http://2012.igem.org/wiki/index.php?title=Special:Contributions/Sallen&feed=atom&limit=50&target=Sallen&year=&month=2012.igem.org - User contributions [en]2024-03-29T09:13:50ZFrom 2012.igem.orgMediaWiki 1.16.0http://2012.igem.org/Team:Gaston_Day_SchoolTeam:Gaston Day School2012-10-03T21:13:29Z<p>Sallen: </p>
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<h1><center>Detection of Heavy Metal Contaminants in Water</h1><br><br />
</html><br />
<br />
Despite improvements in water quality, contaminants still interfere with farming in many of the world’s biomes. For 2012 the Gaston Day School iGEM team’s project is to help solve this problem by using the existing registry of parts to create new heavy metal detectors. We have shifted our project from last year’s nitrate detector in order to focus on cadmium, arsenic, and lead contaminants in water. These metals are known to be used in insecticides, fungicides, and fertilizers and are also byproducts of industrial processes such as smelting. If ingested they can cause numerous health problems (more information can be found at the bottom of the page). To detect each metal, we constructed sensors by using multiple promoters to narrow the range of the heavy metals down to one or two contaminants. Then we combined it with GFP reporters to create the new part. GFP was used because our spectrophotometer can accurately measure it.<br />
<br/><br />
<br/><br />
Once the parts were created, they were tested for accuracy and sensitivity. Many farmers need a way to measure the amount of heavy metals in water to determine whether the levels are dangerous; therefore, each heavy metal detector must be as sensitive as their respective federal limits in water. As the project continues, we plan to test the safety of the engineered bacteria and create survivorship curves as they are released into different environments, similar to last year’s testing. When the final kit is constructed we plan to include the heavy metal detectors and all components necessary to run, accurately measure, and safely dispose of the tests. This kit will help agricultural and environmental fields make improvements in safety.<br />
<br />
<br/><br />
<br/><br />
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<tr><th align="center">iGEM 2012 Gaston Day School</th></tr><br />
<br />
<tr><td align="center"><br />
<img src="https://static.igem.org/mediawiki/2011/9/91/GordonlogofGDS.jpg" HEIGHT="300" WIDTH="340" BORDER="0" name="show"><br />
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<option value="https://static.igem.org/mediawiki/2012/9/97/Awgdslogo.png">Gaston Day School 2012 iGEM Logo</option><br />
<option value="https://static.igem.org/mediawiki/2012/e/ec/Teampic1.JPG">Team Picture</option><br />
<option value="https://static.igem.org/mediawiki/2011/7/70/Steven_useGDS.jpg">Steven "Dedicated Team Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/d/d9/ParthuploadGDS.jpg">Parth "The Dedicated Team Co-Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/46/GordonuploadGDS.jpg">Gordon "Normal?"</option><br />
<option value="https://static.igem.org/mediawiki/2011/e/e3/Sam_USEGDS.jpg">Sam "Sam the Man"</option><br />
<option value="https://static.igem.org/mediawiki/2012/6/66/Willpic.JPG">Will "The Peasant"</option><br />
<option value="https://static.igem.org/mediawiki/2012/d/d7/Audreypic.JPG">Audrey</option><br />
<option value="https://static.igem.org/mediawiki/2011/c/cc/SignproblemsGDS.jpg">iGEM Warning Sign</option><br />
<option value="https://static.igem.org/mediawiki/2012/2/2d/Pcrupload.jpg">Our PCR Machine!!</option><br />
<option value="https://static.igem.org/mediawiki/2011/6/6c/AutoclavefinalGDS.jpg">Autoclave</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/4d/LabareaGDS.jpg">Chemical Storage Room</option><br />
<option value="https://static.igem.org/mediawiki/2011/2/25/LandfarmGDS.jpg">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/f/fc/AgricultureGDS.jpg ">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/1/11/HwboardGDS.jpg">iGEM Board at Gaston Day School!<br />
<option value="https://static.igem.org/mediawiki/2011/e/eb/StevenparthGDS.jpg">Steven & Parth Working on the Website</option><br />
<option value="https://static.igem.org/mediawiki/2011/a/a5/GelboxGDS.jpg">Gel Electrophoresis Box</option><br />
<option value="https://static.igem.org/mediawiki/2011/3/3d/Qwirkle_uploadGDS.jpg">...BREAKTIME!!!<br />
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'''Links to More Info:'''<br />
<br />
<div style="text-align: left;"><br />
*http://www.lef.org/protocols/health_concerns/heavy_metal_toxicity_01.htm<br />
*http://www.psr.org/environment-and-health/confronting-toxics/heavy-metals/<br />
*http://www.water.ncsu.edu/watershedss/info/hmetals.html<br />
*http://www.atsdr.cdc.gov/<br />
*http://www.atsdr.cdc.gov/spl/</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_SchoolTeam:Gaston Day School2012-10-03T21:12:17Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Detection of Heavy Metal Contaminants in Water</h1><br><br />
</html><br />
<br />
Despite improvements in water quality, contaminants still interfere with farming in many of the world’s biomes. For 2012 the Gaston Day School iGEM team’s project is to help solve this problem by using the existing registry of parts to create new heavy metal detectors. We have shifted our project from last year’s nitrate detector in order to focus on cadmium, arsenic, and lead contaminants in water. These metals are known to be used in insecticides, fungicides, and fertilizers and are also byproducts of industrial processes such as smelting. If ingested they can cause numerous health problems (more information can be found at the bottom of the page). To detect each metal, we constructed sensors by using multiple promoters to narrow the range of the heavy metals down to one or two contaminants. Then we combined it with GFP reporters to create the new part. GFP was used because our spectrophotometer can accurately measure it.<br />
<br/><br />
<br/><br />
Once the parts were created, they were tested for accuracy and sensitivity. Many farmers need a way to measure the amount of heavy metals in water to determine whether the levels are dangerous; therefore, each heavy metal detector must be as sensitive as their respective federal limits in water. As the project continues, we plan to test the safety of the engineered bacteria and create survivorship curves as they are released into different environments, similar to last year’s testing. When the final kit is constructed we plan to include the heavy metal detectors and all components necessary to run, accurately measure, and safely dispose of the tests. This kit will help agricultural and environmental fields make improvements in safety.<br />
<br />
<br/><br />
<br/><br />
<br/><br />
<br/><br />
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<tr><th align="center">iGEM 2012 Gaston Day School</th></tr><br />
<br />
<tr><td align="center"><br />
<img src="https://static.igem.org/mediawiki/2011/9/91/GordonlogofGDS.jpg" HEIGHT="300" WIDTH="340" BORDER="0" name="show"><br />
</td></tr><br />
<br />
<tr><td align="center" style="border:1px black solid;"><br />
<select name="slide" onChange="rotate(this.selectedIndex);"><br />
<option value="https://static.igem.org/mediawiki/2012/9/97/Awgdslogo.png">Gaston Day School 2012 iGEM Logo</option><br />
<option value="https://static.igem.org/mediawiki/2012/e/ec/Teampic1.JPG">Team Picture</option><br />
<option value="https://static.igem.org/mediawiki/2011/7/70/Steven_useGDS.jpg">Steven "Dedicated Team Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/d/d9/ParthuploadGDS.jpg">Parth "The Dedicated Team Co-Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/46/GordonuploadGDS.jpg">Gordon "Normal?"</option><br />
<option value="https://static.igem.org/mediawiki/2011/e/e3/Sam_USEGDS.jpg">Sam "Sam the Man"</option><br />
<option value="https://static.igem.org/mediawiki/2012/6/66/Willpic.JPG">Will "The Peasant"</option><br />
<option value="https://static.igem.org/mediawiki/2012/d/d7/Audreypic.JPG">Audrey</option><br />
<option value="https://static.igem.org/mediawiki/2011/c/cc/SignproblemsGDS.jpg">iGEM Warning Sign</option><br />
<option value="https://static.igem.org/mediawiki/2012/c/cc/SignproblemsGDS.jpg">Our PCR Machine!!</option><br />
<option value="https://static.igem.org/mediawiki/2011/6/6c/AutoclavefinalGDS.jpg">Autoclave</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/4d/LabareaGDS.jpg">Chemical Storage Room</option><br />
<option value="https://static.igem.org/mediawiki/2011/2/25/LandfarmGDS.jpg">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/f/fc/AgricultureGDS.jpg ">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/1/11/HwboardGDS.jpg">iGEM Board at Gaston Day School!<br />
<option value="https://static.igem.org/mediawiki/2011/e/eb/StevenparthGDS.jpg">Steven & Parth Working on the Website</option><br />
<option value="https://static.igem.org/mediawiki/2011/a/a5/GelboxGDS.jpg">Gel Electrophoresis Box</option><br />
<option value="https://static.igem.org/mediawiki/2011/3/3d/Qwirkle_uploadGDS.jpg">...BREAKTIME!!!<br />
<br />
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src="http://c.gigcount.com/wildfire/IMP/CXNID=2000002.0NXC/bT*xJmx*PTEzMTcxNjg5MTA1MTUmcHQ9MTMxNzE2ODkxOTcwOSZwPTIwNzM1MSZkPSZnPTEmbz*2MmM1MjgxOTA2NTk*NjgxYTU2/NjRhNWM5N2JhMjdiNCZvZj*w.gif" /><a href="http://www.visitormap.org/" target="_top"><img src="http://www.visitormap.org/map/m:qddznhzvzxphwenm/s:1/c:ff0000/p:cross/y:6.png" alt="Free Visitor Maps at VisitorMap.org" border="0"></a><br><a href="http://www.visitormap.org/">Get a FREE visitor map for your site!</a><br />
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<br />
'''Links to More Info:'''<br />
<br />
<div style="text-align: left;"><br />
*http://www.lef.org/protocols/health_concerns/heavy_metal_toxicity_01.htm<br />
*http://www.psr.org/environment-and-health/confronting-toxics/heavy-metals/<br />
*http://www.water.ncsu.edu/watershedss/info/hmetals.html<br />
*http://www.atsdr.cdc.gov/<br />
*http://www.atsdr.cdc.gov/spl/</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_SchoolTeam:Gaston Day School2012-10-03T21:11:23Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Detection of Heavy Metal Contaminants in Water</h1><br><br />
</html><br />
<br />
Despite improvements in water quality, contaminants still interfere with farming in many of the world’s biomes. For 2012 the Gaston Day School iGEM team’s project is to help solve this problem by using the existing registry of parts to create new heavy metal detectors. We have shifted our project from last year’s nitrate detector in order to focus on cadmium, arsenic, and lead contaminants in water. These metals are known to be used in insecticides, fungicides, and fertilizers and are also byproducts of industrial processes such as smelting. If ingested they can cause numerous health problems (more information can be found at the bottom of the page). To detect each metal, we constructed sensors by using multiple promoters to narrow the range of the heavy metals down to one or two contaminants. Then we combined it with GFP reporters to create the new part. GFP was used because our spectrophotometer can accurately measure it.<br />
<br/><br />
<br/><br />
Once the parts were created, they were tested for accuracy and sensitivity. Many farmers need a way to measure the amount of heavy metals in water to determine whether the levels are dangerous; therefore, each heavy metal detector must be as sensitive as their respective federal limits in water. As the project continues, we plan to test the safety of the engineered bacteria and create survivorship curves as they are released into different environments, similar to last year’s testing. When the final kit is constructed we plan to include the heavy metal detectors and all components necessary to run, accurately measure, and safely dispose of the tests. This kit will help agricultural and environmental fields make improvements in safety.<br />
<br />
<br/><br />
<br/><br />
<br/><br />
<br/><br />
<html><br />
<script type="text/javascript"><br />
// Author: ricocheting.com<br />
// Description: slideshow that allows visitors to flip through a series of images on your website<br />
<br />
var x=0;<br />
<br />
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<form name="ff"><br />
<table cellpadding="3" style="border:1px black solid;border-collapse:collapse;" align="center"><br />
<tr><th align="center">iGEM 2012 Gaston Day School</th></tr><br />
<br />
<tr><td align="center"><br />
<img src="https://static.igem.org/mediawiki/2011/9/91/GordonlogofGDS.jpg" HEIGHT="300" WIDTH="340" BORDER="0" name="show"><br />
</td></tr><br />
<br />
<tr><td align="center" style="border:1px black solid;"><br />
<select name="slide" onChange="rotate(this.selectedIndex);"><br />
<option value="https://static.igem.org/mediawiki/2012/9/97/Awgdslogo.png">Gaston Day School 2012 iGEM Logo</option><br />
<option value="https://static.igem.org/mediawiki/2012/e/ec/Teampic1.JPG">Team Picture</option><br />
<option value="https://static.igem.org/mediawiki/2011/7/70/Steven_useGDS.jpg">Steven "Dedicated Team Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/d/d9/ParthuploadGDS.jpg">Parth "The Dedicated Team Co-Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/46/GordonuploadGDS.jpg">Gordon "Normal?"</option><br />
<option value="https://static.igem.org/mediawiki/2011/e/e3/Sam_USEGDS.jpg">Sam "Sam the Man"</option><br />
<option value="https://static.igem.org/mediawiki/2012/6/66/Willpic.JPG">Will "The Peasant"</option><br />
<option value="https://static.igem.org/mediawiki/2012/d/d7/Audreypic.JPG">Audrey</option><br />
<option value="https://static.igem.org/mediawiki/2011/c/cc/SignproblemsGDS.jpg">iGEM Warning Sign</option><br />
<option value="<option value="https://static.igem.org/mediawiki/2011/c/cc/SignproblemsGDS.jpg">Our PCR Machine!!</option><br />
<option value="https://static.igem.org/mediawiki/2011/6/6c/AutoclavefinalGDS.jpg">Autoclave</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/4d/LabareaGDS.jpg">Chemical Storage Room</option><br />
<option value="https://static.igem.org/mediawiki/2011/2/25/LandfarmGDS.jpg">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/f/fc/AgricultureGDS.jpg ">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/1/11/HwboardGDS.jpg">iGEM Board at Gaston Day School!<br />
<option value="https://static.igem.org/mediawiki/2011/e/eb/StevenparthGDS.jpg">Steven & Parth Working on the Website</option><br />
<option value="https://static.igem.org/mediawiki/2011/a/a5/GelboxGDS.jpg">Gel Electrophoresis Box</option><br />
<option value="https://static.igem.org/mediawiki/2011/3/3d/Qwirkle_uploadGDS.jpg">...BREAKTIME!!!<br />
<br />
</select><br />
</td></tr><br />
<tr><td align="center" style="border:1px black solid;"><br />
<br />
<input type="button" name="fa" onClick="this.value=((this.value=='Stop')?'Start':'Stop');auto();" value="Start" title="Autoplay" style="width:75px;" /><br />
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<!--- The Mission, Experiments ---><br />
<br />
<html><br />
<center><br />
<img style="visibility:hidden;width:0px;height:0px;" border=0 width=0 height=0<br />
src="http://c.gigcount.com/wildfire/IMP/CXNID=2000002.0NXC/bT*xJmx*PTEzMTcxNjg5MTA1MTUmcHQ9MTMxNzE2ODkxOTcwOSZwPTIwNzM1MSZkPSZnPTEmbz*2MmM1MjgxOTA2NTk*NjgxYTU2/NjRhNWM5N2JhMjdiNCZvZj*w.gif" /><a href="http://www.visitormap.org/" target="_top"><img src="http://www.visitormap.org/map/m:qddznhzvzxphwenm/s:1/c:ff0000/p:cross/y:6.png" alt="Free Visitor Maps at VisitorMap.org" border="0"></a><br><a href="http://www.visitormap.org/">Get a FREE visitor map for your site!</a><br />
</center><br />
</html><br />
<br/><br />
<br />
'''Links to More Info:'''<br />
<br />
<div style="text-align: left;"><br />
*http://www.lef.org/protocols/health_concerns/heavy_metal_toxicity_01.htm<br />
*http://www.psr.org/environment-and-health/confronting-toxics/heavy-metals/<br />
*http://www.water.ncsu.edu/watershedss/info/hmetals.html<br />
*http://www.atsdr.cdc.gov/<br />
*http://www.atsdr.cdc.gov/spl/</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_SchoolTeam:Gaston Day School2012-10-03T21:10:16Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Detection of Heavy Metal Contaminants in Water</h1><br><br />
</html><br />
<br />
Despite improvements in water quality, contaminants still interfere with farming in many of the world’s biomes. For 2012 the Gaston Day School iGEM team’s project is to help solve this problem by using the existing registry of parts to create new heavy metal detectors. We have shifted our project from last year’s nitrate detector in order to focus on cadmium, arsenic, and lead contaminants in water. These metals are known to be used in insecticides, fungicides, and fertilizers and are also byproducts of industrial processes such as smelting. If ingested they can cause numerous health problems (more information can be found at the bottom of the page). To detect each metal, we constructed sensors by using multiple promoters to narrow the range of the heavy metals down to one or two contaminants. Then we combined it with GFP reporters to create the new part. GFP was used because our spectrophotometer can accurately measure it.<br />
<br/><br />
<br/><br />
Once the parts were created, they were tested for accuracy and sensitivity. Many farmers need a way to measure the amount of heavy metals in water to determine whether the levels are dangerous; therefore, each heavy metal detector must be as sensitive as their respective federal limits in water. As the project continues, we plan to test the safety of the engineered bacteria and create survivorship curves as they are released into different environments, similar to last year’s testing. When the final kit is constructed we plan to include the heavy metal detectors and all components necessary to run, accurately measure, and safely dispose of the tests. This kit will help agricultural and environmental fields make improvements in safety.<br />
<br />
<br/><br />
<br/><br />
<br/><br />
<br/><br />
<html><br />
<script type="text/javascript"><br />
// Author: ricocheting.com<br />
// Description: slideshow that allows visitors to flip through a series of images on your website<br />
<br />
var x=0;<br />
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<table cellpadding="3" style="border:1px black solid;border-collapse:collapse;" align="center"><br />
<tr><th align="center">iGEM 2012 Gaston Day School</th></tr><br />
<br />
<tr><td align="center"><br />
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<option value="https://static.igem.org/mediawiki/2012/9/97/Awgdslogo.png">Gaston Day School 2012 iGEM Logo</option><br />
<option value="https://static.igem.org/mediawiki/2012/e/ec/Teampic1.JPG">Team Picture</option><br />
<option value="https://static.igem.org/mediawiki/2011/7/70/Steven_useGDS.jpg">Steven "Dedicated Team Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/d/d9/ParthuploadGDS.jpg">Parth "The Dedicated Team Co-Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/46/GordonuploadGDS.jpg">Gordon "Normal?"</option><br />
<option value="https://static.igem.org/mediawiki/2011/e/e3/Sam_USEGDS.jpg">Sam "Sam the Man"</option><br />
<option value="https://static.igem.org/mediawiki/2012/6/66/Willpic.JPG">Will "The Peasant"</option><br />
<option value="https://static.igem.org/mediawiki/2012/d/d7/Audreypic.JPG">Audrey</option><br />
<option value="https://static.igem.org/mediawiki/2011/c/cc/SignproblemsGDS.jpg">iGEM Warning Sign</option><br />
<option value="<option value="https://static.igem.org/mediawiki/2011/c/cc/SignproblemsGDS.jpg">iGEM Warning Sign</option>">Our PCR Machine!!</option><br />
<option value="https://static.igem.org/mediawiki/2011/6/6c/AutoclavefinalGDS.jpg">Autoclave</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/4d/LabareaGDS.jpg">Chemical Storage Room</option><br />
<option value="https://static.igem.org/mediawiki/2011/2/25/LandfarmGDS.jpg">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/f/fc/AgricultureGDS.jpg ">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/1/11/HwboardGDS.jpg">iGEM Board at Gaston Day School!<br />
<option value="https://static.igem.org/mediawiki/2011/e/eb/StevenparthGDS.jpg">Steven & Parth Working on the Website</option><br />
<option value="https://static.igem.org/mediawiki/2011/a/a5/GelboxGDS.jpg">Gel Electrophoresis Box</option><br />
<option value="https://static.igem.org/mediawiki/2011/3/3d/Qwirkle_uploadGDS.jpg">...BREAKTIME!!!<br />
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<img style="visibility:hidden;width:0px;height:0px;" border=0 width=0 height=0<br />
src="http://c.gigcount.com/wildfire/IMP/CXNID=2000002.0NXC/bT*xJmx*PTEzMTcxNjg5MTA1MTUmcHQ9MTMxNzE2ODkxOTcwOSZwPTIwNzM1MSZkPSZnPTEmbz*2MmM1MjgxOTA2NTk*NjgxYTU2/NjRhNWM5N2JhMjdiNCZvZj*w.gif" /><a href="http://www.visitormap.org/" target="_top"><img src="http://www.visitormap.org/map/m:qddznhzvzxphwenm/s:1/c:ff0000/p:cross/y:6.png" alt="Free Visitor Maps at VisitorMap.org" border="0"></a><br><a href="http://www.visitormap.org/">Get a FREE visitor map for your site!</a><br />
</center><br />
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<br/><br />
<br />
'''Links to More Info:'''<br />
<br />
<div style="text-align: left;"><br />
*http://www.lef.org/protocols/health_concerns/heavy_metal_toxicity_01.htm<br />
*http://www.psr.org/environment-and-health/confronting-toxics/heavy-metals/<br />
*http://www.water.ncsu.edu/watershedss/info/hmetals.html<br />
*http://www.atsdr.cdc.gov/<br />
*http://www.atsdr.cdc.gov/spl/</div>Sallenhttp://2012.igem.org/File:Pcrupload.jpgFile:Pcrupload.jpg2012-10-03T21:09:28Z<p>Sallen: </p>
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<div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/SafetyTeam:Gaston Day School/Safety2012-10-03T20:56:45Z<p>Sallen: </p>
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<h1><center>Safety</h1><br />
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''''' 1. Would any project ideas raise safety issues in terms of ______? '''''<br />
::'''''A. Researcher Safety: ''''' <br />
::: As with any science experiment, there are risks involved with our project. Our team works with bleach which can cause irritation and a burning sensation if improperly exposed through inhalation, ingestion, or prolonged exposure to skin. However, any person is able to buy bleach from their local grocery store. Even so, we take care to prevent any issues. Also, our team works with ''E. coli'' K12. It is a version of ''E. coli'' that is widely studied and found to be very safe and practical for research. In fact, it is one of the most-studied bacteria of all time. ''E. coli'' K12 has no known survival mechanisms in the environment and has a very low risk of causing harm to organisms. ''E. coli'' K12 is in attenuated strain that is approved for use in high school classrooms. Even with antibiotic resistance, the amount of harm caused to most organisms would be minimal. The only real danger would be if a bacterium was able to conjugate with another type of bacteria that was more harmful and transfer its resistances. The chances of this happening are small due to the fact that the bacterium is held in a lab that most high school students do not enter (separate room that remains locked) and because proper safety precautions are followed relative to our biosafety level when inside this lab, including proper safety equipment and diligent hand washing.<br />
::'''''B. Public Safety: '''''<br />
:::There are not many reasonable threats to the safety and health of the public if the project were to be released. This is mainly because we are in the biosafety level (BSL) 1 category which limits what materials we can use. In saying this, bleach can be purchased by almost anyone at their local supermarket or grocery store. Therefore, the risk is no different than someone buying the bleach and using it as a household chemical. Also, ''E. coli'' K12 can be purchased by the public. Therefore, it is again low risk. As long as proper regulations are followed specific to their safety level, there is minimal risk. For the general public, the only way the bacterium may cause harm is through the spread of antibiotic resistance to chloramphenicol. The reason for this particular drug resistance is because this is what our team uses as a selective marker to attain the final product. It only becomes risky when this resistance is spread to more harmful bacteria.<br />
::'''''C. Environmental Safety? '''''<br />
:::There are no unreasonable, adverse effects associated with bleach being introduced into the environment. ''E. coli'' K12 has no known way of surviving in the environment and cannot produce spores. It is also not able to colonize the gut of an organism which makes it a very low environmental threat. From our machine, there are no real environmental threats. Again, this arises from working within BSL 1 and from the public having access to the same materials we use. Therefore, they are all relatively stable and safe. There is no real toxicity other than bleach, but it can be purchased at many stores and locations around the world and is only harmful from intentional misuse (ex. ingesting very large quantities, inhaling large amounts). Our machine itself is not pathogenic but if it is able to conjugate with other possibly pathogenic bacteria, then it may cause some degree of harm. If the bacterium is isolated then this will not happen and may be used safely.<br />
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''''' 2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? '''''<br />
:::The BioBrick parts that we built do not raise any major safety concerns. A few minor risks are associated such as the potential conjugation and sharing of antibiotic resistance. However, the largest safety issue for this year’s project is in the testing of our sensors. We have sensors that test for heavy metal contaminants. Hence, we need to use heavy metals to test the functionality and sensitivity of our detectors. We used Lead Acetate (0.1 M) and Cadmium Nitrate (1 M), both of which are available for purchase from a science supply store. <br />
:''''' If yes, '''''<br />
::'''''A. Did you document these issues in the Registry?'''''<br />
::: We have not added parts to the registry at this point; proper documentation will be included when our BioBrick parts are added to the registry.<br />
::''''' B. How did you manage to handle the safety issue?'''''<br />
::: Extra care was taken when working with these materials such as the use of a fume hood and proper lab technique. Since we are in a high school setting, we must abide by all BSL 1 guidelines and regulations. This severely limits our available options. Many regulations are imposed upon our team; a few include: a sink must be present in the lab, controlled access (locked doors), no eating or drinking in lab area, decontamination of surfaces after lab work, and a lab supervisor. All work is supervised by our instructor, Ms. Anne Byford; she has a background in molecular genetics and trains all team members to ensure safety. She has also supervised and gone through the specific methods and processes we use to isolate, cut, ligate, and view the DNA used to create our bacterium. This also includes proper lab etiquette and how to use equipment such as a microcentrifuge, pipettes, and gel electrophoresis boxes. Proper safety technique is used when handling all materials. We treat everything as if it were hazardous. <br />
::''''' C. How could other teams learn from your experience?'''''<br />
::: Other teams could potentially learn from our experience through proper research on their biosafety level and its respective regulations. Also, teams could look at the materials used to test their machines, not just the construction process itself.<br />
''''' 3. Is there a local biosafety group, committee, or review board at your institution?'''''<br />
::'''''A. If “yes,” what does your local biosafety group think about your project?'''''<br />
<br />
:::At Gaston Day School we do not have a set of our own biosafety rules. In saying this, we do follow all of the regulations and rules from BSL 1 and operate under the supervision of our advisor, Ms. Anne Byford, who has much knowledge in the biological field.<br />
:::All of our iGEM projects at Gaston Day School are approved by the Head of the School, Dr. Richard Rankin, the Head of Middle and Upper School, Mr. Greg Rainey, and lastly the Board of Directors to ensure the safety of our team and school.<br />
<br />
::'''''B. If “no,” which specific biosafety rules or guidelines do you have to consider in your country?'''''<br />
:::The United States of America’s biosafety regulations are controlled by the CDC (Center for Disease Control and Prevention). More information can be found in the link below.<br />
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''''' 4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices, and systems be made even safer through biosafety engineering?''''' <br />
::'''''A. '''''Safety is the most important part of the competition. Safety must be stressed upon and needs to be a priority in all labs. To help with this, each team and advisor should have to fill out a survey that measures the safety in their lab and also makes sure that they are staying within the regulations of their BSL. Also, photos should be submitted of the lab to show that they have the proper safety regulation and requirements.<br />
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''Citations:'' <br />
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1. http://epa.gov/biotech_rule/pubs/fra/fra004.htm<br />
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2. http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf pages 30-33.<br />
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3. http://www.cdc.gov/biosafety/</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/ProjectTeam:Gaston Day School/Project2012-10-03T20:55:20Z<p>Sallen: </p>
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Heavy metal contaminants in water pose serious health problems; the lungs, liver, kidneys, blood, digestive system, and <br />
the nervous system are all affected by contamination. The Agency for Toxic Substances and Disease registry released a Priority List of Hazardous Substances (ASTDR). Heavy metals accounted for almost half of the top 10 substances; therefore, we have constructed a set of sensors that detects heavy metal contaminants in water. Our sensors provide an inexpensive, simple, and visual test for Arsenic (the number one substance from ASTDR’s list), Lead (number two), and Cadmium (number seven). One sensor paired a promoter responsive to both Cadmium and Arsenic with GFP as a reporter. Another was created for the detection of Lead and a third sensor was specific to cadmium. Use of the detector could potentially save lives around the world through early detection of the contamination.<br />
<br />
The kit will include all necessary components for running the test and then decontaminating the resulting growth to prevent release of the engineered bacteria into the environment. We will include a very simple mechanism for killing or denaturing the bacteria in our detector kit – bleach. Bleach is highly effective at killing bacteria and is readily available to the average person.<br />
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We built the GFP Cadmium and Arsenic Sensors using NEB's BioBrick Construction Kit. We digested the promoter responsive to Cadmium (BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The same process was used for Arsenic (BBa_J33201). The promoters were Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium. We chose Cadmium because of our BSL. We are not allowed to have Arsenic on campus for testing since it is a known poison. Schematics of the 2 processes are shown below.<br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Constructsarscad.jpg|900px]]<br />
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The same process was used to create our Lead Sensor. A lead responsive promoter (BBa_I721001) was paired with GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://openwetware.org/wiki/IGEM:Brown/2007 2007 Brown iGEM team]. The schematic process is shown below. <br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:leadconst.jpg|500px]]<br />
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<h1><center>The Experiments</h1><br />
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We tested out detectors (protocol can be found [https://static.igem.org/mediawiki/2012/d/da/2012GDS8-20.23.24.pdf here]) and measured the sensitivity using a spectrophotometer. We measured in Flu./OD which is the amount of fluorescence per OD (at 600nm). At first, we encountered many problems. We noticed that all of our dilutions, including the control were approximately the same. We realized that LB (Luria Broth, our growth medium) fluoresces naturally; hence, it masked the effects of the GFP. To solve this problem, we tried to run our tests using M9 Salts as a minimal growth medium. However, our bacteria were unable to grow in the medium. After this, our solution was to grow in LB, spin down the bacteria, then resuspend in PBS (phosphate buffer solution). Lastly, our spectrophotometer still continued to show the same fluorescence reading for most samples except the control. We then realized that the readings had maxed. Serial dilutions were used to determine accurate measurements. <br />
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<h1><center>Results</h1><br />
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Overall, our tests show that all 3 of our detectors worked properly, even the lead detector with the "non-functional" lead promoter.<br />
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The graphs shown below represent our results (in Fluorescence per OD600) for the three detectors.<br />
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[[File:Arsgraph.jpg|center|400px|caption]]<br />
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[[File:Cadgraphuse.jpg|center|400px|caption]]<br />
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[[File:Leadgraph.jpg|center|400px|caption]]</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/TeamTeam:Gaston Day School/Team2012-10-03T20:54:26Z<p>Sallen: </p>
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<h1><center>Who We Are</h1><br />
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Our team consists primarily of high school juniors and is based out of Gaston Day School, a Pre-K through 12 school in Gastonia, North Carolina. With Ms. Anne Byford's guidance, we are taking part in our fourth iGEM competition.<br />
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<div style="text-align: left;"><br />
[[File:Teampic1.JPG|right|400px|caption]]<br />
'''Advisor:''' Anne Byford<br />
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'''Juniors:'''<br />
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*'''Dedicated Leader''': Steven Allen <br />
*'''Dedicated Co-Leader''': Parth Patel<br />
*'''A normal student...or am I?''': Gordon Ellison<br />
*'''Sam the Man''': Samuel DuBois<br />
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'''Sophomores:'''<br />
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*'''The Peasant''': Will Reiber<br />
*'''Young Grasshopper''': Audrey Weiss<br />
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File:Tmsteven.jpg| <center>'''Steven Allen'''<br />
File:Tmparth.png|<center>'''Parth Patel'''<br />
File:Tmgordon.jpg|<center>'''Gordon Ellison'''<br />
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</gallery><br />
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<gallery widths=250px heights=250px perrow=3><br />
File:Tmsam.jpg|<center>'''Samuel DuBois'''<br />
File:Willpicuseonline.jpg|<center>'''Will Reiber'''<br />
File:Awgdsaudrey.JPG|<center>'''Audrey Weiss'''<br />
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<h1><center>What We Did</h1><br />
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We developed Cadmium, Arsenic, and Lead detectors by combining responsive promoters with Green Fluorescent Protein reporters.<br />
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<h1><center>Where We're From</h1><br />
</html><br />
<div style="text-align: left;"><br />
Founded in 1967, Gaston Day School is a non-sectarian, college preparatory institution for grades<br />
Pre-K through 12. Throughout our history, nearly 100% of Gaston Day School's classes have entered<br />
four-year colleges and universities immediately upon graduation. Located in<br />
Gastonia, North Carolina, the school serves communities in a five-county area: Gaston, Lincoln,<br />
Cleveland, Mecklenburg and York counties. Enrollment is approximately 500 students.<br />
Gaston Day School is accredited by the Southern Association of Colleges and Schools. The<br />
school is a member of the Southern Association of Independent Schools, the National<br />
Association of Independent Schools, and the North Carolina Association of Independent Schools.<br />
Each of the students on the team has completed (or is currently in) Honors Biology, Honors<br />
Chemistry, AP Biology, AP Chemistry, Honors Physics or some combination thereof. <br />
<br />
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<br />
<div class="center" style="width:auto; margin-center:auto; margin-right:auto;">'''Gaston Day School'''</div><br />
<html><br />
<iframe width="425" height="350" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="https://maps.google.com/maps?f=q&amp;source=s_q&amp;hl=en&amp;geocode=&amp;q=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;aq=1&amp;oq=Gaston+Day+School&amp;sll=35.170517,-79.860994&amp;sspn=7.621033,14.27124&amp;ie=UTF8&amp;hq=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;ll=35.201736,-81.141128&amp;spn=0.018235,0.032015&amp;t=p&amp;output=embed"></iframe><br /><small><a href="https://maps.google.com/maps?f=q&amp;source=embed&amp;hl=en&amp;geocode=&amp;q=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;aq=1&amp;oq=Gaston+Day+School&amp;sll=35.170517,-79.860994&amp;sspn=7.621033,14.27124&amp;ie=UTF8&amp;hq=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;ll=35.201736,-81.141128&amp;spn=0.018235,0.032015&amp;t=p" style="color:#0000FF;text-align:center">View Larger Map</a></small><br />
</html></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/TeamTeam:Gaston Day School/Team2012-10-03T20:54:05Z<p>Sallen: </p>
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<h1><center>Who We Are</h1><br />
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Our team consists primarily of high school juniors and is based out of Gaston Day School, a Pre-K through 12 school in Gastonia, North Carolina. With Ms. Anne Byford's guidance, we are taking part in our fourth iGEM competition.<br />
<br />
<div style="text-align: left;"><br />
[[File:Teampic1.JPG|right|400px|caption]]<br />
'''Advisor:''' Anne Byford<br />
<br />
'''Juniors:'''<br />
<br />
*'''Dedicated Leader''': Steven Allen <br />
*'''Dedicated Co-Leader''': Parth Patel<br />
*'''A normal student...or am I?''': Gordon Ellison<br />
*'''Sam the Man''': Samuel DuBois<br />
<br />
'''Sophomores:'''<br />
<br />
*'''The Peasant''': Will Reiber<br />
*'''Young Grasshopper''': Audrey Weiss<br />
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File:Tmsteven.jpg| <center>'''Steven Allen'''<br />
File:Tmparth.png|<center>'''Parth Patel'''<br />
File:Tmgordon.jpg|<center>'''Gordon Ellison'''<br />
<br />
</gallery><br />
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<gallery widths=250px heights=250px perrow=3><br />
File:Tmsam.jpg|<center>'''Samuel DuBois'''<br />
File:Willpicuseonline.jpg|<center>'''Will Reiber'''<br />
File:Awgdsaudrey.JPG|<center>'''Audrey Weiss'''<br />
<br />
</gallery><br />
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<html><br />
<br><br />
<h1><center>What We Did</h1><br />
</html><br />
We developed Cadmium, Arsenic, and Lead detectors by combining responsive promoters with Green Fluorescent Protein reporters.<br />
<br />
<br />
<html><br />
<br><br />
<h1><center>Where We're From</h1><br />
</html><br />
Founded in 1967, Gaston Day School is a non-sectarian, college preparatory institution for grades<br />
Pre-K through 12. Throughout our history, nearly 100% of Gaston Day School's classes have entered<br />
four-year colleges and universities immediately upon graduation. Located in<br />
Gastonia, North Carolina, the school serves communities in a five-county area: Gaston, Lincoln,<br />
Cleveland, Mecklenburg and York counties. Enrollment is approximately 500 students.<br />
Gaston Day School is accredited by the Southern Association of Colleges and Schools. The<br />
school is a member of the Southern Association of Independent Schools, the National<br />
Association of Independent Schools, and the North Carolina Association of Independent Schools.<br />
Each of the students on the team has completed (or is currently in) Honors Biology, Honors<br />
Chemistry, AP Biology, AP Chemistry, Honors Physics or some combination thereof. <br />
<br />
<br />
<br />
<div class="center" style="width:auto; margin-center:auto; margin-right:auto;">'''Gaston Day School'''</div><br />
<html><br />
<iframe width="425" height="350" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="https://maps.google.com/maps?f=q&amp;source=s_q&amp;hl=en&amp;geocode=&amp;q=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;aq=1&amp;oq=Gaston+Day+School&amp;sll=35.170517,-79.860994&amp;sspn=7.621033,14.27124&amp;ie=UTF8&amp;hq=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;ll=35.201736,-81.141128&amp;spn=0.018235,0.032015&amp;t=p&amp;output=embed"></iframe><br /><small><a href="https://maps.google.com/maps?f=q&amp;source=embed&amp;hl=en&amp;geocode=&amp;q=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;aq=1&amp;oq=Gaston+Day+School&amp;sll=35.170517,-79.860994&amp;sspn=7.621033,14.27124&amp;ie=UTF8&amp;hq=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;ll=35.201736,-81.141128&amp;spn=0.018235,0.032015&amp;t=p" style="color:#0000FF;text-align:center">View Larger Map</a></small><br />
</html></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:52:41Z<p>Sallen: </p>
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<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
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For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
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Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:52:25Z<p>Sallen: </p>
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<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
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For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
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Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:50:26Z<p>Sallen: </p>
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<br><br />
<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
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For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
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Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:50:11Z<p>Sallen: </p>
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<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
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For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
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</html><br />
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<center><br />
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Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:49:20Z<p>Sallen: </p>
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<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
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For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
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<align=left><br />
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Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:48:58Z<p>Sallen: </p>
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<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
</p><br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
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<html><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
<br />
<p align="right"><br />
<br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:48:15Z<p>Sallen: </p>
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<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
</p><br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
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<html><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
<p align="right"><br />
<br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:47:20Z<p>Sallen: </p>
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<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
</p><br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
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<html><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
<br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:46:48Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
<p align="right"><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
</p><br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
<br/><br />
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</html><br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
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For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
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<br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:44:23Z<p>Sallen: </p>
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<h1><center>Outreach</h1><br />
<justify><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
<br/><br />
<br/><br />
<html><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
<br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:44:03Z<p>Sallen: </p>
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<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
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<br><br />
<br><br />
<h1><center>Outreach</h1><br />
</html><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our project" This "BioBrick" produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
</html><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
<br/><br />
<br/><br />
<html><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
<br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_SchoolTeam:Gaston Day School2012-10-03T20:40:44Z<p>Sallen: </p>
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<h1><center>Detection of Heavy Metal Contaminants in Water</h1><br><br />
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Despite improvements in water quality, contaminants still interfere with farming in many of the world’s biomes. For 2012 the Gaston Day School iGEM team’s project is to help solve this problem by using the existing registry of parts to create new heavy metal detectors. We have shifted our project from last year’s nitrate detector in order to focus on cadmium, arsenic, and lead contaminants in water. These metals are known to be used in insecticides, fungicides, and fertilizers and are also byproducts of industrial processes such as smelting. If ingested they can cause numerous health problems (more information can be found at the bottom of the page). To detect each metal, we constructed sensors by using multiple promoters to narrow the range of the heavy metals down to one or two contaminants. Then we combined it with GFP reporters to create the new part. GFP was used because our spectrophotometer can accurately measure it.<br />
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<br/><br />
Once the parts were created, they were tested for accuracy and sensitivity. Many farmers need a way to measure the amount of heavy metals in water to determine whether the levels are dangerous; therefore, each heavy metal detector must be as sensitive as their respective federal limits in water. As the project continues, we plan to test the safety of the engineered bacteria and create survivorship curves as they are released into different environments, similar to last year’s testing. When the final kit is constructed we plan to include the heavy metal detectors and all components necessary to run, accurately measure, and safely dispose of the tests. This kit will help agricultural and environmental fields make improvements in safety.<br />
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<tr><th align="center">iGEM 2012 Gaston Day School</th></tr><br />
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<tr><td align="center"><br />
<img src="https://static.igem.org/mediawiki/2011/9/91/GordonlogofGDS.jpg" HEIGHT="300" WIDTH="340" BORDER="0" name="show"><br />
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<option value="https://static.igem.org/mediawiki/2012/9/97/Awgdslogo.png">Gaston Day School 2012 iGEM Logo</option><br />
<option value="https://static.igem.org/mediawiki/2012/e/ec/Teampic1.JPG">Team Picture</option><br />
<option value="https://static.igem.org/mediawiki/2011/7/70/Steven_useGDS.jpg">Steven "Dedicated Team Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/d/d9/ParthuploadGDS.jpg">Parth "The Dedicated Team Co-Leader"</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/46/GordonuploadGDS.jpg">Gordon "Normal?"</option><br />
<option value="https://static.igem.org/mediawiki/2011/e/e3/Sam_USEGDS.jpg">Sam "Sam the Man"</option><br />
<option value="https://static.igem.org/mediawiki/2012/6/66/Willpic.JPG">Will "The Peasant"</option><br />
<option value="https://static.igem.org/mediawiki/2012/d/d7/Audreypic.JPG">Audrey</option><br />
<option value="https://static.igem.org/mediawiki/2011/c/cc/SignproblemsGDS.jpg">iGEM Warning Sign</option><br />
<option value="https://static.igem.org/mediawiki/2011/6/6c/AutoclavefinalGDS.jpg">Autoclave</option><br />
<option value="https://static.igem.org/mediawiki/2011/4/4d/LabareaGDS.jpg">Chemical Storage Room</option><br />
<option value="https://static.igem.org/mediawiki/2011/2/25/LandfarmGDS.jpg">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/f/fc/AgricultureGDS.jpg ">Farm Land...Potential Place of Use for the Heavy Metal Detector</option><br />
<option value="https://static.igem.org/mediawiki/2011/1/11/HwboardGDS.jpg">iGEM Board at Gaston Day School!<br />
<option value="https://static.igem.org/mediawiki/2011/e/eb/StevenparthGDS.jpg">Steven & Parth Working on the Website</option><br />
<option value="https://static.igem.org/mediawiki/2011/a/a5/GelboxGDS.jpg">Gel Electrophoresis Box</option><br />
<option value="https://static.igem.org/mediawiki/2011/3/3d/Qwirkle_uploadGDS.jpg">...BREAKTIME!!!<br />
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<!--- The Mission, Experiments ---><br />
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<center><br />
<img style="visibility:hidden;width:0px;height:0px;" border=0 width=0 height=0<br />
src="http://c.gigcount.com/wildfire/IMP/CXNID=2000002.0NXC/bT*xJmx*PTEzMTcxNjg5MTA1MTUmcHQ9MTMxNzE2ODkxOTcwOSZwPTIwNzM1MSZkPSZnPTEmbz*2MmM1MjgxOTA2NTk*NjgxYTU2/NjRhNWM5N2JhMjdiNCZvZj*w.gif" /><a href="http://www.visitormap.org/" target="_top"><img src="http://www.visitormap.org/map/m:qddznhzvzxphwenm/s:1/c:ff0000/p:cross/y:6.png" alt="Free Visitor Maps at VisitorMap.org" border="0"></a><br><a href="http://www.visitormap.org/">Get a FREE visitor map for your site!</a><br />
</center><br />
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<br />
'''Links to More Info:'''<br />
<br />
*http://www.lef.org/protocols/health_concerns/heavy_metal_toxicity_01.htm<br />
*http://www.psr.org/environment-and-health/confronting-toxics/heavy-metals/<br />
*http://www.water.ncsu.edu/watershedss/info/hmetals.html<br />
*http://www.atsdr.cdc.gov/<br />
*http://www.atsdr.cdc.gov/spl/</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:21:35Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
</html><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
<br/><br />
<br/><br />
<html><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
<br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a.Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:20:58Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
</html><br />
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
<br/><br />
<br/><br />
<html><br />
<center><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
Here are the directions we gave each participant <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a.Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-03T20:20:39Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<br><br />
<h1><center>Outreach</h1><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br><br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
</html><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|900px]]<br />
</div><br />
<br/><br />
<br/><br />
<html><br />
<center><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
</html><br />
Here are the directions we gave each participant: <br/><br />
:1.Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2.Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3.Add 1 ml of detergent using a dropper <br/><br />
:4.Swirl to mix (be gentle) <br/><br />
:5.Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a.Make a layer of alcohol on top of the water <br/><br />
:6.DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7.Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8.We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|400px]]<br /> DNA Necklace souvenir for one dollar.<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/AttributionsTeam:Gaston Day School/Attributions2012-10-03T20:08:25Z<p>Sallen: </p>
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<h1><center>Attributions & Contributions</h1><br />
</html><br />
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Gaston Day School hosts our iGEM team and provides a facility over the summer. Additional supporters are listed below.<br />
<br />
Ms. Anne Byford has contributed and provided guidance for our Gaston Day School iGEM team. Ms. Byford has a Master's Degree in Medical Genetics from Oregon Health Sciences University.<br />
<br />
The coding for the slideshow under the "Home" page is thanks to the HTML-generator developed by ricocheting.com: [http://www.ricocheting.com/code/javascript/html-generator/image-slideshow HTML-Slideshow Generator]<br />
<br />
Initial coding for the "drop-down" menu on our pages came from the [https://2011.igem.org/Team:Washington 2011 Washington iGEM Team]. <br />
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<br />
'''''Our 2012 Sponsors:'''''<br />
<br />
Ivana Chan & Family<br />
<br />
Sandra & Bill Hall<br />
<br />
[http://usa.autodesk.com/ Autodesk]<br />
<br />
[http://www.neb.com/nebecomm/default.asp New England Biolabs]<br />
<br />
[http://gastonday.org/ Gaston Day School]<br />
<br />
[http://markergene.com/ Marker Gene Technologies]<br />
<br />
<br />
<br />
<center><br />
<gallery widths=147px heights=130px perrow=5><br />
File:autodesklogo.jpg|[http://usa.autodesk.com/ <center>Autodesk]<br />
File:nebGDS.jpg|[http://www.neb.com/nebecomm/default.asp <center>New England Biolabs]<br />
File:Gaston_Day_School_logo.png|[http://gastonday.org/ <center>Gaston Day School]<br />
File:Mgtlogoigem.jpg|[http://markergene.com/ <center>Marker Gene Technologies]<br />
</gallery></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/AttributionsTeam:Gaston Day School/Attributions2012-10-03T20:07:59Z<p>Sallen: </p>
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<h1><center>Attributions & Contributions</h1><br />
</html><br />
<br />
<br />
Gaston Day School hosts our iGEM team and provides a facility over the summer. Additional supporters are listed below.<br />
<br />
<br />
Ms. Anne Byford has contributed and provided guidance for our Gaston Day School iGEM team. Ms. Byford has a Master's Degree in Medical Genetics from Oregon Health Sciences University.<br />
<br />
The coding for the slideshow under the "Home" page is thanks to the HTML-generator developed by ricocheting.com: [http://www.ricocheting.com/code/javascript/html-generator/image-slideshow HTML-Slideshow Generator]<br />
<br />
Initial coding for the "drop-down" menu on our pages came from the [https://2011.igem.org/Team:Washington 2011 Washington iGEM Team]. <br />
<br />
<br />
<br />
'''''Our 2012 Sponsors:'''''<br />
<br />
Ivana Chan & Family<br />
<br />
Sandra & Bill Hall<br />
<br />
[http://usa.autodesk.com/ Autodesk]<br />
<br />
[http://www.neb.com/nebecomm/default.asp New England Biolabs]<br />
<br />
[http://gastonday.org/ Gaston Day School]<br />
<br />
[http://markergene.com/ Marker Gene Technologies]<br />
<br />
<br />
<br />
<center><br />
<gallery widths=147px heights=130px perrow=5><br />
File:autodesklogo.jpg|[http://usa.autodesk.com/ <center>Autodesk]<br />
File:nebGDS.jpg|[http://www.neb.com/nebecomm/default.asp <center>New England Biolabs]<br />
File:Gaston_Day_School_logo.png|[http://gastonday.org/ <center>Gaston Day School]<br />
File:Mgtlogoigem.jpg|[http://markergene.com/ <center>Marker Gene Technologies]<br />
</gallery></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/ProjectTeam:Gaston Day School/Project2012-10-03T14:26:35Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Overall Project</h1><br />
</html><br />
{|align="justify"<br />
|<br />
Heavy metal contaminants in water pose serious health problems; the lungs, liver, kidneys, blood, digestive system, and <br />
the nervous system are all affected by contamination. The Agency for Toxic Substances and Disease registry released a Priority List of Hazardous Substances (ASTDR). Heavy metals accounted for almost half of the top 10 substances; therefore, we have constructed a set of sensors that detects heavy metal contaminants in water. Our sensors provide an inexpensive, simple, and visual test for Arsenic (the number one substance from ASTDR’s list), Lead (number two), and Cadmium (number seven). One sensor paired a promoter responsive to both Cadmium and Arsenic with GFP as a reporter. Another was created for the detection of Lead and a third sensor was specific to cadmium. Use of the detector could potentially save lives around the world through early detection of the contamination.<br />
<br />
The kit will include all necessary components for running the test and then decontaminating the resulting growth to prevent release of the engineered bacteria into the environment. We will include a very simple mechanism for killing or denaturing the bacteria in our detector kit – bleach. Bleach is highly effective at killing bacteria and is readily available to the average person.<br />
|-<br />
|<br />
|}<br />
<html><br />
<br><br />
<h1><center>Project Details</h1><br />
</html><br />
We built the GFP Cadmium and Arsenic Sensors using NEB's BioBrick Construction Kit. We digested the promoter responsive to Cadmium (BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The same process was used for Arsenic (BBa_J33201). The promoters were Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium. We chose Cadmium because of our BSL. We are not allowed to have Arsenic on campus for testing since it is a known poison. Schematics of the 2 processes are shown below.<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Constructsarscad.jpg|900px]]<br />
</div><br />
<br />
<br />
<br />
The same process was used to create our Lead Sensor. A lead responsive promoter (BBa_I721001) was paired with GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://openwetware.org/wiki/IGEM:Brown/2007 2007 Brown iGEM team]. The schematic process is shown below. <br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:leadconst.jpg|500px]]<br />
</div><br />
<br />
<html><br />
<br><br />
<h1><center>The Experiments</h1><br />
</html><br />
We tested out detectors (protocol can be found [https://static.igem.org/mediawiki/2012/d/da/2012GDS8-20.23.24.pdf here]) and measured the sensitivity using a spectrophotometer. We measured in Flu./OD which is the amount of fluorescence per OD (at 600nm). At first, we encountered many problems. We noticed that all of our dilutions, including the control were approximately the same. We realized that LB (Luria Broth, our growth medium) fluoresces naturally; hence, it masked the effects of the GFP. To solve this problem, we tried to run our tests using M9 Salts as a minimal growth medium. However, our bacteria were unable to grow in the medium. After this, our solution was to grow in LB, spin down the bacteria, then resuspend in PBS (phosphate buffer solution). Lastly, our spectrophotometer still continued to show the same fluorescence reading for most samples except the control. We then realized that the readings had maxed. Serial dilutions were used to determine accurate measurements. <br />
<br />
<html><br />
<br><br />
<h1><center>Results</h1><br />
</html><br />
<br />
Overall, our tests show that all 3 of our detectors worked properly, even the lead detector with the "non-functional" lead promoter.<br />
<br />
The graphs shown below represent our results (in Fluorescence per OD600) for the three detectors.<br />
<br/><br />
<br/><br />
<br/><br />
[[File:Arsgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Cadgraphuse.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Leadgraph.jpg|center|400px|caption]]</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/ProjectTeam:Gaston Day School/Project2012-10-02T11:54:56Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Overall Project</h1><br />
</html><br />
{|align="justify"<br />
|<br />
Heavy metal contaminants in water pose serious health problems; the lungs, liver, kidneys, blood, digestive system, and <br />
the nervous system are all affected by contamination. The Agency for Toxic Substances and Disease registry released a Priority List of Hazardous Substances (ASTDR). Heavy metals accounted for almost half of the top 10 substances; therefore, we have constructed a set of sensors that detects heavy metal contaminants in water. Our sensors provide an inexpensive, simple, and visual test for Arsenic (the number one substance from ASTDR’s list), Lead (number two), and Cadmium (number seven). One sensor paired a promoter responsive to both Cadmium and Arsenic with GFP as a reporter. Another was created for the detection of Lead and a third sensor was specific to cadmium. Use of the detector could potentially save lives around the world through early detection of the contamination.<br />
<br />
The kit will include all necessary components for running the test and then decontaminating the resulting growth to prevent release of the engineered bacteria into the environment. We will include a very simple mechanism for killing or denaturing the bacteria in our detector kit – bleach. Bleach is highly effective at killing bacteria and is readily available to the average person.<br />
|-<br />
|<br />
|}<br />
<html><br />
<br><br />
<h1><center>Project Details</h1><br />
</html><br />
We built the GFP Cadmium and Arsenic Sensors using NEB's BioBrick Construction Kit. We digested the promoter responsive to both Cadmium and Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoters were Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively. Schematics of the 2 processes are shown below.<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Constructsarscad.jpg|900px]]<br />
</div><br />
<br />
<br />
<br />
The same process was used to create our Lead Sensor. A lead responsive promoter (BBa_I721001) was paired with GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://openwetware.org/wiki/IGEM:Brown/2007 2007 Brown iGEM team]. The schematic process is shown below. <br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:leadconst.jpg|500px]]<br />
</div><br />
<br />
<html><br />
<br><br />
<h1><center>The Experiments</h1><br />
</html><br />
We tested out detectors (protocol can be found [https://static.igem.org/mediawiki/2012/d/da/2012GDS8-20.23.24.pdf here]) and measured the sensitivity using a spectrophotometer. We measured in Flu./OD which is the amount of fluorescence per OD (at 600nm). At first, we encountered many problems. We noticed that all of our dilutions, including the control were approximately the same. We realized that LB (Luria Broth, our growth medium) fluoresces naturally; hence, it masked the effects of the GFP. To solve this problem, we tried to run our tests using M9 Salts as a minimal growth medium. However, our bacteria were unable to grow in the medium. After this, our solution was to grow in LB, spin down the bacteria, then resuspend in PBS (phosphate buffer solution). Lastly, our spectrophotometer still continued to show the same fluorescence reading for most samples except the control. We then realized that the readings had maxed. Serial dilutions were used to determine accurate measurements. <br />
<br />
<html><br />
<br><br />
<h1><center>Results</h1><br />
</html><br />
<br />
Overall, our tests show that all 3 of our detectors worked properly, even the lead detector with the "non-functional" lead promoter.<br />
<br />
The graphs shown below represent our results (in Fluorescence per OD600) for the three detectors.<br />
<br/><br />
<br/><br />
<br/><br />
[[File:Arsgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Cadgraphuse.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Leadgraph.jpg|center|400px|caption]]</div>Sallenhttp://2012.igem.org/File:Cadgraphuse.jpgFile:Cadgraphuse.jpg2012-10-02T11:54:41Z<p>Sallen: </p>
<hr />
<div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/AttributionsTeam:Gaston Day School/Attributions2012-10-02T02:31:44Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<br />
<br />
<br />
<br />
<html><br />
<br><br />
<h1><center>Attributions & Contributions</h1><br />
</html><br />
<br />
<br />
Gaston Day School hosts our iGEM team and provides a facility over the summer. Additional supporters are listed below.<br />
<br />
<br />
Ms. Anne Byford has contributed and provided guidance for our Gaston Day School iGEM team. Ms. Byford has a Master's Degree in Medical Genetics from Oregon Health Sciences University.<br />
<br />
The coding for the slideshow under the "Home" page is thanks to the HTML-generator developed by ricocheting.com: [http://www.ricocheting.com/code/javascript/html-generator/image-slideshow HTML-Slideshow Generator]<br />
<br />
<br />
<br />
'''''Our 2012 Sponsors:'''''<br />
<br />
Ivana Chan & Family<br />
<br />
Sandra & Bill Hall<br />
<br />
[http://usa.autodesk.com/ Autodesk]<br />
<br />
[http://www.neb.com/nebecomm/default.asp New England Biolabs]<br />
<br />
[http://gastonday.org/ Gaston Day School]<br />
<br />
[http://markergene.com/ Marker Gene Technologies]<br />
<br />
<br />
<br />
<center><br />
<gallery widths=147px heights=130px perrow=5><br />
File:autodesklogo.jpg|[http://usa.autodesk.com/ Autodesk]<br />
File:nebGDS.jpg|[http://www.neb.com/nebecomm/default.asp New England Biolabs]<br />
File:Gaston_Day_School_logo.png|[http://gastonday.org/ Gaston Day School]<br />
File:Mgtlogoigem.jpg|[http://markergene.com/ Marker Gene Technologies]<br />
</gallery></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/SafetyTeam:Gaston Day School/Safety2012-10-02T02:31:17Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<h1><center>Safety</h1><br />
</html><br />
<br><br />
<br />
''''' 1. Would any project ideas raise safety issues in terms of ______? '''''<br />
::'''''A. Researcher Safety: ''''' <br />
::: As with any science experiment, there are risks involved with our project. Our team works with bleach which can cause irritation and a burning sensation if improperly exposed through inhalation, ingestion, or prolonged exposure to skin. However, any person is able to buy bleach from their local grocery store. Even so, we take care to prevent any issues. Also, our team works with ''E. coli'' K12. It is a version of ''E. coli'' that is widely studied and found to be very safe and practical for research. In fact, it is one of the most-studied bacteria of all time. ''E. coli'' K12 has no known survival mechanisms in the environment and has a very low risk of causing harm to organisms. ''E. coli'' K12 is in attenuated strain that is approved for use in high school classrooms. Even with antibiotic resistance, the amount of harm caused to most organisms would be minimal. The only real danger would be if a bacterium was able to conjugate with another type of bacteria that was more harmful and transfer its resistances. The chances of this happening are small due to the fact that the bacterium is held in a lab that most high school students do not enter (separate room that remains locked) and because proper safety precautions are followed relative to our biosafety level when inside this lab, including proper safety equipment and diligent hand washing.<br />
::'''''B. Public Safety: '''''<br />
:::There are not many reasonable threats to the safety and health of the public if the project were to be released. This is mainly because we are in the biosafety level (BSL) 1 category which limits what materials we can use. In saying this, bleach can be purchased by almost anyone at their local supermarket or grocery store. Therefore, the risk is no different than someone buying the bleach and using it as a household chemical. Also, ''E. coli'' K12 can be purchased by the public. Therefore, it is again low risk. As long as proper regulations are followed specific to their safety level, there is minimal risk. For the general public, the only way the bacterium may cause harm is through the spread of antibiotic resistance to chloramphenicol. The reason for this particular drug resistance is because this is what our team uses as a selective marker to attain the final product. It only becomes risky when this resistance is spread to more harmful bacteria.<br />
::'''''C. Environmental Safety? '''''<br />
:::There are no unreasonable, adverse effects associated with bleach being introduced into the environment. ''E. coli'' K12 has no known way of surviving in the environment and cannot produce spores. It is also not able to colonize the gut of an organism which makes it a very low environmental threat. From our machine, there are no real environmental threats. Again, this arises from working within BSL 1 and from the public having access to the same materials we use. Therefore, they are all relatively stable and safe. There is no real toxicity other than bleach, but it can be purchased at many stores and locations around the world and is only harmful from intentional misuse (ex. ingesting very large quantities, inhaling large amounts). Our machine itself is not pathogenic but if it is able to conjugate with other possibly pathogenic bacteria, then it may cause some degree of harm. If the bacterium is isolated then this will not happen and may be used safely.<br />
<br />
''''' 2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? '''''<br />
:::The BioBrick parts that we built do not raise any major safety concerns. A few minor risks are associated such as the potential conjugation and sharing of antibiotic resistance. However, the largest safety issue for this year’s project is in the testing of our sensors. We have sensors that test for heavy metal contaminants. Hence, we need to use heavy metals to test the functionality and sensitivity of our detectors. We used Lead Acetate (0.1 M) and Cadmium Nitrate (1 M), both of which are available for purchase from a science supply store. <br />
:''''' If yes, '''''<br />
::'''''A. Did you document these issues in the Registry?'''''<br />
::: We have not added parts to the registry at this point; proper documentation will be included when our BioBrick parts are added to the registry.<br />
::''''' B. How did you manage to handle the safety issue?'''''<br />
::: Extra care was taken when working with these materials such as the use of a fume hood and proper lab technique. Since we are in a high school setting, we must abide by all BSL 1 guidelines and regulations. This severely limits our available options. Many regulations are imposed upon our team; a few include: a sink must be present in the lab, controlled access (locked doors), no eating or drinking in lab area, decontamination of surfaces after lab work, and a lab supervisor. All work is supervised by our instructor, Ms. Anne Byford; she has a background in molecular genetics and trains all team members to ensure safety. She has also supervised and gone through the specific methods and processes we use to isolate, cut, ligate, and view the DNA used to create our bacterium. This also includes proper lab etiquette and how to use equipment such as a microcentrifuge, pipettes, and gel electrophoresis boxes. Proper safety technique is used when handling all materials. We treat everything as if it were hazardous. <br />
::''''' C. How could other teams learn from your experience?'''''<br />
::: Other teams could potentially learn from our experience through proper research on their biosafety level and its respective regulations. Also, teams could look at the materials used to test their machines, not just the construction process itself.<br />
''''' 3. Is there a local biosafety group, committee, or review board at your institution?'''''<br />
::'''''A. If “yes,” what does your local biosafety group think about your project?'''''<br />
<br />
:::At Gaston Day School we do not have a set of our own biosafety rules. In saying this, we do follow all of the regulations and rules from BSL 1 and operate under the supervision of our advisor, Ms. Anne Byford, who has much knowledge in the biological field.<br />
:::All of our iGEM projects at Gaston Day School are approved by the Head of the School, Dr. Richard Rankin, the Head of Middle and Upper School, Mr. Greg Rainey, and lastly the Board of Directors to ensure the safety of our team and school.<br />
<br />
::'''''B. If “no,” which specific biosafety rules or guidelines do you have to consider in your country?'''''<br />
:::The United States of America’s biosafety regulations are controlled by the CDC (Center for Disease Control and Prevention). More information can be found in the link below.<br />
<br />
<br />
<br />
''''' 4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices, and systems be made even safer through biosafety engineering?''''' <br />
::'''''A. '''''Safety is the most important part of the competition. Safety must be stressed upon and needs to be a priority in all labs. To help with this, each team and advisor should have to fill out a survey that measures the safety in their lab and also makes sure that they are staying within the regulations of their BSL. Also, photos should be submitted of the lab to show that they have the proper safety regulation and requirements.<br />
<br />
<br />
<br><br />
----<br />
<br><br />
<br />
<br />
''Citations:'' <br />
<br />
1. http://epa.gov/biotech_rule/pubs/fra/fra004.htm<br />
<br />
2. http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf pages 30-33.<br />
<br />
3. http://www.cdc.gov/biosafety/</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/SafetyTeam:Gaston Day School/Safety2012-10-02T02:31:08Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<h1><center>Safety</h1><br />
</html><br />
<br><br />
<br><br />
<br />
''''' 1. Would any project ideas raise safety issues in terms of ______? '''''<br />
::'''''A. Researcher Safety: ''''' <br />
::: As with any science experiment, there are risks involved with our project. Our team works with bleach which can cause irritation and a burning sensation if improperly exposed through inhalation, ingestion, or prolonged exposure to skin. However, any person is able to buy bleach from their local grocery store. Even so, we take care to prevent any issues. Also, our team works with ''E. coli'' K12. It is a version of ''E. coli'' that is widely studied and found to be very safe and practical for research. In fact, it is one of the most-studied bacteria of all time. ''E. coli'' K12 has no known survival mechanisms in the environment and has a very low risk of causing harm to organisms. ''E. coli'' K12 is in attenuated strain that is approved for use in high school classrooms. Even with antibiotic resistance, the amount of harm caused to most organisms would be minimal. The only real danger would be if a bacterium was able to conjugate with another type of bacteria that was more harmful and transfer its resistances. The chances of this happening are small due to the fact that the bacterium is held in a lab that most high school students do not enter (separate room that remains locked) and because proper safety precautions are followed relative to our biosafety level when inside this lab, including proper safety equipment and diligent hand washing.<br />
::'''''B. Public Safety: '''''<br />
:::There are not many reasonable threats to the safety and health of the public if the project were to be released. This is mainly because we are in the biosafety level (BSL) 1 category which limits what materials we can use. In saying this, bleach can be purchased by almost anyone at their local supermarket or grocery store. Therefore, the risk is no different than someone buying the bleach and using it as a household chemical. Also, ''E. coli'' K12 can be purchased by the public. Therefore, it is again low risk. As long as proper regulations are followed specific to their safety level, there is minimal risk. For the general public, the only way the bacterium may cause harm is through the spread of antibiotic resistance to chloramphenicol. The reason for this particular drug resistance is because this is what our team uses as a selective marker to attain the final product. It only becomes risky when this resistance is spread to more harmful bacteria.<br />
::'''''C. Environmental Safety? '''''<br />
:::There are no unreasonable, adverse effects associated with bleach being introduced into the environment. ''E. coli'' K12 has no known way of surviving in the environment and cannot produce spores. It is also not able to colonize the gut of an organism which makes it a very low environmental threat. From our machine, there are no real environmental threats. Again, this arises from working within BSL 1 and from the public having access to the same materials we use. Therefore, they are all relatively stable and safe. There is no real toxicity other than bleach, but it can be purchased at many stores and locations around the world and is only harmful from intentional misuse (ex. ingesting very large quantities, inhaling large amounts). Our machine itself is not pathogenic but if it is able to conjugate with other possibly pathogenic bacteria, then it may cause some degree of harm. If the bacterium is isolated then this will not happen and may be used safely.<br />
<br />
''''' 2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? '''''<br />
:::The BioBrick parts that we built do not raise any major safety concerns. A few minor risks are associated such as the potential conjugation and sharing of antibiotic resistance. However, the largest safety issue for this year’s project is in the testing of our sensors. We have sensors that test for heavy metal contaminants. Hence, we need to use heavy metals to test the functionality and sensitivity of our detectors. We used Lead Acetate (0.1 M) and Cadmium Nitrate (1 M), both of which are available for purchase from a science supply store. <br />
:''''' If yes, '''''<br />
::'''''A. Did you document these issues in the Registry?'''''<br />
::: We have not added parts to the registry at this point; proper documentation will be included when our BioBrick parts are added to the registry.<br />
::''''' B. How did you manage to handle the safety issue?'''''<br />
::: Extra care was taken when working with these materials such as the use of a fume hood and proper lab technique. Since we are in a high school setting, we must abide by all BSL 1 guidelines and regulations. This severely limits our available options. Many regulations are imposed upon our team; a few include: a sink must be present in the lab, controlled access (locked doors), no eating or drinking in lab area, decontamination of surfaces after lab work, and a lab supervisor. All work is supervised by our instructor, Ms. Anne Byford; she has a background in molecular genetics and trains all team members to ensure safety. She has also supervised and gone through the specific methods and processes we use to isolate, cut, ligate, and view the DNA used to create our bacterium. This also includes proper lab etiquette and how to use equipment such as a microcentrifuge, pipettes, and gel electrophoresis boxes. Proper safety technique is used when handling all materials. We treat everything as if it were hazardous. <br />
::''''' C. How could other teams learn from your experience?'''''<br />
::: Other teams could potentially learn from our experience through proper research on their biosafety level and its respective regulations. Also, teams could look at the materials used to test their machines, not just the construction process itself.<br />
''''' 3. Is there a local biosafety group, committee, or review board at your institution?'''''<br />
::'''''A. If “yes,” what does your local biosafety group think about your project?'''''<br />
<br />
:::At Gaston Day School we do not have a set of our own biosafety rules. In saying this, we do follow all of the regulations and rules from BSL 1 and operate under the supervision of our advisor, Ms. Anne Byford, who has much knowledge in the biological field.<br />
:::All of our iGEM projects at Gaston Day School are approved by the Head of the School, Dr. Richard Rankin, the Head of Middle and Upper School, Mr. Greg Rainey, and lastly the Board of Directors to ensure the safety of our team and school.<br />
<br />
::'''''B. If “no,” which specific biosafety rules or guidelines do you have to consider in your country?'''''<br />
:::The United States of America’s biosafety regulations are controlled by the CDC (Center for Disease Control and Prevention). More information can be found in the link below.<br />
<br />
<br />
<br />
''''' 4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices, and systems be made even safer through biosafety engineering?''''' <br />
::'''''A. '''''Safety is the most important part of the competition. Safety must be stressed upon and needs to be a priority in all labs. To help with this, each team and advisor should have to fill out a survey that measures the safety in their lab and also makes sure that they are staying within the regulations of their BSL. Also, photos should be submitted of the lab to show that they have the proper safety regulation and requirements.<br />
<br />
<br />
<br><br />
----<br />
<br><br />
<br />
<br />
''Citations:'' <br />
<br />
1. http://epa.gov/biotech_rule/pubs/fra/fra004.htm<br />
<br />
2. http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf pages 30-33.<br />
<br />
3. http://www.cdc.gov/biosafety/</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/PartsTeam:Gaston Day School/Parts2012-10-02T02:30:46Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br/><br />
<html><br />
<br><br />
<h1><center>Parts Submitted to the Registry</h1><br />
</html><br />
<br />
<br />
<groupparts>iGEM012 Gaston_Day_School</groupparts></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/TeamTeam:Gaston Day School/Team2012-10-02T02:30:12Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
__NOTOC__<br />
<br />
<!--- The Mission, Experiments ---><br />
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<h1><center>Who We Are</h1><br />
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Our team consists primarily of high school juniors and is based out of Gaston Day School, a preK-through 12 school in Gastonia, North Carolina. With Ms. Anne Byford's gudiance, we are taking part in our fourth of the iGEM competition.<br />
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'''Advisor:''': Anne Byford<br />
[[File:Teampic1.JPG|right|400px|caption]]<br />
'''Juniors:'''<br />
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*'''Dedicated Leader''': Steven Allen <br />
*'''Dedicated Co-Leader''': Parth Patel<br />
*'''A normal student...or am I?''': Gordon Ellison<br />
*'''Sam the Man''': Samuel DuBois<br />
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'''Sophmores:'''<br />
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*'''The Peasant''': Will Reiber<br />
*'''Young Grasshopper''': Audrey Weiss<br />
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<gallery widths=250px heights=250px perrow=3><br />
File:Tmsteven.jpg| <center>'''Steven Allen'''<br />
File:Tmparth.png|<center>'''Parth Patel'''<br />
File:Tmgordon.jpg|<center>'''Gordon Ellison'''<br />
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</gallery><br />
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<gallery widths=250px heights=250px perrow=3><br />
File:Tmsam.jpg|<center>'''Samuel DuBois'''<br />
File:Willpicuseonline.jpg|<center>'''Will Reiber'''<br />
File:Awgdsaudrey.JPG|<center>'''Audrey Weiss'''<br />
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<h1><center>What We Did</h1><br />
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We developed Cadmium, Arsenic, and Lead detectors by combining responsive promoters with Green Fluorescent Protein reporters.<br />
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<h1><center>Where We're From</h1><br />
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Founded in 1967, Gaston Day School, is a non-sectarian, college prearatory institution for grades<br />
Pre-K through 12. Throughout our history, nearly 100% of Gaston Day School's graduating<br />
classes have entered four-year colleges and universities immediately upon graduation. Located in<br />
Gastonia, North Carolina, the school serves communities in a five-county area: Gaston, Lincoln,<br />
Cleveland, Mecklenburg and York counties. Enrollment is approximately 500 students.<br />
Gaston Day School is accredited by the Southern Association of Colleges and Schools. The<br />
school is a member of the Southern Association of Independent Schools, the National<br />
Association of Independent Schools, and the North Carolina Association of Independent Schools.<br />
Each of the students on the team has completed (or is currently in) Honors Biology, Honors<br />
Chemistry, AP Biology, AP Chemistry, Honors Physics or some combination thereof. <br />
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<div class="center" style="width:auto; margin-center:auto; margin-right:auto;">'''Gaston Day School'''</div><br />
<html><br />
<iframe width="425" height="350" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="https://maps.google.com/maps?f=q&amp;source=s_q&amp;hl=en&amp;geocode=&amp;q=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;aq=1&amp;oq=Gaston+Day+School&amp;sll=35.170517,-79.860994&amp;sspn=7.621033,14.27124&amp;ie=UTF8&amp;hq=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;ll=35.201736,-81.141128&amp;spn=0.018235,0.032015&amp;t=p&amp;output=embed"></iframe><br /><small><a href="https://maps.google.com/maps?f=q&amp;source=embed&amp;hl=en&amp;geocode=&amp;q=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;aq=1&amp;oq=Gaston+Day+School&amp;sll=35.170517,-79.860994&amp;sspn=7.621033,14.27124&amp;ie=UTF8&amp;hq=Gaston+Day+School,+Gaston+Day+School+Road,+Gastonia,+NC&amp;ll=35.201736,-81.141128&amp;spn=0.018235,0.032015&amp;t=p" style="color:#0000FF;text-align:center">View Larger Map</a></small><br />
</html></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/ProjectTeam:Gaston Day School/Project2012-10-02T02:29:01Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
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<h1><center>Overall Project</h1><br />
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{|align="justify"<br />
|<br />
Heavy metal contaminants in water pose serious health problems; the lungs, liver, kidneys, blood, digestive system, and <br />
the nervous system are all affected by contamination. The Agency for Toxic Substances and Disease registry released a Priority List of Hazardous Substances (ASTDR). Heavy metals accounted for almost half of the top 10 substances; therefore, we have constructed a set of sensors that detects heavy metal contaminants in water. Our sensors provide an inexpensive, simple, and visual test for Arsenic (the number one substance from ASTDR’s list), Lead (number two), and Cadmium (number seven). One sensor paired a promoter responsive to both Cadmium and Arsenic with GFP as a reporter. Another was created for the detection of Lead and a third sensor was specific to cadmium. Use of the detector could potentially save lives around the world through early detection of the contamination.<br />
<br />
The kit will include all necessary components for running the test and then decontaminating the resulting growth to prevent release of the engineered bacteria into the environment. We will include a very simple mechanism for killing or denaturing the bacteria in our detector kit – bleach. Bleach is highly effective at killing bacteria and is readily available to the average person.<br />
|-<br />
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<h1><center>Project Details</h1><br />
</html><br />
We built the GFP Cadmium and Arsenic Sensors using NEB's BioBrick Construction Kit. We digested the promoter responsive to both Cadmium and Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoters were Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively. Schematics of the 2 processes are shown below.<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Constructsarscad.jpg|900px]]<br />
</div><br />
<br />
<br />
<br />
The same process was used to create our Lead Sensor. A lead responsive promoter (BBa_I721001) was paired with GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://openwetware.org/wiki/IGEM:Brown/2007 2007 Brown iGEM team]. The schematic process is shown below. <br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:leadconst.jpg|450px]]<br />
</div><br />
<br />
<br />
<html><br />
<br><br />
<h1><center>The Experiments</h1><br />
</html><br />
We tested out detectors (protocol can be found [https://static.igem.org/mediawiki/2012/d/da/2012GDS8-20.23.24.pdf here]) and measured the sensitivity using a spectrophotometer. We measured in Flu./OD which is the amount of fluorescence per OD (at 600nm). At first, we encountered many problems. We noticed that all of our dilutions, including the control were approximately the same. We realized that LB (Luria Broth, our growth medium) fluoresces naturally; hence, it masked the effects of the GFP. To solve this problem, we tried to run our tests using M9 Salts as a minimal growth medium. However, our bacteria were unable to grow in the medium. After this, our solution was to grow in LB, spin down the bacteria, then resuspend in PBS (phosphate buffer solution). Lastly, our spectrophotometer still continued to show the same fluorescence reading for most samples except the control. We then realized that the readings had maxed. Serial dilutions were used to determine accurate measurements. <br />
<br />
<br />
<html><br />
<br><br />
<h1><center>Results</h1><br />
</html><br />
<br />
Overall, our tests show that all 3 of our detectors worked properly, even the lead detector with the "non-functional" lead promoter.<br />
<br />
The graphs shown below represent our results (in Fluorescence per OD600) for the three detectors.<br />
<br/><br />
<br/><br />
<br/><br />
[[File:Arsgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Cadgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Leadgraph.jpg|center|400px|caption]]</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/ProjectTeam:Gaston Day School/Project2012-10-02T02:28:31Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Overall Project</h1><br />
</html><br />
{|align="justify"<br />
|<br />
Heavy metal contaminants in water pose serious health problems; the lungs, liver, kidneys, blood, digestive system, and <br />
the nervous system are all affected by contamination. The Agency for Toxic Substances and Disease registry released a Priority List of Hazardous Substances (ASTDR). Heavy metals accounted for almost half of the top 10 substances; therefore, we have constructed a set of sensors that detects heavy metal contaminants in water. Our sensors provide an inexpensive, simple, and visual test for Arsenic (the number one substance from ASTDR’s list), Lead (number two), and Cadmium (number seven). One sensor paired a promoter responsive to both Cadmium and Arsenic with GFP as a reporter. Another was created for the detection of Lead and a third sensor was specific to cadmium. Use of the detector could potentially save lives around the world through early detection of the contamination.<br />
<br />
The kit will include all necessary components for running the test and then decontaminating the resulting growth to prevent release of the engineered bacteria into the environment. We will include a very simple mechanism for killing or denaturing the bacteria in our detector kit – bleach. Bleach is highly effective at killing bacteria and is readily available to the average person.<br />
|-<br />
|<br />
|}<br />
<br />
<br />
<html><br />
<br><br />
<h1><center>Project Details</h1><br />
</html><br />
We built the GFP Cadmium and Arsenic Sensors using NEB's BioBrick Construction Kit. We digested the promoter responsive to both Cadmium and Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoters were Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively. Schematics of the 2 processes are shown below.<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Constructsarscad.jpg|900px]]<br />
</div><br />
<br />
<br />
<br />
The same process was used to create our Lead Sensor. A lead responsive promoter (BBa_I721001) was paired with GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://openwetware.org/wiki/IGEM:Brown/2007 2007 Brown iGEM team]. The schematic process is shown below. <br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:leadconst.jpg|450px]]<br />
</div><br />
<br />
<br />
=== The Experiments ===<br />
We tested out detectors (protocol can be found [https://static.igem.org/mediawiki/2012/d/da/2012GDS8-20.23.24.pdf here]) and measured the sensitivity using a spectrophotometer. We measured in Flu./OD which is the amount of fluorescence per OD (at 600nm). At first, we encountered many problems. We noticed that all of our dilutions, including the control were approximately the same. We realized that LB (Luria Broth, our growth medium) fluoresces naturally; hence, it masked the effects of the GFP. To solve this problem, we tried to run our tests using M9 Salts as a minimal growth medium. However, our bacteria were unable to grow in the medium. After this, our solution was to grow in LB, spin down the bacteria, then resuspend in PBS (phosphate buffer solution). Lastly, our spectrophotometer still continued to show the same fluorescence reading for most samples except the control. We then realized that the readings had maxed. Serial dilutions were used to determine accurate measurements. <br />
<br />
<br />
=== Results ===<br />
<br />
Overall, our tests show that all 3 of our detectors worked properly, even the lead detector with the "non-functional" lead promoter.<br />
<br />
The graphs shown below represent our results (in Fluorescence per OD600) for the three detectors.<br />
<br/><br />
<br/><br />
<br/><br />
[[File:Arsgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Cadgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Leadgraph.jpg|center|400px|caption]]</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/ProjectTeam:Gaston Day School/Project2012-10-02T02:28:10Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Overall Project</h1><br />
</html><br />
{|align="justify"<br />
|<br />
Heavy metal contaminants in water pose serious health problems; the lungs, liver, kidneys, blood, digestive system, and <br />
the nervous system are all affected by contamination. The Agency for Toxic Substances and Disease registry released a Priority List of Hazardous Substances (ASTDR). Heavy metals accounted for almost half of the top 10 substances; therefore, we have constructed a set of sensors that detects heavy metal contaminants in water. Our sensors provide an inexpensive, simple, and visual test for Arsenic (the number one substance from ASTDR’s list), Lead (number two), and Cadmium (number seven). One sensor paired a promoter responsive to both Cadmium and Arsenic with GFP as a reporter. Another was created for the detection of Lead and a third sensor was specific to cadmium. Use of the detector could potentially save lives around the world through early detection of the contamination.<br />
<br />
The kit will include all necessary components for running the test and then decontaminating the resulting growth to prevent release of the engineered bacteria into the environment. We will include a very simple mechanism for killing or denaturing the bacteria in our detector kit – bleach. Bleach is highly effective at killing bacteria and is readily available to the average person.<br />
|-<br />
|<br />
|}<br />
<br />
<br />
=== '''Project Details''' ===<br />
We built the GFP Cadmium and Arsenic Sensors using NEB's BioBrick Construction Kit. We digested the promoter responsive to both Cadmium and Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoters were Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively. Schematics of the 2 processes are shown below.<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Constructsarscad.jpg|900px]]<br />
</div><br />
<br />
<br />
<br />
The same process was used to create our Lead Sensor. A lead responsive promoter (BBa_I721001) was paired with GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://openwetware.org/wiki/IGEM:Brown/2007 2007 Brown iGEM team]. The schematic process is shown below. <br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:leadconst.jpg|450px]]<br />
</div><br />
<br />
<br />
=== The Experiments ===<br />
We tested out detectors (protocol can be found [https://static.igem.org/mediawiki/2012/d/da/2012GDS8-20.23.24.pdf here]) and measured the sensitivity using a spectrophotometer. We measured in Flu./OD which is the amount of fluorescence per OD (at 600nm). At first, we encountered many problems. We noticed that all of our dilutions, including the control were approximately the same. We realized that LB (Luria Broth, our growth medium) fluoresces naturally; hence, it masked the effects of the GFP. To solve this problem, we tried to run our tests using M9 Salts as a minimal growth medium. However, our bacteria were unable to grow in the medium. After this, our solution was to grow in LB, spin down the bacteria, then resuspend in PBS (phosphate buffer solution). Lastly, our spectrophotometer still continued to show the same fluorescence reading for most samples except the control. We then realized that the readings had maxed. Serial dilutions were used to determine accurate measurements. <br />
<br />
<br />
=== Results ===<br />
<br />
Overall, our tests show that all 3 of our detectors worked properly, even the lead detector with the "non-functional" lead promoter.<br />
<br />
The graphs shown below represent our results (in Fluorescence per OD600) for the three detectors.<br />
<br/><br />
<br/><br />
<br/><br />
[[File:Arsgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Cadgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Leadgraph.jpg|center|400px|caption]]</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/ProjectTeam:Gaston Day School/Project2012-10-02T02:27:58Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<html><br />
<br><br />
<h1><center>Overall Project</h1><br><br />
</html><br />
{|align="justify"<br />
|<br />
Heavy metal contaminants in water pose serious health problems; the lungs, liver, kidneys, blood, digestive system, and <br />
the nervous system are all affected by contamination. The Agency for Toxic Substances and Disease registry released a Priority List of Hazardous Substances (ASTDR). Heavy metals accounted for almost half of the top 10 substances; therefore, we have constructed a set of sensors that detects heavy metal contaminants in water. Our sensors provide an inexpensive, simple, and visual test for Arsenic (the number one substance from ASTDR’s list), Lead (number two), and Cadmium (number seven). One sensor paired a promoter responsive to both Cadmium and Arsenic with GFP as a reporter. Another was created for the detection of Lead and a third sensor was specific to cadmium. Use of the detector could potentially save lives around the world through early detection of the contamination.<br />
<br />
The kit will include all necessary components for running the test and then decontaminating the resulting growth to prevent release of the engineered bacteria into the environment. We will include a very simple mechanism for killing or denaturing the bacteria in our detector kit – bleach. Bleach is highly effective at killing bacteria and is readily available to the average person.<br />
|-<br />
|<br />
|}<br />
<br />
<br />
=== '''Project Details''' ===<br />
We built the GFP Cadmium and Arsenic Sensors using NEB's BioBrick Construction Kit. We digested the promoter responsive to both Cadmium and Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoters were Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively. Schematics of the 2 processes are shown below.<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Constructsarscad.jpg|900px]]<br />
</div><br />
<br />
<br />
<br />
The same process was used to create our Lead Sensor. A lead responsive promoter (BBa_I721001) was paired with GFP (BBa_I13401). However, the [http://partsregistry.org/Part:BBa_I721001 lead responsive promoter] was not known to function, according to the [http://openwetware.org/wiki/IGEM:Brown/2007 2007 Brown iGEM team]. The schematic process is shown below. <br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:leadconst.jpg|450px]]<br />
</div><br />
<br />
<br />
=== The Experiments ===<br />
We tested out detectors (protocol can be found [https://static.igem.org/mediawiki/2012/d/da/2012GDS8-20.23.24.pdf here]) and measured the sensitivity using a spectrophotometer. We measured in Flu./OD which is the amount of fluorescence per OD (at 600nm). At first, we encountered many problems. We noticed that all of our dilutions, including the control were approximately the same. We realized that LB (Luria Broth, our growth medium) fluoresces naturally; hence, it masked the effects of the GFP. To solve this problem, we tried to run our tests using M9 Salts as a minimal growth medium. However, our bacteria were unable to grow in the medium. After this, our solution was to grow in LB, spin down the bacteria, then resuspend in PBS (phosphate buffer solution). Lastly, our spectrophotometer still continued to show the same fluorescence reading for most samples except the control. We then realized that the readings had maxed. Serial dilutions were used to determine accurate measurements. <br />
<br />
<br />
=== Results ===<br />
<br />
Overall, our tests show that all 3 of our detectors worked properly, even the lead detector with the "non-functional" lead promoter.<br />
<br />
The graphs shown below represent our results (in Fluorescence per OD600) for the three detectors.<br />
<br/><br />
<br/><br />
<br/><br />
[[File:Arsgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Cadgraph.jpg|center|400px|caption]]<br />
<br/><br />
[[File:Leadgraph.jpg|center|400px|caption]]</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:27:14Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<h1><center>Outreach</h1><br><br />
</html><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br/><br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:27:04Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<html><br />
<br><br />
<h1><center>Outreach</h1><br><br />
</html><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br/><br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:25:03Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br/><br />
<br/><br />
<center>'''Outreach'''</center><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br/><br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:24:49Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br/><br />
<br/><br />
<center>'''''Outreach'''''</center><br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br/><br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:24:33Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<center>Outreach</center><br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br/><br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:24:21Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
===<center>Outreach</center>===<br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br/><br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:22:56Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br/><br />
<br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br/><br />
<br/><br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:22:15Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.JPG|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:21:16Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br/><br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!<br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:DNAnecklace.jpg|450px]]<br />
</div></div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:20:22Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures are shown below of "our poject" This "BioBrick" produces produces GFP in the presence of Arsenic.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:19:24Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures can be found below.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:18:56Z<p>Sallen: </p>
<hr />
<div>{{Template:Team:Gaston_Day_School/Templates/Top}}<br />
<br />
<br />
<br/><br />
<br/><br />
Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
<br />
We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures can be found below.<br />
<br/><br />
<br />
<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:Intlfestdemonst.jpg|950px]]<br />
</div><br />
<br/><br />
<br/><br />
For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
<br />
Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water &#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!</div>Sallenhttp://2012.igem.org/Team:Gaston_Day_School/OutreachTeam:Gaston Day School/Outreach2012-10-02T02:18:36Z<p>Sallen: </p>
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Gaston Day School hosts an event called the International Festival each year. For our outreach project, we set up a booth at the festival to educate people in how basic genetic engineering works using our iGEM project from last year as an example. The booth also functioned as a minor source of funding for our team. <br />
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We had two stations, one which had plasmid models for people to “build” their own BioBricks, and one where participants made a DNA necklace. For the model station, we had rings of brightly colored cardstock to represent reporters, promoters, and backbones. We asked people to come pick one of each part and then cut out and tape the parts together to mirror the process of digestion and ligation that our iGEM team used. The activity helped students understand what genetic engineering is through a simplified model. Pictures can be found below.<br />
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For our second booth we asked people to contribute one dollar to create “DNA necklaces” that consisted of isolated DNA from Wheat Germ. Each participant isolated wheat germ DNA for their own necklace. The demonstration allowed others to take part in the process of DNA isolation and also keep their own souvenir. This portion of the booth attracted many of the younger students, generating early interest in DNA and in the things one can do with DNA.<br />
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Here are the directions we gave each participant: <br/><br />
:1. Get a cup with 1 – 2 grams of raw wheat germ <br/><br />
:2. Add 20 ml hot water and swirl for about 2 min. <br/><br />
:3. Add 1 ml of detergent using a dropper <br/><br />
:4. Swirl to mix (be gentle) <br/><br />
:5. Slowly pour about 15 ml of cold 91% isopropanol onto the top of the water <br/><br />
&#160; &#160;<br />
::a. Make a layer of alcohol on top of the water <br/><br />
:6. DNA is the white stringy stuff that collects where the isopropanol and the water touch <br/><br />
:7. Use a dropper to collect the DNA and squirt it into a tube <br/><br />
:8. We will spin the DNA into a pellet at the bottom of the tube and add a string for the necklace!</div>Sallen