http://2012.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=250&target=PhilippBoeing&year=&month=2012.igem.org - User contributions [en]2024-03-29T15:10:07ZFrom 2012.igem.orgMediaWiki 1.16.0http://2012.igem.org/IGEM_PublicityIGEM Publicity2012-12-07T20:52:22Z<p>PhilippBoeing: /* team specific */</p>
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<div>__NOTOC__<br />
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<div style="color:#aaa; padding-bottom:20px;">(Members of the press, please see the [https://igem.org/Press_Kit | iGEM Press Kit])</div><br />
<br />
If you would like to share an article that was written about iGEM or your iGEM team, please link to it on this page. If you have multiple articles featuring your team, link to them all individually!<br />
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Post your official team name, the title of your article, where it was featured, and provide a link to it. <br />
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''Example'':<br> <br />
'''Team iGEM Headquarters''': ''Title of article'', Nature, [link]<br />
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<span style="color:#1e90ff; font-size:150%">'''blogs </span><span style="color:#3cb371; font-size:150%">covering iGEM 2012'''</span><br><br />
'''Peking2012 iGEM''': ''Peking iGEM'', [http://page.renren.com/601444914] <br><br />
'''UANL Mexico''': ''Bio! iGEM Collection'', Blogspot, [http://biospot10.blogspot.de/2012/02/bio-about-igem.html]<br><br />
'''iGEM Valencia 2012''': ''Synth(ethic) Biology'', Wordpress, [http://igemvalencia2012.wordpress.com/]<br><br />
'''TU_Munich''': ''TUM iGEM 2012'', [http://tum-igem2012.blog.de/]<br><br />
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<span style="color:#1e90ff; font-size:150%">'''video/radio</span><span style="color:#3cb371; font-size:150%"> about iGEM 2012'''</span><br><br />
'''TU_Munich''': LIVE radio interview, Kortex: TUM-Projektteam iGEM 2012 - Bier und synthetische Biologie, M94.5, soundcloud, [http://soundcloud.com/m945/kortex-tum-projektteam-igem]<br />
<br><br />
'''TU_Munich''': ''TUM Brew'', [http://vimeo.com/51804324]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices ---"Sowing Tomorrow Synthetic Biologists"'', [http://youtu.be/cd7m5POAwRY] <br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- Intro to Synthetic Biology'', [http://youtu.be/BpmCMeBqZuI]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- Research guideline of Synthetic Biology'', [http://youtu.be/gfNFW6LQ1SQ]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- iGEM HS-Division in a Nutshell'', [http://youtu.be/R3g0zVi0DgI]<br />
<br><br />
'''University College London''': ''#gemFM'', radioshow Mixcloud / Soundcloud, [https://2012.igem.org/Team:University_College_London/gemFM] <br><br />
'''Bielefeld-Germany''': ''LIVE radio interview'', Radio Hertz, [http://www.radiohertz.de/beta-site/2012/07/02/impuls-am-04-juli-2012/]<br><br />
'''SDU-Denmark''': ''Forsker i fritiden'', TV2 Fyn, [http://www.tv2fyn.dk/article/370281?autoplay=1&video_id=54463]<br><br />
'''University College London''':''London 'bio-hackers' catching the eye of professionals'', BBC News TV, [http://www.bbc.co.uk/news/uk-england-london-19964886]<br /><br />
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<span style="color:#1e90ff; font-size:150%">'''news articles</span><span style="color:#3cb371; font-size:150%"> about iGEM 2012'''</span><br />
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'''Peking2012 iGEM''': ''Lecture "Life as Machines --- Rational Design for Artificial Biological Systems"'', Beijing Teenager Science and Technology Club, [http://www.scitech-youth.org.cn/club_news/dt/4676.html] <br><br />
'''iGEM Leicester''', ''Google Campus hosts future gene-iuses'', University of Leicester press office, [http://www2.le.ac.uk/news/blog/2012/august/google-campus-hosts-future-gene-iuses]<br><br />
'''Lyon-INSA iGEM''': ''Ready, Set, Go for OSLI 2012 iGEM Teams'', Osli, [http://www.osli.ca/storybank/39/59/Ready-Set-Go-for-OSLI-2012-iGEM-Teams]<br />
'''TU_Munich''': ''TUM Brew'', [http://vimeo.com/51804324]<br><br />
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====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
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'''Peking2012 iGEM''': ''Designing the Scientific Cradle for Quantitative Biologists'', ACS Synth. Biol., [http://pubs.acs.org/doi/abs/10.1021/sb3000386]<br />
<br><br />
'''iGEM Teams Germany''', ''iGEM: Elf Teams tüfteln für die Biokonstrukteurs-WM'', biotechnologie.de, [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153792.html]<br><br />
'''iGEM Teams France''', ''Site iGEM France'', [https://sites.google.com/site/igemfrance/]<br><br />
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'''Buenos Aires 2012 iGEM Team''', Synthetic Communities, <br />
[http://blogs.scientificamerican.com/lab-rat/2012/09/09/igem-buenos-aires-synthetic-bacterial-communities/]<br><br />
'''SDU-Denmark''': ''iGEM – Verdens største konkurrence indenfor syntetisk biologi vender tilbage til SDU'', Sund & Hed, [http://sundoghed.dk/7038/igem-verdens-storste-konkurrence-indenfor-syntetisk-biologi-vender-tilbage-til-sdu/?fb_comment_id=fbc_365648506787388_4738817_366895283329377]<br><br />
<br />
'''Waterloo 2012 iGEM Team''', Educational Podcast on SynBio by Virtual Researcher On Call (Canadian Educational Resource Organization) [http://sns.vroc.ca/p.jsp?i=17]<br><br />
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====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
'''Arizona_State''': ''Students create low-cost biosensor to detect contaminated water in developing nations'',[https://asunews.asu.edu/20120906_waterbiosensor]<br/><br />
'''Potsdam-Bioware''': ''Hamsterzellen als Antikörperfabrik, biotechnologie.de'',[http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153816.html]<br/><br />
'''Valencia (UPV,UCV)''' : ''Seminar at the Institute of marine science (CSIC, Barcelona)'',[http://igemvalencia2012.wordpress.com/2012/09/11/seminar-in-barcelona/] <br/><br />
'''Valencia (UPV,UCV)''' : ''Interview with D. Justo Aznar, an expert in Bioethics, Wordpress'',[http://igemvalencia2012.wordpress.com/2012/08/29/interview-with-justo-aznar-lucena-m-d/] <br/><br />
'''Valencia (UPV,UCV)''' : ''What do you know about Synthetic Biology?, Wordpress'',[http://igemvalencia2012.wordpress.com/2012/09/07/what-do-you-know-about-synthetic-biology/] <br/><br />
'''TU Delft''' : ''Our team gets featured in the national news paper'',[https://static.igem.org/mediawiki/igem.org/c/c7/ZomerrepoNRC7aug.pdf] <br/><br />
'''TU Delft''' : '' Team members reaching out to the general public at The Night of Art and Science in Groningen to spread awareness on Synthetic Biology '', [http://www.youtube.com/watch?v=j9t7-Qnho7o&feature=share] <br/><br />
'''TU Delft''' : ''Licht aan de bacterie - Our team member Sietske explains how cool and fascinating Synthetic Biology is for LowLabs!! '', [http://www.youtube.com/watch?v=4q2Ol89ekz4&feature=youtu.be] <br/><br />
'''Valencia (UPV,UCV)''': ''Biohacking: Do it yourself!'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/21/biohacking-do-it-yourself/] <br/><br />
'''Valencia (UPV,UCV)''': ''Where will synthetic biology lead us?'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/13/a-life-of-its-own-where-will-synthetic-biology-lead-us-by-michael-specter/] <br/><br />
'''Valencia (UPV,UCV)''': ''iGEM: Why of all this'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/14/igem-the-why-of-all-this/] <br/><br />
'''University of Leicester''': ''Synthetic biology solution to polystyrene degradation'', Blogger, [http://uoleicesterigem2012.blogspot.co.uk/] <br/><br />
'''University College London''': ''Land Grab: Could Bioremediation Turn Pacific Garbage Patch Into Habitable Island?'', Good, [http://www.good.is/post/land-grab-could-bioremediation-turn-pacific-garbage-patch-into-habitable-island/] <br/><br />
'''University College London''': ''Synthetic Bacteria Could Turn Ocean Garbage into One Big Island'', Smithsonian, [http://blogs.smithsonianmag.com/smartnews/2012/07/synthetic-bacteria-could-turn-ocean-garbage-into-one-big-island/] <br/><br />
'''University College London''': ''Students synthesizing bacteria to create islands out of garbage'', DVICE, [http://dvice.com/archives/2012/07/students-synthe.php] <br/><br />
'''University College London''': ''Nanobots could turn 'Great Pacific Plastic Patch' into a floating island'', Wired, [http://www.wired.co.uk/news/archive/2012-07/18/nanobots-recycling-plastic] <br/><br />
'''University College London''': ''Synthetic Biology Speed Debate'', C-LAB, [http://c-lab.co.uk/events/synthetic-biology-speed-debate.html] <br/><br />
'''University College London''': ''From Pixels To DNA: The Next Frontier In Interaction Design'', Co.Design, [http://www.fastcodesign.com/1670825/from-pixels-to-dna-the-next-frontier-in-interaction-design#1]<br /><br />
'''University College London''': ''Biohacking, iGEM and the limits of citizen science'', Po Ve Sham, [http://povesham.wordpress.com/2012/10/05/biohacking-igem-and-the-limits-of-citizen-science/]<br /><br />
'''University College London''': ''Right or Risk? World's First Public BioBrick'', C-LAB, [http://c-lab.co.uk/events/right-or-risk-worlds-first-public-biobrick.html]<br /><br />
'''University College London''': ''Biohackers on the Rise'', British Science Association People & Science, [http://www.britishscienceassociation.org/people-science-magazine/december-2012/biohackers-rise]<br/><br />
'''Queen's University''': ''Getting Biological over the Break'', Kingston This Week, [http://www.kingstonthisweek.com/2012/06/21/getting-biological-over-the-break] <br/><br />
'''Queen's University''': ''Dance like everybody's watching'', Kingston EMC, [http://www.emckingston.com/20120809/lifestyle/Dance+like+everybody%27s+watching] <br/><br />
'''Queen's University''': ''Dancing in the Lab'', The Queen's Journal, [http://queensjournal.ca/story/2012-09-11/news/dancing-lab/] <br/><br />
'''NRP UEA Norwich''': ''UEA Students Compete For Top Biology Prize'', UEA Press Release, [http://www.uea.ac.uk/mac/comm/media/press/2012/August/igem-competition-uea]<br><br />
'''NRP UEA Norwich''': ''Interview With Students From The UEA Talking About Synthetic Biology (Show 23)'', The Farming Show: STAR Radio, [http://www.star107.co.uk/farming-show.php]<br><br />
'''University of St Andrews''': "Streets of London: paved with platinum" [http://www.st-andrews.ac.uk/news/archive/2012/Title,89409,en.html] <br><br />
'''TU_Munich''': ''Technische Universität München: Brauhefe für leckeres und gesundes Bier'', biotechnologie.de, [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153818.html]<br><br />
'''TU_Munich''': ''TUMstudis: baker's yeast is synthetically converted'', TUMstudinews, [http://portal.mytum.de/ccc/newsletter/students/2012_03/02]<br><br />
'''TU_Munich''': ''Die Wunderhefe'', jetzt.de sueddeutsche zeitung, [http://jetzt.sueddeutsche.de/texte/anzeigen/544866/4/1#texttitel]<br><br />
'''TU_Munich''': ''Widerstand gegen die "Grüne Gentechnik" wächst weiter'', merkur, [http://www.merkur-online.de/lokales/freising/widerstand-gegen-gruene-gentechnik-waechst-weiter-2510648.html]<br><br />
'''TU_Munich''': ''iGEM: Fünf Teams lösen Ticket für Boston'', biotechnologie.de, [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=154774.html]<br><br />
'''Tübingen''': ''Tübinger Studenten nehmen an internationalem Forscherwettbewerb teil'', Schwäbisches Tagblatt, [http://www.tagblatt.de/Home/nachrichten/hochschule_artikel,-Tuebinger-Studenten-nehmen-an-internationalem-Forscherwettbewerb-teil-_arid,184906.html]<br><br />
'''Bielefeld-Germany''': ''Studierende der Universität Bielefeld nehmen am iGEM-Wettbewerb teil'', Universität Bielefeld, [http://ekvv.uni-bielefeld.de/blog/pressemitteilungen/entry/östrogen_aus_trinkwasser_entfernen_nr]<br><br />
'''Bielefeld-Germany''': ''Östrogen aus Trinkwasser entfernen'', Press Release, [http://www.uni-protokolle.de/nachrichten/id/240229/][http://www.laborpraxis.vogel.de/forschung-und-entwicklung/analytik/articles/369125/][http://www.bio.nrw.de/nachrichten][http://idw-online.de/pages/de/news484982][http://www.eurekalert.org/pub_releases/2012-06/uob-ref062512.php]<br><br />
'''Bielefeld-Germany''': ''Turkey tail tree fungus could filter oestrogen from water'', Wired, [http://www.wired.co.uk/news/archive/2012-06/25/tree-fungus-oestrogen-filter]<br><br />
'''Bielefeld-Germany''': ''Biological filter to remove estrogens from waste water and drinking water'', News Medical Australia, [http://www.news-medical.net/news/20120626/Biological-filter-to-remove-estrogens-from-waste-water-and-drinking-water.aspx]<br><br />
'''Bielefeld-Germany''': ''Das Wasser wird weiblich'', Neue Westfälische, [https://2012.igem.org/Team:Bielefeld-Germany/Homeleft]<br><br />
'''Bielefeld-Germany''': ''Arzneimittel in Gewässern minimieren'', Neue Züricher Zeitung, [http://www.nzz.ch/wissen/wissenschaft/arzneimittel-in-gewaessern-minimieren-1.17368069]<br><br />
'''USP-UNESP-Brazil''': ''Brazilian Science Dreams Come Alive'', RocketHub Blog, [http://blog.rockethub.com/brazilian-science-dreams-come-alive]<br><br />
'''USP-UNESP-Brazil''': ''USP participa pela 1ª vez de campeonato de biologia sintética'', Jornal do Campus, [http://www.jornaldocampus.usp.br/index.php/2012/06/usp-participa-pela-1a-vez-de-campeonato-de-biologia-sintetica/]<br><br />
'''USP-UNESP-Brazil''': ''Cientistas da USP conseguem financiar projeto com vaquinha virtual'', Folha de S.Paulo, [http://www1.folha.uol.com.br/ciencia/1097202-cientistas-da-usp-conseguem-financiar-projeto-com-vaquinha-virtual.shtml]<br><br />
'''EPF-Lausanne''': ''Fabriquer des médicaments en allumant la lumière?'' (French), Flash EPFL, [http://actualites.epfl.ch/newspaper-edition?np_id=151] [https://static.igem.org/mediawiki/2012/4/42/Team-EPF-Lausanne_Flash_article_about_us.pdf]<br><br />
'''Lyon INSA iGEM''' : ''L’INSA de Lyon vise l’or à Amsterdam'' , En vue INSA Lyon, [http://envue.insa-lyon.fr/2012avril/art2a_0412.php]<br><br />
'''Trieste''': ''Human practices - SynBio for everyone (Italian)'', Il Tascapane, [http://tascapane.blogspot.se/2012/08/la-biologia-sintetica.html]<br><br />
'''Trieste''': ''Human practices - SynBio and medicine (Italian)'', Il Tascapane,[http://tascapane.blogspot.it/2012/09/la-biologia-sintetica-approda-in.html]<br><br />
'''Trieste''': '' Un'idea per proteggere l'intestino (Italian)'', ICGEB, [http://www.icgeb.org/notizie-in-italiano/items/squadra-igem-2012-un-idee-per-proteggere-lintestino.html]<br><br />
'''Lyon-INSA''':''M. concourt au titre mondial de biologie de synthèse'', Républicain Lorrain [https://docs.google.com/file/d/0BzhWvySCD_KzWmJTYjBYWHBCUnM/edit]<br><br />
'''Lyon-INSA''' : ''L'Insa de Lyon vise l'or à Amsterdam'', Insa de Lyon [http://www.insa-lyon.fr/fr/concours-igem-2012]<br><br />
'''UANL_Mty-Mexico''': ''Túnel de la Biología Sintética (Synthetic Biology Tunnel), Televisa'', Monterrey,[http://www.televisaregional.com/monterrey/video/169491536.html]<br/><br />
'''Wageningen_UR''': ''The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts'', Journal of Biological Engineering,[http://www.jbioleng.org/content/6/1/14/abstract]<br/><br />
'''SDU-Denmark''': ''Fighting Obesity at the SDU in Odense'', SIJ, by Eliana van de Craats, [http://community.ejc.net/forum/topic/show?id=2345725%3ATopic%3A89026&xgs=1&xg_source=msg_share_topic]<br/><br />
'''Georgia_Tech''': ''<br />
Georgia Tech workshop well received: Lambert students embrace engineering'', ForsythNews.com, [http://www.forsythnews.com/m/section/3/article/14721/. ]<br/><br />
'''Cornell''': ''Using electroactive bacteria, students design toxin sensor'', Cornell Chronicle, [http://www.news.cornell.edu/stories/Oct12/oilSands.html]</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/ModellingTeam:University College London/Modelling2012-10-26T23:17:59Z<p>PhilippBoeing: </p>
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<div>{{:Team:University_College_London/templates/headnocover}}<html><img src="http://www.plasticrepublic.org/wikifiles/overview-modelling.jpg" /></html><br />
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{{:Team:University_College_London/templates/modellingmenu}}<br />
== Our Models ==<br />
The [https://2012.igem.org/Team:University_College_London/Modelling/OceanModel ocean model] and [https://2012.igem.org/Team:University_College_London/Modelling/DensityPredictions density predictions] shown here aim to predict how our system as a whole might function in the ocean. The 'bigger picture' presented by these models will guide us in fine-tuning each individual module. However, the bulk of our modelling looks at the performance of each module as a separate unit. For these models, please see the 'Modelling' tab on the individual module pages, which starts [[Team:University_College_London/Module_1/Modelling| here]] with our cell model for detection. <br />
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<br />
Quick links to the models:<br />
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[https://2012.igem.org/Team:University_College_London/Module_1/Modelling 1. Detection]<br />
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[https://2012.igem.org/Team:University_College_London/Module_2/Modelling 2. Aggregation]<br />
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[https://2012.igem.org/Team:University_College_London/Module_3/Modelling 3. Degradation]<br />
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[https://2012.igem.org/Team:University_College_London/Module_4/Modelling 4. Buoyancy]<br />
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[https://2012.igem.org/Team:University_College_London/Module_5/Modelling 5. Salt Tolerance]<br />
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[https://2012.igem.org/Team:University_College_London/Module_6/Modelling 6. Containment]<br />
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{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/AchievementsTeam:University College London/Achievements2012-10-26T23:17:34Z<p>PhilippBoeing: </p>
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<div>{{:Team:University_College_London/templates/headnocover}}<html><img src="http://www.plasticrepublic.org/wikifiles/overview-achievements.jpg" /></html><br />
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== Meeting of Young Minds Debate Proposal ==<br />
This October, the Rathenau Instituut (in collaboration with the African-European iGEM organization committee) will stage the Meeting of Young Minds Debate 2012, in which young people representing the worlds of science, the public and policy making will come together to debate around the topic of synthetic biology and society. Our proposal (‘A synthetic biology future, a synthetic biology crisis’) is one of two themes selected for debate. For more on the Meeting of Young Minds debate, see [[Team:University_College_London/HumanPractice/MOYM | here]].<br />
<br />
== Collaboration with iGEM Bielefeld ==<br />
<br />
This year we had an opportunity to collaborate with the iGEM Bielefeld team which is also working with laccase enzymes, although Bielefeld is working on laccase from several species. iGEM Bielefeld kindly sent their laccase from ''E. coli'' strain BL21 (DE3). As we are working on laccase from W3110, we welcomed the opportunity to compare our enzyme activities using the same protocol. Bielefeld team’s laccase seem to have higher activity in comparison to ours. This is due to the fact that our laccase is periplasmic, whilst theirs is extracellular, therefore more of it can be found in the supernatant after centrifugation. For more details about the assay used to determine laccase activity and the results obtained, please see the following link: http://partsregistry.org/Part:BBa_K863005:Experience<br />
<br />
We believe that collaborations between iGEM teams should be encouraged. Firstly, this strengthens the reliability of the registry, since the more evaluations and characterisation the BioBrick has, the more reliable and applicable it is. Secondly, such collaborations allow for an exchange of ideas, giving us the insight and opportunity to learn from alternative culturing and characterisation techniques of other groups. Finally, this collaboration has allowed us to build links between the two universities.<br />
<br />
== German Modelling Collaborations ==<br />
'''LMU Munich: Modelling Workshop'''<br /><br />
LMU Munich invited us to the CAS Conference on Synthetic Biology at their beautiful campus outside of Munich city centre. We met up with the team after the conference had ended and spent a morning going through their project and looking at areas where modelling would be most helpful to their project. Ideas we came up with included using software e.g. COBRA to model the effect of gene knockouts on their systems, and using software called FPMOD to look at possible rotations of GFP. We also gave a short presentation to act as an introduction to biological modelling, covering MATLAB, ODEs, Kappa and CellDesigner.<br />
<br />
'''TU Darmstadt: Ocean Model'''<br /><br />
We chatted with their modeller Henrick about how we could improve our ocean model, as he is working on similar models related to fluid motion and dynamics. He proposed a couple of different approaches and suggested some software, YASARA, that would be helpful to us.<br />
<br />
== Human Practice Collaboration with iGEM Paris Bettencourt ==<br />
{{:Team:University_College_London/fbphotosbytudelft|album=357216511029407|count=6}}<br />
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We had the opportunity to visit Paris where we kindly hosted by Claire Mayer from iGEM Paris Bettencourt team. Philipp was invited as a judge for their <html><a href="https://2012.igem.org/Team:Paris_Bettencourt/Human_Practice/Debate" "title"=debate" target="_blank">debate on the environmental release of genetically modified bacteria</a></html>. During our two days in Paris, we attended a DIY class for the CRI undergraduate students, which we found very pertinent to our collaboration with the London Hackspace. We also had the chance to brainstorm with the iGEM team a number of potential future projects for our partnership with the Hackspace - one of our favourites was the idea of evolving bacterial art.<br />
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== Plastic Republic Speed Debate ==<br />
<br />
In August, we hosted an evening of speed debating to deliberate the question “Should synthetic organisms be released in the ocean to combat plastic pollution?”. We targeted the interest in our project to achieve a bottom-up collection of ideas to feed back into our work. The turn-out was very successful, with attendees ranging from all different backgrounds including Bloomberg, London Futurists group, London Hackspace and the Guardian.<br />
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== Fundraising Campaign ==<br />
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In July we ran a <html><a href="http://www.sponsume.com/project/plastic-republic" "title"=Sponsumewebsite" target="_blank">crowdfundraising campaign</a></html> on Sponsume. We successfully raised more than our target of £1,500! <br />
<br />
== Medal Requirements ==<br />
'''Bronze:'''<br />
<div class="achievement-tick">Team registration</div><br />
<div class="achievement-tick">Complete Judging form</div><br />
<div class="achievement-tick">Team Wiki</div><br />
<div class="achievement-tick">Present a poster and a talk at the iGEM Jamboree</div><br />
<div class="achievement-tick">At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts.</div><br />
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'''Silver:''' In addition to the Bronze Medal requirements...<br />
<div class="achievement-tick">Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected</div><br />
<div class="achievement-tick">Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry.</div><br />
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'''Gold:''' In addition to the Bronze and Silver Medal requirements, any one or more of the following:<br />
<div class="achievement-tick">Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year) and enter this information in the Registry (in the “Experience” section of that BioBrick’s Registry entry), and don't forget to create a new registry page for the improved part.<br /><br />
<br />
</div><br />
<div class="achievement-tick">Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.</div><br />
<div class="achievement-tick">Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.</div><br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/AchievementsTeam:University College London/Achievements2012-10-26T23:17:14Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headnocover}}<html><img src="http://www.plasticrepublic.org/wikifiles/overview-achievements.jpg" /></html><br />
<br />
= Achievements =<br />
<br />
== Meeting of Young Minds Debate Proposal ==<br />
This October, the Rathenau Instituut (in collaboration with the African-European iGEM organization committee) will stage the Meeting of Young Minds Debate 2012, in which young people representing the worlds of science, the public and policy making will come together to debate around the topic of synthetic biology and society. Our proposal (‘A synthetic biology future, a synthetic biology crisis’) is one of two themes selected for debate. For more on the Meeting of Young Minds debate, see [[Team:University_College_London/HumanPractice/MOYM | here]].<br />
<br />
== Collaboration with iGEM Bielefeld ==<br />
<br />
This year we had an opportunity to collaborate with the iGEM Bielefeld team which is also working with laccase enzymes, although Bielefeld is working on laccase from several species. iGEM Bielefeld kindly sent their laccase from ''E. coli'' strain BL21 (DE3). As we are working on laccase from W3110, we welcomed the opportunity to compare our enzyme activities using the same protocol. Bielefeld team’s laccase seem to have higher activity in comparison to ours. This is due to the fact that our laccase is periplasmic, whilst theirs is extracellular, therefore more of it can be found in the supernatant after centrifugation. For more details about the assay used to determine laccase activity and the results obtained, please see the following link: http://partsregistry.org/Part:BBa_K863005:Experience<br />
<br />
We believe that collaborations between iGEM teams should be encouraged. Firstly, this strengthens the reliability of the registry, since the more evaluations and characterisation the BioBrick has, the more reliable and applicable it is. Secondly, such collaborations allow for an exchange of ideas, giving us the insight and opportunity to learn from alternative culturing and characterisation techniques of other groups. Finally, this collaboration has allowed us to build links between the two universities.<br />
<br />
== German Modelling Collaborations ==<br />
'''LMU Munich: Modelling Workshop'''<br /><br />
LMU Munich invited us to the CAS Conference on Synthetic Biology at their beautiful campus outside of Munich city centre. We met up with the team after the conference had ended and spent a morning going through their project and looking at areas where modelling would be most helpful to their project. Ideas we came up with included using software e.g. COBRA to model the effect of gene knockouts on their systems, and using software called FPMOD to look at possible rotations of GFP. We also gave a short presentation to act as an introduction to biological modelling, covering MATLAB, ODEs, Kappa and CellDesigner.<br />
<br />
'''TU Darmstadt: Ocean Model'''<br /><br />
We chatted with their modeller Henrick about how we could improve our ocean model, as he is working on similar models related to fluid motion and dynamics. He proposed a couple of different approaches and suggested some software, YASARA, that would be helpful to us.<br />
<br />
== Human Practice Collaboration with iGEM Paris Bettencourt ==<br />
{{:Team:University_College_London/fbphotosbytudelft|album=357216511029407|count=6}}<br />
<br />
We had the opportunity to visit Paris where we kindly hosted by Claire Mayer from iGEM Paris Bettencourt team. Philipp was invited as a judge for their <html><a href="https://2012.igem.org/Team:Paris_Bettencourt/Human_Practice/Debate" "title"=debate" target="_blank">debate on the environmental release of genetically modified bacteria</a></html>. During our two days in Paris, we attended a DIY class for the CRI undergraduate students, which we found very pertinent to our collaboration with the London Hackspace. We also had the chance to brainstorm with the iGEM team a number of potential future projects for our partnership with the Hackspace - one of our favourites was the idea of evolving bacterial art.<br />
<br />
== Plastic Republic Speed Debate ==<br />
<br />
In August, we hosted an evening of speed debating to deliberate the question “Should synthetic organisms be released in the ocean to combat plastic pollution?”. We targeted the interest in our project to achieve a bottom-up collection of ideas to feed back into our work. The turn-out was very successful, with attendees ranging from all different backgrounds including Bloomberg, London Futurists group, London Hackspace and the Guardian.<br />
<br />
== Fundraising Campaign ==<br />
<br />
In July we ran a <html><a href="http://www.sponsume.com/project/plastic-republic" "title"=Sponsumewebsite" target="_blank">crowdfundraising campaign</a></html> on Sponsume. We successfully raised more than our target of £1,500! <br />
<br />
== Medal Requirements ==<br />
'''Bronze:'''<br />
<div class="achievement-tick">Team registration</div><br />
<div class="achievement-tick">Complete Judging form</div><br />
<div class="achievement-tick">Team Wiki</div><br />
<div class="achievement-tick">Present a poster and a talk at the iGEM Jamboree</div><br />
<div class="achievement-tick">At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts.</div><br />
<br />
'''Silver:''' In addition to the Bronze Medal requirements...<br />
<div class="achievement-tick">Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected</div><br />
<div class="achievement-tick">Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry.</div><br />
<br />
'''Gold:''' In addition to the Bronze and Silver Medal requirements, any one or more of the following:<br />
<div class="achievement-tick">Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year) and enter this information in the Registry (in the “Experience” section of that BioBrick’s Registry entry), and don't forget to create a new registry page for the improved part.<br /><br />
<br />
</div><br />
<div class="achievement-tick">Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.</div><br />
<div class="achievement-tick">Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.</div><br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/HumanPracticeTeam:University College London/HumanPractice2012-10-26T23:15:54Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headnocover}}<html><img src="http://www.plasticrepublic.org/wikifiles/overview-hp.jpg" /></html><br />
<br />
==Overview ==<br />
''Will the world be a safe place if we make biology easy to engineer? How do the lessons of the past inform the discussion going forward? Think beyond just convincing people that 'synthetic biology is good'.''<br />
<br />
Inspired by these quotes from the iGEM website, this year we set out to ‘challenge’ public perceptions about genetically modified organisms and test the boundaries of access to the tools of Synthetic Biology by helping the [[Team:University_College_London/HumanPractice/DIYbio|London Biohackers build their own BioBrick]] - the first "Public" BioBrick. We believe projects such as ours will only become realistic and widely accepted if access to the technology is increased.<br />
<br />
Plastic Republic raises a number of ethical, legal and social issues, which we have used as the basis for our [[Team:University_College_London/HumanPractice/MOYM|Meeting of the Young Minds debate proposal]], [[Team:University_College_London/HumanPractice/SpeedDebating|an exciting "speed-debating" evening]] and an investigation into the [[Team:University_College_London/HumanPractice/LegalBits|legal issues regarding our plastic island.]]<br />
<br />
We have also planned a [[Team:University_College_London/gemFM|radio show]], which will involve interviews with international teams giving people a sense of the global iGEM community and the breadth and diversity of synthetic biology projects. <br />
<br />
In addition, we are working with Carina Tran, an architectural student from the UCL Bartlett, faculty of the Built Environment who is particularly interested in how plastics can be recycled in terms of being used as a constructive material. We hope such collaboration will help us to develop a better project, [[Team:University_College_London/HumanPractice/LivingArchitecture|exploring iGEM in an architectural context.]] <br />
<br />
Last but not least, we raised funding for all these human practice activities via [[Team:University_College_London/HumanPractice/CrowdFunding|crowdfunding, and wrote a guide to successful crowdfunding campaigns for iGEM teams.]]<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/ResearchTeam:University College London/Research2012-10-26T23:15:20Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headnocover}}<html><img src="http://www.plasticrepublic.org/wikifiles/overview-research.png" /></html><br />
== Project Overview ==<br />
<br />
In many of the world's oceans, currents carry debris and pollution originating from coastlines. This waste accumulates in regional gyres - where ocean currents meet, and can reach extremely high concentrations. Plastic is estimated to account for 60-80% of this debris, and is known to be gradually broken down by solar energy and the mechanical action of the sea. This means the majority of the plastic waste are several millimetres in diameter or less, which has made efforts to clean them from the ocean largely unsuccessful. These tiny plastic fragments - microplastics - enter the digestive systems of resident organisms, which are affected either by the physical size of the plastic or its toxicity from adsorbing organic pollutants.<br />
<br />
UCL iGEM proposes a synthetic biology approach for the bioremediation of micro-plastic pollutants within the marine environment, with emphasis on regions of excessive debris accumulation, such as the 'Great pacific garbage patch’ in the North Pacific gyre. <br />
<br />
We intend to engineer ''Escherichia coli'' and marine bacteria ''Roseobacter denitrifican''s & ''Oceanibulbus indolifex'' to degrade plastic, or aggregate it for collection.<br />
<br />
== Detection Module ==<br />
'''Detection of plastics as a trigger for the Aggregation module'''<br />
<br />
Our Detection module will allow our bacteria to detect the presence of plastic. This serves to control the production of our adhesive – curli fibrins – which binds non-specifically to an extraordinary array of surfaces. By producing adhesive only when plastic is present, we prevent our bacteria binding to non-plastic materials.<br />
<br />
== Aggregation Module ==<br />
<br />
'''Curli expression for the aggregation of plastic and production of biofilm'''<br />
<br />
The Aggregation Module confers onto our bacteria the means of plastic adhesion. To implement this we have decided to transform our bacteria with a genetic circuit to produce adhesive proteins called curlis. As curlis are non-specific in the surfaces they bind to, the Detection module will limit their production, unless they are in the presence of plastics.<br />
<br />
== Plastic Degradation ==<br />
'''Multi-copper oxidase/Laccase for the degradation of polyethylene'''<br />
<br />
As an alternative to the Aggregation system, we are also developing an alternative solution – the degradation of plastic. This will investigate enzymes expressed by numerous organisms that have been demonstrated to degrade plastics.<br />
<br />
==Buoyancy Module==<br />
'''Glucose concentration dependent buoyancy for localisation of engineered bacteria within the water column '''<br />
<br />
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, alongside the plastic fragments, and also to enable them to buoy the plastic aggregates.<br />
<br />
==Salt Tolerance==<br />
<br />
'''IrrE a global regulator from ''Deinococcus radiodurans'' for increased salinity tolerance in ''E. coli'''''<br />
<br />
A core module for our project is enabling ''E. coli'' to tolerate the salt content of the ocean; without this ability ''E. coli'' could not survive in a marine environment. Due to the widespread use of ''E. coli'' as a chassis for synthetic biology, this module is being developed to demonstrate that ''E. coli'', as well as marine bacteria, could be used as the chassis for this project.<br />
<br />
==Containment Components==<br />
<br />
'''A novel threefold active biological containment system'''<br />
<br />
UCL iGEM 2012 addresses fundamental barriers to the implementation of traditional biological containment systems. As our project proposes the release of genetically modified bacterium into the environment, we feel it is necessary to contain the risk of spreading genetic information by horizontal gene transfer. To do so we are suggesting a periplasmic nuclease system in order to degrade genetic material in the surrounding area.<br />
<br />
A multi-containment system, consisting of three toxin/anti-toxin pairs - Holin / Anti-Holin Endolysin, Colicin-E3 / Colicin Immunity E3, and Endunuclease EcoRI / Methyltransferase EcoRI will also be considered in our containment system.<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/ResearchTeam:University College London/Research2012-10-26T23:15:05Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headnocover}}<html><img href="http://www.plasticrepublic.org/wikifiles/overview-research.png" /></html><br />
== Project Overview ==<br />
<br />
In many of the world's oceans, currents carry debris and pollution originating from coastlines. This waste accumulates in regional gyres - where ocean currents meet, and can reach extremely high concentrations. Plastic is estimated to account for 60-80% of this debris, and is known to be gradually broken down by solar energy and the mechanical action of the sea. This means the majority of the plastic waste are several millimetres in diameter or less, which has made efforts to clean them from the ocean largely unsuccessful. These tiny plastic fragments - microplastics - enter the digestive systems of resident organisms, which are affected either by the physical size of the plastic or its toxicity from adsorbing organic pollutants.<br />
<br />
UCL iGEM proposes a synthetic biology approach for the bioremediation of micro-plastic pollutants within the marine environment, with emphasis on regions of excessive debris accumulation, such as the 'Great pacific garbage patch’ in the North Pacific gyre. <br />
<br />
We intend to engineer ''Escherichia coli'' and marine bacteria ''Roseobacter denitrifican''s & ''Oceanibulbus indolifex'' to degrade plastic, or aggregate it for collection.<br />
<br />
== Detection Module ==<br />
'''Detection of plastics as a trigger for the Aggregation module'''<br />
<br />
Our Detection module will allow our bacteria to detect the presence of plastic. This serves to control the production of our adhesive – curli fibrins – which binds non-specifically to an extraordinary array of surfaces. By producing adhesive only when plastic is present, we prevent our bacteria binding to non-plastic materials.<br />
<br />
== Aggregation Module ==<br />
<br />
'''Curli expression for the aggregation of plastic and production of biofilm'''<br />
<br />
The Aggregation Module confers onto our bacteria the means of plastic adhesion. To implement this we have decided to transform our bacteria with a genetic circuit to produce adhesive proteins called curlis. As curlis are non-specific in the surfaces they bind to, the Detection module will limit their production, unless they are in the presence of plastics.<br />
<br />
== Plastic Degradation ==<br />
'''Multi-copper oxidase/Laccase for the degradation of polyethylene'''<br />
<br />
As an alternative to the Aggregation system, we are also developing an alternative solution – the degradation of plastic. This will investigate enzymes expressed by numerous organisms that have been demonstrated to degrade plastics.<br />
<br />
==Buoyancy Module==<br />
'''Glucose concentration dependent buoyancy for localisation of engineered bacteria within the water column '''<br />
<br />
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, alongside the plastic fragments, and also to enable them to buoy the plastic aggregates.<br />
<br />
==Salt Tolerance==<br />
<br />
'''IrrE a global regulator from ''Deinococcus radiodurans'' for increased salinity tolerance in ''E. coli'''''<br />
<br />
A core module for our project is enabling ''E. coli'' to tolerate the salt content of the ocean; without this ability ''E. coli'' could not survive in a marine environment. Due to the widespread use of ''E. coli'' as a chassis for synthetic biology, this module is being developed to demonstrate that ''E. coli'', as well as marine bacteria, could be used as the chassis for this project.<br />
<br />
==Containment Components==<br />
<br />
'''A novel threefold active biological containment system'''<br />
<br />
UCL iGEM 2012 addresses fundamental barriers to the implementation of traditional biological containment systems. As our project proposes the release of genetically modified bacterium into the environment, we feel it is necessary to contain the risk of spreading genetic information by horizontal gene transfer. To do so we are suggesting a periplasmic nuclease system in order to degrade genetic material in the surrounding area.<br />
<br />
A multi-containment system, consisting of three toxin/anti-toxin pairs - Holin / Anti-Holin Endolysin, Colicin-E3 / Colicin Immunity E3, and Endunuclease EcoRI / Methyltransferase EcoRI will also be considered in our containment system.<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/ResearchTeam:University College London/Research2012-10-26T23:14:37Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headnocover}}<br />
<html><img href="http://www.plasticrepublic.org/wikifiles/overview-research.png" /></html></html><br />
<br />
== Project Overview ==<br />
<br />
In many of the world's oceans, currents carry debris and pollution originating from coastlines. This waste accumulates in regional gyres - where ocean currents meet, and can reach extremely high concentrations. Plastic is estimated to account for 60-80% of this debris, and is known to be gradually broken down by solar energy and the mechanical action of the sea. This means the majority of the plastic waste are several millimetres in diameter or less, which has made efforts to clean them from the ocean largely unsuccessful. These tiny plastic fragments - microplastics - enter the digestive systems of resident organisms, which are affected either by the physical size of the plastic or its toxicity from adsorbing organic pollutants.<br />
<br />
UCL iGEM proposes a synthetic biology approach for the bioremediation of micro-plastic pollutants within the marine environment, with emphasis on regions of excessive debris accumulation, such as the 'Great pacific garbage patch’ in the North Pacific gyre. <br />
<br />
We intend to engineer ''Escherichia coli'' and marine bacteria ''Roseobacter denitrifican''s & ''Oceanibulbus indolifex'' to degrade plastic, or aggregate it for collection.<br />
<br />
== Detection Module ==<br />
'''Detection of plastics as a trigger for the Aggregation module'''<br />
<br />
Our Detection module will allow our bacteria to detect the presence of plastic. This serves to control the production of our adhesive – curli fibrins – which binds non-specifically to an extraordinary array of surfaces. By producing adhesive only when plastic is present, we prevent our bacteria binding to non-plastic materials.<br />
<br />
== Aggregation Module ==<br />
<br />
'''Curli expression for the aggregation of plastic and production of biofilm'''<br />
<br />
The Aggregation Module confers onto our bacteria the means of plastic adhesion. To implement this we have decided to transform our bacteria with a genetic circuit to produce adhesive proteins called curlis. As curlis are non-specific in the surfaces they bind to, the Detection module will limit their production, unless they are in the presence of plastics.<br />
<br />
== Plastic Degradation ==<br />
'''Multi-copper oxidase/Laccase for the degradation of polyethylene'''<br />
<br />
As an alternative to the Aggregation system, we are also developing an alternative solution – the degradation of plastic. This will investigate enzymes expressed by numerous organisms that have been demonstrated to degrade plastics.<br />
<br />
==Buoyancy Module==<br />
'''Glucose concentration dependent buoyancy for localisation of engineered bacteria within the water column '''<br />
<br />
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, alongside the plastic fragments, and also to enable them to buoy the plastic aggregates.<br />
<br />
==Salt Tolerance==<br />
<br />
'''IrrE a global regulator from ''Deinococcus radiodurans'' for increased salinity tolerance in ''E. coli'''''<br />
<br />
A core module for our project is enabling ''E. coli'' to tolerate the salt content of the ocean; without this ability ''E. coli'' could not survive in a marine environment. Due to the widespread use of ''E. coli'' as a chassis for synthetic biology, this module is being developed to demonstrate that ''E. coli'', as well as marine bacteria, could be used as the chassis for this project.<br />
<br />
==Containment Components==<br />
<br />
'''A novel threefold active biological containment system'''<br />
<br />
UCL iGEM 2012 addresses fundamental barriers to the implementation of traditional biological containment systems. As our project proposes the release of genetically modified bacterium into the environment, we feel it is necessary to contain the risk of spreading genetic information by horizontal gene transfer. To do so we are suggesting a periplasmic nuclease system in order to degrade genetic material in the surrounding area.<br />
<br />
A multi-containment system, consisting of three toxin/anti-toxin pairs - Holin / Anti-Holin Endolysin, Colicin-E3 / Colicin Immunity E3, and Endunuclease EcoRI / Methyltransferase EcoRI will also be considered in our containment system.<br />
<br />
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<br />
iframe{<br />
border:none;<br />
}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/ResearchTeam:University College London/Research2012-10-26T23:12:14Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headnocover}}<br />
<html><img href="http://www.plasticrepublic.org/wikifiles/overview-research.png</html></html><br />
<br />
== Project Overview ==<br />
<br />
In many of the world's oceans, currents carry debris and pollution originating from coastlines. This waste accumulates in regional gyres - where ocean currents meet, and can reach extremely high concentrations. Plastic is estimated to account for 60-80% of this debris, and is known to be gradually broken down by solar energy and the mechanical action of the sea. This means the majority of the plastic waste are several millimetres in diameter or less, which has made efforts to clean them from the ocean largely unsuccessful. These tiny plastic fragments - microplastics - enter the digestive systems of resident organisms, which are affected either by the physical size of the plastic or its toxicity from adsorbing organic pollutants.<br />
<br />
UCL iGEM proposes a synthetic biology approach for the bioremediation of micro-plastic pollutants within the marine environment, with emphasis on regions of excessive debris accumulation, such as the 'Great pacific garbage patch’ in the North Pacific gyre. <br />
<br />
We intend to engineer ''Escherichia coli'' and marine bacteria ''Roseobacter denitrifican''s & ''Oceanibulbus indolifex'' to degrade plastic, or aggregate it for collection.<br />
<br />
== Detection Module ==<br />
'''Detection of plastics as a trigger for the Aggregation module'''<br />
<br />
Our Detection module will allow our bacteria to detect the presence of plastic. This serves to control the production of our adhesive – curli fibrins – which binds non-specifically to an extraordinary array of surfaces. By producing adhesive only when plastic is present, we prevent our bacteria binding to non-plastic materials.<br />
<br />
== Aggregation Module ==<br />
<br />
'''Curli expression for the aggregation of plastic and production of biofilm'''<br />
<br />
The Aggregation Module confers onto our bacteria the means of plastic adhesion. To implement this we have decided to transform our bacteria with a genetic circuit to produce adhesive proteins called curlis. As curlis are non-specific in the surfaces they bind to, the Detection module will limit their production, unless they are in the presence of plastics.<br />
<br />
== Plastic Degradation ==<br />
'''Multi-copper oxidase/Laccase for the degradation of polyethylene'''<br />
<br />
As an alternative to the Aggregation system, we are also developing an alternative solution – the degradation of plastic. This will investigate enzymes expressed by numerous organisms that have been demonstrated to degrade plastics.<br />
<br />
==Buoyancy Module==<br />
'''Glucose concentration dependent buoyancy for localisation of engineered bacteria within the water column '''<br />
<br />
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, alongside the plastic fragments, and also to enable them to buoy the plastic aggregates.<br />
<br />
==Salt Tolerance==<br />
<br />
'''IrrE a global regulator from ''Deinococcus radiodurans'' for increased salinity tolerance in ''E. coli'''''<br />
<br />
A core module for our project is enabling ''E. coli'' to tolerate the salt content of the ocean; without this ability ''E. coli'' could not survive in a marine environment. Due to the widespread use of ''E. coli'' as a chassis for synthetic biology, this module is being developed to demonstrate that ''E. coli'', as well as marine bacteria, could be used as the chassis for this project.<br />
<br />
==Containment Components==<br />
<br />
'''A novel threefold active biological containment system'''<br />
<br />
UCL iGEM 2012 addresses fundamental barriers to the implementation of traditional biological containment systems. As our project proposes the release of genetically modified bacterium into the environment, we feel it is necessary to contain the risk of spreading genetic information by horizontal gene transfer. To do so we are suggesting a periplasmic nuclease system in order to degrade genetic material in the surrounding area.<br />
<br />
A multi-containment system, consisting of three toxin/anti-toxin pairs - Holin / Anti-Holin Endolysin, Colicin-E3 / Colicin Immunity E3, and Endunuclease EcoRI / Methyltransferase EcoRI will also be considered in our containment system.<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/LabBook/Week17Team:University College London/LabBook/Week172012-10-26T23:07:31Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headimg|coverpicture=images/a/a1/Ucl2012-labbook-title.png}}<html><script type="text/javascript" src="https://2012.igem.org/wiki/index.php?title=Team:University_College_London/js/labbookjs&amp;action=raw&amp;ctype=text/js"></script><br />
</html>{{:Team:University_College_London/templates/labbookmenu}}<html><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Ucl2012-labbook-monfri.png" /><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-2.png"" /><div class="experimentContent"><br />
</html><br />
==Material from cells containing our Biosafety BioBrick is not able to transform commercial competent cells==<br />
<br />
We used sonication to disrupt three cell types: unmodified W3110 strain E. coli and W3110 strains harbouring chloramphenical resistance plasmids encoding a laccase (BBa_K729006) or the DsbA-Nuclease (BBa_K729019). All three strains were grown to OD600= 2.0 prior to sonication, in 2mL LB broth which contained 100ug/mL chloramphenical for plasmid-harbouring cells.<br />
<br />
After sonication, disruptates were incubated for 10min at room temperature. 5uL of the disruptate was used to transform an aliquot of TOP10 chemically competent cells, following manufacturer instructions. As a control, we also spread 20uL of the disruptate material directly onto LB agar plates with and without 100ug/mL Chloramphenical. No colonies were observed on the control plates for any of the three strains (data not shown), indicating sonication had successfully disrupted all cells.<br />
<br />
[[File:20121025-Bouran.S.NucleaseExpPlates.25.10.12-2.jpg|thumb|center|600px|alt=|'''Figure 1.''' Transformation of comercially competent TOP10 E. Coli cells with '''W3110:''' unmodified E. coli W3110 disruptate, '''Laccase''': W3110 harbouring the BBa_K729006 laccase plasmid and '''Nuclease''': E. coli W3110 harbouring the BBa_K729019 nuclease plasmid. ]]<br />
<br />
As expected, transformation of TOP10 cells with disruptate of unmodified W3110 did not yield any cells able to grow on chloramphenicol plates (FigXA). In contrast transformation with disruptate of W3110 harbouring the BBa_K729006 laccase plasmid yielded many TOP10 colonies on chloramphenicol plates (FigXA), indicating that plasmid DNA in the disruptate was able to transform TOP10 cells.<br />
<br />
Transformation with disruptate of W3110 harbouring the BBa_K729019 nuclease plasmid yielded no colonies on chloramphenicol plates (FigXC). BBa_K729004 differs from BBa_K729006 only in that it contains an ORF encoding the periplasmic nuclease instead of laccase. As such, we conclude the periplasmic nuclease has sufficient DNAase activity in the disruptate to reduce the amount of plasmid DNA present to below the threshold capable of transforming TOP10 cells.<br />
<br />
We suggest periplasmic nuclease expression provides a promising strategy for preventing transfer of genetically modified DNA. This is particularly valuable for the application of synthetic biology in environmental contexts.<br />
<html><br />
</div><div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-3.png" /><br />
<div class="experimentContent"></html><br />
== 17-3==<br />
<html></div><br />
<div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-4.png"" /><div class="experimentContent"><br />
</html><br />
== 17-4==<br />
<br />
<br />
{| class="bigtable"<br />
|-<br />
! Time!! Run 1 !! Run 2 !! Run 3<br />
|-<br />
| 0 ||0.001 || 0.001|| 0.001<br />
|-<br />
| 1 || 0.002 || 0.003||0.004<br />
|-<br />
| 2 || 0.008|| 0.007 || 0.008<br />
|-<br />
| 3 || 0.02 || 0.019|| 0.019<br />
|-<br />
| 4 || 0.029 || 0.026 ||0.027<br />
|-<br />
| 5 || 0.06|| 0.059 || 0.062<br />
|-<br />
| 6 || 0.087|| 0.089|| 0.089<br />
|-<br />
| 7 || 0.184|| 0.185 || 0.183<br />
|-<br />
| 8 || 0.302|| 0.297 || 0.292<br />
|-<br />
| 9 || 0.669|| 0.678 || 0.675<br />
|}<br />
<html><br />
</div><div class="experiment"></div></html><br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/Module_2/Shear_DeviceTeam:University College London/Module 2/Shear Device2012-10-26T23:05:38Z<p>PhilippBoeing: /* Status */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=training}}<br />
<br />
= Module 2: Aggregation =<br />
{{:Team:University_College_London/templates/module2menu}}<br />
== The Demand ==<br />
It is imperative that BioBricks submitted to the parts registry are well characterised, but we noticed that experiments previously done for binding cells to surfaces are very qualitative, they will highlight the presence of cells but there is no ability to draw comparisons or conclusions beyond that. As a means of really characterising our Aggregation module well, we wanted to be able to quantify the strength of the binding that the cells containing our construct would be able to infer on the target plastics.<br />
<br />
<html><div style="margin:15px;float:right"><iframe width="300" height="169" src="http://www.youtube.com/embed/tgCThLXOh7k" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
== The Experts ==<br />
We would need to expert some force on the cells and be able to measure this and the response of the organisms. Initial research suggested that the use of shear force would allow for our requirements, and so we consulted an expert in miniaturisation and shear from the Department of Biochemical Engineering as well as the Department of Biochemical and Chemical Engineering’s Rapid Design and Fabrication Facility (RDFF). These fruitful sessions lead to the production of three preliminary design concepts (which can be seen as Fig.1a-c below). <br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/1/13/Ucl2012-Shear1.png" /></html><br />
<br />
All 3 designs work on the principal of using a rotating head plate above a disc of plastic which has had cells adhered to it, to exert variable shear force across the surface of the disc. But all three have design and configurational differences which affect the mode of operation and how the magnitude of force is altered (further details are given for the chosen design below).<br />
<br />
== Refinement ==<br />
Further research was conducted into the three crude designs with a view to whittling it down to a single candidate. This investigation focused on two main areas, firstly what work had been done previously, and therefore which of the devices would have available information from the literature. Additionally, and arguably more importantly what would be feasible based on the time constraints of the competition, the capabilities of the RDFF and the laboratories that were at our disposal.<br />
<br />
== The Design ==<br />
The chosen design is based on Fig1a. (above). Further work with the RDFF yielded the following technical drawings (Fig2.)<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/88/Ucl2012-shear2.png" /></html><br />
<br />
== The Science ==<br />
As mentioned above, this device has been designed on the principal of quantifying the adhesion between the cells containing our aggregation construct and the plastics which it targets.<br />
In the centre of the plastic disc, at a fixed rotational speed, there is a region of lower shear where cells remain attached to the surface. As you increase the distance from the centre of the disc the shear stress increases linearly (according to Eqn1. below), and so eventually the cells can no longer remain attached. The interface between the two can be used to calculate a critical shear stress, the point at which the cells adhesive strength is outweighed by the shear being exerted.<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/8f/Ucl2012-shear3.png" /></html><br />
<br />
By back calculating, we can actually draw a cut off line, showing where we need cells to be adhering to in order to withstand the forces exerted in the marine environment<br />
<br />
== Status ==<br />
Currently the device pictured is under construction by the RDFF. We have carried out some work with a rudimentary version of the device (see video) as a means of proving the concept and highlighting the potential power of this new tool.<br />
<br />
<html><iframe width="560" height="315" src="http://www.youtube.com/embed/LWQSzA42t5w" frameborder="0" allowfullscreen></iframe></html></div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/Module_2/Shear_DeviceTeam:University College London/Module 2/Shear Device2012-10-26T23:05:30Z<p>PhilippBoeing: /* Status */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=training}}<br />
<br />
= Module 2: Aggregation =<br />
{{:Team:University_College_London/templates/module2menu}}<br />
== The Demand ==<br />
It is imperative that BioBricks submitted to the parts registry are well characterised, but we noticed that experiments previously done for binding cells to surfaces are very qualitative, they will highlight the presence of cells but there is no ability to draw comparisons or conclusions beyond that. As a means of really characterising our Aggregation module well, we wanted to be able to quantify the strength of the binding that the cells containing our construct would be able to infer on the target plastics.<br />
<br />
<html><div style="margin:15px;float:right"><iframe width="300" height="169" src="http://www.youtube.com/embed/tgCThLXOh7k" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
== The Experts ==<br />
We would need to expert some force on the cells and be able to measure this and the response of the organisms. Initial research suggested that the use of shear force would allow for our requirements, and so we consulted an expert in miniaturisation and shear from the Department of Biochemical Engineering as well as the Department of Biochemical and Chemical Engineering’s Rapid Design and Fabrication Facility (RDFF). These fruitful sessions lead to the production of three preliminary design concepts (which can be seen as Fig.1a-c below). <br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/1/13/Ucl2012-Shear1.png" /></html><br />
<br />
All 3 designs work on the principal of using a rotating head plate above a disc of plastic which has had cells adhered to it, to exert variable shear force across the surface of the disc. But all three have design and configurational differences which affect the mode of operation and how the magnitude of force is altered (further details are given for the chosen design below).<br />
<br />
== Refinement ==<br />
Further research was conducted into the three crude designs with a view to whittling it down to a single candidate. This investigation focused on two main areas, firstly what work had been done previously, and therefore which of the devices would have available information from the literature. Additionally, and arguably more importantly what would be feasible based on the time constraints of the competition, the capabilities of the RDFF and the laboratories that were at our disposal.<br />
<br />
== The Design ==<br />
The chosen design is based on Fig1a. (above). Further work with the RDFF yielded the following technical drawings (Fig2.)<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/88/Ucl2012-shear2.png" /></html><br />
<br />
== The Science ==<br />
As mentioned above, this device has been designed on the principal of quantifying the adhesion between the cells containing our aggregation construct and the plastics which it targets.<br />
In the centre of the plastic disc, at a fixed rotational speed, there is a region of lower shear where cells remain attached to the surface. As you increase the distance from the centre of the disc the shear stress increases linearly (according to Eqn1. below), and so eventually the cells can no longer remain attached. The interface between the two can be used to calculate a critical shear stress, the point at which the cells adhesive strength is outweighed by the shear being exerted.<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/8f/Ucl2012-shear3.png" /></html><br />
<br />
By back calculating, we can actually draw a cut off line, showing where we need cells to be adhering to in order to withstand the forces exerted in the marine environment<br />
<br />
== Status ==<br />
Currently the device pictured is under construction by the RDFF. We have carried out some work with a rudimentary version of the device (see video) as a means of proving the concept and highlighting the potential power of this new tool.<br />
<html><iframe width="560" height="315" src="http://www.youtube.com/embed/LWQSzA42t5w" frameborder="0" allowfullscreen></iframe></html></div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/Module_2/Shear_DeviceTeam:University College London/Module 2/Shear Device2012-10-26T23:03:54Z<p>PhilippBoeing: /* The Demand */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=training}}<br />
<br />
= Module 2: Aggregation =<br />
{{:Team:University_College_London/templates/module2menu}}<br />
== The Demand ==<br />
It is imperative that BioBricks submitted to the parts registry are well characterised, but we noticed that experiments previously done for binding cells to surfaces are very qualitative, they will highlight the presence of cells but there is no ability to draw comparisons or conclusions beyond that. As a means of really characterising our Aggregation module well, we wanted to be able to quantify the strength of the binding that the cells containing our construct would be able to infer on the target plastics.<br />
<br />
<html><div style="margin:15px;float:right"><iframe width="300" height="169" src="http://www.youtube.com/embed/tgCThLXOh7k" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
== The Experts ==<br />
We would need to expert some force on the cells and be able to measure this and the response of the organisms. Initial research suggested that the use of shear force would allow for our requirements, and so we consulted an expert in miniaturisation and shear from the Department of Biochemical Engineering as well as the Department of Biochemical and Chemical Engineering’s Rapid Design and Fabrication Facility (RDFF). These fruitful sessions lead to the production of three preliminary design concepts (which can be seen as Fig.1a-c below). <br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/1/13/Ucl2012-Shear1.png" /></html><br />
<br />
All 3 designs work on the principal of using a rotating head plate above a disc of plastic which has had cells adhered to it, to exert variable shear force across the surface of the disc. But all three have design and configurational differences which affect the mode of operation and how the magnitude of force is altered (further details are given for the chosen design below).<br />
<br />
== Refinement ==<br />
Further research was conducted into the three crude designs with a view to whittling it down to a single candidate. This investigation focused on two main areas, firstly what work had been done previously, and therefore which of the devices would have available information from the literature. Additionally, and arguably more importantly what would be feasible based on the time constraints of the competition, the capabilities of the RDFF and the laboratories that were at our disposal.<br />
<br />
== The Design ==<br />
The chosen design is based on Fig1a. (above). Further work with the RDFF yielded the following technical drawings (Fig2.)<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/88/Ucl2012-shear2.png" /></html><br />
<br />
== The Science ==<br />
As mentioned above, this device has been designed on the principal of quantifying the adhesion between the cells containing our aggregation construct and the plastics which it targets.<br />
In the centre of the plastic disc, at a fixed rotational speed, there is a region of lower shear where cells remain attached to the surface. As you increase the distance from the centre of the disc the shear stress increases linearly (according to Eqn1. below), and so eventually the cells can no longer remain attached. The interface between the two can be used to calculate a critical shear stress, the point at which the cells adhesive strength is outweighed by the shear being exerted.<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/8f/Ucl2012-shear3.png" /></html><br />
<br />
By back calculating, we can actually draw a cut off line, showing where we need cells to be adhering to in order to withstand the forces exerted in the marine environment<br />
<br />
== Status ==<br />
Currently the device pictured is under construction by the RDFF. We have carried out some work with a rudimentary version of the device (see video) as a means of proving the concept and highlighting the potential power of this new tool.</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/Module_2/Shear_DeviceTeam:University College London/Module 2/Shear Device2012-10-26T23:03:19Z<p>PhilippBoeing: /* The Experts */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=training}}<br />
<br />
= Module 2: Aggregation =<br />
{{:Team:University_College_London/templates/module2menu}}<br />
== The Demand ==<br />
It is imperative that BioBricks submitted to the parts registry are well characterised, but we noticed that experiments previously done for binding cells to surfaces are very qualitative, they will highlight the presence of cells but there is no ability to draw comparisons or conclusions beyond that. As a means of really characterising our Aggregation module well, we wanted to be able to quantify the strength of the binding that the cells containing our construct would be able to infer on the target plastics.<br />
<br />
<html><div style="margin:15px;float:right"><iframe width="300" height="169" src="http://www.youtube.com/embed/tgCThLXOh7k" frameborder="0" allowfullscreen></div></iframe></html><br />
== The Experts ==<br />
We would need to expert some force on the cells and be able to measure this and the response of the organisms. Initial research suggested that the use of shear force would allow for our requirements, and so we consulted an expert in miniaturisation and shear from the Department of Biochemical Engineering as well as the Department of Biochemical and Chemical Engineering’s Rapid Design and Fabrication Facility (RDFF). These fruitful sessions lead to the production of three preliminary design concepts (which can be seen as Fig.1a-c below). <br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/1/13/Ucl2012-Shear1.png" /></html><br />
<br />
All 3 designs work on the principal of using a rotating head plate above a disc of plastic which has had cells adhered to it, to exert variable shear force across the surface of the disc. But all three have design and configurational differences which affect the mode of operation and how the magnitude of force is altered (further details are given for the chosen design below).<br />
<br />
== Refinement ==<br />
Further research was conducted into the three crude designs with a view to whittling it down to a single candidate. This investigation focused on two main areas, firstly what work had been done previously, and therefore which of the devices would have available information from the literature. Additionally, and arguably more importantly what would be feasible based on the time constraints of the competition, the capabilities of the RDFF and the laboratories that were at our disposal.<br />
<br />
== The Design ==<br />
The chosen design is based on Fig1a. (above). Further work with the RDFF yielded the following technical drawings (Fig2.)<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/88/Ucl2012-shear2.png" /></html><br />
<br />
== The Science ==<br />
As mentioned above, this device has been designed on the principal of quantifying the adhesion between the cells containing our aggregation construct and the plastics which it targets.<br />
In the centre of the plastic disc, at a fixed rotational speed, there is a region of lower shear where cells remain attached to the surface. As you increase the distance from the centre of the disc the shear stress increases linearly (according to Eqn1. below), and so eventually the cells can no longer remain attached. The interface between the two can be used to calculate a critical shear stress, the point at which the cells adhesive strength is outweighed by the shear being exerted.<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/8f/Ucl2012-shear3.png" /></html><br />
<br />
By back calculating, we can actually draw a cut off line, showing where we need cells to be adhering to in order to withstand the forces exerted in the marine environment<br />
<br />
== Status ==<br />
Currently the device pictured is under construction by the RDFF. We have carried out some work with a rudimentary version of the device (see video) as a means of proving the concept and highlighting the potential power of this new tool.</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/Module_2/Shear_DeviceTeam:University College London/Module 2/Shear Device2012-10-26T22:56:56Z<p>PhilippBoeing: /* The Science */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=training}}<br />
<br />
= Module 2: Aggregation =<br />
{{:Team:University_College_London/templates/module2menu}}<br />
== The Demand ==<br />
It is imperative that BioBricks submitted to the parts registry are well characterised, but we noticed that experiments previously done for binding cells to surfaces are very qualitative, they will highlight the presence of cells but there is no ability to draw comparisons or conclusions beyond that. As a means of really characterising our Aggregation module well, we wanted to be able to quantify the strength of the binding that the cells containing our construct would be able to infer on the target plastics.<br />
<br />
== The Experts ==<br />
We would need to expert some force on the cells and be able to measure this and the response of the organisms. Initial research suggested that the use of shear force would allow for our requirements, and so we consulted an expert in miniaturisation and shear from the Department of Biochemical Engineering as well as the Department of Biochemical and Chemical Engineering’s Rapid Design and Fabrication Facility (RDFF). These fruitful sessions lead to the production of three preliminary design concepts (which can be seen as Fig.1a-c below). <br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/1/13/Ucl2012-Shear1.png" /></html><br />
<br />
All 3 designs work on the principal of using a rotating head plate above a disc of plastic which has had cells adhered to it, to exert variable shear force across the surface of the disc. But all three have design and configurational differences which affect the mode of operation and how the magnitude of force is altered (further details are given for the chosen design below).<br />
<br />
== Refinement ==<br />
Further research was conducted into the three crude designs with a view to whittling it down to a single candidate. This investigation focused on two main areas, firstly what work had been done previously, and therefore which of the devices would have available information from the literature. Additionally, and arguably more importantly what would be feasible based on the time constraints of the competition, the capabilities of the RDFF and the laboratories that were at our disposal.<br />
<br />
== The Design ==<br />
The chosen design is based on Fig1a. (above). Further work with the RDFF yielded the following technical drawings (Fig2.)<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/88/Ucl2012-shear2.png" /></html><br />
<br />
== The Science ==<br />
As mentioned above, this device has been designed on the principal of quantifying the adhesion between the cells containing our aggregation construct and the plastics which it targets.<br />
In the centre of the plastic disc, at a fixed rotational speed, there is a region of lower shear where cells remain attached to the surface. As you increase the distance from the centre of the disc the shear stress increases linearly (according to Eqn1. below), and so eventually the cells can no longer remain attached. The interface between the two can be used to calculate a critical shear stress, the point at which the cells adhesive strength is outweighed by the shear being exerted.<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/8f/Ucl2012-shear3.png" /></html><br />
<br />
By back calculating, we can actually draw a cut off line, showing where we need cells to be adhering to in order to withstand the forces exerted in the marine environment<br />
<br />
== Status ==<br />
Currently the device pictured is under construction by the RDFF. We have carried out some work with a rudimentary version of the device (see video) as a means of proving the concept and highlighting the potential power of this new tool.</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/Module_2/Shear_DeviceTeam:University College London/Module 2/Shear Device2012-10-26T22:56:34Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=training}}<br />
<br />
= Module 2: Aggregation =<br />
{{:Team:University_College_London/templates/module2menu}}<br />
== The Demand ==<br />
It is imperative that BioBricks submitted to the parts registry are well characterised, but we noticed that experiments previously done for binding cells to surfaces are very qualitative, they will highlight the presence of cells but there is no ability to draw comparisons or conclusions beyond that. As a means of really characterising our Aggregation module well, we wanted to be able to quantify the strength of the binding that the cells containing our construct would be able to infer on the target plastics.<br />
<br />
== The Experts ==<br />
We would need to expert some force on the cells and be able to measure this and the response of the organisms. Initial research suggested that the use of shear force would allow for our requirements, and so we consulted an expert in miniaturisation and shear from the Department of Biochemical Engineering as well as the Department of Biochemical and Chemical Engineering’s Rapid Design and Fabrication Facility (RDFF). These fruitful sessions lead to the production of three preliminary design concepts (which can be seen as Fig.1a-c below). <br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/1/13/Ucl2012-Shear1.png" /></html><br />
<br />
All 3 designs work on the principal of using a rotating head plate above a disc of plastic which has had cells adhered to it, to exert variable shear force across the surface of the disc. But all three have design and configurational differences which affect the mode of operation and how the magnitude of force is altered (further details are given for the chosen design below).<br />
<br />
== Refinement ==<br />
Further research was conducted into the three crude designs with a view to whittling it down to a single candidate. This investigation focused on two main areas, firstly what work had been done previously, and therefore which of the devices would have available information from the literature. Additionally, and arguably more importantly what would be feasible based on the time constraints of the competition, the capabilities of the RDFF and the laboratories that were at our disposal.<br />
<br />
== The Design ==<br />
The chosen design is based on Fig1a. (above). Further work with the RDFF yielded the following technical drawings (Fig2.)<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/88/Ucl2012-shear2.png" /></html><br />
<br />
== The Science ==<br />
As mentioned above, this device has been designed on the principal of quantifying the adhesion between the cells containing our aggregation construct and the plastics which it targets.<br />
In the centre of the plastic disc, at a fixed rotational speed, there is a region of lower shear where cells remain attached to the surface. As you increase the distance from the centre of the disc the shear stress increases linearly (according to Eqn1. below), and so eventually the cells can no longer remain attached. The interface between the two can be used to calculate a critical shear stress, the point at which the cells adhesive strength is outweighed by the shear being exerted.<br />
<br />
<html><img src="https://static.igem.org/mediawiki/2012/8/8f/Ucl2012-shear3.png" /></html><br />
<br />
�By back calculating, we can actually draw a cut off line, showing where we need cells to be adhering to in order to withstand the forces exerted in the marine environment <br />
<br />
== Status ==<br />
Currently the device pictured is under construction by the RDFF. We have carried out some work with a rudimentary version of the device (see video) as a means of proving the concept and highlighting the potential power of this new tool.</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/Module_2/Shear_DeviceTeam:University College London/Module 2/Shear Device2012-10-26T22:54:55Z<p>PhilippBoeing: /* The Story of the Shear Device */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=training}}<br />
<br />
= Module 2: Aggregation =<br />
{{:Team:University_College_London/templates/module2menu}}</div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-shear3.pngFile:Ucl2012-shear3.png2012-10-26T22:54:07Z<p>PhilippBoeing: </p>
<hr />
<div></div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-shear2.pngFile:Ucl2012-shear2.png2012-10-26T22:50:41Z<p>PhilippBoeing: </p>
<hr />
<div></div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-Shear1.pngFile:Ucl2012-Shear1.png2012-10-26T22:49:36Z<p>PhilippBoeing: uploaded a new version of &quot;File:Ucl2012-Shear1.png&quot;</p>
<hr />
<div></div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-Shear1.pngFile:Ucl2012-Shear1.png2012-10-26T22:45:50Z<p>PhilippBoeing: </p>
<hr />
<div></div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/SponsorsTeam:University College London/Sponsors2012-10-26T22:41:48Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=portico}}<br />
<br />
= Sponsors =<br />
<br />
We are very grateful for the strong support and generous contributions of our sponsors, without whom this project would not be possible. <br /><br />
<br />
<html><div align="center"><br />
<a href="http://www.engineering.ucl.ac.uk/" title="FacultyEng" target="_blank"><img src="https://static.igem.org/mediawiki/2012/f/f6/Ucl2012_sponsor_facultyeng.png" /></a><br />
<a href="http://www.autodesk.com" title="Autodesk" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/a/a9/Ucl2012-sponsor-autodesk.png" /></a> <a href="http://www.promega.com" title="Promega" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/9/96/Ucl2012-sponsor-promega.png" /></a><br /><br />
<a href="http://www.mathworks.com" title="Mathworks" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/8/86/Ucl2012-sponsor-mathworks.png" /></a> <a href="http://www.geneious.com" title="Geneious" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/2/2e/Ucl2012-sponsor-geneious.png" /></a><br /><br />
<a href="http://www.biosilta.com" title="Biosilta" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/4/40/Ucl2012-sponsor-biosilta.png" /></a><a href="http://www.synthace.com" title="Synthace" target="_blank"><img src="https://static.igem.org/mediawiki/2012/3/3e/Ucl2012-sponsor-synthace.png" /></a><a href="http://www.clcbio.com" title="CLC" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/5/53/Ucl2012-sponsor-clc.png" /></a><br /><a href="http://www.wellcome.ac.uk" title="Wellcome Trust" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/0/00/Ucl2012-sponsor-wellcome.png" /></a><a href="http://www.lonza.com" title="Lonza" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/8/8d/Ucl2012-sponsor-lonza.png" /></a><a <br />
href="http://www.mendeley.com/" title="Mendeley" target="_blank"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ucl2012-sponsor-mendeley.png" /></a><br />
<br /><a href="http://www.ucl.ac.uk/biochemeng/" title="DeptBioEng" target="_blank"><img src="https://static.igem.org/mediawiki/2012/c/cc/Ucl2012_sponsor_depbiochemeng.png" /></a><a href="http://www.erasynbio.net"><img src="https://static.igem.org/mediawiki/2012/2/2b/Ucl2012-sponsors-erasynbio.png" /></a></div></html><br />
<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-sponsors-erasynbio.pngFile:Ucl2012-sponsors-erasynbio.png2012-10-26T22:41:03Z<p>PhilippBoeing: </p>
<hr />
<div></div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012_sponsor_depbiochemeng.pngFile:Ucl2012 sponsor depbiochemeng.png2012-10-26T22:40:06Z<p>PhilippBoeing: uploaded a new version of &quot;File:Ucl2012 sponsor depbiochemeng.png&quot;</p>
<hr />
<div></div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/LabBook/Week17Team:University College London/LabBook/Week172012-10-26T22:28:48Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headimg|coverpicture=images/a/a1/Ucl2012-labbook-title.png}}<html><script type="text/javascript" src="https://2012.igem.org/wiki/index.php?title=Team:University_College_London/js/labbookjs&amp;action=raw&amp;ctype=text/js"></script><br />
</html>{{:Team:University_College_London/templates/labbookmenu}}<html><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Ucl2012-labbook-monfri.png" /><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-2.png"" /><div class="experimentContent"><br />
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==Material from cells containing our Biosafety BioBrick is not able to transform commercial competent cells==<br />
<br />
We used sonication to disrupt three cell types: unmodified W3110 strain E. coli and W3110 strains harbouring chloramphenical resistance plasmids encoding a laccase (BBa_K729006) or the DsbA-Nuclease (BBa_K729019). All three strains were grown to OD600= 2.0 prior to sonication, in 2mL LB broth which contained 100ug/mL chloramphenical for plasmid-harbouring cells.<br />
<br />
After sonication, disruptates were incubated for 10min at room temperature. 5uL of the disruptate was used to transform an aliquot of TOP10 chemically competent cells, following manufacturer instructions. As a control, we also spread 20uL of the disruptate material directly onto LB agar plates with and without 100ug/mL Chloramphenical. No colonies were observed on the control plates for any of the three strains (data not shown), indicating sonication had successfully disrupted all cells.<br />
<br />
[[File:20121025-Bouran.S.NucleaseExpPlates.25.10.12-2.jpg|thumb|center|600px|alt=|'''Figure 1.''' Transformation of comercially competent TOP10 E. Coli cells with '''W3110:''' unmodified E. coli W3110 disruptate, '''Laccase''': W3110 harbouring the BBa_K729006 laccase plasmid and '''Nuclease''': E. coli W3110 harbouring the BBa_K729019 nuclease plasmid. ]]<br />
<br />
As expected, transformation of TOP10 cells with disruptate of unmodified W3110 did not yield any cells able to grow on chloramphenicol plates (FigXA). In contrast transformation with disruptate of W3110 harbouring the BBa_K729006 laccase plasmid yielded many TOP10 colonies on chloramphenicol plates (FigXA), indicating that plasmid DNA in the disruptate was able to transform TOP10 cells.<br />
<br />
Transformation with disruptate of W3110 harbouring the BBa_K729019 nuclease plasmid yielded no colonies on chloramphenicol plates (FigXC). BBa_K729004 differs from BBa_K729006 only in that it contains an ORF encoding the periplasmic nuclease instead of laccase. As such, we conclude the periplasmic nuclease has sufficient DNAase activity in the disruptate to reduce the amount of plasmid DNA present to below the threshold capable of transforming TOP10 cells.<br />
<br />
We suggest periplasmic nuclease expression provides a promising strategy for preventing transfer of genetically modified DNA. This is particularly valuable for the application of synthetic biology in environmental contexts.<br />
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<img src="http://www.plasticrepublic.org/wikifiles/lab17-3.png" /><br />
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== 17-3==<br />
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<div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-4.png"" /><div class="experimentContent"><br />
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== 17-4==<br />
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<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/LabBook/Week17Team:University College London/LabBook/Week172012-10-26T16:57:42Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/headimg|coverpicture=images/a/a1/Ucl2012-labbook-title.png}}<html><script type="text/javascript" src="https://2012.igem.org/wiki/index.php?title=Team:University_College_London/js/labbookjs&amp;action=raw&amp;ctype=text/js"></script><br />
</html>{{:Team:University_College_London/templates/labbookmenu}}<html><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Ucl2012-labbook-monfri.png" /><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-1.png" /><br />
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== 17-1==<br />
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== 17-2==<br />
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== 17-3==<br />
<html></div><br />
<div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-4.png"" /><div class="experimentContent"><br />
</html><br />
== 17-4==<br />
<html><br />
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{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/LabBook/Week17Team:University College London/LabBook/Week172012-10-26T16:49:39Z<p>PhilippBoeing: </p>
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<div>{{:Team:University_College_London/templates/headimg|coverpicture=images/a/a1/Ucl2012-labbook-title.png}}<html><script type="text/javascript" src="https://2012.igem.org/wiki/index.php?title=Team:University_College_London/js/labbookjs&amp;action=raw&amp;ctype=text/js"></script><br />
</html>{{:Team:University_College_London/templates/labbookmenu}}<html><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Ucl2012-labbook-monfri.png" /><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-1.png" /><br />
<div class="experimentContent"></html><br />
= 17-1=<br />
<html></div><br />
<div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-2.png"" /><div class="experimentContent"><br />
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= 17-2=<br />
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</div><div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-3.png" /><br />
<div class="experimentContent"></html><br />
= 17-3=<br />
<html></div><br />
<div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-4.png"" /><div class="experimentContent"><br />
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= 17-4=<br />
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{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/LabBook/Week17Team:University College London/LabBook/Week172012-10-26T16:49:16Z<p>PhilippBoeing: Created page with "{{:Team:University_College_London/templates/headimg|coverpicture=images/a/a1/Ucl2012-labbook-title.png}}<html><script type="text/javascript" src="https://2012.igem.org/wiki/index...."</p>
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<div>{{:Team:University_College_London/templates/headimg|coverpicture=images/a/a1/Ucl2012-labbook-title.png}}<html><script type="text/javascript" src="https://2012.igem.org/wiki/index.php?title=Team:University_College_London/js/labbookjs&amp;action=raw&amp;ctype=text/js"></script><br />
</html>{{:Team:University_College_London/templates/labbookmenu}}<html><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Ucl2012-labbook-monfri.png" /><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-1.png" /><br />
<div class="experimentContent"></html><br />
= 17-1=<br />
<html></div><br />
<div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-2.png"" /><div class="experimentContent"><br />
</html><br />
= 17-2=<br />
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</div><div class="experiment"></div></html><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-3.png" /><br />
<div class="experimentContent"></html><br />
= 17-3=<br />
<html></div><br />
<div class="experiment"></div><br />
<img src="http://www.plasticrepublic.org/wikifiles/lab17-4.png"" /><div class="experimentContent"><br />
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= 17-4=<br />
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{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/templates/labbookmenuTeam:University College London/templates/labbookmenu2012-10-26T16:46:39Z<p>PhilippBoeing: </p>
<hr />
<div><div id="labbookmenu">'''[[Team:University_College_London/LabBook/Week3|Week 3]] | [[Team:University_College_London/LabBook/Week4|Week 4]] | [[Team:University_College_London/LabBook/Week5|Week 5]] | [[Team:University_College_London/LabBook/Week6|Week 6]] | [[Team:University_College_London/LabBook/Week7|Week 7]] | [[Team:University_College_London/LabBook/Week8|Week 8]] | [[Team:University_College_London/LabBook/Week9|Week 9]] | [[Team:University_College_London/LabBook/Week10|Week 10]] | [[Team:University_College_London/LabBook/Week11|Week 11]] | [[Team:University_College_London/LabBook/Week12|Week 12]] | [[Team:University_College_London/LabBook/Week13|Week 13]] | [[Team:University_College_London/LabBook/Week14|Week 14]] | [[Team:University_College_London/LabBook/Week15|Week 15]] | [[Team:University_College_London/LabBook/Week16|Week 16]] | [[Team:University_College_London/LabBook/Week17|Week 17]]'''</div></div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/cssTeam:University College London/css2012-10-26T16:15:23Z<p>PhilippBoeing: </p>
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<br />
iframe{<br />
border:none;<br />
}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/HumanPractice/MOYMTeam:University College London/HumanPractice/MOYM2012-10-26T16:15:03Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=moym}}<br />
<br />
<html><div style="float:right"><iframe width="560" height="315" src="http://www.youtube.com/embed/GhjOQCk8E_k?list=UUQPIppvUH1aw9iqjdFEdp3g&amp;hl=en_US" frameborder="0" allowfullscreen></iframe></div></html><br />
= Meeting Of Young Minds Debate =<br />
<br />
== Meeting Of Young Minds ==<br />
At the beginning of June, the [http://www.rathenau.nl/en.html Rathenau Instituut] (a Dutch body which promotes the formation of political and public opinion on science and technology) put out a call for proposals for a debate, the Meeting of Young Minds, to be held on the Friday evening preceding the African-European jamboree. <br />
<br />
The first Meeting of Young Minds debate, which [http://www.youtube.com/watch?v=g5YPUFUayTo took place in 2011], pitted iGEM teams against representatives from Dutch youth political parties in a meeting of ‘future scientists and future politicians’ to discuss the ‘promises and perils of synthetic biology’. For Meeting of Young Minds 2012, iGEM teams were invivted to submit their own themes for debate. Our proposal ‘A synthetic biology future, a synthetic biology crisis’ was one of two themes selected.<br />
<br />
== Our proposal ==<br />
We decided that we wanted our proposal to have some link to our project, hoping that we might gain some new perspectives from the debate. At this point in the summer, we knew <i>what</i> our project was going to be, but not <i>how</i> it was going to work, so we came up with the idea of a debate broadly based around geoengineering – deliberate large-scale scientific projects to address or remedy the environmental impact of human activities. <br />
<br />
Early ideas included discussion on the ethics of syn-bio geoengineering and the rights and responsibilities relating to such large scale projects. However, our research quickly revealed that these topics had been already been well-publicized and thoroughly discussed. We decided to try and avoid the tired and abstract moral/ethical questions, and bring our debate into a more concrete setting by using a ‘crisis scenario’. <br />
<br />
== Crisis scenario ==<br />
The crisis scenario was inspired by the Rathenau Institute’s [http://www.rathenau.nl/themas/thema/project/synthetische-biologie/synbio-futures.html Synbio futures], a series of ‘techno-moral vignettes’ – short stories imagining possible futures in which synthetic biology technologies have become commonplace. Our crisis scenario, which is set in 2030, imagines the mutation of widely-used synthetic fertilizer, FertiBac, to produce a toxin that is spreading into crops and waterways. See the full scenario <html><a href="https://static.igem.org/mediawiki/2012/9/95/Ucl2012-rathenau-finalscenario.pdf" title="Final Scenario" target="_blank">here</a></html>. <br />
<br />
Using this crisis scenario we provide a framework for the debate by setting out a possible future in which the fears raised by synthetic biology have come true – to discuss the ‘what now’ rather than the ‘what if’, to debate in practicalities rather than generalities.<br />
<br />
== The debate ==<br />
We are very pleased to have Annemiek Nelis of the Dutch Safety Board chairing our section of the Meeting of Young Minds. We will also have a panel of representatives from the UEA Norwich and Paris Bettencourt iGEM teams, the Oxford Conservative Association, GreenLeft Youth Organization, and National Federation of Young Farmer’s Clubs, amongst others. <br />
<br />
They will act as the ‘European Council of Synthetic Biology’, who will come together in a public hearing in the aftermath of the crisis. They will be responsible for deciding the best course of action to take and interrogating members of our team, as the scientist, manufacturer, marketer, and regulator of the failed technology. We will also hear questions and suggestions from the public, of which the best three suggestions will be announced on the iGEM wiki and the Rathenau website. <br />
<br />
== Position Paper ==<br />
As preparation for the debate, we were asked to write a 2500-word position paper. The paper, which is available <html><a href="https://static.igem.org/mediawiki/2012/b/b9/Ucl2012-rathenau-sectionsfinal.pdf" title="Final Sections" target="_blank">here</a></html>, is divided into three parts. The first part, ‘The synthetic biology present’, presents the current legal situation around deliberate release and transboundary movement of synthetic organisms. Part 2, ‘Next steps’, questions the need for changes to the existing legislation, as synthetic organisms move out of the laboratory towards industrial applications, to reflect the new challenges – real or perceived – posed by truly synthetic (as opposed to simply genetically modified) organisms. Part 3 ‘Into the future’, deals with the issues posed by our crisis scenario, looking at issues of liability and at how such a crisis might affect the future of synthetic biology.<br />
<br />
== We hope to see you there! ==<br />
For more information, please see https://2012.igem.org/Regions/Europe/MeetingofYoungMinds<br />
<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/cssTeam:University College London/css2012-10-26T16:14:50Z<p>PhilippBoeing: </p>
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<br />
iframe{<br />
border:none;<br />
}</div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-coverpicturemoym.jpgFile:Ucl2012-coverpicturemoym.jpg2012-10-26T16:14:44Z<p>PhilippBoeing: </p>
<hr />
<div></div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/HumanPractice/MOYMTeam:University College London/HumanPractice/MOYM2012-10-26T16:08:57Z<p>PhilippBoeing: /* Meeting Of Young Minds Debate */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=monkeyisland}}<br />
<br />
<html><div style="float:right"><iframe width="560" height="315" src="http://www.youtube.com/embed/GhjOQCk8E_k?list=UUQPIppvUH1aw9iqjdFEdp3g&amp;hl=en_US" frameborder="0" allowfullscreen></iframe></div></html><br />
= Meeting Of Young Minds Debate =<br />
<br />
== Meeting Of Young Minds ==<br />
At the beginning of June, the [http://www.rathenau.nl/en.html Rathenau Instituut] (a Dutch body which promotes the formation of political and public opinion on science and technology) put out a call for proposals for a debate, the Meeting of Young Minds, to be held on the Friday evening preceding the African-European jamboree. <br />
<br />
The first Meeting of Young Minds debate, which [http://www.youtube.com/watch?v=g5YPUFUayTo took place in 2011], pitted iGEM teams against representatives from Dutch youth political parties in a meeting of ‘future scientists and future politicians’ to discuss the ‘promises and perils of synthetic biology’. For Meeting of Young Minds 2012, iGEM teams were invivted to submit their own themes for debate. Our proposal ‘A synthetic biology future, a synthetic biology crisis’ was one of two themes selected.<br />
<br />
== Our proposal ==<br />
We decided that we wanted our proposal to have some link to our project, hoping that we might gain some new perspectives from the debate. At this point in the summer, we knew <i>what</i> our project was going to be, but not <i>how</i> it was going to work, so we came up with the idea of a debate broadly based around geoengineering – deliberate large-scale scientific projects to address or remedy the environmental impact of human activities. <br />
<br />
Early ideas included discussion on the ethics of syn-bio geoengineering and the rights and responsibilities relating to such large scale projects. However, our research quickly revealed that these topics had been already been well-publicized and thoroughly discussed. We decided to try and avoid the tired and abstract moral/ethical questions, and bring our debate into a more concrete setting by using a ‘crisis scenario’. <br />
<br />
== Crisis scenario ==<br />
The crisis scenario was inspired by the Rathenau Institute’s [http://www.rathenau.nl/themas/thema/project/synthetische-biologie/synbio-futures.html Synbio futures], a series of ‘techno-moral vignettes’ – short stories imagining possible futures in which synthetic biology technologies have become commonplace. Our crisis scenario, which is set in 2030, imagines the mutation of widely-used synthetic fertilizer, FertiBac, to produce a toxin that is spreading into crops and waterways. See the full scenario <html><a href="https://static.igem.org/mediawiki/2012/9/95/Ucl2012-rathenau-finalscenario.pdf" title="Final Scenario" target="_blank">here</a></html>. <br />
<br />
Using this crisis scenario we provide a framework for the debate by setting out a possible future in which the fears raised by synthetic biology have come true – to discuss the ‘what now’ rather than the ‘what if’, to debate in practicalities rather than generalities.<br />
<br />
== The debate ==<br />
We are very pleased to have Annemiek Nelis of the Dutch Safety Board chairing our section of the Meeting of Young Minds. We will also have a panel of representatives from the UEA Norwich and Paris Bettencourt iGEM teams, the Oxford Conservative Association, GreenLeft Youth Organization, and National Federation of Young Farmer’s Clubs, amongst others. <br />
<br />
They will act as the ‘European Council of Synthetic Biology’, who will come together in a public hearing in the aftermath of the crisis. They will be responsible for deciding the best course of action to take and interrogating members of our team, as the scientist, manufacturer, marketer, and regulator of the failed technology. We will also hear questions and suggestions from the public, of which the best three suggestions will be announced on the iGEM wiki and the Rathenau website. <br />
<br />
== Position Paper ==<br />
As preparation for the debate, we were asked to write a 2500-word position paper. The paper, which is available <html><a href="https://static.igem.org/mediawiki/2012/b/b9/Ucl2012-rathenau-sectionsfinal.pdf" title="Final Sections" target="_blank">here</a></html>, is divided into three parts. The first part, ‘The synthetic biology present’, presents the current legal situation around deliberate release and transboundary movement of synthetic organisms. Part 2, ‘Next steps’, questions the need for changes to the existing legislation, as synthetic organisms move out of the laboratory towards industrial applications, to reflect the new challenges – real or perceived – posed by truly synthetic (as opposed to simply genetically modified) organisms. Part 3 ‘Into the future’, deals with the issues posed by our crisis scenario, looking at issues of liability and at how such a crisis might affect the future of synthetic biology.<br />
<br />
== We hope to see you there! ==<br />
For more information, please see https://2012.igem.org/Regions/Europe/MeetingofYoungMinds<br />
<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/World_Championship_Jamboree/Schedule/Practice_sessionsWorld Championship Jamboree/Schedule/Practice sessions2012-10-24T10:09:56Z<p>PhilippBoeing: </p>
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<div class="main_item"> <!-- World Jamboree Schedule Goes Here--> <br />
<div class="title">Presentation Practice: Friday November 2nd 2012</div><br />
<br />
Use this sign-up sheet to sign up for a slot on Friday night (November 2nd) to practice your talk. Note that there will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. Be sure to bring necessary computer equipment with you, such as chargers and adapters, as these will not be provided.<br><br> <br />
<br />
There are a limited number of time slots available on a first-come first-serve basis so please only choose one slot. We cannot match the room that you will ultimately give your presentation in with the practice room. This should, however, give you a chance to practice your talk in a new environment. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time.<br><br><br />
<br />
Also, pre-registration will be available on Friday November 2nd<!-- beginning at <strong>1pm at Compton Lounge</strong>. Conference services will be on-site to pass out team registration boxes (see the <a href="https://2012.igem.org/World_Championship_Jamboree/Handbook">Jamboree Handbook</a>) -->. <br><br><br />
<br />
<strong>Note</strong>: Use the wiki edit button to add your team to the schedule (the markup is located at the bottom of the page). Room numbers and locations will be updated as soon as possible. Additional rooms may be added in the coming weeks.<br />
<br />
</div><br />
</td><br />
</tr><br />
</table><br />
</body><br />
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<br />
<html> <!--Practice Session Sign-Up Sheet - ADD YOUR TEAM NAME HERE!--><br />
<table class="calendar" align="center"><h2 class="date"><a name="Friday Practice">Friday, November 2</a></h2><br />
<thead><br />
<tr><br />
<th style="width:100px;">Time</th><br />
<th>RM 1 </th><br />
<th>RM 2 </th><br />
<th>RM 3 </th><br />
<th>RM 4 </th><br />
<th>RM 5 </th><br />
<th>RM 6 </th><br />
</tr><br />
</thead><br />
<tbody><br />
<tr class="even"><br />
<th>6:00p - 6:30p</th><br />
<td>Evry</td><br />
<td>SJTU-BioX-Shanghai</td><br />
<td>XMU-China</td><br />
<td>UT-Tokyo-Software</td><br />
<td>Macquarie-Australia</td><br />
<td>HKUST-Hong_Kong</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30p - 7:00p</th><br />
<td>Paris_Bettencourt</td><br />
<td>University College London</td><br />
<td>B3</td><br />
<td>B4</td><br />
<td>B5</td><br />
<td>B6</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00p - 7:30p</th><br />
<td>C1</td><br />
<td>UNITN-TRENTO</td><br />
<td>C3</td><br />
<td>C4</td><br />
<td>Bielefeld-Germany</td><br />
<td>C6</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30p - 8:00p</th><br />
<td>Buenos Aires</td><br />
<td>D2</td><br />
<td>D3</td><br />
<td>D4</td><br />
<td>D5</td><br />
<td>D6</td><br />
</tr><br />
<tr class="odd"><br />
<th>8:00p - 8:30p</th><br />
<td>ETH Zurich</td><br />
<td>Groningen</td><br />
<td>UNAM Genomics Mexico</td><br />
<td>E4</td><br />
<td>E5</td><br />
<td>E6</td><br />
</tr><br />
<tr class="even"><br />
<th>8:30p - 9:00p</th><br />
<td>F1</td><br />
<td>F2</td><br />
<td>F3</td><br />
<td>F4</td><br />
<td>F5</td><br />
<td>F6</td><br />
</tr><br />
<tr class="odd"><br />
<th>9:00p - 9:30p</th><br />
<td>G1</td><br />
<td>G2</td><br />
<td>G3</td><br />
<td>G4</td><br />
<td>G5</td><br />
<td>G6</td><br />
</tr><br />
<tr class="even"><br />
<th>9:30p - 10:00p</th><br />
<td>Queens_Canada</td><br />
<td>CINVESTAV-IPN-UNAM MX</td><br />
<td>Calgary</td><br />
<td>Penn</td><br />
<td>Berkeley</td><br />
<td>H6</td><br />
</tr><br />
</tbody><br />
</table><br />
</html></div>PhilippBoeinghttp://2012.igem.org/World_Championship_Jamboree/Schedule/Practice_sessionsWorld Championship Jamboree/Schedule/Practice sessions2012-10-24T10:09:25Z<p>PhilippBoeing: </p>
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<br />
<table align="center" style="width: 950px; margin-top:-10px;"><br />
<br><br />
<td colspan="3" valign="top"><br />
<div class="main_item"> <!-- World Jamboree Schedule Goes Here--> <br />
<div class="title">Presentation Practice: Friday November 2nd 2012</div><br />
<br />
Use this sign-up sheet to sign up for a slot on Friday night (November 2nd) to practice your talk. Note that there will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. Be sure to bring necessary computer equipment with you, such as chargers and adapters, as these will not be provided.<br><br> <br />
<br />
There are a limited number of time slots available on a first-come first-serve basis so please only choose one slot. We cannot match the room that you will ultimately give your presentation in with the practice room. This should, however, give you a chance to practice your talk in a new environment. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time.<br><br><br />
<br />
Also, pre-registration will be available on Friday November 2nd<!-- beginning at <strong>1pm at Compton Lounge</strong>. Conference services will be on-site to pass out team registration boxes (see the <a href="https://2012.igem.org/World_Championship_Jamboree/Handbook">Jamboree Handbook</a>) -->. <br><br><br />
<br />
<strong>Note</strong>: Use the wiki edit button to add your team to the schedule (the markup is located at the bottom of the page). Room numbers and locations will be updated as soon as possible. Additional rooms may be added in the coming weeks.<br />
<br />
</div><br />
</td><br />
</tr><br />
</table><br />
</body><br />
</html><br />
<br />
<html> <!--Practice Session Sign-Up Sheet - ADD YOUR TEAM NAME HERE!--><br />
<table class="calendar" align="center"><h2 class="date"><a name="Friday Practice">Friday, November 2</a></h2><br />
<thead><br />
<tr><br />
<th style="width:100px;">Time</th><br />
<th>RM 1 </th><br />
<th>RM 2 </th><br />
<th>RM 3 </th><br />
<th>RM 4 </th><br />
<th>RM 5 </th><br />
<th>RM 6 </th><br />
</tr><br />
</thead><br />
<tbody><br />
<tr class="even"><br />
<th>University College London</th><br />
<td>Evry</td><br />
<td>SJTU-BioX-Shanghai</td><br />
<td>XMU-China</td><br />
<td>UT-Tokyo-Software</td><br />
<td>Macquarie-Australia</td><br />
<td>HKUST-Hong_Kong</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30p - 7:00p</th><br />
<td>Paris_Bettencourt</td><br />
<td>B2</td><br />
<td>B3</td><br />
<td>B4</td><br />
<td>B5</td><br />
<td>B6</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00p - 7:30p</th><br />
<td>C1</td><br />
<td>UNITN-TRENTO</td><br />
<td>C3</td><br />
<td>C4</td><br />
<td>Bielefeld-Germany</td><br />
<td>C6</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30p - 8:00p</th><br />
<td>Buenos Aires</td><br />
<td>D2</td><br />
<td>D3</td><br />
<td>D4</td><br />
<td>D5</td><br />
<td>D6</td><br />
</tr><br />
<tr class="odd"><br />
<th>8:00p - 8:30p</th><br />
<td>ETH Zurich</td><br />
<td>Groningen</td><br />
<td>UNAM Genomics Mexico</td><br />
<td>E4</td><br />
<td>E5</td><br />
<td>E6</td><br />
</tr><br />
<tr class="even"><br />
<th>8:30p - 9:00p</th><br />
<td>F1</td><br />
<td>F2</td><br />
<td>F3</td><br />
<td>F4</td><br />
<td>F5</td><br />
<td>F6</td><br />
</tr><br />
<tr class="odd"><br />
<th>9:00p - 9:30p</th><br />
<td>G1</td><br />
<td>G2</td><br />
<td>G3</td><br />
<td>G4</td><br />
<td>G5</td><br />
<td>G6</td><br />
</tr><br />
<tr class="even"><br />
<th>9:30p - 10:00p</th><br />
<td>Queens_Canada</td><br />
<td>CINVESTAV-IPN-UNAM MX</td><br />
<td>Calgary</td><br />
<td>Penn</td><br />
<td>Berkeley</td><br />
<td>H6</td><br />
</tr><br />
</tbody><br />
</table><br />
</html></div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/MOYMTeam:University College London/MOYM2012-10-22T14:02:06Z<p>PhilippBoeing: Redirected page to Team:University College London/HumanPractice/MOYM</p>
<hr />
<div>#REDIRECT [[Team:University_College_London/HumanPractice/MOYM]]</div>PhilippBoeinghttp://2012.igem.org/IGEM_PublicityIGEM Publicity2012-10-17T09:46:04Z<p>PhilippBoeing: </p>
<hr />
<div>__NOTOC__<br />
<br />
<br />
<div style="color:#aaa; padding-bottom:20px;">(Members of the press, please see the [https://igem.org/Press_Kit | iGEM Press Kit])</div><br />
<br />
If you would like to share an article that was written about iGEM or your iGEM team, please link to it on this page. If you have multiple articles featuring your team, link to them all individually!<br />
<br />
Post your official team name, the title of your article, where it was featured, and provide a link to it. <br />
<br />
''Example'':<br> <br />
'''Team iGEM Headquarters''': ''Title of article'', Nature, [link]<br />
<br />
<br />
<span style="color:#1e90ff; font-size:150%">'''blogs </span><span style="color:#3cb371; font-size:150%">covering iGEM 2012'''</span><br><br />
'''Peking2012 iGEM''': ''Peking iGEM'', [http://page.renren.com/601444914] <br><br />
'''UANL Mexico''': ''Bio! iGEM Collection'', Blogspot, [http://biospot10.blogspot.de/2012/02/bio-about-igem.html]<br><br />
'''iGEM Valencia 2012''': ''Synth(ethic) Biology'', Wordpress, [http://igemvalencia2012.wordpress.com/]<br><br />
'''TU_Munich''': ''TUM iGEM 2012'', [http://tum-igem2012.blog.de/]<br><br />
<br />
<br><br />
<span style="color:#1e90ff; font-size:150%">'''video/radio</span><span style="color:#3cb371; font-size:150%"> about iGEM 2012'''</span><br><br />
'''TU_Munich''': LIVE radio interview, Kortex: TUM-Projektteam iGEM 2012 - Bier und synthetische Biologie, M94.5, soundcloud, [http://soundcloud.com/m945/kortex-tum-projektteam-igem]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices ---"Sowing Tomorrow Synthetic Biologists"'', [http://youtu.be/cd7m5POAwRY] <br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- Intro to Synthetic Biology'', [http://youtu.be/BpmCMeBqZuI]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- Research guideline of Synthetic Biology'', [http://youtu.be/gfNFW6LQ1SQ]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- iGEM HS-Division in a Nutshell'', [http://youtu.be/R3g0zVi0DgI]<br />
<br><br />
'''University College London''': ''#gemFM'', radioshow Mixcloud / Soundcloud, [https://2012.igem.org/Team:University_College_London/gemFM] <br><br />
'''Bielefeld-Germany''': ''LIVE radio interview'', Radio Hertz, [http://www.radiohertz.de/beta-site/2012/07/02/impuls-am-04-juli-2012/]<br><br />
'''SDU-Denmark''': ''Forsker i fritiden'', TV2 Fyn, [http://www.tv2fyn.dk/article/370281?autoplay=1&video_id=54463]<br><br />
'''University College London''':''London 'bio-hackers' catching the eye of professionals'', BBC News TV, [http://www.bbc.co.uk/news/uk-england-london-19964886]<br /><br />
<br><br />
<span style="color:#1e90ff; font-size:150%">'''news articles</span><span style="color:#3cb371; font-size:150%"> about iGEM 2012'''</span><br />
<br><br />
'''Peking2012 iGEM''': ''Lecture "Life as Machines --- Rational Design for Artificial Biological Systems"'', Beijing Teenager Science and Technology Club, [http://www.scitech-youth.org.cn/club_news/dt/4676.html] <br><br />
'''iGEM Leicester''', ''Google Campus hosts future gene-iuses'', University of Leicester press office, [http://www2.le.ac.uk/news/blog/2012/august/google-campus-hosts-future-gene-iuses]<br><br />
'''Lyon-INSA iGEM''': ''Ready, Set, Go for OSLI 2012 iGEM Teams'', Osli, [http://www.osli.ca/storybank/39/59/Ready-Set-Go-for-OSLI-2012-iGEM-Teams]<br />
<br />
====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
<br />
'''Peking2012 iGEM''': ''Designing the Scientific Cradle for Quantitative Biologists'', ACS Synth. Biol., [http://pubs.acs.org/doi/abs/10.1021/sb3000386]<br />
<br><br />
'''iGEM Teams Germany''', ''iGEM: Elf Teams tüfteln für die Biokonstrukteurs-WM'', biotechnologie.de, [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153792.html]<br><br />
'''iGEM Teams France''', ''Site iGEM France'', [https://sites.google.com/site/igemfrance/]<br><br />
<br />
'''Buenos Aires 2012 iGEM Team''', Synthetic Communities, <br />
[http://blogs.scientificamerican.com/lab-rat/2012/09/09/igem-buenos-aires-synthetic-bacterial-communities/]<br><br />
'''SDU-Denmark''': ''iGEM – Verdens største konkurrence indenfor syntetisk biologi vender tilbage til SDU'', Sund & Hed, [http://sundoghed.dk/7038/igem-verdens-storste-konkurrence-indenfor-syntetisk-biologi-vender-tilbage-til-sdu/?fb_comment_id=fbc_365648506787388_4738817_366895283329377]<br><br />
<br />
'''Waterloo 2012 iGEM Team''', Educational Podcast on SynBio by Virtual Researcher On Call (Canadian Educational Resource Organization) [http://sns.vroc.ca/p.jsp?i=17]<br><br />
<br />
====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
'''Arizona_State''': ''Students create low-cost biosensor to detect contaminated water in developing nations'',[https://asunews.asu.edu/20120906_waterbiosensor]<br/><br />
'''Potsdam-Bioware''': ''Hamsterzellen als Antikörperfabrik, biotechnologie.de'',[http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153816.html]<br/><br />
'''Valencia (UPV,UCV)''' : ''Seminar at the Institute of marine science (CSIC, Barcelona)'',[http://igemvalencia2012.wordpress.com/2012/09/11/seminar-in-barcelona/] <br/><br />
'''Valencia (UPV,UCV)''' : ''Interview with D. Justo Aznar, an expert in Bioethics, Wordpress'',[http://igemvalencia2012.wordpress.com/2012/08/29/interview-with-justo-aznar-lucena-m-d/] <br/><br />
'''Valencia (UPV,UCV)''' : ''What do you know about Synthetic Biology?, Wordpress'',[http://igemvalencia2012.wordpress.com/2012/09/07/what-do-you-know-about-synthetic-biology/] <br/><br />
'''TU Delft''' : ''Our team gets featured in the national news paper'',[https://static.igem.org/mediawiki/igem.org/c/c7/ZomerrepoNRC7aug.pdf] <br/><br />
'''TU Delft''' : '' Team members reaching out to the general public at The Night of Art and Science in Groningen to spread awareness on Synthetic Biology '', [http://www.youtube.com/watch?v=j9t7-Qnho7o&feature=share] <br/><br />
'''TU Delft''' : ''Licht aan de bacterie - Our team member Sietske explains how cool and fascinating Synthetic Biology is for LowLabs!! '', [http://www.youtube.com/watch?v=4q2Ol89ekz4&feature=youtu.be] <br/><br />
'''Valencia (UPV,UCV)''': ''Biohacking: Do it yourself!'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/21/biohacking-do-it-yourself/] <br/><br />
'''Valencia (UPV,UCV)''': ''Where will synthetic biology lead us?'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/13/a-life-of-its-own-where-will-synthetic-biology-lead-us-by-michael-specter/] <br/><br />
'''Valencia (UPV,UCV)''': ''iGEM: Why of all this'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/14/igem-the-why-of-all-this/] <br/><br />
'''University of Leicester''': ''Synthetic biology solution to polystyrene degradation'', Blogger, [http://uoleicesterigem2012.blogspot.co.uk/] <br/><br />
'''University College London''': ''Land Grab: Could Bioremediation Turn Pacific Garbage Patch Into Habitable Island?'', Good, [http://www.good.is/post/land-grab-could-bioremediation-turn-pacific-garbage-patch-into-habitable-island/] <br/><br />
'''University College London''': ''Synthetic Bacteria Could Turn Ocean Garbage into One Big Island'', Smithsonian, [http://blogs.smithsonianmag.com/smartnews/2012/07/synthetic-bacteria-could-turn-ocean-garbage-into-one-big-island/] <br/><br />
'''University College London''': ''Students synthesizing bacteria to create islands out of garbage'', DVICE, [http://dvice.com/archives/2012/07/students-synthe.php] <br/><br />
'''University College London''': ''Nanobots could turn 'Great Pacific Plastic Patch' into a floating island'', Wired, [http://www.wired.co.uk/news/archive/2012-07/18/nanobots-recycling-plastic] <br/><br />
'''University College London''': ''Synthetic Biology Speed Debate'', C-LAB, [http://c-lab.co.uk/events/synthetic-biology-speed-debate.html] <br/><br />
'''University College London''': ''From Pixels To DNA: The Next Frontier In Interaction Design'', Co.Design, [http://www.fastcodesign.com/1670825/from-pixels-to-dna-the-next-frontier-in-interaction-design#1]<br /><br />
'''University College London''': ''Biohacking, iGEM and the limits of citizen science'', Po Ve Sham, [http://povesham.wordpress.com/2012/10/05/biohacking-igem-and-the-limits-of-citizen-science/]<br /><br />
'''University College London''': ''Right or Risk? World's First Public BioBrick'', C-LAB, [http://c-lab.co.uk/events/right-or-risk-worlds-first-public-biobrick.html]<br /><br />
'''Queen's University''': ''Getting Biological over the Break'', Kingston This Week, [http://www.kingstonthisweek.com/2012/06/21/getting-biological-over-the-break] <br/><br />
'''Queen's University''': ''Dance like everybody's watching'', Kingston EMC, [http://www.emckingston.com/20120809/lifestyle/Dance+like+everybody%27s+watching] <br/><br />
'''Queen's University''': ''Dancing in the Lab'', The Queen's Journal, [http://queensjournal.ca/story/2012-09-11/news/dancing-lab/] <br/><br />
'''NRP UEA Norwich''': ''UEA Students Compete For Top Biology Prize'', UEA Press Release, [http://www.uea.ac.uk/mac/comm/media/press/2012/August/igem-competition-uea]<br><br />
'''NRP UEA Norwich''': ''Interview With Students From The UEA Talking About Synthetic Biology (Show 23)'', The Farming Show: STAR Radio, [http://www.star107.co.uk/farming-show.php]<br><br />
'''University of St Andrews''': "Streets of London: paved with platinum" [http://www.st-andrews.ac.uk/news/archive/2012/Title,89409,en.html] <br><br />
'''TU_Munich''': ''Technische Universität München: Brauhefe für leckeres und gesundes Bier'', biotechnologie.de, [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153818.html]<br><br />
'''TU_Munich''': ''TUMstudis: baker's yeast is synthetically converted'', TUMstudinews, [http://portal.mytum.de/ccc/newsletter/students/2012_03/02]<br><br />
'''TU_Munich''': ''Die Wunderhefe'', jetzt.de sueddeutsche zeitung, [http://jetzt.sueddeutsche.de/texte/anzeigen/544866/4/1#texttitel]<br><br />
'''TU_Munich''': ''Widerstand gegen die "Grüne Gentechnik" wächst weiter'', merkur, [http://www.merkur-online.de/lokales/freising/widerstand-gegen-gruene-gentechnik-waechst-weiter-2510648.html]<br><br />
'''Tübingen''': ''Tübinger Studenten nehmen an internationalem Forscherwettbewerb teil'', Schwäbisches Tagblatt, [http://www.tagblatt.de/Home/nachrichten/hochschule_artikel,-Tuebinger-Studenten-nehmen-an-internationalem-Forscherwettbewerb-teil-_arid,184906.html]<br><br />
'''Bielefeld-Germany''': ''Studierende der Universität Bielefeld nehmen am iGEM-Wettbewerb teil'', Universität Bielefeld, [http://ekvv.uni-bielefeld.de/blog/pressemitteilungen/entry/östrogen_aus_trinkwasser_entfernen_nr]<br><br />
'''Bielefeld-Germany''': ''Östrogen aus Trinkwasser entfernen'', Press Release, [http://www.uni-protokolle.de/nachrichten/id/240229/][http://www.laborpraxis.vogel.de/forschung-und-entwicklung/analytik/articles/369125/][http://www.bio.nrw.de/nachrichten][http://idw-online.de/pages/de/news484982][http://www.eurekalert.org/pub_releases/2012-06/uob-ref062512.php]<br><br />
'''Bielefeld-Germany''': ''Turkey tail tree fungus could filter oestrogen from water'', Wired, [http://www.wired.co.uk/news/archive/2012-06/25/tree-fungus-oestrogen-filter]<br><br />
'''Bielefeld-Germany''': ''Biological filter to remove estrogens from waste water and drinking water'', News Medical Australia, [http://www.news-medical.net/news/20120626/Biological-filter-to-remove-estrogens-from-waste-water-and-drinking-water.aspx]<br><br />
'''Bielefeld-Germany''': ''Das Wasser wird weiblich'', Neue Westfälische, [https://2012.igem.org/Team:Bielefeld-Germany/Homeleft]<br><br />
'''Bielefeld-Germany''': ''Arzneimittel in Gewässern minimieren'', Neue Züricher Zeitung, [http://www.nzz.ch/wissen/wissenschaft/arzneimittel-in-gewaessern-minimieren-1.17368069]<br><br />
'''USP-UNESP-Brazil''': ''Brazilian Science Dreams Come Alive'', RocketHub Blog, [http://blog.rockethub.com/brazilian-science-dreams-come-alive]<br><br />
'''USP-UNESP-Brazil''': ''USP participa pela 1ª vez de campeonato de biologia sintética'', Jornal do Campus, [http://www.jornaldocampus.usp.br/index.php/2012/06/usp-participa-pela-1a-vez-de-campeonato-de-biologia-sintetica/]<br><br />
'''USP-UNESP-Brazil''': ''Cientistas da USP conseguem financiar projeto com vaquinha virtual'', Folha de S.Paulo, [http://www1.folha.uol.com.br/ciencia/1097202-cientistas-da-usp-conseguem-financiar-projeto-com-vaquinha-virtual.shtml]<br><br />
'''EPF-Lausanne''': ''Fabriquer des médicaments en allumant la lumière?'' (French), Flash EPFL, [http://actualites.epfl.ch/newspaper-edition?np_id=151] [https://static.igem.org/mediawiki/2012/4/42/Team-EPF-Lausanne_Flash_article_about_us.pdf]<br><br />
'''Lyon INSA iGEM''' : ''L’INSA de Lyon vise l’or à Amsterdam'' , En vue INSA Lyon, [http://envue.insa-lyon.fr/2012avril/art2a_0412.php]<br><br />
'''Trieste''': ''Human practices - SynBio for everyone (Italian)'', Il Tascapane, [http://tascapane.blogspot.se/2012/08/la-biologia-sintetica.html]<br><br />
'''Trieste''': ''Human practices - SynBio and medicine (Italian)'', Il Tascapane,[http://tascapane.blogspot.it/2012/09/la-biologia-sintetica-approda-in.html]<br><br />
'''Trieste''': '' Un'idea per proteggere l'intestino (Italian)'', ICGEB, [http://www.icgeb.org/notizie-in-italiano/items/squadra-igem-2012-un-idee-per-proteggere-lintestino.html]<br><br />
'''Lyon-INSA''':''M. concourt au titre mondial de biologie de synthèse'', Républicain Lorrain [https://docs.google.com/file/d/0BzhWvySCD_KzWmJTYjBYWHBCUnM/edit]<br><br />
'''Lyon-INSA''' : ''L'Insa de Lyon vise l'or à Amsterdam'', Insa de Lyon [http://www.insa-lyon.fr/fr/concours-igem-2012]<br><br />
'''UANL_Mty-Mexico''': ''Túnel de la Biología Sintética (Synthetic Biology Tunnel), Televisa'', Monterrey,[http://www.televisaregional.com/monterrey/video/169491536.html]<br/><br />
'''Wageningen_UR''': ''The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts'', Journal of Biological Engineering,[http://www.jbioleng.org/content/6/1/14/abstract]<br/><br />
'''SDU-Denmark''': ''Fighting Obesity at the SDU in Odense'', SIJ, by Eliana van de Craats, [http://community.ejc.net/forum/topic/show?id=2345725%3ATopic%3A89026&xgs=1&xg_source=msg_share_topic]<br/><br />
'''Georgia_Tech''': ''<br />
Georgia Tech workshop well received: Lambert students embrace engineering'', ForsythNews.com, [http://www.forsythnews.com/m/section/3/article/14721/. ]<br/><br />
'''Cornell''': ''Using electroactive bacteria, students design toxin sensor'', Cornell Chronicle, [http://www.news.cornell.edu/stories/Oct12/oilSands.html]</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/HumanPractice/DIYbio/WorkshopsTeam:University College London/HumanPractice/DIYbio/Workshops2012-10-15T13:11:11Z<p>PhilippBoeing: /* =Write-up and results analysis = */</p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=hackspace}}<br />
= DIYbio =<br />
{{:Team:University_College_London/templates/diybiomenu}}<br />
== Public biobrick:[http://partsregistry.org/Part:BBa_K729016 BBa_K729016: Antifreeze protein, type I from Oceanibulbus indolifex HEL-45] ==<br />
Sequence: http://www.ebi.ac.uk/ena/data/view/EDQ05862<br />
<br />
== Planning ==<br />
Prior to the workshops, we visited the Hackspace on several occasions to prepare for the wetlab sessions. Our visits included giving a short introduction to synthetic biology and our project, a large health and safety discussion, and a primer design workshop. From our early interactions with the citizen scientists, we found that they had previously performed many PCRs and gels. Some of these had been successful. However they have not attempted cloning before, due to the of lack laboratory license and UK regulations. What they wanted from this collaboration was to visit our lab to see how we conduct experiments in an academic institution. They also wanted to perform all the steps in the cloning process. Therefore in the UCL workshop schedule, we attempted to fit all the cloning experiments in three days. <br />
<br />
<strong>Selecting genes to PCR from Oceanibulbus Indolifex</strong><br />
<br />
Which genes to PCR? The Biohackers researched a list of genes from the [http://www.xbase.ac.uk/genome/oceanibulbus-indolifex-hel-45/NZ_ABID01000001/features <em> Oceanibulbus Indolifex</em> genome database] and selected genes which they thought would be useful when transformed into ''E.coli''. There were a number of potential candidates, including metal binding proteins, arsenic reductase, mercuric reductase and antifreeze. The list was then shortened to antifreeze and mercuric reductase. Antifreeze was chosen because a relatively easy assay was found to characterize the gene, while mercuric reductase was chosen due to its useful ability to detoxify mercury.<br />
<br />
<strong>Hackspace Workshop 29th-31st</strong><br />
<br />
<html><a href="https://static.igem.org/mediawiki/2012/b/b7/Ucl2012-Hackspace_Schedule_2930th_aug.pdf" target="_blank">Hackspace Wetlab Workshop schedule</a></html><br />
<br />
In the Hackspace, we worked with the Biohackers on making competent <em>E.coli</em> (W3110) cells in the lab. This was an exciting experience, as we had to make a DIY incubator and shaker ourselves. We had help from many people in the Hackspace from sawing wood to circuit building. For the PCR, we autoclaved our boxes of tubes and tips using their makeshift autoclave (a pressure cooker) and then the boxes were put in the oven to dry. <br />
{{:Team:University_College_London/fbphotosbytudelft|album=348430135241378|count=6}}<br />
<br />
<strong>UCL Workshops 3rd-5th September</strong><br />
<br />
<html><a href="https://static.igem.org/mediawiki/2012/2/24/Ucl2012-UCL_Laboratory_Schedule.pdf" target="_blank">Original UCL Wetlab Workshop schedule</a></html><br />
<br />
In the beginning of September, we held a three day workshop in UCL's teaching laboratory for the citizen scientists. We had an ambitious plan for the workshops, from genomic DNA extraction to ligation and transformation. This was a packed schedule. However, after day one, we quickly realised that it would be a much better learning experience for the Biohackers if the pace was slowed down. The amount of practical experience of each citizen scientists also varied. We allowed much longer time intervals between the practicals to answer the Biohackers' questions and to show them professional techniques we use in the laboratory.<br />
<br />
Unfortunately, the first genomic DNA extraction was unsuccessful and some planned workshop items were delayed. However, the participants remarked how exciting and insightful they found the trouble-shooting that ensued.<br />
{{:Team:University_College_London/fbphotosbytudelft|album=350135541737504|count=6}}<br />
<br />
<strong>Post-Workshops</strong><br />
<br />
Some of the work was done after the workshops. Now that the Biohackers have the experience of working in an academic laboratory, they pay more attention to reducing contamination, and health & safety, particularly with regards to Ethidium Bromide.<br />
<br />
==Write-up and results analysis ==<br />
<br />
One of our aims for the collaboration was to encourage note-taking to track errors and improve the accuracy of the experiments ran in the London Hackspace. The Biohackers kept their own lab-books for the duration of the workshops and uploaded this data to their wiki.<br />
<br />
'''Primers, protocols and results can be found on the [http://wiki.london.hackspace.org.uk/view/Public_Biobrick#Visualisation_and_interpretation London Hackspace Wiki]'''<br />
<br />
<em><strong>"[Some of the skills I developed during the workshops included] documenting reactions, reaction mixes, tables of reagents, etc.<br />
interpreting… using controls, positive, negative controls on tests - so you know what you did wrong." </strong><br />
- Reflections of a Biohacker on the workshops</em><br />
<br />
== Result: Analytical Digest of the Public Biobrick ==<br />
[[File:Analytical_digest_public_biobrick.jpg]]<br />
<br />
<div class="">'''''</div> <br />
Five colonies were picked from the ligation plates. After inoculation in LB + chloramphenicol overnight, these were minipreped and analytical digest was performed. All samples in lanes labelled one were digested once with Eco RI and samples labelled two are digested with EcoRI and Pst I. Colonies C and D clearly have the antifreeze insert. Lanes C2 and D2 show bands around 2000bp for the CMP plasmid backbone and around 400bp for the 435bp antifreeze region amplified by the primers. Therefore we have the biobrick!<br />
<br />
== Safety and regulations ==<br />
To comply with UK/EU regulations on GMOs, we conducted transformation of our ligated plasmid into W3110 ''E.coli'' cells at UCL. <br />
<br />
<strong>Making Competent Cells</strong><br />
<br />
<html><a href="https://static.igem.org/mediawiki/2012/4/46/Ucl2012-diybio-competentcells.pdf" <br />
target="_blank">Legality of Making Competent cell at London Hackspace Document </a></html><br />
<br />
We attempted to make <em>''E. coli''</em> W3110 cells chemically competent at Hackspace. After long discussion and checking with relevant documents, we concluded that making these cells does not constitute genetic modification and therefore there is no legal restrictions on the creation of this competent strain.<br />
<br />
We discussed health and safety issues at hackspace prior to starting any practical wetlab activities. It was important to hear what safety guidelines are normally followed at the hackspace and also brainstorm why it is important to follow these guidlines in the laboratory. Particularly, we focused on the potential risks of the reagents that are going to be used.<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/DIYbioTeam:University College London/DIYbio2012-10-15T13:10:48Z<p>PhilippBoeing: Redirected page to Team:University College London/HumanPractice/DIYbio</p>
<hr />
<div>#REDIRECT [[Team:University_College_London/HumanPractice/DIYbio]]</div>PhilippBoeinghttp://2012.igem.org/Team:University_College_London/AttributionsTeam:University College London/Attributions2012-10-10T11:04:56Z<p>PhilippBoeing: </p>
<hr />
<div>{{:Team:University_College_London/templates/head|coverpicture=portico}}<br />
<br />
All work has been conducted by the team except for contributions mentioned here. We would like to thank the following people and organisations for their assistance:<br />
<br />
Supervisors, Darren Nesbeth and Eli Keshavarz-Moore - requested funding from UCL Biochemical Engineering Department and provided outstanding support<br />
<br />
Yanika Borg, Erick Ramos and Alex Templar - our instructors <br />
<br />
Howard Boland from <html><a href="http://www.c-lab.co.uk" target="_blank">C-LAB</a></html>, our brilliant friend and artistic advisor. <br />
<br />
Dr Paola Gomez-Perira, Researcher in Ocean Biogeochemistry and Ecosystems at Southampton University, advised Bouran on Marine Chassis and Bethan on Sediment Sampling and Experimental Design.<br />
<br />
Ben Mackrow, Research Associate in Algal Biotechnology at UCL, helped Bouran with Gibson Assembly and Construction feedback. <br />
<br />
Jason Gardiner and Douglas Ridgway, from the Alberta iGEM team, gave Bouran feedback on the Alberta BioBricks.<br />
<br />
Sean Ward and Markus Gershater, from Synthace, advised Bouran on Experimental Design and for help with electroporation. <br />
<br />
Prof. Mike Hoare, gave James and Leonard advice on creating a small scale shear device.<br />
<br />
Stuart Duffy, assisted in the creating and operation of the small scale shear device.<br />
<br />
<br />
== Modelling ==<br />
<br />
Dr David Rowley for help with the initial version of our degradation model<br />
<br />
Dr Andrew Martin for his assistance on running PERL scripts<br />
<br />
Miriam Goldstein for her help in finding data for the ocean model<br />
<br />
Stephen Fawcett for his help with ocean physics<br />
<br />
Julie Masura & Peter Ryan for their data on microplastic distribution<br />
<br />
Dr Chris Barnes for his tireless support with all aspects of our modelling<br />
<br />
<br />
== Human Practices ==<br />
<br />
Dr Muki Hacklay, advised Philipp, Bethan and Yeping on the development of our DIYbio - citizen science collaboration.<br />
<br />
Dr Steve Cross, <br />
<br />
Kate Oliver, from UCL Faculty of Engineering, advised Philipp and Bethan on publishing events to the public, finding resources within UCL and lent us tables for our Public BioBrick Exhibition. <br />
<br />
Kimberley Freeman and Hillary Jackson, from the UCL Department of Public Engagement, for their support and feedback for our Beacon Bursary application.<br />
<br />
Virgil Rerimassie & Dirk Stemerding, from the Rathenau Instituut in Den Haag, supported Erin with our application and development of our proposal for the Meeting of Young Minds debate, held by the Rathenau Institute. <br />
<br />
Claire Marris & Maggie Leggett for agreeing to provide support with our position paper for the Meeting of Young Minds debate<br />
<br />
Alan Evans at the National Oceanography Centre, Southampton, for his kind assistance on UNCLOS and international ocean law<br />
<br />
Mike Hughes, from Guerrilla Science, advised Yeping and Martina on public outreach and gave feedback on our DIYbio workshops.<br />
<br />
William Beaufoy, Simon Rose, Nicolas FitzRoy Dale, Andrew Cousins, Taylor Burcs and Joel Winston from the London Biohackers<br />
<br />
== Miscellaneous ==<br />
<br />
Prof Andrea Sella for providing us with dry ice and moral support<br />
<br />
Brian O'Sullivan & Gareth Mannall thanks to whom we now have a clean microwave<br />
<br />
Paula Thomas & Hayley Powell for their help getting rooms and equipment<br />
<br />
{{:Team:University_College_London/templates/foot}}</div>PhilippBoeinghttp://2012.igem.org/IGEM_PublicityIGEM Publicity2012-10-10T10:26:55Z<p>PhilippBoeing: /* team specific */</p>
<hr />
<div>__NOTOC__<br />
<br />
<br />
<div style="color:#aaa; padding-bottom:20px;">(Members of the press, please see the [https://igem.org/Press_Kit | iGEM Press Kit])</div><br />
<br />
If you would like to share an article that was written about iGEM or your iGEM team, please link to it on this page. If you have multiple articles featuring your team, link to them all individually!<br />
<br />
Post your official team name, the title of your article, where it was featured, and provide a link to it. <br />
<br />
''Example'':<br> <br />
'''Team iGEM Headquarters''': ''Title of article'', Nature, [link]<br />
<br />
<br />
<span style="color:#1e90ff; font-size:150%">'''blogs </span><span style="color:#3cb371; font-size:150%">covering iGEM 2012'''</span><br><br />
'''Peking2012 iGEM''': ''Peking iGEM'', [http://page.renren.com/601444914] <br><br />
'''UANL Mexico''': ''Bio! iGEM Collection'', Blogspot, [http://biospot10.blogspot.de/2012/02/bio-about-igem.html]<br><br />
'''iGEM Valencia 2012''': ''Synth(ethic) Biology'', Wordpress, [http://igemvalencia2012.wordpress.com/]<br><br />
'''TU_Munich''': ''TUM iGEM 2012'', [http://tum-igem2012.blog.de/]<br><br />
<br />
<br><br />
<span style="color:#1e90ff; font-size:150%">'''video/radio</span><span style="color:#3cb371; font-size:150%"> about iGEM 2012'''</span><br><br />
'''TU_Munich''': LIVE radio interview, Kortex: TUM-Projektteam iGEM 2012 - Bier und synthetische Biologie, M94.5, soundcloud, [http://soundcloud.com/m945/kortex-tum-projektteam-igem]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices ---"Sowing Tomorrow Synthetic Biologists"'', [http://youtu.be/cd7m5POAwRY] <br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- Intro to Synthetic Biology'', [http://youtu.be/BpmCMeBqZuI]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- Research guideline of Synthetic Biology'', [http://youtu.be/gfNFW6LQ1SQ]<br />
<br><br />
'''Peking2012 iGEM''': ''Human Practices --- Series Lectures --- iGEM HS-Division in a Nutshell'', [http://youtu.be/R3g0zVi0DgI]<br />
<br><br />
'''University College London''': ''#gemFM'', radioshow Mixcloud / Soundcloud, [https://2012.igem.org/Team:University_College_London/gemFM] <br><br />
'''Bielefeld-Germany''': ''LIVE radio interview'', Radio Hertz, [http://www.radiohertz.de/beta-site/2012/07/02/impuls-am-04-juli-2012/]<br><br />
'''SDU-Denmark''': ''Forsker i fritiden'', TV2 Fyn, [http://www.tv2fyn.dk/article/370281?autoplay=1&video_id=54463]<br><br />
<br />
<br><br />
<span style="color:#1e90ff; font-size:150%">'''news articles</span><span style="color:#3cb371; font-size:150%"> about iGEM 2012'''</span><br />
<br><br />
'''Peking2012 iGEM''': ''Lecture "Life as Machines --- Rational Design for Artificial Biological Systems"'', Beijing Teenager Science and Technology Club, [http://www.scitech-youth.org.cn/club_news/dt/4676.html] <br><br />
'''iGEM Leicester''', ''Google Campus hosts future gene-iuses'', University of Leicester press office, [http://www2.le.ac.uk/news/blog/2012/august/google-campus-hosts-future-gene-iuses]<br><br />
'''Lyon-INSA iGEM''': ''Ready, Set, Go for OSLI 2012 iGEM Teams'', Osli, [http://www.osli.ca/storybank/39/59/Ready-Set-Go-for-OSLI-2012-iGEM-Teams]<br />
<br />
====<font size=4><font color=dodgerblue>'''general'''</font></font>====<br />
<br />
'''Peking2012 iGEM''': ''Designing the Scientific Cradle for Quantitative Biologists'', ACS Synth. Biol., [http://pubs.acs.org/doi/abs/10.1021/sb3000386]<br />
<br><br />
'''iGEM Teams Germany''', ''iGEM: Elf Teams tüfteln für die Biokonstrukteurs-WM'', biotechnologie.de, [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153792.html]<br><br />
'''iGEM Teams France''', ''Site iGEM France'', [https://sites.google.com/site/igemfrance/]<br><br />
<br />
'''Buenos Aires 2012 iGEM Team''', Synthetic Communities, <br />
[http://blogs.scientificamerican.com/lab-rat/2012/09/09/igem-buenos-aires-synthetic-bacterial-communities/]<br><br />
'''SDU-Denmark''': ''iGEM – Verdens største konkurrence indenfor syntetisk biologi vender tilbage til SDU'', Sund & Hed, [http://sundoghed.dk/7038/igem-verdens-storste-konkurrence-indenfor-syntetisk-biologi-vender-tilbage-til-sdu/?fb_comment_id=fbc_365648506787388_4738817_366895283329377]<br><br />
<br />
====<font size=4><font color=dodgerblue>'''team specific'''</font></font>====<br />
'''Arizona_State''': ''Students create low-cost biosensor to detect contaminated water in developing nations'',[https://asunews.asu.edu/20120906_waterbiosensor]<br/><br />
'''Potsdam-Bioware''': ''Hamsterzellen als Antikörperfabrik, biotechnologie.de'',[http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153816.html]<br/><br />
'''Valencia (UPV,UCV)''' : ''Seminar at the Institute of marine science (CSIC, Barcelona)'',[http://igemvalencia2012.wordpress.com/2012/09/11/seminar-in-barcelona/] <br/><br />
'''Valencia (UPV,UCV)''' : ''Interview with D. Justo Aznar, an expert in Bioethics, Wordpress'',[http://igemvalencia2012.wordpress.com/2012/08/29/interview-with-justo-aznar-lucena-m-d/] <br/><br />
'''Valencia (UPV,UCV)''' : ''What do you know about Synthetic Biology?, Wordpress'',[http://igemvalencia2012.wordpress.com/2012/09/07/what-do-you-know-about-synthetic-biology/] <br/><br />
'''TU Delft''' : ''Our team gets featured in the national news paper'',[https://static.igem.org/mediawiki/igem.org/c/c7/ZomerrepoNRC7aug.pdf] <br/><br />
'''TU Delft''' : '' Team members reaching out to the general public at The Night of Art and Science in Groningen to spread awareness on Synthetic Biology '', [http://www.youtube.com/watch?v=j9t7-Qnho7o&feature=share] <br/><br />
'''TU Delft''' : ''Licht aan de bacterie - Our team member Sietske explains how cool and fascinating Synthetic Biology is for LowLabs!! '', [http://www.youtube.com/watch?v=4q2Ol89ekz4&feature=youtu.be] <br/><br />
'''Valencia (UPV,UCV)''': ''Biohacking: Do it yourself!'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/21/biohacking-do-it-yourself/] <br/><br />
'''Valencia (UPV,UCV)''': ''Where will synthetic biology lead us?'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/13/a-life-of-its-own-where-will-synthetic-biology-lead-us-by-michael-specter/] <br/><br />
'''Valencia (UPV,UCV)''': ''iGEM: Why of all this'', Wordpress, [http://igemvalencia2012.wordpress.com/2012/08/14/igem-the-why-of-all-this/] <br/><br />
'''University of Leicester''': ''Synthetic biology solution to polystyrene degradation'', Blogger, [http://uoleicesterigem2012.blogspot.co.uk/] <br/><br />
'''University College London''': ''Land Grab: Could Bioremediation Turn Pacific Garbage Patch Into Habitable Island?'', Good, [http://www.good.is/post/land-grab-could-bioremediation-turn-pacific-garbage-patch-into-habitable-island/] <br/><br />
'''University College London''': ''Synthetic Bacteria Could Turn Ocean Garbage into One Big Island'', Smithsonian, [http://blogs.smithsonianmag.com/smartnews/2012/07/synthetic-bacteria-could-turn-ocean-garbage-into-one-big-island/] <br/><br />
'''University College London''': ''Students synthesizing bacteria to create islands out of garbage'', DVICE, [http://dvice.com/archives/2012/07/students-synthe.php] <br/><br />
'''University College London''': ''Nanobots could turn 'Great Pacific Plastic Patch' into a floating island'', Wired, [http://www.wired.co.uk/news/archive/2012-07/18/nanobots-recycling-plastic] <br/><br />
'''University College London''': ''Synthetic Biology Speed Debate'', C-LAB, [http://c-lab.co.uk/events/synthetic-biology-speed-debate.html] <br/><br />
'''University College London''': ''From Pixels To DNA: The Next Frontier In Interaction Design'', Co.Design, [http://www.fastcodesign.com/1670825/from-pixels-to-dna-the-next-frontier-in-interaction-design#1]<br /><br />
'''University College London''': ''Biohacking, iGEM and the limits of citizen science'', Po Ve Sham, [http://povesham.wordpress.com/2012/10/05/biohacking-igem-and-the-limits-of-citizen-science/]<br /><br />
'''University College London''': ''Right or Risk? World's First Public BioBrick'', C-LAB, [http://c-lab.co.uk/events/right-or-risk-worlds-first-public-biobrick.html]<br /><br />
'''Queen's University''': ''Getting Biological over the Break'', Kingston This Week, [http://www.kingstonthisweek.com/2012/06/21/getting-biological-over-the-break] <br/><br />
'''Queen's University''': ''Dance like everybody's watching'', Kingston EMC, [http://www.emckingston.com/20120809/lifestyle/Dance+like+everybody%27s+watching] <br/><br />
'''Queen's University''': ''Dancing in the Lab'', The Queen's Journal, [http://queensjournal.ca/story/2012-09-11/news/dancing-lab/] <br/><br />
'''NRP UEA Norwich''': ''UEA Students Compete For Top Biology Prize'', UEA Press Release, [http://www.uea.ac.uk/mac/comm/media/press/2012/August/igem-competition-uea]<br><br />
'''NRP UEA Norwich''': ''Interview With Students From The UEA Talking About Synthetic Biology (Show 23)'', The Farming Show: STAR Radio, [http://www.star107.co.uk/farming-show.php]<br><br />
'''University of St Andrews''': "Streets of London: paved with platinum" [http://www.st-andrews.ac.uk/news/archive/2012/Title,89409,en.html] <br><br />
'''TU_Munich''': ''Technische Universität München: Brauhefe für leckeres und gesundes Bier'', biotechnologie.de, [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=153818.html]<br><br />
'''TU_Munich''': ''TUMstudis: baker's yeast is synthetically converted'', TUMstudinews, [http://portal.mytum.de/ccc/newsletter/students/2012_03/02]<br><br />
'''TU_Munich''': ''Die Wunderhefe'', jetzt.de sueddeutsche zeitung, [http://jetzt.sueddeutsche.de/texte/anzeigen/544866/4/1#texttitel]<br><br />
'''TU_Munich''': ''Widerstand gegen die "Grüne Gentechnik" wächst weiter'', merkur, [http://www.merkur-online.de/lokales/freising/widerstand-gegen-gruene-gentechnik-waechst-weiter-2510648.html]<br><br />
'''Tübingen''': ''Tübinger Studenten nehmen an internationalem Forscherwettbewerb teil'', Schwäbisches Tagblatt, [http://www.tagblatt.de/Home/nachrichten/hochschule_artikel,-Tuebinger-Studenten-nehmen-an-internationalem-Forscherwettbewerb-teil-_arid,184906.html]<br><br />
'''Bielefeld-Germany''': ''Studierende der Universität Bielefeld nehmen am iGEM-Wettbewerb teil'', Universität Bielefeld, [http://ekvv.uni-bielefeld.de/blog/pressemitteilungen/entry/östrogen_aus_trinkwasser_entfernen_nr]<br><br />
'''Bielefeld-Germany''': ''Östrogen aus Trinkwasser entfernen'', Press Release, [http://www.uni-protokolle.de/nachrichten/id/240229/][http://www.laborpraxis.vogel.de/forschung-und-entwicklung/analytik/articles/369125/][http://www.bio.nrw.de/nachrichten][http://idw-online.de/pages/de/news484982][http://www.eurekalert.org/pub_releases/2012-06/uob-ref062512.php]<br><br />
'''Bielefeld-Germany''': ''Turkey tail tree fungus could filter oestrogen from water'', Wired, [http://www.wired.co.uk/news/archive/2012-06/25/tree-fungus-oestrogen-filter]<br><br />
'''Bielefeld-Germany''': ''Biological filter to remove estrogens from waste water and drinking water'', News Medical Australia, [http://www.news-medical.net/news/20120626/Biological-filter-to-remove-estrogens-from-waste-water-and-drinking-water.aspx]<br><br />
'''Bielefeld-Germany''': ''Das Wasser wird weiblich'', Neue Westfälische, [https://2012.igem.org/Team:Bielefeld-Germany/Homeleft]<br><br />
'''Bielefeld-Germany''': ''Arzneimittel in Gewässern minimieren'', Neue Züricher Zeitung, [http://www.nzz.ch/wissen/wissenschaft/arzneimittel-in-gewaessern-minimieren-1.17368069]<br><br />
'''USP-UNESP-Brazil''': ''Brazilian Science Dreams Come Alive'', RocketHub Blog, [http://blog.rockethub.com/brazilian-science-dreams-come-alive]<br><br />
'''USP-UNESP-Brazil''': ''USP participa pela 1ª vez de campeonato de biologia sintética'', Jornal do Campus, [http://www.jornaldocampus.usp.br/index.php/2012/06/usp-participa-pela-1a-vez-de-campeonato-de-biologia-sintetica/]<br><br />
'''USP-UNESP-Brazil''': ''Cientistas da USP conseguem financiar projeto com vaquinha virtual'', Folha de S.Paulo, [http://www1.folha.uol.com.br/ciencia/1097202-cientistas-da-usp-conseguem-financiar-projeto-com-vaquinha-virtual.shtml]<br><br />
'''EPF-Lausanne''': ''Fabriquer des médicaments en allumant la lumière?'' (French), Flash EPFL, [http://actualites.epfl.ch/newspaper-edition?np_id=151] [https://static.igem.org/mediawiki/2012/4/42/Team-EPF-Lausanne_Flash_article_about_us.pdf]<br><br />
'''Lyon INSA iGEM''' : ''L’INSA de Lyon vise l’or à Amsterdam'' , En vue INSA Lyon, [http://envue.insa-lyon.fr/2012avril/art2a_0412.php]<br><br />
'''Trieste''': ''Human practices - SynBio for everyone (Italian)'', Il Tascapane, [http://tascapane.blogspot.se/2012/08/la-biologia-sintetica.html]<br><br />
'''Trieste''': ''Human practices - SynBio and medicine (Italian)'', Il Tascapane,[http://tascapane.blogspot.it/2012/09/la-biologia-sintetica-approda-in.html]<br><br />
'''Trieste''': '' Un'idea per proteggere l'intestino (Italian)'', ICGEB, [http://www.icgeb.org/notizie-in-italiano/items/squadra-igem-2012-un-idee-per-proteggere-lintestino.html]<br><br />
'''Lyon-INSA''':''M. concourt au titre mondial de biologie de synthèse'', Républicain Lorrain [https://docs.google.com/file/d/0BzhWvySCD_KzWmJTYjBYWHBCUnM/edit]<br><br />
'''Lyon-INSA''' : ''L'Insa de Lyon vise l'or à Amsterdam'', Insa de Lyon [http://www.insa-lyon.fr/fr/concours-igem-2012]<br><br />
'''UANL_Mty-Mexico''': ''Túnel de la Biología Sintética (Synthetic Biology Tunnel), Televisa'', Monterrey,[http://www.televisaregional.com/monterrey/video/169491536.html]<br/><br />
'''Wageningen_UR''': ''The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts'', Journal of Biological Engineering,[http://www.jbioleng.org/content/6/1/14/abstract]<br/><br />
'''SDU-Denmark''': ''Fighting Obesity at the SDU in Odense'', SIJ, by Eliana van de Craats, [http://community.ejc.net/forum/topic/show?id=2345725%3ATopic%3A89026&xgs=1&xg_source=msg_share_topic]<br/><br />
'''Georgia_Tech''': ''<br />
Georgia Tech workshop well received: Lambert students embrace engineering'', ForsythNews.com, [http://www.forsythnews.com/m/section/3/article/14721/. ]<br/><br />
'''Cornell''': ''Using electroactive bacteria, students design toxin sensor'', Cornell Chronicle, [http://www.news.cornell.edu/stories/Oct12/oilSands.html]</div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-labbook-arrow-up-small.pngFile:Ucl2012-labbook-arrow-up-small.png2012-09-28T16:47:58Z<p>PhilippBoeing: uploaded a new version of &quot;File:Ucl2012-labbook-arrow-up-small.png&quot;</p>
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<div></div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-labbook-graph7-2.pngFile:Ucl2012-labbook-graph7-2.png2012-09-28T16:28:07Z<p>PhilippBoeing: uploaded a new version of &quot;File:Ucl2012-labbook-graph7-2.png&quot;</p>
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<div></div>PhilippBoeinghttp://2012.igem.org/File:Ucl2012-labbook-graph6-1.pngFile:Ucl2012-labbook-graph6-1.png2012-09-28T16:26:06Z<p>PhilippBoeing: uploaded a new version of &quot;File:Ucl2012-labbook-graph6-1.png&quot;</p>
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