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2024-03-28T17:41:16Z
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http://2012.igem.org/Team:NYMU-Taipei/ymit1
Team:NYMU-Taipei/ymit1
2013-05-08T13:23:13Z
<p>Jayms: Undo revision 300513 by Bert40367 (talk)</p>
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<a <br />
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<a <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymihpa.html">Human Practice</a> · <br />
<a <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymijf.html">Extras</a> · <br />
<a <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymit1">Team</a> · <br />
<a href="https://2012.igem.org/Main_Page">iGEM</a><br />
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<div class="title">Team</div><br />
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<p><span class="subtitle">Instructor</span></p><br />
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<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/d/dd/Ymit1.gif" alt="" /> </div><br />
Chuan-Hsiung Chang<br /><br />
Associate Professor<br /><br />
Center for Systems and Synthetic Biology<br /><br />
Institute of Biomedical Informatics<br /><br />
National Yang Ming University<br /><br />
NYMU Synthetic Biology iGEM Team</p><br />
<p><br /><br />
<br /><br />
</p><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/e/ed/Ymit3.gif" alt="" /></div><br />
<p>Chih-Hsien Yang<br /><br />
Ph.D Candidate, Genome Engineering Laboratory, Institute of Biomedical <br /><br />
Informatics, National Yang Ming University, Taiwan</p><br />
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<div id="team_right"><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/b/b2/Ymit2.gif" alt="" /> </div><br />
Pei-Hong Chen<br /><br />
Postdoctoral Fellow<br /><br />
Institute of Bioinformatics &amp; Center for Systems and Synthetic Biology<br /><br />
National Yang-Ming University, Taiwan</p><br />
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<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/a/ab/Ymit4.gif" alt="" /></div><br />
<p>Jesse Wu is a ctrl+s trigger happy person who loves programming.<br /><br />
He likes listening to cantonese pop and Gershwin's Rhapsody in Blue. <br /><br />
In his spare time, he sings. <br /><br />
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Member</span></p><br />
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<div id="team_left"><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/f/f8/T5.gif" alt="" /></div><br />
I am team leader Shi Hao.<br /><br />
My dream is to let the world see Taiwan. <br /><br />
My job in iGEM is to coordinate everything, and make sure that everyone and everything go well.<br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/1/17/T7.gif" alt="" width="89" height="125" /></div><br />
<p>I am Chun-Kang.<br /><br />
I am responsible for<br />
contacting entrepreneur iGEM team, implementing Chung Hwa Pulp Corporation project, conducting SRB experiment and propagating iGEM to new NYMU students.</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/f/f4/T9.gif" alt="" width="86" height="120" /></div><br />
<p>En-Jie Shih is a second-year student majoring medicine at NYMU. <br /><br />
She is the person in charge of human practice and also helps doing the experiment. </p><br />
<p>&nbsp;</p><br />
<p><br /><br />
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</p><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/2012/4/4b/Ymit11.gif" alt="" width="103" height="132" /></div><br />
<p>Yi Lun is a third-year student specialized in medicine.<br /><br />
He loves movies and soccer.<br />
<br /><br />
He is responsible for Nap gene cloning, modeling and website design.</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/1/19/T14.gif" alt="" width="108" height="138" /></div><br />
<p>I&rsquo;m Chris Yen-Chen Lo. My major is Medical technology &amp; Radiology. <br /><br />
I am responsible for sulfur Oxide Terminator Design, gene construction, experiment performance, universal human practice and oral presentation.<br /><br />
</p><br />
</div><br />
<div id="team_right"><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/5/57/T6.gif" alt="" width="97" height="135" /></div><br />
Ting Yi is in the department of Medicine. <br /><br />
He is responsible for most of the cloning experiment and the primary testing design for part BBa-K896000.<br /><br />
As for human practice, he is responsible for the Taiwan iGEMer's fest.<br />
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<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/0/02/T8.gif" alt="" width="91" height="121" /></div><br />
<p>Chiu Hsun-I is a senior in National Yang Ming University majoring in medicine. <br /><br />
Chiu helped with cell culturing, symbiosis experiment and cloning of SQR gene.<br /><br />
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</p><br />
<p>&nbsp;</p><br />
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<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/e/ec/T10.gif" alt="" width="92" height="123" /></div><br />
<p>My name is Chen Shao-Lun. I am in the Department of Medicine. I&rsquo;m responsible for the Cd2+ cleanup subject in our iGEM team. I designed the circuit and did the wet-lab experiment. </p><br />
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</p><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/b/bb/T12.gif" alt="" width="89" height="120" /></div><br />
<p> Adonis is in medicine department. <br /><br />
Adonis&rsquo; main contribution to NYMU iGEM team includes the design of denitrification, the experiment of Sulfide-quinone Reductase and several human practice. </p><br />
<p>&nbsp;</p><br />
<p><br /><br /><br />
</p><br />
<div class="imgbox" style="margin-left:10px;margin-right:10px;float:left;"><img src="https://static.igem.org/mediawiki/igem.org/5/5b/T15.gif" alt="" width="104" height="134" /></div><br />
<p>Hello, my name is Chung-Yu Huang. I major in Biomedical engineering. <br /><br />
In NYMU-Taipei team, I devoted myself to plan on 2012 Taiwan iGEMer Fest. Also, I participated in many activities which are belong to Human practice part. <br /><br />
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Jayms
http://2012.igem.org/Team:NYMU-Taipei
Team:NYMU-Taipei
2012-10-27T03:17:37Z
<p>Jayms: </p>
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<a <br />
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href="https://2012.igem.org/Team:NYMU-Taipei/ymihpa.html">Human Practice</a> · <br />
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href="https://2012.igem.org/Team:NYMU-Taipei/ymijf.html">Extras</a> · <br />
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href="https://2012.igem.org/Team:NYMU-Taipei/ymit1">Team</a> · <br />
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<p><br />
<br />
We planned to cultivate a special strain of genetically engineered cyanobacteria. With reductases metabolizing nitrogen, sulfur and carbon oxides, our organisms reduce three major pollutants in the modern day. Furthermore, we also focus on the removal of cadmium ions from soil.Combining our engineered cyanobacteria and the concept of endosymbiosis, we grant eukaryotes, ultimately human being, the ability to colonize Venus and expand our territory. <br /><br />
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<div class="section_box" align="justify"><br />
<div class="subtitle"><strong>Welcome to our Campus</strong></div><br />
Founded in 1974, National Yang-Ming University, formerly known as National Yang-Ming College of Medicine, has been adhering to the school motto of "benevolent mind and art, putting knowledge into practice" while training excellent humane doctors to provide service by solving medical problems in remote and rural areas.<br /><br />
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Jayms
http://2012.igem.org/Team:NYMU-Taipei
Team:NYMU-Taipei
2012-10-27T03:15:44Z
<p>Jayms: </p>
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href="https://2012.igem.org/Team:NYMU-Taipei/ymit1">Team</a> · <br />
<a href="https://2012.igem.org/Main_Page">iGEM</a><br />
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<br />
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<br />
<br />
<p>&nbsp;</p><br />
<br />
<p><br />
<br />
We planned to cultivate a special strain of genetically engineered cyanobacteria. With reductases metabolizing nitrogen, sulfur and carbon oxides, our organisms reduce three major pollutants in the modern day. Furthermore, we also focus on the removal of cadmium ions from soil.Combining our engineered cyanobacteria and the concept of endosymbiosis, we grant eukaryotes, ultimately human being, the ability to colonize Venus and expand our territory. <br /><br />
<br /><br />
</p><br />
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<li></li><br />
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<li></li><br />
</ul><br />
<div class="section_box" align="justify"><br />
<div class="subtitle"><strong>Welcome to our Campus !</strong></div><br />
Founded in 1974, National Yang-Ming University, formerly known as National Yang-Ming College of Medicine, has been adhering to the school motto of "benevolent mind and art, putting knowledge into practice" while training excellent humane doctors to provide service by solving medical problems in remote and rural areas.<br /><br />
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Jayms
http://2012.igem.org/Team:NYMU-Taipei
Team:NYMU-Taipei
2012-10-27T03:15:12Z
<p>Jayms: </p>
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<p>Welcome to our Campus !</p><br />
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<p><br />
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We planned to cultivate a special strain of genetically engineered cyanobacteria. With reductases metabolizing nitrogen, sulfur and carbon oxides, our organisms reduce three major pollutants in the modern day. Furthermore, we also focus on the removal of cadmium ions from soil.Combining our engineered cyanobacteria and the concept of endosymbiosis, we grant eukaryotes, ultimately human being, the ability to colonize Venus and expand our territory. <br /><br />
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<div class="subtitle"><strong>Welcome to our Campus !</strong></div><br />
Founded in 1974, National Yang-Ming University, formerly known as National Yang-Ming College of Medicine, has been adhering to the school motto of "benevolent mind and art, putting knowledge into practice" while training excellent humane doctors to provide service by solving medical problems in remote and rural areas.<br /><br />
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Jayms
http://2012.igem.org/Team:NYMU-Taipei
Team:NYMU-Taipei
2012-10-27T03:13:32Z
<p>Jayms: </p>
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<p>Founded in 1974, National Yang-Ming University, formerly known as National Yang-Ming College of Medicine, has been adhering to the school motto of &quot;benevolent mind and art, putting knowledge into practice&quot; while training excellent humane doctors to provide service by solving medical problems in remote and rural areas. </p><br />
<p> Our success is no coincidence but an attribute of excellent allocation of limited resources and the devoted efforts of both the faculty and students, all of which were needed to achieve &quot;brilliant results in teaching and research&quot; within such a short period of time. Due to our research atmosphere, academic accomplishments, innovative knowledge and cultivation of talent, we have well demonstrated our standing as a top university. <br /><br />
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<div class="subtitle"><strong>Welcome to our Campus !</strong></div><br />
Founded in 1974, National Yang-Ming University, formerly known as National Yang-Ming College of Medicine, has been adhering to the school motto of "benevolent mind and art, putting knowledge into practice" while training excellent humane doctors to provide service by solving medical problems in remote and rural areas.<br />
Our success is no coincidence but an attribute of excellent allocation of limited resources and the devoted efforts of both the faculty and students, all of which were needed to achieve "brilliant results in teaching and research" within such a short period of time. Due to our research atmosphere, academic accomplishments, innovative knowledge and cultivation of talent, we have well demonstrated our standing as a top university. <br />
<br />
<br />
<br />
<br />
We planned to cultivate a special strain of genetically engineered cyanobacteria. With reductases metabolizing nitrogen, sulfur and carbon oxides, our organisms reduce three major pollutants in the modern day. Furthermore, we also focus on the removal of cadmium ions from soil.Combining our engineered cyanobacteria and the concept of endosymbiosis, we grant eukaryotes, ultimately human being, the ability to colonize Venus and expand our territory. <br /><br />
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Jayms
http://2012.igem.org/Team:NYMU-Taipei
Team:NYMU-Taipei
2012-10-27T03:13:11Z
<p>Jayms: </p>
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<br />
<br />
<p>&nbsp;</p><br />
<p>Founded in 1974, National Yang-Ming University, formerly known as National Yang-Ming College of Medicine, has been adhering to the school motto of &quot;benevolent mind and art, putting knowledge into practice&quot; while training excellent humane doctors to provide service by solving medical problems in remote and rural areas. </p><br />
<p> Our success is no coincidence but an attribute of excellent allocation of limited resources and the devoted efforts of both the faculty and students, all of which were needed to achieve &quot;brilliant results in teaching and research&quot; within such a short period of time. Due to our research atmosphere, academic accomplishments, innovative knowledge and cultivation of talent, we have well demonstrated our standing as a top university. <br /><br />
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<div class="subtitle"><strong>Welcome to our Campus !</strong></div><br />
We planned to cultivate a special strain of genetically engineered cyanobacteria. With reductases metabolizing nitrogen, sulfur and carbon oxides, our organisms reduce three major pollutants in the modern day. Furthermore, we also focus on the removal of cadmium ions from soil.Combining our engineered cyanobacteria and the concept of endosymbiosis, we grant eukaryotes, ultimately human being, the ability to colonize Venus and expand our territory. <br /><br />
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Jayms
http://2012.igem.org/Team:NYMU-Taipei/ymiq5.html
Team:NYMU-Taipei/ymiq5.html
2012-10-27T03:12:32Z
<p>Jayms: </p>
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<div class="title">Further Experiments after Asia Jamboree</div><br />
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<div align="left"><br />
<br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/0/0a/Ymiqw1.png" alt="" width="502" height="333" /><br /><br />
<p align="center">Sulfite quinine reductase, SQR use hydrogen sulfide, H2S as electron resource to perform photosynthesis<br /><br />
</p></div><br />
In previous experiment, we’ve demonstrated that Sulfide-Quinone Reductase (sqr) expressed in Synechococcus sp. PCC7942 enhanced the growth of the unicellular cyanobacteria. Nevertheless, most of our observations were based on the difference of optical density between wild type and sqr expressing strain. Though it is quite common to estimate the growth of cyanobacteria by measuring the optical density under certain wavelength, such measurement only provides an approximate data for comparison, and various factors can influence the observation accuracy, With regard to that, we conduct another series of experiments via flow cytometer and plate count method to examine the exact cell concentration. <br />
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<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/f/f2/Ymiqw2.png" alt="" width="398" height="314" /><br /><br />
Cyanobacteria contain different of cytochrome. We can detect them by different wavelength fluorescent light.<br /><br />
( http://www.masa.asn.au/phpBB3/viewtopic.php?t=233828&amp;p=960435 )<br /><br />
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<br /><div align="left"><br />
<p><span class="subtitle">Cyanobacteria Culture </span></p><br />
</div><br />
<p>Both wild type and sqr stain of <em>Synechococcus sp. PCC7942</em> were cultivated in BG-11 medium (Allen M.M. 1968) [1]. The initial cell concentration was adjusted to an OD730 of 0.2. The experiment was initialized after the addition of unicellular blue-green algae, and the six-well plates were placed on a 100 r.p.m. shaker.<br />
<br /><br />
</p><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/c/ce/Ymiqw3.png" alt="" width="434" height="334" /><br /><br />
We culture Synechococcus sp. PCC7942 wildtype and sqr transformed one in BG-11 medium with suitable shaker. <br /><br />
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<br /><div align="left"><br />
<p><span class="subtitle">DCMU blocking assay</span></p><br />
</div><br />
<br />
<br />
<p>Freshly made sodium sulfide containing medium was added into the experiment group. The concentration of sodium sulfide was 2.5 mM, and additional 2.5mM of sodium sulfide was added every 24 hours. 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) [2], a chemical which can block photosystem II was also added into both experiment and control group. A positive control group was established by simply cultivated cyanobacteria in fresh medium. As followed, we analyze by flow cytometry analysis, 730 nm absorption assay and plating of bacteria.<br />
<br /><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/2/23/Ymiqw4.png" alt="" width="558" height="262" /><br /><br />
DCMU specifically block photosynthesis system II<br /><br />
( http://5e.plantphys.net/article.php?ch=7&id=75 )<br /><br />
<br /><br />
<br /><br />
</div><br />
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<p><span class="subtitle">Flow cytometry analysis </span></p><br />
</div><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/2/28/Ymiqw5.png" alt="" width="475" height="361" /><br /><br />
We use flow cytometry with multiple wavelength fluorescent light to find out the differences between two groups. <br /><br />
<br /><br />
</div><br />
<p>The automation of viability analysis method via flow cytometer was reported in previous research [3]. In our experiment, cells were analyzed using a flow cytometer FACSCanto. The chlorophyll fluorescence filter set (excitation: 435/40 nm; beam splitter: 510 nm; emission: 515 nm long-pass) were used [3]. By using the automated counting function of FACSCanto, we examined the accurate number of cell. The green fluorescence of the cell area was constrained at an intensity value of 50 to differentiate between red (viable) and green (non-viable) cells [3]. The percentage of red and green cells and the exact cell concentration was calculated.<br />
</p><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/3/3e/Ymiqw6.png" alt="" width="558" height="385" /><br /><br />
Different fluorescent dyes and its wavelength<br />
(http://www.epitomics.com/images/DyLight%20Spectra%20Chart.jpg )<br />
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<div class="title">Results &amp; Discussion</div><br />
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<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/f/f9/Ymiqw7.png" width="600" height="300" /><br /><br />
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<br /><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/b/ba/Ymiqw8.png" width="600" height="283" /><br /><br />
</div><br />
<br /><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/f/fc/Ymiqw9.png" width="600" height="262" /><br /><br />
</div><br />
<br /><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/d/d5/Ymiqw10.png" width="600" height="266" /><br /><br />
</div><br />
<br /><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/b/b1/Ymiqw11.png" width="600" height="291" /><br /><br />
</div><br />
<br /><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/7/72/Ymiqw12.png" width="600" height="249" /><br /><br />
</div><br />
<br /><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/b/bc/Ymiqw13.png" width="600" height="244" /><br /><br />
</div><br />
<br /><br />
<ol><br />
<li>We kill wildtype cyanobacteria with 4oC environment for 2 weeks</li><br />
<li>We find out that when adding Na2S to our DCMU blocked SQR bacteria, they show more fluorescent light(higher photosynthesis ability, live better)</li><br />
<li>We find out that when bacteria are challenged with DCMU, SQR group can live better than Wldtype group, which means SQR gene help cyanobacteria survive in the adversity(DCMU, Na2S)</li><br />
<li>It&rsquo;s very interesting when we compare data:</li><br />
</ol><br />
<br /><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/3/3a/Ymiqw14.png" alt="" width="422" height="302" /><br /><br />
<p align="left">When DCMU challenge bacteria, SQR transformed croup can produce more fluorescent light(higher photosynthesis ability, live better)</div><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/6/6d/Ymiqw15.png" alt="" width="405" height="299" /><br />
<p align="left">In SQR group, we use DCMU to challenge bacteria. Adding Na2S bacteria produce more fluorescent light. </div><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/b/b9/Ymiqw16.png" alt="" width="382" height="280" /><br />
<p align="left">We survey several papers and hypothesize that the fluorescent comes from cytochrome b6f, which is an important role in the downstream of photosynthesis system II and the electron transport chain. (Erwin J. G. Peterman,Stephan-Olav Wenk et al, Fluorescence and Absorption Spectroscopy of the Weakly Fluorescent Chlorophyll a in Cytochrome b6f of Synechocystis PCC6803, Biophysical Journal Volume 75 July 1998 389–398)<br /><br />
<br /><br />
<br /><br />
</div><br />
<div align="left"><br />
<p><span class="subtitle">730 nm absorption</span></p><br />
</div><br />
<p>Based on the flow cytometry result, we double check our bacteria amount by 730nm absorption:</p><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/9/9f/Ymiqw17.png" alt="" width="559" height="394" /><br /><br />
</div><br />
<ol><br />
<li>When challenge bacteria with DCMU, SQR transformed cyanobacteria live better than wildtype(higher photosynthesis, better adversity resistance)</li><br />
<li>It&rsquo;s very interesting that when add Na2S into two kinds of bacteria, it might produce more difficulty for living(maybe the Na2S concentration is wrong? HS- binds to Fe and generate worse environment for bacteria)<br /><br />
<br /><br />
</li><br />
</ol><br />
<div align="left"><br />
<p><span class="subtitle">Plating of bacteria</span></p><br />
</div><br />
<p>Viable cell concentrations were also determined by spreading 0.1 mL of culturing cyanobacteria on BG-11 agar plates. Colony counts were conducted after these plates were incubated for 7 days at room temperature. The plating was performed in duplicates for every sample.</p><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/6/6c/Ymiqw18.png" width="509" height="557" /><br /><br />
<p align="left" >We culture bacteria into solid plate. After incubation for about 10 days, we will count the colonies and compare each group. <br /><br />
<br /><br />
<br /><br />
</div><br />
<div align="left"><br />
<p><span class="subtitle">CO2 consumption analysis</span><br /><br />
</p><br />
</div><br />
<div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/2/25/Ymiqw19.png" alt="" width="407" height="482" /><br /><br />
<p align="left" >We culture two group bacteria with stable lamina CO2 input and compare the CO2 for each group.<br /><br />
</div><br />
<p>It&rsquo;s very interesting that when adding Na2S to both two bacteria group, we get the similar result. We hypothesize that Na2S might generate adversity to bacteria. Thus, It&rsquo;s very important to analyze CO2 consumption. CO2 concentration and analysis were examined by the infrared CO2 analyzer and FL22 Algal CO2 Package respectively [4]. Cyanobacteria samples were cultured for 24 hours following CO2 analysis. CO2 fixation rates were calculated in terms of CO2 exchange rate as the following equation: </p><br />
<div class=out style='text-align:center'><br />
<p align="center" ><img src="https://static.igem.org/mediawiki/2012/d/dd/Ymiqw20.png" alt="" width="433" height="60" /> </div><br />
<p>The HCO3 concentration in the culture medium was also measured with Carbon Dioxide Enzymatic Assay of BQ Kits (Bio-Quant, USA).</p><br />
<div class=out style='text-align:center'><br />
<p align="center" ><img src="https://static.igem.org/mediawiki/2012/b/bc/Ymiqw21.png" alt="" width="558" height="320" /> <br /><br />
<p align="left"> We will analyze CO2 consumption for SQR grop and wildtype cyanobacteria to find out the activity of them. Finally, We will use DCMU to challenge two groups of bacteria and perform this assay again ( http://www.quora.com/Carbon-Dioxide ) <br /><br />
<br /><br />
<br /><br />
</div><div align="left"><br />
<p><span class="subtitle">References</span><br /><br />
</p><br />
</div><br />
<ol><br />
<li><em>Allen MM (1968) Simple conditions for growth of unicellular blue-green algae. J Phycol 4: 1-3</em></li><br />
<li><em>Adaptation to Hydrogen Sulfide of Oxygenic and Anoxygenic Photosynthesis among Cyanobacteria YEHUDA COHEN,'* BO BARKER J0RGENSEN,2 NIELS PETER REVSBECH,2 AND RICARDO POPLAWSKIl H. Steinitz Marine Biology Laboratory, The Hebrew University of Jerusalem, Eilat 88103, Israel,1 and Institute of Ecology and Genetics, University of Aarhus, Ny Munkegade, DK 8000, Aarhus C, Denmark2 Received 1 July 1985/Accepted 22 October 1985</em></li><br />
<li><em>A simple viability analysis for unicellular cyanobacteria using a new autofluorescence assay, automated microscopy, and ImageJ Katja Schulze1,2*, Diana A López1, Ulrich M Tillich1 and Marcus Frohme1 BMC Biotechnology 2011, 11:118 doi:10.1186/1472-6750-11-118</em></li><br />
<li><em>Enhancing CO2 bio-mitigation by genetic engineering of cyanobacteria† Pei-Hong Chen,a Hsien-Lin Liu,a Yin-Ju Chen,a Yi-Hsiang Cheng,a Wei-Ling Lin,a Chien-Hung Yeha and Chuan-Hsiung Chang*ab Received 16th January 2012, Accepted 7th June 2012 DOI: 10.1039/c2ee21124f<br /><br />
</em> <br /><br />
</li><br />
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<li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li><br />
<li><a title="Experiments" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq3.html">Experiments</a></li><br />
<li><a title="Results & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results &amp; References</a></li><li><a title="Further Experiments after Asia Jamboree" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq5.html">Further Experiments after Asia Jamboree</a></li><br />
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<li><a title="Paper-Based Research" href="https://2012.igem.org/Team:NYMU-Taipei/ymis2.html">Paper-Based Research</a></li><br />
<li><a title="Experiment Design" href="https://2012.igem.org/Team:NYMU-Taipei/ymis3.html">Experiment Design</a></li> <br />
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<li><a title="Practical Application in Industrial Waste Detection" href="https://2012.igem.org/Team:NYMU-Taipei/ymis5.html">Practical Applicatioin in <br /><br />
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<li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymin2.html">Methods</a></li><br />
<li><a title="Results" href="https://2012.igem.org/Team:NYMU-Taipei/ymin3.html">Results</a></li><br />
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<li><a title="Experiment Design" href="https://2012.igem.org/Team:NYMU-Taipei/ymic2.html">Experiment Design</a></li><br />
<li><a title="Methods & Materials" href="https://2012.igem.org/Team:NYMU-Taipei/ymic3.html">Methods & Materials</a></li><br />
<li><a title="Results & Discussion" href="https://2012.igem.org/Team:NYMU-Taipei/ymic4.html">Results & Discussion</a></li><br />
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<li><a title="Overview" href="https://2012.igem.org/Team:NYMU-Taipei/ymivenusian.html">Overview</a></li><br />
<li><a title="Introduction" href="https://2012.igem.org/Team:NYMU-Taipei/ymivenusianintro.html">Introduction</a></li><br />
<li><a title="Methods & Materials" href="https://2012.igem.org/Team:NYMU-Taipei/ymivenusianmm.html">Methods &amp; Materials</a></li><br />
<li><a title="Result & Discussion" href="https://2012.igem.org/Team:NYMU-Taipei/ymivenusianrd.html">Result &amp; Discussion</a></li><br />
<li><a title="Conclusion & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymivenusiancr.html">Conclusion &amp; References</a></li><br />
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Jayms
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2012-10-27T03:12:20Z
<p>Jayms: </p>
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Jayms
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2012-10-27T03:11:59Z
<p>Jayms: </p>
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Jayms
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2012-10-27T03:11:41Z
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Jayms
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2012-10-27T03:11:19Z
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Jayms
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2012-10-27T03:11:00Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw16.png
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2012-10-27T03:10:43Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw15.png
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2012-10-27T03:10:24Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw14.png
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2012-10-27T03:09:56Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw13.png
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2012-10-27T03:09:37Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw12.png
File:Ymiqw12.png
2012-10-27T03:09:17Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw11.png
File:Ymiqw11.png
2012-10-27T03:08:48Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw10.png
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2012-10-27T03:08:17Z
<p>Jayms: </p>
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Jayms
http://2012.igem.org/File:Ymiqw9.png
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2012-10-27T03:07:40Z
<p>Jayms: </p>
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Jayms
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2012-10-27T03:07:18Z
<p>Jayms: </p>
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Jayms