 blue.jpg 640×480 (93,537 bytes) - 14:23, 6 June 2012File:2 Context.jpg 1,000×350 (168,610 bytes) - 04:22, 26 October 2012File:120705 (2).jpg 638×379 (85,898 bytes) - 09:13, 8 August 2012File:UCalgary2012Catechol assay (2).jpg 159×214 (2,800 bytes) - 01:14, 4 October 2012File:Line(2).jpg 677×666 (37,444 bytes) - 13:00, 17 July 2012File:HokkaidoU2012 120714 pT7-RBS on 1K3 pSB1C3 Ag43-dT on 1AK3 pSB1T3 Ligation1,2 coloP-No.jpg 528×576 (33,952 bytes) - 14:20, 14 July 2012File:V abnahme bei glykol(2).png 944×610 (16,539 bytes) - 00:34, 27 September 2012File:Exe2012Week 2 Diary Banner.jpg 980×228 (52,396 bytes) - 10:40, 17 August 2012File:HokkaidoU2012 120722 ag43 pcr-2% print.jpg 384×680 (23,857 bytes) - 05:59, 22 July 2012
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File:Gelpic010.jpg Lanes 1, 2 = pSB1C3-Plac-lacZ'-ccm, clones 1 and 2, digested with XbaI-PstI. Clone 2 looks as expected, clone 1 has an unexpected band around 0.6 kb.240×254 (46,611 bytes) - 18:39, 26 September 2012File:Gelpic005.jpg pSB1C3-cymA clones 1 and 2, testing internal restriction sites. Lanes 1, 2 = clones 1 and 2, NdeI.240×263 (46,143 bytes) - 18:29, 26 September 2012File:PCR Purified eGFP & plsr.JPG 6/21/12 EtBr Gel 2% Agarose Lane 1: NEB 2-log ladder (1 ug loaded)579×709 (82,839 bytes) - 17:38, 21 June 2012File:GECO on plate reader 96 wells.jpg ... were cultured on medium with 2% glucose (column 5 to 7) or on medium with 2% galactose (column 8 to 10). Since the GECO protein is under control of the733×514 (70,079 bytes) - 01:26, 27 September 2012File:Band with CAR success 50ng.JPG ...ained in the PET vector. Lane 1 contains the 1 kb plus ladder, while lanes 2,3 contained samples in which 5ng of original CAR+PET plasmid were used, lan1,017×661 (75,518 bytes) - 02:40, 22 August 2012File:Western-GFPgenerator.png Lane5:IPTG 0mM, sample 2µL<br> Lane6:IPTG 1mM, sample 2µL<br>389×527 (90,147 bytes) - 15:29, 19 September 2012File:Toluene degading pathway.jpg ...he genes from the Tod operon and what reaction each encode (TDO is Toluene 2,3-dioxygenase and is encoded by TodC1 and TodC2). TodA and TodB are electro ...11. Growth of Pseudomonas putida F1 on styrene requires increased catechol-2,3-dioxygenase activity, not a new hydrolase. Microbiology. 157, 89-98.1,800×727 (110,549 bytes) - 10:34, 24 September 2012File:Gelpic003.jpg lanes 3, 4 = pSB1C3-cymA clones 1 and 2, digested EcoRI. Band has size correct for linearised plasmid with the gene lanes 5, 6 = pSB1C3-cymA clones 1 and 2, double digested with EcoRI/SpeI.269×240 (45,374 bytes) - 18:23, 26 September 2012File:Gelpic006.jpg Lanes 1, 2 = EcoRI/SpeI digestion. Results appear as expected assuming 2 of the 3 ClaI sites are uncuttable due to overlapping dam methylation.240×274 (45,618 bytes) - 18:32, 26 September 2012- File:GFPAID-Zstack1-1.gif z-stack 6.09.12 (2)19 B (1 word) - 15:12, 18 September 2012
File:PUF2.png PUF Overview 2 uiuc700×500 (52,411 bytes) - 03:41, 29 May 2012File:CR02.jpg Figure 2. Coffee rust lesions often concentrate on the margins and tips of leaves. (463×815 (180,319 bytes) - 16:12, 29 June 2012File:Team-EPF-Lausanne Notebook 13 June 2012 gelbands.jpg ...he ladder. Seventh is the non-digested plasmid. In the rest we can see the 2 expected bands, though not exactly in the expected place.1,280×720 (163,344 bytes) - 18:29, 1 July 2012File:Peking TestBackground2.png test background 2960×540 (171,898 bytes) - 13:42, 16 July 2012File:TUM12 120716 PCR PALconsless.Tif PCR-result of PAL without consensussequence. band at 2,1kb.768×576 (886,374 bytes) - 22:08, 17 July 2012File:Team-EPF-Lausanne-gel-16.07.12.jpg 1,2: 1kb ladder.584×498 (82,954 bytes) - 23:24, 18 July 2012File:TUM12 120717 PCR PALconsens.Tif PCR-result of PAL with consensussequence. band at 2,1kb768×576 (886,398 bytes) - 22:12, 17 July 2012File:TUM12 120716 PCR PALconsless.jpg PCR-result of PAL without consensussequence. Expected band at 2,1kb.768×576 (51,073 bytes) - 22:18, 17 July 2012File:TUM12 120717 PAL+ prepGel.jpg Preparative Gel of PAL with consensussequence. Expected band at 2,1kb.699×476 (26,727 bytes) - 22:19, 17 July 2012File:TUM12 120717 PCR PALconsens.jpg PCR-result of PAL with consensussequence. Expected band at 2,1kb.768×576 (33,994 bytes) - 22:19, 17 July 2012
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