http://2012.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=500&target=Water&year=&month=
2012.igem.org - User contributions [en]
2024-03-29T05:51:30Z
From 2012.igem.org
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http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-27T04:01:36Z
<p>Water: </p>
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5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li 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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
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</ul><br />
</li><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/achievements&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/achievements&amp;action=history">History</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
</nav><!-- nav --><br />
<br />
<br />
<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
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<h1><p>Results</p></h1><br />
<h3>1. Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔG/L+18.6T-116.9 (1)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src="https://static.igem.org/mediawiki/2012/0/0e/Result-7.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting by parts is performed.<br />
We calculate the average error as follows:<br><br />
<img src="https://static.igem.org/mediawiki/2012/6/6d/Result-8.1.JPG" alt="" class="change" /> (2)<br><br />
Here Δe is the average error, n is the number of data , E(prediction) is the efficiency predicted by our model and E(measurement) is the efficiency measured in experiment.<br><br />
For Figure 1, the average error is 16%.<br />
<br />
<br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src="https://static.igem.org/mediawiki/2012/7/77/Result-9.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by our own experimental data</h3><br />
<br />
<p>We did experiment to measure the efficiency of terminators.Using the experimental data, we plot the predicted efficiency based on our model with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/a/a5/Result-10.png" alt="" class="change" /><br><br />
(Figure 2, diagram of validation using our experimental data)</p><br><br />
<p>For Figure 2, we also calculate the average error. using equation(2). The result is 18%.</p><br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 3 the interface of our web tool)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 4 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 5 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-27T03:59:24Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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.pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
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<h1><p>Results</p></h1><br />
<h3>1. Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔG/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src="https://static.igem.org/mediawiki/2012/0/0e/Result-7.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting by parts is performed.<br />
We calculate the average error as follows:<br><br />
<img src="https://static.igem.org/mediawiki/2012/6/6d/Result-8.1.JPG" alt="" class="change" /> (2)<br><br />
Here Δe is the average error, n is the number of data , E(prediction) is the efficiency predicted by our model and E(measurement) is the efficiency measured in experiment.<br><br />
For Figure 1, the average error is 16%.<br />
<br />
<br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src="https://static.igem.org/mediawiki/2012/7/77/Result-9.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by our own experimental data</h3><br />
<br />
<p>We did experiment to measure the efficiency of terminators.Using the experimental data, we plot the predicted efficiency based on our model with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/a/a5/Result-10.png" alt="" class="change" /><br><br />
(Figure 2, diagram of validation using our experimental data)</p><br><br />
<p>For Figure 2, we also calculate the average error. using equation(2). The result is 18%.</p><br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 3 the interface of our web tool)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 4 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 5 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-27T03:58:10Z
<p>Water: </p>
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-100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
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<h1><p>Results</p></h1><br />
<h3>1. Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔG/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src="https://static.igem.org/mediawiki/2012/0/0e/Result-7.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting by parts is performed.<br />
We calculate the average error as follows:<br><br />
<img src="https://static.igem.org/mediawiki/2012/6/6d/Result-8.1.JPG" alt="" class="change" /> (2)<br><br />
Here Δe is the average error, n is the number of data , E(prediction) is the efficiency predicted by our model and E(measurement) is the efficiency measured in experiment.<br><br />
For Figure 1, the average error is 16%.<br />
<br />
<br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src="https://static.igem.org/mediawiki/2012/7/77/Result-9.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by our own experimental data</h3><br />
<br />
<p>We did experiment to measure the efficiency of terminators.Using the experimental data, we plot the predicted efficiency based on formula (2) with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/a/a5/Result-10.png" alt="" class="change" /><br><br />
(Figure 2, diagram of validation using our experimental data)</p><br><br />
<p>For Figure 2, we also calculate the average error. using equation(2). The result is 18%.</p><br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 3 the interface of our web tool)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 4 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 5 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-27T03:54:08Z
<p>Water: </p>
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no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
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<h1><p>Results</p></h1><br />
<h3>1. Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔG/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src="https://static.igem.org/mediawiki/2012/0/0e/Result-7.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting by parts is performed.<br />
We calculate the average error as follows:<br><br />
<img src="https://static.igem.org/mediawiki/2012/6/6d/Result-8.1.JPG" alt="" class="change" /> (2)<br><br />
Here Δe is the average error, n is the number of data , E(prediction) is the efficiency predicted by our model and E(measurement) is the efficiency measured in experiment.<br><br />
For Figure 1, the average error is 16%.<br />
<br />
<br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src="https://static.igem.org/mediawiki/2012/7/77/Result-9.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by existing data</h3><br />
<br />
<p>We collect existing data of efficiency of terminators from literatures and databases except Biofab. Using these data, we plot the predicted efficiency based on formula (2) with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/a/a5/Result-10.png" alt="" class="change" /><br><br />
(Figure 2, diagram of validation using our experimental data)</p><br><br />
<p>For Figure 2, we also calculate the average error. using equation(2). The result is 18%.</p><br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 3 the interface of our web tool)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 4 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 5 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/File:Result-10.png
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2012-10-27T03:52:51Z
<p>Water: </p>
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Water
http://2012.igem.org/File:Result-9.JPG
File:Result-9.JPG
2012-10-27T03:50:58Z
<p>Water: </p>
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Water
http://2012.igem.org/File:Result-8.1.JPG
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2012-10-27T03:46:22Z
<p>Water: </p>
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Water
http://2012.igem.org/File:Result-7.png
File:Result-7.png
2012-10-27T03:42:04Z
<p>Water: </p>
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
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2012-10-27T03:26:09Z
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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
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<h1><p>Results</p></h1><br />
<h3>1, Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔG/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src=" https://static.igem.org/mediawiki/2012/d/db/Result-1.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting is<br />
performed and the fitting curve is<br><br />
E=1.4D+39.1 (2)<br><br />
The R^2 is 0.54.</p><br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/7/78/Result-2.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by existing data</h3><br />
<br />
<p>We collect existing data of efficiency of terminators from literatures and databases except Biofab. Using these data, we plot the predicted efficiency based on formula (2) with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well with correlation coefficient 0.8<br><br />
<img src=" https://static.igem.org/mediawiki/2012/4/48/Result-3.png" alt="" class="change" /><br><br />
(Figure 2, diagram of validation using our experimental data)</p><br />
<br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 3 the interface of our web tool)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 4 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 5 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction
Team:SUSTC-Shenzhen-B/lab introduction
2012-10-27T03:22:54Z
<p>Water: </p>
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<br />
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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
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/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
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$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
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var canvas = document.createElement("canvas"),<br />
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ctx.drawImage(this, 0, 0);<br />
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<!-- MAIN CONTENT --><br />
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<br />
<article><br />
<h1><p>Introduction to the Lab Work</p></h1><br />
<br />
<br />
<h3 class="STYLE10">Objective</h3><br />
<p>The goal of our wet lab work is to validate our software prediction. Since the terminator efficiency data available in literatures is very limited, we designed 100 terminators through the knowledge of terminator structure, and designed an experiment to measure terminator efficiency. By analyzing experiment results, we can adjust the parameters in our model to make it more accurate.</p><br />
<br />
<h3 class="STYLE10">1, How to measure efficiency:</h3><br />
<br />
<img src="https://static.igem.org/mediawiki/2012/6/62/Project.introduction.3JPG.JPG" alt="" class="img_fl img_border" /><br />
<p>The figure above shows the plasmid designed to measure terminator efficiency. The terminator to be characterized would be flanked by gene sequences of two fluorescent proteins, GFP and RFP. By using flow cytometry, we could measure the fluorescence strength of GFP and RFP, so as to measure the terminator efficiency. </p><br />
<br />
The terminator efficiency of a terminator is calculated with the formula:<br />
<p> E = 1- S/T </p><br />
<p>where S is the fluorescence strength of GFP with terminator, and T is the fluorescence strength of GFP without terminator, which is the baseline strength.</p><br />
<br />
<br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/plasmid_construction"><b>2. Plasmid Construction</b></a><br />
<br />
<br />
<br />
<h3 class="STYLE10">3. Fluorescence strength quantification</h3><br />
<p>We used flow cytometer to measured the fluorescence strength. Laser was used to be the luminous source and then radiated perpendicular to the sample flow. Under the irradiation of laser, cells expressed fluorescent protein. We calculated the average expression level of those cells and got the average strength of GFP. We also used fluorescence microscope to take a picture of bacterial culture. </p><br />
<br />
<h3 class="STYLE10">4. Agreement with theoretical prediction</h3><br />
<P> According to our experiment measured terminator efficiencies and our software predicted d scores, we created a fit curve to relate all the data. The corelation coefficient is 0.8. So we have achieved a good agreement between experimental efficiency and TTEC predicted efficiency.</P><br />
<br />
<h3 class="STYLE10">5. Technical Standard</h3><br />
<P>Our terminator efficiency measurement protocol has been submitted to Biobrick foundation as a technical standard(BBF RFC 90).</P><br />
<br />
<br />
<br> <br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction
Team:SUSTC-Shenzhen-B/lab introduction
2012-10-27T03:22:07Z
<p>Water: </p>
<hr />
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<br />
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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
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/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
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var $$ = $(this);<br />
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var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
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$childUl.css(offsetDirection,emWidth);<br />
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minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
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};<br />
<br />
})(jQuery); // plugin code ends<br />
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<script type="text/javascript"><br />
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<br />
//Add Class Js to html<br />
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<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
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/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
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img = new Image();<br />
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document.body.appendChild(img);<br />
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img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
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canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
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ctx.drawImage(this, 0, 0);<br />
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png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
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<!-- MAIN CONTENT --><br />
<div id="outermain"><br />
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<section id="mainthecontent"><br />
<br />
<article><br />
<h1><p>Introduction to the Lab Work</p></h1><br />
<br />
<br />
<h3 class="STYLE10">Objective</h3><br />
<p>The goal of our wet lab work is to validate our software prediction. Since the terminator efficiency data available in literatures is very limited, we designed 100 terminators through the knowledge of terminator structure, and designed an experiment to measure terminator efficiency. By analyzing experiment results, we can adjust the parameters in our model to make it more accurate.</p><br />
<br />
<h3 class="STYLE10">1, How to measure efficiency:</h3><br />
<br />
<img src="https://static.igem.org/mediawiki/2012/6/62/Project.introduction.3JPG.JPG" alt="" class="img_fl img_border" /><br />
<p>The figure above shows the plasmid designed to measure terminator efficiency. The terminator to be characterized would be flanked by gene sequences of two fluorescent proteins, GFP and RFP. By using flow cytometry, we could measure the fluorescence strength of GFP and RFP, so as to measure the terminator efficiency. </p><br />
<br />
The terminator efficiency of a terminator is calculated with the formula:<br />
<p> E = 1- S/T </p><br />
where S is the fluorescence strength of GFP with terminator, and T is the fluorescence strength of GFP without terminator, which is the baseline strength.<br />
<br />
<br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/plasmid_construction"><b>2. Plasmid Construction</b></a><br />
<br />
<br />
<br />
<h3 class="STYLE10">3. Fluorescence strength quantification</h3><br />
<p>We used flow cytometer to measured the fluorescence strength. Laser was used to be the luminous source and then radiated perpendicular to the sample flow. Under the irradiation of laser, cells expressed fluorescent protein. We calculated the average expression level of those cells and got the average strength of GFP. We also used fluorescence microscope to take a picture of bacterial culture. </p><br />
<br />
<h3 class="STYLE10">4. Agreement with theoretical prediction</h3><br />
<P> According to our experiment measured terminator efficiencies and our software predicted d scores, we created a fit curve to relate all the data. The corelation coefficient is 0.8. So we have achieved a good agreement between experimental efficiency and TTEC predicted efficiency.</P><br />
<br />
<h3 class="STYLE10">5. Technical Standard</h3><br />
<P>Our terminator efficiency measurement protocol has been submitted to Biobrick foundation as a technical standard(BBF RFC 90).</P><br />
<br />
<br />
<br> <br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B
Team:SUSTC-Shenzhen-B
2012-10-27T03:12:30Z
<p>Water: </p>
<hr />
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<br />
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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
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* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
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* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
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*/<br />
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(function($){ // $ will refer to jQuery within this closure<br />
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minWidth : 9, // requires em unit.<br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
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<br />
<article><br />
<h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1><br />
<p><b>Welcome!!</b></p><br />
<p>We are sophomores at SUSTC. During this summer, we studied the efficiency of rho-independent <br />
transcription terminators using both <br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">theoretical modeling</a> <br />
and <br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experimental methods</a>. <br />
Here is the <br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">overview </a><br />
of our project.<br />
</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-26T23:04:42Z
<p>Water: </p>
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.widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li .smalldate{display:block;font-size:11px;font-style:italic;overflow:hidden}#footercontainer{background:#3f4432;-webkit-border-bottom-left-radius:7px;-webkit-border-bottom-right-radius:7px;-moz-border-radius-bottomleft:7px;-moz-border-radius-bottomright:7px;border-bottom-left-radius:7px;border-bottom-right-radius:7px}#footer{padding:25px 0 25px 0;color:#bbb9a1;font-weight:400;font-family:'Open Sans',sans-serif;font-size:13px}#footer a,#footer a:visited{color:#bbb9a1}<br />
<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
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speed : 'normal',<br />
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<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
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var $$ = $(this);<br />
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<br />
});<br />
};<br />
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$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
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jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
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jQuery(".toggle_container").hide();<br />
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document.body.appendChild(img);<br />
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img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
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ctx.drawImage(this, 0, 0);<br />
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document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
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img.src = pngurl;<br />
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/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
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if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
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<!-- MAIN CONTENT --><br />
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<section id="mainthecontent"><br />
<br />
<article><br />
<h1><p>Results</p></h1><br />
<h3>1, Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔ/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src=" https://static.igem.org/mediawiki/2012/d/db/Result-1.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting is<br />
performed and the fitting curve is<br><br />
E=1.4D+39.1 (2)<br><br />
The R^2 is 0.54.</p><br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/7/78/Result-2.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by existing data</h3><br />
<br />
<p>We collect existing data of efficiency of terminators from literatures and databases except Biofab. Using these data, we plot the predicted efficiency based on formula (2) with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well with correlation coefficient 0.8<br><br />
<img src=" https://static.igem.org/mediawiki/2012/4/48/Result-3.png" alt="" class="change" /><br><br />
(Figure 2, diagram of validation using our experimental data)</p><br />
<br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 3 the interface of our web tool)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 4 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 5 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-26T23:03:22Z
<p>Water: </p>
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src: local('Open Sans Semibold'), local('OpenSans-Semibold'), url(http://themes.googleusercontent.com/static/fonts/opensans/v6/MTP_ySUJH_bn48VBG8sNSnhCUOGz7vYGh680lGh-uXM.woff) format('woff');<br />
}<br />
@font-face {<br />
font-family: 'Open Sans';<br />
font-style: normal;<br />
font-weight: 400;<br />
src: local('Open Sans'), local('OpenSans'), url(http://themes.googleusercontent.com/static/fonts/opensans/v6/cJZKeOuBrn4kERxqtaUH3T8E0i7KZn-EPnyo3HZu7kw.woff) format('woff');<br />
}<br />
<br />
/* inner */<br />
.pagenavi{clear:both;padding:0}.pagenavi a,.pagenavi a:visited{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#727272}.pagenavi a:hover{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#b03121;text-decoration:none}.pagenavi .current{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#b03121}.pagenavi .pages{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px}#breadcrumb{text-align:right;margin-bottom:34px;clear:right}.post{margin-bottom:30px;padding-bottom:30px;clear:both;border-bottom:1px solid #dfddc7}.post img{padding:5px;background:#d2d0ba;width:590px;height:236px}.single{padding-bottom:20px}.postimg{margin-bottom:20px}.posttitle{margin:0 0 5px 0}.posttitle,.posttitle a{font-size:18px;color:#3f4432}.posttitle a:hover{text-decoration:none}.entry-text{overflow:hidden;padding-left:30px}.entry-text h2{padding-top:5px}.entry-content{margin:0;padding:12px 0 5px 0}.entry-date{float:left;text-align:center;font-size:18px;width:56px;height:48px;padding-top:8px;line-height:normal;-moz-border-radius:56px;-webkit-border-radius:56px;-khtml-border-radius:56px;border-radius:56px;background:#3f4432;color:#f1efda;display:block}.month{font-size:11px;display:block}.entry-utility{padding:20px 0 0;font-size:11px;font-style:italic}.single .entry-utility{padding:0 0 0}#comment h2{margin-bottom:25px;text-transform:uppercase;font-size:14px}.commentlist{list-style-type:none;padding:0;margin:0}.commentlist ol{list-style-type:none;padding:30px 0 0 90px;margin:0}.commentlist li{position:relative;padding:0 0 30px 0}.commentlist li li{position:relative;padding:0}.avatar{position:absolute;top:0;left:0}.fn{font-size:12px;font-style:normal}.tdate{padding-left:30px}.tdate,.reply{font-size:11px;color:#a6a6a6;font-style:italic}.comment-body{margin:0 0 0 90px;padding:20px;background:#f7f5e3}.comment-body p{margin-bottom:5px;margin-top:10px}.comment-body .more{padding:0 0}#commentform{margin-bottom:15px}#commentform label{display:block}#commentform .text-input{margin-bottom:8px;padding:8px 5px;vertical-align:middle}#commentform .textarea{margin-bottom:20px;padding:8px 5px;vertical-align:top;width:90%}.ts-gallery-img img{max-width:100%;padding:0;margin:0 auto}.ts-gallery-clear{clear:both;height:1px!important;line-height:1px!important;float:none!important}.ts-gallery ul{list-style-type:none;margin:0;padding:0;clear:both}.ts-gallery ul li.nomargin{margin-right:0}.ts-gallery-text{padding:3px 0;text-align:center}.ts-gallery-text h2{font-size:13px;color:#3f4432;margin-bottom:20px;font-weight:600;font-family:'Open Sans',sans-serif}.no-gallery-text{display:none}.ts-gallery-img{background:#d2d0ba;padding:5px;position:relative;line-height:normal}.ts-gallery-img:hover{background:#3f4432}.ts-gallery-img a.image{display:block;position:relative;overflow:hidden;line-height:normal}.ts-gallery-img a 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0;width:300px;float:left}.ts-gallery-col3 li.nomargin{margin-right:0}.ts-gallery-col3 h2{margin:0 0 5px 0!important}.ts-gallery-col3 .ts-gallery-img{width:290px;height:115px}.ts-gallery-col3 .ts-gallery-img img{width:290px;height:115px}.ts-gallery-col3 .ts-gallery-img a.image{width:290px;height:115px;display:block;position:relative}.ts-gallery-col3 .ts-gallery-img a .rollover{width:290px;height:115px}.ts-gallery-col3 .ts-gallery-text{padding:5px 0 0 0}form{margin:0;padding:0}fieldset{border:0}#contactform{margin:0 auto;position:relative}#contactform h4{text-transform:uppercase;margin-bottom:20px}#contactform label{display:block;width:100%;float:left;padding-bottom:5px}span.required{color:#888}span.error{color:red;text-align:left;font-size:11px;padding-bottom:15px;display:block}#contactform input.text-input{margin-bottom:15px;vertical-align:middle;width:60%;float:left;font-style:italic;padding:8px;color:#727272;font-size:11px}#contactform textarea{width:95%;float:left;font-style:italic;color:#727272;font-size:11px;font-family:Arial,Helvetica,sans-serif}#message{margin-left:0;font-weight:700;color:red}#message h2{}#message p{margin:6px 0}.note{color:#d45454}#contactform .button{cursor:pointer;margin-top:20px;clear:both;float:left}<br />
<br />
/* style */<br />
html,body{height:100%}body{font-family:Arial,Helvetica,sans-serif;font-size:12px;color:#727272;margin:0;padding:0;line-height:20px;background:#d6d4c0 url(/wiki/images/d/d7/Sustc-b1-pattern.png) repeat}*{margin:0;padding:0}:focus{outline:0}form{margin:0;padding:0}hr{border-width:0;height:1px;line-height:0;margin:30px 0;page-break-after:always;text-align:center;width:100%;clear:both;color:#dfddc7;background-color:#dfddc7}.clearfix:before,.clearfix:after{content:'\0020';display:block;overflow:hidden;visibility:hidden;width:0;height:0}.clearfix:after{clear:both}.clearfix{zoom:1}.clear,.clr{clear:both;display:block;overflow:hidden;visibility:hidden;width:0;height:0}h1,h2{margin-bottom:25px}h3,h4,h5,h6{margin-bottom:10px}h1{font-size:26px}h2{font-size:20px}h3{font-size:16px}h4{font-size:14px}h5{font-size:12px}h6{font-size:10px}h1,h2{font-weight:400;font-family:'Open Sans',sans-serif;color:#404631}h3,h4,h5,h6{font-weight:600;font-family:'Open 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0;padding:0 10px 0 50px;background-image:url(/wiki/images/3/3b/Sustc-b1-quote.png);background-repeat:no-repeat;background-position:0 0;clear:both;font-family:Georgia,Arial;font-style:italic;font-size:16px;line-height:22px}blockquote.left,blockquote.right{float:right;letter-spacing:0;margin-bottom:20px;margin-left:20px;margin-top:0;padding:0 20px 10px 60px;width:43%;background-position:0 0}blockquote.left{float:left;margin-left:0;margin-right:20px}blockquote p{margin-bottom:0;font-size:16px;line-height:20px}code{font-family:Verdana,Arial;letter-spacing:1px;margin:25px 0 25px 0;display:block;font-size:.9em;border-left:4px solid #cfcfcf;padding:15px 10px}#bodychild{width:1000px;margin:0 auto;padding:50px 0}#outercontainer{width:1000px}#outerheader{-webkit-border-top-left-radius:8px;-webkit-border-top-right-radius:8px;-moz-border-radius-topleft:8px;-moz-border-radius-topright:8px;border-top-left-radius:8px;border-top-right-radius:8px}#outerfooter{border-top:1px solid #e0e0e0;-webkit-border-bottom-left-radius:8px;-webkit-border-bottom-right-radius:8px;-moz-border-radius-bottomleft:8px;-moz-border-radius-bottomright:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px}#outerheader,#outerslider,#outerbeforecontent,#outermain{width:100%;margin:0 auto;background:#f1efda}#slidercontainer,#beforecontent,#maincontent,#footer{width:940px;margin:0 auto}header{padding:0}#logo.frontpage,#logo.frontpage:before{border:0!important}#logo{border-bottom:1px solid #dfddc7;margin-bottom:25px;padding-bottom:30px;position:relative;z-index:10}#logo:before{border-bottom:1px solid #dfddc7;bottom:2px;content:"";display:block;left:0;position:absolute;right:0;top:0;z-index:-1}#logo{margin:0 30px;padding:30px 0;text-align:center}#logo img{display:inline-block}#sn{list-style-type:none;margin:0;padding:25px 0 0 0;float:right}#sn li{list-style-type:none;margin:0;padding:0 10px 0 20px;display:inline;background:transparent}#sn 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ul{top:-999em}ul.sf-menu li li:hover ul,ul.sf-menu li li.sfHover ul{left:14em;top:-1px;margin-left:0}ul.sf-menu li li:hover li ul,ul.sf-menu li li.sfHover li ul{top:-999em}ul.sf-menu li li li:hover ul,ul.sf-menu li li li.sfHover ul{left:14em;top:-1px}.sf-menu ul li a{padding:10px 25px!important;text-transform:none;line-height:normal;font-size:13px!important;display:block;width:auto;white-space:no-wrap}.sf-menu ul li a:hover{}.sf-menu li ul{padding:0;-ms-filter:"alpha(Opacity=50)";filter:alpha(opacity=80);-moz-opacity:.8;-khtml-opacity:.8;opacity:.8}.sf-menu a.sf-with-ul{min-width:1px}.sf-sub-indicator{position:absolute;display:block;right:10px;top:1.05em;width:10px;height:10px;text-indent:-999em;overflow:hidden}.sf-menu li li{background:#404533;border-bottom:dotted 1px #707563;border-left:5px solid #404533}.sf-menu li li:hover{border-left:5px solid 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a#mycarousel-next{background:url(/wiki/images/8/8b/Sustc-b1-button-prevnext.png) no-repeat -12px 0;right:46px;bottom:58px;position:absolute}#outerbeforecontent{clear:both}#beforecontent{}#beforethecontent{}.box{float:left;margin-right:2px;width:33.16%;background:#e1dfc9;text-align:center;padding-bottom:28px}.box h2{color:#efeed9;font-weight:700;background:#3f4432;padding:15px 0}.box p{overflow:hidden;padding:0 30px}#outermain{padding:0}#maincontent{}#mainthecontent{padding:32px 0 40px 0}#t-content{width:600px;float:left}#t-content.positionright{float:right}#t-content.positionleft{float:left}.small{font-size:11px;font-style:italic;margin-bottom:5px;display:block;margin-top:-5px}form{margin:0;padding:0}input[type="text"],textarea,input[type="password"],select{font-size:12px;padding:7px;background:#ebe9d1;border:0;color:#727272;border:0}textarea{width:90%}select{font-size:11px;padding:4px 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5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li 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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded 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* http://www.gnu.org/licenses/gpl.html<br />
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*<br />
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*<br />
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<article><br />
<h1><p>Results</p></h1><br />
<h3>1, Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔ/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src=" https://static.igem.org/mediawiki/2012/d/db/Result-1.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting is<br />
performed and the fitting curve is<br><br />
E=1.4D+39.1 (2)<br><br />
The R^2 is 0.54.</p><br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/7/78/Result-2.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by existing data</h3><br />
<br />
<p>We collect existing data of efficiency of terminators from literatures and databases except Biofab. Using these data, we plot the predicted efficiency based on formula (2) with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well with correlation coefficient 0.8<br />
<img src=" https://static.igem.org/mediawiki/2012/4/48/Result-3.png" alt="" class="change" /><br><br />
(Figure 2, diagram of validation using our experimental data)</p><br />
<br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 4 the interface of our web tool)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 5 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 6 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-26T23:01:24Z
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<br />
/* prettyPhoto.css */<br />
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no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery 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no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px 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.pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/achievements&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/achievements&amp;action=history">History</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
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<h1><p>Results</p></h1><br />
<h3>1, Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔ/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src=" https://static.igem.org/mediawiki/2012/d/db/Result-1.png" alt="" class="change" /><br><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting is<br />
performed and the fitting curve is<br><br />
E=1.4D+39.1 (2)<br><br />
The R^2 is 0.54.</p><br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br><br />
<img src=" https://static.igem.org/mediawiki/2012/7/78/Result-2.JPG" alt="" class="change" /><br><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by existing data</h3><br />
<br />
<p>We collect existing data of efficiency of terminators from literatures and databases except Biofab. Using these data, we plot the predicted efficiency based on formula (2) with efficiency data (Fig 2). We found that our theoretical predication fit experiemental data quite well with correlation coefficient 0.8<br />
<img src=" https://static.igem.org/mediawiki/2012/4/48/Result-3.png" alt="" class="change" /><br />
(Figure 2, diagram of validation using our experimental data)</p><br />
<br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br><br />
(Figure 4 the interface of our web tool)<br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br><br />
(Figure 5 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br><br />
(Figure 6 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements
Team:SUSTC-Shenzhen-B/achievements
2012-10-26T23:00:11Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery 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no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px 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.pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/achievements&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/achievements&amp;action=history">History</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
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<article><br />
<h1><p>Results</p></h1><br />
<h3>1, Theoretical model of predicting efficiency of terminators</h3><br />
<br />
<p>The goal of theoretical model to predict the efficiency of a terminator from its<br />
nucleotide sequence. We use free energy methods to predict the secondary structure<br />
of terminator. A d score is defined as<br><br />
D=-96.6DΔ/L+18.6T-116.9 (2)<br><br />
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.<br />
</p><br />
<p>To get a relationship between d score and efficiency, we use the biofab data as<br />
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p><br />
<img src=" https://static.igem.org/mediawiki/2012/d/db/Result-1.png" alt="" class="change" /><br />
(Figure 1 diagram showing the correlation between d score and efficiency)<br />
<br />
<p>We found that d score and efficiency is positively correlated. Linear fitting is<br />
performed and the fitting curve is<br><br />
E=1.4D+39.1 (2)<br><br />
The R^2 is 0.54.</p><br />
<p>We proposed that terminators can be classified into three classes based on efficiency.<br />
<img src=" https://static.igem.org/mediawiki/2012/7/78/Result-2.JPG" alt="" class="change" /><br />
(Table.1 Table of efficiency and d score correspond to different class of terminators).<br />
<br />
<h3>2, Validating theoretical model by existing data</h3><br />
<br />
<p>We collect existing data of efficiency of terminators from literatures and databases except Biofab. Using these data, we plot the predicted efficiency based on formula (2) with efficiency data (Fig 2). We found that our theoretical predication fit experiemental data quite well with correlation coefficient 0.8<br />
<img src=" https://static.igem.org/mediawiki/2012/4/48/Result-3.png" alt="" class="change" /><br />
(Figure 2, diagram of validation using our experimental data)</p><br />
<br />
<h3>3, TTEC software and webtool</h3><br />
<p>We developed TTEC software to predict terminator efficiency, which is available at<br />
www.terminator efficiency.com (for our web tool)<br><br />
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)<br><br />
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br />
(Figure 4 the interface of our web tool)<br />
<img src=" https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br />
(Figure 5 the intrface of outr software)</p><br />
<h3>4, Database of terminator efficiency</h3><br />
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.<br />
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br />
(Figure 6 our database of terminators whose efficiency have been measured)</p><br />
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Water
http://2012.igem.org/File:Result-6.png
File:Result-6.png
2012-10-26T22:59:19Z
<p>Water: </p>
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Water
http://2012.igem.org/File:Result-5.png
File:Result-5.png
2012-10-26T22:57:12Z
<p>Water: </p>
<hr />
<div></div>
Water
http://2012.igem.org/File:Result-4.png
File:Result-4.png
2012-10-26T22:56:08Z
<p>Water: </p>
<hr />
<div></div>
Water
http://2012.igem.org/File:Result-3.png
File:Result-3.png
2012-10-26T22:53:47Z
<p>Water: </p>
<hr />
<div></div>
Water
http://2012.igem.org/File:Result-2.JPG
File:Result-2.JPG
2012-10-26T22:52:04Z
<p>Water: </p>
<hr />
<div></div>
Water
http://2012.igem.org/File:Result-1.png
File:Result-1.png
2012-10-26T22:47:23Z
<p>Water: </p>
<hr />
<div></div>
Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B
Team:SUSTC-Shenzhen-B
2012-10-26T21:32:21Z
<p>Water: </p>
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.pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
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<h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1><br />
<p><b>Welcome!!</b></p><br />
<p>We used both <br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm"> theoretical model</a> and<br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experiment</a> method.<br />
to study the rho-independent transcription terminators which regulate gene expression.</p><br />
<p>Hope our work is useful for the comprehensive study on terminator efficiency.</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B
Team:SUSTC-Shenzhen-B
2012-10-26T21:31:28Z
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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
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<article><br />
<h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1><br />
<p><h2>Welcome!!</h2></p><br />
<p>We used both <br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm"> theoretical model</a> and<br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experiment</a> method.<br />
to study the rho-independent transcription terminators which regulate gene expression.</p><br />
<p>Hope our work is useful for the comprehensive study on terminator efficiency.</p><br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B
Team:SUSTC-Shenzhen-B
2012-10-26T21:30:47Z
<p>Water: </p>
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.widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li .smalldate{display:block;font-size:11px;font-style:italic;overflow:hidden}#footercontainer{background:#3f4432;-webkit-border-bottom-left-radius:7px;-webkit-border-bottom-right-radius:7px;-moz-border-radius-bottomleft:7px;-moz-border-radius-bottomright:7px;border-bottom-left-radius:7px;border-bottom-right-radius:7px}#footer{padding:25px 0 25px 0;color:#bbb9a1;font-weight:400;font-family:'Open Sans',sans-serif;font-size:13px}#footer a,#footer a:visited{color:#bbb9a1}<br />
<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Results</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
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</ul><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B&action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B&action=history">History</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
</nav><!-- nav --><br />
<br />
<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
<div id="logo" class="frontpage"><a href="#"><img src="https://static.igem.org/mediawiki/2012/3/3a/Tihuan.JPG" alt=""</a></div><br />
<div class="clear"></div><br />
</header><br />
</div><br />
<!-- END HEADER --><br />
<!-- SLIDER --><br />
<div id="outerslider"><br />
<div id="slidercontainer"><br />
<section id="slider"><br />
<br />
<div id="feature_gallery"><br />
<div class="bigimg"><br />
<img src="https://static.igem.org/mediawiki/2012/f/f1/School.JPG" /><br />
<div class="slidedesc">South University of Science and Technology of China.</div><br />
</div><br />
<div class="bigimg"><br />
<img src="https://static.igem.org/mediawiki/2012/6/66/Went_to_bgi.JPG" alt="" class="change" /><br />
<div class="slidedesc">2012 SUSTC IGEM team members.</div><br />
</div><br />
<div class="bigimg"><br />
<img src="https://static.igem.org/mediawiki/2012/archive/7/75/20121022104002%21Slider.3.JPG" alt="" class="change" /><br />
<div class="slidedesc">Web Page of Transcriptional Terminator Efficiency Calculator.</div><br />
</div><br />
<div class="bigimg"><br />
<img src="https://static.igem.org/mediawiki/2012/a/a3/Sustc-lab.JPG" alt="" class="change" /><br />
<div class="slidedesc">Lab Work.</div><br />
</div><br />
<div class="bigimg"><br />
<img src="https://static.igem.org/mediawiki/2012/a/ab/Hupra-3.JPG" alt="" class="change" /><br />
<div class="slidedesc">Human Practice.</div> <br />
</div><br />
<div class="bigimg"><br />
<img src="https://static.igem.org/mediawiki/2012/f/fb/Ff-hk.JPG" alt="" class="change" /><br />
<div class="slidedesc">HK jamboree</div><br />
</div><br />
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<a href="#" id="mycarousel-prev"></a> <a href="#" id="mycarousel-next"></a> <br />
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<br />
</section><br />
</div><br />
</div><br />
<!-- END SLIDER --><br />
<br />
<!-- MAIN CONTENT --><br />
<div id="outermain"><br />
<div id="maincontent"><br />
<section id="mainthecontent"><br />
<br />
<article><br />
<h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1><br />
<p>Welcome!!We used both <br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm"> theoretical model</a> and<br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experiment</a> method.<br />
to study the rho-independent transcription terminators which regulate gene expression.</p><br />
<p>Hope our work is useful for the comprehensive study on terminator efficiency.</p><br />
<br />
</article><br />
<br />
<div class="clear"></div><br />
<div class="separator line"></div><br />
<div class="clear"></div><br />
</section><br />
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<div id="outerfooter"><br />
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<footer id="footer">South University of Science and Technology of China</footer><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro
Team:SUSTC-Shenzhen-B/SynBio.intro
2012-10-26T21:29:30Z
<p>Water: </p>
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5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li 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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
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</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
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</ul><br />
</li><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/SynBio.intro&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/SynBio.intro&amp;action=history">History</a></li><br />
</ul><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
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<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
</nav><!-- nav --><br />
<br />
<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
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<h1><p>Synthetic Biology - is it another Name for Genetic Engineering ?</p></h1><br />
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<P>People must hear the term, 'genetic engineering', however, synthetic biology seems to be new. Somebody wonders if this is a new name referring to genetic engineering.</P><br />
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<P>Synthetic biology which was a infant discipline emerged within this few years. It focused on devices assembling, system building and engineering methodology. Of course, it was similar to genetic engineering and advantaged most of the techniques of genetic engineering. But synthetic biology was a brand-new stuff which was the further development of genetic engineering.</P><br />
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<P>In terms of scale, conventional genetic engineering tried to transfer one or a few genes from one organelle to another, maybe with tiny modification on genes. Synthetic biology wants more, the ultimate aim of synthetic biology is to synthesize new life to meet our need. This kind of new life is nothing but artificial life. So at this point synthetic biology intersects with system biology. Since synthetic biology is young, it need to enrich the available devices and modules to build the system.</P><br />
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<P>Where do the devices and modules come from? This answer reveals other aspects of differences between synthetic biology and genetic engineering. Traditionally, if we wanted a fluorescence pig, we had to find the cDNA of fluorescence gene. If the genes we wanted were rare, maybe we needed to catch that creature by ourselves. The whole picture changed after the technology development of high-speed and cheap sequencing and inexpensive DNA synthesis. All we need to do is start computers and search for target genes in database, then make an order to let company to synthesize those genes for us. The name 'synthetic' came from this. </P><br />
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<P>Suppose we get the genes, the next step comes to constructing. Here, we shall see the last differences. Conventional genetic engineering connected the parts and tested the functions differently from cases to cases. Thanks to standardization, assembling in synthetic biology is as easy as playing with LEGO toys, the interface is well-designed so that the construction is so simple. Besides, abstraction, reliability, uniformity and reuse, the generic terms in engineering, are all introduced to synthetic biology. Therefore, synthetic biology turns to a field of multidiscipline. Experts with different backgrounds gather to make their efforts. Programmers write the code to calculate, mathematics find model to predict, Engineers consider about the hierarchy, biologists debug the system. Ironically, synthetic biology is more engineering than genetic engineering.</P><br />
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<P>Talking about hierarchy, let me make an analogy between synthetic biology and computer hardware. Proteins and genes are compared to resistance, capacity which are the bottom of the system. Upwards, biochemical reaction is the same as gates in electronics because both of them can function basically. If biochemical reactions form a network and gates form a network, we call them pathways and modules in synthetic biology and electrical engineering respectively. Moreover, there are computers and cells. </P><br />
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<P>The dream is so nice but the reality is so hard. There is still plenty of work to do to make synthetic biology to be a qualified engineering. Often, we don't even fully understand the devices we are using and the whole system is not under our fully control. Despite this, after all, a new world is in front of us, as a Polish geneticist wrote,' I am not concerned that we will run out of exciting and novel ideas, ... in the synthetic biology, in general.'</P><br />
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<h3>Posters Made by Ourselves</h3><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits
Team:SUSTC-Shenzhen-B/high.school.visits
2012-10-26T21:29:03Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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.pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/high.school.visits&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/high.school.visits&amp;action=history">History</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
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<h1><p>High School Visits</p></h1><br />
<p>After participating in the iGEM competition, all of us had an idea of what synthetic biology was and what it could do for human. We thought it was our responsibility to let the public know more about synthetic biology and have a rational understanding of it. So we went to three high schools to hold lectures. We hoped that those students would then introduce synthetic biology to others, just like Pay It Forward.</p><br />
<p>In the lecture, we introduced synthetic biology and iGEM. When introducing synthetic biology, we emphasized the idea of standardization. We also delivered an idea that synthetic biology is not creating life but creating life for practical purposes. What’s more, we showed students the application and research achievements of synthetic biology. After talking about synthetic biology, we introduced iGEM. We showed them some interesting project and invited them to participate in the competition.</p><br />
<p>In the Q&A time, those students asked many questions.Here we listed three kind of questions that most students asked:</p><br />
<p>1. questions about the safety and ethic issues.</p><br />
<p>2. questions about the difference between synthetic biology and genetic engineering</p><br />
<p>3. questions about iGEM competition</p><br />
<br />
<p>Pro He answered all the questions patiently based on his understanding of synthetic biology. Students clapped for his beautiful and scientific answers. After the lecture, many students showed interest in High School iGEM competition. We introduced details of iGEM competition to them and welcome them to our school to have some corporation. SUSTC-Shenzhen-A team made a handbook of synthetic biology. The biology club member in Shenzhen High School took more than 100 handbooks, saying that they want to introduce synthetic biology to others. Hearing this, we felt so happy because it was really like Pay It Forward.</p><br />
<p>We hoped that more and more people have an understanding of synthetic biology and participate in the field of synthetic biology.</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class
Team:SUSTC-Shenzhen-B/open.class
2012-10-26T21:28:40Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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.pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
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<h1><font face="Arial, Helvetica"><p>Open classes</p></font></h1><br />
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<h3 class="STYLE2"><font face="Arial, Helvetica">About our Open Classes </font></h3><br />
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<p><span class="STYLE3">We have recorded 5 open classes and uploaded to the web aiming at giving people a whole picture of synthetic biology. </span></p><br />
<p><span class="STYLE3">Now those open classes are watched by more than 3000 people, and have got more than 50 positive comments. </span></p><br />
<p class="STYLE3"><strong>Lecture 1: Introduction to synthetic biology.</strong> It focuses on the history and applications of this searching area.</p><br />
<p class="STYLE3"><strong>Lecture 2: Interesting stories about synthetic biology.</strong> We want to let more people have an interest in scientific research.</p><br />
<p class="STYLE3"><strong>Lecture 3: About central dogma.</strong> This is the most important and most beautiful theory in synthetic biology. We hope more people can know it.</p><br />
<p class="STYLE3"><strong>Lecture 4: About BioBrick and the basic idea in synthetic biology.</strong> Because BioBrick is the essence idea of synthetic biology.</p><br />
<p class="STYLE3"><strong>Lecture 5: About the lab work.</strong> Because biology is a kind of science that based on experiment. It will also be much fun for people who don’t have a chance to visit laboratory.<br /><br />
</p><br />
<p></p><br />
<h3 class="STYLE2"><font face="Arial, Helvetica">The link to our Open Classes: </font></h3><br />
<p class="STYLE3">We provided the links to our Open Classes. </p><br />
<p class="STYLE4">Youtube (non-Chinese user):</p><br />
<p class="STYLE3">Lecture 1: Introduction to synthetic biology: <a href="http://www.youtube.com/watch?v=9eqMyoCe1R0&feature=plcp">http://www.youtube.com/watch?v=9eqMyoCe1R0&amp;feature=plcp</a></p><br />
<p><span class="STYLE3">The </span><font face="Arial, Helvetica">collection of the whole 5 open classes: <a href="http://www.youtube.com/channel/UCnHeffOAwxhXhJhUo55x-BA?feature=guide">http://www.youtube.com/channel/UCnHeffOAwxhXhJhUo55x-BA?feature=guide</a></font></p><br />
<p><font face="Arial, Helvetica"> <a href="http://www.youtube.com/watch?v=9eqMyoCe1R0&feature=plcp"><img src=" https://static.igem.org/mediawiki/igem.org/b/b3/Z1YB95AED-_UNG~B81-M5%40J~1.jpg" class="img_fl img_border" /></a></font></p><br />
<p><strong><font face="Arial, Helvetica">Youku (Chinese user):</font></strong></p><br />
<p><font face="Arial, Helvetica"><span class="STYLE3">Lecture 1: Introduction to synthetic biology: <a href="http://v.youku.com/v_show/id_XNDUzOTYzMzU2.html"> http://v.youku.com/v_show/id_XNDUzOTYzMzU2.html</a></span></font></p><br />
<p><span class="STYLE3">The </span><font face="Arial, Helvetica">collection of the whole 5 open classes: <a href="http://i.youku.com/u/UNTI1NTM3ODg4">http://i.youku.com/u/UNTI1NTM3ODg4</a> </font> </p><br />
<p><font face="Arial, Helvetica"><a href="http://v.youku.com/v_show/id_XNDUzOTYzMzU2.html"> <img src=" https://static.igem.org/mediawiki/igem.org/c/c2/You.jpg" class="img_fl img_border" /></a></font><br><br />
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<h3 class="STYLE2"><font face="Arial, Helvetica">Comments from Audiences:</font></h3><br />
<font face="Arial, Helvetica"><br />
</font><br />
<p><font face="Arial, Helvetica">Our open classes have let thousands of Chinese who knew nothing about synthetic biology have a concept about it, and they are very well reviewed</font></p><br />
<p><font face="Arial, Helvetica">This was the record only two days since we had uploaded:</font></p><br />
<p><img src=" https://static.igem.org/mediawiki/igem.org/1/18/6.jpg" class="img_fl img_border" /></p><br />
<p><font face="Arial, Helvetica">There are the comments from Chinese audiences:</font></p><br />
<p><img src=" https://static.igem.org/mediawiki/igem.org/c/cd/0.jpg" class="img_fl img_border" /></p><br />
<p><span class="STYLE6"><font face="Arial, Helvetica">Translation</font></span><span class="STYLE5"><font face="Arial, Helvetica">: This lecture is very interesting and very easy to understand.</font></span></p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/a/ae/10.jpg" class="img_fl img_border" /></p><br />
<p><span class="STYLE6"><font face="Arial, Helvetica">Translation</font></span><span class="STYLE5"><font face="Arial, Helvetica">: Although I am not in this field, after watching this short lecture, I really have a brief view of the synthetic biology. SynBio really plays an important role in modern society. I am excited to see that there are so many awesome students from the SUSTC. The more Chinese students be like them, the better the future of China will be. Good job!!!.</font></span></p><br />
<p><img src=" https://static.igem.org/mediawiki/igem.org/0/03/21.jpg" class="img_fl img_border" /></p><br />
<p><span class="STYLE6"><font face="Arial, Helvetica">Translation</font></span><span class="STYLE5"><font face="Arial, Helvetica">: Great presentation, I will always remember your words about synthetic biology and its future. It will be better if you can pay more attention to some details like the resolution of your slides. West China School of Medicine.S.U, chenfan. </font></span></p><br />
<p><img src=" https://static.igem.org/mediawiki/igem.org/0/03/5.jpg" class="img_fl img_border" /></p><br />
<p><span class="STYLE6"><font face="Arial, Helvetica">Translation</font></span><span class="STYLE5"><font face="Arial, Helvetica">: The frame is clear, and the explanation is clear. But you are a little serious, it will be better if you are more humorous.</font></span></p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro
Team:SUSTC-Shenzhen-B/hp.intro
2012-10-26T21:28:06Z
<p>Water: </p>
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.pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
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<h1 class="STYLE7"><p>Human Practice Overview</p></h1><br />
<br />
<p class="STYLE7">In order to promote the awareness and education of synthetic biology in China, this summer, SUSTC IGEM team has done a remarkable job in motivating all kinds of people to study synthetic biology.</p><br />
<p class="STYLE9"><span class="STYLE12"><b>1.Open Classes.</b></span> <br><br />
<p>We have record 5 open classes and uploaded to the web aiming at giving people a whole picture of synthetic biology. These 5 classes cover many fields.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;a. Introduction to synthetic biology. It focuses on the history and applications of this searching area.<br />
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;b. Interesting stories about synthetic biology. We want to let more people have an interest in scientific research.<br />
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;c. The third part is about central dogma. This is the most important and most beautiful theory in synthetic biology. We hope more people can know it.<br />
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;d. BioBrick is the essence idea of synthetic biology. <br />
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;e. The last part involves the lab work. Because biology is a kind of science that based on experiment. It will also be much fun for people who don’t have a chance to visit laboratory. </p><br />
<p class="STYLE9"><span class="STYLE12"><b>2.Article </b></span><br />
<br>2. Our team has written an article to help people distinguish synthetic biology from genetic engineering.</p><br />
<p class="STYLE9"><span class="STYLE12"><b>3. Speeches in high schools. </b></span><br>We have hold 3 speeches in high schools to motivate high school students to study synthetic biology and guide them to participate in IGEM High School Division. We think that the development of a new subject really needs creative teenagers and future scientists to push forward.</p><br />
<p class="STYLE9"><span class="STYLE12"><b>4. Posters.</b> </span><br>Besides, we have made 8 posters to let people who attend our lectures have a further understanding of our project and synthetic biology.<span class="STYLE7"><br><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/September
Team:SUSTC-Shenzhen-B/September
2012-10-26T21:27:32Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
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<h1>September</h1><br />
<h3><span class="STYLE10">Abstract</span></h3><br />
<p>We came back to school to continue our work in school. So we had to deal with some problems such as our own laboratory and our plan in next week.</p><br />
<br><br />
<h3><span class="STYLE10">Week 1</span></h3><br />
<h3><span class="STYLE10">9.02.2012-9.08.2012</span></h3><br />
<p>9.03.2012</p><br />
<p>Tonight, we had our first human practice. We gave a lecture on synthetic biology to the freshman in our school, and showed them the blueprint of this field. Then we did a presentation on what we are doing right now. They showed great interests in our project and would like to join us.</p><br />
<br><br />
<p>9.04.2012</p><br />
<p>We had a meeting to discuss our plan for September. Each group member was assigned a specific role: Zhang Mengshi, Liu Xianggeng and I will be responsible for experiments. Wu Zishan and Shen Liuxing will prepare the lecture and posters which will be used in our planned tours to a couple of high schools in Shenzhen. We are going to introduce synthetic biology and our project to them. Chen Yao and Qie Boyu will optimize our program and finish our web page.</p><br />
<br><br />
<p>9.05.2012</p><br />
<p>We paid a visit to SIAT to discuss the potential of using their lab for our experiments. Luckily, they agreed.</p><br />
<br><br />
<h3>Week 2</h3><br />
<h3>9.09.2012-9.15.2012</h3><br />
<p>9.10.2012</p><br />
<p>We had a meeting to check whether our plan goes well. However, because of the new semester, it is a little bit harder to keep up with our schedule. I decided to put the deadline to 9.15.</p><br />
<br><br />
<p>9.15.2012</p><br />
<p>We had a meeting to discuss our plan for September. Each group member was assigned a specific role: Zhang Mengshi, Liu Xianggeng and I will be responsible for experiments. Wu Zishan and Shen Liuxing will prepare the lecture and posters which will be used in our planned tours to a couple of high schools in Shenzhen. We are going to introduce synthetic biology and our project to them. Chen Yao and Qie Boyu will optimize our program and finish our web page.</p><br />
<br><br />
<h3>Week 3</h3><br />
<h3>9.16.2012-9.22.2012</h3><br />
<p>9.17.2012</p><br />
<p>After all the material we bought arrived, we started our lab work again in our school. It’s a hard work to develop our own laboratory. But we had fun at the same time.</p><br />
<p>We did a digest and amplify GFP & RFP with PCR.<br />
</p><br />
<br><br />
<p>9.18.2012-9.20.2012</p><br />
<p>We visited 3 high schools and gave presentations to their students on synthetic biology and our IGEM projects. There are many students being interested in biology and some of them would like to join this competition. We think our major goal has been achieved.<br />
We also did a ligation to connect GFP, RFP and the plasmid together. Then we did a transformation.<br />
</p><br />
<br><br />
<p>9.21.2012</p><br />
<p>Today’s morning, we saw that control group almost exist only few of bacterial colony and the experimental group grow lots of colonies. I think it is a positive suggest. So we did a colony PCR to test whether the ligation is successful. We picked up 10 colonies to test and three of them is positive ligated by three parts and all the colonies are positive ligated by two parts.</p><br />
<br><br />
<p>9.22.2012</p><br />
<p>We picked up those colonies which are positive. Through the bacterium culture in shake-flask more than 12 hours, we can do plasmid miniprep.</p><br />
<p>We also started to record our open classes. </p><br />
<p>Because the deadline of wiki is coming so almost every team members are busy in writing their parts.</p><br />
<br><br />
<h3>Week 4</h3><br />
<h3>9.23.2012-9.27.2012</h3><br />
<p>This is the last week to edit our wiki, so does our project. So our team members spared no efforts to accomplish the work. Thankfully, we finished our work before the deadline. </p><br />
<br> <br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/august
Team:SUSTC-Shenzhen-B/august
2012-10-26T21:27:09Z
<p>Water: </p>
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<br />
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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
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<br />
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<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
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var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
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$('#menu-fontsize').remove();<br />
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$ul.css('width',emWidth);<br />
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$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
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var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
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<br />
});<br />
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// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
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jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
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img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
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canvas.style.width = w+"px";<br />
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ctx.drawImage(this, 0, 0);<br />
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var data = ctx.getImageData(0, 0, w, h).data,<br />
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png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<br />
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<br />
<article><br />
<h1>August</h1><br />
<h3><span class="STYLE10">Abstract</span></h3><br />
<p>The first week one of our group should choose more than one hundred terminator sequences to be tested. The other group is to learn how to do experiment and design the lab protocol.</p><br />
<h3><span class="STYLE10">Week 1</span></h3><br />
<h3><span class="STYLE10">8.01.2012-8.04.2012</span></h3><br />
<p>8.01.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We learned how to distinguish rho-dependent and rho-independent terminators.</p><br />
<p>2. We tried to design different terminators from one template so we can simplify the final analysis graph and we can find the relationship between energy and efficiency more clearly.</p><br />
<p>Lab Group:</p><br />
<p>1. We tried to make clear of what we need to do if we have got the sequence of the terminator.</p><br />
<p>2. After we understood the procedure of our experiment, we started to learn how to use these equipments. It was the first time we can touch these precise equipments so we felt excited.<p><br />
<br><br />
<p>8.02.2012</p><br />
<p>We contacted several companies to compare their DNA sequence prices and decided to make a purchase from Ying Jun with whom we came to an agreement of ¥1.5/bp. We have never negotiated with any company before. It was a really fantastic experience.</p><br />
<p>Here are our criteria for terminator selection:</p><br />
<p>1. It is not too long;</p><br />
<p>2. It does not have anti-loop;</p><br />
<p>3. It is a forward sequence.</p><br />
<p>We spared no pains in this effort-checking reference, searching the web, using web software, and getting advices from our advisor. Almost everyone stayed up late today.</p><br />
<p>Finally we found 12 terminators meet our requirements and made a synthesis of those sequences at the end.</p><br />
<br><br />
<p>8.03.2012</p><br />
<p>We add the restriction enzyme binding site sequences to terminators to generate a list of sequences, then submit the list to the manufacturer by the deadline.</p><br />
<p>Today is Friday, so we decided to take a break. We saw a movie together.</p><br />
<br><br />
<p>8.04.2012</p><br />
<p>Today is Saturday, our team went to the beach together to enjoy the summer holiday.</p><br />
<br />
<br />
<div class="clear"></div><br />
<div class="separator line"></div><br />
<br />
<article class="row"><br />
<div class="one_fourth"><br />
<span class="dropcap4">1</span><br />
<h4>week 1</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">Read More</a><br />
</div><br />
<div class="one_fourth"><br />
<span class="dropcap4">2</span><br />
<h4>week 2</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 2/8">Read More</a><br />
</div><br />
<div class="one_fourth"><br />
<span class="dropcap4">3</span><br />
<h4>week 3</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 3/8">Read More</a><br />
</div><br />
<div class="one_fourth"><br />
<span class="dropcap4">4</span><br />
<h4>week 4</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 4/8">Read More</a><br />
</div><br />
<div class="one_fourth"><br />
<span class="dropcap4">5</span><br />
<h4>week 5</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 5/8">Read More</a><br />
</div><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/july
Team:SUSTC-Shenzhen-B/july
2012-10-26T21:26:42Z
<p>Water: </p>
<hr />
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5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li .smalldate{display:block;font-size:11px;font-style:italic;overflow:hidden}#footercontainer{background:#3f4432;-webkit-border-bottom-left-radius:7px;-webkit-border-bottom-right-radius:7px;-moz-border-radius-bottomleft:7px;-moz-border-radius-bottomright:7px;border-bottom-left-radius:7px;border-bottom-right-radius:7px}#footer{padding:25px 0 25px 0;color:#bbb9a1;font-weight:400;font-family:'Open Sans',sans-serif;font-size:13px}#footer a,#footer a:visited{color:#bbb9a1}<br />
<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
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<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
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</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
</nav><!-- nav --><br />
<br />
<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
<div id="logo" class="frontpage"><a href="#"><img src="https://static.igem.org/mediawiki/2012/3/3a/Tihuan.JPG" alt=""></a></div><br />
<div class="clear"></div><br />
</header><br />
</div><br />
<!-- END HEADER --><br />
<br />
<!-- MAIN CONTENT --><br />
<div id="outermain"><br />
<div id="maincontent"><br />
<section id="mainthecontent"><br />
<br />
<article><br />
<h1><p>July</p></h1><br />
<h3 class="STYLE10">Abstract</h3><br />
<p>This is the first week that we started our team work. We hold a meeting to allocate everyone’s work. We are divided into 3 groups. The first group is focused on reading paper to learn what is terminator. The second group is responsible for software. The third group should build a web page for our software. None of us is specialist for computer language or biology, so our work is hard.</p><br />
<br><br />
<h3 class="STYLE10">Week 1</h3><br />
<h3 class="STYLE10">7.15.2012-7.21.2012</h3><br />
<p class="STYLE11">7.15.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We read some paper, focused on studying two of them.</p><br />
<p>2. We tried to find paper on terminator efficiency, but we didn’t make much progress.</p><br />
<p>Software Group:</p><br />
<p>We wrote a small program with C++. It can return LH and nt when input stem loop and T tail sequence. If delta G is known, it will give d score automatically.</p><br />
<p>Web Group:</p><br />
<p>1. We prepared the first draft wiki and decided to improve it later when we have more experience on web design.</p><br />
<p>2. We borrowed a couple of books about C# and web design from the library.</p><br />
<br><br />
<p class="STYLE11">7.16.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We created a table of terminator efficiency including fields of name, description, sequence, stem, loop, tail and efficiency.</p><br />
<p>2. We searched web of knowledge and google scholar for transcription terminator paper.</p><br />
<p>3. We emailed authors of those paper to clear out confution and ask questions about T.E.</p><br />
<p>Software Group:</p><br />
<p>We learned some basic knowledge of C#, wrote a program to calculate d score and nt. But users have to type in the free energy.<br />
Web group:</p><br />
<p>1. We learned how to build a simple web page.</p><br />
<p>2. We spent the whole night in solving a simple but strange problem. Fortunately we conquered it.</p><br />
<br><br />
<p class="STYLE11">7.17.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We found some information on terminator and some specific terminators, learned the progress of writing our RNA fold program.</p><br />
<p>2. We searched more terminator efficiency data, read some materials about how to write a paper, what to be prepared and concerned.</p><br />
<p>Software Group:</p><br />
<p>We continued our C# programming study, improved our program to calculate d score and nt.</p><br />
<p>Web Group:</p><br />
<p>1. We learned how to use dreamweaver to design a simple web page.</p><br />
<p>2. We learned how to build a database.</p><br />
<span class="STYLE11"><br><br />
</span><br />
<p class="STYLE11">7.18.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We visited some terminator structure and mechanism websites.</p><br />
<p>2. We read a paper about terminator; contacted several researchers to get information. We tried to imitate existing algorithm to determine terminator’s secondary structure. The first version of the program has been developed and we are going to test it.</p><br />
<p>3. We added more records to the TE database.</p><br />
<p>Software Group:</p><br />
<p>We improved the input validation program and made the class-”d_score_calculator” more usable. We read the manual of mfold and calculated the free energy of 1-loop by ourselves. But the free energy of 2-loop is still a problem.</p><br />
<p>Web Group:</p><br />
<p>1. We designed our team logo; learned how to build a server for our website.</p><br />
<p>2. We obtained and installed all the softwares we are going to use.</p><br />
<p>3. We were still trying to design a user-friendly web page.</p><br />
<br><br />
<p class="STYLE11">7.19.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We read some paper on terminator efficiency and found lots of models about the mechanism of terminator.</p><br />
<p>2. We learned some knowledge about double terminator which we didn’t know before. He also built a database about terminator efficiency which is a big contribution to our group.</p><br />
<p>Software Group:</p><br />
<p>1. We converted Liu Xing’s C++ program into C#, added the input validation function. Then we created an interface for the hairpin score calculation.</p><br />
<p>2. We calculated the free energy of 2-loop containing a bulge loop and a hairpin loop.</p><br />
<br />
<p>Web Group:</p><br />
<p>1. We learned how to use WPF to build a windows program and updated our server.</p><br />
<p>2. We built up the basic structure of our web page.</p><br />
<br><br />
<p class="STYLE11">7.20.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We read some paper on two RNA polymerase termination models, which has a higher view of terminator mechanism; and paper on termination mechanism models.</p><br />
<p>2. We updated and optimized TE database.</p><br />
<p>3. We improved the stem loop determination algorithm and modularized program.</p><br />
<p>Software Group:</p><br />
<p>We programmed the algorithm to make sure the structure of the terminator is clear.</p><br />
<p>Web Group:</p><br />
<p>1. We continued building a windows program.</p><br />
<p>2. We continued improving our web page.</p><br />
<br><br />
<p class="STYLE11">7.21.2012</p><br />
<p>Paper Group:</p><br />
<p>1. We did a second view of all paper and “why we do a research of terminator efficiency”.</p><br />
<p>2. We updated our database again.</p><br />
<p>Software Group: </p><br />
<p>1. We continued optimizing our algorithm. </p><br />
<p>2. We read some paper and material. We can create the algorithm based on one pair of “closing base pair”.</p><br />
<p>Web Group:</p><br />
<p>1. We finished the UI of our windows application program.</p><br />
<p>2. We continued improving our web page.</p><br />
<br />
<div class="clear"></div><br />
<div class="separator line"></div><br />
<br />
<article class="row"><br />
<div class="one_fourth"><br />
<span class="dropcap4">1</span><br />
<h4>week 1</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">Read More</a><br />
</div><br />
<div class="one_fourth"><br />
<span class="dropcap4">2</span><br />
<h4>week 2</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 2">Read More</a><br />
</div><br />
<div class="one_fourth"><br />
<span class="dropcap4">3</span><br />
<h4>week 3</h4><br />
<p>Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.</p><br />
<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 3">Read More</a><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june
Team:SUSTC-Shenzhen-B/before.june
2012-10-26T21:26:10Z
<p>Water: </p>
<hr />
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<br />
/* prettyPhoto.css */<br />
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no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery 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no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px 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.pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/before.june&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/before.june&amp;action=history">History</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
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<h1><p>Before June</p></h1><br />
<p>We hold a journal club to give everybody a chance to present what they have learnt from paper. According to this activity, all of us have learnt a lot knowledge about synthetic biology and known the frontiers of science. </p><br />
<br><br />
<h3 class="STYLE9"><span class="STYLE10">Brainstorming</span></h3><br />
<p>Before our team decides to make a transcription terminator efficiency calculator which is our project for igem, we held a brainstorm to find an idea which can become our project and will work, which everyone propose many impressive ideas. Then, we are going to see what are proposed. </p><br />
<br />
<p>First, a software called “Potbear” design by Xianggeng Liu, Yao Chen, and Zishan Woo. It is an all-in-one software, which can deal with all things about genes that you want to create. Users can choose promoter, ribosome binding site, terminator and other functional biobricks in themselves’ ways that they want. In some way, it is a friendly and strong database.</p><br />
<br />
<p>Secondly, Liuxing Shen and Mengshi Zhang want to design and create a new organelle which can translate electric signal to RNA and memorize information by using reverse transcriptase. It can produce products that other kind of cells can do by input different electric signal, like Taxol. Besides, the organelle is expected to live in cancer cells or E.coli cells.</p><br />
<br />
<p>Finally, the idea which we make true designed by Boyu Qie and Shui Jing is TTEC, in other words, transcription terminator efficiency calculator. Users can use it online or download by PC. The main function of TTEC is that calculate the terminators’ user offered efficiency. Besides, it also provides a terminator database for users to select terminator they need.</p><br />
<br />
<p>After thinking and talking, we decide to make true TTEC, and we finish it successfully. </p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement
Team:SUSTC-Shenzhen-B/Acknowledgement
2012-10-26T21:25:44Z
<p>Water: </p>
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.widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li 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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook 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.pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions
Team:SUSTC-Shenzhen-B/Contributions
2012-10-26T21:24:46Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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.pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
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<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/Contributions&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/Contributions&amp;action=history">History</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
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<article><br />
<h1><p>Contributions</p></h1><br />
<h3 class="STYLE10">Team Members</h3><br />
<br><br />
<h3>Shui Jing</h3><br />
<p>1. Designed the first version of our TTEC webpage.</p><br />
<p>2. Participated and supervised the lab work and experimental schedule.</p><br />
<p>3. Was responsible for building our wiki pages.</p><br />
<p>4. Was corresponding author of article technical standard.</p><br />
<p>5. Arranged team affairs.</p><br />
<br><br />
<h3>Xiangeng Liu</h3><br />
<p>1. Updated terminator efficiency database.</p><br />
<p>2. Was one of the two chief experiment group members.</p><br />
<p>3. Wrote the part of “Safety”.</p><br />
<br><br />
<h3>Zishan Wu</h3><br />
<p> 1. Wrote a program to calculate the free energy change of RNA folding, which was part of d'Aubenton Carafa Scoring System.</p> <p> 2. Wrote some small programs to help lab group, such as adding restriction sites to DNA sequences.</p><br />
<p> 3. Helped to choose terminator sequence for lab work. </p><br />
<p> 4. Combined the programs of two scoring systems together and analyzed the data from Biofab Terminator Library to make simulation formula of d score and termination efficiency and used our own experiment data to validate the prediction of TTEC.</p><br />
<br><br />
<h3>Mengshi Zhang</h3><br />
<p>1. Wrote protocol notebook and lab results.</p><br />
<p>2. Was one of the two chief experiment group members.</p><br />
<p>3. Designed the team T-shirt.</p><br />
<br />
<br><br />
<h3>Liuxing Shen</h3><br />
<p>1. Provided suggestions of the programming from beginning to the end.</p><br />
<p>2. Read paper to shed light on the work and gave suggestions of all kind.</p><br />
<p>3. Wrote an article about synthetic biology and designed the boards.</p><br />
<p>4. Went to high school to get the video of the event.</p><br />
<br><br />
<h3>Yao Chen</h3><br />
<p>1.Wrote the first program for TTEC.</p><br />
<p>2.Wrote several simple programs for further application.</p><br />
<p>3.debugged for the programme. </p><br />
<br><br />
<h3>Boyu Qie</h3><br />
<p>1. Built and set our server of Website.</p><br />
<p>2. designed our user interface of PC tool and online tool, and wrote them. </p><br />
<p>3. wrote parts of the wiki.</p><br />
<p>4. went to other school to visit other teams to get their advice about our project.</p><br />
<br />
<br />
<br />
<h3 class="STYLE10">Advisers</h3><br />
<p class="STYLE10">&nbsp;</p><br />
<br />
<h3>Jiankui He</h3><br />
<p>Dr. He is the instructor of SUSTC_Shenzhen_B. Dr.He mentored the entire project from organizing journal club, designing the project. Dr.He met team members regularly once a week. </p><br />
<br><br />
<h3>Rui Zhao</h3><br />
<p>1. Recommend us good books on C/C++. </p><br />
<p>2. Give us advisce about programming when we feel puzzle.</p><br />
<br><br />
<h3>Yi Deng</h3><br />
<p>1. Give us advice on experimental planning.</p><br />
<br><br />
<h3>YinLan Zhao</h3><br />
<p>1. Give us advice on experiments.</p><br />
<p>2. Give us some suggestions to solve the experimental problems.</p><br />
<br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/photos
Team:SUSTC-Shenzhen-B/photos
2012-10-26T21:23:08Z
<p>Water: </p>
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.pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
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<h1><p>Photo Gallery - Our Memory</p> </h1><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers
Team:SUSTC-Shenzhen-B/advisers
2012-10-26T21:21:47Z
<p>Water: </p>
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-100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
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<h1><p>Advisers</p></h1> <br />
<br />
<h3>Jiankui He</h3><br />
<img src=" https://static.igem.org/mediawiki/2012/2/20/He.JPG" class="img_fl img_border alignright"/><br />
<p>Dr. Jiankui He is an associate professor at SUSTC. Dr. He got his PhD degree in Physics at Rice University in Houston Texas in 2010 and did his postdoc at Stanford University.</p> <br />
<p>Dr. He's lab employs interdisciplinary methods in physics, chemistry, and statistics to study biological systems at both cellular and genetic level. Current research focuses on genomic, human genetic diseases, immunology, system biology and synthetic biology.</p> <br />
<br><br />
<br />
<h3>Yi Deng</h3> <br />
<img src="https://static.igem.org/mediawiki/2012/1/1e/Yideng.PNG" class="img_fl img_border alignright" /> <br />
<p>Dr. Deng Yi was graduated from Department of Biology at Shandong University with a bachelar’s s degree in 1996 and a master’s degree in 1999. In 2002, she graduated magna cum laude with a Ph. D. degree from Ruhr-University Bochum, Germany. From 2003 to 2008, she did the postdoctoral training at Naitonal Institutes of Health (USA). After moving to Hong kong with her family she worked in the Hong Kong University of Science and Technology and the Chinese University of Hong Kong, and in 2012 she joined South University of Science and Technology of China as an Associate Professor. Dr. Deng has a broad interest in the field of cell biology and her work has addressed fundamental questions about membrane trafficking and intracelluar proterin transport as well as cell cycle regulation and ageing. Currently her research interestes are mainly focused on two aspects: 1. the regualtion of proterin machinaries that control cell adhesion and mirgation; 2. the molecular mechanisms of human islet amyloid polypeptide-induced pancreatic beta cell damage. </p><br />
<h3>Rui Zhao</h3> <br />
<img src="https://static.igem.org/mediawiki/2012/1/13/Rui.JPG" class="img_fl img_border alignright" /><br />
<br />
<p>Mr. Rui Zhao got his bachelor degree and master degree in Tsinghua University, majored in applied mathematics. His research interests mainly focus on computational and bioinformatics. He joined in the South University of Science and Technology of China last year then participated this IGEM program.</p><br />
<br><br><br><br><br />
<h3>Yinlan Zhao</h3> <br />
<p>I was graduated from Wuhan Institute of technology, majored in bioengineering in 2005. In 2012, I got my Ph.D degree from Medical School of Wuhan University. I was interested in innate immunity and molecular biology of virus.</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/members
Team:SUSTC-Shenzhen-B/members
2012-10-26T21:21:25Z
<p>Water: </p>
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no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
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<h1><p>Team Members</p></h1><br />
<h3>Shui Jing</h3><br />
<img src="https://static.igem.org/mediawiki/2012/c/ce/Js.jpg" class="img_fl img_border alignright" /><br />
<p>I’m Shui Jing, a sophomore and still don’t have major. All the wonders in physics and biology attract me so much that I really enjoy the process of learning.</p><br />
<p>In my spare time, I am involved in all kinds of sports, such as basketball, badminton, table tennis swimming and many more. <br />
As a team leader, my major task in this project is arranging the team affairs, I am also responsible for our wiki. I think I have learned a lot from this special experience. learning how to cooperate with team members from different background and different characters and how to shoulder the responsibilities I have to face are not as easy as what you say. The more difficulties I overcome, the more mature I will be.</p><br />
<p>I hope everyone can have fun in IGEM and enjoy the process.</p><br />
<p>E-mail:betty.jingshui@gmail.com</p> <br />
<br><br />
<h3>ZiShan Wu</h3><br />
<img src="https://static.igem.org/mediawiki/2012/2/26/Shan.JPG" class="img_fl img_border alignright" /><br />
<br />
<p>I’m Zishan Wu. My friends call me big potato because I am like the cartoon character in ‘Old Master’. I always think I am lucky because I met many wonderful things. So, joining in iGEM team is my luckiness ,too.Our team divide into lab group and theory group. I chose the theory group because I liked writing programs and reading some intersting papers. I really enjoyed the process where I learned many things and got closer to our goal step by step.</p><br />
<br><br><br><br><br><br />
<h3>MengShi Zhang</h3><br />
<img src="https://static.igem.org/mediawiki/2012/8/83/James-sustc.JPG" class="img_fl img_border alignright" /><br />
<p>Email: mengshi0928@gmail.com</p><br />
<p>Hobbies: Reading ,Sleeping ,designing & handiwork</p><br />
<p>Education: 2011.3-2012.3 General education in SUSTC,China </p><br />
<p>Contribution to our team: Be a member of experiment group and design the T-shirt on the leisure time. </p><br />
<p>Statement : Beginning with no professional knowledge and skills, I am eager to learn everything. The process of the whole experiment is a procedure of learning and summarizing. There is no time to delay. Learn from mistakes. Be responsible for your data and results. <br />
Motto: Young and challenge yourself. Nothing is impossible.</p><br />
<h3>Yao Chen</h3><br />
<img src=" https://static.igem.org/mediawiki/2012/8/80/Cyao.JPG" class="img_fl img_border alignright" /><br />
<p>I am Chen Yao. I like writing and reading, and I love science. I like both failure and success. Failure makes me mature, and success after many times of failure makes failure meaningful. I attended iGEM because I really enjoyed doing something special. In our team, I am one of the "programmers" and I wrote the program hairpinscore in C#.</p><br />
<h3>BoYu Qie</h3><br />
<img src="https://static.igem.org/mediawiki/2012/9/94/Qie.JPG" class="img_fl img_border alignright" /><br />
<p>You can call me Qie Boyu, which is my name, or Qie Ye, which means grandfather Qie made by my friends to show their honour(ahahaha~). I'm from Chengdu, the best city in the world. In order to find some meaningful project to do in the summer vacation, I take part in our iGEM team though I nearly didn't known everything out of biology tought in high school. Doing the project with my friends is very cool, I totally fall in love with the process of it. I'm loving it !</p><br />
<h3>Xianggeng Liu</h3><br />
<img src="https://static.igem.org/mediawiki/2012/7/72/Liu.jpg" class="img_fl img_border alignright" /><br />
<p>I am Xianggeng Liu, a undergraduates from SUSTC, and will major in chemistry. I am responsible for database, lab and wiki. My hobbies are chemistry, history and football. iGEM gives me a chance to understand synthetic biology and do some research, which makes me enjoy science.</p><br />
<h3>Liuxing Shen</h3><br />
<img src="https://static.igem.org/mediawiki/2012/e/e0/Shen.JPG" class="img_fl img_border alignright" /><br />
<p>Hi, I'm liuxingshen and a member of terminator team. Instead of wetlab, I focused on lecture and computer stuffs. This was my first time to involve in a big team projects and I found so much things new and fascinating. During this summer ,I have learned how to trust teammates and how to distribute the tasks to them. Sometimes, I was tired and desperate but, after all, it was a thrill to experience something like this!</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC
Team:SUSTC-Shenzhen-B/RFC
2012-10-26T21:20:48Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
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<h1>RFC Technical Standard </h1><br />
<br><br />
<h3>Brief Introduction:</h3><br />
<p>Technical standards framework is formed to reduce the complexity and cost of producing synthetic living organisms by leveraging time-honored engineering principles of abstraction and standardization. This blend of technology and biology makes it easier for scientists and engineers to work together to develop practical solutions for serious problems facing mankind.</p><br />
<p>In order to help other people to do research on this field, our efficiency measurement protocol has been submitted to BioBrick foundation as a technical standard(BBF RFC 90).</p><br />
<p>For more information about this paper, please go to <a href="http://dspace.mit.edu/handle/1721.1/73911">BBF RFC 90</a></p> <br />
<br><br><br />
<p><h3>BBF RFC 90:The Measurement of rho-independent Transcription Terminator<br />
Efficiency.</h3></p><br />
<p>Jiankui He, Shui Jing, Mengshi Zhang, Xianggeng Liu, Yao Chen, Boyu Qie, Liuxing<br />
Shen Zishan Wu</p><br />
<br />
<p>26 September 2012</p><br />
<br />
<p><h4><b>1. Purpose</b></h4></p><br />
<p>The purpose of this RFC is to provide standard methodology for the measurement<br />
of the absolute strength of terminators in bacteria. Because we have characterized<br />
the performance of terminator in E.coli and used a simple equation model, it can be<br />
expressed in PoPS.</p><br />
<br />
<p><h4><b>2. Relation to other BBF RFCs</b></h4></p><br />
<p>This paper doesn’t relate to any other BBF RFC.</p><br />
<br />
<p><b><h4>3. Copyright Notice</b></h4></p><br />
<p>Copyright (C) The BioBrick foundation(2012).All Right Reserved.</p><br />
<br />
<p><b><h4>4. Standard terminator efficiency measurement kit for use in E.coli.</b></h4></p><br />
<p>The terminator efficiency measurement kit MUST include promoter, ribosome<br />
binding sites, reporter gene and vector backbone. Standard transcription terminator<br />
efficiency measurement kit MUST comprise of promoter BBa_R0040, ribosome<br />
binding site BBa_B0030, reporter gene, RFP BBa_E1010, GFP BBa_E0040. It SHOULD<br />
comprise vector backbone pSB1A3, a high copy number plasmid carrying ampicillin<br />
resistance. This high copy number vector is chosen to facilitate growth of bacteria<br />
step in the experiment</p><br />
<br />
<p><b><h4>5. Design and construction of characterization devices.</b></h4></p><br />
<p>The fluorescence produced by the characterization devices are then measured using<br />
flow cytometry to calculate the termination efficiency of the terminators.</p><br />
<p>Experimental Group:</p><br />
<img src="https://static.igem.org/mediawiki/2012/7/7e/Experiment.JPG" class="img_fl img_border" /><br />
<br />
<p>(Inputs are measured by RFP expression and outputs are measured by GFP<br />
expression.)</p><br />
<br />
<p>Control Group:</p><br />
<img src="https://static.igem.org/mediawiki/2012/c/cf/Control.JPG" class="img_fl img_border" /><br />
<p>(calibrates RFP input to GFP output.)</p><br />
<br />
<p><b><h4>6. Experimental procedure</b></h4></p><br />
<p>6.1 Media:</p><br />
<br />
<p>Lysogeny Broth (LB) liquid and solid media.</p><br />
<br />
<p>6.2 Protocol:</p><br />
<br />
<p>a. Plasmid pSB1A3 is chosen to be the vector that ligate GPF and RFP fragments.<br />
To protect the structural integrity of the constructed plasmid, we need to<br />
mutate a restriction enzyme cutting site named Pst I to Afl II using PCR.<br />
Proper primer are designed for this purpose.</p><br />
<br />
<p>PtoA-F:5'-CCACCTGACGTCTAAGAAAC-3'</p><br />
<br />
<p>PtoA-R:5'-ATGATCATCGCCGGCGAATTCAGGC-3'</p><br />
<br />
<p>b. Check whether the mutation of restriction cutting site is successful.</p><br />
<br />
<p>c.Transform the plasmid to competent cell DH5αand incubate overnight.</p><br />
<br />
<p>d. Pick up colonies, plasmid isolation, and digest to choose the positive<br />
one.</p><br />
<br />
<p>e. Amplify GFP & RFP. Do an electrophoresis for verification the PCR is<br />
successful.</p><br />
<br />
<p>f.<br />
Use specific restriction enzymes to digest plasmid mutant-psb1a3,GFP<br />
and RFP to get sticky ends and purify the DNA fragment after the<br />
Electrophoresis.</p><br />
<br />
<p>Digestion of plasmid mutant-pSB1A3 with Afl II and Not I;</p><br />
<br />
<p>Digestion of PCR product GFP with Not I and Spe I;</p><br />
<br />
<p>Digestion of PCR product RFP with Afl II and Spe I;</p><br />
<br />
<p>Put the tubes in 37℃ environment for 4-8 hours.</p><br />
<br />
<p>g. Ligation is needed to connect these 3 fragments together.</p><br />
<br />
<p>h.Transform the plasmid to competent cell DH5αand incubate overnight.</p><br />
<br />
<p>i.Pick up colonies, plasmid isolation, and choose the positive one.</p><br />
<br />
<p>j. Use restriction enzymes Xba I and Pst I to digest the constructed vector.</p><br />
<br />
<br />
<p>k. Ligation is needed to terminators to be measured and the vector together.</p><br />
<br />
Transform the plasmid to competent cell and incubate overnight.</p><br />
<br />
<p>l. Transform the plasmid to competent cell DH5αand incubate overnight.</p><br />
<br />
<p>m. Test the fluorescent using the method of flow cytometry.</p><br />
<br />
<p><b><h4>7. Parts used to create the terminator characterization.</b></h4></p><br />
<img src="https://static.igem.org/mediawiki/2012/3/33/Part.JPG" class="img_fl img_border" /><br />
<p><b><h4>8. Calculate terminator efficiency</b></h4></p><br />
<p>TE=1-GE/GC</p><br />
<p>TE: Terminator efficiency</p><br />
<p>GE: Mean value of GFP from experimental group</p><br />
<p>GC: Mean value of GFP from control group</p><br />
<br />
<p>Under the ideal circumstances, the output of GFP and RFP should be almost the same and<br />
the value of TE should approach 0. If a strong terminator is placed into characterization<br />
experimental group plasmid, the only output should be RFP and the value of TE should<br />
approach 1.</p><br />
<br />
<p><b><h4>9. Author’s contact information</b></h4></p><br />
<p>Jiankui He:<br />
jiankuihe@gmail.com</p><br />
<p>Shui Jing:<br />
betty.jingshui@gmail.com</p><br />
<p>Mengshi Zhang: mengshi0928@gmail.com</p><br />
<p>Xianggeng Liu: liuxianggeng@gmail.com</p><br />
<p>Yao Chen:<br />
chen.yy@live.com</p><br />
<p>Boyu Qie:<br />
boyuqie@gmail.com</p><br />
<p>Liuxing Shen: shenliuxing@gmail.com</p><br />
<p>Zishan Wu:<br />
zishan.woo@gmail.com</p><br />
<br />
<p><b><h4>10. References</b></h4></p><br />
<p>[1] http://partsregistry.org/wiki/index.php/Part_Types:Measurement_Systems</p><br />
<p>[2] Haiyao Huang “Design and Characterization of Artificial Transcriptional<br />
Terminators”.</p><br />
<p>[3] Lars Velten, Nao” Units for Promoter Measurement in Mammalian Cells”</p><br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol
Team:SUSTC-Shenzhen-B/lab.sbol
2012-10-26T21:19:27Z
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.widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li .smalldate{display:block;font-size:11px;font-style:italic;overflow:hidden}#footercontainer{background:#3f4432;-webkit-border-bottom-left-radius:7px;-webkit-border-bottom-right-radius:7px;-moz-border-radius-bottomleft:7px;-moz-border-radius-bottomright:7px;border-bottom-left-radius:7px;border-bottom-right-radius:7px}#footer{padding:25px 0 25px 0;color:#bbb9a1;font-weight:400;font-family:'Open Sans',sans-serif;font-size:13px}#footer a,#footer a:visited{color:#bbb9a1}<br />
<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
</ul><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/lab.sbol&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/lab.sbol&amp;action=history">History</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
</nav><!-- nav --><br />
<br />
<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
<div id="logo" class="frontpage"><a href="#"><img src="https://static.igem.org/mediawiki/2012/3/3a/Tihuan.JPG" alt=""></a></div><br />
<div class="clear"></div><br />
</header><br />
</div><br />
<!-- END HEADER --><br />
<br />
<!-- MAIN CONTENT --><br />
<div id="outermain"><br />
<div id="maincontent"><br />
<section id="mainthecontent"><br />
<br />
<article><br />
<h1><p>SBOL Document</p></h1><br />
<br />
<p>Synthetic Biology Open Language (SBOL) is a data exchange standard for descriptions of genetic parts, devices, modules, and systems. SBOL is neutral with respect to programming languages and software encoding. By supporting SBOL for reading and writing synthetic biology designs, different software tools can directly communicate and store the same representation of these designs. This removes an impediment to sharing engineered systems and permits other researchers and commercial enterprises to start with an unambiguous representation of the design.</p><br />
<br />
<br><br />
<br />
<p>The plasmids we construct are stored in gb and SBOL format.</p><br />
<br />
<p>You can download it, HERE:</p><br />
<li><a href="https://static.igem.org/mediawiki/2012/f/fb/WeblabDocuments.zip">WeblabDocuments.zip</a></li><br />
<br />
<br><br />
<br />
<img src="https://static.igem.org/mediawiki/2012/5/57/Control_plasmid_new.JPG" alt="" class="img_fl img_border" /> <br />
<br />
<img src="https://static.igem.org/mediawiki/2012/1/11/Experiment_plasmid_new.JPG" alt="" class="img_fl img_border" /> <br />
<br />
<br> <br />
<br />
</article><br />
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<br />
</html><br />
<br />
<div id="templatemo_main"><br />
<div class="col col23"><br />
<font face="Arial, Helvetica"> <br />
<h2><p>SBOL Document</p></h2><br />
<br />
<p>Synthetic Biology Open Language (SBOL) is a data exchange standard for descriptions of genetic parts, devices, modules, and systems. SBOL is neutral with respect to programming languages and software encoding. By supporting SBOL for reading and writing synthetic biology designs, different software tools can directly communicate and store the same representation of these designs. This removes an impediment to sharing engineered systems and permits other researchers and commercial enterprises to start with an unambiguous representation of the design.</p><br />
<br />
<br><br />
<br />
<p>The plasmids we construct are stored in gb and SBOL format.</p><br />
<br />
<p>You can download it, HERE:</p><br />
<li><a href="https://static.igem.org/mediawiki/2012/f/fb/WeblabDocuments.zip">WeblabDocuments.zip</a></li><br />
<br />
<br><br />
<br />
<img src="https://static.igem.org/mediawiki/2012/5/57/Control_plasmid_new.JPG" alt="" class="img_fl img_border" /> <br />
<br />
<img src="https://static.igem.org/mediawiki/2012/1/11/Experiment_plasmid_new.JPG" alt="" class="img_fl img_border" /> <br />
<br />
<br />
<br />
<br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/parts
Team:SUSTC-Shenzhen-B/parts
2012-10-26T21:19:00Z
<p>Water: </p>
<hr />
<div><html><br />
<head><br />
<meta charset="utf-8" /><br />
<meta name="description" content="" /><br />
<meta name="author" content="tengattack" /><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>Title</title><br />
<br />
<style type="text/css"><br />
#globalWrapper {width: 100%; }<br />
#top-section {width: 100%; height:100%; border:none;}<br />
#p-logo {display:none;}<br />
#search-controls {display:none;}<br />
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.firstHeading {display:none;}<br />
#content { border:none !important; width:1024px !important; background: url('') !important;}<br />
#bodyContent {border:none; }<br />
#catlinks {display:none; }<br />
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font-weight: 600;<br />
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}<br />
@font-face {<br />
font-family: 'Open Sans';<br />
font-style: normal;<br />
font-weight: 400;<br />
src: local('Open Sans'), local('OpenSans'), url(http://themes.googleusercontent.com/static/fonts/opensans/v6/cJZKeOuBrn4kERxqtaUH3T8E0i7KZn-EPnyo3HZu7kw.woff) format('woff');<br />
}<br />
<br />
/* inner */<br />
.pagenavi{clear:both;padding:0}.pagenavi a,.pagenavi a:visited{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#727272}.pagenavi a:hover{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#b03121;text-decoration:none}.pagenavi .current{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#b03121}.pagenavi .pages{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px}#breadcrumb{text-align:right;margin-bottom:34px;clear:right}.post{margin-bottom:30px;padding-bottom:30px;clear:both;border-bottom:1px solid #dfddc7}.post img{padding:5px;background:#d2d0ba;width:590px;height:236px}.single{padding-bottom:20px}.postimg{margin-bottom:20px}.posttitle{margin:0 0 5px 0}.posttitle,.posttitle a{font-size:18px;color:#3f4432}.posttitle a:hover{text-decoration:none}.entry-text{overflow:hidden;padding-left:30px}.entry-text h2{padding-top:5px}.entry-content{margin:0;padding:12px 0 5px 0}.entry-date{float:left;text-align:center;font-size:18px;width:56px;height:48px;padding-top:8px;line-height:normal;-moz-border-radius:56px;-webkit-border-radius:56px;-khtml-border-radius:56px;border-radius:56px;background:#3f4432;color:#f1efda;display:block}.month{font-size:11px;display:block}.entry-utility{padding:20px 0 0;font-size:11px;font-style:italic}.single .entry-utility{padding:0 0 0}#comment h2{margin-bottom:25px;text-transform:uppercase;font-size:14px}.commentlist{list-style-type:none;padding:0;margin:0}.commentlist ol{list-style-type:none;padding:30px 0 0 90px;margin:0}.commentlist li{position:relative;padding:0 0 30px 0}.commentlist li li{position:relative;padding:0}.avatar{position:absolute;top:0;left:0}.fn{font-size:12px;font-style:normal}.tdate{padding-left:30px}.tdate,.reply{font-size:11px;color:#a6a6a6;font-style:italic}.comment-body{margin:0 0 0 90px;padding:20px;background:#f7f5e3}.comment-body p{margin-bottom:5px;margin-top:10px}.comment-body .more{padding:0 0}#commentform{margin-bottom:15px}#commentform label{display:block}#commentform .text-input{margin-bottom:8px;padding:8px 5px;vertical-align:middle}#commentform .textarea{margin-bottom:20px;padding:8px 5px;vertical-align:top;width:90%}.ts-gallery-img img{max-width:100%;padding:0;margin:0 auto}.ts-gallery-clear{clear:both;height:1px!important;line-height:1px!important;float:none!important}.ts-gallery ul{list-style-type:none;margin:0;padding:0;clear:both}.ts-gallery ul li.nomargin{margin-right:0}.ts-gallery-text{padding:3px 0;text-align:center}.ts-gallery-text h2{font-size:13px;color:#3f4432;margin-bottom:20px;font-weight:600;font-family:'Open Sans',sans-serif}.no-gallery-text{display:none}.ts-gallery-img{background:#d2d0ba;padding:5px;position:relative;line-height:normal}.ts-gallery-img:hover{background:#3f4432}.ts-gallery-img a.image{display:block;position:relative;overflow:hidden;line-height:normal}.ts-gallery-img a 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0;width:300px;float:left}.ts-gallery-col3 li.nomargin{margin-right:0}.ts-gallery-col3 h2{margin:0 0 5px 0!important}.ts-gallery-col3 .ts-gallery-img{width:290px;height:115px}.ts-gallery-col3 .ts-gallery-img img{width:290px;height:115px}.ts-gallery-col3 .ts-gallery-img a.image{width:290px;height:115px;display:block;position:relative}.ts-gallery-col3 .ts-gallery-img a .rollover{width:290px;height:115px}.ts-gallery-col3 .ts-gallery-text{padding:5px 0 0 0}form{margin:0;padding:0}fieldset{border:0}#contactform{margin:0 auto;position:relative}#contactform h4{text-transform:uppercase;margin-bottom:20px}#contactform label{display:block;width:100%;float:left;padding-bottom:5px}span.required{color:#888}span.error{color:red;text-align:left;font-size:11px;padding-bottom:15px;display:block}#contactform input.text-input{margin-bottom:15px;vertical-align:middle;width:60%;float:left;font-style:italic;padding:8px;color:#727272;font-size:11px}#contactform textarea{width:95%;float:left;font-style:italic;color:#727272;font-size:11px;font-family:Arial,Helvetica,sans-serif}#message{margin-left:0;font-weight:700;color:red}#message h2{}#message p{margin:6px 0}.note{color:#d45454}#contactform .button{cursor:pointer;margin-top:20px;clear:both;float:left}<br />
<br />
/* style */<br />
html,body{height:100%}body{font-family:Arial,Helvetica,sans-serif;font-size:12px;color:#727272;margin:0;padding:0;line-height:20px;background:#d6d4c0 url(/wiki/images/d/d7/Sustc-b1-pattern.png) repeat}*{margin:0;padding:0}:focus{outline:0}form{margin:0;padding:0}hr{border-width:0;height:1px;line-height:0;margin:30px 0;page-break-after:always;text-align:center;width:100%;clear:both;color:#dfddc7;background-color:#dfddc7}.clearfix:before,.clearfix:after{content:'\0020';display:block;overflow:hidden;visibility:hidden;width:0;height:0}.clearfix:after{clear:both}.clearfix{zoom:1}.clear,.clr{clear:both;display:block;overflow:hidden;visibility:hidden;width:0;height:0}h1,h2{margin-bottom:25px}h3,h4,h5,h6{margin-bottom:10px}h1{font-size:26px}h2{font-size:20px}h3{font-size:16px}h4{font-size:14px}h5{font-size:12px}h6{font-size:10px}h1,h2{font-weight:400;font-family:'Open Sans',sans-serif;color:#404631}h3,h4,h5,h6{font-weight:600;font-family:'Open 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0;padding:0 10px 0 50px;background-image:url(/wiki/images/3/3b/Sustc-b1-quote.png);background-repeat:no-repeat;background-position:0 0;clear:both;font-family:Georgia,Arial;font-style:italic;font-size:16px;line-height:22px}blockquote.left,blockquote.right{float:right;letter-spacing:0;margin-bottom:20px;margin-left:20px;margin-top:0;padding:0 20px 10px 60px;width:43%;background-position:0 0}blockquote.left{float:left;margin-left:0;margin-right:20px}blockquote p{margin-bottom:0;font-size:16px;line-height:20px}code{font-family:Verdana,Arial;letter-spacing:1px;margin:25px 0 25px 0;display:block;font-size:.9em;border-left:4px solid #cfcfcf;padding:15px 10px}#bodychild{width:1000px;margin:0 auto;padding:50px 0}#outercontainer{width:1000px}#outerheader{-webkit-border-top-left-radius:8px;-webkit-border-top-right-radius:8px;-moz-border-radius-topleft:8px;-moz-border-radius-topright:8px;border-top-left-radius:8px;border-top-right-radius:8px}#outerfooter{border-top:1px solid 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5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li 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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square 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<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
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menu.sfTimer=setTimeout(function(){<br />
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$$.hideSuperfishUl();<br />
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if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
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getMenu = function($menu){<br />
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sf.op = sf.o[menu.serial];<br />
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},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
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return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
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sf.o[s] = sf.op = o;<br />
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this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
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hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
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var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
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// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
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<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
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</ul><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
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<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/parts&amp;action=edit">Edit</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
</nav><!-- nav --><br />
<br />
<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
<div id="logo" class="frontpage"><a href="#"><img src="https://static.igem.org/mediawiki/2012/3/3a/Tihuan.JPG" alt=""></a></div><br />
<div class="clear"></div><br />
</header><br />
</div><br />
<!-- END HEADER --><br />
<br />
<!-- MAIN CONTENT --><br />
<div id="outermain"><br />
<div id="maincontent"><br />
<section id="mainthecontent"><br />
<br />
<article><br />
<h1>Parts</h1><br />
<br />
<br> <br />
<p>As our experiment terminators, we added 95 terminators into <a href="http://partsregistry.org/Main_Page"><strong>partsregistry</strong></a> as biobricks.</p><br />
<p>The names of these biobricks range from <strong>BBa_K827000</strong> to <strong>BBa_K827094</strong> which you can search for on <a href="http://partsregistry.org/Main_Page"><strong>partsregistry</strong></a>.</p><br />
<br><br />
<img src="https://static.igem.org/mediawiki/2012/1/1a/Biobricks_of_our_own.JPG" width="700" height="450"<br />
alt="" class="img_fl img_border" /> <br />
<br />
<br> <br />
<br />
</article><br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/safety
Team:SUSTC-Shenzhen-B/safety
2012-10-26T21:18:29Z
<p>Water: </p>
<hr />
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<meta name="author" content="tengattack" /><br />
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Sans',sans-serif;-webkit-border-top-left-radius:3px;-webkit-border-top-right-radius:3px;-moz-border-radius-topleft:3px;-moz-border-radius-topright:3px;border-top-left-radius:3px;border-top-right-radius:3px}.tab-content{padding:20px 0}ul.tabs li:hover{}ul.tabs li.active{}html ul.tabs li.active a{color:#b03121;background:#e1dfc9;-webkit-border-top-left-radius:3px;-webkit-border-top-right-radius:3px;-moz-border-radius-topleft:3px;-moz-border-radius-topright:3px;border-top-left-radius:3px;border-top-right-radius:3px}#tab-body{padding:0 20px;background:#e1dfc9;border:0;-webkit-border-radius:3px;-webkit-border-top-left-radius:0;-moz-border-radius:3px;-moz-border-radius-topleft:0;border-radius:3px;border-top-left-radius:0}#toggle{margin:0 0 20px 0}h2.trigger{padding:0 0;margin:0;font-size:13px;background:transparent;font-family:arial;color:#3f4432}h2.trigger span{text-decoration:none;display:block;background:url(/wiki/images/9/93/Sustc-b1-toggle.png) no-repeat 0 5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li .smalldate{display:block;font-size:11px;font-style:italic;overflow:hidden}#footercontainer{background:#3f4432;-webkit-border-bottom-left-radius:7px;-webkit-border-bottom-right-radius:7px;-moz-border-radius-bottomleft:7px;-moz-border-radius-bottomright:7px;border-bottom-left-radius:7px;border-bottom-right-radius:7px}#footer{padding:25px 0 25px 0;color:#bbb9a1;font-weight:400;font-family:'Open Sans',sans-serif;font-size:13px}#footer a,#footer a:visited{color:#bbb9a1}<br />
<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded 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</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
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menu.sfTimer=setTimeout(function(){<br />
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return this.each(function() {<br />
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var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
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sf.o[s] = sf.op = o;<br />
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this.toggleClass(sf.c.shadowClass+'-off');<br />
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sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
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hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
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var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
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sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
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}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
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var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
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emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
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emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
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canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
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for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
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eval(a.join(""));<br />
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document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
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img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
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<!-- HEADER --><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<br />
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</ul><!-- topnav --><br />
</nav><!-- nav --><br />
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<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
<div id="logo" class="frontpage"><a href="#"><img src="https://static.igem.org/mediawiki/2012/3/3a/Tihuan.JPG" alt=""></a></div><br />
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<!-- END HEADER --><br />
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<!-- MAIN CONTENT --><br />
<div id="outermain"><br />
<div id="maincontent"><br />
<section id="mainthecontent"><br />
<br />
<article><br />
<h1>Safety</h1><br />
<div class="cleaner h40"></div> <br />
<p>The lab part of our project is basic molecule biology and cell biology experiment. There are not many problems in safety. The common sides in biological lab safety are biological hazard, chemical damage and physical danger.</p><br />
<br />
<p>Firstly, about biological hazard, the risk doesn’t exist in our lab mainly. The genes we need are GFP, RFP and AMP. They are harmless no matter when transcription and translation. Besides, the E. coli we used has already undergone mutation in gene level, which means the E. coli we used can’t cause any disease. </p><br />
<br />
<p>Secondly, as for chemical damage, there are some processes we have to care. The common method to check up the result of enzyme digestion and link of DNA fragment is electrophoresis. In a important process of electrophoresis, DNA is dyed by ethidium bromide. Ethidium bromide is a kind of poison. It is bad for our people health, and may cause cancer. So we must take care of it.</p><br />
<br />
<p>Finally, in physical danger, the most dangerous being is ultraviolet in the whole lab process, because other bacteria or fungi are forbidden appearing in some lab processes. </p><br />
<br />
<p>Besides, about the key questions, we can show our answers to you.</p><br />
<br />
<p><p class="STYLE5">Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?</p><br />
<br />
<p><p class="STYLE7">Researcher safety: </p><p>In Augest, Our team members, Shui Jing, Xianggeng Liu, Mengshi Zhang and Boyu Qie went to BGI to do experiments. On Augest 18th, we run a DNA gel and use the UV light to visualize the gel results. However, because we never used UV light before and did not know much about the safty guidline of UV light, we did not put the protection glass in front us, therefore, we are exposed to UV light for a few minutes. Although it is a small accedient, but we realized that we are lack of lab experience and should follow the instruction strictly.<br />
</p><br />
<br />
<p><p class="STYLE5">Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</p><br />
<br />
<p>Our new BioBricks do not raise any foreseeable safety issues. The Biobrick parts we made are transcription terminators, which is not translated into protein. </p><br />
<br />
<p><p class="STYLE5">Is there a local biosafety group, committee, or review board at your institution?</p><br />
<p><p class="STYLE5">• If yes, what does your local biosafety group think about your project?</p><br />
<p><p class="STYLE5">• If no, which specific biosafety rules or guidelines do you have to consider in your country?</p><br />
<br />
<p>Our university (SUSTC) is a brand new university which is established in 2011. So far, we do not have a biosafety committee in our institute. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafty level 2, which enable us to perform bacterial (E.coli) experiment safely. Many professor and assistant from biology and chemistry department of SUSTC care our safety and have checked the laboratory we used and the processes we have done. And they think the condition we were working is safe and reliable</p><br />
<br />
<p><p class="STYLE5">Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</p><br />
<br />
<p><p class="STYLE7">Here is our suggestions to iGEM headquarter: </p><p>Since many igem team members are very young, some of them may take lab course before. They are not familar with the lab safty guidline. Therefore, wet lab team members should take at least general biology lab course before they perform igem lab work.<br />
</p><br />
<br />
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<h2>Pictures </h2><br />
<img src="https://static.igem.org/mediawiki/2012/7/7e/Safety1.JPG" class="img_fl img_border" /><br />
<img src="https://static.igem.org/mediawiki/2012/9/9e/Safety2.JPG" class="img_fl img_border" /><br />
<img src="https://static.igem.org/mediawiki/2012/9/9d/Safety3.JPG" class="img_fl img_border" /><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_results
Team:SUSTC-Shenzhen-B/lab results
2012-10-26T21:18:01Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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.pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
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<p>Result</p><br />
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</font><h3 class="STYLE2"><font face="Arial, Helvetica">1. Fluorescence microscope results</font></h3><br />
<p>Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.<p><br />
<img src=" https://static.igem.org/mediawiki/2012/e/ec/81.png" class="img_fl img_border" ><br />
<p><span class="STYLE3">(Figure 1 Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. ) </span></p><br />
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<h3 class="STYLE2"><font face="Arial, Helvetica">2.Flow cytometry results </font></h3><br />
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<p><span class="STYLE3">Figure2-a</span></p><br />
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<p><span class="STYLE3">Figure2-b</span></p><br />
<p>Flow cytometry results of GFP expression in experiment group Figure2-a and control group Figure2-b.The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58</p><br />
<h3 class="STYLE2"><font face="Arial, Helvetica">3.Agreement with theoretical prediction</font></h3><br />
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<p class="STYLE3">(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)</p><br />
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<p class="STYLE3">(Figure 4 Here listed the sequences of terminator whose efficiencies are measured in the lab.)</p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1
Team:SUSTC-Shenzhen-B/protocol1
2012-10-26T21:17:36Z
<p>Water: </p>
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.pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
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<article><br />
<h1><p>Lab Protocol</p></h1><br />
</font><br />
<font face="Arial, Helvetica"><br />
<p><a href="#Site-Directed_Mutagenesis">1. Site-Directed Mutagenesis</a></p><br />
<p><a href="#Restriction">2. Mutation Verification by Restriction Enzyme Digestion</a></p><br />
<p><a href="#Media">3. Media Preparation</a></p><br />
<p><a href="#Bacterial">4. Bacterial Transformation</a></p><br />
<p><a href="#Colony">5. Colony PCR for Verification</a></p><br />
<p><a href="#Culture">6. Cultivate the Bacteria</a></p><br />
<p><a href="#Plasmid">7. Plasmid DNA Isolation</a></p><br />
<p><a href="#Restriction_2">8. Mutation Verification by Restriction Enzyme Digestion </a></p><br />
<p><a href="#Amplify">9. Polymerase Chain Reaction and Electrophoresis</a></p><br />
<p><a href="#Electrophoresis">10. Double Restriction Enzyme Digestion and Electrophoresis </a></p><br />
<p><a href="#Ligation">11. Ligation</a></p><br />
<p><a href="#Bacterial_Transformation">12. Bacterial Transformation</a></p><br />
<p><a href="#bacterial_colony">13. Bacterial Colony PCR</a></p><br />
<p><a href="#Culture_the">14. Cultivate the Bacteria</a></p><br />
<p><a href="#Plasmid_DNA">15. Plasmid DNA Isolation</a></p><br />
<p><a href="#Restriction_Enzyme">16. Restriction Enzyme Digestion and Electrophoresis</a></p><br />
<p><a href="#Ligation1">17. Ligation</a></p><br />
<p><a href="#Bacteria">18. Bacteria Transformation</a></p><br />
<p><a href="#Cultivate">19. Cultivate the Bacteria</a></p><br />
<p><a href="#Flow">20. Flow Cytometer Analysis</a></p><br />
<p><a href="#Fluorescence">21. Fluorescence Microscope </a></p><br />
<br/><br />
<br />
<p><br />
<a name="Site-Directed_Mutagenesis" ></a></font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Site-Directed Mutagenesis</font></b></font></h3><br />
<font face="Arial, Helvetica"><br />
Plasmid pSB1A3 was chosen as a backbone vector for cloning. The Pst I site in pSB1A3 was mutated to Afl II site to facilitate following cloning processes. Proper primers were designed and PCR-based site-directed mutageneis were carried out to generate this mutation as descbibed below. <br/><br />
<br />
<!--<p>要到很后面才能找到他的结束--><br />
<strong>Method:</strong><br/><br />
<br />
1. Set up PCR assay tubes as described below:<br />
<br/><br />
Total: 25 μl<br /><br />
+ 0.25 μl of Ex Taq polymerase <br /><br />
+ 2.5 μl of 10× Taq reaction buffer<br /><br />
+ 2.0 μl of dNTP(2 mM)
<br /><br />
+ 1.0 μl of template (E.coli plasmid 817)
<br /><br />
+ 1.0 μl of oligonucleotide primer PtoA-F*<br /><br />
+ 1.0 μl of oligonucleotide primer PtoA-R*
<br /><br />
+ 18.25 μl of ddH2O
<br /><br />
*The sequences of primer pair PtoA-F and PtoA-R.<br /><br />
PtoA-F 5'-CCACCTGACGTCTAAGAAAC-3'<br /><br />
PtoA-R 5'-ATGATCATCGCCGGCGAATTCAGGC-3' <br /><br />
<br />
2. Set parameters for PCR to amplify desired products.
<br/><br />
<br />
<table><br />
<tr><br />
<td>Temperature</td> <td>Time</td> <td>Cycle</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>5min</td> <td>1</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>55˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>72˚C</td> <td>1min 20sec</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>4˚C</td> <td>7hrs</td> <td>1</td><br />
</tr><br />
</table><br />
<!--那个p的结束,下同--><br />
</p><br />
<br />
<p><br />
<font face="Arial, Helvetica"><br />
<br />
<a name="Restriction" ></a> <br />
</font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Restriction Enzyme Digestion for Verification</font></b></font></h3><br />
To verify whether PstI site in pSB1A3 was successfully mutated to AflII site, we performed restriction enzyme digestion experiments. <br/><br />
<br />
Bacterial plasmids are double-stranded circular DNA molecules and uncut plasmid DNA can be in any of three forms - nicked circular, linear, closed supercoiled. When run on an agarose gel one frequently will see these forms as different bands with closed supercoiled form migrates the fastest, linear form migrates the slowest, and nicked circular migrates in between. If the PStI site was successfully mutated to AflII site, we expected to see increased linear form of pSB1A3 when digested with AflII. SpeI-cut pSB1A3 was served as a positive control. <br /><br />
<br />
<strong>Method</strong><br /><br />
1. Set up Pst I digestion in 1.5 ml eppendorf tubes as described below:<br /><br />
Total: 10μl<br /><br />
+ 0.5μl of Pst I restriction enzyme (company :Takara)<br /><br />
+ 1μl of 10XH buffer<br /><br />
+ 1μl of plasmid DNA<br /><br />
+ 7.5μl of ddH2O <br /><br />
2. Set up Afl II digestion in 1.5 ml eppendorf tubes as described below:<br /><br />
Total: 10μl<br /><br />
+ 0.5μl of Afl II restriction enzyme, (company :Takara)<br /><br />
+ 1μl of 10XM buffer<br /><br />
+ 1μl of plasmid DNA<br /><br />
+ 1.0μl of 0.01% BSA<br /><br />
+ 6.5μl of ddH2O<br /><br />
3. Incubate the eppendorf tubes in 37℃ water bath for 1-2 hr.
<br/><br />
4. Prepare 1% agarose gel in Conical flask. Weigh 0.6 g agarose and add 60 ml 1x TAE (diluted from 50x TAE). Cover the Conical flask with silver paper to avoid the loss of water vapor. Place the Conical flask in the microwave and microwave for 1 minute with a middle power. Take it out and shake gently till the solution is homogeneous,(BE CAREFUL to watch the solution closely when shake it–it superheats and can boil over and cause severe burns). Continue microwave and swirl until solution is seen clear and homogeneous with no existence of solid. After cool down the agarose gel briefly, add 3 μl of Gelred (10000x ) and mix well. Pour the agarose gel in gel casting apparatus and insert combs.<br/><br />
5. By inserting the pipette tip below the TAE liquid and into the well, add 5 μl of 1kb DNA ladder solution to first (and last if desired) well, skip one well, then begin adding the 5μl of digested DNA solutions mixed with 1 μl loading buffer (6x) to the wells.
<br/><br />
6. Place the cover on the electrophoresis unit, plug into the power source, and turn on voltage to 120V, set time to 30 minutes, and press the start button twice,until the bubbles are seen. DNA separation can be observed as time goes on by turning off the power supply then gently removing the basin from the electrophoresis unit (be careful not to let the gel slip out of the basin) and placing on the UV transilluminator to see DNA bands. <br/><br />
7. When the desired level of separation is obtained, the basin can be placed on the transilluminator for picture taking(Of the absence of transilluminator,we use camera to take pictures with the UV light ).
<br/><br />
8. Cut the gel of specific position and collect it in tubes that have measured weight.
<br/><br />
9. Use DNA Gel Extraction Ki to purify the plasmid DNA mutant-pSB1A3.<br />
RPF (SpeI/AflII-digested), GFP (SpeI/AflII-digested) fragments were ligated into this vector, which was followed by insertion of designed terminator sequences between RFP and GFP, respectively.</p><br />
<font face="Arial, Helvetica"><br />
<img src="https://static.igem.org/mediawiki/2012/5/59/11.png" alt="" class="img_fl img_border" align="left"/> <br />
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br />
(Figure 1 : This figure shows that the site-directed mutagenesis succeed ,we successfully change a restriction enzyme cutting site named Pst I to Afl II. Lane 1 represents the plasmid mutant-pSB1A3, lane 2 shows that the mutant-pSB1A3 cannot be digested by restriction enzyme Spe I ,lane 3 shows that mutant-pSB1A3 can be digested by restriction enzyme Afl II.) <br />
</p><br />
<br />
<p><br />
<a name="Media"></a></font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Media Preparation</font></b></font></h3><br />
For all experiments involving the bacterial biomass and experimentation, proper media is chosen to grow the cells. Commonly,we use Lysogeny broth media for <em>E. coli</em>. The following is the media compositions and their quantities.<br /><br />
<strong>Method:</strong><br/><br />
<br />
1.Prepare the Lysogeny Broth (LB) liquid media (1 L) as indicated below: <br/><br />
+ Bacto-Tryptone - 10 g<br/><br />
+ NaCl - 10 g<br/><br />
+ Yeast Extract - 5 g<br/><br />
Add ddH2O and 5mmol/L Tris Buffer in a measuring cylinder to ensure accuracy, to make a total of 1 liter and pH is 8.0.<br/><br />
2.Prepare the Lysogeny Broth (LB) solid media (1 L) as indicated below:<br/><br />
+ Bacto-Tryptone - 10 g<br/><br />
+ NaCl - 10 g<br/><br />
+ Yeast Extract - 5 g<br/><br />
+ Difco Agar - 15g<br/><br />
Add ddH2O and 5mmol/L Tris Buffer in a measuring cylinder to ensure accuracy, to make a total of 1 liter and pH is 8.0.<br/><br />
3. Autoclaving<br/><br />
Autoclave at 121 °C for 60 minutes. After the media cooling down enough, antibiotics Ampicillin(100mg of Ampicillin per 1ml of the media) are added. At last the media are poured 15ml on each plate and become solid.Store the plate at 4℃ refrigerator.<br />
</p><br />
<br />
<p><br />
<font face="Arial, Helvetica"><br />
<br><br />
<a name="Bacterial"></a></font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Bacterial Transformation</font></b></font></h3><br />
<font face="Arial, Helvetica"><br />
Transformation is commonly used to introduce recombinant plasmid DNA into bacterial strains which can transform naturally or can be made competitive for transformation by artificial means. The purpose of this technique is to introduce a recombinant plasmid DNA into a bacterial strains and to use bacteria strains to amplify the plasmid mutant-pSB1A3 for further plasmid construction.<br />
<br />
<strong>Method</strong><br /><br />
1. Take out an appropriate number of tubes that contain competent cells(100μl ) from the freezer. Immediately place the tubes on ice, so that all but the cap is surrounded by ice. Allow the cells to thaw on ice for 2-5 min.<br /><br />
2. Visually check the cells to see whether they have thawed and gently flick the cells 1-2 times to evenly resuspend the cells.<br /><br />
3. Add 10μl PCR products(mini-prep purified) to the competent cells DH-5α. Stir gently to mix and return the tube to the ice, making sure that the tube is surrounded by ice except for the cap. Repeat for additional two times for the same samples.<br /><br />
4. Incubate the tubes on ice for 30 min.<br /><br />
5. Place the tubes in a 42°C water bath for exactly 90 sec; do not shake.<br /><br />
6. Place the tubes on ice for 2 min to cool down.<br /><br />
7. Add 800 l of room temperature LB medium to each tube.<br /><br />
8. Shake the tubes vigorously at 37<a name="OLE_LINK2" id="OLE_LINK2"></a><a name="OLE_LINK1" id="OLE_LINK1">°C</a> for 45-60 min.<br /><br />
9. Centrifuge the tubes at 3K RPM for 1 min. Discard the supernatant liquor and leave 100-200 μl of the mixtures.Mix the contents and spread the whole liquid on LB agar plates containing the appropriate antibiotic ampicillin for the plasmid.<br /><br />
10. Place the plates on the bench for several min to allow excess liquid to be absorbed, and then invert and incubate overnight at 37°C (12-16 h).</p><br />
<br/><br />
</p><br />
<br />
<p><br />
<a name="Colony"></a></font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Colony PCR for Verification</font> </b></font></h3><br />
<font face="Arial, Helvetica"><br />
Colony PCR is used to identify and select cell colonies that have the correct plasmid inserted. The procedure is a way to do several PCR operations on cell colonies in parallel, to evaluate the results and select the corresponding positive cell colonies. After an overnight growth of E.coli, we can pick up several colonies from the plate and do a colony PCR verification. Besides, the colonies we choose and should also be stored, we can incubate these colonies in one plate after every colony has been marked.<br />
<br />
<strong>Method</strong><br /><br />
1. Prepare the sample reaction as indicated below:<br /><br />
Total: 25μl <br /><br />
+ 0.25 μl of Ex Taq polymerase (company:Takara)<br /><br />
+ 2.5 μl of 10× Taq reaction buffer<br /><br />
+ 1.0 μl of R-NPS-F*<br /><br />
+ 1.0 μl of G-SXA-R*<br /><br />
+ 1.0 μl of plasmid mutant-pSB1A3<br /><br />
+ 2.0 μl of dNTP( 25mM ) <br /><br />
+ 17.25 μl of ddH2O <br /><br />
Note: The sequences of primers R-NPS-F , G-SXA-R <br /><br />
R-NPS-F 5'-TATAGCGGCCGCCTTAAGTAAGTAAGAGTATACG-3' <br /><br />
G-SXA-R 5'-TCTCCGACTTAAGGGATCCTATAAACGCAG-3'<br /><br />
<br />
2. Set parameters for PCR to amplify desired products.
<br />
<br />
<table><br />
<tr><br />
<td>Temperature</td> <td>Time</td> <td>Cycle</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>5min</td> <td>1</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>55˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>72˚C</td> <td>1min 20sec</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>4˚C</td> <td>7hrs</td> <td>1</td><br />
</tr><br />
</table><br />
<br />
3. Electrophorese the total system and observe the lane separation. <br />
<br><br />
<a name="Culture"></a></font><br />
<br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Cultivate the Bacteria</b></font></h3><br />
<font face="Arial, Helvetica"><p>According to the results of the PCR detection, positive colonies are chosen and transferred them to 5ml LB liquid media ( 5μl of ampicillin added) stored in 12.5ml centrifuge tubes. Put the centrifuge tubes in 37℃ gas bath overnight. <br />
<br><br />
<a name="Plasmid"></a><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Plasmid DNA Isolation</b></font></h3><br />
Use E.Z.N.A.TM Plasmid Mini I to realize plasmid DNA isolation.<br /><br />
<strong>Method</strong><br/><br />
<br />
<ol><br />
<li>Transfer 5 ml of overnight culture into a 1.5-ml eppendorf tube labeled with group number. </li><br />
<li>Centrifuge the sample at max. speed of desk top centrifuge and RT for 1min to pellet the cells.</li><br />
<li>Discard the supernatant. Remove as much of the supernatant as possible without disturbing the cell pellet. </li><br />
<li>Repeat step 1 and 2 twice. </li><br />
<li>Resuspend the pellet completely in 250 ml of Solution I (containing RNase A) by vortexing the samples vigorously . No clumps should be visible in the tube. </li><br />
<li>Add 250 ml of Solution II and mix the sample by gently inverting the tube 4 to 6 times. Do not vortex or shake the sample vigorously. The bacterial suspension should begin to clear which have lysed the bacterial cells in this step. <strong>Warning: </strong>Do not stop here for more than five min, as the high pH hurts your DNA! </li><br />
<li>Add 350 ml of Solution III and mix by gently inverting the tube 4 to 6 times until a flocculent white precipitate forms. Do not shake vigorously, as it might break the genomic DNA. </li><br />
<li>Centrifuge at maximum speed for 10 min at room temperature to pellet the cell debris. You should see a white precipitate in the tube after the centrifugation. </li><br />
<li>While the samples are centrifuging, for each sample, label a clean HiBind Miniprep Column which is to assembled in a 2-ml collection tube</li><br />
<li>Apply the supernatants from step 8 to the columns. </li><br />
<li>Centrifuge at maximum speed for 1 min at RT. Discard the flow-through in the collection tube. </li><br />
<li>Add 500 ml of Buffer HB to wash the Hibind Miniprep Column. Centrifuge at maximum speed for 1 min at RT. Discard the flow-through in the collection tube. </li><br />
<li>Wash the column by adding 700 ml of DNA Wash Buffer diluted with absolute ethanol. Centrifuge at maximum speed for 1 min at room temperature and discard the flow-through.</li><br />
<li>Then centrifuge the tubes again for 2 min to remove all the moisture.</li><br />
<li>Place the column in a clean 1.5 ml eppendorf tube that is labeled with the plasmid name and group number. To elute the DNA, add 50 ml of Elution Buffer to the center of each column. Let the samples stand for 2 or more minutes at RT, and then centrifuge for 1 min. The sample in the centrifuge tube (bottom) is your plasmid DNA. </li><br />
<li>Discard the column and save the sample in the eppendorf tube by placing it in the freezer (-20°C). </li><br />
</ol><br />
</p><br />
<br />
<br />
<a name="#Restriction_2" ></a> <br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Restriction Enzyme Digestion and Electrophoresis</font></b></font></h3><br />
<font face="Arial, Helvetica"><br />
<p>Because the colony PCR test is so sensitive and affect markedly by environment factors. So we do a restriction enzyme digestion to ensure that the isolated plasmid is the site-directed mutated plasmid.<br /><br />
<strong>Method</strong><br /><br />
1. Prepare the control reaction as indicated below:<br /><br />
Total: 10μl<br /><br />
+ 0.5μl of Pst I restriction enzyme(company :Takara)<br /><br />
+ 1μl of 10XH buffer<br /><br />
+ 1μl of plasmid DNA<br /><br />
+ 7.5μl of ddH2O <br /><br />
2. Prepare the sample reaction as indicated below:<br /><br />
Total: 10μl<br /><br />
+ 0.5μl of Afl II restriction enzyme, (company :Takara)<br /><br />
+ 1μl of 10XM buffer<br /><br />
+ 1μl of plasmid DNA<br /><br />
+ 1.0μl of 0.01% BSA<br /><br />
+ 6.5μl of ddH2O <br /><br />
3. Electrophorese the total system and observe the lane separation.</p><br />
<br/><br />
<br />
<a name="Amplify"></a> <br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Polymerase Chain Reaction(GFP &amp; RFP) and Electrophoresis</font></b></font></h3><br />
<font face="Arial, Helvetica"><br />
<p>GFP and RFP DNA fragments are the insert which need to be ligate to the plasmid mutant-pSB1A3. Do a PCR amplification can get enough quantities for the following reactions.<br /><br />
<strong>Method </strong><br /><br />
1.Prepare the sample reaction as indicated below:<br /><br />
Total: 100μl ( PCR <a name="OLE_LINK37" id="OLE_LINK37"></a><a name="OLE_LINK36" id="OLE_LINK36">amplification</a> of GFP fragments) <br /><br />
+ 1.0μl of Taq DNA polymerase,#EP0402<br /><br />
+ 10μl of 10XTaq buffer <br /><br />
+ 10μl of MgCl2(25mM)<br /><br />
+ 10μl of dNTP(2mM)<br /><br />
+ 2μl of G-SXA-R* <br /><br />
+ 2μl of G-SXA-F*<br /><br />
+ 4μl of DNA template<br /><br />
+ 61μl of ddH2O <br /><br />
Total: 100μl(PCR amplification of RFP fragments) <br /><br />
+ 1.0μl of Taq DNA polymerase, EP0402<br /><br />
+ 10μl of 10XTaq buffer <br /><br />
+ 10μl of MgCl2(25mM)<br /><br />
+ 10μl of dNTP(2mM)<br /><br />
+ 2μl of R-NPS-R* <br /><br />
+ 2μl of R-NPS-F*<br /><br />
+ 4μl of DNA template<br /><br />
+ 61μl of ddH2O <br /><br />
<br />
Note: The sequences of primers R-NPS-F , R-NPS-R , G-SXA-F ,G-SXA-R <br /><br />
R-NPS-F 5'-TATAGCGGCCGCCTTAAGTAAGTAAGAGTATACG-3' <br /><br />
R-NPS-R 5'-CGGAGACTAGTCTGCAGATCACATAAGTAAAGTGATAATC-3' <br /><br />
G-SXA-F 5'-CTAGACTAGTTCTAGAGGCGGACTCACTATAGA-3' <br /><br />
G-SXA-R 5'-TCTCCGACTTAAGGGATCCTATAAACGCAG-3'<br /><br />
<br />
<p> 2. Set parameters for PCR to amplify desired products.
</p><br />
<br />
<table><br />
<tr><br />
<td>Temperature</td> <td>Time</td> <td>Cycle</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>5min</td> <td>1</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>55˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>72˚C</td> <td>1min 20sec</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>4˚C</td> <td>7hrs</td> <td>1</td><br />
</tr><br />
</table><br />
</p><br />
<p>3. Use DNA Gel Extraction Kit to purify the GFP and RFP DNA fragments after the Electrophoresis.</p><br />
<br />
<p><font face="Arial, Helvetica"><img src="https://static.igem.org/mediawiki/2012/7/72/111.png" alt="" class="img_fl img_border" align="left"/> </font></p><br />
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br />
<p>(Figure 2 : This figure shows that The PCR reaction system can amplify large quantities of GFP and RFP DNA fragments. The digestion based on the GFP and RFP DNA fragments can be done to prepare for the ligation. Lane 1 represents the template E.coli 817 can amplify the GFP and RFP DNA fragments , lane 2 represents the template E.coli 817(355.5) can also amplify the GFP and RFP DNA fragments.)<br/><br/><br />
<font face="Arial, Helvetica"><br/><br />
<a name="Restriction_Enzyme"></a><br />
</font></p><br />
<font face="Arial, Helvetica"> </font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Double Restriction Enzyme Digestion and Electrophoresis.</font></b></font></h3><br />
<font face="Arial, Helvetica"><br />
<p>Use specific restriction enzymes to digest plasmid mutant-pSB1A3,GFP and RFP to get sticky ends and purify the DNA fragment after the Electrophoresis.<br /></p><br />
<p> <strong>Method:</strong><br/><br />
</font><br />
<ul><br />
<li> Digestion of plasmid mutant-pSB1A3 </li><br />
</ul><br />
<p>Prepare the sample reaction as indicated below:<br /><br />
Total: 50μl <br /><br />
+ 3.0μl of Not I restriction enzyme, #ER0591<br /><br />
+ 3.0μl of Afl II restriction enzyme, #ER0831<br /><br />
+ 5.0μl of 10X buffer O <br /><br />
+ 1.0μl of mutant-pSB1A3 plasmid <br /><br />
+ 37.0μl of ddH2O<br /><br />
2. Digestion of PCR product GFP<br /><br />
Prepare the sample reaction as indicated below:<br /><br />
Total: 50μl <br /><br />
+ 3.0μl of Not I restriction enzyme, #ER0591<br /><br />
+ 3.0μl of Spe I restriction enzyme, #ER1251 <br /><br />
+ 5μl of 10X buffer Tango<br /><br />
+ 10μl of PCR products GFP<br /><br />
+ 29μl of ddH2O<br /><br />
3. Digestion of PCR product RFP<br /><br />
Prepare the sample reaction as indicated below:<br /><br />
Total: 50μl <br /><br />
+ 3.0μl of Afl II restriction enzyme, #ER0831<br /><br />
+ 3.0μl of Spe I restriction enzyme, #ER1251 <br /><br />
+ 5μl of 10X buffer Tango<br /><br />
+ 10μl of PCR products RFP<br /><br />
+ 29μl of ddH2O<br /><br />
4. Put the tubes in 37℃ environment for 4-8 hours <br /><br />
5. Use DNA Gel Extraction Kit to purify the mutant-pSB1A3 fragment, GFP and RFP after digestion and named them by mutant-pSB1A3 (NA) ,GFP(NS) and RFP(AS) after the Electrophoresis.</p><br />
</p><br />
<br />
<font face="Arial, Helvetica"><br><br />
<a name="Ligayion"></a> </font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Ligation</font></b></font></h3><br />
<font face="Arial, Helvetica"><br />
<p>Ligation is the process that target DNA gene is inserted into a plasmid. Both the vector and insert are prepared to have the sticky ends. These two kinds of DNA pieces are placed in a reaction tube and the proper DNA ligase, buffer, and cofactors are added for the reaction to take place. When done properly, the ligation will result in a successful combination of the insert and plasmid into one plasmid.Based on the digestion of mutant-pSB1A3,GFP and RFP DNA fragments,also ligation can be done. We ligate mutant-pSB1A3 vector and sticky GFP and RFP DNA fragments to construct an new plasmid mutant-pSB1A3-GR. <br /><br />
1. Prepare the control reaction as indicated below:<br /><br />
Total: 10μl <br /><br />
+ 1.0μl of plasmid mutant-pSB1A3 (NA) <br /><br />
+ 3.0μl of GFP(NS) <br /><br />
+ 3.0μl of RFP(AS) <br /><br />
+ 2.0μl of T4 DNA Ligase,#EL0011 <br /><br />
+ 1.0μl of 10XT4 Ligase buffer<br /><br />
Note: GFP(NS) means the product of GFP DNA fragments digested by restriction enzyme Not I and Spe I. <br /><br />
2. Prepare the sample reaction as indicated below:<br /><br />
Total: 10μl <br /><br />
+ 1.0μl of plasmid mutant-pSB1A3 (NA) <br /><br />
+ 2.0μl of T4 DNA Ligase, #EL0011 <br /><br />
+ 1.0μl of 10XT4 Ligase buffer<br /><br />
+ 6.0μl of ddH2O<br /><br />
3. Put the tubes in 22℃ water bath, react for 8-12 hours. <br><br />
<a name="Bacterial_Transformation"></a> </font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Bacterial Transformation</font></b></font></h3><br />
<font face="Arial, Helvetica"><br />
<p>Transform the ligation products into the DH-5α competent cells, put the plate on 37℃ gas bath for 12-16 hours. </p><br />
<br><br />
<a name="Bacterial_Colony"></a> </font><br />
<h3><font face="Arial, Helvetica"><b><font color="#0000FF">Bacterial Colony PCR</font></b> </font></h3><br />
<font face="Arial, Helvetica"><br />
<p>Colony PCR is used to identify and select cell colonies that have the correct plasmid insert. This procedure is a way to do several PCR operations on cell colonies in parallel, to evaluate the results and select the corresponding good cell colonies. After an overnight growth of E.coli, we can pick up some colonies from the plate and do a colony PCR verification. Besides, the colonies we choose and should also be stored, we can incubate these colonies in one plate after every colony has been marked.<br /><br />
<strong>Method</strong><br /><br />
1. Prepare the sample reaction as indicated below:<br /><br />
Total: 20μl <br /><br />
+ 0.25 μl of Ex Taq polymerase,#EP0402 <br /><br />
+ 2.0 μl of 10× Taq reaction buffer<br /><br />
+ 1.0 μl of R-NPS-F* <br /><br />
+ 1.0 μl of G-SXA-R*<br /><br />
+ 5.0 μl of bacterial colony<br /><br />
+ 2.0 μl of dNTP( 25mM ) <br /><br />
+ 8.75 μl of ddH2O <br /><br />
Note: The sequences of primers R-NPS-F , R-NPS-R , G-SXA-F ,G-SXA-R <br /><br />
R-NPS-F 5'-TATAGCGGCCGCCTTAAGTAAGTAAGAGTATACG-3' <br /><br />
R-NPS-R 5'-CGGAGACTAGTCTGCAGATCACATAAGTAAAGTGATAATC-3' <br /><br />
G-SXA-F 5'-CTAGACTAGTTCTAGAGGCGGACTCACTATAGA-3' <br /><br />
G-SXA-R 5'-TCTCCGACTTAAGGGATCCTATAAACGCAG-3'<br /><br />
<br />
<p> 2. Set parameters for PCR to amplify desired products.
</p> <br />
<table><br />
<tr><br />
<td>Temperature</td> <td>Time</td> <td>Cycle</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>13.5min</td> <td>1</td><br />
</tr><br />
<tr><br />
<td>94˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>55˚C</td> <td>1min</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>72˚C</td> <td>1min 20sec</td> <td>30</td><br />
</tr><br />
<tr><br />
<td>4˚C</td> <td>7hrs</td> <td>1</td><br />
</tr><br />
</table><br />
<br />
</font><br />
<p> 3. Electrophorese the total system and observe the lane separation. </p><br />
<br />
<p> <img src="https://static.igem.org/mediawiki/2012/0/07/1111.png" alt="" class="img_fl img_border" align="left"/> </p><br />
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br />
<p>(Figure 3: The lane on the figure are 2k DNA fragments, it shows the GFP and RFP DNA fragments are ligated to the vectors which were isolated from the bacterial colonies.) </p><br />
<br />
<p><font face="Arial, Helvetica"><br><br />
<a name="Culture_the"></a><br />
</font></p><br />
<font face="Arial, Helvetica"><br />
</font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Cultivate the Bacteria</b></font></h3><br />
<font face="Arial, Helvetica"><p>According to the results of the PCR detection, we choose positive colonies and transfer them to 5ml LB liquid media ( 5μl of ampicillin has added) stored in 12.5ml centrifuge tubes. Put the centrifuge tubes in 37℃ gas bath overnight. </p><br />
<br />
<p><font face="Arial, Helvetica"><a name="Plasmid_DNA"></a> </font></p><br />
<font face="Arial, Helvetica"> </font> </font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Plasmid DNA Isolation</b></font></h3><br />
<p><font face="Arial, Helvetica">Use E.Z.N.A.TM Plasmid Mini I to isolate the constructed plasmid mutant-pSB1A3-GR. </font></p><br />
<br />
<p><font face="Arial, Helvetica"><a name="Restriction_Enzyme" ></a> </font></p><br />
<font face="Arial, Helvetica"> </font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Restriction Enzyme Digestion and Electrophoresis</b></font></h3><br />
<p>From the last step, we got the certain quantities of isolated plasmids. In this step, we do two restriction enzyme digestion reactions, one to prove that the plasmid is construct correctly ( mutant-pSB1A3-GR ), one to get sticky ends preparing for the ligation.<br /><br />
<strong>Method</strong></p><br />
<p>1.Restriction Enzyme Digestion to prove that plasmid is constructed correctly</p><br />
<br />
<p align="left">1.1. Prepare the sample reaction as indicated below:<br /><br />
Total: 10μl<br /><br />
+ 1.0μl of Not I restriction enzyme,#ER0591<br /><br />
+ 1.0μl of Spe I restriction enzyme,#ER1251 <br /><br />
+ 2.0μl of Buffer Tango( 10X )<br /><br />
+ 1.5μl of plasmid mutant-pSB1A3-GR<br /><br />
+ 14.5μl of ddH2O <br /><br />
1.2. Electrophorese the total system and observe the lane separation. </p><br />
<br />
<p>2. Restriction Enzyme Digestion to get sticky ends preparing for the ligation.</p><br />
<br />
<p align="left">2.1. Prepare the sample reaction as indicated below:<br /><br />
Total: 50μl<br /><br />
+ 5.0μl of Pst I restriction enzyme, #ER0611<br /><br />
+ 5.0μl of Xba I restriction enzyme, #ER0681 <br /><br />
+ 3.0μl of Buffer Tango( 10X )<br /><br />
+ 5.0μl of plasmid mutant-pSB1A3-GR<br /><br />
+ 32.0μl of ddH2O <br /><br />
2.2. Electrophorese the total system and observe the lane separation.<br /><br />
2.3. Cut the gel of specific position and collect it in tubes that have measured weight. <br /><br />
2.4. Use DNA Gel Extraction Ki to purify the plasmid DNA mutant-pSB1A3-GR(PX) .<br /><br />
Note: Mutant-pSB1A3-GR(PX) means plasmid Mutant-pSB1A3-GR digested by Pst I and Xba I.</p><br />
<br />
<br />
<p align="left"><strong><img src="https://static.igem.org/mediawiki/2012/d/d8/11111.png" alt="" class="img_fl img_border" align="left" /></strong></p><br />
<p>(Figure 4 : The double digestion of mutant-pSB1A3 forms a linear DNA fragments and it runs slower than circle DNA fragments. This suggest that the double digestion of mutant-pSB1A3 works in a high efficiency, and desired sticky ends are formed.1,3,5 are plasmids digested by restriction enzyme Pst I and Xba I from different colonies, 2,5,6 are pure plasmid mutant-pSB1A3-GR, 7 is the plasmid mutant-pSB1A3. )</p><br />
<p align="left">&nbsp;</p><br />
<p><font face="Arial, Helvetica"><a name="Ligation1" ></a> </font></p><br />
<font face="Arial, Helvetica"> </font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Ligation</b></font></h3><br />
<p>Ligation is the process that target DNA gene is inserted into a plasmid. Both the vector and insert are prepared to have the sticky ends. These two kinds of DNA pieces are placed in a reaction tube and the proper DNA ligase, buffer, and cofactors are added for the reaction to take place. When done properly, the ligation will result in a successful combination of the insert and plasmid into one plasmid.Based on the digestion of mutant-pSB1A3-GR, terminator DNA fragments,ligation can be done. We ligate mutant-pSB1A3-GR vector and sticky terminator DNA fragments to construct an new plasmid mutant-pSB1A3-GR-t.By detecting the quantities of GFP and RFP, terminator efficiency can be calculated. <br /><br />
1. Prepare the control reaction as indicated below:<br /><br />
Total: 10μl <br /><br />
+ 1.0μl of plasmid mutant-pSB1A3 (NA) <br /><br />
+ 6.0μl of terminator<br /><br />
+ 2.0μl of T4 DNA Ligase , #EL0011 <br /><br />
+ 1.0μl of 10XT4 Ligase buffer<br /><br />
2. Put the tubes in 22℃ water bath, react for 8-12 hours. </p><br />
<p><font face="Arial, Helvetica"><a name="Bacteria" id="Culture_the"></a> </font></p><br />
<font face="Arial, Helvetica"> </font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Bacteria transformation</b></font></h3><br />
<p>Transform the ligation products into the DH-5α competent cells, put the plate on 37℃ gas bath for 12-16 hours. </p><br />
<p><font face="Arial, Helvetica"><a name="Cultivate"></a> </font></p><br />
<font face="Arial, Helvetica"> </font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Cultivate the Bacteria</b></font></h3><br />
<p>According to the results of the PCR detection, we choose positive colonies and transfer them to 5ml LB liquid media ( 5μl of ampicillin has added) stored in 12.5ml centrifuge tubes. Put the centrifuge tubes in 37℃ gas bath overnight. </p><br />
<br />
<p><font face="Arial, Helvetica"><a name="Flow" ></a> </font></p><br />
<font face="Arial, Helvetica"> </font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Flow Cytometer Analysis</b></font></h3><br />
<p>Flow cytometry is a laser based, biophysical technology employed in cell counting, sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second. </p><br />
<p>Each fluorophore has a characteristic peak excitation and emission wavelength, and the emission spectra often overlap. Consequently, the combination of labels which can be used depends on the wavelength of the lamp(s) or laser(s) used to excite the fluorochromes and on the detectors available. It is practical that the expression of RFP and GFP can be measured at the same time.</p><br />
<p><strong>Method:</strong><br/><br />
1. Materials:<br/><br />
&nbsp;&nbsp;1.1. NaCl solution( 0.9% ,M/V)<br/><br />
&nbsp;&nbsp;1.2. 75% ethanol<br/><br />
2. Procedures:<br/><br />
&nbsp;&nbsp;2.1. Bacterium are harvested by centrifuge at 10000g for 30s, then discard the supernatant. Repeat this step again until we got adequate bacterium.<br/><br />
&nbsp;&nbsp;2.2. Suspend the bacterium With NaCl solution(0.9%) and vibrate the centrifuge tubes until the bacterium are distributed homogeneous.<br/><br />
&nbsp;&nbsp;2.3. Load the bacteria containing pSB1A3 to Flow Cytometer (Beckman), set the parameters. Measure the GFP-FL1 and RFP-FL2 by Cytometer. </p><br />
<br />
<p><font face="Arial, Helvetica"><a name="Fluorescence"></a> </font></p><br />
<font face="Arial, Helvetica"> </font><br />
<h3><font color="#0000FF" face="Arial, Helvetica"><b>Fluorescence Microscope</b></font></h3><br />
<p>A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence reflection and absorption to study properties of organic or inorganic substances and generate an image.Fluorescence microscope detection are used to confirm the expression of GFP and RFP which are illuminated with light of a wavelength which excites fluorescence in the sample.<br />
</p><br />
<p><strong>Method</strong><br /><br />
Add 10μl of bacteria solution to micro slide and cover with coverslip.<br />
Placed the micro slide on the Fluorescence Microscope. Set parameters to detect GFP and RFP. <br/><br />
Generate an image of fluorescence protein and save it.</p><br />
<br />
<br />
<br> <br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction
Team:SUSTC-Shenzhen-B/lab introduction
2012-10-26T21:17:01Z
<p>Water: </p>
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.widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li .smalldate{display:block;font-size:11px;font-style:italic;overflow:hidden}#footercontainer{background:#3f4432;-webkit-border-bottom-left-radius:7px;-webkit-border-bottom-right-radius:7px;-moz-border-radius-bottomleft:7px;-moz-border-radius-bottomright:7px;border-bottom-left-radius:7px;border-bottom-right-radius:7px}#footer{padding:25px 0 25px 0;color:#bbb9a1;font-weight:400;font-family:'Open Sans',sans-serif;font-size:13px}#footer a,#footer a:visited{color:#bbb9a1}<br />
<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
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* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
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*<br />
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<h1><p>Introduction to the Lab Work</p></h1><br />
<br />
<br />
<h3 class="STYLE10">Objective</h3><br />
<p>The goal of our wet lab work is to validate our software prediction. Since the terminator efficiency data available in literatures is very limited, we designed 100 terminators through the knowledge of terminator structure, and designed an experiment to measure terminator efficiency. By analyzing experiment results, we can adjust the parameters in our model to make it more accurate.</p><br />
<br />
<h3 class="STYLE10">1, How to measure efficiency:</h3><br />
<br />
<img src="https://static.igem.org/mediawiki/2012/6/62/Project.introduction.3JPG.JPG" alt="" class="img_fl img_border" /><br />
<p>The figure above shows the plasmid designed to measure terminator efficiency. The terminator to be characterized would be flanked by gene sequences of two fluorescent proteins, GFP and RFP. By using flow cytometry, we could measure the fluorescence strength of GFP and RFP, so as to measure the terminator efficiency. </p><br />
<br />
The terminator efficiency of a terminator is calculated with the formula:<br />
<p> E = 1- S/T </p><br />
where S is the fluorescence strength of GFP with terminator, and T is the fluorescence strength of GFP without terminator, which is the baseline strength.<br />
<br />
<br />
<br />
<br />
<h3 class="STYLE10">2. Plasmid Construction</h3><br />
<p>A goal of synthetic biology is to standardize the form of genetic components to prevent structural elements of components from changing with reactions. It provides a standard method of assembling genetic components using specified prefixes and suffixes. Prefixes include EcoRⅠ and XbaⅠ; suffixes include SpeⅠ and PstⅠ.<br />
<p>The recognition site of XbaⅠ:</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/2e/Xba1.JPG" class="img_fl img_border" /><br />
<p>(Figure 3)</p><br />
<p>The recognition site of SpeⅠ:</p><br />
<img src="https://static.igem.org/mediawiki/2012/7/77/Spe1.JPG" class="img_fl img_border" /> <br />
<p>(Figure 4)</p><br />
<p>The same cohesive terminus enable them to connect with each other. However, after the combination, neither XbaⅠnor SpeⅠis able to recognize this sequence. Through this way we could achieve the goal of building BioBricks. </p><br />
<p>We can see from the picture(Figure 2) that there are 4 standard restriction enzyme cutting sites: <br />
EcoRⅠ, XbaⅠ, SpeⅠ,PstⅠ. </p><br />
</p><br />
<p>(1) As we will use pstⅠto be the restriction cutting site of terminators, if we want to ligase GFP to the plasmid, this restriction cutting site should be mutated.<br />
<p>The recognition site of PstⅠ:</p><br />
<img src="https://static.igem.org/mediawiki/2012/c/cc/Pst1.JPG" class="img_fl img_border" /> <br />
<p>(Figure 5)</p><br />
<p>The recognition site of AflⅡ:</p><br />
<img src="https://static.igem.org/mediawiki/2012/c/cc/Afl2.JPG" class="img_fl img_border" /> <br />
<p>(Figure 6)</p><br />
<p> Since AflⅡis similar to PstⅠ,they just have 2 different base pairs, we used these primers:</p><br />
<p>PtoA-F</p><br />
<p>5'-CCACCTGACGTCTAAGAAAC-3'</p><br />
<p>PtoA-R</p><br />
<p>5'-CTTAAGCGGCCGCTACTAGTA-3'</p><br />
<p>to mutate the vector.</p><br />
</p><br />
<br />
<p>(2) After the mutation succeeded, the next step is to connect sequences of GFP & RFP to the vector. These sequences were built by PCR extension.</p><br />
<p>To digest GFP sequence, we used SpeⅠand AflⅡ. We also digested the vector with the same restriction enzyme cutting sites. During the process of base complementary pairing, GFP sequence could be ligated onto the vector with the help of T4 DNA ligase. At the same time, the recognition site of PstⅠ would be added to the vector.</p><br />
<p>To digest RFP sequence, we used NotⅠ and SpeⅠ. We also digested the vector with the same restriction enzyme cutting sites. During the process of base complementary pairing, RFP sequence could be ligated onto vector with the help of T4 DNA ligase. At the same time, the recognition site of XbaⅠwould be added to the vector.</p><br />
<p>We can either connect 3 fragments: GFP, RFP sequences and the plasmid at the same time, or connect 2 fragments first and then connect the other one. However, when carrying out the second scheme, we should decide which one to be connected first. According to the picture, GFP should be the one.</p><br />
<br />
<p>Besides:</p><br />
<p>We designed these primers to amplify RFP.</p><br />
<p>R-NPS-F</p><br />
<p>5'-TATAGCGGCCGCCTTAAGTAAGTAAGAGTATACG-3' </p> <br />
<p>R-NPS-R</p><br />
<p>5'-CGGAGACTAGTCTGCAGATCACATAAGTAAAGTGATAATC-3'</p><br />
<p>We designed these primers to amplify GFP.</p><br />
<p>G-SXA-F</p><br />
<p>5'-CTAGACTAGTTCTAGAGGCGGACTCACTATAGA-3' </p><br />
<p>G-SXA-R</p><br />
<p>5'-CCGACTTAAGGGATCCTATAAACGCAG-3' </p><br />
<p>(3) Since we built up the vector through the steps before, we decided to use XbaⅠ,PstⅠto digest the vector.</p> <br />
<p>The terminators were built by PCR extension.</p><br />
<p>Then we can ligated terminator into the backbone and transformed into competent cells.</p><br />
<p>Characterization devices testing the performance of the terminators utilized fluorescent proteins to measure input and output and altered the arabinose transport system to control inputs. The fluorescence produced by the characterization devices were then measured using flow cytometry to calculate the termination efficiency of the terminators. </p><br />
<br />
<br />
<h3 class="STYLE10">3. Fluorescence strength quantification</h3><br />
<p>We used flow cytometer to measured the fluorescence strength. Laser was used to be the luminous source and then radiated perpendicular to the sample flow. Under the irradiation of laser, cells expressed fluorescent protein. We calculated the average expression level of those cells and got the average strength of GFP. We also used fluorescence microscope to take a picture of bacterial culture. </p><br />
<br />
<h3 class="STYLE10">4. Agreement with theoretical prediction</h3><br />
<P> According to our experiment measured terminator efficiencies and our software predicted d scores, we created a fit curve to relate all the data. The corelation coefficient is 0.8. So we have achieved a good agreement between experimental efficiency and TTEC predicted efficiency.</P><br />
<br />
<h3 class="STYLE10">5. Technical Standard</h3><br />
<P>Our terminator efficiency measurement protocol has been submitted to Biobrick foundation as a technical standard(BBF RFC 90).</P><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL
Team:SUSTC-Shenzhen-B/SBOL
2012-10-26T21:15:50Z
<p>Water: </p>
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5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li 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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
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</ul><br />
</li><br />
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<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/SBOL&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/SBOL&amp;action=history">History</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
</nav><!-- nav --><br />
<br />
<br />
<div class="clear"></div><br />
</section><br />
<div class="nav-shadow"></div><br />
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<h1><p>SBOL in Software</p></h1><br />
<p>Synthetic Biology Open Language (SBOL) is a data exchange standard for descriptions of genetic parts, devices, modules, and systems. SBOL is neutral with respect to programming languages and software encoding. By supporting SBOL for reading and writing synthetic biology designs, different software tools can directly communicate and store the same representation of these designs. This removes an impediment to sharing engineered systems and permits other researchers and commercial enterprises to start with an unambiguous representation of the design.</p><br />
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<p>Both our PC offline software and web support SBOL document of terminators. Users can input terminator xml file, then our software will analyze the sequence and output the results. </p><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial_WEB
Team:SUSTC-Shenzhen-B/Tutorial WEB
2012-10-26T21:12:35Z
<p>Water: </p>
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.widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li 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<br />
/* prettyPhoto.css */<br />
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook 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.pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Contributions">Contributions</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Acknowledgement">Acknowledgement</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/Tutorial WEB&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/Tutorial WEB&amp;action=history">History</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/October">October</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/October">October</a></li><br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
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<!-- MAIN CONTENT --><br />
<div id="outermain"><br />
<div id="maincontent"><br />
<section id="mainthecontent"><br />
<br />
<article><br />
<h2>WEB TUTORIAL</h2><br />
<br />
<br><br />
<p>First, please visit:</p><br />
<a href="http://www.terminatorefficiency.com/"> http://www.terminatorefficiency.com/</a><br />
<h3 class="STYLE10">Keyboard input</h3><br />
<br><br />
<p>Type in your sequence.</p><br />
<img src="https://static.igem.org/mediawiki/2012/4/45/TW1.jpg "width="700" height="500" alt="" class="img_fl img_border"><br />
<p>Choose direction and click RUN to get result.</p><br />
<img src="https://static.igem.org/mediawiki/2012/0/00/TW2.jpg "width="700" height="500" alt="" class="img_fl img_border"><br />
<h3 class="STYLE10">SBOL format input</h3><br />
<p>Click SBOL button to input xml file.</p> <br />
<img src="https://static.igem.org/mediawiki/2012/b/b4/TW3.jpg "width="700" height="500" alt="" class="img_fl img_border"><br />
<p>Browse and locate the xml file.</p> <br />
<img src="https://static.igem.org/mediawiki/2012/1/1f/TW4.jpg "width="800" height="500" alt="" class="img_fl img_border"><br />
<p>Wait until upload finished, then choose direction and RUN.</p> <br />
<img src="https://static.igem.org/mediawiki/2012/a/a6/TW5.jpg "width="800" height="500" alt="" class="img_fl img_border"><br />
<br />
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</html></div>
Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial_PC
Team:SUSTC-Shenzhen-B/Tutorial PC
2012-10-26T21:11:55Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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.pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial">Tutorial</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SBOL">SBOL</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">WetLab</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab introduction">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/protocol1">Protocol</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Lab Results</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/safety">Safety</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab.sbol">SBOL Document</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/RFC">Technical Standard</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Team</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/members">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/advisers">Advisers</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/photos">Photo Gallery</a></li><br />
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<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/Tutorial PC&amp;action=edit">Edit</a></li><br />
<li><a href="/wiki/index.php?title=Team:SUSTC-Shenzhen-B/Tutorial PC&amp;action=history">History</a></li><br />
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<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Notebook</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/before.june">Before June</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">July</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/august">August</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/September">September</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/October">October</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Human Practices</a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/hp.intro">Overview</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/open.class">Open Classes</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/high.school.visits">High School Visits</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/SynBio.intro">SynBio Intro</a></li><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/October">October</a></li><br />
</ul><br />
</li><br />
</ul><!-- topnav --><br />
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<article><br />
<h2>PC SOFTWARE TUTORIAL</h2><br />
<br><br />
<p>If you don't have exe file, you can download it<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download"> here</a>.</p><br />
<p>Execute TTEC program</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/20/TP1.JPG " alt="" class="img_fl img_border" /><br />
<h3 class="STYLE10">Keyboard input</h3><br />
<br><br />
<p>TTEC button to input your sequence by keyboard and choose the direction.</p><br />
<img src="https://static.igem.org/mediawiki/2012/7/71/TP2.JPG "width="700" height="380" alt="" class="img_fl img_border"><br />
<p>Click TTEC RUN to get result .</p> <br />
<img src="https://static.igem.org/mediawiki/2012/0/07/TP3.JPG "width="700" height="380" alt="" class="img_fl img_border"><br />
<h3 class="STYLE10">SBOL format input</h3><br />
<p>Click SBOL button to input xml file.</p> <br />
<img src="https://static.igem.org/mediawiki/2012/6/6d/TP4.JPG "width="700" height="380" alt="" class="img_fl img_border"><br />
<p>Choose the direction and run.</p> <br />
<img src="https://static.igem.org/mediawiki/2012/0/0d/TP5.JPG "width="700" height="380" alt="" class="img_fl img_border"><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/Tutorial
Team:SUSTC-Shenzhen-B/Tutorial
2012-10-26T21:07:59Z
<p>Water: </p>
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<br />
/* prettyPhoto.css */<br />
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.pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {<br />
/* Gallery */<br />
png2js("/wiki/images/a/a8/Gallery-js.png", function() {<br />
<br />
//add username to account dropmenu<br />
if ($('#pt-login').html()) {<br />
$('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');<br />
} else {<br />
$('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');<br />
}<br />
<br />
});<br />
});<br />
});<br />
});<br />
<br />
</script><br />
</head><br />
<body><br />
<div id="bodychild"><br />
<div id="outercontainer"><br />
<br />
<!-- HEADER --><br />
<div id="outerheader"><br />
<header id="top"><br />
<br />
<section id="navigation"><br />
<nav><br />
<ul id="topnav" class="sf-menu"><br />
<li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li><br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/comment
Team:SUSTC-Shenzhen-B/comment
2012-10-26T21:06:47Z
<p>Water: </p>
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.pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
var $childUl = $('>ul',this);<br />
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';<br />
$childUl.css(offsetDirection,emWidth);<br />
});<br />
});<br />
<br />
});<br />
};<br />
// expose defaults<br />
$.fn.supersubs.defaults = {<br />
minWidth : 9, // requires em unit.<br />
maxWidth : 25, // requires em unit.<br />
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
};<br />
<br />
})(jQuery); // plugin code ends<br />
<br />
</script><br />
<script type="text/javascript"><br />
// custom.js<br />
jQuery(document).ready(function(){<br />
<br />
//Add Class Js to html<br />
jQuery('html').addClass('js'); <br />
<br />
//=================================== MENU ===================================//<br />
jQuery("ul.sf-menu").supersubs({ <br />
minWidth : 12, // requires em unit.<br />
maxWidth : 17, // requires em unit.<br />
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values<br />
// due to slight rounding differences and font-family <br />
}).superfish(); // call supersubs first, then superfish, so that subs are <br />
// not display:none when measuring. Call before initialising <br />
// containing tabs for same reason.<br />
<br />
//=================================== TABS AND TOGGLE ===================================//<br />
//jQuery tab<br />
jQuery(".tab-content").hide(); //Hide all content<br />
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab<br />
jQuery(".tab-content:first").show(); //Show first tab content<br />
//On Click Event<br />
jQuery("ul.tabs li").click(function() {<br />
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class<br />
jQuery(this).addClass("active"); //Add "active" class to selected tab<br />
jQuery(".tab-content").hide(); //Hide all tab content<br />
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content<br />
jQuery(activeTab).fadeIn(200); //Fade in the active content<br />
return false;<br />
});<br />
<br />
//jQuery toggle<br />
jQuery(".toggle_container").hide();<br />
jQuery("h2.trigger").click(function(){<br />
jQuery(this).toggleClass("active").next().slideToggle("slow");<br />
});<br />
<br />
<br />
});<br />
<br />
</script><br />
<br />
<script type="text/javascript"><br />
/* load png for javascript need canvas (HTML5)*/<br />
function png2js(pngurl, callback){<br />
var canvas = document.createElement("canvas"),<br />
ctx = canvas.getContext("2d");<br />
img = new Image();<br />
<br />
img.style.position = "absolute";<br />
img.style.left = "-10000px";<br />
document.body.appendChild(img);<br />
<br />
img.onload = function() {<br />
var <br />
w = this.offsetWidth,<br />
h = this.offsetHeight;<br />
<br />
canvas.width = w;<br />
canvas.height = h;<br />
canvas.style.width = w+"px";<br />
canvas.style.height = h+"px";<br />
<br />
ctx.drawImage(this, 0, 0);<br />
<br />
var data = ctx.getImageData(0, 0, w, h).data,<br />
a = [],<br />
len = data.length,<br />
p = -1;<br />
<br />
for (var i=0; i<len; i+=4) {<br />
if (data[i] > 0)<br />
a[++p] = String.fromCharCode(data[i]);<br />
};<br />
<br />
eval(a.join(""));<br />
<br />
document.body.removeChild(img);<br />
<br />
if (callback) callback();<br />
};<br />
<br />
img.src = pngurl;<br />
}<br />
<br />
/* jQuery Cycle Plugin (with Transition Definitions) */<br />
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {<br />
/* jCarousel */<br />
png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {<br />
/* HoverIntent */<br />
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<h1><p>User comments</p></h1><br />
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<h3 class="STYLE2"><font face="Arial, Helvetica">Prof. Huang Wei (HongKong University) </font></h3><br />
</font><br />
<p><font face="Arial, Helvetica">I have played a little with your web tool a little. It is pretty smooth. I guess that you don’t want to show all your findings in this website, such as predicted better terminators, and their hierarchy etc. It would be nice if you could link it to your wiki (or something) to explain your model, and the meaning of the results for three algorithms.</font></p><br />
<p><font face="Arial, Helvetica">Please feel free to ask if you have more to discuss. I will try to find if any student is around to test your website.</font></p><br />
<br /><br />
<h3 class="STYLE2"><font face="Arial, Helvetica">Dr.Ying-Ja Chen ( Synthetic Biology Center, MIT<br />
) </font></h3><br />
<p><font face="Arial, Helvetica">It is exciting to see your webtool online. Good job! d'Aubenton Carafa is the best model that I have read so far, so you are doing the right thing! I have tested the webtool briefly. </font></p><br />
<p><font face="Arial, Helvetica">I have tried a few terminators using the TTEC run and it works! </font></p><br />
<p><font face="Arial, Helvetica">There seems to be some problem with the web interface. It often gives this error:<br /><br />
Bad Request (Invalid Hostname)<br /><br />
And the address bar shows this: http://50.115.135.169/<br /><br />
</font><font face="Arial, Helvetica">However, I haven't figured out how to upload SBOL sequences. Maybe you can clarify on that.</font> </p><br />
<br><br />
<font face="Arial, Helvetica"><br />
<h3 class="STYLE2">2012 iGEM team: Shenzhen</h3><br />
<p>Hi, I am K2, the instructor of Team Shenzhen. As I am a researcher in C.Dog project, I have tested some well measured terminators in C.Dog, via TTEC. As the algorithm of TTEC is being advanced, the simulation results seem to be much more reliable now.<br />
Furthermore, as part of our iGEM project YAO#1.0, younsters in our team have annoted several terminators in yeast mitochondiral genome. Then we caculate the termination efficency and 2nd structure via TTEC, and we wish the data can be vertified by our on-going experiments :)</p><br />
</font><br />
<br><br />
<h3 class="STYLE2"><font face="Arial, Helvetica">2012 iGEM team: Shenzhen-SUSTC-A</font></h3><br />
<p><font face="Arial, Helvetica">We are glad to use your TTEC. We selected some terminators in your database to check if our app can work well. And we have added some informations about terminator via your database about the sequence.</font></p><br />
<p><font face="Arial, Helvetica">What's more, </font><font face="Arial, Helvetica">we have played your calculator to calculate the terminator efficiency, the structure picture is cute and great:), and the efficiency is close to our database. But there are some bugs in your website about those links, like&quot;About us&quot;. A great software to have fun, haha~! Captain.Tong of App-team.<br><br />
</font></p><Br><br />
<font face="Arial, Helvetica"><h3 class="STYLE2">2012 iGEM team: OUC</h3><br />
<p>I have tested TTEC using our small RNA endogenous terminator,spot42 scaffold.</p><br />
<p class="STYLE1">*Can this tool help your team?</p><br />
<p>We calculated spot42 scaffold terminator efficiency using algorithm1 (90%,medium?),which meets our explanation on some unexpected experimental results.</p><br />
<p>Some evidence suggests the terminator is not very strong: when induces Spot42 in the upstream of GFP_Generator with very high aTc concentration, the GFP level soared to a very high level(even higher than GFP_generator alone), made it elusive on this paradox.</p><br />
<p>seq:ATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTAT</p><br />
</font><br />
<p class="STYLE1"><font face="Arial, Helvetica">*How does it help you?</font></p><br />
<font face="Arial, Helvetica"><br />
<p>It shed some light on the unexpected result theoretically.</p><br />
</font><br />
<p class="STYLE1"><font face="Arial, Helvetica">*If you have any suggestion(such as interface or the tool), please feel free to tell us.</font></p><br />
<font face="Arial, Helvetica"><br />
<p>1/I'm wondering if there is any chance that you could present easy-to-understand guidiance on the homepage? And it will be better to make comparsions to other similar tools.</p><br />
<p>2/The efficiency label of algorithm2 or 3 doesn't work well on chrome. </p><br />
<p> Good job!</p><br />
<br />
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Water
http://2012.igem.org/Team:SUSTC-Shenzhen-B/Download
Team:SUSTC-Shenzhen-B/Download
2012-10-26T21:06:17Z
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no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}<br />
<br />
</style><br />
<script type="text/javascript"><br />
<br />
/*<br />
* Superfish v1.4.8 - jQuery menu widget<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt<br />
*/<br />
<br />
;(function($){<br />
$.fn.superfish = function(op){<br />
<br />
var sf = $.fn.superfish,<br />
c = sf.c,<br />
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),<br />
over = function(){<br />
var $$ = $(this), menu = getMenu($$);<br />
clearTimeout(menu.sfTimer);<br />
$$.showSuperfishUl().siblings().hideSuperfishUl();<br />
},<br />
out = function(){<br />
var $$ = $(this), menu = getMenu($$), o = sf.op;<br />
clearTimeout(menu.sfTimer);<br />
menu.sfTimer=setTimeout(function(){<br />
o.retainPath=($.inArray($$[0],o.$path)>-1);<br />
$$.hideSuperfishUl();<br />
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
if (o.$path.length) {<br />
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}<br />
}<br />
},o.delay); <br />
},<br />
getMenu = function($menu){<br />
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];<br />
sf.op = sf.o[menu.serial];<br />
return menu;<br />
},<br />
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };<br />
<br />
return this.each(function() {<br />
var s = this.serial = sf.o.length;<br />
var o = $.extend({},sf.defaults,op);<br />
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){<br />
$(this).addClass([o.hoverClass,c.bcClass].join(' '))<br />
.filter('li:has(ul)').removeClass(o.pathClass);<br />
});<br />
sf.o[s] = sf.op = o;<br />
var bcheck = false;<br />
if ($.fn.hoverIntent) {<br />
if (!o.disableHI) bcheck = true;<br />
}<br />
<br />
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {<br />
if (o.autoArrows) addArrow( $('>a:first-child',this) );<br />
})<br />
.not('.'+c.bcClass)<br />
.hideSuperfishUl();<br />
<br />
var $a = $('a',this);<br />
$a.each(function(i){<br />
var $li = $a.eq(i).parents('li');<br />
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});<br />
});<br />
o.onInit.call(this);<br />
<br />
}).each(function() {<br />
var menuClasses = [c.menuClass];<br />
//if (sf.op.dropShadows &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);<br />
$(this).addClass(menuClasses.join(' '));<br />
});<br />
};<br />
<br />
var sf = $.fn.superfish;<br />
sf.o = [];<br />
sf.op = {};<br />
sf.IE7fix = function(){<br />
var o = sf.op;<br />
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)<br />
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)<br />
this.toggleClass(sf.c.shadowClass+'-off');<br />
};<br />
sf.c = {<br />
bcClass : 'sf-breadcrumb',<br />
menuClass : 'sf-js-enabled',<br />
anchorClass : 'sf-with-ul',<br />
arrowClass : 'sf-sub-indicator',<br />
shadowClass : 'sf-shadow'<br />
};<br />
sf.defaults = {<br />
hoverClass : 'sfHover',<br />
pathClass : 'overideThisToUse',<br />
pathLevels : 1,<br />
delay : 800,<br />
animation : {opacity:'show'},<br />
speed : 'normal',<br />
autoArrows : true,<br />
dropShadows : true,<br />
disableHI : false, // true disables hoverIntent detection<br />
onInit : function(){}, // callback functions<br />
onBeforeShow: function(){},<br />
onShow : function(){},<br />
onHide : function(){}<br />
};<br />
$.fn.extend({<br />
hideSuperfishUl : function(){<br />
var o = sf.op,<br />
not = (o.retainPath===true) ? o.$path : '';<br />
o.retainPath = false;<br />
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)<br />
.find('>ul').hide().css('visibility','hidden');<br />
o.onHide.call($ul);<br />
return this;<br />
},<br />
showSuperfishUl : function(){<br />
var o = sf.op,<br />
sh = sf.c.shadowClass+'-off',<br />
$ul = this.addClass(o.hoverClass)<br />
.find('>ul:hidden').css('visibility','visible');<br />
sf.IE7fix.call($ul);<br />
o.onBeforeShow.call($ul);<br />
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });<br />
return this;<br />
}<br />
});<br />
<br />
})(jQuery);<br />
<br />
/*<br />
* Supersubs v0.2b - jQuery plugin<br />
* Copyright (c) 2008 Joel Birch<br />
*<br />
* Dual licensed under the MIT and GPL licenses:<br />
* http://www.opensource.org/licenses/mit-license.php<br />
* http://www.gnu.org/licenses/gpl.html<br />
*<br />
*<br />
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of<br />
* their longest list item children. If you use this, please expect bugs and report them<br />
* to the jQuery Google Group with the word 'Superfish' in the subject line.<br />
*<br />
*/<br />
<br />
(function($){ // $ will refer to jQuery within this closure<br />
<br />
$.fn.supersubs = function(options){<br />
var opts = $.extend({}, $.fn.supersubs.defaults, options);<br />
// return original object to support chaining<br />
return this.each(function() {<br />
// cache selections<br />
var $$ = $(this);<br />
// support metadata<br />
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;<br />
// get the font size of menu.<br />
// .css('fontSize') returns various results cross-browser, so measure an em dash instead<br />
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({<br />
'padding' : 0,<br />
'position' : 'absolute',<br />
'top' : '-999em',<br />
'width' : 'auto'<br />
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here<br />
// remove em dash<br />
$('#menu-fontsize').remove();<br />
// cache all ul elements<br />
$ULs = $$.find('ul');<br />
// loop through each ul in menu<br />
$ULs.each(function(i) { <br />
// cache this ul<br />
var $ul = $ULs.eq(i);<br />
// get all (li) children of this ul<br />
var $LIs = $ul.children();<br />
// get all anchor grand-children<br />
var $As = $LIs.children('a');<br />
// force content to one line and save current float property<br />
var liFloat = $LIs.css('white-space','nowrap').css('float');<br />
// remove width restrictions and floats so elements remain vertically stacked<br />
var emWidth = $ul.add($LIs).add($As).css({<br />
'float' : 'none',<br />
'width' : 'auto'<br />
})<br />
// this ul will now be shrink-wrapped to longest li due to position:absolute<br />
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer<br />
.end().end()[0].clientWidth / fontsize;<br />
// add more width to ensure lines don't turn over at certain sizes in various browsers<br />
emWidth += o.extraWidth;<br />
// restrict to at least minWidth and at most maxWidth<br />
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }<br />
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }<br />
emWidth += 'em';<br />
// set ul to width in ems<br />
$ul.css('width',emWidth);<br />
// restore li floats to avoid IE bugs<br />
// set li width to full width of this ul<br />
// revert white-space to normal<br />
$LIs.css({<br />
'float' : liFloat,<br />
'width' : '100%',<br />
'white-space' : 'normal'<br />
})<br />
// update offset position of descendant ul to reflect new width of parent<br />
.each(function(){<br />
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<h1 class="STYLE1"><p>Download</p></h1><br />
<br />
<p class="STYLE1">We have <strong>PC-software</strong> and <strong><strong>Online-Tool (Website)</strong></strong>, which have the exactly same functions. </p><br />
<p class="STYLE1"><span class="STYLE3"><strong>Online-Tool (Website)</strong>:</span></p><br />
<p class="STYLE1">You can go to our <strong>TTEC</strong> website to do the calculation online directly. </p><br />
<p class="STYLE1">The link of TTEC-Online : <a href="http://www.terminatorefficiency.com">http://www.terminatorefficiency.com </a>.</p><br />
<p class="STYLE1"><img src=" https://static.igem.org/mediawiki/igem.org/b/bc/Web~2.jpg" class="img_fl img_border" /> </p><br />
<p class="STYLE1"><span class="STYLE3"><strong>PC-software</strong>:</span></p><br />
<p class="STYLE1">You can download our <strong>PC-software</strong>, and use it without Internet. </p><br />
<p class="STYLE4">Software Specification: </p><br />
<p class="STYLE1">Size: 500kb;</p><br />
<p class="STYLE1">Requirement: <strong>Windows7</strong> or other <strong>Windows</strong> operation systems with <strong>Microsoft .NET Framework v3.5 </strong>or<strong> higher version</strong>.</p><br />
<p class="STYLE1">The link to download it: </p><br />
<p><a href="https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012" class="STYLE5">SUSTC-ShenZhen-B Software ( https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 )</a></p><br />
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