User contributions
From 2012.igem.org
(Latest | Earliest) View (newer 250 | older 250) (20 | 50 | 100 | 250 | 500)
- 03:25, 27 October 2012 (diff | hist) Team:Yale (top)
- 03:25, 27 October 2012 (diff | hist) Team:Yale
- 03:23, 27 October 2012 (diff | hist) N File:Team Yale.png (top)
- 02:28, 27 October 2012 (diff | hist) Team:Yale/Modeling (top)
- 02:27, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 02:22, 27 October 2012 (diff | hist) Team:Yale/Team (top)
- 02:22, 27 October 2012 (diff | hist) Team:Yale/Team
- 02:18, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 02:17, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Survey for off-target binding sites)
- 02:16, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 02:15, 27 October 2012 (diff | hist) File:MAGEfig3.png (uploaded a new version of "File:MAGEfig3.png") (top)
- 02:15, 27 October 2012 (diff | hist) File:MAGEfig2.png (uploaded a new version of "File:MAGEfig2.png") (top)
- 02:15, 27 October 2012 (diff | hist) File:MAGEfig1.png (uploaded a new version of "File:MAGEfig1.png") (top)
- 02:03, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 02:02, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 02:00, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:59, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:35, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:34, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:34, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:33, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:31, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Survey for off-target binding sites)
- 01:31, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:29, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:27, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:25, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:24, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:24, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:23, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:22, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:20, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 01:16, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:11, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:11, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:02, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 01:01, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:58, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:54, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:53, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:53, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:52, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:49, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:48, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:47, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Survey for off-target binding sites)
- 00:46, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:45, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:32, 27 October 2012 (diff | hist) Team:Yale/Modeling
- 00:31, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:30, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:25, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:25, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:23, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:17, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:14, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:11, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:04, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:03, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:03, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 00:01, 27 October 2012 (diff | hist) Team:Yale/Modeling (→Modeling the evolution of a population during MAGE)
- 23:59, 26 October 2012 (diff | hist) N File:MAGE model scheme.png (top)
- 23:52, 26 October 2012 (diff | hist) Team:Yale/Modeling
- 04:08, 4 October 2012 (diff | hist) Team:Yale/Attributions (top)
- 03:59, 4 October 2012 (diff | hist) Team:Yale
- 03:58, 4 October 2012 (diff | hist) Team:Yale/Modeling (→Survey for off-target binding sites)
- 03:58, 4 October 2012 (diff | hist) Team:Yale/Modeling (→Survey for off-target binding sites)
- 03:56, 4 October 2012 (diff | hist) Team:Yale/Project
- 03:55, 4 October 2012 (diff | hist) Team:Yale/Project (→Upcoming goals)
- 03:54, 4 October 2012 (diff | hist) Team:Yale/Project
- 03:54, 4 October 2012 (diff | hist) N File:Andrianafig1.png (top)
- 03:52, 4 October 2012 (diff | hist) N File:Jaefig1.png (top)
- 03:48, 4 October 2012 (diff | hist) Team:Yale/Project
- 03:41, 4 October 2012 (diff | hist) Team:Yale/Team (→Who we are)
- 03:40, 4 October 2012 (diff | hist) File:180px-Lightyear.jpg (uploaded a new version of "File:180px-Lightyear.jpg") (top)
- 03:40, 4 October 2012 (diff | hist) N File:180px-Lightyear.jpg
- 03:39, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 03:39, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 03:35, 4 October 2012 (diff | hist) Team:Yale/Attributions (→Acknowledgements)
- 03:35, 4 October 2012 (diff | hist) Team:Yale/Attributions (→Acknowledgements)
- 03:34, 4 October 2012 (diff | hist) Team:Yale/Attributions (→Acknowledgements)
- 03:29, 4 October 2012 (diff | hist) Team:Yale/Team (→Who we are)
- 03:29, 4 October 2012 (diff | hist) Team:Yale
- 03:28, 4 October 2012 (diff | hist) Team:Yale
- 03:27, 4 October 2012 (diff | hist) Team:Yale (→Projects)
- 03:27, 4 October 2012 (diff | hist) Team:Yale/Attributions (→Attributions for work done)
- 03:26, 4 October 2012 (diff | hist) Team:Yale/Notebook (→David Lim) (top)
- 03:25, 4 October 2012 (diff | hist) Team:Yale/Modeling
- 03:25, 4 October 2012 (diff | hist) Team:Yale/Modeling
- 03:24, 4 October 2012 (diff | hist) N File:MAGEhistogram.png (top)
- 03:19, 4 October 2012 (diff | hist) N File:Eqns2.png (top)
- 03:19, 4 October 2012 (diff | hist) N File:Eqns1.png (top)
- 03:18, 4 October 2012 (diff | hist) File:MAGEfig3.png (uploaded a new version of "File:MAGEfig3.png")
- 03:16, 4 October 2012 (diff | hist) N File:MAGEfig3.png
- 03:15, 4 October 2012 (diff | hist) N File:MAGEfig2.png
- 03:15, 4 October 2012 (diff | hist) N File:MAGEfig1.png
- 02:53, 4 October 2012 (diff | hist) Team:Yale/Modeling (→Algorithm optimization)
- 02:48, 4 October 2012 (diff | hist) Team:Yale
- 02:47, 4 October 2012 (diff | hist) File:Yale team.png (uploaded a new version of "File:Yale team.png") (top)
- 02:40, 4 October 2012 (diff | hist) Team:Yale/Project
- 02:11, 4 October 2012 (diff | hist) N File:Yeast Assembly Diagram.jpg (top)
- 02:05, 4 October 2012 (diff | hist) Team:Yale/Project
- 02:04, 4 October 2012 (diff | hist) N File:Experimental Methodology.jpg (top)
- 02:04, 4 October 2012 (diff | hist) N File:Msb200810-f1.jpg (top)
- 02:04, 4 October 2012 (diff | hist) N File:1000px-Yeast Assembly Diagram.jpg (top)
- 01:53, 4 October 2012 (diff | hist) Team:Yale/Project
- 01:52, 4 October 2012 (diff | hist) Team:Yale/Project (→Growth curves for Ac, Bs, and Ec)
- 01:51, 4 October 2012 (diff | hist) Team:Yale/Project (→Growth curves for Ac, Bs, and Ec)
- 01:51, 4 October 2012 (diff | hist) Team:Yale/Project (→Growth curves for Ac, Bs, and Ec)
- 01:50, 4 October 2012 (diff | hist) Team:Yale/Project (→Growth curves for Ac, Bs, and Ec)
- 01:48, 4 October 2012 (diff | hist) Team:Yale/Project
- 01:45, 4 October 2012 (diff | hist) N File:Bs growth curves.png (top)
- 01:44, 4 October 2012 (diff | hist) N File:EcNR2 growth curve.png (top)
- 01:44, 4 October 2012 (diff | hist) N File:Acb growth curves.png (top)
- 01:43, 4 October 2012 (diff | hist) Team:Yale/Project
- 01:39, 4 October 2012 (diff | hist) Team:Yale/Project
- 01:38, 4 October 2012 (diff | hist) Team:Yale/Project
- 01:38, 4 October 2012 (diff | hist) Team:Yale/Project
- 01:37, 4 October 2012 (diff | hist) Team:Yale/Project
- 01:33, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:33, 4 October 2012 (diff | hist) N File:David meet Jae.jpg (top)
- 01:31, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:31, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:30, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:30, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:30, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:30, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:29, 4 October 2012 (diff | hist) Team:Yale/Team (→Where we work)
- 01:28, 4 October 2012 (diff | hist) N File:David at work.jpg (top)
- 01:27, 4 October 2012 (diff | hist) N File:Lab space west.jpg (top)
- 01:25, 4 October 2012 (diff | hist) Team:Yale/Team
- 01:25, 4 October 2012 (diff | hist) Team:Yale/Team
- 01:22, 4 October 2012 (diff | hist) N File:West campus building.JPG (top)
- 01:20, 4 October 2012 (diff | hist) N File:Door logo.JPG (top)
- 00:54, 4 October 2012 (diff | hist) Team:Yale/Team
- 00:52, 4 October 2012 (diff | hist) Team:Yale/Notebook
- 00:49, 4 October 2012 (diff | hist) N Team:Yale/Notebook/Protocols/ (Created page with "==Preparation of Competent DH5-α cells== ===Materials:=== *LB Buffer *50 mL polypropylene tubes *TB Buffer *DMSO *DH5-α colonies or freshly thawed cells ====TB Buffer (DH5-...") (top)
- 00:46, 4 October 2012 (diff | hist) N File:IGEM Yale Protocols.pdf (top)
- 00:44, 4 October 2012 (diff | hist) Team:Yale/Notebook
- 00:40, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Notebook entries, sorted by wetlab user and date)
- 00:39, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:38, 4 October 2012 (diff | hist) N File:MAGE notebook 1..pdf (top)
- 00:38, 4 October 2012 (diff | hist) N File:Beta Homolog PCRs and Experiments..pdf (top)
- 00:37, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:36, 4 October 2012 (diff | hist) Team:Yale/Notebook
- 00:30, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:29, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:28, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:27, 4 October 2012 (diff | hist) N File:AFP group Protocols and Diary Part 2.pdf (top)
- 00:26, 4 October 2012 (diff | hist) N File:AFP diary 5.pdf (top)
- 00:25, 4 October 2012 (diff | hist) N File:RiAFP Diary Part 4.pdf (top)
- 00:24, 4 October 2012 (diff | hist) N File:AFP 2012 Diary 3.pdf (top)
- 00:18, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:16, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:14, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 00:06, 4 October 2012 (diff | hist) Team:Yale/Notebook (→Jae Seong No)
- 00:06, 4 October 2012 (diff | hist) N User:Jn323/7 August 2012 (Created page with "====Electroporation==== *Three strains used: BS168, BS1A833, BSPY79 *Filter-sterilize buffers =====Preparation of Cells===== *Check O/N growth :* Back-Dilution prepared until OD6...") (top)
- 00:05, 4 October 2012 (diff | hist) N User:Jn323/2 August 2012 (Created page with "====Kanomycin Plate Preparation==== *Autoclave two 500mL LB-Agar solutions *Add 0.5mL of stock kanomycin (50mg/mL) in one 500mL LB-Agar solution (final conc. 50ug/mL) *Add 0.2mL ...") (top)
- 00:05, 4 October 2012 (diff | hist) N User:Jn323/1 August 2012 (Created page with "====Electrophoresis==== =====Preparation of Cells===== *Check O/N growth :* OD600 reading - 1.8 :* Back-Dilution prepared until OD600 reading was between 0.4 and 0.8 '''Following...") (top)
- 00:05, 4 October 2012 (diff | hist) N User:Jn323/31 July 2012 (Created page with "====Preparation for Electroporation==== *Check overnight growth :*All grew (both plates and liquid cultures) except BS PY79 :*Error made in growth - BS PY79 NOT resistant to Spc;...") (top)
- 00:04, 4 October 2012 (diff | hist) N User:Jn323/30 July 2012 (Created page with "====Preparation for Electroporation==== *Inoculate cells into 10mL of LB media (scrape glycerol stock with metal loop and place into LB solution in culture tube) :*ADP1, EcNR2, B...") (top)
- 00:04, 4 October 2012 (diff | hist) N User:Jn323/24 July 2012 (Created page with "====PCR - Amplification of Kan cassette and RiAFP cassette==== ====Gel Verification & Imaging==== ====Gel Extraction====") (top)
- 00:04, 4 October 2012 (diff | hist) N User:Jn323/23 July 2012 (Created page with "====PCR - Amplification of Kan cassette and RiAFP cassette==== ====Gel Verification & Imaging====") (top)
- 00:04, 4 October 2012 (diff | hist) N User:Jn323/20 July 2012 (Created page with "====Suspension of Primers==== ====Primer Dilution Stock====") (top)
- 00:03, 4 October 2012 (diff | hist) N User:Jn323/18 July 2012 (Created page with "====Primer Confirmation / Resuspension====") (top)
- 00:03, 4 October 2012 (diff | hist) N User:Jn323/17 July 2012 (Created page with "====Preparation of EcNR2====") (top)
- 00:03, 4 October 2012 (diff | hist) N User:Jn323/16 July 2012 (Created page with "====Changes to Primers====") (top)
- 00:03, 4 October 2012 (diff | hist) N User:Jn323/11 July 2012 (Created page with "====Primer Designing====") (top)
- 00:02, 4 October 2012 (diff | hist) N User:Jn323/10 July 2012 (Created page with "====Reconstruction of Plasmid pSB1AK8-eGFP-TEV-RiAFP====") (top)
- 00:02, 4 October 2012 (diff | hist) N User:Jn323/9 July 2012 (Created page with "====Preparation of LB Agar Plates====") (top)
- 00:02, 4 October 2012 (diff | hist) N User:Jn323/6 July 2012 (Created page with "====Origami Cell Antibiotic Test====") (top)
- 00:01, 4 October 2012 (diff | hist) N User:Jn323/5 July 2012 (Created page with "====Serial Dilutions==== *Grow overnight culture of Normal Origami cells, Normal Origami cells exposed to IPTG, Transformed Origami cells exposed to IPTG :*Grown in ~5mL LB media...") (top)
- 00:01, 4 October 2012 (diff | hist) N User:Jn323/2 July 2012 (Created page with "====PCR (Site-Directed Mutagenesis)====") (top)
- 00:01, 4 October 2012 (diff | hist) N User:Jn323/26 June 2012 (Created page with "====Freeze-Thaw Assay, 1-hour cycles==== =====IPTG Induction===== *Scrape off 4-5 colonies of transformed origami cells and untransformed origami cells from respective plates cul...") (top)
- 00:00, 4 October 2012 (diff | hist) N User:Jn323/25 June 2012 (Created page with "====Cell Transformation==== *Thaw Origami cells on ice *Aliquot 50uL of competent cells into two pre-chilled 15mL culture tubes *Add DNA (1-5ng in 1-5uL) to one of the culture tu...") (top)
- 00:00, 4 October 2012 (diff | hist) N User:Jn323/22 June 2012 (Created page with "====Freeze-Thaw Assay #1, 24-hour cycle==== =====IPTG Induction===== *Scrape off 4-5 colonies of transformed origami cells and untransformed origami cells from respective plates ...") (top)
- 23:59, 3 October 2012 (diff | hist) Team:Yale/Notebook (→Jae Seong No)
- 23:57, 3 October 2012 (diff | hist) N User:Andriana/21 June 2012 (Created page with "====I. Started culture with the only plate showing bacterial growth – mutants 15+16====") (top)
- 23:57, 3 October 2012 (diff | hist) N User:Andriana/20 June 2012 (Created page with "====I. Finished the presentation powerpoint==== ====II. Continued freeze-thaw selection pressure tests for MAGE including plating of solution samples==== ====III. Measured approx...") (top)
- 23:56, 3 October 2012 (diff | hist) N User:Andriana/19 June 2012 (Created page with "====I. Transformed DH5-α cells using PCR reactions 7+8, 9+10, 15+16, and 29+30==== ====II. Plated the four transformed samples==== ====III. Continued record-keeping and worked o...") (top)
- 23:56, 3 October 2012 (diff | hist) User:Andriana/18 June 2012 (top)
- 23:55, 3 October 2012 (diff | hist) N User:Andriana/17 June 2012 (Created page with "====I. Finished concentration and dilution calculations==== ====II. Made all diluted (25 mM) primer solutions==== ====III. Ran a 7-8 mutation PCR, but forgot to set melting tempe...") (top)
- 23:55, 3 October 2012 (diff | hist) N User:Andriana/18 June 2012 (Created page with "====I. Finished concentration and dilution calculations==== ====II. Made all diluted (25 mM) primer solutions==== ====III. Ran a 7-8 mutation PCR, but forgot to set melting tempe...")
- 23:54, 3 October 2012 (diff | hist) N User:Andriana/16 June 2012 (Created page with "====I. Green Experiment Results:==== 12 hours after plating – about equal, abundant colony growth all across except for treatment D (no colonies): [[File:Andriana_Notebook_7...") (top)
- 23:54, 3 October 2012 (diff | hist) N User:Andriana/15 June 2012 (Created page with "====I. Meeting with Kaury Kucera about primer designs:==== * Confirmed findings about primer design for site-directed mutagenesis as follows: :# Mutations should be made closer...") (top)
- 23:53, 3 October 2012 (diff | hist) N User:Andriana/14 June 2012 (Created page with "====I. Assay Continued==== <ol start="7"> <li>After being frozen for 24 hours, BL 21 cell samples (normal and transformed – normal on plain agar plates and transformed on amp...") (top)
- 23:53, 3 October 2012 (diff | hist) N User:Andriana/13 June 2012 (Created page with "====I. 24 Hour Assay Continued==== <ol start="3"> <li>Centrifuged out pellets of all 4 cell samples grown overnight (all reached the stationary phase due to significant murkines...") (top)
- 23:53, 3 October 2012 (diff | hist) N User:Andriana/12 June 2012 (Created page with "*<span style="color:#FF0000">Ampicillin</span> and <span style="color:#00CC00">kanamycin</span> plates with transformed BL21 and origami cells had bacterial growth, but control p...") (top)
- 23:52, 3 October 2012 (diff | hist) N User:Andriana/11 June 2012 (Created page with "====I. Continued designing primers==== ====II. Rechecked primers==== ====III. More autoclaving of glassware==== ====IV. Miniprepped DH5-α cells grown from our transformation====...") (top)
- 23:52, 3 October 2012 (diff | hist) N User:Andriana/10 June 2012 (Created page with "====I. Started growing a batch of newly transformed DH5-α cells==== ====II. Continued designing primers==== <br>") (top)
- 23:52, 3 October 2012 (diff | hist) N User:Andriana/9 June 2012 (Created page with "====I. Continued designing primers==== ====II. Miniprepped the rest of RiAFP+GFP DH5-α cells==== ====III. Ordered some primers==== <br>") (top)
- 23:51, 3 October 2012 (diff | hist) N User:Jn323/8 June 2012 (Created page with "====I. Began growing stock RiAFP+GFP DH5-α cells (dissolve some scraped cells in 10 mL LB medium with 10 microliters of both <span style="color:#FF0000">ampicillin</span> and <s...") (top)
- 23:51, 3 October 2012 (diff | hist) N User:Andriana/7 June 2012 (Created page with "====I. Continued transformation of BL21 cells==== ====II. Continued and finished Transformation of DH5-α cells:==== ''NOTE: Conduct all procedures aseptically, sterilizing the...") (top)
- 23:51, 3 October 2012 (diff | hist) N User:Andriana/6 June 2012 (Created page with "====I. Began growing DH5-α cells in a medium==== # Dilute one colony scraped from an agar plate in 5 mL of LB in a 25 or 50 mL flask and incubate overnight in a shaker at 37C (...") (top)
- 23:50, 3 October 2012 (diff | hist) N User:Andriana/5 June 2012 (Created page with "====I. Plated DH5-α cells on plain agar plate (making competent DH5-α cells)==== ''Note: Instead of plating, growth in a culture flask can be begun directly'' # Have a plain a...") (top)
- 23:50, 3 October 2012 (diff | hist) N User:Andriana/4 June 2012 (Created page with "====I. Made necessary purchases==== ====II. Made antibiotic solutions (use sterile equipment):==== =====<span style="color:#FF0000">Ampicillin</span> Stock:===== # <span style...") (top)
- 23:49, 3 October 2012 (diff | hist) N User:DrJones1935/2 June 2012 (Created page with "====I. Unpacked==== ====II. Made plain agar plates:==== :Protocol: ::# Agar– 15 g ::# LB broth – 25g (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) ::# DI water – 1L ::# C...") (top)
- 23:49, 3 October 2012 (diff | hist) N User:Andriana/1 June 2012 (Created page with "====I. Unpacked==== <br>") (top)
- 23:49, 3 October 2012 (diff | hist) Team:Yale/Notebook (→Andriana Lebid)
- 23:43, 3 October 2012 (diff | hist) Team:Yale/Notebook (→David Lim)
- 23:41, 3 October 2012 (diff | hist) Team:Yale/Notebook (→David Lim)
- 23:40, 3 October 2012 (diff | hist) N User:DrJones1935/2 October 2012 (Created page with "====I. Check Overnight Liquid Cultures==== =====RESULTS:===== {| class="wikitable" |- ! Sample !! Kan50 !! Kan + Cm37 !! Kan + Tet15 !! IPTG (0.5 mM) + Kan !! IPTG + Kan + Cm !...") (top)
- 23:40, 3 October 2012 (diff | hist) N User:DrJones1935/1 October 2012 (Created page with "====I. Re-check plates that were allowed to grow more overnight==== =====RESULTS:===== No appreciable change in E. coli plates. 2 blue colonies developed on the ADP1 1 plate and...") (top)
- 23:40, 3 October 2012 (diff | hist) N User:DrJones1935/30 September 2012 (Created page with "====I. Check Transformation Plates for Colonies==== =====RESULTS:===== *''B. subtilis'' PY79 - no colonies on any plates *''E. coli'' MachI {| class="wikitable" |- ! Plate !! ...")
- 23:39, 3 October 2012 (diff | hist) N User:DrJones1935/29 September 2012 (Created page with "====I. Inoculate Liquid Cultures for Transformation==== *For MachI, ADP1, and PY79 cultures, inoculate 10 mL of LB with 100 uL of overnight cultures *For EcNR1 and EcNR2ΔlacZ, ...") (top)
- 23:39, 3 October 2012 (diff | hist) N User:DrJones1935/10 September 2012 (Created page with "====I. Measure the Concentration of Miniprepped Plasmids==== *Bring samples and Buffer EB bottle upstairs to Take3 plate reader *Add 2 uL of each sample according to the followi...")
- 23:39, 3 October 2012 (diff | hist) N User:DrJones1935/6 September 2012 (Created page with "====I. Check Overnight Cultures for Growth==== *RESULTS: All cultures showed growth. ====II. AGE of PCR Samples==== *'''Make Gel:''' :*Measure out 0.4 g of agarose and add to...") (top)
- 23:38, 3 October 2012 (diff | hist) N User:DrJones1935/5 September 2012 (Created page with "====I. Check Old Plates for Growth==== *RESULTS: There was XL1-Blue colony growth on Kan50 plates from 15 Aug 2012 that was not present on controls for the 1:1 (~4 colonies) and...") (top)
- 23:38, 3 October 2012 (diff | hist) N User:DrJones1935/15 August 2012 (Created page with "====I. Measure the Concentration of Extracted DNA==== *Bring samples and water upstairs to Take3 plate reader *Add 2 uL of each sample according to the following chart: {| class...")
- 23:37, 3 October 2012 (diff | hist) N User:DrJones1935/14 August 2012 (Created page with "====I. Check Plates for Growth==== ''RESULTS:'' Growth on positive controls was a large number of colonies but not a lawn as expected. No growth on any of the <span style="colo...") (top)
- 23:37, 3 October 2012 (diff | hist) N User:DrJones1935/13 August 2012 (Created page with "====I. Have Jason Repeat Experiment from Saturday with 10, 20, and 30 ug/mL Tetracycline Plates==== ====II. Design Primers for pBAV1K to Check Orientation of Beta Homolog Librar...") (top)
- 23:36, 3 October 2012 (diff | hist) N User:DrJones1935/11 August 2012 (Created page with "====I. Ligate Digested Plasmid and Digested Cassette Together==== *1:1 vector:insert ratio - Mix: :*0.34 uL water :*2 uL 10X T4 Buffer :*6 uL plasmid DNA (~0.05 pmol) :*10.66 uL...") (top)
- 23:36, 3 October 2012 (diff | hist) N User:DrJones1935/10 August 2012 (Created page with "====I. Digest pBAV1K-T5-gfp Plasmid and Cassette with XbaI and PstI==== *Mix (plasmid): :*37.5 uL water :*5 uL pBAV1K-T5-gfp DNA (~1 ug at 200 ng/uL) :*0.5 uL 100x BSA :*5 uL 10...")
- 23:35, 3 October 2012 (diff | hist) N User:DrJones1935/9 August 2012 (Created page with "====I. Prepare for Gel Extraction of Cassette==== *'''Make Gel:''' :*Measure out 0.40668 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and swirl to mix...")
- 23:35, 3 October 2012 (diff | hist) N User:DrJones1935/8 August 2012 (Created page with "====I. Prepare for Gel Extraction of LacZ Product from HF2==== *'''Make Gel:''' :*Measure out 0.40175 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and...")
- 23:34, 3 October 2012 (diff | hist) N User:DrJones1935/7 August 2012 (Created page with "====I. AGE of PCR Samples==== *'''Make Gel:''' :*Measure out 0.50131 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and swirl to mix :*Cover flask with ...") (top)
- 23:33, 3 October 2012 (diff | hist) N User:DrJones1935/6 August 2012 (Created page with "====I. AGE of PCR Samples==== *'''Make Gel:''' :*Measure out 0.50131 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and swirl to mix :*Cover flask with ...") (top)
- 23:33, 3 October 2012 (diff | hist) N User:DrJones1935/5 August 2012 (Created page with "====I. AGE of PCR Samples==== *'''Make Gel:''' :*Measure out 0.50325 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and swirl to mix :*Cover flask with ...") (top)
- 23:32, 3 October 2012 (diff | hist) N User:DrJones1935/4 August 2012 (Created page with "====I. AGE of PCR Samples==== *'''Make Gel:''' :*Measure out 0.45035 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and swirl to mix '''(0.9% agarose ge...") (top)
- 23:32, 3 October 2012 (diff | hist) N User:DrJones1935/3 August 2012 (Created page with "====I. AGE of PCR Samples==== *'''Make Gel:''' :*Measure out 0.40136 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and swirl to mix '''(0.8% agarose ge...") (top)
- 23:30, 3 October 2012 (diff | hist) N User:DrJones1935/2 August 2012 (Created page with "====I. Prepare for Gel Extraction of a PCR Product from 27 Jul 2012==== *'''Make Gel:''' :*Measure out 1.00721 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE ...")
- 23:29, 3 October 2012 (diff | hist) N User:DrJones1935/1 August 2012 (Created page with "====I. Order Beta Homolog Primers from Keck==== *Primers were ordered with the name/sequence in the following table: {| class="wikitable" |- ! Name !! Tm (Tm overhang/Tm anneal)...") (top)
- 23:29, 3 October 2012 (diff | hist) N User:DrJones1935/30 July 2012 (Created page with "====I. Prepare for Gel Extraction of a PCR Product from 27 Jul 2012==== *'''Make Gel:''' :*Measure out 0.75 g of agarose and add to a 250 mL E-flask :*Add 75 mL of 0.5x TBE buf...")
- 23:28, 3 October 2012 (diff | hist) N User:DrJones1935/29 July 2012 (Created page with " ====I. AGE of PCR Samples==== *'''Make Gel:''' :*Measure out 0.5 g of agarose and add to a 250 mL E-flask :*Add 50 mL of 0.5x TBE buffer and swirl to mix :*Cover flask with a ...") (top)
- 23:28, 3 October 2012 (diff | hist) N User:DrJones1935/27 July 2012 (Created page with "====I. Cross-over PCR==== *'''Template Mix Specifications:''' {| class="wikitable" |- ! Template !! Size (bp) !! Amount Wanted (pmol) !! Amount Wanted (ng) !! Concentration (ng/...") (top)
- 23:27, 3 October 2012 (diff | hist) N User:DrJones1935/26 July 2012 (Created page with "====I. Send Samples for Sequencing==== *Mix samples according to Keck Guidelines {| class="wikitable" |- ! Sample Name !! Water!! DNA !! F primer !! R primer !! Barcode # |- | L...") (top)
- 23:27, 3 October 2012 (diff | hist) N User:DrJones1935/24 July 2012 (Created page with "====I. Order Sequencing Primers from Keck==== *Primers were ordered with the name/sequence in the following table: {| class="wikitable" |- ! Name !! Tm !! Length<br>(bp) !! %GC ...") (top)
- 23:27, 3 October 2012 (diff | hist) N User:DrJones1935/23 July 2012 (Created page with "====I. Run Diagnostic PCR on Sample 3==== *'''Mix Reagents:''' :*Mix according to the following table {| class="wikitable" |- ! !! A !! B !! C !! D !! E !! F |- ! Reagent !!...")
- 23:25, 3 October 2012 (diff | hist) N User:DrJones1935/21 July 2012 (Created page with "====I. PCR==== *'''Mix Reagents:''' :*Mix according to the following table, where 2 = LacZ, 3 = CAT*, 4 = tet<sup>r</sup>, 0.1-0.3 = +template, and 0.4 = -template ::*For exampl...") (top)
- 23:25, 3 October 2012 (diff | hist) User:DrJones1935/22 July 2012 (→I. PCR) (top)
- 23:23, 3 October 2012 (diff | hist) N User:DrJones1935/22 July 2012 (Created page with "====I. PCR==== *'''Mix Reagents:''' :*Mix according to the following table, where 2 = LacZ, 3 = CAT*, 4 = tet<sup>r</sup>, 0.1-0.3 = +template, and 0.4 = -template ::*For exampl...")
- 23:23, 3 October 2012 (diff | hist) N User:DrJones1935/19 July 2012 (Created page with "====I. Measure the Concentration of Miniprepped Plasmids==== *Bring samples and Buffer EB bottle upstairs to Take3 plate reader *Add 2 uL of each sample according to the followi...")
- 23:22, 3 October 2012 (diff | hist) N User:DrJones1935/18 July 2012 (Created page with "====I. Agarose Gel Electrophoresis (AGE) of PCR Samples==== *'''Make Gel:''' :*Measure out <strike>0.5 g</strike> 0.50497 g of agarose and add to a 250 mL E-flask :*Add 50 mL o...") (top)
- 23:22, 3 October 2012 (diff | hist) N User:DrJones1935/17 July 2012 (Created page with "====I. Dilute primers to 10 uM working stock==== *Mix: :*2 uL 200 uM primer stock :*38 uL sterile, DNAse-, RNAse-free dH<sub>2</sub>O *''Note: I think that the p5 primers got mi...") (top)
- 23:21, 3 October 2012 (diff | hist) N User:DrJones1935/16 July 2012 (Created page with "====I. Ordered Cross-over PCR Primers from Keck==== *Primers were ordered with the name/sequence in the following table: {| class="wikitable" |- ! Name !! Tm (Tm overhang/Tm ann...")
- 23:21, 3 October 2012 (diff | hist) N User:DrJones1935/15 July 2012 (Created page with "====I. Check Cultures for Growth==== ''RESULTS (~09:30)'': *Overnight cultures of plasmid strain pBAV1K-T5-gfp both grew robustly overnight. I will not centrifuge them together ...") (top)
- 23:19, 3 October 2012 (diff | hist) N User:DrJones1935/13 July 2012 (Created page with "====I. Check Plates for Growth==== *''RESULTS'': All overnight plates of ''B. subtilis'' showed growth, including the plates from two days ago left on the bench top. I have saved...") (top)
- 23:18, 3 October 2012 (diff | hist) User:DrJones1935/12 July 2012 (→I: Check WT B. subtilis Liquid Culture for Growth) (top)
- 23:17, 3 October 2012 (diff | hist) N User:DrJones1935/11 July 2012 (Created page with "====I. Check Plates and Cultures for Growth==== ''RESULTS (~09:45)'': *All plates showed growth except ''A. baylyi'' ADP1''ΔmutS''. ECNR2 plate was sparse, but had colonies. Al...") (top)
- 23:16, 3 October 2012 (diff | hist) N User:DrJones1935/10 July 2012 (Created page with "====I. Streak cells for upcoming transformation with pBAV1K-T5-gfp plasmid==== *Using sterilized inoculating loop, scrape top layer of ice of glycerol stock and streak cells ont...") (top)
- 23:15, 3 October 2012 (diff | hist) N User:DrJones1935/5 July 2012 (Created page with "====I. Ordered Cross-over PCR Primers from Keck==== *Primers were ordered with the name/sequence in the following table: {| class="wikitable" |- ! Name !! Tm (Tm overhang/Tm an...") (top)
- 23:14, 3 October 2012 (diff | hist) N User:DrJones1935/30 June 2012 (Created page with "====I. Check overnight cultures for growth==== *As of ~2:30 PM when I got in, both cultures were cloudy corresponding to saturation ====II. Make 60% glycerol stock from 100% st...") (top)
- 23:14, 3 October 2012 (diff | hist) N User:DrJones1935/29 June 2012 (Created page with "====I. Check culture and plate for overnight growth==== *''Results: No growth in the ECNR2 culture at 37 <sup>o</sup>C, confirming that these cultures will not grow at these tem...") (top)
- 23:13, 3 October 2012 (diff | hist) N User:DrJones1935/28 June 2012 (Created page with "====I: Inoculate ECNR2 Cultures from Natalie Ma's Plate(from 7 Jun 2012)==== *Inoculate one isolated colony into ~2 mL of LB media in sterile culture tube (x2) *P...") (top)
- 23:12, 3 October 2012 (diff | hist) Nm User:DrJones1935/27 June 2012 (Created page with "====I: Make 15% Glycerol Stocks (3 total) from Successful Overnight Culture==== *Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3) :*SIMD43 *Add 200 uL of...") (top)
- 23:12, 3 October 2012 (diff | hist) N User:DrJones1935/26 June 2012 (Created page with "====I. Inoculate Liquid Cultures for Cultures Which Failed to Grow==== *Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the ...") (top)
- 23:11, 3 October 2012 (diff | hist) N User:DrJones1935/25 June 2012 (Created page with "====I. Inoculate Liquid Cultures for Cultures Which Failed to Grow==== *Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the ...") (top)
- 23:11, 3 October 2012 (diff | hist) N User:DrJones1935/22 June 2012 (Created page with "====I: Make 15% Glycerol Stocks (15 total) from Successful Overnight Cultures==== *Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3 per culture) :*SIMD40 ...") (top)
- 23:11, 3 October 2012 (diff | hist) N User:DrJones1935/21 June 2012 (Created page with "====I: Make 15% Glycerol Stocks (42 total) from Successful Overnight Cultures==== *Chill an aliquot of sterile 60% glycerol on ice *Transfer 600 uL of each liquid culture to eac...") (top)
- 23:03, 3 October 2012 (diff | hist) N User:DrJones1935/20 June 2012 (Created page with "====I. Make 100 mg/ mL <span style="color:#FF0000">Ampicillin</span> Stock Solution (small) for Plates/Liquid Cultures==== *Weigh out ''(150 mg)'' 150.32 mg of <span style="colo...") (top)
- 23:02, 3 October 2012 (diff | hist) N User:DrJones1935/15 June 2012 (Created page with "''Note: Professor Isaacs brought samples of'' B. subtilis ''1A40 from the Breaker Lab. There was 1 plate and 1 liquid culture'' ====I: Make 15% Glycerol Stocks (4) from ''B. sub...") (top)
- 23:02, 3 October 2012 (diff | hist) N User:DrJones1935/12 July 2012 (Created page with "====I: Check WT B. subtilis Liquid Culture for Growth==== *RESULTS: Culture appears uniformly cloudy consistent with overnight growth. The glycerol stocks are viable.")
(Latest | Earliest) View (newer 250 | older 250) (20 | 50 | 100 | 250 | 500)