http://2012.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=250&target=H.jepsenburger&year=&month=
2012.igem.org - User contributions [en]
2024-03-29T13:55:22Z
From 2012.igem.org
MediaWiki 1.16.0
http://2012.igem.org/World_Championship_Jamboree/Schedule/Practice_sessions
World Championship Jamboree/Schedule/Practice sessions
2012-10-31T20:15:11Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<style type="text/css"><br />
h2 {<br />
border-bottom-width: 0px;<br />
border-bottom-style: none;<br />
margin-bottom: 0px;<br />
}<br />
<br />
h2.date {<br />
display: none;<br />
}<br />
<br />
table.calendar {<br />
border-collapse: collapse;<br />
font-size: 12px;<br />
width: 900px;<br />
border-bottom: 2px solid #AAAAAA;<br />
}<br />
<br />
<br />
table.calendar thead th {<br />
border-collapse: separate;<br />
text-align: center;<br />
border-bottom: 2px solid #AAAAAA;<br />
color: #494949;<br />
font-weight: bold;<br />
background-color: #e6ecf6<br />
}<br />
<br />
table.calendar tbody {<br />
margin: 2px;<br />
}<br />
<br />
table.calendar tbody th {<br />
color: #494949;<br />
font-weight: normal;<br />
font-size: 11px;<br />
border-bottom: 1px solid #DDDDDD;<br />
text-align: left;<br />
}<br />
<br />
table.calendar tr:hover { <br />
background-color: #e6ecf6; <br />
}<br />
<br />
table.calendar td {<br />
text-align: center;<br />
border: 1px solid #DDDDDD;<br />
}<br />
</style><br />
</head><br />
</html><br />
<br />
__NOTOC__<br />
{{WCJMainPageCSS}}<br />
<html><br />
<body><br />
<br />
<table align="center" style="width: 950px; margin-top:-10px;"><br />
<br><br />
<td colspan="3" valign="top"><br />
<div class="main_item"> <!-- World Jamboree Schedule Goes Here--> <br />
<div class="title">Presentation Practice: Friday November 2nd 2012</div><br />
<br />
Use this sign-up sheet to sign up for a slot on Friday night (November 2nd) to practice your talk. Note that there will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. Be sure to bring necessary computer equipment with you, such as chargers and adapters, as these will not be provided.<br><br> <br />
<br />
There are a limited number of time slots available on a first-come first-serve basis so please only choose one slot. We cannot match the room that you will ultimately give your presentation in with the practice room. This should, however, give you a chance to practice your talk in a new environment. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time.<br><br><br />
<br />
Also, pre-registration will be available on Friday November 2nd<!-- beginning at <strong>1pm at Compton Lounge</strong>. Conference services will be on-site to pass out team registration boxes (see the <a href="https://2012.igem.org/World_Championship_Jamboree/Handbook">Jamboree Handbook</a>) -->. <br><br><br />
<br />
<strong>Note</strong>: Use the wiki edit button to add your team to the schedule (the markup is located at the bottom of the page). Additional rooms may be added in the coming weeks.<br />
<br />
</div><br />
</td><br />
</tr><br />
</table><br />
</body><br />
</html><br />
<br />
<html> <!--Practice Session Sign-Up Sheet - ADD YOUR TEAM NAME HERE!--><br />
<table class="calendar" align="center"><h2 class="date"><a name="Friday Practice">Friday, November 2</a></h2><br />
<thead><br />
<tr><br />
<th style="width:100px;">Time</th><br />
<th>RM 32-123 </th><br />
<th>RM 34-101 </th><br />
<th>RM 10-250 </th><br />
<th>RM 32-155 </th><br />
<th>RM 32-141 </th><br />
<th>RM 56-154 </th><br />
<th>RM 56-114 </th><br />
</tr><br />
</thead><br />
<tbody><br />
<tr class="even"><br />
<th>5:30 - 6:00PM</th><br />
<td>Tokyo_Tech</td><br />
<td>CBNU-Korea</td><br />
<td>SJTU-BioX-Shanghai</td><br />
<td>Kyoto</td><br />
<td>TU_Munich</td><br />
<td>Wageningen_UR</td><br />
<td>ZJU-China</td><br />
</tr><br />
<tr class="even"><br />
<th>6:00 - 6:30PM</th><br />
<td>Evry</td><br />
<td>UC Chile</td><br />
<td>XMU-China</td><br />
<td>UT-Tokyo-Software</td><br />
<td>Macquarie-Australia</td><br />
<td>HKUST-Hong_Kong</td><br />
<td>Edinburgh</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30 - 7:00PM</th><br />
<td>Paris_Bettencourt</td><br />
<td>University College London</td><br />
<td>INSA de Lyon</td><br />
<td>USTC-Software</td><br />
<td>UNITN-TRENTO</td><br />
<td>Hong_Kong-CUHK</td><br />
<td>TU Munich</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00 - 7:30PM</th><br />
<td>Trieste</td><br />
<td>ASU collegiate</td><br />
<td>Potsdam_Bioware</td><br />
<td>Freiburg</td><br />
<td>Bielefeld-Germany</td><br />
<td>Wisconsin-Madison</td><br />
<td>Tsinghua-A</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30 - 8:00PM</th><br />
<td>Buenos Aires</td><br />
<td>ASU iGEM-E</td><br />
<td>Colombia</td><br />
<td>MIT</td><br />
<td>OUC-China</td><br />
<td>E6</td><br />
<td>E7</td><br />
</tr><br />
<tr class="odd"><br />
<th>8:00 - 8:30PM</th><br />
<td>ETH Zurich</td><br />
<td>Groningen</td><br />
<td>UNAM Genomics Mexico</td><br />
<td>Tianjin</td><br />
<td>KAIST_Korea</td><br />
<td>Nevada</td><br />
<td>F7</td><br />
</tr><br />
<tr class="even"><br />
<th>8:30 - 9:00PM</th><br />
<td>TU Delft</td><br />
<td>NCTU_Formosa</td><br />
<td>Peking</td><br />
<td>SYSU-Software</td><br />
<td>Penn_State</td><br />
<td>UIUC-Illinois</td><br />
<td>G7</td><br />
</tr><br />
<tr class="odd"><br />
<th>9:00 - 9:30PM</th><br />
<td>Northwestern</td><br />
<td>LMU-Munich</td><br />
<td>WHU-China</td><br />
<td>Johns_Hopkins_Software</td><br />
<td>Cambridge</td><br />
<td>H6</td><br />
<td>H7</td><br />
</tr><br />
<tr class="even"><br />
<th>9:30 - 10:00PM</th><br />
<td>Queens_Canada</td><br />
<td>CINVESTAV-IPN-UNAM MX</td><br />
<td>Calgary</td><br />
<td>Penn</td><br />
<td>Berkeley</td><br />
<td>NYMU-Taipei</td><br />
<td>Utah_State</td><br />
</tr><br />
</tbody><br />
</table><br />
<br><br><center><br />
<iframe width="425" height="350" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="http://www.google.com/maps/ms?vpsrc=6&amp;ctz=240&amp;ie=UTF8&amp;msa=0&amp;t=m&amp;msid=208072632249057954752.0004b024a38f4f8325221&amp;source=embed&amp;ll=42.359617,-71.091839&amp;spn=0.000808,0.002135&amp;output=embed"></iframe><br /><small>View <a href="http://www.google.com/maps/ms?vpsrc=6&amp;ctz=240&amp;ie=UTF8&amp;msa=0&amp;t=m&amp;msid=208072632249057954752.0004b024a38f4f8325221&amp;source=embed&amp;ll=42.359617,-71.091839&amp;spn=0.000808,0.002135" style="color:#0000FF;text-align:left">My Saved Places</a> in a larger map</small></center><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Modeling
Team:Penn State/Modeling
2012-10-27T03:59:12Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Main Results</h3><br />
<p>Included here are the pooled results of each project. All results are measurements of fluorescence performed by a TECANM1000 instrument. For detailed discussion, please see the individual <a href="href="https://2012.igem.org/Team:Penn_State/Project>project pages</a>. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>BiDirectional Promoter Project Data</h2><br />
<p>Here we can see some of the data collected for the Bidirectional Promoter Project. The data shows the fluorescence of GFP and RFP from different promoters collected by the TCAN.<br />
</p><br />
<p>GFP Data for all of the samples tested and the average of those samples for a given construct. The promoters tested so far are <a href="http://partsregistry.org/Part:BBa_J23102">BBa_J23102</a>, and three custom palindromic promoters. </p><br />
<img src="https://static.igem.org/mediawiki/2012/f/fc/BiDirresults.png"></br><br />
<h2>Multiple Start Codon Project Data</h2><br />
<p>Here is some of the preliminary data for the MSC Project. The graph depicts the fluorescence measured by expression from each individual start codon.</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/24/MSC_results.png"></br><br />
<h2>Codon Optimization</h2><br />
<p>Here is some of the preliminary data for the Codon Optimization Project. The graph depicts the fluorescence measured in each threonine codon 6-repeat sequence.</p><br />
<img src="https://static.igem.org/mediawiki/2012/c/cc/Threonine6_results.png"><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Modeling
Team:Penn State/Modeling
2012-10-27T03:58:35Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Main Results</h3><br />
<p>Included here are the pooled results of each project. All results are measurements of fluorescence performed by a TECANM1000 instrument. For detailed discussion, please see the individual <a href="href="https://2012.igem.org/Team:Penn_State/Project>project pages</a>. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>BiDirectional Promoter Project Data</h2><br />
<p>Here we can see some of the data collected for the Bidirectional Promoter Project. The data shows the fluorescence of GFP and RFP from different promoters collected by the TCAN.<br />
</p><br />
<p>GFP Data for all of the samples tested and the average of those samples for a given construct. The promoters tested so far are <a href="http://partsregistry.org/Part:BBa_J23102">BBa_J23102</a>, and three custom palindromic promoters. </p><br />
<img src="https://static.igem.org/mediawiki/2012/f/fc/BiDirresults.png"></br><br />
<h2>Multiple Start Codon Project Data</h2><br />
<p>Here is some of the preliminary data for the MSC Project. The graph depicts the fluorescence measured by expression from each individual start codon.</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/24/MSC_results.png"></br><br />
<h3>Codon Optimization</h3><br />
<p>Here is some of the preliminary data for the Codon Optimization Project. The graph depicts the fluorescence measured in each threonine codon 6-repeat sequence.</p><br />
<img src="https://static.igem.org/mediawiki/2012/c/cc/Threonine6_results.png"><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results
Team:Penn State/Bidirectional Promoters Results
2012-10-27T03:53:13Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color: #666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
ul {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0;<br />
margin: 0;<br />
width: 570px;<br />
color: #333;<br />
list-style-position: inside;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Bidirectional Promoters</h3></br><br />
<p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce <br />
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote <br />
and the degree of expression has not been quantified. This project will test the directionality of <br />
several BioBrick promoters to answer these questions. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Bidirectional Promoters</h2><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Palindromic Promoter Circuits</h3><br />
<p><img src="https://static.igem.org/mediawiki/2012/f/fc/BiDirresults.png"></p><br />
<p>The graph above represents the relative fluorescence measured in a TECANM1000 instrument of several constructs containing various promoter inserts. The reporters indicate the directionality of each promoter sequence measured in the construct. The single biobrick ligated into the construct shows almost complete preference for the forward direction, while three palindromic sequences designed and ligated in the construct demonstrate varying amounts of bidirectionality within the DH10B E.coli cell. Bidirectional promoters are possible within the cell, and those designing promoters should be beware of palindromic sequences in their design and their potential effects on the initiation of protein within the cell. </p><br />
<br />
<h3>Further Application</h3><br />
<p>Further characterization of both biobrick promoters and palindromic sequences will yield more information of the prevalence of bidirectionality in promoters. To view the sequences we plan to test in this construct, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">parts</a> page.</p><br />
<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Results
Team:Penn State/MSC Results
2012-10-27T03:41:56Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {clear:both; margin-top: 15px;}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
.activemaintab {background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#333; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activemaintab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(../images/indexNav1.jpg);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {color: #666}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3></br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/MultipleStartCodons">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>TECAN Data </h3><br />
<p>The initial circuit for this project has recently been completed. The relative fluorescence of both RFP and sfGFP reporters was tested in a TECANM1000.</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/24/MSC_results.png"><br />
</p><br />
<p> The graph above shows the relative fluorescence of two reporters corresponding to two start codons in the circuit. The reporters indicate the ribosome's preference for one start codon over the other in protein translation. Super-folder GFP (sfGFP) is preferred in this construct, as shown by the high amount of relative fluorescence. Though the amount of green fluorescence significantly outweighs the red fluorescence, there is still a measurable amount of RFP expression in the cell. The presence of both reporters indicates that multiple start codons may be possible in DH10B E.coli, since the ribosome recognizes both start codons and initiates the translation of both proteins.</p></br><br />
<p><h3>Further Application</h3></p><br />
<p>This construct tests only a 7-nucleotide separation between start codons. Since these preliminary results show that the recognition of multiple start codons is possible within the cell, further research will continue to investigate the effect of ribosome binding site translation initiation rate, as well as length between codons, on codon slippage. To view the full list of intended sequences which will be tested in this genetic circuit, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">parts</a> page. </P><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Results
Team:Penn State/MSC Results
2012-10-27T03:41:12Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {clear:both; margin-top: 15px;}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
.activemaintab {background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#333; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activemaintab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(../images/indexNav1.jpg);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {color: #666}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3></br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/MultipleStartCodons">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>TECAN Data </h3><br />
<p>The initial circuit for this project has recently been completed. The relative fluorescence of both RFP and sfGFP reporters was tested in a TECANM1000.</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/24/MSC_results.png"><br />
</p><br />
<p> The graph above shows the relative fluorescence of two reporters corresponding to two start codons in the circuit. The reporters indicate the ribosome's preference for one start codon over the other in protein translation. Super-folder GFP (sfGFP) is preferred in this construct, as shown by the high amount of relative fluorescence. Though the amount of green fluorescence significantly outweighs the red fluorescence, there is still a measurable amount of RFP expression in the cell. The presence of both reporters indicates that multiple start codons may be possible in DH10B E.coli, since the ribosome recognizes both start codons and initiates the translation of both proteins.</p></br><br />
<p>Further Application</p><br />
<p>This construct tests only a 7-nucleotide separation between start codons. Since these preliminary results show that the recognition of multiple start codons is possible within the cell, further research will continue to investigate the effect of ribosome binding site translation initiation rate, as well as length between codons, on codon slippage. To view the full list of intended sequences which will be tested in this genetic circuit, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">parts</a> page. </P><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:MSC_results.png
File:MSC results.png
2012-10-27T03:39:12Z
<p>H.jepsenburger: uploaded a new version of &quot;File:MSC results.png&quot;</p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/File:MSC_results.png
File:MSC results.png
2012-10-27T03:38:02Z
<p>H.jepsenburger: uploaded a new version of &quot;File:MSC results.png&quot;</p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Results
Team:Penn State/MSC Results
2012-10-27T03:37:02Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {clear:both; margin-top: 15px;}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
.activemaintab {background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#333; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activemaintab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(../images/indexNav1.jpg);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {color: #666}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3></br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/MultipleStartCodons">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>TECAN Data </h3><br />
<p>The initial circuit for this project has recently been completed. The relative fluorescence of both RFP and sfGFP reporters was tested in a TECANM1000.</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/24/MSC_results.png"><br />
</p><br />
<p> The graph above shows the relative fluorescence of two reporters corresponding to two start codons in the circuit. The reporters indicate the ribosome's preference for one start codon over the other in protein translation. Super-folder GFP (sfGFP) is preferred in this construct, as shown by the high amount of relative fluorescence. Though the amount of green fluorescence significantly outweighs the red fluorescence, there is still a measurable amount of RFP expression in the cell. The presence of both reporters indicates that multiple start codons may be possible in DH10B E.coli, since the ribosome recognizes both start codons and initiates the translation of both proteins.</p></br><br />
<p>Further Application</p><br />
This construct tests only a 7-nucleotide separation between start codons. Since these preliminary results show that the recognition of multiple start codons is possible within the cell, further research will continue to investigate the effect of ribosome binding site translation initiation rate, as well as length between codons, on codon slippage. To view the full list of intended sequences which will be tested in this genetic circuit, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">parts</a> page. <br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Codon_Optimization_Results
Team:Penn State/Codon Optimization Results
2012-10-27T03:23:23Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666;}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Codon Optimization</h3></br><br />
<p>The genetic code is a degenerate one; there are more 3-part combinations of nucleotides than there are amino acids. The topic of codon optimization-that is, the cell's preference for one codon sequence over another in translation-has been heavily researched in an effort to determine the optimal genetic sequences for an organism. This project looks at the effects of repeated amino acid sequences of varying lengths and codons and their effect on the cell. </p> <br />
</div><br />
<br />
<div id="main"><br />
<h2>Codon Optimization</h2><br />
<iframe width="420" height="315" src="http://www.youtube.com/embed/uspXR2UzP0U" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Data Collection</h3><br />
<p>A 6-repeated sequence of a single threonine codon was successfully ligated into the initial construct. The resulting fluorescence was measured using a TECAN machine. The results are displayed graphically below.</p><br />
<P><img src="https://static.igem.org/mediawiki/2012/c/cc/Threonine6_results.png"></p><br />
<br />
<P>The figure above demonstrates the fluorescence of both mCherry and GFP when the ribosome translates four 6-repeat sequences of a single codon of threonine. mCherry levels fluctuate while GFP fluorescence remains the same. Further characterization with repeating sequences will provide more quantitative data, but preliminary testing suggests that with constant levels of GFP, the cell is able to send tRNA strands complementary to each threonine codon without disturbing GFP production. Thus, the codon sequence with the highest mCherry expression indicates the most preferred threonine codon.</p> <br />
<br />
<h3>Additional Testing</h3><br />
<p>Several more repeated sequences of identical and non-identical single amino acid codons are intended for testing in this construct. To view the <a href="https://2012.igem.org/Team:Penn_State/Parts">completed and prospective sequences</a>, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a> page<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Codon_Optimization_Design
Team:Penn State/Codon Optimization Design
2012-10-27T03:21:12Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666;}<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Codon Optimization</h3></br><br />
<p>The genetic code is a degenerate one; there are more 3-part combinations of nucleotides than there are amino acids. The topic of codon optimization-that is, the cell's preference for one codon sequence over another in translation-has been heavily researched in an effort to determine the optimal genetic sequences for an organism. This project looks at the effects of repeated amino acid sequences of varying lengths and codons and their effect on the cell. </p> <br />
</div><br />
<br />
<div id="main"><br />
<h2>Codon Optimization</h2><br />
<iframe width="420" height="315" src="http://www.youtube.com/embed/uspXR2UzP0U" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Circuit Model</h3><p><img src="https://static.igem.org/mediawiki/2012/7/76/CodOpt_design.png"></p><br />
<h3>Initial Design</h3><br />
<p>To test the effect of repeated codon sequences on cell protein expression, we altered a plasmid available in the Richard lab to contain a promoter, ribosome binding site, and an AHL leader sequence, followed by a non-coding repeated sequence placeholder flanked by restriction enzyme sites. A mCherry and GFP reporter allowed us to test each repeated codon sequence.</p> <br />
<br />
<h3>Sources</h3><br />
<p>The initial construct was partially assembled by a Ph.D student formerly working in the Richard Lab at Penn State named Mike Speer. This project was designed by Mr. Speer, but never completed. The Penn State iGEM team worked closely with Mr. Speer to successfully complete the initial construct.</p><br />
<br />
<h3>Assembly</h3><br />
<p>This part was assembled using Amplified Insert Assembly, a technique developed in the Richard Lab by the 2011 Penn State iGEM team. AIA utilizes the complementary sticky ends left by certain restriction enzymes to ligate inserts and vectors with benign scars.<br />
</p><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/MSC_Overview">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Overview">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Overview">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:CodOpt_design.png
File:CodOpt design.png
2012-10-27T03:20:24Z
<p>H.jepsenburger: uploaded a new version of &quot;File:CodOpt design.png&quot;</p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design
Team:Penn State/Bidirectional Promoters Design
2012-10-27T03:19:55Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color: #666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
ul {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0;<br />
margin: 0;<br />
width: 570px;<br />
color: #333;<br />
list-style-position: inside;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Bidirectional Promoters</h3><br />
<p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce <br />
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote <br />
and the degree of expression has not been quantified. This project will test the directionality of <br />
several BioBrick promoters to answer these questions. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Bidirectional Promoters</h2><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Circuit Model</h3><p><img src="https://static.igem.org/mediawiki/2012/6/65/BiDir_design.png"></p><br />
<h3>Initial Design</h3><br />
<p>This construct tests the directionality of selected promoters. A small non-coding sequence lies between two restriction enzyme sites; this is the area where each promoter will be inserted. Upstream from the promoter sequence lies a RFP reporter. Downstream codes for a GFP reporter. Thus, as promoters are ligated into the promoter placeholder, the cell fluoresces red, green, or both, indicating the promoter directionality.</p><br />
<br />
<h3>Sources</h3><br />
<p>The initial construct for this project utilized the reporters of non-BioBrick parts available in a neighboring campus lab. The Salis Lab generously allowed us to use samples of the pBAC plasmid and mFTV3 plasmid to assemble the construct.</p><br />
<br />
<h3>Assembly</h3><br />
<p>The pBAC vector was PCR amplified with 40 basepair overlaps complementary to the ends of the reporter inserts. A RFP reporter and GFP reporter were simultaneously PCR amplified from the mFTV3 plasmid, both containing a 40 basepair overlap sequence complementary to the ends of the adjacent piece. <br />
<p>These parts were finally assembled using the CBAR, or Gibson Assembly, reaction. Gibson Assembly utilizes three enzymes.</p><br />
<ul><br />
<li><p>An exonuclease, which chews back the overlapping sequences of each piece from the 5' to 3' ends</p></li><br />
<li><p>A polymerase, which fills in the gaps left in the DNA from the exonuclease</p></li><br />
<li><p>A ligase, which heals the nicks in the annealed DNA strand</p></li><br />
</ul><br />
<br />
<p>Annealing the pieces together provided a construct devoid of an actual promoter sequence. By digesting the construct with restriction enzymes flanking the promoter placeholder and inserting promoters isolated from BioBrick parts, the construct fulfilled its purpose.</p> <br />
<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design
Team:Penn State/Bidirectional Promoters Design
2012-10-27T03:19:21Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color: #666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
ul {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0;<br />
margin: 0;<br />
width: 570px;<br />
color: #333;<br />
list-style-position: inside;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Projects">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Bidirectional Promoters</h3><br />
<p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce <br />
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote <br />
and the degree of expression has not been quantified. This project will test the directionality of <br />
several BioBrick promoters to answer these questions. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Bidirectional Promoters</h2><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Circuit Model</h3><p><img src="https://static.igem.org/mediawiki/2012/6/65/BiDir_design.png"></p><br />
<h3>Initial Design</h3><br />
<p>This construct tests the directionality of selected promoters. A small non-coding sequence lies between two restriction enzyme sites; this is the area where each promoter will be inserted. Upstream from the promoter sequence lies a RFP reporter. Downstream codes for a GFP reporter. Thus, as promoters are ligated into the promoter placeholder, the cell fluoresces red, green, or both, indicating the promoter directionality.</p><br />
<br />
<h3>Sources</h3><br />
<p>The initial construct for this project utilized the reporters of non-BioBrick parts available in a neighboring campus lab. The Salis Lab generously allowed us to use samples of the pBAC plasmid and mFTV3 plasmid to assemble the construct.</p><br />
<br />
<h3>Assembly</h3><br />
<p>The pBAC vector was PCR amplified with 40 basepair overlaps complementary to the ends of the reporter inserts. A RFP reporter and GFP reporter were simultaneously PCR amplified from the mFTV3 plasmid, both containing a 40 basepair overlap sequence complementary to the ends of the adjacent piece. <br />
<p>These parts were finally assembled using the CBAR, or Gibson Assembly, reaction. Gibson Assembly utilizes three enzymes.</p><br />
<ul><br />
<li><p>An exonuclease, which chews back the overlapping sequences of each piece from the 5' to 3' ends</p></li><br />
<li><p>A polymerase, which fills in the gaps left in the DNA from the exonuclease</p></li><br />
<li><p>A ligase, which heals the nicks in the annealed DNA strand</p></li><br />
</ul><br />
<br />
<p>Annealing the pieces together provided a construct devoid of an actual promoter sequence. By digesting the construct with restriction enzymes flanking the promoter placeholder and inserting promoters isolated from BioBrick parts, the construct fulfilled its purpose.</p> <br />
<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:BiDir_design.png
File:BiDir design.png
2012-10-27T03:18:55Z
<p>H.jepsenburger: </p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Design
Team:Penn State/MSC Design
2012-10-27T03:17:37Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
<br />
body {<br />
background:#e0e0e0;<br />
color:#303030;<br />
font:76% tahoma, verdana, sans-serif;<br />
margin:0;<br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {<br />
border:none;<br />
color:#505050;<br />
}<br />
#logo h1 a:hover {<br />
border:none;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
#logo p {<br />
font-size:1.3em;<br />
color:#505050;<br />
margin:0 0 20px 0;<br />
}<br />
<br />
<br />
/* Tags */<br />
<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {<br />
border-bottom:1px solid #505050;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
a img {<br />
border:0;<br />
}<br />
h1 {<br />
font-size:2.2em;<br />
font-weight:400;<br />
margin:0 0 5px;<br />
padding:0;<br />
}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {<br />
font-size:1.4em;<br />
font-weight:400;<br />
margin:0 0 10px;<br />
}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {<br />
margin:0 0 15px 15px;<br />
padding:0;<br />
}<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
<br />
#navitabs {<br />
clear:both;<br />
}<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
border-left:1px solid #fff;<br />
font-size:1.1em;<br />
margin:0 0 4px;<br />
padding:4px 8px;<br />
text-decoration:none;<br />
}<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#fff;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activenavitab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Main content */<br />
<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
<br />
#sidebar {<br />
float:right;<br />
width:150px;<br />
}<br />
#sidebar p {<br />
font-size:0.9em;<br />
line-height:1.3em;<br />
margin:0 0 15px;<br />
}<br />
#sidebar ul {<br />
margin:0 0 15px 0;<br />
padding:0;<br />
}<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
ul.sidelink li {<br />
list-style:none;<br />
margin:0 0 3px;<br />
padding:0;<br />
}<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
display:block;<br />
margin:5px 10px 5px 0;<br />
padding:3px 4px 3px 8px;<br />
text-align:left; width:140px;<br />
}<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
<br />
#footer {<br />
background:#fff;<br />
border-top:1px solid #d8d8d8;<br />
clear:both;<br />
margin:0;<br />
padding:0;<br />
}<br />
#footer a {<br />
color:gray;<br />
}<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
#footer p {<br />
color:gray;<br />
font-size:1.1em;<br />
line-height:1.3em;<br />
margin:15px 0 0;<br />
padding:0;<br />
}<br />
<br />
<br />
/* Additional classes */<br />
<br />
.photo {<br />
background-color:#f0f0f0;<br />
border:1px solid #d8d8d8;<br />
margin:0 0 15px;<br />
padding:2px;<br />
}<br />
.right {<br />
margin:-8px 0 8px 0;<br />
text-align:right;<br />
}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {<br />
display:none;<br />
color: #000;<br />
}<br />
<br />
/*Tabbed Panels*/<br />
<br />
#maintabs {<br />
clear:both;<br />
margin-top: 10px;<br />
}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
margin-top: 15px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#333;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activemaintab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
#prezi {<br />
width: 300px;<br />
height: 200px;<br />
background-color: #036;<br />
clear: both;<br />
margin-bottom: 10px;<br />
padding-bottom: 15px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {<br />
color: #oc9<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3><br />
</br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<br />
<div id="maintabs"><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Circuit Model</h3><p><img src="https://static.igem.org/mediawiki/2012/7/76/CodOpt_design.png"></P><br />
<h3>Circuit Design</h3><br />
<p>To begin construction of this plasmid, the DNA sequence was generated and edited in ApE, a DNA editing software available through the University of Utah, from several existing plasmid sequences available through other labs on the Penn State campus. The construct was rationally designed to include two start codons within 7 nucleotides of each other, and thus in different open reading frames. <p> Each codon, labeled RFP-coding ATG and sfGFP-coding ATG for simplicity, codes for a different reporter in the respective reading frame of each start codon. The first start codon, RFP-coding ATG, if recognized by the ribosome, will translate the reporter RFP. The second start codon, sfGFP-codon ATG, will translate the reporter sfGFP if recognized by the ribosome. Super-folder GFP (sfGFP) is used to allow for the nonsense codons of the out-of-frame RFP sequence preceding the sfGFP reporter which would inevitably be translated prior to the green reporter sequence should the ribosome recognize the sfGFP-coding ATG. The super-folder protein will fold regardless of this nonsense sequence, facilitating the measurement of codon slippage through the measurement of fluorescence</p><br />
<p>To prevent premature termination of the translation of either reporter, all stop codons in the respective reading frames of each codon were eliminated through amino acid sequence substitution.</p><br />
<h3>Circuit Construction</h3><br />
<p>Initially, 4 gBlocks, available through IDT as oligos with overlapping 40bp sequences complimentary to the adjacent oligo, were used to perform a Gibson Assembly reaction with a digested dRBS1 vector. After several failed attempts, a second approach was implemented. Oligos for the missing circuit sequence were designed and annealed, according to their complimentary 20bp overlaps, and inserted into the nearly-complete vector using Gibson Assembly. This attempt was successful, and the initial construct was verified with sequencing.</P> <br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Design
Team:Penn State/MSC Design
2012-10-27T03:17:05Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
<br />
body {<br />
background:#e0e0e0;<br />
color:#303030;<br />
font:76% tahoma, verdana, sans-serif;<br />
margin:0;<br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {<br />
border:none;<br />
color:#505050;<br />
}<br />
#logo h1 a:hover {<br />
border:none;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
#logo p {<br />
font-size:1.3em;<br />
color:#505050;<br />
margin:0 0 20px 0;<br />
}<br />
<br />
<br />
/* Tags */<br />
<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {<br />
border-bottom:1px solid #505050;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
a img {<br />
border:0;<br />
}<br />
h1 {<br />
font-size:2.2em;<br />
font-weight:400;<br />
margin:0 0 5px;<br />
padding:0;<br />
}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {<br />
font-size:1.4em;<br />
font-weight:400;<br />
margin:0 0 10px;<br />
}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {<br />
margin:0 0 15px 15px;<br />
padding:0;<br />
}<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
<br />
#navitabs {<br />
clear:both;<br />
}<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
border-left:1px solid #fff;<br />
font-size:1.1em;<br />
margin:0 0 4px;<br />
padding:4px 8px;<br />
text-decoration:none;<br />
}<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#fff;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activenavitab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Main content */<br />
<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
<br />
#sidebar {<br />
float:right;<br />
width:150px;<br />
}<br />
#sidebar p {<br />
font-size:0.9em;<br />
line-height:1.3em;<br />
margin:0 0 15px;<br />
}<br />
#sidebar ul {<br />
margin:0 0 15px 0;<br />
padding:0;<br />
}<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
ul.sidelink li {<br />
list-style:none;<br />
margin:0 0 3px;<br />
padding:0;<br />
}<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
display:block;<br />
margin:5px 10px 5px 0;<br />
padding:3px 4px 3px 8px;<br />
text-align:left; width:140px;<br />
}<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
<br />
#footer {<br />
background:#fff;<br />
border-top:1px solid #d8d8d8;<br />
clear:both;<br />
margin:0;<br />
padding:0;<br />
}<br />
#footer a {<br />
color:gray;<br />
}<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
#footer p {<br />
color:gray;<br />
font-size:1.1em;<br />
line-height:1.3em;<br />
margin:15px 0 0;<br />
padding:0;<br />
}<br />
<br />
<br />
/* Additional classes */<br />
<br />
.photo {<br />
background-color:#f0f0f0;<br />
border:1px solid #d8d8d8;<br />
margin:0 0 15px;<br />
padding:2px;<br />
}<br />
.right {<br />
margin:-8px 0 8px 0;<br />
text-align:right;<br />
}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {<br />
display:none;<br />
color: #000;<br />
}<br />
<br />
/*Tabbed Panels*/<br />
<br />
#maintabs {<br />
clear:both;<br />
margin-top: 10px;<br />
}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
margin-top: 15px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#333;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activemaintab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
#prezi {<br />
width: 300px;<br />
height: 200px;<br />
background-color: #036;<br />
clear: both;<br />
margin-bottom: 10px;<br />
padding-bottom: 15px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {<br />
color: #oc9<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3><br />
</br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<br />
<div id="maintabs"><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>circuit Model/h3><p><img src="https://static.igem.org/mediawiki/2012/7/76/CodOpt_design.png"></P><br />
<h3>Circuit Design</h3><br />
<p>To begin construction of this plasmid, the DNA sequence was generated and edited in ApE, a DNA editing software available through the University of Utah, from several existing plasmid sequences available through other labs on the Penn State campus. The construct was rationally designed to include two start codons within 7 nucleotides of each other, and thus in different open reading frames. <p> Each codon, labeled RFP-coding ATG and sfGFP-coding ATG for simplicity, codes for a different reporter in the respective reading frame of each start codon. The first start codon, RFP-coding ATG, if recognized by the ribosome, will translate the reporter RFP. The second start codon, sfGFP-codon ATG, will translate the reporter sfGFP if recognized by the ribosome. Super-folder GFP (sfGFP) is used to allow for the nonsense codons of the out-of-frame RFP sequence preceding the sfGFP reporter which would inevitably be translated prior to the green reporter sequence should the ribosome recognize the sfGFP-coding ATG. The super-folder protein will fold regardless of this nonsense sequence, facilitating the measurement of codon slippage through the measurement of fluorescence</p><br />
<p>To prevent premature termination of the translation of either reporter, all stop codons in the respective reading frames of each codon were eliminated through amino acid sequence substitution.</p><br />
<h3>Circuit Construction</h3><br />
<p>Initially, 4 gBlocks, available through IDT as oligos with overlapping 40bp sequences complimentary to the adjacent oligo, were used to perform a Gibson Assembly reaction with a digested dRBS1 vector. After several failed attempts, a second approach was implemented. Oligos for the missing circuit sequence were designed and annealed, according to their complimentary 20bp overlaps, and inserted into the nearly-complete vector using Gibson Assembly. This attempt was successful, and the initial construct was verified with sequencing.</P> <br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:CodOpt_design.png
File:CodOpt design.png
2012-10-27T03:16:00Z
<p>H.jepsenburger: </p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Codon_Optimization_Results
Team:Penn State/Codon Optimization Results
2012-10-27T03:07:56Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666;}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Codon Optimization</h3></br><br />
<p>The genetic code is a degenerate one; there are more 3-part combinations of nucleotides than there are amino acids. The topic of codon optimization-that is, the cell's preference for one codon sequence over another in translation-has been heavily researched in an effort to determine the optimal genetic sequences for an organism. This project looks at the effects of repeated amino acid sequences of varying lengths and codons and their effect on the cell. </p> <br />
</div><br />
<br />
<div id="main"><br />
<h2>Codon Optimization</h2><br />
<iframe width="420" height="315" src="http://www.youtube.com/embed/uspXR2UzP0U" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Data Collection</h3><br />
<p>A 6-repeated sequence of a single threonine codon was successfully ligated into the initial construct. The resulting fluorescence was measured using a TECAN machine. The results are displayed graphically below.</p><br />
<P><img src="https://static.igem.org/mediawiki/2012/c/cc/Threonine6_results.png"></p><br />
<br />
<P>The figure above demonstrates the fluorescence of both mCherry and GFP when the ribosome translates four 6-repeat sequences of a single codon of threonine. mCherry levels fluctuate while GFP fluorescence remains the same. Further characterization with repeating sequences will provide more quantitative data, but preliminary testing suggests that with constant levels of GFP, the cell is able to send tRNA strands complementary to each threonine codon without disturbing GFP production. Thus, the codon sequence with the highest mCherry expression indicates the most preferred threonine codon.</p> <br />
<br />
<h3>Additional Testing</h3><br />
<p>Several more repeated sequences of identical and non-identical single amino acid codons are intended for testing in this construct. To view the <a href="https://2012.igem.org/Team:Penn_State/Parts">completed and prospective sequences</a>, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a> page<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/MSC_Overview">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Codon_Optimization_Design
Team:Penn State/Codon Optimization Design
2012-10-27T03:07:32Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666;}<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Codon Optimization</h3></br><br />
<p>The genetic code is a degenerate one; there are more 3-part combinations of nucleotides than there are amino acids. The topic of codon optimization-that is, the cell's preference for one codon sequence over another in translation-has been heavily researched in an effort to determine the optimal genetic sequences for an organism. This project looks at the effects of repeated amino acid sequences of varying lengths and codons and their effect on the cell. </p> <br />
</div><br />
<br />
<div id="main"><br />
<h2>Codon Optimization</h2><br />
<iframe width="420" height="315" src="http://www.youtube.com/embed/uspXR2UzP0U" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Initial Design</h3><br />
<p>To test the effect of repeated codon sequences on cell protein expression, we altered a plasmid available in the Richard lab to contain a promoter, ribosome binding site, and an AHL leader sequence, followed by a non-coding repeated sequence placeholder flanked by restriction enzyme sites. A mCherry and GFP reporter allowed us to test each repeated codon sequence.</p> <br />
<br />
<h3>Sources</h3><br />
<p>The initial construct was partially assembled by a Ph.D student formerly working in the Richard Lab at Penn State named Mike Speer. This project was designed by Mr. Speer, but never completed. The Penn State iGEM team worked closely with Mr. Speer to successfully complete the initial construct.</p><br />
<br />
<h3>Assembly</h3><br />
<p>This part was assembled using Amplified Insert Assembly, a technique developed in the Richard Lab by the 2011 Penn State iGEM team. AIA utilizes the complementary sticky ends left by certain restriction enzymes to ligate inserts and vectors with benign scars.<br />
</p><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/MSC_Overview">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Overview">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Overview">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Codon_Optimization
Team:Penn State/Codon Optimization
2012-10-27T03:07:11Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666;}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
padding-top: 10px;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Codon Optimization</h3></br><br />
<p>The genetic code is a degenerate one; there are more 3-part combinations of nucleotides than there are amino acids. The topic of codon optimization-that is, the cell's preference for one codon sequence over another in translation-has been heavily researched in an effort to determine the optimal genetic sequences for an organism. This project looks at the effects of repeated amino acid sequences of varying lengths and codons and their effect on the cell. </p> <br />
</div><br />
<br />
<div id="main"><br />
<h2>Codon Optimization</h2><br />
<iframe width="420" height="315" src="http://www.youtube.com/embed/uspXR2UzP0U?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Background</h3><br />
<p>All of the proteins around us, with few exceptions, are made up of 20 fundamental building blocks of life - amino acids. Different arrangements and combinations of these basic building blocks give us the diversity of proteins that we see. Messenger RiboNucleic Acids (mRNA) is in essence a "photocopy" of DNA that codes for a gene. mRNA carry codons, which are groups of three bases that code for a single amino acid. There are 64 possible combinations of codons (4 x 4 x 4 = 64), but these combinations are degenerate, so there can be more than one codon that codes for the same amino acid. <br />
</p><br />
<br />
<h3>The Problem</h3><br />
<p>We have mentioned how these can be a number of codons that can code for the same Amino Acid, and subsequently, a number of tRNA molecules that can carry a given Amino Acid. In nature, and in many organisms, only a select few of these tRNA and codon combinations are used instead of all sequences that code for the same amino acid. This is called codon bias.<br />
</p><br />
<br />
<h3>The Objective</h3><br />
<p>Our goal for this project is to see which codons are preferred, or biased. We also want to investigate if this bias can change over time, different circumstances, or stresses. <br />
</p><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results
Team:Penn State/Bidirectional Promoters Results
2012-10-27T03:06:46Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color: #666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
ul {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0;<br />
margin: 0;<br />
width: 570px;<br />
color: #333;<br />
list-style-position: inside;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Bidirectional Promoters</h3></br><br />
<p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce <br />
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote <br />
and the degree of expression has not been quantified. This project will test the directionality of <br />
several BioBrick promoters to answer these questions. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Bidirectional Promoters</h2><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Palindromic Promoter Circuits</h3><br />
<p><img src="https://static.igem.org/mediawiki/2012/f/fc/BiDirresults.png"></p><br />
<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design
Team:Penn State/Bidirectional Promoters Design
2012-10-27T03:06:24Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color: #666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
ul {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0;<br />
margin: 0;<br />
width: 570px;<br />
color: #333;<br />
list-style-position: inside;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Projects">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Bidirectional Promoters</h3><br />
<p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce <br />
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote <br />
and the degree of expression has not been quantified. This project will test the directionality of <br />
several BioBrick promoters to answer these questions. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Bidirectional Promoters</h2><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Initial Design</h3><br />
<p>This construct tests the directionality of selected promoters. A small non-coding sequence lies between two restriction enzyme sites; this is the area where each promoter will be inserted. Upstream from the promoter sequence lies a RFP reporter. Downstream codes for a GFP reporter. Thus, as promoters are ligated into the promoter placeholder, the cell fluoresces red, green, or both, indicating the promoter directionality.</p><br />
<br />
<h3>Sources</h3><br />
<p>The initial construct for this project utilized the reporters of non-BioBrick parts available in a neighboring campus lab. The Salis Lab generously allowed us to use samples of the pBAC plasmid and mFTV3 plasmid to assemble the construct.</p><br />
<br />
<h3>Assembly</h3><br />
<p>The pBAC vector was PCR amplified with 40 basepair overlaps complementary to the ends of the reporter inserts. A RFP reporter and GFP reporter were simultaneously PCR amplified from the mFTV3 plasmid, both containing a 40 basepair overlap sequence complementary to the ends of the adjacent piece. <br />
<p>These parts were finally assembled using the CBAR, or Gibson Assembly, reaction. Gibson Assembly utilizes three enzymes.</p><br />
<ul><br />
<li><p>An exonuclease, which chews back the overlapping sequences of each piece from the 5' to 3' ends</p></li><br />
<li><p>A polymerase, which fills in the gaps left in the DNA from the exonuclease</p></li><br />
<li><p>A ligase, which heals the nicks in the annealed DNA strand</p></li><br />
</ul><br />
<br />
<p>Annealing the pieces together provided a construct devoid of an actual promoter sequence. By digesting the construct with restriction enzymes flanking the promoter placeholder and inserting promoters isolated from BioBrick parts, the construct fulfilled its purpose.</p> <br />
<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters
Team:Penn State/Bidirectional Promoters
2012-10-27T03:05:54Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:visited {color: #666}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; <br />
display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both; margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Bidirectional Promoters</h3><br />
<p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce <br />
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote <br />
and the degree of expression has not been quantified. This project will test the directionality of <br />
several BioBrick promoters to answer these questions. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Bidirectional Promoters</h2><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Overview">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Background</h3><br />
<p>Before we can make a protein, we need an mRNA to carry the information about the order of the amino acids. <br />
But before we can make mRNA we need to know where the genes are in the DNA of a cell. Before RNA polymerase can <br />
make a copy it needs to bind to the DNA. This is assisted by a variety of factors, other proteins, that look for<br />
a specific sequence in the DNA. This sequence is called a promoter because it promotes the transcription of the <br />
DNA into RNA by the RNA polymerase. These sequences are generally upstream, or ahead, of a gene's coding sequence. <br />
</p><br />
<h3>The Problem</h3><br />
<p>Not all promoters cause RNA polymerase to transcribe downstream in the expected "forward direction". Some promoters can cause RNA polymerase to go in the opposite direction from what is expected, or go in both directions. This is what we are trying to find out; do different promoters go in different directions, and what is the directional preference of different promoters.<br />
</p><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Results
Team:Penn State/MSC Results
2012-10-27T03:05:28Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {clear:both; margin-top: 15px;}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
.activemaintab {background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#333; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activemaintab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(../images/indexNav1.jpg);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {color: #666}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3></br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/MultipleStartCodons">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>TECAN Data </h3><br />
<p>The initial circuit for this project has recently been completed. The relative fluorescence of both RFP and sfGFP reporters was tested in a TECANM1000.</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/24/MSC_results.png"><br />
</p><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Design
Team:Penn State/MSC Design
2012-10-27T03:05:03Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
<br />
body {<br />
background:#e0e0e0;<br />
color:#303030;<br />
font:76% tahoma, verdana, sans-serif;<br />
margin:0;<br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {<br />
border:none;<br />
color:#505050;<br />
}<br />
#logo h1 a:hover {<br />
border:none;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
#logo p {<br />
font-size:1.3em;<br />
color:#505050;<br />
margin:0 0 20px 0;<br />
}<br />
<br />
<br />
/* Tags */<br />
<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {<br />
border-bottom:1px solid #505050;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
a img {<br />
border:0;<br />
}<br />
h1 {<br />
font-size:2.2em;<br />
font-weight:400;<br />
margin:0 0 5px;<br />
padding:0;<br />
}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {<br />
font-size:1.4em;<br />
font-weight:400;<br />
margin:0 0 10px;<br />
}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {<br />
margin:0 0 15px 15px;<br />
padding:0;<br />
}<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
<br />
#navitabs {<br />
clear:both;<br />
}<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
border-left:1px solid #fff;<br />
font-size:1.1em;<br />
margin:0 0 4px;<br />
padding:4px 8px;<br />
text-decoration:none;<br />
}<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#fff;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activenavitab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Main content */<br />
<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
<br />
#sidebar {<br />
float:right;<br />
width:150px;<br />
}<br />
#sidebar p {<br />
font-size:0.9em;<br />
line-height:1.3em;<br />
margin:0 0 15px;<br />
}<br />
#sidebar ul {<br />
margin:0 0 15px 0;<br />
padding:0;<br />
}<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
ul.sidelink li {<br />
list-style:none;<br />
margin:0 0 3px;<br />
padding:0;<br />
}<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
display:block;<br />
margin:5px 10px 5px 0;<br />
padding:3px 4px 3px 8px;<br />
text-align:left; width:140px;<br />
}<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
<br />
#footer {<br />
background:#fff;<br />
border-top:1px solid #d8d8d8;<br />
clear:both;<br />
margin:0;<br />
padding:0;<br />
}<br />
#footer a {<br />
color:gray;<br />
}<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
#footer p {<br />
color:gray;<br />
font-size:1.1em;<br />
line-height:1.3em;<br />
margin:15px 0 0;<br />
padding:0;<br />
}<br />
<br />
<br />
/* Additional classes */<br />
<br />
.photo {<br />
background-color:#f0f0f0;<br />
border:1px solid #d8d8d8;<br />
margin:0 0 15px;<br />
padding:2px;<br />
}<br />
.right {<br />
margin:-8px 0 8px 0;<br />
text-align:right;<br />
}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {<br />
display:none;<br />
color: #000;<br />
}<br />
<br />
/*Tabbed Panels*/<br />
<br />
#maintabs {<br />
clear:both;<br />
margin-top: 10px;<br />
}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
margin-top: 15px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#333;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activemaintab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
#prezi {<br />
width: 300px;<br />
height: 200px;<br />
background-color: #036;<br />
clear: both;<br />
margin-bottom: 10px;<br />
padding-bottom: 15px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {<br />
color: #oc9<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3><br />
</br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<br />
<div id="maintabs"><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Overview</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Circuit Design</h3><br />
<p>To begin construction of this plasmid, the DNA sequence was generated and edited in ApE, a DNA editing software available through the University of Utah, from several existing plasmid sequences available through other labs on the Penn State campus. The construct was rationally designed to include two start codons within 7 nucleotides of each other, and thus in different open reading frames. <p> Each codon, labeled RFP-coding ATG and sfGFP-coding ATG for simplicity, codes for a different reporter in the respective reading frame of each start codon. The first start codon, RFP-coding ATG, if recognized by the ribosome, will translate the reporter RFP. The second start codon, sfGFP-codon ATG, will translate the reporter sfGFP if recognized by the ribosome. Super-folder GFP (sfGFP) is used to allow for the nonsense codons of the out-of-frame RFP sequence preceding the sfGFP reporter which would inevitably be translated prior to the green reporter sequence should the ribosome recognize the sfGFP-coding ATG. The super-folder protein will fold regardless of this nonsense sequence, facilitating the measurement of codon slippage through the measurement of fluorescence</p><br />
<p>To prevent premature termination of the translation of either reporter, all stop codons in the respective reading frames of each codon were eliminated through amino acid sequence substitution.</p><br />
<h3>Circuit Construction</h3><br />
<p>Initially, 4 gBlocks, available through IDT as oligos with overlapping 40bp sequences complimentary to the adjacent oligo, were used to perform a Gibson Assembly reaction with a digested dRBS1 vector. After several failed attempts, a second approach was implemented. Oligos for the missing circuit sequence were designed and annealed, according to their complimentary 20bp overlaps, and inserted into the nearly-complete vector using Gibson Assembly. This attempt was successful, and the initial construct was verified with sequencing.</P> <br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons
Team:Penn State/Multiple Start Codons
2012-10-27T03:04:38Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
<br />
body {<br />
background:#e0e0e0;<br />
color:#303030;<br />
font:76% tahoma, verdana, sans-serif;<br />
margin:0;<br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {<br />
border:none;<br />
color:#505050;<br />
}<br />
#logo h1 a:hover {<br />
border:none;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
#logo p {<br />
font-size:1.3em;<br />
color:#505050;<br />
margin:0 0 20px 0;<br />
}<br />
<br />
<br />
/* Tags */<br />
<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {<br />
border-bottom:1px solid #505050;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
a img {<br />
border:0;<br />
}<br />
h1 {<br />
font-size:2.2em;<br />
font-weight:400;<br />
margin:0 0 5px;<br />
padding:0;<br />
}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {<br />
font-size:1.4em;<br />
font-weight:400;<br />
margin:0 0 10px;<br />
}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {<br />
margin:0 0 15px 15px;<br />
padding:0;<br />
}<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
<br />
#navitabs {<br />
clear:both;<br />
}<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
border-left:1px solid #fff;<br />
font-size:1.1em;<br />
margin:0 0 4px;<br />
padding:4px 8px;<br />
text-decoration:none;<br />
}<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#fff;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activenavitab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Main content */<br />
<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
<br />
#sidebar {<br />
float:right;<br />
width:150px;<br />
}<br />
#sidebar p {<br />
font-size:0.9em;<br />
line-height:1.3em;<br />
margin:0 0 15px;<br />
}<br />
#sidebar ul {<br />
margin:0 0 15px 0;<br />
padding:0;<br />
}<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
ul.sidelink li {<br />
list-style:none;<br />
margin:0 0 3px;<br />
padding:0;<br />
}<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
display:block;<br />
margin:5px 10px 5px 0;<br />
padding:3px 4px 3px 8px;<br />
text-align:left; width:140px;<br />
}<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
<br />
#footer {<br />
background:#fff;<br />
border-top:1px solid #d8d8d8;<br />
clear:both;<br />
margin:0;<br />
padding:0;<br />
}<br />
#footer a {<br />
color:gray;<br />
}<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
#footer p {<br />
color:gray;<br />
font-size:1.1em;<br />
line-height:1.3em;<br />
margin:15px 0 0;<br />
padding:0;<br />
}<br />
<br />
<br />
/* Additional classes */<br />
<br />
.photo {<br />
background-color:#f0f0f0;<br />
border:1px solid #d8d8d8;<br />
margin:0 0 15px;<br />
padding:2px;<br />
}<br />
.right {<br />
margin:-8px 0 8px 0;<br />
text-align:right;<br />
}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {<br />
display:none;<br />
color: #000;<br />
}<br />
<br />
/*Tabbed Panels*/<br />
<br />
#maintabs {<br />
clear:both;<br />
margin-top: 10px;<br />
}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
margin-top: 15px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#333;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activemaintab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
<br />
/* Header description area */<br />
<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
#prezi {<br />
width: 300px;<br />
height: 200px;<br />
background-color: #036;<br />
clear: both;<br />
margin-bottom: 10px;<br />
padding-bottom: 15px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {<br />
color: #oc9<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3><br />
</br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<br />
<div id="maintabs"><br />
<a class="activemaintab" href="href="https://2012.igem.org/Team:Penn_State/MSC_Multiple_Start_Codons">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Background</h3><br />
<p>mRNA is the molecule that carries information about the sequence of amino acids in a protein. However, much like the lines on a sheet of paper, the protein coding sequence of an mRNA molecule does not start right at the beginning, or top of the page. Instead, once the mRNA is bound by a ribosome, a start codon must first be read before the protein can be translated. This start codon is generally AUG, or Methionine.<br />
</p><br />
<h3>The Problem</h3><br />
<p>Once this start codon in read the ribosome will continue reading and building the polypeptide (protein) until a stop codon is reached. But what happens if you have two AUG codons close together? That is the question we are attempting to answer.</p> <br />
<p>We are trying to understand what happens when there are two start codons very close together, but out of frame. Out of frame refers to how the ribosome reads the mRNA. Remember those codons and how they are groups of three bases on the mRNA? The reading frame refers to which group of three. If you start at one base and read the bases in groups of three from that point on, that is one frame of reference. If you then move your start point ahead one base, then you are reading in a new reading frame. If you advance you starting point one more base, that is the third reading frame. If you advance it again you are now back in your first reading frame, but you have skipped the first codon. We are looking into what happens when you have multiple start codons close together, but in different reading frames. Which frame will be preferred?</p><br />
<h3>The Objective</h3><br />
<p>This construct aims to test the possibility of multiple start codons on a single mRNA strand. A plasmid has been engineered with two start codons slightly out of frame from each other, but close enough to test start codon slippage. Testing the fluorescence of E. coli carrying the plasmid will determine the rate of codon slippage</p><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Human_Practices
Team:Penn State/Human Practices
2012-10-27T03:04:02Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
margin-top: -49px;<br />
}<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0<br />
background-image: url(https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg);<br />
width: 150px;<br />
height: 100px;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {<br />
color: #666;<br />
}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;<br />
margin-top: -100px;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling<br />
">Main Results</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Human Practices</h3></br><br />
<p>Investigating the most basic theories of biology is not only important to laboratory research, but applies to students as well. Through the distribution of a molecular biology animation and the presentation of our research to high school students, the Penn State iGEM team has reached out to the community with its foundational research.</p><br />
<p class="right"><a href="http://www.youtube.com/watch?v=j_3I6E_W1l8&feature=youtu.be">Basics of Molecular Biology Animation >></a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Human Practices</h2><br />
<h3>State College High School Presentation</h3><br />
<p>Questioning the Central Dogma of Molecular Biology takes a foundational approach to the most strongly-held beliefs in the field. As we designed our projects, the Penn State team realized that this research applied not only to current researchers, but to those who were just beginning to learn the basic theories that govern life as we know it. High school students are taught to the Central Dogma, and accept it as true without a second thought. Showing them another side of molecular biology would provide an interesting counterpoint to their book-taught knowledge.</p><br />
<br/>The Penn State iGEM team visited a local high school and presented our designs and project objectives to a high school biology class. We began the seminar with a preview of the Basics of Molecular Biology animation compiled during the summer. Afterward, we presented our individual projects, stressing the importance of investigating outliers in a scientific field. The students were attentive, engaged, and asked excellent questions. Though the Penn State team may have introduced a shameless plug for the iGEM high school division, the experience was enlightening for both parties.</p><br />
<br/><br />
<hr><br />
<br/><br />
<h3>Animation and Prezi Distribution</h3><br />
<p>A cartoon animation of the basics of synthetic and molecular biology was developed and produced by one of our team members, Kevin Thyne. The Blender animation has been uploaded to YouTube, along with three Prezi animations describing the individual project designs and results. Both the animation and the Prezis are publically available, and all were shown at the State College High School presentation</p><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="images/test.jpg" height="100" width="130" alt="Team Picture Gallery" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Judging_Criteria
Team:Penn State/Judging Criteria
2012-10-27T03:03:42Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
margin-top: -49px;<br />
}<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0<br />
background-image: url(https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg);<br />
width: 150px;<br />
height: 100px;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {<br />
color: #666;<br />
}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em;<br />
font-weight:400;<br />
margin:0 0 10px;<br />
}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;<br />
margin-top: -100px;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Questioning Central Dogma</h3></br><br />
<p>Check out the progress we've made in the iGEM competition by reviewing the judging criteria we have met so far. The iGEM East Americas Jamboree awarded us a gold medal, but here is the proof how much we have achieved.</p><br />
<p class="right"><a href="https://2012.igem.org/Team:Penn_State/Project">Projects >></a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Judging Criteria</h2><br />
<br/><br />
<h2>Bronze Medal</h2><br />
<dl><br />
<dt>Team Registration </dt><br />
<dd><i>Complete</i></dd><br />
<dt>Team Wiki </dt><br />
<dd><i>Complete</i></dd><br />
<dt>Complete a Poster and Talk at the Jamboree</dt><br />
<dd><i>Complete</i></dd><br />
<dt>Submit a new BioBrick Part</dt><br />
<dd><i>Complete</i></dd><br />
</dl><br />
<h2>Silver Medal</h2><br />
<dl><br />
<dt>Demonstrate that a new BioBrick of the team's design worked as expected</dt><br />
<dd><i>Complete</i></dd><br />
<dt>Characterize the operation of a new BioBrick of the team's design and enter this information</dt><br />
<dd><i>Complete</i></dd><br />
</dl><br />
<h2>Gold Medal</h2><br />
<dl><br />
<dt>Improve the function of an existing BioBrick and post the information to the registry</dt><br />
<dd><i>Pending</i></dd><br />
<dt>Help another iGEM team</dt><br />
<dd><i>Incomplete</i></dd><br />
<dt>Outline and detail a new approach to Human Practices in Synthetic Biology as it relates to the team's project</dt><br />
<dd><i>Complete</i></dd><br />
</dl><br />
<br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Other
Team:Penn State/Other
2012-10-27T03:03:18Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
margin-top: -49px;<br />
}<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0<br />
background-image: url(https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg);<br />
width: 150px;<br />
height: 100px;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {<br />
color: #666;<br />
}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:18px; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;<br />
margin-top: -100px;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-size: 16px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling<br />
">Main Results</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Safety">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Safety, Attribution, and More!</h3></br><br />
<p>This page is for safety questions, attributions, collaboration, and contact information. Check out the menu below to jump to our completed Safety Questions, Collaboration with other iGEM teams, and Attributions to our generous sponsors and supporters. If you have any questions, please shoot us an email or give us a call!</p><br />
<p class="right"><a href="#">Contact Information</a> &raquo;</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Safety Questions</h2><br />
<p><h3>Would any of your project ideas raise safety issues in terms of research safety, public safety, or environmental safety?</h3><br />
<br/><br />
<p><br />
There are potential safety concerns with our project, but they do not exceed the normal caution exercised when working with antibiotic-resistant organisms. Although the team is working with a lab strain of E. coli that must be provided with the proper nutrients to survive, we are also working with a variety of antibiotic-resistant plasmids. While we follow proper sterile protocol in our work in the lab, there is still a chance that a wild pathogenic bacteria may enter reactions. In this event, a wild bacteria may have the chance to acquire an antibiotic resistant plasmid. If it were to be removed from the lab, this bacteria could exchange this resistance factor with other bacteria.</p><br />
<p> We do not work with virulent or potentially hazardous strains of bacteria in the lab, and thus would not risk infection or illness from these antibiotic-resistant bacteria. The only hazard is in allowing these antibiotic-resistant bacteria to transfer their plasmids to pathogenic bacteria outside the lab. Here there is the possibility of danger to the public or the environment depending on the strain of bacteria. </p><br />
<p><br />
Since some of our project ideas require the use of specific antibiotics, their use and disposal must be taken into consideration. Some of these compounds are toxic or even carcinogenic to humans and must be handled appropriately. These compounds may also be toxic to the environment or public if they were to not be disposed of properly. <br />
</p><br />
<br/><br />
<br/><br />
<br />
<h3>Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</h3><br />
<br/><br />
<p><br />
None of our BioBrick components raise any safety issues other than the antibiotic resistance they carry. Steps must be taken to ensure the antibiotic resistance is not accidentally imparted to a pathogen bacterial strain. Otherwise all of the BioBricks created by the team are for analytical purposes only and can only produce fluorescent proteins when correctly used.<br />
</p><br />
<br/><br />
<br/><br />
<br />
<h3>Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project?</h3><br />
<br/><br />
<p><br />
Yes, there is a biological safety review board at Penn State University. The Institutional Biosafety Committee (IBC) records, reviews, and approves laboratory research based on the NIH Guidelines for Research Involving Recombinant DNA Molecules. They have approved the research conducted in our lab, and we are in full compliance with the biosafety regulations imposed on our work by the IBC. <br />
</p><br />
<br/><br />
<br/><br />
<h3>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h3><br />
<br/><br />
<p><br />
Another way to ensure safety could be to create a new lab strain of E.coli that has more auxotrophic requirements. This would make it more difficult for a modified bacteria to escape and possibly spread a Resistance factor or Virulence factor.<br />
</p><br />
<p><br />
Parts could be made safer by introducing more regulation components. By making the bacteria require cofactors to function or to live in an antibiotic containing environment would help to ensure containment of the strain being studied. You could also make it more difficult for the new BioBricks to be transmitted in an active form to an undesired bacterial strain. <br />
</p><br/><br />
<hr><br />
<h2>Collaboration</h2><br />
<p> This year, the Penn State iGEM team completed a survey for the Macquarie Australia team, commenting on the open source nature of the iGEM competition wikis. We were able to provide detailed responses to questions about collaboration practices and open source material.</p><br />
<br/><br />
<hr><br />
<h2>Attributions</h2><br />
Due to the foundational nature of this year's project objectives, the Penn State iGEM team used several BioBrick promoter pieces its constructs. Therefore, all the non-synthesized genetic material was obtained from the Salis and Richard labs at Penn State.</p> <br />
<p></p><br />
<p>Using vectors from the Salis lab, both the multiple start codon and bidirectional promoter projects were assembled. </p><br />
<p>In addition to using plasmid vectors and equipment, the Salis lab provides a <a href="https://salis.psu.edu/software">RBS Library Calculator</a> software program to the public, which both forward- and reverse-engineers RBS sequences and translation initiation rates. This software was used heavily in the design of the multiple start codon project in synthesizing varying RBS lengths and strengths for the construct. <br />
<p>The codon optimization portion of the project was a side project of one of the Richard lab's graduate students, Michael Speer. His partially-assembled construct was the basis testing codon bias.</p><br />
<p>IDT, or <a href="http://www.idtdna.com/site">Integrated DNA Technologies</a>, synthesized the majority of small oligonucleotides for the Penn State iGEM team. The codon optimization repeat-sequences and varying RBS length and strength sequence variations were annealed from IDT's ssDNA oligos.</p><br />
<p>Since Penn State did not extensively collaborate with other iGEM teams this year, most parts were locally obtained, or synthesized off-campus.</p><br />
<h2>Sponsors</h2><br />
<br />
<br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Other
Team:Penn State/Other
2012-10-27T03:02:46Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
margin-top: -49px;<br />
}<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0<br />
background-image: url(https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg);<br />
width: 150px;<br />
height: 100px;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {<br />
color: #666;<br />
}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:18px; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;<br />
margin-top: -100px;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-size: 16px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Main Results<br />
">Modeling</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Safety">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Safety, Attribution, and More!</h3></br><br />
<p>This page is for safety questions, attributions, collaboration, and contact information. Check out the menu below to jump to our completed Safety Questions, Collaboration with other iGEM teams, and Attributions to our generous sponsors and supporters. If you have any questions, please shoot us an email or give us a call!</p><br />
<p class="right"><a href="#">Contact Information</a> &raquo;</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Safety Questions</h2><br />
<p><h3>Would any of your project ideas raise safety issues in terms of research safety, public safety, or environmental safety?</h3><br />
<br/><br />
<p><br />
There are potential safety concerns with our project, but they do not exceed the normal caution exercised when working with antibiotic-resistant organisms. Although the team is working with a lab strain of E. coli that must be provided with the proper nutrients to survive, we are also working with a variety of antibiotic-resistant plasmids. While we follow proper sterile protocol in our work in the lab, there is still a chance that a wild pathogenic bacteria may enter reactions. In this event, a wild bacteria may have the chance to acquire an antibiotic resistant plasmid. If it were to be removed from the lab, this bacteria could exchange this resistance factor with other bacteria.</p><br />
<p> We do not work with virulent or potentially hazardous strains of bacteria in the lab, and thus would not risk infection or illness from these antibiotic-resistant bacteria. The only hazard is in allowing these antibiotic-resistant bacteria to transfer their plasmids to pathogenic bacteria outside the lab. Here there is the possibility of danger to the public or the environment depending on the strain of bacteria. </p><br />
<p><br />
Since some of our project ideas require the use of specific antibiotics, their use and disposal must be taken into consideration. Some of these compounds are toxic or even carcinogenic to humans and must be handled appropriately. These compounds may also be toxic to the environment or public if they were to not be disposed of properly. <br />
</p><br />
<br/><br />
<br/><br />
<br />
<h3>Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</h3><br />
<br/><br />
<p><br />
None of our BioBrick components raise any safety issues other than the antibiotic resistance they carry. Steps must be taken to ensure the antibiotic resistance is not accidentally imparted to a pathogen bacterial strain. Otherwise all of the BioBricks created by the team are for analytical purposes only and can only produce fluorescent proteins when correctly used.<br />
</p><br />
<br/><br />
<br/><br />
<br />
<h3>Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project?</h3><br />
<br/><br />
<p><br />
Yes, there is a biological safety review board at Penn State University. The Institutional Biosafety Committee (IBC) records, reviews, and approves laboratory research based on the NIH Guidelines for Research Involving Recombinant DNA Molecules. They have approved the research conducted in our lab, and we are in full compliance with the biosafety regulations imposed on our work by the IBC. <br />
</p><br />
<br/><br />
<br/><br />
<h3>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h3><br />
<br/><br />
<p><br />
Another way to ensure safety could be to create a new lab strain of E.coli that has more auxotrophic requirements. This would make it more difficult for a modified bacteria to escape and possibly spread a Resistance factor or Virulence factor.<br />
</p><br />
<p><br />
Parts could be made safer by introducing more regulation components. By making the bacteria require cofactors to function or to live in an antibiotic containing environment would help to ensure containment of the strain being studied. You could also make it more difficult for the new BioBricks to be transmitted in an active form to an undesired bacterial strain. <br />
</p><br/><br />
<hr><br />
<h2>Collaboration</h2><br />
<p> This year, the Penn State iGEM team completed a survey for the Macquarie Australia team, commenting on the open source nature of the iGEM competition wikis. We were able to provide detailed responses to questions about collaboration practices and open source material.</p><br />
<br/><br />
<hr><br />
<h2>Attributions</h2><br />
Due to the foundational nature of this year's project objectives, the Penn State iGEM team used several BioBrick promoter pieces its constructs. Therefore, all the non-synthesized genetic material was obtained from the Salis and Richard labs at Penn State.</p> <br />
<p></p><br />
<p>Using vectors from the Salis lab, both the multiple start codon and bidirectional promoter projects were assembled. </p><br />
<p>In addition to using plasmid vectors and equipment, the Salis lab provides a <a href="https://salis.psu.edu/software">RBS Library Calculator</a> software program to the public, which both forward- and reverse-engineers RBS sequences and translation initiation rates. This software was used heavily in the design of the multiple start codon project in synthesizing varying RBS lengths and strengths for the construct. <br />
<p>The codon optimization portion of the project was a side project of one of the Richard lab's graduate students, Michael Speer. His partially-assembled construct was the basis testing codon bias.</p><br />
<p>IDT, or <a href="http://www.idtdna.com/site">Integrated DNA Technologies</a>, synthesized the majority of small oligonucleotides for the Penn State iGEM team. The codon optimization repeat-sequences and varying RBS length and strength sequence variations were annealed from IDT's ssDNA oligos.</p><br />
<p>Since Penn State did not extensively collaborate with other iGEM teams this year, most parts were locally obtained, or synthesized off-campus.</p><br />
<h2>Sponsors</h2><br />
<br />
<br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Notebook
Team:Penn State/Notebook
2012-10-27T03:02:26Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Penn State iGEM Notebook</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; <br />
font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666;}<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 5px;<br />
padding-right: 5px<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; <br />
display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
.Entries {<br />
background:#fff;<br />
color:#666; <br />
font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding-left:5px;<br />
width: 480px;<br />
float: left;<br />
}<br />
<br />
.Navigation {<br />
float: left;<br />
background-color: #f0f0f0;<br />
padding: 5px;<br />
width: 90px;<br />
margin-right: 15px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div> <br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Notebook</h3><br />
</br><br />
<p>These are our day-to-day lab activities. Here you can see our successes, failures, and alterations <br />
in our plans as we continue to gain hands-on experience in the cutting-edge field of synthetic biology. The journey was long an arduous, but there is a light at the end of the tunnel, and that feeling when months of work pay off is worth every minute of disappointment. </p><br />
</div><br />
<div id="main"><br />
<h2>Notebook</h2><br />
<br />
<a name="Top"> </a><br />
<div class="Navigation"><br />
<b> Entry Shortcuts</b><br/><br />
<ul><br />
<li><a href="#Week 1">Week 1</a> <br/> </li><br />
<li><a href="#Week 2">Week 2</a> <br/> </li><br />
<li><a href="#Week 3">Week 3</a> <br/> </li><br />
<li><a href="#Week 4">Week 4</a> <br/> </li><br />
<li><a href="#Week 5">Week 5</a> <br/> </li><br />
<li><a href="#Week 6">Week 6</a> <br/> </li><br />
<li><a href="#Week 7">Week 7</a> <br/> </li><br />
<li><a href="#Week 8">Week 8</a> <br/> </li><br />
<li><a href="#Week 9">Week 9</a> <br/> </li><br />
<li><a href="#Week 10">Week 10</a> <br/> </li><br />
<li><a href="#Week 11">Week 11</a> <br/> </li><br />
<li><a href="#Week 12">Week 12</a> <br/> </li><br />
<br />
</ul><br />
</div><br />
<br />
<br />
<br />
<div class="Entries"><br />
<p><br />
<a name="Week 1"></a><br />
<br />
<p><b>Monday, May 22, 2012</b><br/><br />
We prepared ampicillin and streptomycin plates in preparation for production of plasmids and competent cells. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Tuesday, May 23, 2012</b><br/><br />
Inoculation of LB broth with DH10B E. coli strain to prepare master mix/culture of cells in preparation for making chemically competent cells. These cells were incubated throughout the day and prepared for competency by the end of the day. Promotes were also planned out for the Multiple Promoters Project <!-- href --> and ordered. These promoters were designed to combine some of the components of the plasmid.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Wednesday, May 24, 2012</b><br/><br />
Biobricks were hydrated and transformed into Chemically Competent cells through heat shock (<br />
<a href="http://partsregistry.org/Part:BBa_R0063"; title="BBa_R0063";> BBa_R0063</a>, <br />
<a href="http://partsregistry.org/Part:BBa_R1062"; title="BBa_R1062";> BBa_R1062</a>, <br />
<a href="http://partsregistry.org/Part:BBa_R0078"; title="BBa_R0078";> BBa_R0078</a>, <br />
<a href="http://partsregistry.org/Part:BBa_I1051"; title="BBa_I1051";> BBa_I1051</a>, <br />
<a href="http://partsregistry.org/Part:BBa_R0079"; title="BBa_R0079";> BBa_R0079</a>, <br />
<a href="http://partsregistry.org/Part:BBa_R0071"; title="BBa_R0071";> BBa_R0071</a><br />
). LOB broth was also prepared for the recovery from the transformation. These cells were then plated to produce isolated colonies.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Thursday, May 25, 2012</b><br/><br />
Additional Biobricks were hydrated and transformed into Chemically Competent cells through heat shock (<br />
<a href="http://partsregistry.org/Part:BBa_I712074"; title="BBa_I712074"> BBa_I712074</a>,<br />
<a href="http://partsregistry.org/Part:BBa_R0062"; title="BBa_R0062"> BBa_R0062</a>,<br />
<a href="http://partsregistry.org/Part:BBa_I746104"; title="BBa_I746104"> BBa_I746104</a>,<br />
<a href="http://partsregistry.org/Part:BBa_I719005"; title="BBa_I719005"> BBa_I719005</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23119"; title="BBa_J23119"> BBa_J23119</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23100"; title="BBa_J23100"> BBa_J23100</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23101"; title="BBa_J23101"> BBa_J23101</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23103"; title="BBa_J23103"> BBa_J23103</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23104"; title="BBa_J23104"> BBa_J23104</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23106"; title="BBa_J23106"> BBa_J23106</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23107"; title="BBa_J23107"> BBa_J23107</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23110"; title="BBa_J23110"> BBa_J23110</a><br />
). LOB broth was used to help the cells recover from their transformation. More agar plates were poured. The Transformed cells form yesterday yielded isolated colonies. A single colony from each plate was used to create a broth in preparation for mini-prep of the plasmids. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Friday, May 25, 2012</b><br/><br />
Miniprep was done to extract the first six plasmids we transformed <ul><br />
<li><a href="http://partsregistry.org/Part:BBa_R0063"; title="BBa_R0063";> BBa_R0063</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_R1062"; title="BBa_R1062";> BBa_R1062</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_R0078"; title="BBa_R0078";> BBa_R0078</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_I1051"; title="BBa_I1051";> BBa_I1051</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_R0079"; title="BBa_R0079";> BBa_R0079</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_R0071"; title="BBa_R0071";> BBa_R0071</a></li> <br />
<br />
</ul><br />
<p> Colonies that grew from the transformation of our last 14 plasmids were cultured for later plasmid extraction:</p><ul><br />
<li><a href="http://partsregistry.org/Part:BBa_I712074"; title="BBa_I712074"> BBa_I712074</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_R0062"; title="BBa_R0062"> BBa_R0062</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_I746104"; title="BBa_I746104"> BBa_I746104</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_I719005"; title="BBa_I719005"> BBa_I719005</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23119"; title="BBa_J23119"> BBa_J23119</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23100"; title="BBa_J23100"> BBa_J23100</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23101"; title="BBa_J23101"> BBa_J23101</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23103"; title="BBa_J23103"> BBa_J23103</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23104"; title="BBa_J23104"> BBa_J23104</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23106"; title="BBa_J23106"> BBa_J23106</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23107"; title="BBa_J23107"> BBa_J23107</a></li> <br />
<li><a href="http://partsregistry.org/Part:BBa_J23110"; title="BBa_J23110"> BBa_J23110</a></li> <br />
</ul> <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Saturday, May 26, 2012</b><br/><br />
Hannah and Kevin extracted the plasmids from the 14 colonies that were cultured yesterday. Two of the BioBricks that needed to be retransformed (<br />
<a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a>,<br />
<a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a>)<br />
had isolated colonies on their plates and were cultured by Kevin for plasmid extraction the next day.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br/><br />
<p><br />
<a name="Week 2"><br />
<font style="font-weight:bold;" size="3">Week 2</font><br />
</a><br />
</p><br />
<br />
<p><br />
<b>Sunday, May 27, 2012</b><br/><br />
Hannah and Kevin extracted the two cultures that Kevin prepared yesterday. These plasmids were put on ice for concentration analysis later with the other extracted plasmids.<br />
<!--Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Monday, May 28, 2012</b><br/><br />
Memorial Day<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Tuesday, May 29, 2012</b><br/><br />
The 20 plasmids that have been grow during the previous week and weekend were digested by Hannah, Victoria, and Kevin. The plasmids were run in a gel, however errors in the gel's preparation resulted in a poor run. Always make sure your gasket is correctly seated before you pour the agarose!<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Wednesday, May 30, 2012</b><br/><br />
Plasmids for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a> were transformed and cultured by Hannah and Kevin. Chris, Kait, and Victoria re-transformed 10 plasmids and cultured those. A gel was run to confirm our plasmids and fragments but was inconclusive due to low concentration.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Thursday, May 31, 2012</b><br/><br />
Fragments for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoter Project</a> were digested and run through PCR by Hannah and Kevin. Kait and Victoria re-transformed the remaining 10 plasmids and 1 from yesterday that did not yield colonies. Kait and Victoria ran a gel to confirm a plasmid they could use in the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a>. <br/><br />
The originally transformed promoters were disposed of. Some of the cultures became a purple color. After centrifuging it was shown that the bacteria themselves were purple. This may be from contamination, so to be on the safe side, these plasmids were re-transformed. This may be an artifact from the plasmids themselves, but there is no consistent pattern. Very strange indeed! Always remember to use good sterile techniques when working with media and cultures!<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<br />
<p><br />
<b>Friday, June 1, 2012</b><br/><br />
Today Hannah digested components for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 3 : Multiple Start Codons">Multiple Start Codon Project</a>. Later she ran a gel and extracted the components for later use. Kait and Victoria prepared a plasmid for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a>. Kevin and Chris began work on a plasmid backbone and fluorescent component of the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>. <br/><br />
The team also received a new gel illuminator to see their bands without the use of UV light. They were very excited. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 3"><br />
<font style="font-weight:bold;" size="3">Week 3</font><br />
</a><br />
</p><br />
<p><br />
<b>Monday, June 4, 2012</b><br/><br />
Hannah hydrated <a href="http://partsregistry.org/Part:BBa_I13521">BBa_I13521</a> and used it to gauge the competency of the chemically competent cells we prepared earlier. Victoria and Kait ran a gel to confirm some of the components they need for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a>. Kevin and Chris worked on some cleaning and housekeeping.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, June 5, 2012</b><br/><br />
Victoria and Kait completed a transformation for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a>. Kevin mini-prepped two plasmids for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>. Chris and Kevin then froze some cultures containing these plasmids for later use. Hannah completed a CBAR and transformation for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 3 : Multiple Start Codons">Multiple Start Codon Project</a>. She also tested the competency rate of the chemically competent cells the team prepared earlier.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, June 6, 2012</b><br/><br />
Kevin and Chris ran a gel to separate components for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>'s final construct. Victoria and Kait inoculated cultures with isolated colonies. This culture has a plasmid that they will use for their final construct. Hannah is in the process of building a higher efficiency Chemically Competent DH10B cell line. The higher transformation rate will help not only the team but is critical for her CBAR assembly of her construct for the Multiple Start Codon Project. Hannah also started planning and development of a secondary construct for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a> to act as a control for the experiments. <br />
<!-- Sgt_Bisendi ---><br />
</p><br />
<p><br />
<b>Thursday, June 7, 2012</b><br/><br />
Kevin and Chris ran a gel to separate another component for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>. One of the component did not run well on the gel. This component was amplified again with PCR. Kait and Victoria continued work on the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a> with the digestion of their plasmid, and subsequent gel extraction of the specific components. Hannah continued work on designing the primers for the CBAR reaction to create the control construct for the Multidirectional Promoters Project. Hannah also prepped a culture in an attempt to get a stock culture of DH10B growing. Her goal is to create a higher efficiency Chemically Competent (CC) DH10B cell line to help her complete her CBAR for her <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 3 : Multiple Start Codons">Multiple Start Codon Project</a>. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, June 8, 2012</b><br/><br />
Kevin extracted components for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>. from the gel that was run yesterday. Hannah worked more on the design of the primers for one of the constructs for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>. Kevin completed a streak plate to create isolated colonies in preparation for the production of more Chemically Competent cells. Kait and Victoria continued their work on their construct for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a>. Victoria designed primers for their construct. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, June 9, 2012</b><br/><br />
Kevin and Chris produced a new set of Chemically Competent cells. Their goal is to create a cell line with a higher transformation efficiency. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 4"><br />
<font style="font-weight:bold;" size="3">Week 4</font><br />
</a><br />
</p><br />
<p><br />
<b>Monday, June 11, 2012</b><br/><br />
Victoria and Kait mini-prepped a plasmid for their final construct. Hannah and Victoria poured new chloramphenicol plates. Chris ran a digest on six plasmids for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>. Kevin and Hannah transformed six plasmids for this project as well. Kevin ran a transformation to test the competency of the cells that he and Chris prepared over the weekend. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, June 12, 2012</b><br/><br />
Hannah completed her CBAR transformation today. Kait, Victoria, and Alexander completed a gel, gel extraction, and transformation for constructs for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a>. Kevin and Chris inoculated six cultures with transformed colonies that grew overnight. Chris also did a comparison transformation between the CC DH10B cell line that he and Kevin made over the weekend, and the other CC cells that the team has been using. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, June 13, 2012</b><br/><br />
Victoria and Kait made more plasmid stock for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 1 : Codon Optimization">Codon Optimization Project</a>. Hannah made cultures from the colonies that grew from her CBAR reaction. Kevin and Chris extracted plasmids for the <a href="https://2012.igem.org/Team:Penn_State/Projects#Project 2 : Multidirectional Promoters">Multidirectional Promoters Project</a>. They also began making more plasmid vector in preparation for their CBAR reaction. <br />
<!--- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, June 14, 2012</b><br/><br />
Plasmids from Hannah's CBAR reaction were extracted and back-up cultures were inoculated. Victoria, Kait, and Alexander ran a gel to confirm the components of plasmids to be used in the final Codon Optimization Construct. Kevin and Chris inoculated a culture with a colony containing the vector plasmid for the Multidirectional Promoters Project. They came in later that night to extract the plasmid.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, June 15, 2012</b><br/><br />
Kevin ran a PCR on the vector component that he and Chris extracted the previous night. This was run through gel electrophoresis to separate the desired component from other side-products, and to ensure the fragment was the correct size. This was finally cleaned and concentrated for a CBAR reaction on Monday. Hannah grew her cultures from her CBAR reaction to get a higher plasmid yield. She also prepared new chemically competent DH10B cells since the stock was running low. Victoria, Kait, and Alexander checked a plasmid's components for the Codon Optimization Project. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, June 16, 2012</b><br/><br />
Hannah mini-prepped cultures for the Codon Optimization Project, and made TSS and Chemically Competent cells.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 5"><br />
<font style="font-weight:bold;" size="3">Week 5</font><br />
</a><br />
</p><br />
<p><br />
<b>Sunday, June 17, 2012</b><br/><br />
Hannah came in and transformed the new Chemically Competent cells to test their competency. She also swapped the broth in her CBAR cultures to facilitate better growth.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Monday, June 18, 2012</b><br/><br />
Victoria prepared and stored cultures containing plasmids for the Codon Optimization Project. Hannah and Kevin extracted plasmids from colonies from Hannah's CBAR reaction. These were then digested and run on a gel. Kevin and Chris ran a PCR to produce a component for the final construct for the Multidirectional Promoters Project. The first reaction did not yield the expected results. Two more were prepared for comparison. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, June 19, 2012</b><br/><br />
Hannah and Kevin extracted plasmids from the CBAR cultures again to get a higher yield for sequencing. Victoria and Alexander worked on the Codon Optimization construct by culturing insert components. They also extracted plasmids with the desired components for the final construct. These components were digested, run in a gel, extracted, and Cleaned and Concentrated. Kevin and Chris ran a gel to compare the PCR runs set up yesterday. This showed that the PCR runs had not yielded the desired components. Another PCR run was prepared and run a lower temperature for better annealing. Hannah ran a gel of her CBAR plasmid digests to see if they contained the correct parts. Unfortunately both gels showed that the plasmids were self ligations of a single component of the construct. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, June 20, 2012</b><br/><br />
Victoria developed a new method for the assembly of the Codon Optimization Project. Hannah designed primers for the main construct for the Multiple Start Codons project. Kevin and Chris ran gels and extracted components for the final construct for the Multidirectional Promoters Project. They also ran another set of PCR to make more of the three components for the CBAR assembly they will need to do. Kait and Alexander extracted plasmids containing components for the Codon Optimization Construct. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, June 21, 2012</b><br/><br />
Kait and Victoria sent a construct for sequencing. Hannah compared the effects of a more concentrated LB broth on the growth of DH10B. Kevin completed a storyboard for an animation describing one of the projects. He also isolated components from the PCR runs set up yesterday through the use of gel electrophoresis.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, June 22, 2012</b><br/><br />
Victoria, Kait, and Alexander annealed components for inserts for their project. Hannah also finished preparations for a new line of TSS CC DH10B. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, June 23, 2012</b><br/><br />
Kevin came in and ran a CBAR reaction on the components for the Multidirectional Promoters Project. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 6"><br />
<font style="font-weight:bold;" size="3">Week 6</font><br />
</a><br />
</p><br />
<br />
<p><br />
<b>Monday, June 25, 2012</b><br/><br />
Kevin transformed the CBAR product into DH10B and plated it. Hannah worked on developing a document that condenses all of the protocols used in the lab. Victoria worked to develop new primers for building the main construct for the Codon Optimization Project. Chris and Hannah began work on new Chemically Competent DH10B cells. Hannah completed PCR runs for her new primers that arrived today. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, June 26, 2012</b></br><br />
The transformation from the CBAR reaction Kevin did yesterday proved to be successful, and produced many colonies. Six of these were chose and cultured. They will be tested to see if they contain the complete construct for the Multidirectional Promoters Project. Victoria, Kait, and Alexander worked on ligating components for the Codon Optimization Project. They also grew cultures containing components they would need later on in the assembly. Hannah ran PCR on some new segments of her final construct that just came in. These were later run in a CBAR. This new set of components would be better and produce the desired construct. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, June 27, 2012</b><br/><br />
The cultures from the CBAR for the Multidirectional Promoters Project were mini-prepped and tested to see if they contained the correct components for the final construct. The gel to check this unfortunately did not set correctly and the cultures needed to be redone. Fortunately, Kevin had made a backup. Some additional colonies were also cultured to expand the search. Hannah worked on the sequencing primers for the Multidirectional Promoter construct. She also continued work on a document combining all of the protocols we use in the lab. Kait and Victoria completed digestions and ligations for the construction of their construct for the Codon Optimization Project. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, June 28, 2012</b><br/><br />
Cultures from the Multidirectional Promoters Project CBAR reaction were mini-prepped, again. 4 additional colonies were prepped to expand the search for the correct construct. These were then digested in preparation for use in a gel to confirm length. Hannah ran PCR on components for the Multiple Start Codon construct. She also inoculated cultures with colonies from one of her new CBAR reactions. Victoria, Kait, and Alexander worked on their own PCR runs to make the new Codon Optimization construct. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, June 29, 2012</b><br/><br />
Hannah ran another PCR for one of her new G-blocks. She then mini-prepped cultures from her CBAR reaction. Some of the colonies seemed to be red, indicating they had taken up a plasmid with at least some of the desired components. Kait and Alexander ran gels to digest components and PCR products in preparation for an assembly of the construct for the Codon Optimization Project.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, June 30, 2012</b><br/><br />
Hannah completed a digestion on her CBAR plasmids and ran a gel to confirm their length. Chris ran gels to determine which colonies to have sequenced from the CBAR reaction for the Multidirectional Promoters Project. Kevin completed the beginning of an animation for the team.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 7"><br />
<font style="font-weight:bold;" size="3">Week 7</font><br />
</a><br />
</p><br />
<p><br />
<b>Monday, July 2, 2012</b><br/><br />
Kevin completed another component for an animation. <!-- Achievement Unlocked: Milestone! --><br />
Hannah ran a gel to assess the length of the plasmids in the colonies she cultured from her CBAR reaction for the Multiple Start Codon Project. To test the stability of some of her plasmids, she transformed them back into DH10B cells. Chris grew up cultures with the desired colonies for sequencing to ensure there was enough sample. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, July 3, 2012</b><br/><br />
Chris mini-prepped cultures to get plasmids for sequencing. Hannah inspected her cultures for their RFP expression, which was present. She used these plates to culture broth in preparation for sequencing. Victoria and Kait looked through some of the teams stocks for primers that could be used for sequencing the construct for the Codon Optimization Project. Sequencing primers were acquired for the Multidirectional Promoters Project.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, July 4, 2012</b><br/><br />
Hannah inoculated cultures for the Multiple Start Codon Project. Victoria checked her plates for signs of expression of mCherry. The broth cultures for mini-prepping and sequencing still required another day of growth. Kevin made some progress with an animation for the team.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, July 5, 2012</b><br/><br />
Chris mini-prepped cultures for sequencing, but the yields were still low. Victoria, Kait, and Alexander mini-prepped their cultures in preparation for sequencing. Hannah also ran some mini-preps to prepare samples for sequencing. Kevin worked more on the animations and was shown a better way for making Chemically Competent cells. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, July 6, 2012</b><br/><br />
Samples were sent off for sequencing for the Codon Optimization Project and Multidirectional Promoters Project. Updates were also made to the website concerning the Navigation Bar.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, July 7, 2012</b><br/><br />
Kevin came into the lab to escape the heat and spent some time working on an animation for the team. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br />
<br/><br />
<p><br />
<a name="Week 8"><br />
<font style="font-weight:bold;" size="3">Week 8</font><br />
</a><br />
</p><br />
<p><br />
<b>Monday, July 9, 2012</b><br/><br />
Victoria, Kait, and Alexander ran digestions to check the length of some of their fragments. Chris check his cultures for growth, but they were not ready for mini-prepping yet. Kevin completed the first 93 seconds of an animation for the team. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, July 10, 2012</b>< br/><br />
Chris received his sequencing data. It looked promising, however parts of it seemed inconclusive. He cultured this strain in preparation for testing it by inserting a promoter. Victoria ran a PCR reaction for some of the components for the Codon Optimization Project. Victoria and Alexander worked to make master cultures of components for the Codon Optimization Project. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, July 11, 2012</b><br/><br />
Victoria, Kait, and Alexander mini-prepped cultures in preparation for sequencing. Chris transformed a plasmid, BBa_J23102, to use to test a construct that looks positive for having all the parts from the CBAR. Victoria also ran a digestion on the plasmids she prepared earlier to see if they possessed the correct restriction sites. The plasmids that looked promising were sent to sequencing. Kevin made more progress with the animation. He also worked with Nikki to help plan out the flow of the website.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, July 12, 2012</b><br/><br />
Chris checked his results from sequencing. With help from Hannah, he was able to conclude that there was a good chance that the plasmid was the desired construct. Hannah also checked her results from sequencing plasmids from her colonies from her second CBAR reaction. Her results showed to be negative, in response she ran another CBAR. Chris mini-prepped the J23102 that he grew up yesterday in preparation of inserting it into his suspected construct. Victoria ran a PCR for a fragment of the Codon Optimization Project, and cleaned the product of a previous PCR. Kevin made progress and created all new home and team pages. He also managed to get the navigation bar working, albeit in a crude format. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, July 13, 2012</b><br/><br />
Chris checked the concentration of some of his mini-preps in preparation for sequencing and ligations. Victoria transformed some plasmids to be able to check them later for the desired parts. Victoria worked with Jyotsna to find some of the needed parts for the Codon Optimization Project. Kevin updated some of the webpages and poured some plates.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 9"><br />
<font style="font-weight:bold;" size="3">Week 9</font><br />
</a><br />
</p><br />
<p><br />
<b>Monday, July 16, 2012</b><br/><br />
Chris ran some mini-preps for the Multidirectional Promoters Project. Victoria revved the results of her sequencing with Kait. Kevin worked with Hannah and taught her some HTML. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, July 17, 2012</b><br/><br />
Hannah came in early to mini-prep cultures from her most recent CBAR for the Multiple Start Codon Project. These were subsequently digested to check for proper length. The result of this gel raised some questions. The colonies that she had grown up were all expression RFP. There were some colonies that were expressing GFP, but these were not cultured. Analysis of the gel and expression of RFP indicates that those plasmids did not contain all of the correct components. The GFP colonies probably would contain the entire construct. These were cultured to test this hypothesis. Victoria worked with Jyotsna to design primers to make some of the necessary parts for the Codon Optimization Project construct. Alexander is also beginning presentations for describing all of the projects. Kevin made some headway with the animation, which is till humming along. He also prepared materials for making new Chemically Competent cells. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, July 18, 2012</b><br/><br />
Kevin updated some of the website and made some new chemically competent cells. Hannah and Kevin then transformed a number of fluorescent protein plasmids into these new cells. Hannah prepped her GFP presenting colonies from her CBAR. These were then digested to check them for length. The correct plasmids were prepped to be sent to sequencing. Victoria planned and designed primers for the Codon Optimization Project. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, July 19, 2012</b><br/><br />
Victoria ran a PCR of one of her components for the Codon Optimization Project. Hannah sent her plasmids to sequencing. Some of the plasmids that she transformed yesterday with Kevin were successful and presenting the correct fluorescent protein. Chris ran a gel on the construct for the Multidirectional Promoter Project. Kait and Victoria transformed some plasmids in preparation for another PCR to make a fragment for the Codon Optimization Project. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, July 20, 2012</b><br/><br />
Victoria and Alexander completed a ligation to hopefully give them their desired construct. Kevin created a new run of chemically competent cells for the lab. Hannah ran a PCR of one of the components of her construct. This was to test to see if the deletion in the construct was caused by the PCR or if the problem resided somewhere else. Victoria also completed a digestion of a plasmid to check its length. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, July 21, 2012</b><br/><br />
Hannah transformed and plated her partial construct to have it as a back-up to work from to insert the deleted piece. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a> <br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 10"><br />
<font style="font-weight:bold;" size="3">Week 10</font><br />
</a><br />
</p><br />
<p><br />
<b>Sunday, July 22, 2012</b><br/><br />
Hannah came in to make cultures from isolated colonies from the plates she made yesterday. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Monday, July 23, 2012</b><br/><br />
Chris completed mini-preps and digestions on components to be ligated together to test the suspected construct for the Multidirectional Promoter Project. Kevin worked more on the animation and got to another milestone. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, July 24, 2012</b><br/><br />
Hannah ran a PCR on the vector component for the Multiple Start Codon Project. She proceeded to complete another CBAR to try and get the missing segment into the construct. Kait and Victoria inserted a fragment into their vector backbone. This will hopefully yield the construct for the Codon Optimization Project. Kevin ran a gel extraction and cleanup on a gel run by Chris. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, July 25, 2012</b><br/><br />
Chris completed some annealings for inserts for his construct for the Bidirectional Promoter Project (formerly known as the Multidirectional Promoter Project). Kait and Victoria ran some miniprepps and plated some cultures to create stocks of their construct components for the Codon Optimization Project. Hannah learned more about working with webpages. Kevin made some more progress with the animation. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, July 26, 2012</b><br/><br />
Chris completed some transformation into new CC and old CC. This will not only show if his construct is correct, but also determine how good the new CC are. Hannah ran some transformations to see if she got the completed construct for the Multiple Start Codon Project. Victoria tracked down the required materials for running flow cytometry on samples when the Codon Optimization Construct is completed. Kevin made more headway with the animation. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, July 27, 2012</b><br/><br />
Kait completed 12 mini-preps of two components needed for one of the assembly methods for the Codon Optimization Project's construct. She also made some stock cultures for the freezer for long-term storage. She also picked some colonies and cultured them. Kevin continued work on the animation. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, July 28, 2012</b><br/><br />
Kevin came in to check on some transformations Chris had plated. Unfortunately, all of the plates showed no growth.<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a><br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 11"><br />
<font style="font-weight:bold;" size="3">Week 11</font><br />
</a><br />
</p><br />
<p><br />
<b>Sunday, July 29, 2012</b><br/><br />
Kait came in and plated some cultures for the Codon Optimization Project. These cultures will hopefully yield the desired functioning construct with the new promoter. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Monday, July 30, 2012</b><br/><br />
Kait completed a ligation and transformation for the Codon Optimization Project. Chris completed a number of mini-preps for the Bidirectional Promoter Project. Kevin made more progress with the animation. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Tuesday, July 31, 2012</b><br/><br />
Kait completed mini-preps for the Codon Optimization Project to create more stocks for later. Chris completed another transformation of his ligated component. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Wednesday, August 1, 2012</b><br/><br />
Kait completed a ligation and transformation using one of the other methods for synthesizing the desired construct for the Codon Optimization Project. Kevin completed some preparations for sequencing some colonies from a CBAR for the Multiple Start Codon Project. He also continued work on the animation. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Thursday, August 2, 2012</b><br/><br />
Kait and Chris both reran ligations and transformations after the identification of an error in the procedure. Kevin continued work on the animation and prepared more media for the team. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Friday, August 3, 2012</b><br/><br />
Kait completed a mini-prep for the Codon Optimization Project. Chris completed a six hour ligation. Kevin worked one the animation and prepped samples for sequencing for the Multiple Start Codon Project. He also ran tests to check the efficiency of the competent cells he made earlier. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<b>Saturday, August 4, 2012</b><br/><br />
Another milestone was reached in the animation. It is almost done!<br />
<!-- Sgt_Bisendi --><br />
</p><br />
<p><br />
<a href="#Top">Back to top.</a><br />
</p><br />
<br />
<br/><br />
<p><br />
<a name="Week 12"><br />
<font style="font-weight:bold;" size="3">Week 12</font><br />
</a><br />
</p><br />
<p><br />
<b>Sunday, August 5, 2012</b><br/><br />
Chris checked on his digestion from Friday, and completed a ligation. <br />
<!-- Sgt_Bisendi --><br />
</p><br />
</div><!--close Entries--><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html><br />
<br />
<br />
<!-- Instructions on posting to the Notebook<br />
1. Enter a space between the last entry and the new one you are entering.<br />
2. Open your entry with a <p> to make a new paragraph for you text.<br />
3. Enter a <b> The Date </b> tag to make the text bold for the date.<br />
4. Enter a <br/> after the date to crate a carriage return to the next line.<br />
5. Enter your text for the notebook.<br />
6. On the next new line enter </p> to end your paragraph and entry.<br />
7. Click the "Save Page" button at the bottom of the page to update the page and save it for everyone to see.<br />
8. Congratulations! You have just written code in HTML. Go have a cookie! <br />
--></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Modeling
Team:Penn State/Modeling
2012-10-27T03:01:55Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Main Results</h3><br />
<p>Included here are the pooled results of each project. All results are measurements of fluorescence performed by a TECANM1000 instrument. For detailed discussion, please see the individual <a href="href="https://2012.igem.org/Team:Penn_State/Project>project pages</a>. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>BiDirectional Promoter Project Data</h2><br />
<p>Here we can see some of the data collected for the Bidirectional Promoter Project. The data shows the fluorescence of GFP and RFP from different promoters collected by the TCAN.<br />
</p><br />
<p>GFP Data for all of the samples tested and the average of those samples for a given construct. The promoters tested so far are <a href="http://partsregistry.org/Part:BBa_J23102">BBa_J23102</a>, and three custom palindromic promoters. <br />
</p><br />
<a href="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg" width="575px" height="275px"><br />
</a><br />
<br/><br />
<a href="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg" width="380px" height="270px"><br />
</a><br />
<br/><br />
<p>RFP Data for all of the samples tested and the average of those samples is shown below.</p><br />
</p><br />
<a href="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg" width="575px" height="275px"><br />
</a><br />
<br/><br />
<a href="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg" width="380px" height="270px"><br />
</a><br />
<br />
<h2>Multiple Start Codon Project Data</h2><br />
<p>Here is some of the preliminary data for the MSC Project. The graph depicts the fluorescence measured by expression from each individual start codon.</p><br />
<a href="https://static.igem.org/mediawiki/2012/1/1e/PennState_MSC_Data_1.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/1/1e/PennState_MSC_Data_1.jpg" width="500px"><br />
</a><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Modeling
Team:Penn State/Modeling
2012-10-27T03:01:33Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Modeling and Results</h3><br />
<p>Included here are the pooled results of each project. All results are measurements of fluorescence performed by a TECANM1000 instrument. For detailed discussion, please see the individual <a href="href="https://2012.igem.org/Team:Penn_State/Project>project pages</a>. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>BiDirectional Promoter Project Data</h2><br />
<p>Here we can see some of the data collected for the Bidirectional Promoter Project. The data shows the fluorescence of GFP and RFP from different promoters collected by the TCAN.<br />
</p><br />
<p>GFP Data for all of the samples tested and the average of those samples for a given construct. The promoters tested so far are <a href="http://partsregistry.org/Part:BBa_J23102">BBa_J23102</a>, and three custom palindromic promoters. <br />
</p><br />
<a href="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg" width="575px" height="275px"><br />
</a><br />
<br/><br />
<a href="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg" width="380px" height="270px"><br />
</a><br />
<br/><br />
<p>RFP Data for all of the samples tested and the average of those samples is shown below.</p><br />
</p><br />
<a href="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg" width="575px" height="275px"><br />
</a><br />
<br/><br />
<a href="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg" width="380px" height="270px"><br />
</a><br />
<br />
<h2>Multiple Start Codon Project Data</h2><br />
<p>Here is some of the preliminary data for the MSC Project. The graph depicts the fluorescence measured by expression from each individual start codon.</p><br />
<a href="https://static.igem.org/mediawiki/2012/1/1e/PennState_MSC_Data_1.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012/1/1e/PennState_MSC_Data_1.jpg" width="500px"><br />
</a><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Parts
Team:Penn State/Parts
2012-10-27T03:01:10Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {#666;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
tr {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #333;<br />
}<br />
tr a {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #000;<br />
text-decoration: none;<br />
}<br />
tr a:hover {<br />
cursor: pointer;<br />
color: #066;<br />
font-family: Tahoma, Geneva, sans-serif;<br />
text-decoration: none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Parts Submitted to the Registry</h3><br />
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p><br />
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Submitted Parts</h2><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Name</th><br />
<th scope="col">Type</th><br />
<th scope="col">Description</th><br />
<th scope="col">Symbolic Model</th><br />
<th scope="col">Length (bp)</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td><br />
<td>device</td><br />
<td>multiple start codons coding for RFP and sfGFP respectively</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td><br />
<td>device</td><br />
<td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td><br />
<td>device</td><br />
<td>AHL leader with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</br><br />
<br />
<h2>Continuing Research</h2><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<br />
<br />
<tr><br />
<td>theonine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_1</td><br />
<td>complete and sequenced</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_2</td><br />
<td>complete and sequenced</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_3</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_alternating</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<br />
</table><br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters<br />
">Bidirectional Promoters</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23103</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23114</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>complete and sequenced</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
</table><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Project
Team:Penn State/Project
2012-10-27T03:00:47Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<br />
<title>Projects</title><br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030;<br />
font:76% tahoma, verdana, sans-serif;<br />
margin:0;<br />
padding:15px 0 0;<br />
}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {<br />
border:none;<br />
color:#505050;<br />
}<br />
#logo h1 a:hover {<br />
border:none;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
#logo p {<br />
font-size:1.3em;<br />
color:#505050;<br />
margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {<br />
border-bottom:1px solid #505050;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
a img {<br />
border:0;<br />
}<br />
a:visited {<br />
color: #oc9<br />
}<br />
h1 {<br />
font-size:2.2em;<br />
font-weight:400;<br />
margin:0 0 5px;<br />
padding:0;<br />
}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {<br />
font-size:1.4em;<br />
font-weight:400;<br />
margin:0 0 10px;<br />
}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {<br />
margin:0 0 15px 15px;<br />
padding:0;<br />
}<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
/* Navitabs menu */<br />
<br />
#navitabs {<br />
clear:both;<br />
}<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
border-left:1px solid #fff;<br />
font-size:1.1em; margin:0 0 4px;<br />
padding:4px 8px;<br />
text-decoration:none;<br />
}<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#fff; font-size:1.1em;<br />
margin:0 0 5px; padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activenavitab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
/* Main content */<br />
<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
/* Sidebar */<br />
<br />
#sidebar {<br />
float:right;<br />
width:150px;<br />
}<br />
#sidebar p {<br />
font-size:0.9em;<br />
line-height:1.3em;<br />
margin:0 0 15px;<br />
}<br />
#sidebar ul {<br />
margin:0 0 15px 0;<br />
padding:0;<br />
}<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
ul.sidelink li {<br />
list-style:none;<br />
margin:0 0 3px;<br />
padding:0;<br />
}<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
display:block;<br />
margin:5px 10px 5px 0;<br />
padding:3px 4px 3px 8px;<br />
text-align:left;<br />
width:140px;<br />
}<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
<br />
#footer {background:#fff;<br />
border-top:1px solid #d8d8d8;<br />
clear:both; margin:0;<br />
padding:0;<br />
}<br />
#footer a {<br />
color:gray;<br />
}<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
#footer p {<br />
color:gray;<br />
font-size:1.1em;<br />
line-height:1.3em;<br />
margin:15px 0 0;<br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
<br />
.photo {<br />
background-color:#f0f0f0;<br />
border:1px solid #d8d8d8;<br />
margin:0 0 15px;<br />
padding:2px;<br />
}<br />
.right {<br />
margin:-8px 0 8px 0;<br />
text-align:right;<br />
}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {<br />
color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {<br />
display:none;<br />
color: #000;<br />
}<br />
#boxLeft {<br />
background-color: #f0f0f0;<br />
text-align: left;<br />
float: left;<br />
height: 260px;<br />
width: 188px;<br />
margin-top: 10px;<br />
margin-right: 16px;<br />
margin-bottom: 10px;<br />
padding-bottom: 10px;<br />
margin-bottom: 10px;<br />
}<br />
#boxLeft h3 {<br />
font-family: Verdana, Geneva, sans-serif;<br />
font-size: 16px;<br />
color: #101010;<br />
background-color: #FF9;<br />
text-align: center;<br />
padding-top: 8px;<br />
padding-bottom: 8px;<br />
} <br />
#boxRight {<br />
background-color: #f0f0f0;<br />
text-align: left;<br />
float: left;<br />
height: 260px;<br />
width: 188px;<br />
margin-top: 10px;<br />
margin-right: 5px;<br />
padding-bottom: 10px;<br />
margin-bottom: 10px; <br />
}<br />
#boxRight h3 {<br />
font-family: Verdana, Geneva, sans-serif;<br />
font-size: 16px;<br />
color: #101010;<br />
background-color: #F99;<br />
text-align: center;<br />
padding-top: 8px;<br />
padding-bottom: 8px;<br />
} <br />
#boxCenter {<br />
background-color: #f0f0f0;<br />
text-align: left;<br />
float: left;<br />
height: 260px;<br />
width: 190px;<br />
margin-top: 10px;<br />
margin-right: 16px;<br />
margin-bottom: 10px;<br />
padding-bottom: 5px;<br />
}<br />
#boxCenter h3 {<br />
font-family: Verdana, Geneva, sans-serif;<br />
font-size: 15px;<br />
color: #101010;<br />
background-color: #39f;<br />
text-align: center;<br />
padding-top: 8px;<br />
padding-bottom: 8px;<br />
}<br />
#right {<br />
width:188px;<br />
}<br />
#center {<br />
width:188px;<br />
}<br />
#left {<br />
width:188px;<br />
}<br />
.readmore {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 12px;<br />
color: #333333;<br />
text-align: right;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
#photo {<br />
height: 150px;<br />
width: 150px;<br />
background-image: url(#a);<br />
background-color: #f0f0f0;<br />
margin-bottom: 20px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"></span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> </span> <br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"></span> <br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"></span> <br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"></span> <br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"></span> <br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a> </div><br />
<br />
<div id="desc"><br />
<h3>Team Projects</h3><br />
<br />
<p>Utilizing synthetic biology cloning techniques, the Penn State iGEM team has designed three genetic constructs that test the most basic assumptions of molecular biology. <br />
While each of the individual projects looks at a different aspect of the mantra, they all come together under the umbrella of Questioning the Central Dogma of Molecular Biology.</p><br />
<p class="right"><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts>></a></div><br />
<br />
<div id="main"><br />
<br />
<div id="boxLeft"><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons"><h3>Multiple Start Codons</h3></a><br />
<div id="left"><img src="https://static.igem.org/mediawiki/2012/2/23/Msc.jpg"></div> <br />
<p> <br />
<p> <br />
<p> <br />
<p class="block">mRNA strands should have only one recognized start codon. What happens when multiple start codons are introduced in translation?</p><br />
<p class="readmore"><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">read more...</a></p> <br />
</div><br />
<br />
<br />
<br />
<div id="boxCenter"><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters"><h3>Bidirectional Promoters</h3></a> <br />
<div id="center"><img src="https://static.igem.org/mediawiki/2012/e/e1/Bidir.jpg"></div> <br />
<p> <br />
<p> <br />
<p class="block">Promoters usually initiate translation in one direction. Which promoters promote both foward and reverse on the mRNA strand? <br />
<p class="readmore"><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">read more...</a></p><br />
</div><br />
<br />
<br />
<br />
<div id="boxRight"><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization"><h3>Codon Optimization</h3></a><br />
<div id="right"><img src="https://static.igem.org/mediawiki/2012/7/7a/Codopt.jpg"></div></p> <br />
<p> <br />
<p> <br />
<p> <br />
<p class="block">Each organism has a preferred sequence for each amino acid. What happens when the cell is overloaded by one amino acid codon?<p class="readmore"><br />
<a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">read more...</a></p><br />
</div><br />
<br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Photo Gallery</h3><br />
<div id="photo"><br />
</div><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<br />
<br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Team
Team:Penn State/Team
2012-10-27T03:00:25Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>andreas02 v3.0</title><br />
<br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {clear: both;<br />
color: #fff;<br />
hieght: 200px;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
#brainyBunch {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
background-image: url(https://static.igem.org/mediawiki/2012/0/06/BradyB.jpg);<br />
height: 450px;<br />
width: 600px;<br />
background-repeat: no-repeat;<br />
text-align: right;<br />
}<br />
#brainyBunch h1 {<br />
color: #000;<br />
font-size: 36px;<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-weight: bold;<br />
}<br />
#vicPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 680px;<br />
width: 280px;<br />
color: #FFF;<br />
background-image: url(https://static.igem.org/mediawiki/2012/9/94/Vicfin.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center top;<br />
margin-right: 10px;<br />
padding-right: 10px;<br />
padding-left: 10px;<br />
}<br />
<br />
#kevPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 380px;<br />
width: 280px;<br />
color: #FFF;<br />
background-image: url(https://static.igem.org/mediawiki/2012/c/c4/Alexfin.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center top;<br />
margin-bottom: 10px;<br />
padding-right: 10px;<br />
padding-left: 10px;<br />
}<br />
<br />
#chrisPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 300px;<br />
width: 280px;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/a7/Hanfin.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center bottom;<br />
margin-bottom: 10px;<br />
padding-right: 10px;<br />
padding-left: 10px;<br />
}<br />
<br />
#kaitPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 330px;<br />
width: 280px;<br />
color: #FFF;<br />
background-image: url(https://static.igem.org/mediawiki/2012/6/65/Kevfin.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center top;<br />
margin-right: 10px;<br />
margin-bottom: 10px;<br />
padding-right: 10px;<br />
padding-left: 10px;<br />
}<br />
<br />
#hanPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 400px;<br />
width: 280px;<br />
background-image: url(https://static.igem.org/mediawiki/2012/f/f8/Kaitfin.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center top;<br />
margin-right: 10px;<br />
margin-bottom: 10px;<br />
color: #000000;<br />
padding-right: 10px;<br />
padding-left: 10px;<br />
}<br />
<br />
#alexPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 580px;<br />
width: 280px;<br />
color: #FFF;<br />
background-image: url(https://static.igem.org/mediawiki/2012/c/c6/ChrisB.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center top;<br />
margin-bottom: 10px;<br />
margin-right: 10px;<br />
padding-right: 10px;<br />
padding-left: 10px;<br />
}<br />
<br />
#jyotPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
width: 170px;<br />
color: #666;<br />
background-image: url(https://static.igem.org/mediawiki/2012/5/54/Jyotfin.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center bottom;<br />
margin-right: 15px;<br />
margin-bottom: 10px;<br />
height: 170px;<br />
}<br />
<br />
#thomPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 170px;<br />
width: 150px;<br />
color: #666;<br />
background-image: url(https://static.igem.org/mediawiki/2012/1/13/ThomB.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center bottom;<br />
margin-bottom: 10px;<br />
padding-left: 5px;<br />
}<br />
<br />
#nikPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
float: left;<br />
height: 170px;<br />
width: 150px;<br />
color: #666;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/a4/NikkiPic.jpg);<br />
background-repeat: no-repeat;<br />
background-position: center bottom;<br />
margin-right: 25px;<br />
margin-bottom: 10px;<br />
padding-left: 15px;<br />
}<br />
<br />
#richPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
background-color: #FFF;<br />
float: left;<br />
height: 250px;<br />
width: 200px;<br />
color: #666;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e7/RichardPic.jpg);<br />
background-repeat: no-repeat;<br />
background-position: bottom;<br />
margin-bottom: 10px;<br />
margin-left: 10px;<br />
}<br />
<br />
#salisPic {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
font-size: 14px;<br />
background-color: #FFF;<br />
float: right;<br />
height: 250px;<br />
width: 200px;<br />
color: #666;<br />
background-image: url(https://static.igem.org/mediawiki/2012/1/19/SalisPic.jpg);<br />
background-repeat: no-repeat;<br />
background-position: bottom;<br />
margin-right: 50px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a> </div><br />
<div id="desc"><br />
<h3>We Are...PENN STATE!</h3><br />
<p>The Penn State iGEM team this year consists of 5 undergraduate members and 1 high school student. In the past few months, we have learned the basics of synthetic biology from scratch, had ups and downs, and best of all...HAD FUN! </p><br />
<br />
<br />
<br />
</div><br />
<div id="main"><br />
<br />
<div class="photo" id="brainyBunch"><br />
<br />
</div><br />
<p class="block">Undergraduate Student Researchers </p><br />
<div id="vicPic"><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>Victoria Heasly</p><br />
<p>Victoria Heasley will be a junior next year at Penn State University studying bio-engineering. She currently works as an iGEM team member on the Codon Optimization Project. Other then synthetic biology research, she helps research in the bio mechanics lab during the school year. </p><br />
<p>Outside of school work she is a director of the Engineering Orientation and Networking (EON), which is the orientation through the College of Engineering for all 1,000 first year engineering students. Additionally, she acts as a representative for the Schreyer Honor College to prospective students, families, alumni and guests as a member of the Schreyer Advancement Team. Finally, she is a member of the Presidential Leadership Academy at Penn State. Outside of academic activities, she loves to dance; it has been her passion and favorite hobby for 15 years. She has loved her time at Penn State and can't wait for the next two years. This year on the iGEM team at Penn State, she has the position of &quot;joyologist&quot;.</p><br />
</div><br />
<div id="alexPic"><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>Chris Cetnar</p><br />
<p>Chris is in his third year here at Penn State University focusing my studies in Biology/Vertebrate Physiology. He learned about the field of synthetic biology in a molecular biology class, and knew he had to get involved in this cutting edge research. He joined Penn State's iGEM team this summer and will be heading the promoter directionality project. </p><br />
<p>He is heavily involved with many of Penn State's extracurricular activities like club basketball and recently founded Penn State Dodgeball Club. He holds officer positions in Biology Club, Pre-Dental Society, and Science Lions and he works as a teaching assistant and serve as a resident assistant in the dorm halls. He loves Penn State and wants to wish all the iGEM teams good luck this year! &quot;Imagination is more important than knowledge.&quot; -Einstein</p><br />
</div><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<div id="chrisPic"><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>Hannah Jepsen-Burger</p><br />
<p>Hannah is a junior from Reading, PA majoring in Food Science. She appreciated the applications of synthetic biology in agriculturally-related fields, and hopes to find employment developing gluten substitutes. Hannah is heading the Multiple Start Codon project.</p><br />
</div><br />
<br />
<div id="hanPic"><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>Kait Levin</p><br />
<p>Kait Levin is a Junior in Biological Engineering at Penn State. She joined iGEM in order to understand more about the constructs of life and how they work and is currently working on the Codon Optimization project. Outside of the classroom she is involved with the American Society of Agricultural and Biological Engineers, Women in Engineering Program, Vole (ballet club), and is an Auxiliary Officer with the Penn State Police. She enjoys music festivals, the movie Office Space, and long naps in the sun.</p><br />
</div><br />
<div id="kaitPic"><p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<br />
<p>Kevin Thyne <br />
<p>Kevin is a Junior in Molecular Biology and Biochemistry. He joined iGEM for the opportunity to work in a lab, and do new, exciting research. Kevin is also involved in the American Society of Microbiology (ASM) and the National Society of Collegiate Scholars (NSCS). In his free time he works on chainmail and lamellar armor.<br />
<p></div><br />
<div id="kevPic"><br />
<br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<P>Alexander Woskob</p> <br />
<P>Alexander Woskob is a Junior at State College Area High School. Although Alexander has always wanted to build a space station on the moon, capable of supporting human life, he is instead pursuing his interests in biology and genetics by working on the Penn State iGEM team. Alexander believes science is the agent which will push society to further develop. He looks to better the world through biology and genetics, and maybe one day build a habitat on the moon.</p></div><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<br />
<p class="block">Graduate Student Mentors</p><br />
<div id="jyotPic">Jyotsna Lavanya Pandey</div><br />
<div id="nikPic">Nikki Kapp</div><br />
<div id="thomPic">Thomas McCarthy</div><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p class="block">Instructors </p><br />
<div id="richPic">Dr. Thomas Richard</div><br />
<div id="salisPic">Dr. Howard Salis</div><br />
<p>&nbsp;</p><br />
<h3>&nbsp;</h3><br />
</div><br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="http://andreasviklund.com/"></a></p><br />
</div><br />
</div><br />
<!-- InstanceEndEditable --><br />
</body><br />
<!-- InstanceEnd --></html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State
Team:Penn State
2012-10-27T03:00:02Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<br />
<style type="text/css"><br />
<br />
body {<br />
background:#e0e0e0;<br />
color:#303030;<br />
font:76% tahoma, verdana, sans-serif;<br />
margin:0;<br />
padding:15px 0 0;<br />
}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
margin-top: -49px;<br />
}<br />
<br />
/* Site title */<br />
<br />
#logo {<br />
margin: 15px 0 0<br />
background-image: url(https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg);<br />
width: 150px;<br />
height: 100px;<br />
}<br />
#logo h1 a {<br />
border:none;<br />
color:#505050;<br />
}<br />
#logo h1 a:hover {<br />
border:none;<br />
color:#303030;<br />
text-decoration:none;<br />
}<br />
#logo p {<br />
font-size:1.3em;<br />
color:#505050;<br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {<br />
border-bottom:1px solid #505050;<br />
color:#356C80;<br />
text-decoration:none;<br />
}<br />
a img {<br />
border:0;<br />
}<br />
a:visited {<br />
color: #666;<br />
}<br />
h1 {<br />
font-size:2.2em;<br />
font-weight:400;<br />
margin:0 0 5px;<br />
padding:0;<br />
}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {<br />
font-size:1.4em;<br />
font-weight:400;<br />
margin:0 0 10px;<br />
}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {<br />
margin:0 0 15px 15px;<br />
padding:0;<br />
}<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
<br />
#navitabs {<br />
clear:both;<br />
}<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
border-left:1px solid #fff;<br />
font-size:1.1em;<br />
margin:0 0 4px;<br />
padding:4px 8px;<br />
text-decoration:none;<br />
}<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border-bottom:1px solid #fff;<br />
text-decoration:none;<br />
}<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
color:#fff;<br />
font-size:1.1em;<br />
margin:0 0 5px;<br />
padding:4px 8px 5px;<br />
text-decoration:none;<br />
}<br />
.activenavitab:hover {<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {<br />
border-color:#fff;<br />
color:#fff;<br />
text-decoration:none;<br />
}<br />
<br />
/* Main content */<br />
<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
#main a:hover {<br />
color: #356C80;<br />
}<br />
<br />
/* Sidebar */<br />
<br />
#sidebar {<br />
float:right;<br />
width:150px;<br />
}<br />
#sidebar p {<br />
font-size:0.9em;<br />
line-height:1.3em;<br />
margin:0 0 15px;<br />
}<br />
#sidebar ul {<br />
margin:0 0 15px 0;<br />
padding:0;<br />
}<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
ul.sidelink li {<br />
list-style:none;<br />
margin:0 0 3px;<br />
padding:0;<br />
}<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
display:block;<br />
margin:5px 10px 5px 0;<br />
padding:3px 4px 3px 8px;<br />
text-align:left;<br />
width:140px;<br />
}<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none;<br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
<br />
#footer {<br />
background:#fff;<br />
border-top:1px solid #d8d8d8;<br />
clear:both; margin:0;<br />
padding:0;<br />
}<br />
#footer a {<br />
color:gray;<br />
}<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
#footer p {<br />
color:gray;<br />
font-size:1.1em;<br />
line-height:1.3em;<br />
margin:15px 0 0;<br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
<br />
.photo {<br />
background-color:#f0f0f0;<br />
border:1px solid #d8d8d8;<br />
margin:0 0 15px;<br />
padding:2px;<br />
}<br />
.right {<br />
margin:-8px 0 8px 0;<br />
text-align:right;<br />
}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {<br />
display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Questioning Central Dogma</h3><br />
<p>The Central Dogma of Molecular Biology is the foundation of modern-day synthetic, molecular, and microbiology. While countless innovations in medicine and technology are based on the truth of the doctrine, it does not always accurately predict results in the lab. The Penn State iGEM team questions the central dogma with genetic constructs explore deviations from the norm.</p><br />
<p class="right"><a href="https://2012.igem.org/Team:Penn_State/Project">Projects >></a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Welcome! </h2><br />
<p class="block">Welcome to the Penn State iGEM page! Here you can find information on all of our <a href="https://2012.igem.org/Team:Penn_State/Project" title="projects">projects</a>, <a href="https://2012.igem.org/Team:Penn_State/Notebook" title="Notebook">progress</a>, <a href=https://2012.igem.org/Team:Penn_State/Judging_Criteria>achievements</a> , and much more. </p><br />
<p class="block">We are currently working on three <a href="pages/projects.html" title="projects">projects</a> that question the dogma of the Central Dogma of Biology. The Central Dogma is the commonly held belief that within biology, information flows from the order of nucleotides in DNA to the order of nucleotides in RNA and finally to the order of amino acids in a protein. These proteins then go on to complete a variety of functions that make life work. </p><br />
<p class="block">Molecular biology can be challenging to understand. Check out our Synthetic Biology For Dummies animation below!</p><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/j_3I6E_W1l8" frameborder="0" allowfullscreen></iframe><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Parts
Team:Penn State/Parts
2012-10-27T02:59:09Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {#666;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
tr {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #333;<br />
}<br />
tr a {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #000;<br />
text-decoration: none;<br />
}<br />
tr a:hover {<br />
cursor: pointer;<br />
color: #066;<br />
font-family: Tahoma, Geneva, sans-serif;<br />
text-decoration: none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Parts Submitted to the Registry</h3><br />
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p><br />
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Submitted Parts</h2><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Name</th><br />
<th scope="col">Type</th><br />
<th scope="col">Description</th><br />
<th scope="col">Symbolic Model</th><br />
<th scope="col">Length (bp)</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td><br />
<td>device</td><br />
<td>multiple start codons coding for RFP and sfGFP respectively</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td><br />
<td>device</td><br />
<td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td><br />
<td>device</td><br />
<td>AHL leader with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</br><br />
<br />
<h2>Continuing Research</h2><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<br />
<br />
<tr><br />
<td>theonine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_1</td><br />
<td>complete and sequenced</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_2</td><br />
<td>complete and sequenced</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_3</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_alternating</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<br />
</table><br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters<br />
">Bidirectional Promoters</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23103</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23114</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>complete and sequenced</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
</table><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Parts
Team:Penn State/Parts
2012-10-27T02:58:42Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {#666;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
tr {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #333;<br />
}<br />
tr a {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #000;<br />
text-decoration: none;<br />
}<br />
tr a:hover {<br />
cursor: pointer;<br />
color: #066;<br />
font-family: Tahoma, Geneva, sans-serif;<br />
text-decoration: none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Parts Submitted to the Registry</h3><br />
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p><br />
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Submitted Parts</h2><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Name</th><br />
<th scope="col">Type</th><br />
<th scope="col">Description</th><br />
<th scope="col">Symbolic Model</th><br />
<th scope="col">Length (bp)</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td><br />
<td>device</td><br />
<td>multiple start codons coding for RFP and sfGFP respectively</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td><br />
<td>device</td><br />
<td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td><br />
<td>device</td><br />
<td>AHL leader with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</br><br />
<br />
<h2>Continuing Research</h2><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<br />
<br />
<tr><br />
<td>theonine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_1</td><br />
<td>complete and sequenced</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_2</td><br />
<td>complete and sequenced</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_3</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_alternating</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<br />
</table><br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters<br />
">Bidirectional Promoters</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23103</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23114</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>complete and sequenced</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
</table><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Parts
Team:Penn State/Parts
2012-10-27T02:57:34Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {#666;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
tr {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #333;<br />
}<br />
tr a {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #000;<br />
text-decoration: none;<br />
}<br />
tr a:hover {<br />
cursor: pointer;<br />
color: #066;<br />
font-family: Tahoma, Geneva, sans-serif;<br />
text-decoration: none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Parts Submitted to the Registry</h3><br />
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p><br />
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Submitted Parts</h2><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Name</th><br />
<th scope="col">Type</th><br />
<th scope="col">Description</th><br />
<th scope="col">Symbolic Model</th><br />
<th scope="col">Length (bp)</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td><br />
<td>device</td><br />
<td>multiple start codons coding for RFP and sfGFP respectively</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td><br />
<td>device</td><br />
<td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td><br />
<td>device</td><br />
<td>AHL leader with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</br><br />
<br />
<h2>Continuing Research</h2><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Construct Sequenced</th><br />
<br />
<tr><br />
<td>theonine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_1</td><br />
<td>complete and sequenced</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_2</td><br />
<td>complete and sequenced</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_3</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_alternating</td><br />
<td>complete</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<br />
</table><br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters<br />
">Bidirectional Promoters</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23103</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23114</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>complete and sequenced</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>oligos annealed</td><br />
<br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<br />
</tr><br />
</table><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Parts
Team:Penn State/Parts
2012-10-27T02:51:46Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {#666;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
tr {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #333;<br />
}<br />
tr a {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #000;<br />
text-decoration: none;<br />
}<br />
tr a:hover {<br />
cursor: pointer;<br />
color: #066;<br />
font-family: Tahoma, Geneva, sans-serif;<br />
text-decoration: none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Parts Submitted to the Registry</h3><br />
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p><br />
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Submitted Parts</h2><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Name</th><br />
<th scope="col">Type</th><br />
<th scope="col">Description</th><br />
<th scope="col">Symbolic Model</th><br />
<th scope="col">Length (bp)</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td><br />
<td>device</td><br />
<td>multiple start codons coding for RFP and sfGFP respectively</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td><br />
<td>device</td><br />
<td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td><br />
<td>device</td><br />
<td>AHL leader with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</br><br />
<br />
<h2>Continuing Research</h2><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Construct Sequenced</th><br />
<br />
<tr><br />
<td>theonine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_1</td><br />
<td>complete</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_2</td><br />
<td>complete</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_3</td><br />
<td>complete</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_alternating</td><br />
<td>complete</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<br />
</table><br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters<br />
">Bidirectional Promoters</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td><br />
<td>complete and added to Registry</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23103</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>testing promoter BBa_J23114</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Parts
Team:Penn State/Parts
2012-10-27T02:46:24Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {#666;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
tr {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #333;<br />
}<br />
tr a {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #000;<br />
text-decoration: none;<br />
}<br />
tr a:hover {<br />
cursor: pointer;<br />
color: #066;<br />
font-family: Tahoma, Geneva, sans-serif;<br />
text-decoration: none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Parts Submitted to the Registry</h3><br />
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p><br />
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Submitted Parts</h2><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Name</th><br />
<th scope="col">Type</th><br />
<th scope="col">Description</th><br />
<th scope="col">Symbolic Model</th><br />
<th scope="col">Length (bp)</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td><br />
<td>device</td><br />
<td>multiple start codons coding for RFP and sfGFP respectively</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td><br />
<td>device</td><br />
<td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td><br />
<td>device</td><br />
<td>AHL leader with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</br><br />
<br />
<h2>Continuing Research</h2><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Construct Sequenced</th><br />
<br />
<tr><br />
<td>theonine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_1</td><br />
<td>complete</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_2</td><br />
<td>complete</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_3</td><br />
<td>complete</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_alternating</td><br />
<td>complete</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<br />
</table><br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters<br />
">Bidirectional Promoters</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td>BD1</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BD2</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BD3</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BBa_J23102</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BBa_J23103</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>BBa_J23114</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Parts
Team:Penn State/Parts
2012-10-27T02:42:59Z
<p>H.jepsenburger: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"><br />
<head><br />
<meta http-equiv="content-type" content="text/html; charset=utf-8" /><br />
<meta name="description" content="your description goes here" /><br />
<meta name="keywords" content="your,keywords,goes,here" /><br />
<meta name="author" content="Your Name" /><br />
<title>Index</title><br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
a:visited {#666;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
<br />
<br />
/* Toptabs menu */<br />
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}<br />
#toptabs p {color:gray; text-align:right;}<br />
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}<br />
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}<br />
.activetoptab {<br />
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;<br />
border-bottom: 1px solid #fff;<br />
margin: 0 0 0 4px;<br />
padding: 4px 5px 3px;<br />
text-decoration: none;<br />
color: #282C2D;<br />
}<br />
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-color: black;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
text-decoration: none;<br />
padding-bottom: 10px;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 620px;<br />
margin-top: 0;<br />
margin-right: 5;<br />
margin-bottom: 0;<br />
margin-left: 5;<br />
padding-top: 0;<br />
padding-right: 15px;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
tr {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #333;<br />
}<br />
tr a {<br />
font-family: Tahoma, Geneva, sans-serif;<br />
color: #000;<br />
text-decoration: none;<br />
}<br />
tr a:hover {<br />
cursor: pointer;<br />
color: #066;<br />
font-family: Tahoma, Geneva, sans-serif;<br />
text-decoration: none;<br />
}<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span><br />
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Parts Submitted to the Registry</h3><br />
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p><br />
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Submitted Parts</h2><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Name</th><br />
<th scope="col">Type</th><br />
<th scope="col">Description</th><br />
<th scope="col">Symbolic Model</th><br />
<th scope="col">Length (bp)</th><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td><br />
<td>device</td><br />
<td>multiple start codons coding for RFP and sfGFP respectively</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td><br />
<td>device</td><br />
<td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td><br />
<td>device</td><br />
<td>AHL leader with mCherry and GFP reporters</td><br />
<td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933003">BBa_K933002</a></td><br />
<td>device</td><br />
<td>promoter sequence with upstream RFP and downstream GFP reporters measuring promoter directionality</td><br />
<td><a href="#">design</a></td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</br><br />
<br />
<h2>Continuing Research</h2><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3><br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Construct Sequenced</th><br />
<br />
<tr><br />
<td>theonine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 9-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_1</td><br />
<td>complete</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_2</td><br />
<td>complete</td><br />
<td>yes</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_3</td><br />
<td>complete</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>theonine 6-repeat codon_alternating</td><br />
<td>complete</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>alanine 6-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_1</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_2</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_3</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<tr><br />
<td>threonine 3-repeat codon_alternating</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
<br />
</tr><br />
<br />
</table><br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters<br />
">Bidirectional Promoters</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td>BD1</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BD2</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BD3</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BBa_J23102</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>BBa_J23103</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>BBa_J23114</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<br />
<br/><br />
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3><br />
<br />
<table width="600" border="1"><br />
<tr><br />
<th scope="col">Sequence Name</th><br />
<th scope="col">Status</th><br />
<th scope="col">Part Sequenced</th><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong small length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>complete</td><br />
<td>yes</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2weak med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1weak_RBS2strong med length</td><br />
<td>oligos annealed</td><br />
<td>&nbsp;</td><br />
</tr><br />
<tr><br />
<td>RBS1strong_RBS2strong med length</td><br />
<td>order oligos</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</div><br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:Threonine6_results.png
File:Threonine6 results.png
2012-10-26T23:24:32Z
<p>H.jepsenburger: uploaded a new version of &quot;File:Threonine6 results.png&quot;</p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Codon_Optimization_Results
Team:Penn State/Codon Optimization Results
2012-10-26T23:23:45Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color:#666;}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration: none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration: none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Codon Optimization</h3></br><br />
<p>The genetic code is a degenerate one; there are more 3-part combinations of nucleotides than there are amino acids. The topic of codon optimization-that is, the cell's preference for one codon sequence over another in translation-has been heavily researched in an effort to determine the optimal genetic sequences for an organism. This project looks at the effects of repeated amino acid sequences of varying lengths and codons and their effect on the cell. </p> <br />
</div><br />
<br />
<div id="main"><br />
<h2>Codon Optimization</h2><br />
<iframe width="420" height="315" src="http://www.youtube.com/embed/uspXR2UzP0U" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Codon_Optimization_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Data Collection</h3><br />
<p>A 6-repeated sequence of a single threonine codon was successfully ligated into the initial construct. The resulting fluorescence was measured using a TECAN machine. The results are displayed graphically below.</p><br />
<P><img src="https://static.igem.org/mediawiki/2012/c/cc/Threonine6_results.png"></p><br />
<br />
<P>The figure above demonstrates the fluorescence of both mCherry and GFP when the ribosome translates four 6-repeat sequences of a single codon of threonine. mCherry levels fluctuate while GFP fluorescence remains the same. Further characterization with repeating sequences will provide more quantitative data, but preliminary testing suggests that with constant levels of GFP, the cell is able to send tRNA strands complementary to each threonine codon without disturbing GFP production. Thus, the codon sequence with the highest mCherry expression indicates the most preferred threonine codon.</p> <br />
<br />
<h3>Additional Testing</h3><br />
<p>Several more repeated sequences of identical and non-identical single amino acid codons are intended for testing in this construct. To view the <a href="https://2012.igem.org/Team:Penn_State/Parts">completed and prospective sequences</a>, please visit our <a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a> page<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/MSC_Overview">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:Threonine6_results.png
File:Threonine6 results.png
2012-10-26T23:21:30Z
<p>H.jepsenburger: </p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results
Team:Penn State/Bidirectional Promoters Results
2012-10-26T23:20:17Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<br />
<title>Bidirectional Promoters Overview</title><br />
<br />
<style type="text/css"><br />
body {<br />
background:#e0e0e0;<br />
color:#303030; font:76% tahoma, verdana, sans-serif; <br />
margin:0; <br />
padding:15px 0 0;<br />
}<br />
<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
<br />
#logo h1 a {<br />
border:none; color:#505050;<br />
}<br />
<br />
#logo h1 a:hover {<br />
border:none; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
<br />
#logo p {<br />
font-size:1.3em; <br />
color:#505050; <br />
margin:0 0 20px 0;<br />
}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
<br />
a:hover {<br />
border-bottom:1px solid #505050; <br />
color:#303030; <br />
text-decoration:none;<br />
}<br />
a:visited {color: #666}<br />
<br />
a img {<br />
border:0;<br />
}<br />
<br />
h1 {<br />
font-size:2.2em; <br />
font-weight:400;<br />
margin:0 0 5px; <br />
padding:0;<br />
}<br />
<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
<br />
h3 {<br />
font-size:1.4em; <br />
font-weight:400; <br />
margin:0 0 10px;<br />
}<br />
<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
<br />
ul,ol {<br />
margin:0 0 15px 15px; <br />
padding:0;<br />
}<br />
<br />
li {<br />
margin:0 0 3px 0;<br />
}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {<br />
clear:both;<br />
}<br />
<br />
.navitab {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
border-left:1px solid #fff; <br />
font-size:1.1em; <br />
margin:0 0 4px; <br />
padding:4px 8px; <br />
text-decoration:none;<br />
}<br />
<br />
.navitab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab {<br />
background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#fff; <br />
font-size:1.1em; <br />
margin:0 0 5px; padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activenavitab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
<br />
#desc a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {<br />
float:right; <br />
width:150px;<br />
}<br />
<br />
#sidebar p {<br />
font-size:0.9em; <br />
line-height:1.3em; <br />
margin:0 0 15px;<br />
}<br />
<br />
#sidebar ul {<br />
margin:0 0 15px 0; <br />
padding:0;<br />
}<br />
<br />
#sidebar li {<br />
list-style:none;<br />
}<br />
<br />
ul.sidelink li {<br />
list-style:none; <br />
margin:0 0 3px; <br />
padding:0;<br />
}<br />
<br />
ul.sidelink li a{<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border:none; display:block; <br />
margin:5px 10px 5px 0; <br />
padding:3px 4px 3px 8px; <br />
text-align:left; <br />
width:140px;<br />
text-decoration;none;<br />
}<br />
<br />
ul.sidelink li a:hover {<br />
background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat;<br />
border:none; <br />
text-decoration:none;<br />
}<br />
<br />
/* Footer */<br />
#footer {<br />
background:#fff; <br />
border-top:1px solid #d8d8d8; <br />
clear:both; <br />
margin:0; <br />
padding:0;<br />
}<br />
<br />
#footer a {<br />
color:gray;<br />
}<br />
<br />
#footer a:hover {<br />
color:#303030;<br />
}<br />
<br />
#footer p {<br />
color:gray; <br />
font-size:1.1em; <br />
line-height:1.3em; <br />
margin:15px 0 0; <br />
padding:0;<br />
}<br />
<br />
/* Additional classes */<br />
.photo {<br />
background-color:#f0f0f0; <br />
border:1px solid #d8d8d8; <br />
margin:0 0 15px; <br />
padding:2px;<br />
}<br />
<br />
.right {<br />
margin:-8px 0 8px 0; <br />
text-align:right;<br />
}<br />
<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
<br />
.block a:hover {<br />
color: #333;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
<br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {<br />
clear:both;<br />
margin-top: 15px;<br />
}<br />
<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab {<br />
background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
color:#333; <br />
font-size:1.1em; <br />
margin:0 0 5px; <br />
padding:4px 8px 5px; <br />
text-decoration:none;<br />
}<br />
<br />
.activemaintab:hover {<br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {<br />
border-color:#fff; <br />
color:#fff; <br />
text-decoration:none;<br />
}<br />
ul {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0;<br />
margin: 0;<br />
width: 570px;<br />
color: #333;<br />
list-style-position: inside;<br />
}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Bidirectional Promoters</h3></br><br />
<p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce <br />
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote <br />
and the degree of expression has not been quantified. This project will test the directionality of <br />
several BioBrick promoters to answer these questions. </p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Bidirectional Promoters</h2><br />
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>Palindromic Promoter Circuits</h3><br />
<p><img src="https://static.igem.org/mediawiki/2012/f/fc/BiDirresults.png"></p><br />
<br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<h3>Picture Gallery</h3><br />
<img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /><br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:BiDirresults.png
File:BiDirresults.png
2012-10-26T23:19:48Z
<p>H.jepsenburger: </p>
<hr />
<div></div>
H.jepsenburger
http://2012.igem.org/Team:Penn_State/MSC_Results
Team:Penn State/MSC Results
2012-10-26T23:15:44Z
<p>H.jepsenburger: </p>
<hr />
<div><html><br />
<head><br />
<title>Multiple Start Codons Overview</title><br />
<br />
<style type="text/css"><br />
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}<br />
#container {<br />
background: #fff;<br />
margin: 0 auto 20px;<br />
padding: 5px 20px 20px;<br />
width: 800px;<br />
}<br />
<br />
/* Site title */<br />
#logo {<br />
margin: 15px 0 0;<br />
}<br />
#logo h1 a {border:none; color:#505050;}<br />
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}<br />
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}<br />
<br />
/* Tags */<br />
a {<br />
color: #303030;<br />
text-decoration: none;<br />
border-bottom-width: 1px;<br />
border-top-style: none;<br />
border-right-style: none;<br />
border-bottom-style: none;<br />
border-left-style: none;<br />
border-bottom-color: #505050;<br />
}<br />
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}<br />
a img {border:0;}<br />
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}<br />
h2 {<br />
font-size: 24pt;<br />
font-weight: bolder;<br />
margin: 0 0 12px;<br />
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;<br />
font-style: normal;<br />
color: #356C80;<br />
text-align: center;<br />
}<br />
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}<br />
p {<br />
line-height: 1.5em;<br />
margin: 0 0 15px;<br />
text-align: left;<br />
color: #000;<br />
}<br />
ul,ol {margin:0 0 15px 15px; padding:0;}<br />
li {margin:0 0 3px 0;}<br />
<br />
<br />
/* Navitabs menu */<br />
#navitabs {clear:both;}<br />
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}<br />
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}<br />
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activenavitab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#desc {<br />
clear: both;<br />
color: #fff; /* height:200px; */<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#desc p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 0 0 15px;<br />
width: 325px;<br />
color: #FFF;<br />
}<br />
#desc h3 {<br />
color: #fff;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
text-size: 18pt;<br />
font-size: 16pt;<br />
}<br />
#desc a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
/* Main content */<br />
#main {<br />
border-right: 1px solid #d8d8d8;<br />
float: left;<br />
width: 600px;<br />
margin-top: 0;<br />
margin-bottom: 0;<br />
padding-top: 0;<br />
padding-bottom: 0;<br />
padding-left: 0;<br />
}<br />
<br />
<br />
/* Sidebar */<br />
#sidebar {float:right; width:150px;}<br />
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}<br />
#sidebar ul {margin:0 0 15px 0; padding:0;}<br />
#sidebar li {list-style:none;}<br />
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}<br />
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}<br />
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}<br />
<br />
/* Footer */<br />
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}<br />
#footer a {color:gray;}<br />
#footer a:hover {color:#303030;}<br />
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}<br />
<br />
/* Additional classes */<br />
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}<br />
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}<br />
.right {margin:-8px 0 8px 0; text-align:right;}<br />
.block {<br />
padding: 10px;<br />
background-color: #EBEBEB;<br />
font-size: 12px;<br />
}<br />
.block a: {color: #000;<br />
border: none;<br />
text-decoration:none;<br />
}<br />
.block a:hover {<br />
color: #090;<br />
border: none;<br />
cursor: pointer;<br />
text-decoration:none<br />
} <br />
.hide {display:none;<br />
color: #000;<br />
}<br />
<br />
#blenderAni {<br />
height: 250px;<br />
width: 400px;<br />
background-color: #999;<br />
margin-left: 135px;<br />
}<br />
<br />
<br />
<br />
/*Tabbed Panels*/<br />
#maintabs {clear:both; margin-top: 15px;}<br />
.maintab {<br />
border-bottom: 1px solid #fff;<br />
border-left: 1px solid #fff;<br />
font-size: 1.1em;<br />
margin: 0 0 4px;<br />
padding: 4px 8px;<br />
text-decoration: none;<br />
background-color: #CCCCCC;<br />
background-image: url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif);<br />
background-repeat: no-repeat;<br />
background-position: right top;<br />
}<br />
.maintab:hover {<br />
background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; <br />
border-bottom:1px solid #fff; <br />
text-decoration:none;<br />
}<br />
.activemaintab {background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#333; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}<br />
.activemaintab:hover {color:#fff; text-decoration:none;}<br />
<br />
/* Header description area */<br />
#tabfiller {<br />
clear: both;<br />
color: #fff;<br />
margin: 5px 0 15px;<br />
padding: 0 0 5px 0;<br />
background-image: url(../images/indexNav1.jpg);<br />
background-color: #f0f0f0;<br />
background-repeat: no-repeat;<br />
background-position: left bottom;<br />
text-align: left;<br />
font-size: 18pt;<br />
}<br />
#tabfiller p {<br />
font-size: 10pt;<br />
line-height: 1.3em;<br />
padding: 0 15px 15px 15px;<br />
width: 570px;<br />
color: #333;<br />
}<br />
#tabfiller h2 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 18pt;<br />
<br />
}<br />
#tabfiller h3 {<br />
color: #333;<br />
padding: 15px 15px 0;<br />
text-align: left;<br />
font-size: 14pt;<br />
<br />
#tabfiller a {border-color:#fff; color:#fff; text-decoration:none;}<br />
<br />
#logoiGEM {<br />
height: 56px;<br />
width: 140px;<br />
float: right;<br />
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);<br />
margin-bottom: 15px;<br />
background-repeat: no-repeat;<br />
}<br />
#psuLogo {<br />
height: 65px;<br />
width: 116;<br />
float: right;<br />
padding-top: 11px;<br />
}<br />
a:visited {color: #666}<br />
</style><br />
</head><br />
<br />
<br />
<body><br />
<div id="container"><br />
<div id="logoiGEM"></div><br />
<div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div><br />
<div id="navitabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><br />
<span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><br />
<span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a><br />
</div><br />
<br />
<div id="desc"><br />
<h3>Multiple Start Codons</h3></br><br />
<p>A frustrating yet commonly observed phenomenon in the lab is the production of unexpected proteins. These occurrences may be explainable by multiple start codons in the mRNA strand. Codon slippage is a theory practically untouched by research, and this project aspires to shed some light on the issue.</p><br />
</div><br />
<br />
<div id="main"><br />
<h2>Multiple Start Codons</h2><br />
<div id="prezi"><iframe width="560" height="315" src="http://www.youtube.com/embed/WTr8n0TCu6s?rel=0" frameborder="0" allowfullscreen></iframe></div><br />
<div id="maintabs"><br />
<h2 class="hide">Sample navigation menu:</h2><br />
<a class="maintab" href="https://2012.igem.org/Team:Penn_State/MultipleStartCodons">Overview</a><br />
<span class="hide"> | </span> <a class="maintab" href="https://2012.igem.org/Team:Penn_State/MSC_Design">Design</a><br />
<span class="hide"> | </span> <a class="activemaintab" href="https://2012.igem.org/Team:Penn_State/MSC_Results">Results</a><br />
</div><br />
<br />
<div id="tabfiller"><br />
<h3>TECAN Data </h3><br />
<p>The initial circuit for this project has recently been completed. The relative fluorescence of both RFP and sfGFP reporters was tested in a TECANM1000.</p><br />
<img src="https://static.igem.org/mediawiki/2012/2/24/MSC_results.png"><br />
</p><br />
</div><br />
</div><br />
<br />
<br />
<div id="sidebar"><br />
<h3>Check these Out!</h3><br />
<ul class="sidelink"><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li><br />
</ul><br />
<br />
<h3>Projects</h3><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li><br />
<li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li><br />
</ul><br />
</div><br />
<div id="footer"><br />
<p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>
H.jepsenburger
http://2012.igem.org/File:MSC_results.png
File:MSC results.png
2012-10-26T23:12:49Z
<p>H.jepsenburger: </p>
<hr />
<div></div>
H.jepsenburger