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Create the page "DNA Submission" on this wiki!

• Team:Cornell/testing/notebook/wetlab/3
  ...nd internal cut site (we expect lower mutation efficiency because template DNA is not methylated). Second, we transformed DH5a with the mutated plasmid. T ...cut site within the nah operon) worked. Unfortunately, there wasn't enough DNA to see anything when visualized on a gel. They decided to proceed with elec
  105 KB (17,618 words) - 03:59, 4 October 2012
• Team:Cornell/notebook/wetlab/august
  ...nd internal cut site (we expect lower mutation efficiency because template DNA is not methylated). Second, we transformed DH5a with the mutated plasmid. T ...cut site within the nah operon) worked. Unfortunately, there wasn't enough DNA to see anything when visualized on a gel. They decided to proceed with elec
  125 KB (19,514 words) - 22:59, 26 October 2012
• Team:KIT-Kyoto/Notebook-week4p
  <Tr><Td>　DNA sample　</Td><Td>　1uL　</Td></Tr> <Tr><Td>　DNA sample　</Td><Td>　1uL　</Td></Tr>
  15 KB (2,364 words) - 13:32, 26 September 2012
• Team:Calgary/Notebook/Hydrocarbon
  ...oolkit' were a restriction digest protocol, PCR purification, and finally, DNA construction digest. Aims moving forward include obtaining strains of the < ...ed by nanodrop to confirm successful plasmid extraction, and the resulting DNA concentrations were as follows: </p>
  101 KB (16,854 words) - 18:44, 21 September 2012
• Team:Calgary/Notebook/Denitrogenation
  ... these cultures, and plan on attempting to amplify genes from the isolated DNA next week. </p> ...3.5 ng/microlitre of <i>antB</i> DNA and 129 ng/microlitre of <i>carAc </i>DNA. These concentrations were both sufficient to begin a restriction digest an
  41 KB (7,115 words) - 00:16, 27 October 2012
• Regions/Europe/Pre-Jamboree
  <li><a href="#13">DNA submission</a></li> ...s://2012.igem.org/Regions/Europe/Transnatural">here</a> the details of the submission.
  9 KB (1,518 words) - 08:39, 22 September 2012
• Team:BostonU/Notebook
  ... with DVL0_ER (SJ4). We performed Gel Electrophoresis on this set of DNA.</li> ...protocol. We then quantified the amount of DNA we had using NanoDrop DNA quantification protocol andset up a restriction digest (SpeI, BSA, Bu
  46 KB (7,749 words) - 17:47, 26 October 2012
• Regions/Asia/Jamboree/Pre-regional Jamboree
  <li><a href="#13">DNA Submissions</a></li> ... this<a href="https://igem.org/Safety"> page </a> for more information.The submission due date is <strong>September 7th</strong>.
  24 KB (3,849 words) - 02:43, 4 October 2012
• Regions/Asia/pre-jamboree
  ... this<a href="https://igem.org/Safety"> page </a> for more information.The submission due date is <strong>September 7th</strong>. ... <a href="https://2012.igem.org/Safety">page</a> for more information. The submission due date will be on <strong>September 7th</strong>.
  18 KB (2,899 words) - 03:19, 22 August 2012
• Main Page Archive
  ...artsregistry.org/wiki/index.php/DNA_Submission_Instructions">BioBrick part DNA due to Registry</a></li> <li><i>submission <a href="https://2012.igem.org/Calendar_of_Events">deadlines</a> are coming
  70 KB (11,003 words) - 20:59, 14 November 2012
• Team:Calgary/Notebook/Desulfurization
  ...oolkit' were a restriction digest protocol, PCR purification, and finally, DNA construction digest. Aims moving forward include obtaining strains of the < ...tures, which were then miniprepped the following day to obtain the plasmid DNA of the putative <i>hpaC</i> biobrick. Digestions were performed on the mini
  65 KB (10,832 words) - 16:10, 3 September 2013
• Team:UNITN-Trento/Notebook
  ...roduct on the gel . I proceed with the restriction of CysE and pSB1C3 (the submission vector) with E and P, and then ligation and transformation in Nova Blue cel ...time we used the registry protocol; This time we used higher quantities of DNA. For the insert we used PCR products rather than a plasmid.<br/>
  82 KB (13,657 words) - 08:27, 24 September 2012
• Team:Cambridge/Overview/Labbook
  ...on of DNA = 360ng/&mu;l, so 2&mu;l needed for the desired 0.6&mu;g of DNA. DNA added to bacteria. *Nanodrop was used to determine the concentration of DNA in the biobrick after resuspension. The result reading was 103ng/ul.
  14 KB (2,109 words) - 02:36, 27 October 2012
• Team:TU Munich/Notebook/Meetings
  ...wn to work by a different research lab. It would be a good idea to use the DNA they submitted to the registry and make it functional (e.g. removing the fo *How do we get the physical DNA (ask a research lab to send it to us, extract from organisms via PCR, synth
  103 KB (15,460 words) - 13:23, 26 September 2012
• Team:St Andrews/Notebook
  ...Very quickly, "BioSilta", "GenScript", "Clontech", "Geneious", "Integrated DNA Technologies" and "Thermo Fisher" pledge their support and Team St Andrews ...ope" are all well received. Children and adults alike are fascinated when DNA is extracted from bananas, using everyday kitchen utensils, before their ey
  27 KB (4,526 words) - 02:47, 27 September 2012
• Requirements
  <li><strong>Part Submission:</strong> ...href="http://partsregistry.org/wiki/index.php/DNA_Submission_Instructions">submission instructions</a></li>
  5 KB (725 words) - 18:49, 4 September 2012
• Team:WashU/Week12
  We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as ...llowed by ligation of Z + TOPO, U + TOPO, and our main construct CS42S for submission.
  5 KB (772 words) - 02:26, 15 September 2012
• Team:UC Davis/Notebook/Protocols
  <li>DNA template</li> <li>T4 DNA Ligase
  49 KB (6,768 words) - 02:52, 27 October 2012
• World Championship Jamboree/Handbook
  ====DNA Submissions==== ...mission | DNA submission requirements]] for more information about the DNA submission process.
  35 KB (5,686 words) - 18:00, 23 October 2012
• Team:Rutgers/BEAS
  ..., LovTAP is a fusion protein. It consists of a light-response domain and a DNA binding domain, each of which are parts of other natural proteins.</p> <h4 class="shadow">DNA Binding</h4>
  12 KB (1,704 words) - 04:06, 4 October 2012
• Team:St Andrews/Omega-3-synthesis
  ...teria Synechocystis and the trypanosomatid Leishmania major. Combining the DNA code for elongase and desaturase enzymes, we are planning to convert a fatt ... a cyanobacterium. The trypanosomatid <i>Leishmania major</i> provided the DNA for the ELO 6 gene. Additionally, we used <i>Trypanosome cruzi</i> as a sec
  31 KB (3,639 words) - 23:15, 26 September 2012
• Team:WashU/Week11
  ...e one potentially successful PCR in the middle was obtained from the wrong DNA. <br> ...nd ran it on the same gel. Afterwards, we gel purified the three pieces of DNA. We finished off by ligating together the Z construct and C plasmid in one
  5 KB (866 words) - 02:21, 15 September 2012
• Team:Goettingen/Project/Bioinformatical Tool
  In this example, ApE is used to find the binding site on Lambda DNA for primer with a specific sequence. ...ev-com of string" (the primer may be identical to (a part of) the opposite DNA strand.) and click the "Find Next" button, as shown in Fig. 1.
  11 KB (1,782 words) - 19:12, 25 September 2012
• Team:Trieste/notebook2
  ...LL 37 into the pSB1C3 linearized backbone to make it ready for a potential submission.</br> ...loned the T5LacOperator inside the pSB1C3 plasmid, making it ready for the submission.
  4 KB (638 words) - 18:05, 26 October 2012
• Team:Michigan/Project
  ...r, one can build complex control circuits using combinations of invertible DNA sequences. We utilized the unidirectional recombinase HbiF to augment an ex ...ia coli</i> is regulated by the enzyme-catalyzed inversion of a segment of DNA, often labeled <i>fimS</i>, by a series of tyrosine recombinases of which F
  7 KB (1,152 words) - 03:10, 4 October 2012
• Team:British Columbia/Human Practices/IP FAQ
  ...ample, New England BioLabs has trademarked their own line of high-fidelity DNA polymerase under the name Phusion®. ...pproved patents can be held for a set number of years (e.g., 20 years from submission of application) before it becomes public domain. The time period for which
  24 KB (3,914 words) - 03:55, 4 October 2012
• Team:Cambridge/Lab book/Week 12
  '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of biobrick vector DNA]]''' ...d from Gibson products made on '''Check!!!''' were miniprepped and plasmid DNA extracted.
  6 KB (865 words) - 03:52, 27 September 2012
• Team:Wageningen UR/OutsideModification
  ...ordered to still make this part (figure 7) available for the registry, but submission before the wiki deadline will not be possible anymore. However, we think th ...e of whole plasmid PCR and Dpn1 digestion of methylated and hemimethylated DNA. An overview is given in figure 8.
  14 KB (1,975 words) - 20:48, 26 October 2012
• Team:Potsdam Bioware/Project/Collaboration
  ...nes specifically and team Freiburg standardizes the TAL proteins that bind DNA specifically, we thought it would be a great idea to combine our efforts. < ...-binding protein which can bind specifically to a 14 base pair long target DNA sequence. In case of our project, this sequence is located close to the CDR
  3 KB (440 words) - 17:10, 24 October 2012
• Team:Technion/Project/YES gates
  ... didn't get a working clone with any of the RNAPs in time for the BioBrick submission deadline. ...[http://partsregistry.org/Part:BBa_J06504 BBa_J06504] contained some other DNA. As a result, we used PCR to get mCherry from [http://partsregistry.org/Par
  6 KB (1,011 words) - 00:50, 27 September 2012
• Team:University College London/Notebook/Week13
  ...on from our marine bacteria ''Oceanibulbus Indolifex'' using Qiagen QIAamp DNA Mini Kit. In the afternoon, we attempted to PCR genes antifreeze and mercur <div class="notebook-regional"> '''Regional Jamboree: Abstract Submission''' - Bethan finished our abstract for the Regional Jamboree while Aurelija
  5 KB (682 words) - 22:22, 26 September 2012
• Team:Rutgers/BIB2
  ...s the pyruvate is broken down into Acetyl-CoA.  The aceF coding region of DNA codes for a protein that acts as a dihydrolipoyl transacetylase, which is a <td><h2 class="shadow">Parts Submission</h2></td>
  9 KB (1,127 words) - 03:12, 4 October 2012
• Regions/Europe/PreJamboree
  <h3><a name="13"></a>DNA submission</h3>
  1 KB (224 words) - 21:05, 13 February 2012
• Regions/Asia/Jamboree
  ...umentation , including documentation for all medal criteria, BioBrick Part DNA to the Registry</a>,
  12 KB (1,310 words) - 03:24, 18 August 2013
• Team:British Columbia/Team
  ...d mutagenesis of the DszC gene to remove the two illegal PstI sites before submission to the Registry.</div> ...l Hieter's yeast genetics lab at UBC. My own research project looks at RNA:DNA hybrids and their effect on genome stability. I have been advising iGEM tea
  13 KB (1,939 words) - 02:35, 4 October 2012
• Team:CU-Boulder/Project
  ...led either due to its large size (6478bp) or due to an inaccurate registry submission. Several other teams have tried to use their part since 2010 but none have ...ng biofilm in order for the spore to escape and travel to a new location. DNA in biofilms is responsible for increased viscoelastic and adhesive properti
  16 KB (2,564 words) - 03:26, 4 October 2012
• Team:St Andrews/metal-binding
  ...line program <a href="http://molbiol.ru/eng/scripts/01_19.html">Protein to DNA</a>.</p> <p>The gBlocks alone were ligated into the iGEM pSB1C3 vector for submission.</p>
  23 KB (3,168 words) - 01:09, 27 September 2012
• Team:NYU Gallatin/Project/Cloning
  </ul><p>This worked very well, and resulted in the submission of three new parts to the BioBrick Library (Bba_K850000, Bba_K850001, Bba_K ..._K850001 plasmid using Xba1 and Pst1 and gel-purified. These two pieces of DNA were ligated together to produce a plasmid containing the AGM1 and Xba1 par
  18 KB (1,934 words) - 03:15, 4 October 2012
• Team:Technion/19 September 2012
  ...d on. These starters will be used for minipreping the liquid DNA for parts submission.<br>
  2 KB (308 words) - 11:58, 26 September 2012
• Team:Edinburgh/Project/Bioelectric-Interface/Bio-electric-Interface-BioBricks-Cloning
  - Gel purification and DNA purification: for <i>ccm</i> and several ligation attempts for other genes ...gene contains an internal PstI site which needs to be mutated out prior to submission and use.
  14 KB (2,255 words) - 19:52, 26 October 2012
• Team:Cambridge/Project/Results
  Having obtained our DNA, the first test was a rather crude β-Galactosidase assay using cells cultu ...racterisation, as it the construct tended to be lost, and necessitated its submission to the registry in a low copy number backbone (with permission from HQ).
  36 KB (4,449 words) - 03:56, 27 September 2012
• Team:Queens Canada/Notebook/Week17
  ...lass="tableizer-firstrow"><th>Date</th><th>Protocol</th><th>People</th><th>DNA (if relevant)</th><th>Quantities and Parameters (if relevant)</th><th>Notes ...f the variable D3 domain), but were unsuccessful in ligating them into the submission plasmid. Other things we worked on included xylE characterization using cat
  15 KB (2,544 words) - 04:04, 27 October 2012
• Team:Cambridge/Ratiometrica/Results
  The biobrick DNA has been sequenced and sent to the registry. ...racterisation, as it the construct tended to be lost, and necessitated its submission to the registry in a low copy number backbone (with permission from HQ).
  9 KB (1,395 words) - 01:10, 27 October 2012