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Create the page "10% DMSO stocks" on this wiki!

Team:TU Munich/Notebook/Labjournal
<a href="#Week_10">Week 10</a> 13.8-19.8 <a href="#Week_14">Week 14</a> 10.9-16.9 

793 KB (114,680 words) - 14:38, 3 May 2013
Team:Potsdam Bioware/Lab/Labjournal/September
Transformation via manual, 10 µl of miniprep sample and 1 µL of pCEP4 were used * vortex for 10 s 3 x

236 KB (34,819 words) - 22:05, 26 October 2012
Team:Bielefeld-Germany/Labjournal
<li><a href="#10">Week 9</a></li> <li><a href="#11">Week 10</a></li>

333 KB (50,931 words) - 03:59, 27 October 2012
Team:MIT/Notebook
...24 G, 224 PI-SceI, started gel at 100V at 9:40, run for 25 mins, finish at 10:05</td></tr> ...ments">Inoculated culture 1 from -80C iGem box using 1000:1 Amp (10 ul for 10 ml), split into 2 x 5ml tubes. Put in 37 C shaker at 9:00 PM</td></tr>

76 KB (12,442 words) - 22:58, 26 October 2012
Team:British Columbia/ConsortiaNotebook
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53 KB (9,047 words) - 06:59, 28 September 2012
Team:UIUC-Illinois/Notebook/LabNotebook
<li><a name="week10puf" >Week 10</a></li> <td>- Made glycerol stocks of C0012, E0030, I732017, PSB1A3, PSB1AK3, PSB1K3 and put them in the -80 f

59 KB (9,788 words) - 05:14, 26 October 2012
Team:British Columbia/BiobrickConstruction
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30 KB (5,082 words) - 03:34, 4 October 2012
Team:TU Darmstadt/Labjournal/Degradation
* 2-5 1.PCR, 6-9 2.PCR, 10-13 3.PCR ** c(Promo-LacO-RBS-Phoa-FsC)=10 ng/µL

139 KB (19,958 words) - 02:51, 27 September 2012
Team:NTNU Trondheim/Notebook
...ned in the notebook, see [https://2012.igem.org/Team:NTNU_Trondheim/Stocks Stocks] and [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs]. For ...protocols]. The concentration after gel extraction was: cplld = 10,6 ng/µl.

95 KB (15,220 words) - 17:15, 25 September 2012
Team:NTNU Trondheim/Test/Notebook
...ned in the notebook, see [https://2012.igem.org/Team:NTNU_Trondheim/Stocks Stocks] and [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs]. For ...protocols]. The concentration after gel extraction was: cplld = 10,6 ng/µl.

95 KB (15,213 words) - 09:43, 21 September 2012
Team:Grenoble/Biology/Notebook/August
<li>Lane 2: pSB4C5 (miniprep) PCR product (DMSO)</li> <li>Lane 3: pSB4C5 (miniprep) PCR product (DMSO)</li>

14 KB (2,258 words) - 10:07, 26 September 2012
Team:NTNU Trondheim/Notebook/July
...e culture with nitrogen gas to remove oxygen. Samples were collected every 10 minutes. Updated information about stored DNA samples and glycerol stocks on the wiki.

51 KB (7,919 words) - 17:23, 25 September 2012
Team:Grenoble/Biology/Notebook/July/week 30
...FP, and on colony to amplify RBS_Cya. We did the PCR both with and without DMSO. <li>Lane 2: pSB4K5 PCR product (DMSO)</li>

15 KB (2,516 words) - 14:04, 26 September 2012
Team:Stanford-Brown/Protocols
-We have 50x stocks of BG11 in the fridge that are or can be aliquoted into 20mL samples '''Antibiotic Stocks'''

34 KB (5,685 words) - 22:10, 3 October 2012
Team:Wisconsin-Madison/protocol
<li>10 mM EDTA (pH 8.0)</li> Prepare Solution I from standard stocks in batches of -100 ml, autoclave for 15 minutes at 15 psi (l.05 kg/cm2 ) on

42 KB (7,189 words) - 18:05, 3 October 2012
Team:MIT/MaterialsAndMethods
...omponents to a 1.5 mL tube at room temperature and mix: attB-PCR product (=10 ng/uL; final amount ~15-150 ng) 1-7 uL , Donor vector (150 ng/uL) 1 uL ,TE ...o terminate the reaction. Vortex briefly. Incubate samples at 37°C for 10 minutes. </LI>

42 KB (6,746 words) - 03:12, 4 October 2012
Team:TU Darmstadt/Protocols/Metabolism
* Inoculate 10 ml of LB-Media with an E. coli colony and incubate at 37 °C overnight * Centrifuge the cells for 10 min at 5000 rpm and 4 °C

17 KB (2,545 words) - 23:00, 26 September 2012
Team:Calgary/Notebook/Hydrocarbon
...2011 (see https://2011.igem.org/Team:Washington/alkanebiosynthesis). These stocks are as follows: <li> 10 mL of 1 mg/mL Thiamine </li>

101 KB (16,854 words) - 18:44, 21 September 2012
Team:UNITN-Trento/Protocols
filter:blur(add=false, direction=270 strength=10); -ms-filter:blur(add=false, direction=270 strength=10);

45 KB (6,225 words) - 11:07, 25 September 2012
Team:Columbia-Cooper-NYC/Columbia notebook 2
* Relocated and reorganized the iGEM biobrick kit and glycerol stocks * Added 10µl of ho1 and PcyA to 5ml of antibiotics

37 KB (5,174 words) - 07:36, 8 January 2013
Team:BostonU/Notebook
...1.1 and 13.1 to achive a higher concentration of DNA. We ran gel of MS8.1, 10.1, 13.1,11.1 to amplify more DNA template to achieve sufficient DNA concent <li> Ran MgCl2 gradient of MF3 PCR to troubleshoot, along with DMSO separately. 

46 KB (7,749 words) - 17:47, 26 October 2012
Team:ETH Zurich/MaterialMethods
...1:5 plasmid backbone : Insert . The Ligation mix is incubated for at least 10 min at room temperature or for at least 1 hour at 16°C before the ligase i ====Glycerol Stocks====

7 KB (988 words) - 02:49, 27 October 2012
Team:Potsdam Bioware/Lab/Labjournal/June
* ca. 80 (100 µL) Stocks frozen competent E. coli XL1 Blue *Preparation of glycerol stocks (one of XL1 with AID (BBa_K103001), one of XL1 with pcdna5/frt)

18 KB (2,579 words) - 19:10, 25 September 2012
Team:Westminster/Protocols
z-index:10; ...up to a maximum of 1x 10^6 cells for 30 sec. in microfuge. If more than 1X 10^6 cells are used, the reagent volumes will have to be increased accordingly

25 KB (3,655 words) - 19:09, 12 November 2012
Team:Calgary/Notebook/Killswitch
On Thursday we verified our transformation process with a cPCR where we ran 10 colonies from each plate with our controls. We ran a gel of the PCR product Lanes 6-9: PhoQ (~671 bp) Lanes 10-13: mntR (~500 bp)

34 KB (5,463 words) - 03:23, 27 October 2012
Team:Bonn/Protocols
* centrifugation at 4 °C, 4500 g, 10 min * add 1,5 ml DMSO

7 KB (1,000 words) - 22:24, 26 September 2012
Team:University College London/Protocols/Week12/3
All primers were prepared to a concentration of 1pmol.uL, from 100pmol.uL stocks. Primers ordered from MWG operon. | 5X Phusion HF buffer || 4 µl || 10 µl || 1X

1 KB (126 words) - 22:02, 26 September 2012
Team:University College London/Week12YanikaExp4
...n and were prepared to a working concentration of 1pmol/uL from 100pmol/uL stocks. |5X Phusion HF Buffer || 4 µl || 10 µl || 1X

1 KB (203 words) - 21:50, 26 September 2012