http://2012.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=100&target=Hyder&year=&month=
2012.igem.org - User contributions [en]
2024-03-28T09:26:31Z
From 2012.igem.org
MediaWiki 1.16.0
http://2012.igem.org/Team:Arizona_State/NotebookView
Team:Arizona State/NotebookView
2012-10-27T03:43:25Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<br />
<br />
<html><br />
<body><br />
<h1>Notebook</h1><br />
<hr style="color: #800000; height:3px;" /><br />
<br />
<table><br />
<tr><br />
<td scope="col"><table><br />
<tr><br />
<td scope="col"><table width="170px" cellpadding="2"><br />
<tbody><br />
<tr><br />
<td colspan="7" align="center" bgcolor="grey"><strong>June 2012</strong></td><br />
</tr><br />
<tr><br />
<td><strong>S</strong></td><br />
<td><strong>M</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>W</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>F</strong></td><br />
<td><strong>S</strong></td><br />
</tr><br />
<tr><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td bgcolor="white">1</td><br />
<td bgcolor="white">2</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">3</td><br />
<td bgcolor="white">4</td><br />
<td bgcolor="white">5</td><br />
<td bgcolor="white">6</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_07" rel="nofollow">7</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_08" rel="nofollow">8</a></td><br />
<td bgcolor="white">9</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">10</td><br />
<td bgcolor="white">11</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_12" rel="nofollow">12</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_13" rel="nofollow">13</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_14" rel="nofollow">14</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_15" rel="nofollow">15</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_16" rel="nofollow">16</a></td><br />
</tr><br />
<tr><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_17" rel="nofollow">17</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_18" rel="nofollow">18</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_19" rel="nofollow">19</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_20" rel="nofollow">20</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_21" rel="nofollow">21</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_22" rel="nofollow">22</a></td><br />
<td bgcolor="white">23</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">24</td><br />
<td bgcolor="white">25</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_26" rel="nofollow">26</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_27" rel="nofollow">27</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#June_28" rel="nofollow">28</a></td><br />
<td bgcolor="white">29</td><br />
<td bgcolor="white">30</td><br />
</tr><br />
</tbody><br />
</table></td><br />
</tr><br />
<tr><br />
<td scope="row"><table width="170px" cellpadding="2"><br />
<tbody><br />
<tr><br />
<td colspan="7" align="center" bgcolor="grey"><strong>July 2012</strong></td><br />
</tr><br />
<tr><br />
<td><strong>S</strong></td><br />
<td><strong>M</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>W</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>F</strong></td><br />
<td><strong>S</strong></td><br />
</tr><br />
<tr><br />
<td bgcolor="white">1</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_2" rel="nofollow">2</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_3" rel="nofollow">3</a></td><br />
<td bgcolor="white">4</td><br />
<td bgcolor="white">5</td><br />
<td bgcolor="white">6</td><br />
<td bgcolor="white">7</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">8</td><br />
<td bgcolor="white">9</td><br />
<td bgcolor="white">10</td><br />
<td bgcolor="white">11</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_12" rel="nofollow">12</a></td><br />
<td bgcolor="white">13</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_14" rel="nofollow">14</a></td><br />
</tr><br />
<tr><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_15" rel="nofollow">15</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_16" rel="nofollow">16</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_17" rel="nofollow">17</a></td><br />
<td bgcolor="white">18</td><br />
<td bgcolor="white">19</td><br />
<td bgcolor="white">20</td><br />
<td bgcolor="white">21</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">22</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_23" rel="nofollow">23</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_24" rel="nofollow">24</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_25" rel="nofollow">25</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_26" rel="nofollow">26</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_27" rel="nofollow">27</a></td><br />
<td bgcolor="white">28</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">29</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_30" rel="nofollow">30</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#July_31" rel="nofollow">31</a></td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
</tr><br />
</tbody><br />
</table></td><br />
</tr><br />
<tr><br />
<td scope="row"><table width="170px" cellpadding="2"><br />
<tbody><br />
<tr><br />
<td colspan="7" align="center" bgcolor="grey"><strong>August 2012</strong></td><br />
</tr><br />
<tr><br />
<td><strong>S</strong></td><br />
<td><strong>M</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>W</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>F</strong></td><br />
<td><strong>S</strong></td><br />
</tr><br />
<tr><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td bgcolor="white">1</td><br />
<td bgcolor="white">2</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_3" rel="nofollow">3</a></td><br />
<td bgcolor="white">4</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">5</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_6" rel="nofollow">6</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_7" rel="nofollow">7</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_8" rel="nofollow">8</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_9" rel="nofollow">9</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_10" rel="nofollow">10</a></td><br />
<td bgcolor="white">11</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">12</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_13" rel="nofollow">13</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_14" rel="nofollow">14</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_15" rel="nofollow">15</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_16" rel="nofollow">16</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_17" rel="nofollow">17</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_18" rel="nofollow">18</a></td><br />
</tr><br />
<tr><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_19" rel="nofollow">19</a></td><br />
<td bgcolor="white">20</td><br />
<td bgcolor="white">21</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_22" rel="nofollow">22</a></td><br />
<td bgcolor="white">23</td><br />
<td bgcolor="white">24</td><br />
<td bgcolor="white">25</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">26</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_27" rel="nofollow">27</a></td><br />
<td bgcolor="white">28</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_29" rel="nofollow">29</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#August_30" rel="nofollow">30</a></td><br />
<td bgcolor="white">31</td><br />
<td> </td><br />
</tr><br />
</tbody><br />
</table></td><br />
</tr><br />
<tr><br />
<td scope="row"><table width="170px" cellpadding="2"><br />
<tbody><br />
<tr><br />
<td colspan="7" align="center" bgcolor="grey"><strong>September 2012</strong></td><br />
</tr><br />
<tr><br />
<td><strong>S</strong></td><br />
<td><strong>M</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>W</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>F</strong></td><br />
<td><strong>S</strong></td><br />
</tr><br />
<tr><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td bgcolor="white">1</td><br />
</tr><br />
<tr><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_2" rel="nofollow">2</a></td><br />
<td bgcolor="white">3</td><br />
<td bgcolor="white">4</td><br />
<td bgcolor="white">5</td><br />
<td bgcolor="white">6</td><br />
<td bgcolor="white">7</td><br />
<td bgcolor="white">8</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">9</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_10" rel="nofollow">10</a></td><br />
<td bgcolor="white">11</td><br />
<td bgcolor="white">12</td><br />
<td bgcolor="white">13</td><br />
<td bgcolor="white">14</td><br />
<td bgcolor="white">15</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">16</td><br />
<td bgcolor="white">17</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_18" rel="nofollow">18</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_19" rel="nofollow">19</a></td><br />
<td bgcolor="white">20</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_21" rel="nofollow">21</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_22" rel="nofollow">22</a></td><br />
</tr><br />
<tr><br />
<td bgcolor="white">23</td><br />
<td bgcolor="white">24</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_25" rel="nofollow">25</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_26" rel="nofollow">26</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_27" rel="nofollow">27</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_28" rel="nofollow">28</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_29" rel="nofollow">29</a></td><br />
</tr><br />
<tr><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#September_30" rel="nofollow">30</a></td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
</tr><br />
</tbody><br />
</table></td><br />
</tr><br />
<tr><br />
<td scope="row"><table width="170px" cellpadding="2"><br />
<tbody><br />
<tr><br />
<td colspan="7" align="center" bgcolor="grey"><strong>October 2012</strong></td><br />
</tr><br />
<tr><br />
<td><strong>S</strong></td><br />
<td><strong>M</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>W</strong></td><br />
<td><strong>T</strong></td><br />
<td><strong>F</strong></td><br />
<td><strong>S</strong></td><br />
</tr><br />
<tr><br />
<td> </td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_1" rel="nofollow">1</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_2" rel="nofollow">2</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_3" rel="nofollow">3</a></td><br />
<td bgcolor="white">4</td><br />
<td bgcolor="white">5</td><br />
<td bgcolor="white">6</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">7</td><br />
<td bgcolor="white">8</td><br />
<td bgcolor="white">9</td><br />
<td bgcolor="white">10</td><br />
<td bgcolor="white">11</td><br />
<td bgcolor="white">12</td><br />
<td bgcolor="white">13</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">14</td><br />
<td bgcolor="white">15</td><br />
<td bgcolor="white">16</td><br />
<td bgcolor="white">17</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_18" rel="nofollow">18</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_19" rel="nofollow">19</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_20" rel="nofollow">20</a></td><br />
</tr><br />
<tr><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_21" rel="nofollow">21</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_22" rel="nofollow">22</a></td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_23" rel="nofollow">23</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_24" rel="nofollow">24</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_25" rel="nofollow">25</td><br />
<td bgcolor="white"><a target="NB" href="https://2012.igem.org/Team:Arizona_State/Notebook#October_26" rel="nofollow">26</td><br />
<td bgcolor="white">27</td><br />
</tr><br />
<tr><br />
<td bgcolor="white">28</td><br />
<td bgcolor="white">29</td><br />
<td bgcolor="white">30</td><br />
<td bgcolor="white">31</td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
</tr><br />
</tbody><br />
</table></td><br />
</tr><br />
</table></td><br />
<td scope="col"><iframe width="785" height="925" name="NB" src="https://2012.igem.org/Team:Arizona_State/Notebook"></td><br />
</tr><br />
</table><br />
<br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T04:00:21Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to its high binding affinity to biotin, streptavidin serves as one of the major component of this project. <br />
</p><br />
<p><br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Because of streptavidin's high biotin binding affinity, it will allow our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
</p><br />
<p><br />
<br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
</p><br />
<p><br />
<br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. By inducing topoisomerase/split bgal fusion protein expression, we will be able to generate Chimeric probes <i>in vivo</i> that can be easily His-tag purified and tested. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome in order to test the function of our chimeric probes on cell lysates and mock water samples.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:59:14Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to its high binding affinity to biotin, streptavidin serves as one of the major component of this project. <br />
</p><br />
<p><br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Because of streptavidin's high biotin binding affinity, it will allow our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
</p><br />
<p><br />
<br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
</p><br />
<p><br />
<br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. By inducing topoisomerase/split bgal fusion protein expression, we will be able to generate Chimeric probes ''in vivo'' that can be easily His-tag purified and tested. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome in order to test the function of our chimeric probes on cell lysates and mock water samples.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:58:38Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to its high binding affinity to biotin, streptavidin serves as one of the major component of this project. <br />
</p><br />
<p><br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Because of streptavidin's high biotin binding affinity, it will allow our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
</p><br />
<p><br />
<br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
</p><br />
<p><br />
<br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. By inducing topoisomerase/split bgal fusion protein expression, we will be able to generate Chimeric probes ''in vivo'' that can be easily His-tag purified and tested. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:56:04Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to its high binding affinity to biotin, streptavidin serves as one of the major component of this project. <br />
</p><br />
<p><br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Because of streptavidin's high biotin binding affinity, it will allow our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
</p><br />
<p><br />
<br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
</p><br />
<p><br />
<br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:55:40Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to its high binding affinity to biotin, streptavidin serves as one of the major component of this project. <br />
<br /><br /><br />
<br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Because of streptavidin's high biotin binding affinity, it will allow our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
<br /><br /><br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
<br /><br /><br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:54:30Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to its high binding affinity to biotin, streptavidin serves as one of the major component of this project. <br />
<br /><br /><br />
<br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Because of streptavidin's high biotin binding affinity, it will allow our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
<br /><br /><br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
<br /><br /><br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:53:59Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to the high binding affinity to biotin streptavidin serves as one of the major component of this project. <br />
<br /><br /><br />
<br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Because of streptavidin's high biotin binding affinity, it will allow our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
<br /><br /><br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
<br /><br /><br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:53:09Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to the high binding affinity to biotin streptavidin serves as one of the major component of this project. <br />
<br /><br /><br />
<br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Streptavidin has a high binding affinity to biotin, so it will our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
<br /><br /><br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
<br /><br /><br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:52:58Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to the high binding affinity to biotin streptavidin serves as one of the major component of this project. <br />
<br /><br />
<br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Streptavidin has a high binding affinity to biotin, so it will our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
<br /><br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
<br /><br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:52:20Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to the high binding affinity to biotin streptavidin serves as one of the major component of this project. <br />
<br />
<br />
As a proof of concept for our design, our team designed and assembled fusions of streptavidin (strep) and the split beta-galactosidase (bgal) fragments. Streptavidin has a high binding affinity to biotin, so it will our fusion proteins to easily bind onto the ends of biotinylated DNA fragments.<br />
<br />
The addition of a poly-histidine tag (His-tag) makes it possible to generate the fusion proteins in E. Coli, then isolate and purify them using a nickle binding column. Mixtures of His-purified strep-tagged bgal fragments and single stranded biotinylated DNA will generate DNA-Protein Chimera 'probes' as the streptavidin binds to the biotinylated end of the DNA. <br />
<br />
By creating probes using complementary strands of DNA of varying lengths, we can confirm that DNA-Protein Chimeric probes generated in E. Coli will create a colorimetric response when kept in close proximity. This will allow us to characterize the behavior of split bgal fusion probes as a function of the distance that they are separated - which can be controlled by altering the length of the ssDNA, or by creating two probes that bind to the same template ssDNA at different sites separated by a variable length.<br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:46:28Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to the high binding affinity to biotin streptavidin serves as one of the major component of this project. <br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Chimeric_Reporter
Team:Arizona State/Chimeric Reporter
2012-10-04T03:44:43Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<body><br />
<h1>DNA-Protein Chimera Biosensor</h1><br />
<h2>Overview</h2><br />
<p><br />
There are various biosensors on the market but the state of the art technology is based upon Polymerase Chain Reaction and nanotechnology, which involves gold plated probes and requires specialized skills to use. Despite the extreme accuracy of the device, the affordability, and longer diagnostic time has made the technology scarce in the field. In order to make biosensing technology more accessible to those with few resources and the greatest need, the team worked on generating a cost effective, highly accurate and user-friendly organic biosensor. The components of the sensor will be produced in non-pathogenic E. coli. The sensor is made up of a protein head and DNA tail. The protein head is an enzyme that turns a colorless substrate (X-gal) blue. The enzyme is split in half, so that when the sensor is dissolved in water it cannot produce blue color. When pathogenic target DNA is present, two DNA sensor tails bind the target, the split enzyme assembles, and blue color is produced. Color provides a user-friendly output that is familiar to non-skilled users. <br />
</p><br />
<br />
<h2>Topoisomerase</h2><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2012/8/8f/TopoDiagram.png" width="800" height="500"><br />
</p><br />
<p><br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
</p><br />
<h2>Design Scheme</h2><br />
<p><br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
</p><br />
<h2>Reporter System</h2><br />
<p><br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
</p><br />
<p><br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.<br />
</p><br />
<br />
<h2>Streptavidin</h2><br />
<p><br />
Purified and extracted from the bacteria Streptomyces avidinii, Streptavidin posses a high binding affinity for biotin, with a dissociation constant of 10^-14–10^–16 M ( Laitinen et al.). With such high dissociation constant, the bonding of streptavidin to biotin is considered as one of the strongest non covalent bonding in nature. Due to the high binding affinity to biotin streptavidin serves as one of the major component of this project. <br />
</p></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-04T03:44:30Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:20px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:100px;" /><br />
</a><br />
</td><br />
<td align="center"><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:591px;" /><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="125" /><br />
</td><br />
</table><br />
<br /><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">The Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin">Cell Surface Biosensor</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">DNA-Protein Chimera Biosensor</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">BioBricks</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Accomplishments">Judging Criteria</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Community Outreach</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">University Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Case Studies</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Ethical_Conditions">Ethical Considerations</a> </li> <br />
<br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-04T03:04:38Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:20px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:100px;" /><br />
</a><br />
</td><br />
<td align="center"><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:591px;" /><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="125" /><br />
</td><br />
</table><br />
<br /><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin">Cell Surface Biosensor</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">DNA Biosensor</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA">ssDNA Probe Design</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">BioBricks</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Accomplishments">Judging Criteria</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Community Outreach</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">University Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Case Studies</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Ethical_Conditions">Ethical Considerations</a> </li> <br />
<br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-04T03:02:11Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:20px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:100px;" /><br />
</a><br />
</td><br />
<td align="center"><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:591px;" /><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="125" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin">Cell Surface Biosensor</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">DNA Biosensor</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA">ssDNA Probe Design</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">BioBricks</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Accomplishments">Judging Criteria</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Community Outreach</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">University Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Case Studies</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Ethical_Conditions">Ethical Considerations</a> </li> <br />
<br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-04T02:59:05Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 5px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:100px;" /><br />
</a><br />
</td><br />
<td align="center"><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:591px;" /><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="125" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin">Cell Surface Biosensor</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">DNA Biosensor</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA">ssDNA Probe Design</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">BioBricks</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Accomplishments">Judging Criteria</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Community Outreach</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">University Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Case Studies</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Ethical_Conditions">Ethical Considerations</a> </li> <br />
<br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-04T02:36:23Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12<br />
<br />
Picked the colonies again and started new liquid cultures (5mL LB amp).<br />
<br />
Discarded cultures for 8/7/12<br />
<br />
8/9/12<br />
<br />
Miniprepped 3mL of each 8/8/12 culture and nanodropped:<br />
<br />
gfpt1 - 155 ng/uL<br />
<br />
gfpt2 - 114 ng/uL<br />
<br />
Digested gfpt1 and gfpt2 with X and P<br />
<br />
Ran on a 1% agarose gel with the digested GFP plasmid<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel080912<br />
<br />
Made glyercol stocks with aliquot of the remaining liquid cultures<br />
<br />
<br />
8/13/12<br />
<br />
Prepared sequencing samples <br />
<br />
*Sample w/ Primer*<br />
GFPT1 w/ VF2<br />
GFPT1 w/ VR<br />
GFPT2 w/ VF2<br />
GFPT2 w/ VR<br />
<br />
200ng of DNA + 16 pmol of primer<br />
<br />
Annealed oligos again GFPT1/2<br />
<br />
Repeated ligation of oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Followed Haynes assembly protocol instead of standard DH5alpha protocol. (http://openwetware.org/wiki/Haynes:Assembly101)<br />
<br />
Transformed ligations into competent DH5alpha<br />
<br />
Plated on amp treated plates<br />
<br />
8/14/12<br />
<br />
Took pictures of plates<br />
<br />
Green-white screened plates<br />
<br />
Picked 4 white colonies from each of gfpt1/2 plates <br />
<br />
Made 5mL LB amp cultures of each colony<br />
<br />
Delivered GFPT1/2 dna samples to biodesign for sequencing (samples from 8/13/12)<br />
<br />
8/15/12<br />
<br />
Miniprepped 3mL of each liquid culture of GFPT1/2<br />
<br />
Prepared glycerol stocks using 100uL of each liquid culture<br />
<br />
Nanodropped samples:<br />
<br />
GFPT1-1 - 172.6 ng/uL<br />
GFPT1-2 - 203.7 ng/uL<br />
GFPT1-3 - 197.4 ng/uL<br />
GFPT1-4 - 178.9 ng/uL<br />
GFPT2-1 - 107.3 ng/uL<br />
GFPT2-2 - 131.2 ng/uL<br />
GFPT2-3 - 145.5 ng/uL<br />
GFPT2-4 - 172.0 ng/uL<br />
<br />
8/17/12 <br />
<br />
GFPT1 sequence confirmed<br />
<br />
Prepared aliquots of GFPT2 minipreps from 8/15/12 for sequencing<br />
<br />
Delivered GFPT2 samples to biodesign for sequencing<br />
<br />
8/19/12 <br />
<br />
GFPT2 sequences confirmed<br />
<br />
<br />
8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
8/29/12<br />
<br />
Discarded GFPT1/2 cultures from 8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
Digested GFPT1/2 with X and S<br />
<br />
Ran a 1% agarose gel with GFPT1/2 digestions <br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel082912<br />
<br />
Cut out inserts and GFPT2 backbone and stored in 4C for gel extraction and tandum repeat assembly experiments<br />
<br />
8/30/12<br />
<br />
Prepared extra glyercol stocks of GFPT1/2 cultures from 8/29/12<br />
<br />
Miniprepped 3mL of each culture, stored at -20C<br />
<br />
9/19/12<br />
<br />
Set up VF2/VR endpoint PCR for double transform minipreps<br />
<br />
1-1, 1-1I, 1-2, 1-2I, 1-3, 1-3I, 2-1, 2-1I, 2-2, 2-2I, 2-3, 2-3I, GFPT1 (positive controls), GFPT2 (positive controls)<br />
<br />
<br />
Annealing temp set to 55C for 25 cycles<br />
<br />
<br />
Resuspended GFPT1 probe and GFPT2 probe oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
9/25/12<br />
<br />
Resuspended pSB1A2 FWD and pSB1A2 REV (amp resistance primers) oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
Prepared 1.6uM dilutions (500uL)<br />
<br />
Did endpoint PCR using pSB1A2 primer pair on<br />
<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG, 1-1, 1-1I, 2-1, 2-1I, GFPT1, GFPT2<br />
<br />
Did endpoint PCR using VF2/VR primer pair on<br />
<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG<br />
<br />
Annealing temp 55C for 25 cycles, stored products at -20C<br />
<br />
Made 3mL liquid cultures of the shipping vector (pSB1C3 with RFP insert) in DH5alpha in chloramphenicol resistant LB<br />
<br />
Made 10mL liquid cultures of:<br />
<br />
Topo in kanamycin<br />
<br />
topo D168A in kanamycin<br />
<br />
topo + GFPT1 in kanamycin + ampicillin<br />
<br />
topo + GFPT2 in kanamycin + ampicillin <br />
<br />
stored @ 37C<br />
<br />
9/26/12<br />
<br />
Picked two new colonies for topo + GFPT2 and grew 10mL cultures of amp+kan LB broth for each<br />
<br />
Ran a gel with:<br />
<br />
Hyperladder I, GFPT1 pSB1A2, GFPT2 pSB1A2, Topo pSB1A2, Topo D168A pSB1A2, Topo D168A VF2/VR, Topo VF2/VR, GFPT1 VF2/VR, GFPT2 VF2/VR, Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092612-1 I ALSO UPDATED THE LIST OF STUFF IN THIS GEL (see line above this one)<br />
<br />
Ran a gel with PCR samples from 9/19 and 9/25:<br />
<br />
Hyperladder I, 1-1, 1-1I, 2-1, 2-1I, 2-1I(VF), 2-1(VF), 1-1I(VF), 1-1(VF), Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092612-2<br />
<br />
Samples in wells 2-5 used the pSB1A2 primer pair. Samples in wells 6-9 used VF2/VR primer pair.<br />
<br />
Revived GFPT1/2 from glycerol stocks (from 8/9 and 8/15) in 5mL LB amp each.<br />
<br />
Nanodropped miniprepped DNA samples:<br />
<br />
pSB1C3 I - 253.75 ng/uL<br />
<br />
pSB1C3 II - 258.38 ng/uL<br />
<br />
Topo - 24.84 ng/uL<br />
<br />
Topo I - 17.33 ng/uL<br />
<br />
Topo D168A - 7.24 ng/uL<br />
<br />
Topo D168A I - 15.62 ng/uL<br />
<br />
1-1 - 16.49 ng/uL<br />
<br />
1-1I - 16.46 ng/uL<br />
<br />
2-1 - 142.05 ng/uL<br />
<br />
2-1I - 16.98 ng/uL<br />
<br />
Picked new colonies from Topo, Topo D168A, Topo + G1, Topo + G2 and made 1mL colonies in their respective medias<br />
<br />
Stored at -37C<br />
<br />
9/27/12<br />
<br />
Prepared serial dilutions of GFPT2 plasmid 1:10, 1:100, 1:1000, 1:10000<br />
<br />
Prepared primer mixes for pSB and VF/VR primers <br />
<br />
Prepared realtime PCR plate, ran RT-qPCR with annealing temp 57<br />
<br />
Miniprepped GFPT1/2 cultures from 9/26<br />
<br />
Nanodropped Miniprepped DNA:<br />
<br />
GFPT1 - 276.12 ng/uL<br />
<br />
GFPT2 - 219.84 ng/uL<br />
<br />
Used 100uL of each 1mL culture from 9/26 to seed 10 mL cultures in their respective media<br />
<br />
Added 10uL of 1M IPTG to each culture ~4 hours after seeding<br />
<br />
Removed cells from 37C ~4 hours after IPTG inducing<br />
<br />
Pelleted and lysed following the bugbuster protocol (http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2012/05/22) (used lysonase for <br />
Topo and Topo D168A only)<br />
<br />
HIS purified proteins using the Zymo HIS purification kit<br />
<br />
<br />
Digested pSB1C3 plasmid with X and P<br />
<br />
Sent protein samples (HIS purified samples from 9/19, 20uL) and ssDNA control (GFPT1/2 probe oligos 20uL @ 10uM) for mass spec<br />
<br />
9/28/12<br />
<br />
Resuspended 8 new oligos, final volume 100 uM each<br />
<br />
Annealed pet29 top/bot oligos<br />
<br />
Redid the RT-PCR, 3x primer concentration, added 1:1 plasmid concentration<br />
<br />
Made 1.6uM aliquots of primer stocks 1-7 (including topo add X primer previously ordered)<br />
<br />
Diluted aliquot of Topo D168A plasmid 4:10<br />
<br />
Diluted PSV plasmid 2:20<br />
<br />
Performed Endpoint PCR on: <br />
<br />
Topo D168A<br />
using primers 1,2 and primers 1,3<br />
<br />
PSV using primers 4-5 and primers 6-7<br />
<br />
4 samples with low primer concentration, 4 samples with twice as much primer (labelled 'H')<br />
<br />
Miniprepped Topo1 2, Topo1 3, Topo1 4 (biobricked Topo D168A without T7, multiple colonies from ligation into shipping vector)<br />
<br />
Nanodropped:<br />
<br />
Topo1 2 - 63.63 ng/uL<br />
<br />
Topo1 3 - 75.07 ng/uL<br />
<br />
Topo1 4 - 184.93 ng/uL<br />
<br />
1mL chloramphenicol cultures of GFPT1/GFPT2 in shipping vector prepared<br />
<br />
<br />
Digested Topo1 2, Topo1 3, Topo1 4 with E and P<br />
<br />
Ran digested Topo plasmids on 1% agarose gel with Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092812<br />
<br />
<br />
Revived cultures of 2xGFPT1, 2xGFPT2, Topo, and Topo D168A (4 mL cultures each in their respective medias)<br />
<br />
T5 exonuclease treated miniprepped plasmids (1-1, 1-1I) <br />
<br />
A1 1-1I + t5<br />
<br />
B1 1-1 + t5<br />
<br />
A2 1-1I untreated<br />
<br />
B2 1-1 untreated<br />
<br />
9/29/12<br />
<br />
Miniprepped GFPT1, GFPT2, Topo, and Topo D168A (Topo cultures separated into 3mL and 1mL minipreps)<br />
<br />
Nanodropped miniprepped DNA:<br />
<br />
GFPT1 I - 275.29 ng/uL<br />
<br />
GFPT1 II - 101.08 ng/uL<br />
<br />
GFPT2 I - 222.11 ng/uL<br />
<br />
GFPT2 II - 230.52 ng/uL<br />
<br />
Topo I - 117.97 ng/uL<br />
<br />
Topo II - 70.28 ng/uL<br />
<br />
Topo D168A I - 69.47 ng/uL<br />
<br />
Topo D168A II - 51.87 ng/uL<br />
<br />
HIS purified crude lysates from 9/27/12 (Topo, Topo D168A, Topo + GFPT1, Topo + GFPT2, all IPTG induced)<br />
<br />
Digested Topo I plasmid (pet29a) with E and X <br />
<br />
Ran a gel with Hyperladder I, 1-2 1-3 4-5 and 6-7 PCR products, pet29a digestion, and pSB1C3 digestion<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE "ASUiGEM2012_gel092912 fragment pcr confirmation"<br />
<br />
Confirmed that PCR made amplicons <br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092912-1<br />
<br />
Excised bands for digested pet29a and pSB1C3 plasmids<br />
<br />
Gel extracted 4 gel fragments (2 wells per sample: digested pSB1C3 plasmid, digested pet29a plasmid)<br />
<br />
Nanodropped gel extractions:<br />
<br />
Digested pSB1C3 - 25.54 ng/uL<br />
<br />
Digested pet29a - 22.95 ng/uL<br />
<br />
Set up a bradford assay of topo, topo D168A, topo + G1, topo + G2 (5uL protein, 10uL protein, 20uL protein + 200uL reagent)<br />
<br />
Used ~100uL aliquot of BL21 competent glycerol stock to seed 10mL of LB medium (no antibiotic), stored at 37C<br />
<br />
Ran 1% agarose gel with samples: Hyperladder I, A1, B1, A2, B2 and Hyperladder I alpha, s+l+a 1a,s+l+a 2c,s+l+a (2b)strep samples<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092912 alpha, s+l+a 1a,s+l+a 2c,s+l+a (2b)-2 THE DESCRIPTION OF THIS GEL IS UPDATED TO (SEE ABOVE)<br />
<br />
Did bug buster protocol to lyse BL21 control culture (used lysonase)<br />
<br />
Ligated digested pet29a with pet29 top/bot annealed oligos<br />
<br />
Transformed ligation using invitrogen DH5alpha transformation protocol<br />
<br />
Prepared LB kanamycin plates<br />
<br />
plated transformed ligations on prewarmed kanamycin plates<br />
<br />
9/30/12<br />
<br />
Pet29a plates did not grow<br />
<br />
Kinase treated pet29a oligos<br />
<br />
Annealed kinase treated pet29a oligos<br />
<br />
Ligated digested pet29a (from gel extraction) with kinase treated oligos<br />
<br />
Transformed ligations into DH5alpha, used topo plasmid as a positive control<br />
<br />
Plated transformations on kanamycin plates and stored overnight at 37C<br />
<br />
HIS purified BL21 control crude lysate<br />
<br />
Set up a bradford assay with:<br />
<br />
uninduced & induced topo protein extractions from 9/19<br />
<br />
uninduced & induced topo D168A protein extractions from 9/19<br />
<br />
BL21 control lysate<br />
<br />
Topo, Topo D168A, Topo + G1, Topo + G2 from 9/27 <br />
<br />
All samples prepared (10uL protein, 20uL protein + 200uL reagent)<br />
<br />
Miniprepped GFPT1 1,2,3 and GFPT2 (17,18,26) (~600uL of each) (1mL liquid cultures made from colonies on the chloramphenicol plates of GFPT1 and GFPT2 ligated into the shipping vector)<br />
<br />
Nanodropped:<br />
<br />
GFPT1-1 - 38.5 ng/uL<br />
<br />
GFPT1-2 - 77.6 ng/uL<br />
<br />
GFPT1-3 - 74.8 ng/uL<br />
<br />
GFPT2-17 - 61.6 ng/uL<br />
<br />
GFPT2-18 - 65.9 ng/uL<br />
<br />
GFPT2-26 - 64.2 ng/uL<br />
<br />
Ran a 1% agarose gel with 1-1, 1-2, 1-3, 2-17, 2-18, 2-26 plasmid miniprep samples and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel093012-1<br />
<br />
Prepared a 1:2 dilution of GFPT2 plasmid from 9/27 miniprep <br />
<br />
Treated 5uL of diluted plasmid with 5uL of water, BL21 protein, topo protein, topo D168A protein<br />
<br />
Incubated 30 minutes at 37C<br />
<br />
Ran a 1% agarose gel with protein treated target plasmid samples and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel093012-2<br />
<br />
Digested GFPT2 plasmid with X (let run at 37C for 30 minutes)<br />
<br />
Used DNA clean up kit on digested GFPT2 <br />
<br />
Nanodropped digested GFPT2:<br />
<br />
GFPT2(X) - 39.85 ng/uL<br />
<br />
Prepared DNA seq samples using VF2 and VR<br />
<br />
Sample# - PrimerPair - DNA sample (sample 1, GFPT2 uncut + VF2; sample 2, GFPT2 uncut + VR)<br />
<br />
1/2 - FWD/REV - uncut GFPT2 plasmid<br />
<br />
3/4 - FWD/REV - cut GFPT2 plamid<br />
<br />
5/6 - FWD/REV - 2:1 uncut:cut GFPT2 plasmid mixture<br />
<br />
7/8 - FWD/REV - 1:1 uncut:cut GFPT2 plasmid mixture<br />
<br />
9/10 - FWD/REV - 1:2 uncut:cut GFPT2 plasmid mixture<br />
<br />
11/12 - FWD/REV - 1-2 miniprep sample from 9/19 double transformations<br />
<br />
13/14 - FWD/REV - 1-2I <br />
<br />
15/16 - FWD/REV - 1-3<br />
<br />
17/18 - FWD/REV - 1-3I<br />
<br />
19/20 - FWD/REV - 2-1<br />
<br />
21/22 - FWD/REV - 2-1I<br />
<br />
23/24 - FWD/REV - 2-2<br />
<br />
25/26 - FWD/REV - 2-2I<br />
<br />
27/28 - FWD/REV - 2-3<br />
<br />
29/30 - FWD/REV - 2-3I<br />
<br />
10/2/12<br />
<br />
Prepared PCR tubes with 10uL Topo1 4 (25ng/uL topo d168a in the shipping vector), 10uL GFPT2-26 (25ng/uL GFPT2 in the shipping vector), and 10uL GFPT1-3 (25ng/uL GFPT1 in the shipping vector)<br />
<br />
Labelled the tubes K891234, K891999, K891000 respectively and shipped overnight to iGEM Headquarters<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE BELOW THIS IS NEW<br />
<br />
Topo D168A treated DNA samples, incubated for 10 minutes at 37C:<br />
<br />
Omega fragment PCR amplicon<br />
<br />
Topo coding sequence PCR<br />
<br />
GFPT1 VF2/VR PCR amplicon<br />
<br />
Ran a 1% agarose gel containing untreated omega PCR, untreated topo PCR, untreated GFPT1 PCR, treated omega PCR, treated topo PCR, treated GFPT1 PCR, and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM_gel100212<br />
<br />
10/3/12<br />
<br />
Protein treated GFPT2 plasmid, incubated for 10 minutes at 37C:<br />
<br />
BL21 HIS-purified Control Lysate<br />
<br />
Topo<br />
<br />
Topo D168A<br />
<br />
Ran a 1% agarose gel containing GFPT1, GFPT1 + BL21 protein, GFPT1 + Topo, GFPT1 + Topo D168A, Topo D168A (no DNA), and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE Photo10031712 annotated</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-04T02:35:32Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12<br />
<br />
Picked the colonies again and started new liquid cultures (5mL LB amp).<br />
<br />
Discarded cultures for 8/7/12<br />
<br />
8/9/12<br />
<br />
Miniprepped 3mL of each 8/8/12 culture and nanodropped:<br />
<br />
gfpt1 - 155 ng/uL<br />
<br />
gfpt2 - 114 ng/uL<br />
<br />
Digested gfpt1 and gfpt2 with X and P<br />
<br />
Ran on a 1% agarose gel with the digested GFP plasmid<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel080912<br />
<br />
Made glyercol stocks with aliquot of the remaining liquid cultures<br />
<br />
<br />
8/13/12<br />
<br />
Prepared sequencing samples <br />
<br />
*Sample w/ Primer*<br />
GFPT1 w/ VF2<br />
GFPT1 w/ VR<br />
GFPT2 w/ VF2<br />
GFPT2 w/ VR<br />
<br />
200ng of DNA + 16 pmol of primer<br />
<br />
Annealed oligos again GFPT1/2<br />
<br />
Repeated ligation of oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Followed Haynes assembly protocol instead of standard DH5alpha protocol. (http://openwetware.org/wiki/Haynes:Assembly101)<br />
<br />
Transformed ligations into competent DH5alpha<br />
<br />
Plated on amp treated plates<br />
<br />
8/14/12<br />
<br />
Took pictures of plates<br />
<br />
Green-white screened plates<br />
<br />
Picked 4 white colonies from each of gfpt1/2 plates <br />
<br />
Made 5mL LB amp cultures of each colony<br />
<br />
Delivered GFPT1/2 dna samples to biodesign for sequencing (samples from 8/13/12)<br />
<br />
8/15/12<br />
<br />
Miniprepped 3mL of each liquid culture of GFPT1/2<br />
<br />
Prepared glycerol stocks using 100uL of each liquid culture<br />
<br />
Nanodropped samples:<br />
<br />
GFPT1-1 - 172.6 ng/uL<br />
GFPT1-2 - 203.7 ng/uL<br />
GFPT1-3 - 197.4 ng/uL<br />
GFPT1-4 - 178.9 ng/uL<br />
GFPT2-1 - 107.3 ng/uL<br />
GFPT2-2 - 131.2 ng/uL<br />
GFPT2-3 - 145.5 ng/uL<br />
GFPT2-4 - 172.0 ng/uL<br />
<br />
8/17/12 <br />
<br />
GFPT1 sequence confirmed<br />
<br />
Prepared aliquots of GFPT2 minipreps from 8/15/12 for sequencing<br />
<br />
Delivered GFPT2 samples to biodesign for sequencing<br />
<br />
8/19/12 <br />
<br />
GFPT2 sequences confirmed<br />
<br />
<br />
8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
8/29/12<br />
<br />
Discarded GFPT1/2 cultures from 8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
Digested GFPT1/2 with X and S<br />
<br />
Ran a 1% agarose gel with GFPT1/2 digestions <br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel082912<br />
<br />
Cut out inserts and GFPT2 backbone and stored in 4C for gel extraction and tandum repeat assembly experiments<br />
<br />
8/30/12<br />
<br />
Prepared extra glyercol stocks of GFPT1/2 cultures from 8/29/12<br />
<br />
Miniprepped 3mL of each culture, stored at -20C<br />
<br />
9/19/12<br />
<br />
Set up VF2/VR endpoint PCR for double transform minipreps<br />
<br />
1-1, 1-1I, 1-2, 1-2I, 1-3, 1-3I, 2-1, 2-1I, 2-2, 2-2I, 2-3, 2-3I, GFPT1 (positive controls), GFPT2 (positive controls)<br />
<br />
<br />
Annealing temp set to 55C for 25 cycles<br />
<br />
<br />
Resuspended GFPT1 probe and GFPT2 probe oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
9/25/12<br />
<br />
Resuspended pSB1A2 FWD and pSB1A2 REV (amp resistance primers) oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
Prepared 1.6uM dilutions (500uL)<br />
<br />
Did endpoint PCR using pSB1A2 primer pair on<br />
<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG, 1-1, 1-1I, 2-1, 2-1I, GFPT1, GFPT2<br />
<br />
Did endpoint PCR using VF2/VR primer pair on<br />
<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG<br />
<br />
Annealing temp 55C for 25 cycles, stored products at -20C<br />
<br />
Made 3mL liquid cultures of the shipping vector (pSB1C3 with RFP insert) in DH5alpha in chloramphenicol resistant LB<br />
<br />
Made 10mL liquid cultures of:<br />
<br />
Topo in kanamycin<br />
<br />
topo D168A in kanamycin<br />
<br />
topo + GFPT1 in kanamycin + ampicillin<br />
<br />
topo + GFPT2 in kanamycin + ampicillin <br />
<br />
stored @ 37C<br />
<br />
9/26/12<br />
<br />
Picked two new colonies for topo + GFPT2 and grew 10mL cultures of amp+kan LB broth for each<br />
<br />
Ran a gel with:<br />
<br />
Hyperladder I, GFPT1 pSB1A2, GFPT2 pSB1A2, Topo pSB1A2, Topo D168A pSB1A2, Topo D168A VF2/VR, Topo VF2/VR, GFPT1 VF2/VR, GFPT2 VF2/VR, Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092612-1 I ALSO UPDATED THE LIST OF STUFF IN THIS GEL (see line above this one)<br />
<br />
Ran a gel with PCR samples from 9/19 and 9/25:<br />
<br />
Hyperladder I, 1-1, 1-1I, 2-1, 2-1I, 2-1I(VF), 2-1(VF), 1-1I(VF), 1-1(VF), Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092612-2<br />
<br />
Samples in wells 2-5 used the pSB1A2 primer pair. Samples in wells 6-9 used VF2/VR primer pair.<br />
<br />
Revived GFPT1/2 from glycerol stocks (from 8/9 and 8/15) in 5mL LB amp each.<br />
<br />
Nanodropped miniprepped DNA samples:<br />
<br />
pSB1C3 I - 253.75 ng/uL<br />
<br />
pSB1C3 II - 258.38 ng/uL<br />
<br />
Topo - 24.84 ng/uL<br />
<br />
Topo I - 17.33 ng/uL<br />
<br />
Topo D168A - 7.24 ng/uL<br />
<br />
Topo D168A I - 15.62 ng/uL<br />
<br />
1-1 - 16.49 ng/uL<br />
<br />
1-1I - 16.46 ng/uL<br />
<br />
2-1 - 142.05 ng/uL<br />
<br />
2-1I - 16.98 ng/uL<br />
<br />
Picked new colonies from Topo, Topo D168A, Topo + G1, Topo + G2 and made 1mL colonies in their respective medias<br />
<br />
Stored at -37C<br />
<br />
9/27/12<br />
<br />
Prepared serial dilutions of GFPT2 plasmid 1:10, 1:100, 1:1000, 1:10000<br />
<br />
Prepared primer mixes for pSB and VF/VR primers <br />
<br />
Prepared realtime PCR plate, ran RT-qPCR with annealing temp 57<br />
<br />
Miniprepped GFPT1/2 cultures from 9/26<br />
<br />
Nanodropped Miniprepped DNA:<br />
<br />
GFPT1 - 276.12 ng/uL<br />
<br />
GFPT2 - 219.84 ng/uL<br />
<br />
Used 100uL of each 1mL culture from 9/26 to seed 10 mL cultures in their respective media<br />
<br />
Added 10uL of 1M IPTG to each culture ~4 hours after seeding<br />
<br />
Removed cells from 37C ~4 hours after IPTG inducing<br />
<br />
Pelleted and lysed following the bugbuster protocol (http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2012/05/22) (used lysonase for <br />
Topo and Topo D168A only)<br />
<br />
HIS purified proteins using the Zymo HIS purification kit<br />
<br />
<br />
Digested pSB1C3 plasmid with X and P<br />
<br />
Sent protein samples (HIS purified samples from 9/19, 20uL) and ssDNA control (GFPT1/2 probe oligos 20uL @ 10uM) for mass spec<br />
<br />
9/28/12<br />
<br />
Resuspended 8 new oligos, final volume 100 uM each<br />
<br />
Annealed pet29 top/bot oligos<br />
<br />
Redid the RT-PCR, 3x primer concentration, added 1:1 plasmid concentration<br />
<br />
Made 1.6uM aliquots of primer stocks 1-7 (including topo add X primer previously ordered)<br />
<br />
Diluted aliquot of Topo D168A plasmid 4:10<br />
<br />
Diluted PSV plasmid 2:20<br />
<br />
Performed Endpoint PCR on: <br />
<br />
Topo D168A<br />
using primers 1,2 and primers 1,3<br />
<br />
PSV using primers 4-5 and primers 6-7<br />
<br />
4 samples with low primer concentration, 4 samples with twice as much primer (labelled 'H')<br />
<br />
Miniprepped Topo1 2, Topo1 3, Topo1 4 (biobricked Topo D168A without T7, multiple colonies from ligation into shipping vector)<br />
<br />
Nanodropped:<br />
<br />
Topo1 2 - 63.63 ng/uL<br />
<br />
Topo1 3 - 75.07 ng/uL<br />
<br />
Topo1 4 - 184.93 ng/uL<br />
<br />
1mL chloramphenicol cultures of GFPT1/GFPT2 in shipping vector prepared<br />
<br />
<br />
Digested Topo1 2, Topo1 3, Topo1 4 with E and P<br />
<br />
Ran digested Topo plasmids on 1% agarose gel with Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092812<br />
<br />
<br />
Revived cultures of 2xGFPT1, 2xGFPT2, Topo, and Topo D168A (4 mL cultures each in their respective medias)<br />
<br />
T5 exonuclease treated miniprepped plasmids (1-1, 1-1I) <br />
<br />
A1 1-1I + t5<br />
<br />
B1 1-1 + t5<br />
<br />
A2 1-1I untreated<br />
<br />
B2 1-1 untreated<br />
<br />
9/29/12<br />
<br />
Miniprepped GFPT1, GFPT2, Topo, and Topo D168A (Topo cultures separated into 3mL and 1mL minipreps)<br />
<br />
Nanodropped miniprepped DNA:<br />
<br />
GFPT1 I - 275.29 ng/uL<br />
<br />
GFPT1 II - 101.08 ng/uL<br />
<br />
GFPT2 I - 222.11 ng/uL<br />
<br />
GFPT2 II - 230.52 ng/uL<br />
<br />
Topo I - 117.97 ng/uL<br />
<br />
Topo II - 70.28 ng/uL<br />
<br />
Topo D168A I - 69.47 ng/uL<br />
<br />
Topo D168A II - 51.87 ng/uL<br />
<br />
HIS purified crude lysates from 9/27/12 (Topo, Topo D168A, Topo + GFPT1, Topo + GFPT2, all IPTG induced)<br />
<br />
Digested Topo I plasmid (pet29a) with E and X <br />
<br />
Ran a gel with Hyperladder I, 1-2 1-3 4-5 and 6-7 PCR products, pet29a digestion, and pSB1C3 digestion<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE "ASUiGEM2012_gel092912 fragment pcr confirmation"<br />
<br />
Confirmed that PCR made amplicons <br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092912-1<br />
<br />
Excised bands for digested pet29a and pSB1C3 plasmids<br />
<br />
Gel extracted 4 gel fragments (2 wells per sample: digested pSB1C3 plasmid, digested pet29a plasmid)<br />
<br />
Nanodropped gel extractions:<br />
<br />
Digested pSB1C3 - 25.54 ng/uL<br />
<br />
Digested pet29a - 22.95 ng/uL<br />
<br />
Set up a bradford assay of topo, topo D168A, topo + G1, topo + G2 (5uL protein, 10uL protein, 20uL protein + 200uL reagent)<br />
<br />
Used ~100uL aliquot of BL21 competent glycerol stock to seed 10mL of LB medium (no antibiotic), stored at 37C<br />
<br />
Ran 1% agarose gel with samples: Hyperladder I, A1, B1, A2, B2 and Hyperladder I alpha, s+l+a 1a,s+l+a 2c,s+l+a (2b)strep samples<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel092912 alpha, s+l+a 1a,s+l+a 2c,s+l+a (2b)-2<br />
<br />
Did bug buster protocol to lyse BL21 control culture (used lysonase)<br />
<br />
Ligated digested pet29a with pet29 top/bot annealed oligos<br />
<br />
Transformed ligation using invitrogen DH5alpha transformation protocol<br />
<br />
Prepared LB kanamycin plates<br />
<br />
plated transformed ligations on prewarmed kanamycin plates<br />
<br />
9/30/12<br />
<br />
Pet29a plates did not grow<br />
<br />
Kinase treated pet29a oligos<br />
<br />
Annealed kinase treated pet29a oligos<br />
<br />
Ligated digested pet29a (from gel extraction) with kinase treated oligos<br />
<br />
Transformed ligations into DH5alpha, used topo plasmid as a positive control<br />
<br />
Plated transformations on kanamycin plates and stored overnight at 37C<br />
<br />
HIS purified BL21 control crude lysate<br />
<br />
Set up a bradford assay with:<br />
<br />
uninduced & induced topo protein extractions from 9/19<br />
<br />
uninduced & induced topo D168A protein extractions from 9/19<br />
<br />
BL21 control lysate<br />
<br />
Topo, Topo D168A, Topo + G1, Topo + G2 from 9/27 <br />
<br />
All samples prepared (10uL protein, 20uL protein + 200uL reagent)<br />
<br />
Miniprepped GFPT1 1,2,3 and GFPT2 (17,18,26) (~600uL of each) (1mL liquid cultures made from colonies on the chloramphenicol plates of GFPT1 and GFPT2 ligated into the shipping vector)<br />
<br />
Nanodropped:<br />
<br />
GFPT1-1 - 38.5 ng/uL<br />
<br />
GFPT1-2 - 77.6 ng/uL<br />
<br />
GFPT1-3 - 74.8 ng/uL<br />
<br />
GFPT2-17 - 61.6 ng/uL<br />
<br />
GFPT2-18 - 65.9 ng/uL<br />
<br />
GFPT2-26 - 64.2 ng/uL<br />
<br />
Ran a 1% agarose gel with 1-1, 1-2, 1-3, 2-17, 2-18, 2-26 plasmid miniprep samples and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel093012-1<br />
<br />
Prepared a 1:2 dilution of GFPT2 plasmid from 9/27 miniprep <br />
<br />
Treated 5uL of diluted plasmid with 5uL of water, BL21 protein, topo protein, topo D168A protein<br />
<br />
Incubated 30 minutes at 37C<br />
<br />
Ran a 1% agarose gel with protein treated target plasmid samples and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM2012_gel093012-2<br />
<br />
Digested GFPT2 plasmid with X (let run at 37C for 30 minutes)<br />
<br />
Used DNA clean up kit on digested GFPT2 <br />
<br />
Nanodropped digested GFPT2:<br />
<br />
GFPT2(X) - 39.85 ng/uL<br />
<br />
Prepared DNA seq samples using VF2 and VR<br />
<br />
Sample# - PrimerPair - DNA sample (sample 1, GFPT2 uncut + VF2; sample 2, GFPT2 uncut + VR)<br />
<br />
1/2 - FWD/REV - uncut GFPT2 plasmid<br />
<br />
3/4 - FWD/REV - cut GFPT2 plamid<br />
<br />
5/6 - FWD/REV - 2:1 uncut:cut GFPT2 plasmid mixture<br />
<br />
7/8 - FWD/REV - 1:1 uncut:cut GFPT2 plasmid mixture<br />
<br />
9/10 - FWD/REV - 1:2 uncut:cut GFPT2 plasmid mixture<br />
<br />
11/12 - FWD/REV - 1-2 miniprep sample from 9/19 double transformations<br />
<br />
13/14 - FWD/REV - 1-2I <br />
<br />
15/16 - FWD/REV - 1-3<br />
<br />
17/18 - FWD/REV - 1-3I<br />
<br />
19/20 - FWD/REV - 2-1<br />
<br />
21/22 - FWD/REV - 2-1I<br />
<br />
23/24 - FWD/REV - 2-2<br />
<br />
25/26 - FWD/REV - 2-2I<br />
<br />
27/28 - FWD/REV - 2-3<br />
<br />
29/30 - FWD/REV - 2-3I<br />
<br />
10/2/12<br />
<br />
Prepared PCR tubes with 10uL Topo1 4 (25ng/uL topo d168a in the shipping vector), 10uL GFPT2-26 (25ng/uL GFPT2 in the shipping vector), and 10uL GFPT1-3 (25ng/uL GFPT1 in the shipping vector)<br />
<br />
Labelled the tubes K891234, K891999, K891000 respectively and shipped overnight to iGEM Headquarters<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE BELOW THIS IS NEW<br />
<br />
Topo D168A treated DNA samples, incubated for 10 minutes at 37C:<br />
<br />
Omega fragment PCR amplicon<br />
<br />
Topo coding sequence PCR<br />
<br />
GFPT1 VF2/VR PCR amplicon<br />
<br />
Ran a 1% agarose gel containing untreated omega PCR, untreated topo PCR, untreated GFPT1 PCR, treated omega PCR, treated topo PCR, treated GFPT1 PCR, and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE ASUiGEM_gel100212<br />
<br />
10/3/12<br />
<br />
Protein treated GFPT2 plasmid, incubated for 10 minutes at 37C:<br />
<br />
BL21 HIS-purified Control Lysate<br />
<br />
Topo<br />
<br />
Topo D168A<br />
<br />
Ran a 1% agarose gel containing GFPT1, GFPT1 + BL21 protein, GFPT1 + Topo, GFPT1 + Topo D168A, Topo D168A (no DNA), and Hyperladder I<br />
<br />
///////////////////////////////////////////////////////////ROHIT LOOK HERE Photo10031712 annotated</div>
Hyder
http://2012.igem.org/Team:Arizona_State/ssDNA
Team:Arizona State/ssDNA
2012-10-03T20:55:37Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<br />
The wild type form of topoisomerase binds to the DNA sequence (YCCTT) in E. Coli. It regulates the winding of the DNA by making a nick after the second T. This allows for the rotation of the strands to relieve torsional stress. Afterwards, the DNA strands are religated. In 2006, Bushman et al. have shown that the smallpox topoisomerase double cysteine mutant D168A mutates the tyrosine responsible for covalent bonding to the 5’ phosphate at the DNA nicking. This mutant form prevents religation, and thus causes the majority of the DNA to stay in the covalently bonded complex.<br />
<br />
<br />
In our design, we plan to use topoisomerase to nick a specific covalently bonded sequence and peel off a section of single stranded DNA. We have designed a template plasmid that includes tandem YCCTT recognition sites with template strand in between, and is complementary to a section of coding sequence of GFP. We plan to use a KEIO strain with one copy of this coding sequence in the E. Coli genome.<br />
<br />
<br />
Basilion et al. from Case Western in 2010 have shown that they were able to make a split beta-galactosidase complementation assay with relatively reliable assay results In the assay, alpha-4/omega, which has a higher specificity, is the most successful split beta galactosidase assay. It is thus used to eliminate false positive. Additionally, we are adapting alpha and 1-omega, which is less specific but has a higher signal, for the same protocol to eliminate false negative.<br />
<br />
<br />
Additionally, Basilion et al. also demonstrated success in creating fusion proteins with a split-beta galactosidase fragment and antibody specific to their target. Modifying this, we plan to make a fusion of our mutant topoisomerase and our split-beta galactosidase fragments. This effectively creates a probe that when assembled contains topoisomerase bound both to a single stranded DNA hybridization probe and a split-beta galactosidase fragment. By incubating the two probes that recognize adjacent DNA sequences, we can test for the presence of DNA sequences in a bacterial genome.</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-03T02:55:29Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12<br />
<br />
Picked the colonies again and started new liquid cultures (5mL LB amp).<br />
<br />
Discarded cultures for 8/7/12<br />
<br />
8/9/12<br />
<br />
Miniprepped 3mL of each 8/8/12 culture and nanodropped:<br />
<br />
gfpt1 - 155 ng/uL<br />
<br />
gfpt2 - 114 ng/uL<br />
<br />
Digested gfpt1 and gfpt2 with X and P<br />
<br />
Ran on a 1% agarose gel with the digested GFP plasmid<br />
<br />
Made glyercol stocks with aliquot of the remaining liquid cultures<br />
<br />
<br />
8/13/12<br />
<br />
Prepared sequencing samples <br />
<br />
*Sample w/ Primer*<br />
GFPT1 w/ VF2<br />
GFPT1 w/ VR<br />
GFPT2 w/ VF2<br />
GFPT2 w/ VR<br />
<br />
200ng of DNA + 16 pmol of primer<br />
<br />
Annealed oligos again GFPT1/2<br />
<br />
Repeated ligation of oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Followed Haynes assembly protocol instead of standard DH5alpha protocol. (http://openwetware.org/wiki/Haynes:Assembly101)<br />
<br />
Transformed ligations into competent DH5alpha<br />
<br />
Plated on amp treated plates<br />
<br />
8/14/12<br />
<br />
Took pictures of plates<br />
<br />
Green-white screened plates<br />
<br />
Picked 4 white colonies from each of gfpt1/2 plates <br />
<br />
Made 5mL LB amp cultures of each colony<br />
<br />
Delivered GFPT1/2 dna samples to biodesign for sequencing (samples from 8/13/12)<br />
<br />
8/15/12<br />
<br />
Miniprepped 3mL of each liquid culture of GFPT1/2<br />
<br />
Prepared glycerol stocks using 100uL of each liquid culture<br />
<br />
Nanodropped samples:<br />
<br />
GFPT1-1 - 172.6 ng/uL<br />
GFPT1-2 - 203.7 ng/uL<br />
GFPT1-3 - 197.4 ng/uL<br />
GFPT1-4 - 178.9 ng/uL<br />
GFPT2-1 - 107.3 ng/uL<br />
GFPT2-2 - 131.2 ng/uL<br />
GFPT2-3 - 145.5 ng/uL<br />
GFPT2-4 - 172.0 ng/uL<br />
<br />
8/17/12 <br />
<br />
GFPT1 sequence confirmed<br />
<br />
Prepared aliquots of GFPT2 minipreps from 8/15/12 for sequencing<br />
<br />
Delivered GFPT2 samples to biodesign for sequencing<br />
<br />
8/19/12 <br />
<br />
GFPT2 sequences confirmed<br />
<br />
<br />
8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
8/29/12<br />
<br />
Discarded GFPT1/2 cultures from 8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
Digested GFPT1/2 with X and S<br />
<br />
Ran a 1% agarose gel with GFPT1/2 digestions <br />
<br />
Cut out inserts and GFPT2 backbone and stored in 4C for gel extraction and tandum repeat assembly experiments<br />
<br />
8/30/12<br />
<br />
Prepared extra glyercol stocks of GFPT1/2 cultures from 8/29/12<br />
<br />
Miniprepped 3mL of each culture, stored at -20C<br />
<br />
9/19/12<br />
<br />
Set up VF2/VR endpoint PCR for double transform minipreps<br />
<br />
1-1, 1-1I, 1-2, 1-2I, 1-3, 1-3I, 2-1, 2-1I, 2-2, 2-2I, 2-3, 2-3I, GFPT1 (positive controls), GFPT2 (positive controls)<br />
<br />
<br />
Annealing temp set to 55C for 25 cycles<br />
<br />
<br />
Resuspended GFPT1 probe and GFPT2 probe oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
9/25/12<br />
<br />
Resuspended pSB1A2 FWD and pSB1A2 REV (amp resistance primers) oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
Prepared 1.6uM dilutions (500uL)<br />
<br />
Did endpoint PCR using pSB1A2 primer pair on<br />
<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG, 1-1, 1-1I, 2-1, 2-1I, GFPT1, GFPT2<br />
<br />
Did endpoint PCR using VF2/VR primer pair on<br />
<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG<br />
<br />
Annealing temp 55C for 25 cycles, stored products at -20C<br />
<br />
Made 3mL liquid cultures of the shipping vector (pSB1C3 with RFP insert) in DH5alpha in chloramphenicol resistant LB<br />
<br />
Made 10mL liquid cultures of:<br />
<br />
Topo in kanamycin<br />
<br />
topo D168A in kanamycin<br />
<br />
topo + GFPT1 in kanamycin + ampicillin<br />
<br />
topo + GFPT2 in kanamycin + ampicillin <br />
<br />
stored @ 37C<br />
<br />
9/26/12<br />
<br />
Picked two new colonies for topo + GFPT2 and grew 10mL cultures of amp+kan LB broth for each<br />
<br />
Ran a gel with:<br />
<br />
Hyperladder I, GFPT1 pSB1A2, GFPT2 pSB1A2, Topo pSB1A2, Topo D168A<br />
<br />
Ran a gel with PCR samples from 9/19 and 9/25:<br />
<br />
Hyperladder I, 1-1, 1-1I, 2-1, 2-1I, 2-1I(VF), 2-1(VF), 1-1I(VF), 1-1(VF), Hyperladder I<br />
<br />
Samples in wells 2-5 used the pSB1A2 primer pair. Samples in wells 6-9 used VF2/VR primer pair.<br />
<br />
Revived GFPT1/2 from glycerol stocks (from 8/9 and 8/15) in 5mL LB amp each.<br />
<br />
Nanodropped miniprepped DNA samples:<br />
<br />
pSB1C3 I - 253.75 ng/uL<br />
<br />
pSB1C3 II - 258.38 ng/uL<br />
<br />
Topo - 24.84 ng/uL<br />
<br />
Topo I - 17.33 ng/uL<br />
<br />
Topo D168A - 7.24 ng/uL<br />
<br />
Topo D168A I - 15.62 ng/uL<br />
<br />
1-1 - 16.49 ng/uL<br />
<br />
1-1I - 16.46 ng/uL<br />
<br />
2-1 - 142.05 ng/uL<br />
<br />
2-1I - 16.98 ng/uL<br />
<br />
Picked new colonies from Topo, Topo D168A, Topo + G1, Topo + G2 and made 1mL colonies in their respective medias<br />
<br />
Stored at -37C<br />
<br />
9/27/12<br />
<br />
Prepared serial dilutions of GFPT2 plasmid 1:10, 1:100, 1:1000, 1:10000<br />
<br />
Prepared primer mixes for pSB and VF/VR primers <br />
<br />
Prepared realtime PCR plate, ran RT-qPCR with annealing temp 57<br />
<br />
Miniprepped GFPT1/2 cultures from 9/26<br />
<br />
Nanodropped Miniprepped DNA:<br />
<br />
GFPT1 - 276.12 ng/uL<br />
<br />
GFPT2 - 219.84 ng/uL<br />
<br />
Used 100uL of each 1mL culture from 9/26 to seed 10 mL cultures in their respective media<br />
<br />
Added 10uL of 1M IPTG to each culture ~4 hours after seeding<br />
<br />
Removed cells from 37C ~4 hours after IPTG inducing<br />
<br />
Pelleted and lysed following the bugbuster protocol (http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2012/05/22) (used lysonase for <br />
Topo and Topo D168A only)<br />
<br />
HIS purified proteins using the Zymo HIS purification kit<br />
<br />
<br />
Digested pSB1C3 plasmid with X and P<br />
<br />
Sent protein samples (HIS purified samples from 9/19, 20uL) and ssDNA control (GFPT1/2 probe oligos 20uL @ 10uM) for mass spec<br />
<br />
9/28/12<br />
<br />
Resuspended 8 new oligos, final volume 100 uM each<br />
<br />
Annealed pet29 top/bot oligos<br />
<br />
Redid the RT-PCR, 3x primer concentration, added 1:1 plasmid concentration<br />
<br />
Made 1.6uM aliquots of primer stocks 1-7 (including topo add X primer previously ordered)<br />
<br />
Diluted aliquot of Topo D168A plasmid 4:10<br />
<br />
Diluted PSV plasmid 2:20<br />
<br />
Performed Endpoint PCR on: <br />
<br />
Topo D168A<br />
using primers 1,2 and primers 1,3<br />
<br />
PSV using primers 4-5 and primers 6-7<br />
<br />
4 samples with low primer concentration, 4 samples with twice as much primer (labelled 'H')<br />
<br />
Miniprepped Topo1 2, Topo1 3, Topo1 4 (biobricked Topo D168A without T7, multiple colonies from ligation into shipping vector)<br />
<br />
Nanodropped:<br />
<br />
Topo1 2 - 63.63 ng/uL<br />
<br />
Topo1 3 - 75.07 ng/uL<br />
<br />
Topo1 4 - 184.93 ng/uL<br />
<br />
1mL chloramphenicol cultures of GFPT1/GFPT2 in shipping vector prepared<br />
<br />
<br />
Digested Topo1 2, Topo1 3, Topo1 4 with E and P<br />
<br />
Ran digested Topo plasmids on 1% agarose gel with Hyperladder I<br />
<br />
<br />
<br />
<br />
Revived cultures of 2xGFPT1, 2xGFPT2, Topo, and Topo D168A (4 mL cultures each in their respective medias)<br />
<br />
T5 exonuclease treated miniprepped plasmids (1-1, 1-1I) <br />
<br />
A1 1-1I + t5<br />
<br />
B1 1-1 + t5<br />
<br />
A2 1-1I untreated<br />
<br />
B2 1-1 untreated<br />
<br />
9/29/12<br />
<br />
Miniprepped GFPT1, GFPT2, Topo, and Topo D168A (Topo cultures separated into 3mL and 1mL minipreps)<br />
<br />
Nanodropped miniprepped DNA:<br />
<br />
GFPT1 I - 275.29 ng/uL<br />
<br />
GFPT1 II - 101.08 ng/uL<br />
<br />
GFPT2 I - 222.11 ng/uL<br />
<br />
GFPT2 II - 230.52 ng/uL<br />
<br />
Topo I - 117.97 ng/uL<br />
<br />
Topo II - 70.28 ng/uL<br />
<br />
Topo D168A I - 69.47 ng/uL<br />
<br />
Topo D168A II - 51.87 ng/uL<br />
<br />
HIS purified crude lysates from 9/27/12 (Topo, Topo D168A, Topo + GFPT1, Topo + GFPT2, all IPTG induced)<br />
<br />
Digested Topo I plasmid (pet29a) with E and X <br />
<br />
Ran a gel with Hyperladder I, 1-2 1-3 4-5 and 6-7 PCR products, pet29a digestion, and pSB1C3 digestion<br />
<br />
Confirmed that PCR made amplicons <br />
<br />
Excised bands for digested pet29a and pSB1C3 plasmids<br />
<br />
Gel extracted 4 gel fragments (2 wells per sample: digested pSB1C3 plasmid, digested pet29a plasmid)<br />
<br />
Nanodropped gel extractions:<br />
<br />
Digested pSB1C3 - 25.54 ng/uL<br />
<br />
Digested pet29a - 22.95 ng/uL<br />
<br />
Set up a bradford assay of topo, topo D168A, topo + G1, topo + G2 (5uL protein, 10uL protein, 20uL protein + 200uL reagent)<br />
<br />
Used ~100uL aliquot of BL21 competent glycerol stock to seed 10mL of LB medium (no antibiotic), stored at 37C<br />
<br />
Ran 1% agarose gel with samples: A1, B1, A2, B2 and Hyperladder I<br />
<br />
Did bug buster protocol to lyse BL21 control culture (used lysonase)<br />
<br />
Ligated digested pet29a with pet29 top/bot annealed oligos<br />
<br />
Transformed ligation using invitrogen DH5alpha transformation protocol<br />
<br />
Prepared LB kanamycin plates<br />
<br />
plated transformed ligations on prewarmed kanamycin plates<br />
<br />
9/30/12<br />
<br />
Pet29a plates did not grow<br />
<br />
Kinase treated pet29a oligos<br />
<br />
Annealed kinase treated pet29a oligos<br />
<br />
Ligated digested pet29a (from gel extraction) with kinase treated oligos<br />
<br />
Transformed ligations into DH5alpha, used topo plasmid as a positive control<br />
<br />
Plated transformations on kanamycin plates and stored overnight at 37C<br />
<br />
HIS purified BL21 control crude lysate<br />
<br />
Set up a bradford assay with:<br />
<br />
uninduced & induced topo protein extractions from 9/19<br />
<br />
uninduced & induced topo D168A protein extractions from 9/19<br />
<br />
BL21 control lysate<br />
<br />
Topo, Topo D168A, Topo + G1, Topo + G2 from 9/27 <br />
<br />
All samples prepared (10uL protein, 20uL protein + 200uL reagent)<br />
<br />
Miniprepped GFPT1 1,2,3 and GFPT2 (17,18,26) (~600uL of each) (1mL liquid cultures made from colonies on the chloramphenicol plates of GFPT1 and GFPT2 ligated into the shipping vector)<br />
<br />
Nanodropped:<br />
<br />
GFPT1-1 - 38.5 ng/uL<br />
<br />
GFPT1-2 - 77.6 ng/uL<br />
<br />
GFPT1-3 - 74.8 ng/uL<br />
<br />
GFPT2-17 - 61.6 ng/uL<br />
<br />
GFPT2-18 - 65.9 ng/uL<br />
<br />
GFPT2-26 - 64.2 ng/uL<br />
<br />
Ran a 1% agarose gel with 1-1, 1-2, 1-3, 2-17, 2-18, 2-26 plasmid miniprep samples and Hyperladder I<br />
<br />
Prepared a 1:2 dilution of GFPT2 plasmid from 9/27 miniprep <br />
<br />
Treated 5uL of diluted plasmid with 5uL of water, BL21 protein, topo protein, topo D168A protein<br />
<br />
Incubated 30 minutes at 37C<br />
<br />
Ran a 1% agarose gel with protein treated target plasmid samples and Hyperladder I<br />
<br />
Digested GFPT2 plasmid with X (let run at 37C for 30 minutes)<br />
<br />
Used DNA clean up kit on digested GFPT2 <br />
<br />
Nanodropped digested GFPT2:<br />
<br />
GFPT2(X) - 39.85 ng/uL<br />
<br />
Prepared DNA seq samples using VF2 and VR<br />
<br />
Sample# - PrimerPair - DNA sample (sample 1, GFPT2 uncut + VF2; sample 2, GFPT2 uncut + VR)<br />
<br />
1/2 - FWD/REV - uncut GFPT2 plasmid<br />
<br />
3/4 - FWD/REV - cut GFPT2 plamid<br />
<br />
5/6 - FWD/REV - 2:1 uncut:cut GFPT2 plasmid mixture<br />
<br />
7/8 - FWD/REV - 1:1 uncut:cut GFPT2 plasmid mixture<br />
<br />
9/10 - FWD/REV - 1:2 uncut:cut GFPT2 plasmid mixture<br />
<br />
11/12 - FWD/REV - 1-2 miniprep sample from 9/19 double transformations<br />
<br />
13/14 - FWD/REV - 1-2I <br />
<br />
15/16 - FWD/REV - 1-3<br />
<br />
17/18 - FWD/REV - 1-3I<br />
<br />
19/20 - FWD/REV - 2-1<br />
<br />
21/22 - FWD/REV - 2-1I<br />
<br />
23/24 - FWD/REV - 2-2<br />
<br />
25/26 - FWD/REV - 2-2I<br />
<br />
27/28 - FWD/REV - 2-3<br />
<br />
29/30 - FWD/REV - 2-3I<br />
<br />
10/2/12<br />
<br />
Prepared PCR tubes with 10uL Topo1 4 (25ng/uL topo d168a in the shipping vector), 10uL GFPT2-26 (25ng/uL GFPT2 in the shipping vector), and 10uL GFPT1-3 (25ng/uL GFPT1 in the shipping vector)<br />
<br />
Labelled the tubes K891234, K891999, K891000 respectively and shipped overnight to iGEM Headquarters</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-02T23:57:29Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:100px;" /><br />
</a><br />
</td><br />
<td><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:675px;" /></p><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="125" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Overview">Overview</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin"><s>Magainin</s></a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">Chimeric Reporter</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA"><s>ssDNA</s></a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Judging_Criteria">Judging Criteria</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Field Applications</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Arizona Community</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">Our University</a> </li> <br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">Parts Submitted to the Registry</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-02T23:56:38Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td valign = "bottom"><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:100px;" /><br />
</a><br />
</td><br />
<td valign = "top"><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:675px;" /></p><br />
</a><br />
</td><br />
<td valign = "bottom"><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="125" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Overview">Overview</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin"><s>Magainin</s></a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">Chimeric Reporter</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA"><s>ssDNA</s></a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Judging_Criteria">Judging Criteria</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Field Applications</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Arizona Community</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">Our University</a> </li> <br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">Parts Submitted to the Registry</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-02T23:51:25Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:80px;" /><br />
</a><br />
</td><br />
<td><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:750px;" /></p><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="145" height="80" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Overview">Overview</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin"><s>Magainin</s></a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">Chimeric Reporter</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA"><s>ssDNA</s></a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Judging_Criteria">Judging Criteria</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Field Applications</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Arizona Community</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">Our University</a> </li> <br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">Parts Submitted to the Registry</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-10-02T23:48:29Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</td><br />
<td><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2012/9/9a/AsuCrsysLogothingy.png" style="width:550px;" /></p><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="175" height="80" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Overview">Overview</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin"><s>Magainin</s></a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">Chimeric Reporter</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA"><s>ssDNA</s></a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Judging_Criteria">Judging Criteria</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Field Applications</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Arizona Community</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">Our University</a> </li> <br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">Parts Submitted to the Registry</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/File:AsuCrsysLogothingy.png
File:AsuCrsysLogothingy.png
2012-10-02T23:47:48Z
<p>Hyder: </p>
<hr />
<div></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-01T10:34:45Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12<br />
<br />
Picked the colonies again and started new liquid cultures (5mL LB amp).<br />
<br />
Discarded cultures for 8/7/12<br />
<br />
8/9/12<br />
<br />
Miniprepped 3mL of each 8/8/12 culture and nanodropped:<br />
<br />
gfpt1 - 155 ng/uL<br />
<br />
gfpt2 - 114 ng/uL<br />
<br />
Digested gfpt1 and gfpt2 with X and P<br />
<br />
Ran on a 1% agarose gel with the digested GFP plasmid<br />
<br />
Made glyercol stocks with aliquot of the remaining liquid cultures<br />
<br />
<br />
8/13/12<br />
<br />
Prepared sequencing samples <br />
<br />
*Sample w/ Primer*<br />
GFPT1 w/ VF2<br />
GFPT1 w/ VR<br />
GFPT2 w/ VF2<br />
GFPT2 w/ VR<br />
<br />
200ng of DNA + 16 pmol of primer<br />
<br />
Annealed oligos again GFPT1/2<br />
<br />
Repeated ligation of oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Followed Haynes assembly protocol instead of standard DH5alpha protocol. (http://openwetware.org/wiki/Haynes:Assembly101)<br />
<br />
Transformed ligations into competent DH5alpha<br />
<br />
Plated on amp treated plates<br />
<br />
8/14/12<br />
<br />
Took pictures of plates<br />
<br />
Green-white screened plates<br />
<br />
Picked 4 white colonies from each of gfpt1/2 plates <br />
<br />
Made 5mL LB amp cultures of each colony<br />
<br />
Delivered GFPT1/2 dna samples to biodesign for sequencing (samples from 8/13/12)<br />
<br />
8/15/12<br />
<br />
Miniprepped 3mL of each liquid culture of GFPT1/2<br />
<br />
Prepared glycerol stocks using 100uL of each liquid culture<br />
<br />
Nanodropped samples:<br />
<br />
GFPT1-1 - 172.6 ng/uL<br />
GFPT1-2 - 203.7 ng/uL<br />
GFPT1-3 - 197.4 ng/uL<br />
GFPT1-4 - 178.9 ng/uL<br />
GFPT2-1 - 107.3 ng/uL<br />
GFPT2-2 - 131.2 ng/uL<br />
GFPT2-3 - 145.5 ng/uL<br />
GFPT2-4 - 172.0 ng/uL<br />
<br />
8/17/12 <br />
<br />
GFPT1 sequence confirmed<br />
<br />
Prepared aliquots of GFPT2 minipreps from 8/15/12 for sequencing<br />
<br />
Delivered GFPT2 samples to biodesign for sequencing<br />
<br />
8/19/12 <br />
<br />
GFPT2 sequences confirmed<br />
<br />
<br />
8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
8/29/12<br />
<br />
Discarded GFPT1/2 cultures from 8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
Digested GFPT1/2 with X and S<br />
<br />
Ran a 1% agarose gel with GFPT1/2 digestions <br />
<br />
Cut out inserts and GFPT2 backbone and stored in 4C for gel extraction and tandum repeat assembly experiments<br />
<br />
8/30/12<br />
<br />
Prepared extra glyercol stocks of GFPT1/2 cultures from 8/29/12<br />
<br />
Miniprepped 3mL of each culture, stored at -20C<br />
<br />
9/19/12<br />
<br />
Set up VF2/VR endpoint PCR for double transform minipreps<br />
<br />
1-1, 1-1I, 1-2, 1-2I, 1-3, 1-3I, 2-1, 2-1I, 2-2, 2-2I, 2-3, 2-3I, GFPT1 (positive controls), GFPT2 (positive controls)<br />
<br />
<br />
Annealing temp set to 55C for 25 cycles<br />
<br />
<br />
Resuspended GFPT1 probe and GFPT2 probe oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
9/25/12<br />
<br />
Resuspended pSB1A2 FWD and pSB1A2 REV (amp resistance primers) oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
Prepared 1.6uM dilutions (500uL)<br />
<br />
Did endpoint PCR using pSB1A2 primer pair on<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG, 1-1, 1-1I, 2-1, 2-1I, GFPT1, GFPT2<br />
<br />
Did endpoint PCR using VF2/VR primer pair on<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG<br />
<br />
Annealing temp 55C for 25 cycles, stored products at -20C<br />
<br />
Made 3mL liquid cultures of the shipping vector (pSB1C3 with RFP insert) in DH5alpha in chloramphenicol resistant LB<br />
<br />
Made 10mL liquid cultures of:<br />
Topo in kanamycin<br />
topo D168A in kanamycin<br />
topo + GFPT1 in kanamycin + ampicillin<br />
topo + GFPT2 in kanamycin + ampicillin <br />
<br />
stored @ 37C<br />
<br />
9/26/12<br />
<br />
Picked two new colonies for topo + GFPT2 and grew 10mL cultures of amp+kan LB broth for each<br />
<br />
Ran a gel with:<br />
Hyperladder I, GFPT1 pSB1A2, GFPT2 pSB1A2, Topo pSB1A2, Topo D168A</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-01T09:04:48Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12<br />
<br />
Picked the colonies again and started new liquid cultures (5mL LB amp).<br />
<br />
Discarded cultures for 8/7/12<br />
<br />
8/9/12<br />
<br />
Miniprepped 3mL of each 8/8/12 culture and nanodropped:<br />
<br />
gfpt1 - 155 ng/uL<br />
<br />
gfpt2 - 114 ng/uL<br />
<br />
Digested gfpt1 and gfpt2 with X and P<br />
<br />
Ran on a 1% agarose gel with the digested GFP plasmid<br />
<br />
Made glyercol stocks with aliquot of the remaining liquid cultures<br />
<br />
<br />
8/13/12<br />
<br />
Prepared sequencing samples <br />
<br />
*Sample w/ Primer*<br />
GFPT1 w/ VF2<br />
GFPT1 w/ VR<br />
GFPT2 w/ VF2<br />
GFPT2 w/ VR<br />
<br />
200ng of DNA + 16 pmol of primer<br />
<br />
Annealed oligos again GFPT1/2<br />
<br />
Repeated ligation of oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Followed Haynes assembly protocol instead of standard DH5alpha protocol. (http://openwetware.org/wiki/Haynes:Assembly101)<br />
<br />
Transformed ligations into competent DH5alpha<br />
<br />
Plated on amp treated plates<br />
<br />
8/14/12<br />
<br />
Took pictures of plates<br />
<br />
Green-white screened plates<br />
<br />
Picked 4 white colonies from each of gfpt1/2 plates <br />
<br />
Made 5mL LB amp cultures of each colony<br />
<br />
Delivered GFPT1/2 dna samples to biodesign for sequencing (samples from 8/13/12)<br />
<br />
8/15/12<br />
<br />
Miniprepped 3mL of each liquid culture of GFPT1/2<br />
<br />
Prepared glycerol stocks using 100uL of each liquid culture<br />
<br />
Nanodropped samples:<br />
<br />
GFPT1-1 - 172.6 ng/uL<br />
GFPT1-2 - 203.7 ng/uL<br />
GFPT1-3 - 197.4 ng/uL<br />
GFPT1-4 - 178.9 ng/uL<br />
GFPT2-1 - 107.3 ng/uL<br />
GFPT2-2 - 131.2 ng/uL<br />
GFPT2-3 - 145.5 ng/uL<br />
GFPT2-4 - 172.0 ng/uL<br />
<br />
8/17/12 <br />
<br />
GFPT1 sequence confirmed<br />
<br />
Prepared aliquots of GFPT2 minipreps from 8/15/12 for sequencing<br />
<br />
Delivered GFPT2 samples to biodesign for sequencing<br />
<br />
8/19/12 <br />
<br />
GFPT2 sequences confirmed<br />
<br />
<br />
8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
8/29/12<br />
<br />
Discarded GFPT1/2 cultures from 8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
Digested GFPT1/2 with X and S<br />
<br />
Ran a 1% agarose gel with GFPT1/2 digestions <br />
<br />
Cut out inserts and GFPT2 backbone and stored in 4C for gel extraction and tandum repeat assembly experiments<br />
<br />
8/30/12<br />
<br />
Prepared extra glyercol stocks of GFPT1/2 cultures from 8/29/12<br />
<br />
Miniprepped 3mL of each culture, stored at -20C<br />
<br />
9/19/12<br />
<br />
Set up VF2/VR endpoint PCR for double transform minipreps<br />
<br />
1-1, 1-1I, 1-2, 1-2I, 1-3, 1-3I, 2-1, 2-1I, 2-2, 2-2I, 2-3, 2-3I, GFPT1 (positive controls), GFPT2 (positive controls)<br />
<br />
<br />
Annealing temp set to 55C for 25 cycles<br />
<br />
<br />
Resuspended GFPT1 probe and GFPT2 probe oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
9/25/12<br />
<br />
Resuspended pSB1A2 FWD and pSB1A2 REV (amp resistance primers) oligos in molecular grade H2O (Final concentration: 100uM), stored at -20C<br />
<br />
Prepared 1.6uM dilutions (500uL)<br />
<br />
Did endpoint PCR using pSB1A2 primer pair on<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG, 1-1, 1-1I, 2-1, 2-1I, GFPT1, GFPT2<br />
<br />
Did endpoint PCR using VF2/VR primer pair on<br />
Topo, Topo IPTG, Topo D168A, Topo D168A IPTG<br />
<br />
Annealing temp 55C for 25 cycles, stored products at -20C</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-01T08:32:56Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12<br />
<br />
Picked the colonies again and started new liquid cultures (5mL LB amp).<br />
<br />
Discarded cultures for 8/7/12<br />
<br />
8/9/12<br />
<br />
Miniprepped 3mL of each 8/8/12 culture and nanodropped:<br />
<br />
gfpt1 - 155 ng/uL<br />
<br />
gfpt2 - 114 ng/uL<br />
<br />
Digested gfpt1 and gfpt2 with X and P<br />
<br />
Ran on a 1% agarose gel with the digested GFP plasmid<br />
<br />
Made glyercol stocks with aliquot of the remaining liquid cultures<br />
<br />
<br />
8/13/12<br />
<br />
Prepared sequencing samples <br />
<br />
*Sample w/ Primer*<br />
GFPT1 w/ VF2<br />
GFPT1 w/ VR<br />
GFPT2 w/ VF2<br />
GFPT2 w/ VR<br />
<br />
200ng of DNA + 16 pmol of primer<br />
<br />
Annealed oligos again GFPT1/2<br />
<br />
Repeated ligation of oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Followed Haynes assembly protocol instead of standard DH5alpha protocol. (http://openwetware.org/wiki/Haynes:Assembly101)<br />
<br />
Transformed ligations into competent DH5alpha<br />
<br />
Plated on amp treated plates<br />
<br />
8/14/12<br />
<br />
Took pictures of plates<br />
<br />
Green-white screened plates<br />
<br />
Picked 4 white colonies from each of gfpt1/2 plates <br />
<br />
Made 5mL LB amp cultures of each colony<br />
<br />
Delivered GFPT1/2 dna samples to biodesign for sequencing (samples from 8/13/12)<br />
<br />
8/15/12<br />
<br />
Miniprepped 3mL of each liquid culture of GFPT1/2<br />
<br />
Prepared glycerol stocks using 100uL of each liquid culture<br />
<br />
Nanodropped samples:<br />
<br />
GFPT1-1 - 172.6 ng/uL<br />
GFPT1-2 - 203.7 ng/uL<br />
GFPT1-3 - 197.4 ng/uL<br />
GFPT1-4 - 178.9 ng/uL<br />
GFPT2-1 - 107.3 ng/uL<br />
GFPT2-2 - 131.2 ng/uL<br />
GFPT2-3 - 145.5 ng/uL<br />
GFPT2-4 - 172.0 ng/uL<br />
<br />
8/17/12 <br />
<br />
GFPT1 sequence confirmed<br />
<br />
Prepared aliquots of GFPT2 minipreps from 8/15/12 for sequencing<br />
<br />
Delivered GFPT2 samples to biodesign for sequencing<br />
<br />
8/19/12 <br />
<br />
GFPT2 sequences confirmed<br />
<br />
<br />
8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
8/29/12<br />
<br />
Discarded GFPT1/2 cultures from 8/27/12<br />
<br />
Revived GFPT1 (from 8/9/12) and GFPT2 (2-2 from 8/15/12) cultures from glycerol scrapes<br />
<br />
Made 4mL cultures in LB Amp<br />
<br />
Digested GFPT1/2 with X and S<br />
<br />
Ran a 1% agarose gel with GFPT1/2 digestions <br />
<br />
Cut out inserts and GFPT2 backbone and stored in 4C for gel extraction and tandum repeat assembly experiments<br />
<br />
8/30/12</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-01T08:00:34Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12<br />
<br />
Picked the colonies again and started new liquid cultures (5mL LB amp).<br />
<br />
Discarded cultures for 8/7/12<br />
<br />
8/9/12<br />
<br />
Miniprepped 3mL of each 8/8/12 culture and nanodropped:<br />
<br />
gfpt1 - 155 ng/uL<br />
<br />
gfpt2 - 114 ng/uL<br />
<br />
Digested gfpt1 and gfpt2 with X and P<br />
<br />
Ran on a 1% agarose gel with the digested GFP plasmid<br />
<br />
Made glyercol stocks with aliquot of the remaining liquid cultures<br />
<br />
<br />
8/13/12<br />
<br />
Prepared sequencing samples</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-01T07:44:27Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
Digested BBa_I13522 with XbaI and PstI.<br />
<br />
Attempted ligating annealed oligos into a digested ?kill plasmid? from Ryan (realized it was cut with E and P).<br />
<br />
8/6/12 <br />
<br />
Annealed oligos for GFPT1 and GFPT2 (target probes)<br />
<br />
Ligated oligos with digested GFP plasmid (BBa_I13522)<br />
<br />
Transformed into competent DH5alpha<br />
<br />
Added SOC and incubated at 37C for 15 minutes.<br />
<br />
Plated on amp treated plates.<br />
<br />
<br />
8/7/12<br />
<br />
Only one colony on each plate (both were white)<br />
<br />
Picked colonies and started 5mL LB amp cultures of each, stored at 37C<br />
<br />
Stored plates in 37C<br />
<br />
8/8/12</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-01T07:23:33Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
<br />
BBa_I13522</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Notebook/hyder
Team:Arizona State/Notebook/hyder
2012-10-01T02:30:57Z
<p>Hyder: Created page with "{{:Team:Arizona_State/Template:Header}} Hyder's lab notes 6/22/12 miniprepped gfp1 and rbs1 and rbs2 liquid cultures picked 1 colony from double terminator (dt1) plate pick..."</p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
Hyder's lab notes<br />
<br />
6/22/12 <br />
<br />
miniprepped gfp1 and rbs1 and rbs2 liquid cultures<br />
<br />
picked 1 colony from double terminator (dt1) plate <br />
<br />
picked 1 colony from t7 polymerase (pol1) plate<br />
<br />
picked 1 colony from puc19 plate (positive control)<br />
<br />
picked 1 colony from dh5a plate (negative control)<br />
<br />
started liquid cultures of each colony (5 mL LB amp each)<br />
<br />
<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O. <br />
<br />
Final Concentration 100uM<br />
<br />
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)<br />
<br />
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)<br />
<br />
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.<br />
<br />
<br />
<br />
8/3/12</div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-09-30T02:32:05Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</td><br />
<td><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<p align="center"><img src="https://mail-attachment.googleusercontent.com/attachment/?ui=2&ik=7aff94f915&view=att&th=13a14dae8fa0639b&attid=0.1&disp=inline&realattid=f_h7pgtg4w0&safe=1&zw&saduie=AG9B_P-GqKscYNzYvEWqbmOmN--v&sadet=1348970411542&sads=Ks19Of60gpXpyLvzLoOQItDskRY" style="width:550px;" /></p><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="175" height="80" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Overview">Overview</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin"><s>Magainin</s></a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">Chimeric Reporter</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA"><s>ssDNA</s></a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Judging_Criteria">Judging Criteria</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Field Applications</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Arizona Community</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">Our University</a> </li> <br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">Parts Submitted to the Registry</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-09-30T02:13:38Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</td><br />
<td><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /></p><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="175" height="80" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Overview">Overview</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin"><s>Magainin</s></a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">Chimeric Reporter</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA"><s>ssDNA</s></a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Judging_Criteria">Judging Criteria</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Field Applications</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Arizona Community</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">Our University</a> </li> <br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">Parts Submitted to the Registry</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-09-30T02:12:41Z
<p>Hyder: </p>
<hr />
<div><html lang="en"><br />
<!-- Made by Abhi & Jordan with help from the "https://2011.igem.org/Team:Imperial_College_London" page --><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<style type="text/css"><br />
#top-section {<br />
width: 975px;<br />
height: 20px;<br />
background-color: transparent;<br />
border: none;<br />
}<br />
<br />
#p-logo { display: none; }<br />
#search-controls { display: none; }<br />
.firstHeading { display: none; }<br />
#contentSub { margin: 0 0 0 0; }<br />
iframe { padding: 10px 20px 10px 20px; }<br />
<br />
body {<br />
background-color:#000000;<br />
background-image:url(https://static.igem.org/mediawiki/2012/c/c8/BackgroundNew.jpg); <br />
background-size:100%;<br />
background-position:center; background-attachment:fixed;<br />
}<br />
<br />
.right-menu li a, .right-menu li a:hover {<br />
color: #3c6b27;<br />
background-color: transparent;<br />
}<br />
<br />
#iGEMLogo {<br />
position: absolute;<br />
top:40px;<br />
left:20px;<br />
}<br />
<br />
#ProjectTitle {<br />
position: relative;<br />
text-align:center;<br />
}<br />
<br />
#ASULogo {<br />
position: absolute;<br />
top:45px;<br />
right:25px;<br />
}<br />
<br />
#menucontainer {<br />
overflow:visible;<br />
position:relative;<br />
z-index:3;<br />
}<br />
<br />
#content {<br />
position: relative;<br />
width: 975px;<br />
margin: 0 auto;<br />
padding-top:60px;<br />
padding-left:0px;<br />
padding-right:0px;<br />
padding-bottom:0px;<br />
//background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/4/4b/2012ASUiGemLogo.png);<br />
//background-repeat:no-repeat;<br />
//background-position:center;<br />
//background-attachment:fixed;<br />
color: black;<br />
border: none;<br />
line-height: 1.5em;<br />
z-index: 2;<br />
}<br />
<br />
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {<br />
margin-bottom: 0;<br />
}<br />
<br />
a {color:#t;}<br />
a:link {color:#93B825;}<br />
a:visited {color:#728F1D;}<br />
a:hover {color:#93B825;}<br />
a:active {color:#93B825;}<br />
a[name]:hover {text-decoration:none;} <br />
<br />
a.sitemap:link,a.sitemap:visited {color:#680000;font-decoration:none;}<br />
a.sitemap:hover,a.sitemap:active {color:#680000;font-decoration:underline;}<br />
<br />
h1 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 2.2em;<br />
margin: 0 0 0 0;<br />
padding: 20px 20px 12px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h2 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.7em;<br />
margin: 0 0 0 0;<br />
padding: 18px 20px 7px 20px;<br />
border-bottom: none;<br />
} <br />
<br />
h3 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.4em;<br />
margin: 0 0 0 0;<br />
padding: 16px 20px 2px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
h4 {<br />
font-family: helvetica,sans-serif;<br />
color: #800000;<br />
background: transparent;<br />
font-weight: bold;<br />
font-size: 1.1em;<br />
margin: 0 0 0 0;<br />
padding: 13.5px 20px 1px 20px;<br />
border-bottom: none;<br />
}<br />
<br />
p {<br />
font-family: helvetica,sans-serif;<br />
//color: #ffffff;<br />
background: transparent;<br />
//background-image:url(https://static.igem.org/mediawiki/2012/e/ea/Layer.png);<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
margin: 0 0 0 0;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
table {<br />
background: transparent;<br />
} th {<br />
background-color:maroon;<br />
color:gold;<br />
}<br />
<br />
.border {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
}<br />
<br />
.borderMagnify {<br />
border:1px solid #B2B2B2;<br />
z-index:101;<br />
margin-left:-9px;<br />
margin-right:9px;<br />
}<br />
<br />
.imgbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.vidbox {<br />
margin:20px;<br />
padding:10px;<br />
border:1px solid black;<br />
text-align:center;<br />
}<br />
<br />
.newouterbox {<br />
background-color:#FF944D;<br />
border:1px solid #CCCCCC;<br />
margin:20px;<br />
padding-bottom:0px;<br />
}<br />
<br />
.newinnerbox {<br />
border:1px solid #CCCCCC;<br />
margin:10px 20px 20px 20px;<br />
padding-top:0px;<br />
padding-bottom:13px;<br />
background-color:#ffffff;<br />
}<br />
<br />
.newtext {<br />
text-align:center;<br />
background-color:#FF944D;<br />
color:#000000;<br />
}<br />
<br />
ul.a {<br />
margin: 0 0 0 40px;<br />
list-style-image: none;<br />
list-style-type:disc;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
ol.a {<br />
margin: 0 0 0 30px;<br />
list-style-position:inside;<br />
font-family: helvetica,sans-serif;<br />
color: #000000;<br />
background: #ffffff;<br />
font-weight: normal;<br />
font-size: 1em;<br />
line-height: 1.7em;<br />
text-align: justify;<br />
padding: 5px 20px 0px 20px;<br />
}<br />
<br />
#BackToTop {<br />
position:fixed;<br />
bottom:0;<br />
right:0;<br />
}<br />
<br />
#Sitemap {<br />
position:fixed;<br />
bottom:0;<br />
left:0;<br />
}<br />
<br />
/*** Start of Styling for menu bar ***/<br />
/*** ESSENTIAL STYLES ***/<br />
a.collapseLink {<br />
font-weight:bold;<br />
font-size:1em;<br />
color:#225323;<br />
}<br />
.sf-menu, .sf-menu * {<br />
margin:0;<br />
padding:0;<br />
list-style:none;<br />
}<br />
.sf-menu {<br />
line-height:1.0;<br />
}<br />
.sf-menu ul {<br />
position:absolute;<br />
top:999em;<br />
width:195px; /* left offset of submenus need to match (see below) */<br />
}<br />
.sf-menu ul li {<br />
width:100%;<br />
}<br />
.sf-menu li:hover {<br />
visibility:inherit; /* fixes IE7 'sticky bug' */<br />
}<br />
.sf-menu li {<br />
float:left;<br />
position:relative;<br />
width:195px;<br />
}<br />
.sf-menu a {<br />
display:block;<br />
position:relative;<br />
}<br />
.sf-menu li:hover ul, .sf-menu li.sfHover ul {<br />
left:0;<br />
top:2.5em; /* match top ul list item height */<br />
z-index:99;<br />
}<br />
ul.sf-menu li:hover li ul, ul.sf-menu li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li:hover ul, ul.sf-menu li li.sfHover ul {<br />
left:15.3em; /* match ul width */<br />
top:0;<br />
}<br />
ul.sf-menu li li:hover li ul, ul.sf-menu li li.sfHover li ul {<br />
top:-999em;<br />
}<br />
ul.sf-menu li li li:hover ul, ul.sf-menu li li li.sfHover ul {<br />
left:10em; /* match ul width */<br />
top:0;<br />
}<br />
<br />
/*** DEMO SKIN ***/<br />
.sf-menu {<br />
float:left;<br />
margin-bottom:1em;<br />
}<br />
.sf-menu a {<br />
border-left:1px solid #fff;<br />
border-top:1px solid #826554;<br />
padding:0.37em 1em 0.37em 1em;<br />
text-decoration:none;<br />
font-family:'helveticaneue', sans-serif;<br />
font-size:1.3em;<br />
}<br />
.sf-menu a, .sf-menu a:visited { /* visited pseudo selector so IE6 applies text colour*/<br />
color:#efefef;<br />
}<br />
.sf-menu li, .sf-menu li li, .sf-menu li li li {<br />
background:#990000;<br />
}<br />
.sf-menu li:hover, .sf-menu li.sfHover, .sf-menu a:focus, .sf-menu a:hover, .sf-menu a:active {<br />
background:#b30000;<br />
outline:0;<br />
}<br />
<br />
/*** arrows **/<br />
.sf-menu a.sf-with-ul {<br />
cursor:default; <br />
padding-right:2.25em;<br />
min-width:1px; /* trigger IE7 hasLayout so spans position accurately */<br />
}<br />
.sf-sub-indicator {<br />
position:absolute;<br />
display:block;<br />
right:.75em;<br />
top:1.05em; /* IE6 only */<br />
width:10px;<br />
height:10px;<br />
text-indent:-999em;<br />
overflow:hidden;<br />
background:url('https://static.igem.org/mediawiki/2011/2/2f/ICL_MenuArrow.png') no-repeat -10px -100px;<br />
/* 8-bit indexed alpha png. IE6 gets solid image only */<br />
}<br />
a > .sf-sub-indicator { /* give all except IE6 the correct values */<br />
top:.8em;<br />
background-position:0 -100px; /* use translucent arrow for modern browsers*/<br />
}<br />
/* apply hovers to modern browsers */<br />
a:focus > .sf-sub-indicator,<br />
a:hover > .sf-sub-indicator,<br />
a:active > .sf-sub-indicator,<br />
li:hover > a > .sf-sub-indicator,<br />
li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px -100px; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/* point right for anchors in subs */<br />
.sf-menu ul .sf-sub-indicator { background-position: -10px 0; }<br />
.sf-menu ul a > .sf-sub-indicator { background-position: 0 0; }<br />
/* apply hovers to modern browsers */<br />
.sf-menu ul a:focus > .sf-sub-indicator,<br />
.sf-menu ul a:hover > .sf-sub-indicator,<br />
.sf-menu ul a:active > .sf-sub-indicator,<br />
.sf-menu ul li:hover > a > .sf-sub-indicator,<br />
.sf-menu ul li.sfHover > a > .sf-sub-indicator {<br />
background-position:-10px 0; /* arrow hovers for modern browsers*/<br />
}<br />
<br />
/*** shadows for all but IE6 ***/<br />
.sf-shadow ul {<br />
background:url('https://static.igem.org/mediawiki/2011/9/9f/ICL_Shadow.png') no-repeat bottom right;<br />
padding:0 8px 9px 0;<br />
-moz-border-radius-bottomleft:17px;<br />
-moz-border-radius-topright:17px;<br />
-webkit-border-top-right-radius:17px;<br />
-webkit-border-bottom-left-radius:17px;<br />
}<br />
<br />
.sf-shadow ul.sf-shadow-off {<br />
background:transparent;<br />
}<br />
</style><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/hoverIntent?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/superfishjs?action=raw&ctype=text/js"><br />
</script> <br />
<script type="text/javascript" src="https://2011.igem.org/Team:Imperial_College_London/magnifier?action=raw&ctype=text/js"><br />
/***********************************************<br />
* jQuery Image Magnify- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* This notice MUST stay intact for legal use<br />
* Visit Dynamic Drive at http://www.dynamicdrive.com/ for this script and 100s more<br />
***********************************************/<br />
</script><br />
<br />
<script type="text/javascript"><br />
var $ = jQuery;<br />
jQuery.imageMagnify.zIndexcounter = 1000;<br />
</script><br />
<br />
<script><br />
$(document).ready(function() {<br />
$("sup").click(function () {<br />
if ($(this).html().substr(0,1)=="[")<br />
{<br />
if ($('.technology').length>0)<br />
{<br />
ddaccordion.expandone('technology', $('.technology').length-1)<br />
setTimeout("window.scrollBy(0,50000)",200)<br />
}<br />
else window.scrollBy(0,50000)<br />
}<br />
});<br />
$("sup").mouseover(function () {<br />
if ($(this).html().substr(0,1)=="[") $(this).css('cursor', 'pointer');<br />
});<br />
});<br />
</script><br />
<br />
<script> <br />
$(document).ready(function() { <br />
$('ul.sf-menu').superfish({ <br />
}); <br />
});<br />
</script><br />
</head><br />
<br />
<body><br />
<a name="top"></a><br />
<!-----<br />
<div id='iGEMLogo'><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</div><br />
<br />
<div id='ProjectTitle'><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /><br />
<!---Before: https://static.igem.org/mediawiki/2012/d/db/2012_Project_logo.png---><!----<br />
</a><br />
</div><br />
<br />
<div id='ASULogo'><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="150" height="70" /><br />
</div><br />
-----><br />
<div id='header' align="center"><br />
<table width="950"><br />
<tr><br />
<td><br />
<a href='https://2012.igem.org/Main_Page'><br />
<img src="https://static.igem.org/mediawiki/2012/d/d6/IGEM_official_logo.png" style="width:120px;" /><br />
</a><br />
</td><br />
<td><br />
<a href='https://2012.igem.org/Team:Arizona_State'><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2012/5/5f/CRSYS.png" style="width:550px;" /></p><br />
</a><br />
</td><br />
<td><br />
<img src="http://afmarcom.com/blog/wp-content/uploads/2011/02/2011-02-25-asu.png" width="175" height="80" /><br />
</td><br />
</table><br />
</div><br />
<br />
<br />
<br />
<div id="BackToTop"><br />
<a href="#top"><br />
<img src="https://static.igem.org/mediawiki/2012/2/2d/ArrowColorChanged.png" width="50px" /><br />
</a><br />
</div><br />
<br />
<div id="Sitemap"><br />
<a href='https://2012.igem.org/Team:Arizona_State/Sitemap'><br />
<img src="https://static.igem.org/mediawiki/2012/3/30/SiteMapColorChange.png" width="100px" /><br />
</a><br />
</div><br />
<br />
<div id='menucontainer'><br />
<ul class="sf-menu sf-navbar"><br />
<li><a class="sf-with-ul" href="#">Project<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State">Home</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Problem">Problem</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Overview">Overview</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Magainin"><s>Magainin</s></a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Chimeric_Reporter">Chimeric Reporter</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/ssDNA"><s>ssDNA</s></a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Notebook">Notebook</a><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/References">References</a><br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Team<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Team">Members</a></li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Attributions">Attributions</a></li><br />
<li><a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a></li><br />
</ul><br />
</li><br />
<br />
<li><a class="sf-with-ul" href="#">Results<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Data">Data</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Main_Results">Main Results</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Judging_Criteria">Judging Criteria</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Regional_Jamboree">Regional Jamboree</a> </li><br />
</ul><br />
</li><br />
<li><a class="sf-with-ul" href="#">Human Practices<span class="sf-sub-indicator"> &#187;</span></a> <br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/International">International Outreach</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/FieldApplications">Field Applications</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/HPModeling">Modeling</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Community">Arizona Community</a> </li><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/University">Our University</a> </li> <br />
</ul> <br />
</li> <br />
<br />
<li><a class="sf-with-ul" href="#">Extras<span class="sf-sub-indicator"> &#187;</span></a><br />
<ul><br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Media">Media</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Parts">Parts Submitted to the Registry</a> </li> <br />
<li><a href="https://2012.igem.org/Team:Arizona_State/Safety">Safety</a> </li> <br />
</ul><br />
</li> <br />
</ul> <br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/LabNotebook/Hyder
Team:Arizona State/LabNotebook/Hyder
2012-09-26T22:42:04Z
<p>Hyder: Created page with "{{:Team:Arizona_State/Template:Header}} Hyder's Lab Notes 6/22/12 Miniprepped GFP1, RBS1, and RBS2 liquid cultures -Eluted wiht 50uL EB buffer Picked one colony from dt1, pol..."</p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<br />
Hyder's Lab Notes<br />
<br />
6/22/12<br />
<br />
Miniprepped GFP1, RBS1, and RBS2 liquid cultures<br />
-Eluted wiht 50uL EB buffer<br />
<br />
Picked one colony from dt1, pol1, PUC19, and negative control DH5a<br />
-Made 5mL liquid cultures in LB Amp for each colony.<br />
<br />
8/3/12<br />
<br />
Resuspended GFPT1 and GFPT2 oligos with Nuclease-Free H2O.<br />
(GFPT1 top1, GFPT1 top2, GFPT1 bot1, GFPT1 bot2, GFPT2 top1, GFPT2 top2, GFPT2 bot1, GFPT2 bot2)<br />
-Final Concentration 100uM<br />
<br />
Preheated heating block to 100C<br />
<br />
Annealed oligos<br />
- 3uL Top1<br />
- 3uL Top2<br />
- 3uL Bot1<br />
- 3uL Bot2<br />
- 2uL Annealing buffer 10x<br />
- 6uL Nuclease-Free H2O<br />
______________________<br />
Final Volume 20uL<br />
<br />
Heated mixtures for 5 minutes at 100C<br />
Let cool to room temp on the metal block <br />
Stored at -20C</div>
Hyder
http://2012.igem.org/Team:Arizona_State
Team:Arizona State
2012-06-25T19:04:59Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<br><br />
<br><br />
<h3>Arizona State University iGEM 2012</h3><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State
Team:Arizona State
2012-06-25T19:04:45Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<html><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<h3>Arizona State University iGEM 2012</h3><br />
<br />
ISN'T OUR NEW WIKI AWESOME?!<br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-06-25T19:03:32Z
<p>Hyder: Undo revision 17842 by Hyder (talk)</p>
<hr />
<div><html><br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script><br />
<script type="text/javascript"><br />
$(document).ready(function(){<br />
<br />
$(function(){<br />
var path = location.pathname.substring(1);<br />
var last = path.split("/");<br />
if ( last[1] ) {<br />
$('#headover ul li a[href*="' + last[1] + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
else {<br />
$('#headover ul li a[href$="' + path + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
});<br />
<br />
$('#menuback').mouseover(function() {<br />
$('#headover').stop().animate({height:'150px',top:'0px'},1000);<br />
});<br />
<br />
$('#menu').mouseleave(function() {<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
$('#headover ul').fadeOut(500);<br />
$('#headover').stop().animate({height:'50px',top:'100px'},1000);<br />
});<br />
<br />
$('.listhead').mouseover(function(){<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$(this).css("color","#444444");<br />
$('#headover ul').css("display","none");<br />
var vActive = $(this).attr("id");<br />
$('.'+vActive).stop(true, true).fadeIn(500);<br />
});<br />
<br />
$('.listmain').mouseover(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
// inner text hover color<br />
$(this).css("color","#1296B0");<br />
});<br />
<br />
$('#headover').mouseleave(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
});<br />
<br />
});<br />
</script><br />
<br />
<style type="text/css"><br />
.firstHeading {<br />
display: none;}<br />
<br />
body {<br />
background-color: #000000;}<br />
#content {<br />
width: 900px;}<br />
<br />
#top-section {<br />
width: 900px;<br />
height: 0;<br />
border-left: none;<br />
border-right: none;<br />
border-bottom: none;<br />
}<br />
<br />
#p-logo {<br />
display:none;<br />
}<br />
<br />
#search-controls {<br />
display: none;}<br />
#mblink a {<br />
text-decoration:none;}<br />
<br />
/* absolute positioning of everythingness */<br />
#menu {<br />
width:900px;<br />
height:150px;<br />
position: relative;<br />
top: 20px;<br />
left: 0px;}<br />
/*left bar color and things */<br />
#menuback {<br />
width:100px;<br />
height:150px;<br />
position: absolute;<br />
top: 0px;<br />
left: 0px;<br />
background-color: #1296B0}<br />
/* right side background */<br />
#headpic {<br />
background-color: black;<br />
width:800px;<br />
height:150px;<br />
position: absolute;<br />
background-repeat:no-repeat;<br />
top: 0px;<br />
left: 100px;<br />
background-image: url('http://a4.ec-images.myspacecdn.com/images02/152/7e16c602354740d78a012c9766c1cadd/l.jpg'); }<br />
<br />
/* positioning of bottom informationy bar thingy */<br />
#headover {<br />
width: 800px;<br />
height:50px;<br />
position: absolute;<br />
top: 100px;<br />
left: 0px;<br />
background: url(https://static.igem.org/mediawiki/2010/d/d8/Imperialigemheadoverone.png) 0px 0px no-repeat; }<br />
<br />
a {<br />
outline:none;}<br />
/* relative positioning of bottom informationy bar thingy */<br />
#headboob{<br />
font-family: helvetica, arial, sans-serif;<br />
font-size: 1.2em;<br />
color: #ffffff;<br />
position: absolute;<br />
top: 0.5em;<br />
right: 1.4em;}<br />
<br />
.dark{<br />
color: #ffffff;}<br />
<br />
.highlight{<br />
color: #1296B0;}<br />
<br />
#menulist{<br />
position: absolute;<br />
top: 16px;<br />
left: 3px;<br />
list-style: none;}<br />
<br />
#menulist li{<br />
height: 30px;<br />
}<br />
<br />
/* outer links */<br />
#menulist li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica;<br />
color: #ffffff;}<br />
<br />
/* bullet points for inner links */<br />
#headover ul{<br />
list-style: none;<br />
display: none;<br />
position: absolute;<br />
top: 1.2em;<br />
left: 0.2em;<br />
}<br />
<br />
/* relative positioning of inner links */<br />
#headover ul ul{<br />
position: absolute;<br />
width:10em;<br />
top:-0.2em;<br />
left: 10em;<br />
list-style:none;}<br />
<br />
/* font of inner links */<br />
#headover ul li{<br />
height: 2.4em;<br />
}<br />
/* font of inner links */<br />
#headover ul li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica, arial, sans-serif;<br />
color: #dddddd;}<br />
<br />
</style><br />
<br />
<br />
<body><br />
<div id='menu'><br />
<div id='menuback'><br />
<ul id='menulist'><br />
<li><a href='#' class='listhead' id='project'>Toast</a></li><br />
<li><a href='#' class='listhead' id='plan'>Toast</a></li><br />
<li><a href='#' class='listhead' id='results'>Toast</a></li><br />
<li><a href='#' class='listhead' id='extra'>Toast</a></li><br />
</ul><br />
</div><br />
<div id='headpic'><br />
<div id='headover'><br />
<p id='headboob'>toast<span class='dark'> &nbsp;<span class='highlight'>|</span>&nbsp;&nbsp;toast detection with a rapid response</span></p><br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Tour/Page_One' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Modules' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Chassis' class='listmain' id='results'>Toast</a></li> <br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Practices' class='listmain' id='results'>Toast Practices</a></li> <br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li> <br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast_One' class='listmain' id='project'>Toast</a></li><br />
</ul><br />
</ul><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Strategy' class='listmain' id='results'>Toast</a></li><br />
<li><a href='http://Toast.igem.org/Team:Toast_College_London/Lab_Diaries' class='listmain' id='Toast'>Toast Diaries</a></li><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Protocol' class='listmain' id='results'>Toast Protocols</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
</ul><br />
<ul class='plan'><br />
</ul><br />
</ul><br />
<ul class='results'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast Results</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<br />
</ul> <br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/The_Team' class='listmain' id='Toast'>The Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Workshops' class='listmain' id='results'>Toast Workshops</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Tool' class='listmain' id='results'>Toast Tool</a></li><br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
</ul><br />
</ul> <br />
</div><br />
</div><br />
</div><br />
</body><br />
<br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-06-25T19:02:53Z
<p>Hyder: </p>
<hr />
<div><html><br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script><br />
<script type="text/javascript"><br />
$(document).ready(function(){<br />
<br />
$(function(){<br />
var path = location.pathname.substring(1);<br />
var last = path.split("/");<br />
if ( last[1] ) {<br />
$('#headover ul li a[href*="' + last[1] + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
else {<br />
$('#headover ul li a[href$="' + path + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
});<br />
<br />
$('#menuback').mouseover(function() {<br />
$('#headover').stop().animate({height:'150px',top:'0px'},1000);<br />
});<br />
<br />
$('#menu').mouseleave(function() {<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
$('#headover ul').fadeOut(500);<br />
$('#headover').stop().animate({height:'50px',top:'100px'},1000);<br />
});<br />
<br />
$('.listhead').mouseover(function(){<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$(this).css("color","#444444");<br />
$('#headover ul').css("display","none");<br />
var vActive = $(this).attr("id");<br />
$('.'+vActive).stop(true, true).fadeIn(500);<br />
});<br />
<br />
$('.listmain').mouseover(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
// inner text hover color<br />
$(this).css("color","#1296B0");<br />
});<br />
<br />
$('#headover').mouseleave(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
});<br />
<br />
});<br />
</script><br />
<br />
<style type="text/css"><br />
.firstHeading {<br />
display: none;}<br />
<br />
body {<br />
background-color: #000000;}<br />
#content {<br />
width: 900px;}<br />
<br />
#top-section {<br />
width: 900px;<br />
height: 0;<br />
border-left: none;<br />
border-right: none;<br />
border-bottom: none;<br />
}<br />
<br />
#p-logo {<br />
display:none;<br />
}<br />
<br />
#search-controls {<br />
display: none;}<br />
#mblink a {<br />
text-decoration:none;}<br />
<br />
/* absolute positioning of everythingness */<br />
#menu {<br />
width:900px;<br />
height:150px;<br />
position: relative;<br />
top: 20px;<br />
left: 0px;}<br />
/*left bar color and things */<br />
#menuback {<br />
width:100px;<br />
height:150px;<br />
position: absolute;<br />
top: 0px;<br />
left: 0px;<br />
background-color: #1296B0}<br />
/* right side background */<br />
#headpic {<br />
background-color: black;<br />
width:800px;<br />
height:150px;<br />
position: absolute;<br />
background-repeat:no-repeat;<br />
top: 0px;<br />
left: 100px;<br />
background-image: url('http://a4.ec-images.myspacecdn.com/images02/152/7e16c602354740d78a012c9766c1cadd/l.jpg'); }<br />
<br />
/* positioning of bottom informationy bar thingy */<br />
#headover {<br />
width: 800px;<br />
height:50px;<br />
position: absolute;<br />
top: 100px;<br />
left: 0px;<br />
background: url(https://static.igem.org/mediawiki/2010/d/d8/Imperialigemheadoverone.png) 0px 0px no-repeat; }<br />
<br />
a {<br />
outline:none;}<br />
/* relative positioning of bottom informationy bar thingy */<br />
#headboob{<br />
font-family: helvetica, arial, sans-serif;<br />
font-size: 1.2em;<br />
color: #ffffff;<br />
position: absolute;<br />
top: 0.5em;<br />
right: 1.4em;}<br />
<br />
.dark{<br />
color: #ffffff;}<br />
<br />
.highlight{<br />
color: #1296B0;}<br />
<br />
#menulist{<br />
position: absolute;<br />
top: 16px;<br />
left: 3px;<br />
list-style: none;}<br />
<br />
#menulist li{<br />
height: 30px;<br />
}<br />
<br />
/* outer links */<br />
#menulist li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica;<br />
color: #ffffff;}<br />
<br />
/* bullet points for inner links */<br />
#headover ul{<br />
list-style: none;<br />
display: none;<br />
position: absolute;<br />
top: 1.2em;<br />
left: 0.2em;<br />
}<br />
<br />
/* relative positioning of inner links */<br />
#headover ul ul{<br />
position: absolute;<br />
width:10em;<br />
top:-0.2em;<br />
left: 10em;<br />
list-style:none;}<br />
<br />
/* font of inner links */<br />
#headover ul li{<br />
height: 2.4em;<br />
}<br />
/* font of inner links */<br />
#headover ul li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica, arial, sans-serif;<br />
color: #dddddd;}<br />
<br />
</style><br />
<br />
<br />
<body><br />
<div id='menu'><br />
<div id='menuback'><br />
<ul id='menulist'><br />
<li><a href='#' class='listhead' id='project'></a></li><br />
<li><a href='#' class='listhead' id='plan'></a></li><br />
<li><a href='#' class='listhead' id='results'></a></li><br />
<li><a href='#' class='listhead' id='extra'></a></li><br />
</ul><br />
</div><br />
<div id='headpic'><br />
<div id='headover'><br />
<p id='headboob'><span class='dark'> &nbsp;<span class='highlight'>|</span>&nbsp;&nbsp; detection with a rapid response</span></p><br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:_College_London' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/Tour/Page_One' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/Modules' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/Chassis' class='listmain' id='results'></a></li> <br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:_College_London/_Practices' class='listmain' id='results'> Practices</a></li> <br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li> <br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London//_One' class='listmain' id='project'></a></li><br />
</ul><br />
</ul><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/Strategy' class='listmain' id='results'></a></li><br />
<li><a href='http://.igem.org/Team:_College_London/Lab_Diaries' class='listmain' id=''> Diaries</a></li><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:_College_London/Protocol' class='listmain' id='results'> Protocols</a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
</ul><br />
<ul class='plan'><br />
</ul><br />
</ul><br />
<ul class='results'><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'> Results</a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London//' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
<br />
</ul> <br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:_College_London/The_Team' class='listmain' id=''>The </a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London//' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/_Workshops' class='listmain' id='results'> Workshops</a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/_Tool' class='listmain' id='results'> Tool</a></li><br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
<li><a href='https://2010.igem.org/Team:_College_London/' class='listmain' id='results'></a></li><br />
</ul><br />
</ul> <br />
</div><br />
</div><br />
</div><br />
</body><br />
<br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State
Team:Arizona State
2012-06-25T19:02:07Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona_State/Template:Header}}<br />
<br />
<html><br />
<h3> Arizona State University iGEM 2012 </h3><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-06-25T19:01:44Z
<p>Hyder: </p>
<hr />
<div><html><br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script><br />
<script type="text/javascript"><br />
$(document).ready(function(){<br />
<br />
$(function(){<br />
var path = location.pathname.substring(1);<br />
var last = path.split("/");<br />
if ( last[1] ) {<br />
$('#headover ul li a[href*="' + last[1] + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
else {<br />
$('#headover ul li a[href$="' + path + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
});<br />
<br />
$('#menuback').mouseover(function() {<br />
$('#headover').stop().animate({height:'150px',top:'0px'},1000);<br />
});<br />
<br />
$('#menu').mouseleave(function() {<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
$('#headover ul').fadeOut(500);<br />
$('#headover').stop().animate({height:'50px',top:'100px'},1000);<br />
});<br />
<br />
$('.listhead').mouseover(function(){<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$(this).css("color","#444444");<br />
$('#headover ul').css("display","none");<br />
var vActive = $(this).attr("id");<br />
$('.'+vActive).stop(true, true).fadeIn(500);<br />
});<br />
<br />
$('.listmain').mouseover(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
// inner text hover color<br />
$(this).css("color","#1296B0");<br />
});<br />
<br />
$('#headover').mouseleave(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
});<br />
<br />
});<br />
</script><br />
<br />
<style type="text/css"><br />
.firstHeading {<br />
display: none;}<br />
<br />
body {<br />
background-color: #000000;}<br />
#content {<br />
width: 900px;}<br />
<br />
#top-section {<br />
width: 900px;<br />
height: 0;<br />
border-left: none;<br />
border-right: none;<br />
border-bottom: none;<br />
}<br />
<br />
#p-logo {<br />
display:none;<br />
}<br />
<br />
#search-controls {<br />
display: none;}<br />
#mblink a {<br />
text-decoration:none;}<br />
<br />
/* absolute positioning of everythingness */<br />
#menu {<br />
width:900px;<br />
height:150px;<br />
position: relative;<br />
top: 20px;<br />
left: 0px;}<br />
/*left bar color and things */<br />
#menuback {<br />
width:100px;<br />
height:150px;<br />
position: absolute;<br />
top: 0px;<br />
left: 0px;<br />
background-color: #1296B0}<br />
/* right side background */<br />
#headpic {<br />
background-color: black;<br />
width:800px;<br />
height:150px;<br />
position: absolute;<br />
background-repeat:no-repeat;<br />
top: 0px;<br />
left: 100px;<br />
background-image: url('http://a4.ec-images.myspacecdn.com/images02/152/7e16c602354740d78a012c9766c1cadd/l.jpg'); }<br />
<br />
/* positioning of bottom informationy bar thingy */<br />
#headover {<br />
width: 800px;<br />
height:50px;<br />
position: absolute;<br />
top: 100px;<br />
left: 0px;<br />
background: url(https://static.igem.org/mediawiki/2010/d/d8/Imperialigemheadoverone.png) 0px 0px no-repeat; }<br />
<br />
a {<br />
outline:none;}<br />
/* relative positioning of bottom informationy bar thingy */<br />
#headboob{<br />
font-family: helvetica, arial, sans-serif;<br />
font-size: 1.2em;<br />
color: #ffffff;<br />
position: absolute;<br />
top: 0.5em;<br />
right: 1.4em;}<br />
<br />
.dark{<br />
color: #ffffff;}<br />
<br />
.highlight{<br />
color: #1296B0;}<br />
<br />
#menulist{<br />
position: absolute;<br />
top: 16px;<br />
left: 3px;<br />
list-style: none;}<br />
<br />
#menulist li{<br />
height: 30px;<br />
}<br />
<br />
/* outer links */<br />
#menulist li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica;<br />
color: #ffffff;}<br />
<br />
/* bullet points for inner links */<br />
#headover ul{<br />
list-style: none;<br />
display: none;<br />
position: absolute;<br />
top: 1.2em;<br />
left: 0.2em;<br />
}<br />
<br />
/* relative positioning of inner links */<br />
#headover ul ul{<br />
position: absolute;<br />
width:10em;<br />
top:-0.2em;<br />
left: 10em;<br />
list-style:none;}<br />
<br />
/* font of inner links */<br />
#headover ul li{<br />
height: 2.4em;<br />
}<br />
/* font of inner links */<br />
#headover ul li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica, arial, sans-serif;<br />
color: #dddddd;}<br />
<br />
</style><br />
<br />
<br />
<body><br />
<div id='menu'><br />
<div id='menuback'><br />
<ul id='menulist'><br />
<li><a href='#' class='listhead' id='project'>Toast</a></li><br />
<li><a href='#' class='listhead' id='plan'>Toast</a></li><br />
<li><a href='#' class='listhead' id='results'>Toast</a></li><br />
<li><a href='#' class='listhead' id='extra'>Toast</a></li><br />
</ul><br />
</div><br />
<div id='headpic'><br />
<div id='headover'><br />
<p id='headboob'>toast<span class='dark'> &nbsp;<span class='highlight'>|</span>&nbsp;&nbsp;toast detection with a rapid response</span></p><br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Tour/Page_One' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Modules' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Chassis' class='listmain' id='results'>Toast</a></li> <br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Practices' class='listmain' id='results'>Toast Practices</a></li> <br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li> <br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast_One' class='listmain' id='project'>Toast</a></li><br />
</ul><br />
</ul><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Strategy' class='listmain' id='results'>Toast</a></li><br />
<li><a href='http://Toast.igem.org/Team:Toast_College_London/Lab_Diaries' class='listmain' id='Toast'>Toast Diaries</a></li><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Protocol' class='listmain' id='results'>Toast Protocols</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
</ul><br />
<ul class='plan'><br />
</ul><br />
</ul><br />
<ul class='results'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast Results</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<br />
</ul> <br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/The_Team' class='listmain' id='Toast'>The Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Workshops' class='listmain' id='results'>Toast Workshops</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Tool' class='listmain' id='results'>Toast Tool</a></li><br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
</ul><br />
</ul> <br />
</div><br />
</div><br />
</div><br />
</body><br />
<br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-06-25T18:46:42Z
<p>Hyder: </p>
<hr />
<div><html><br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script><br />
<script type="text/javascript"><br />
$(document).ready(function(){<br />
<br />
$(function(){<br />
var path = location.pathname.substring(1);<br />
var last = path.split("/");<br />
if ( last[1] ) {<br />
$('#headover ul li a[href*="' + last[1] + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
else {<br />
$('#headover ul li a[href$="' + path + '"]').addClass("curlink").css("color","#ffffff");<br />
}<br />
});<br />
<br />
$('#menuback').mouseover(function() {<br />
$('#headover').stop().animate({height:'150px',top:'0px'},1000);<br />
});<br />
<br />
$('#menu').mouseleave(function() {<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
$('#headover ul').fadeOut(500);<br />
$('#headover').stop().animate({height:'50px',top:'100px'},1000);<br />
});<br />
<br />
$('.listhead').mouseover(function(){<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$(this).css("color","#444444");<br />
$('#headover ul').css("display","none");<br />
var vActive = $(this).attr("id");<br />
$('.'+vActive).stop(true, true).fadeIn(500);<br />
});<br />
<br />
$('.listmain').mouseover(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
// inner text hover color<br />
$(this).css("color","#1296B0");<br />
});<br />
<br />
$('#headover').mouseleave(function(){<br />
$('.listmain').not('.curlink').css("color","#ffffff");<br />
});<br />
<br />
});<br />
</script><br />
<br />
<style type="text/css"><br />
.firstHeading {<br />
display: none;}<br />
<br />
body {<br />
background-color: #000000;}<br />
#content {<br />
width: 900px;}<br />
<br />
#top-section {<br />
display: none;}<br />
<br />
#search-controls {<br />
display: none;}<br />
#mblink a {<br />
text-decoration:none;}<br />
<br />
/* absolute positioning of everythingness */<br />
#menu {<br />
width:900px;<br />
height:150px;<br />
position: relative;<br />
top: 20px;<br />
left: 0px;}<br />
/*left bar color and things */<br />
#menuback {<br />
width:100px;<br />
height:150px;<br />
position: absolute;<br />
top: 0px;<br />
left: 0px;<br />
background-color: #1296B0}<br />
/* right side background */<br />
#headpic {<br />
background-color: black;<br />
width:800px;<br />
height:150px;<br />
position: absolute;<br />
background-repeat:no-repeat;<br />
top: 0px;<br />
left: 100px;<br />
background-image: url('http://a4.ec-images.myspacecdn.com/images02/152/7e16c602354740d78a012c9766c1cadd/l.jpg'); }<br />
<br />
/* positioning of bottom informationy bar thingy */<br />
#headover {<br />
width: 800px;<br />
height:50px;<br />
position: absolute;<br />
top: 100px;<br />
left: 0px;<br />
background: url(https://static.igem.org/mediawiki/2010/d/d8/Imperialigemheadoverone.png) 0px 0px no-repeat; }<br />
<br />
a {<br />
outline:none;}<br />
/* relative positioning of bottom informationy bar thingy */<br />
#headboob{<br />
font-family: helvetica, arial, sans-serif;<br />
font-size: 1.2em;<br />
color: #ffffff;<br />
position: absolute;<br />
top: 0.5em;<br />
right: 1.4em;}<br />
<br />
.dark{<br />
color: #ffffff;}<br />
<br />
.highlight{<br />
color: #1296B0;}<br />
<br />
#menulist{<br />
position: absolute;<br />
top: 16px;<br />
left: 3px;<br />
list-style: none;}<br />
<br />
#menulist li{<br />
height: 30px;<br />
}<br />
<br />
/* outer links */<br />
#menulist li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica;<br />
color: #ffffff;}<br />
<br />
/* bullet points for inner links */<br />
#headover ul{<br />
list-style: none;<br />
display: none;<br />
position: absolute;<br />
top: 1.2em;<br />
left: 0.2em;<br />
}<br />
<br />
/* relative positioning of inner links */<br />
#headover ul ul{<br />
position: absolute;<br />
width:10em;<br />
top:-0.2em;<br />
left: 10em;<br />
list-style:none;}<br />
<br />
/* font of inner links */<br />
#headover ul li{<br />
height: 2.4em;<br />
}<br />
/* font of inner links */<br />
#headover ul li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica, arial, sans-serif;<br />
color: #dddddd;}<br />
<br />
</style><br />
<br />
<br />
<body><br />
<div id='menu'><br />
<div id='menuback'><br />
<ul id='menulist'><br />
<li><a href='#' class='listhead' id='project'>Toast</a></li><br />
<li><a href='#' class='listhead' id='plan'>Toast</a></li><br />
<li><a href='#' class='listhead' id='results'>Toast</a></li><br />
<li><a href='#' class='listhead' id='extra'>Toast</a></li><br />
</ul><br />
</div><br />
<div id='headpic'><br />
<div id='headover'><br />
<p id='headboob'>toast<span class='dark'> &nbsp;<span class='highlight'>|</span>&nbsp;&nbsp;toast detection with a rapid response</span></p><br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Tour/Page_One' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Modules' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Chassis' class='listmain' id='results'>Toast</a></li> <br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Practices' class='listmain' id='results'>Toast Practices</a></li> <br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li> <br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast_One' class='listmain' id='project'>Toast</a></li><br />
</ul><br />
</ul><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Strategy' class='listmain' id='results'>Toast</a></li><br />
<li><a href='http://Toast.igem.org/Team:Toast_College_London/Lab_Diaries' class='listmain' id='Toast'>Toast Diaries</a></li><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Protocol' class='listmain' id='results'>Toast Protocols</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
</ul><br />
<ul class='plan'><br />
</ul><br />
</ul><br />
<ul class='results'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast Results</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<br />
</ul> <br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/The_Team' class='listmain' id='Toast'>The Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Workshops' class='listmain' id='results'>Toast Workshops</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast_Tool' class='listmain' id='results'>Toast Tool</a></li><br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
<li><a href='https://2010.igem.org/Team:Toast_College_London/Toast' class='listmain' id='results'>Toast</a></li><br />
</ul><br />
</ul> <br />
</div><br />
</div><br />
</div><br />
</body><br />
<br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-06-25T17:14:51Z
<p>Hyder: Blanked the page</p>
<hr />
<div></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Template:Header
Team:Arizona State/Template:Header
2012-06-25T17:14:16Z
<p>Hyder: </p>
<hr />
<div><html><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script><br />
<script type="text/javascript"><br />
$(document).ready(function(){<br />
<br />
$(function(){<br />
var path = location.pathname.substring(1);<br />
var last = path.split("/");<br />
if ( last[1] ) {<br />
$('#headover ul li a[href*="' + last[1] + '"]').addClass("curlink").css("color","#ea8828");<br />
}<br />
else {<br />
$('#headover ul li a[href$="' + path + '"]').addClass("curlink").css("color","#ea8828");<br />
}<br />
});<br />
<br />
$('#menuback').mouseover(function() {<br />
$('#headover').stop().animate({height:'150px',top:'0px'},1000);<br />
});<br />
<br />
$('#menu').mouseleave(function() {<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$('.listmain').not('.curlink').css("color","#dddddd");<br />
$('#headover ul').fadeOut(500);<br />
$('#headover').stop().animate({height:'50px',top:'100px'},1000);<br />
});<br />
<br />
$('.listhead').mouseover(function(){<br />
$('.listhead').not('.curlink').css("color","#ffffff");<br />
$(this).css("color","#444444");<br />
$('#headover ul').css("display","none");<br />
var vActive = $(this).attr("id");<br />
$('.'+vActive).stop(true, true).fadeIn(500);<br />
});<br />
<br />
$('.listmain').mouseover(function(){<br />
$('.listmain').not('.curlink').css("color","#dddddd");<br />
$(this).css("color","#ea8828");<br />
});<br />
<br />
$('#headover').mouseleave(function(){<br />
$('.listmain').not('.curlink').css("color","#dddddd");<br />
});<br />
<br />
});<br />
</script><br />
<style type="text/css"><br />
<br />
a:link {color:#20548f;}<br />
a:visited {color:#20548f;}<br />
a:hover {color:#20548f;}<br />
a:active {color:#20548f;}<br />
<br />
#top-section {<br />
background-color: #ffffff;<br />
height: 100px;<br />
border: none;<br />
width:910px;<br />
height:20px}<br />
<br />
#p-logo {<br />
position: absolute;<br />
display: none;}<br />
<br />
#search-controls {<br />
display: none;}<br />
<br />
#menubar.left-menu {<br />
position: absolute;<br />
top:0px;<br />
left:0px;}<br />
<br />
#menubar.right-menu {<br />
position: absolute;<br />
top:0px;<br />
right:0px;}<br />
<br />
<br />
#menubar a:active,#menubar a:hover,#menubar a:link,#menubar a:visited {<br />
color: #000000;}<br />
<br />
#footer-box {<br />
width:900px;}<br />
<br />
body {<br />
background-color: #555555}<br />
<br />
.firstHeading {<br />
display: none;}<br />
<br />
#content {<br />
width: 900px;<br />
border: none;<br />
font-family: helvetica, arial, sans-serif;<br />
color: #555555}<br />
<br />
#title {<br />
width:900px;<br />
height:55px;<br />
position: relative;<br />
top: 0px;<br />
left: 0px;}<br />
<br />
#paralogo {<br />
width:400px;<br />
height:50px;<br />
position: relative;<br />
top: 0px;<br />
left: 20px;}<br />
<br />
#implogo {<br />
width:200px;<br />
height:50px;<br />
position: absolute;<br />
top: 0px;<br />
left: 435px;}<br />
<br />
#igemlogo {<br />
width:84px;<br />
height:50px;<br />
position:absolute;<br />
top:0px;<br />
left:615px;}<br />
<br />
#csynbilogo {<br />
width:140px;<br />
height:35px;<br />
position:absolute;<br />
top:-10px;<br />
left:695px;}<br />
<br />
#csbl {<br />
width:140px;<br />
height:35px;}<br />
<br />
#belink {<br />
width:200px;<br />
height:16px;<br />
position: absolute;<br />
top: 25px;<br />
font-size:1em;<br />
left: 697px;<br />
font-weight:500;}<br />
<br />
#belink a {<br />
text-decoration:none;}<br />
<br />
#mblink {<br />
width:205px;<br />
height:16px;<br />
position: absolute;<br />
top: 42px;<br />
font-size:1em;<br />
left: 697px;<br />
font-weight:500;}<br />
<br />
#mblink a {<br />
text-decoration:none;}<br />
<br />
#menu {<br />
width:900px;<br />
height:150px;<br />
position: relative;<br />
top: 20px;<br />
left: 0px;}<br />
<br />
#menuback {<br />
width:100px;<br />
height:150px;<br />
position: absolute;<br />
top: 0px;<br />
left: 0px;<br />
background-color: #ea8828}<br />
<br />
#headpic {<br />
width:800px;<br />
height:150px;<br />
position: absolute;<br />
top: 0px;<br />
left: 100px;<br />
background-image: url('https://static.igem.org/mediawiki/2010/5/54/Imperialigemheadone.jpg'); }<br />
<br />
#headover {<br />
width: 800px;<br />
height:50px;<br />
position: absolute;<br />
top: 100px;<br />
left: 0px;<br />
background: url(https://static.igem.org/mediawiki/2010/d/d8/Imperialigemheadoverone.png) 0px 0px no-repeat; }<br />
<br />
a {<br />
outline:none;}<br />
<br />
#headtit{<br />
font-family: helvetica, arial, sans-serif;<br />
font-size: 1.2em;<br />
color: #ffffff;<br />
position: absolute;<br />
top: 0.5em;<br />
right: 1.4em;}<br />
<br />
.dark{<br />
color: #dddddd;}<br />
<br />
.highlight{<br />
color: #ea8828;}<br />
<br />
#menulist{<br />
position: absolute;<br />
top: 16px;<br />
left: 3px;<br />
list-style: none;}<br />
<br />
#menulist li{<br />
height: 30px;<br />
}<br />
<br />
#menulist li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica, arial, sans-serif;<br />
color: #ffffff;}<br />
<br />
#headover ul{<br />
list-style: none;<br />
display: none;<br />
position: absolute;<br />
top: 1.2em;<br />
left: 0.2em;<br />
}<br />
<br />
#headover ul ul{<br />
position: absolute;<br />
width:10em;<br />
top:-0.2em;<br />
left: 10em;<br />
list-style:none;}<br />
<br />
#headover ul li{<br />
height: 2.4em;<br />
}<br />
<br />
#headover ul li a{<br />
font-weight: 100;<br />
font-size: 1.2em;<br />
text-decoration: none;<br />
font-family: helvetica, arial, sans-serif;<br />
color: #dddddd;}<br />
<br />
</style><br />
</head><br />
<body><br />
<div id='title'><br />
<div id='paralogo'><br />
<a href='https://2010.igem.org/Team:Imperial_College_London'><br />
<img src='https://static.igem.org/mediawiki/2010/9/9f/ICparalogo.png'><br />
</a><br />
</div><br />
<div id='implogo'><br />
<a href='http://www3.imperial.ac.uk/'><br />
<img src='https://static.igem.org/mediawiki/2010/8/84/ICLlogo1.png'><br />
</a><br />
</div><br />
<div id='igemlogo'><br />
<a href='https://2010.igem.org/Main_Page'><br />
<img src='https://static.igem.org/mediawiki/2010/7/7d/ICLlogo2.png'><br />
</a><br />
</div><br />
<div id='csynbilogo'><br />
<a href='http://www3.imperial.ac.uk/syntheticbiology'><br />
<img id="csbl" src='https://static.igem.org/mediawiki/2010/7/7f/ICLlogo3.png'><br />
</a><br />
</div><br />
<div id='belink'><br />
<a href='http://www3.imperial.ac.uk/bioengineering'>Department of Bioengineering</a><br />
</div><br />
<div id='mblink'><br />
<a href='http://www3.imperial.ac.uk/molecularbiosciences'>Division of Molecular Biosciences</a><br />
</div><br />
</div><br />
<div id='menu'><br />
<div id='menuback'><br />
<ul id='menulist'><br />
<li><a href='#' class='listhead' id='project'>Project</a></li><br />
<li><a href='#' class='listhead' id='plan'>Plan</a></li><br />
<li><a href='#' class='listhead' id='results'>Results</a></li><br />
<li><a href='#' class='listhead' id='extra'>Extras</a></li><br />
</ul><br />
</div><br />
<div id='headpic'><br />
<div id='headover'><br />
<p id='headtit'>Parasight<span class='dark'> &nbsp;<span class='highlight'>|</span>&nbsp;&nbsp;Parasite detection with a rapid response</span></p><br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London' class='listmain' id='results'>Home</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Tour/Page_One' class='listmain' id='results'>Tour</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Modules' class='listmain' id='results'>Modules</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Chassis' class='listmain' id='results'>Chassis</a></li> <br />
<ul class='project'><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Human_Practices' class='listmain' id='results'>Human Practices</a></li> <br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Schistosoma' class='listmain' id='results'>Schistosoma</a></li> <br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Research' class='listmain' id='results'>Research</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Diary/Week_One' class='listmain' id='project'>Diary</a></li><br />
</ul><br />
</ul><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Modelling' class='listmain' id='results'>Modelling</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Strategy' class='listmain' id='results'>Assembly</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Lab_Diaries' class='listmain' id='results'>Lab Diaries</a></li><br />
<ul class='plan'><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Protocol' class='listmain' id='results'>Lab Protocols</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Safety' class='listmain' id='results'>Safety</a></li><br />
</ul><br />
<ul class='plan'><br />
</ul><br />
</ul><br />
<ul class='results'><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Results' class='listmain' id='results'>Experimental Results</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Parts/Favourites' class='listmain' id='results'>Parts</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Achievements' class='listmain' id='results'>Achievements</a></li><br />
<br />
</ul> <br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/The_Team' class='listmain' id='results'>The Team</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Media/Videos' class='listmain' id='results'>Media</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/School_Workshops' class='listmain' id='results'>School Workshops</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Software_Tool' class='listmain' id='results'>Software Tool</a></li><br />
<ul class='extra'><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Acknowledgments' class='listmain' id='results'>Acknowledgments</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Brainstorming' class='listmain' id='results'>Brainstorming</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Glossary' class='listmain' id='results'>Glossary</a></li><br />
<li><a href='https://2010.igem.org/Team:Imperial_College_London/Sitemap' class='listmain' id='results'>Sitemap</a></li><br />
</ul><br />
</ul> <br />
</div><br />
</div><br />
</div><br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Templates/main
Team:Arizona State/Templates/main
2012-05-09T05:34:59Z
<p>Hyder: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
body {background: #FFFFFF;}<br />
.firstHeading { display: none; } <br />
#top-section {<br />
background-image: url('https://static.igem.org/mediawiki/2011/6/6d/ASU_banner_black.jpg');<br />
height:200px ! important;;<br />
}<br />
<br />
#search-bar { display: none; }<br />
<br />
#p-logo {<br />
height:80px ! important;;<br />
overflow:hidden;<br />
visibility:hidden;<br />
}<br />
body<br />
{<br />
background-image:url('https://static.igem.org/mediawiki/2011/2/27/ASU_Circos_Ecoli.png');<br />
}<br />
</style><br />
</html><br />
<br><br />
<div style="font-size:18pt;"><br />
<font face="Calibri" style="color:#000000"><center>{{{title}}}<br />
----<br />
</center></font></div><br />
<div style="color: #ffffff; background-color: #ffffff; width: 900px"><br />
</div><br />
<br />
<div><br />
{| cellspacing="0"<br />
|-<br />
|style="background-color: #ffffff" width="75px" valign="top"|<br />
<br>{{:Team:Arizona State/Templates/sidebar2}}<br />
<br />
|width="880" valign="top" style="padding: 10px; border: 5px solid #ffffff; color: #000; background-color: white" | <br />
<div style="height: 450px; border: 1px solid #ffffff;"></div><div style="position: relative; margin-top: -450px; min-height: 450px">[[File:ASU_Logo.png|110px|right|link=Team:Arizona State]]<div>{{{content}}}</div><br />
</div><br />
|}<br />
----<br />
<center>{{:Team:Arizona State/Templates/footer}}</center></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Templates/footer
Team:Arizona State/Templates/footer
2012-05-09T05:23:44Z
<p>Hyder: Created page with "<html> <div id="clear"></div> <div style="color: #000000; background-color: #EBEBEB"> <div id="content-footer-box"> <div class="footer-left-half"> ..."</p>
<hr />
<div><html><br />
<div id="clear"></div><br />
<div style="color: #000000; background-color: #EBEBEB"><br />
<br />
<div id="content-footer-box"><br />
<br />
<div class="footer-left-half"><br />
<div class="footer-left-col"><br />
<br><br />
<small><br />
<p><b>Contact Us</b></p><br />
<p>Arizona State University</p><br />
<p>ECG 334, PO BOX 9709</p><br />
<p>Tempe, Arizona 85287</p><br />
<p><a href="mailto:asu.igem@gmail.com">Email</a></p><br />
</small><br />
<br />
</div><br />
<div class="footer-right-col"><br />
<br />
</div><br />
</div><br />
<div class="footer-right-half"><br />
<div class="footer-right-left-col"><br />
<br />
</div><br />
<div class="footer-right-right-col"><br />
<br />
<br />
<script src="http://widgets.twimg.com/j/2/widget.js"></script><br />
<script><br />
new TWTR.Widget({<br />
version: 2,<br />
type: 'profile',<br />
rpp: 2,<br />
interval: 30000,<br />
width: 250,<br />
height: 300,<br />
theme: {<br />
shell: {<br />
background: '#333333',<br />
color: '#ffffff'<br />
},<br />
tweets: {<br />
background: '#000000',<br />
color: '#ffffff',<br />
links: '#4aed05'<br />
}<br />
},<br />
features: {<br />
scrollbar: false,<br />
loop: false,<br />
live: false,<br />
hashtags: true,<br />
timestamp: true,<br />
avatars: false,<br />
behavior: 'all'<br />
}<br />
}).render().setUser('asu_igem').start();<br />
</script><br />
<br />
<br />
<br />
<br><br />
<br />
</div><br />
</div><br />
<br />
<div id="clear"></div><br />
<br />
</div><br />
</div><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Templates/sidebar2
Team:Arizona State/Templates/sidebar2
2012-05-09T05:23:28Z
<p>Hyder: Created page with "<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en"> <head> <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script> ..."</p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en"><br />
<br />
<head><br />
<br />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script><br />
<br />
<script type="text/javascript" src="https://2011.igem.org/Team:Arizona_State/Templates/accordion?action=raw&ctype=text/javascript"><br />
<br />
/***********************************************<br />
* Accordion Content script- (c) Dynamic Drive DHTML code library (www.dynamicdrive.com)<br />
* Visit http://www.dynamicDrive.com for hundreds of DHTML scripts<br />
* This notice must stay intact for legal use<br />
***********************************************/<br />
<br />
</script><br />
<br />
<br />
<script type="text/javascript"><br />
<br />
<br />
ddaccordion.init({<br />
headerclass: "submenuheader", //Shared CSS class name of headers group<br />
contentclass: "submenu", //Shared CSS class name of contents group<br />
revealtype: "click", //Reveal content when user clicks or onmouseover the header? Valid value: "click", "clickgo", or "mouseover"<br />
mouseoverdelay: 200, //if revealtype="mouseover", set delay in milliseconds before header expands onMouseover<br />
collapseprev: true, //Collapse previous content (so only one open at any time)? true/false <br />
defaultexpanded: [], //index of content(s) open by default [index1, index2, etc] [] denotes no content<br />
onemustopen: false, //Specify whether at least one header should be open always (so never all headers closed)<br />
animatedefault: false, //Should contents open by default be animated into view?<br />
persiststate: true, //persist state of opened contents within browser session?<br />
toggleclass: ["", ""], //Two CSS classes to be applied to the header when it's collapsed and expanded, respectively ["class1", "class2"]<br />
togglehtml: ["suffix", "<img src='https://static.igem.org/mediawiki/2011/9/9c/Arizona_State_plus.gif' class='statusicon' />", "<img src='https://static.igem.org/mediawiki/2011/8/84/Arizona_State_minus.gif' class='statusicon' />"], //Additional HTML added to the header when it's collapsed and expanded, respectively ["position", "html1", "html2"] (see docs)<br />
animatespeed: "fast", //speed of animation: integer in milliseconds (ie: 200), or keywords "fast", "normal", or "slow"<br />
oninit:function(headers, expandedindices){ //custom code to run when headers have initalized<br />
//do nothing<br />
},<br />
onopenclose:function(header, index, state, isuseractivated){ //custom code to run whenever a header is opened or closed<br />
//do nothing<br />
}<br />
})<br />
<br />
<br />
</script><br />
<br />
<br />
<style type="text/css"><br />
<br />
.glossymenu{<br />
margin: 5px 0;<br />
padding: 0;<br />
width: 170px; /*width of menu*/<br />
border: 1px solid #9A9A9A;<br />
border-bottom-width: 0;<br />
}<br />
<br />
.glossymenu a.menuitem{<br />
background: black url(https://static.igem.org/mediawiki/2011/0/0d/Arizona_State_glossyback2.gif) repeat-x bottom left;<br />
font: bold 14px "Verdana", "Trebuchet MS", Verdana, Helvetica, sans-serif;<br />
color: white;<br />
display: block;<br />
position: relative; /*To help in the anchoring of the ".statusicon" icon image*/<br />
width: auto;<br />
padding: 4px 0;<br />
padding-left: 10px;<br />
text-decoration: none;<br />
}<br />
<br />
<br />
.glossymenu a.menuitem:visited, .glossymenu .menuitem:active{<br />
color: white;<br />
}<br />
<br />
.glossymenu a.menuitem .statusicon{ /*CSS for icon image that gets dynamically added to headers*/<br />
position: absolute;<br />
top: 5px;<br />
right: 5px;<br />
border: none;<br />
}<br />
<br />
.glossymenu a.menuitem:hover{<br />
background-image: url(https://static.igem.org/mediawiki/2011/0/0d/Arizona_State_glossyback2.gif);<br />
}<br />
<br />
.glossymenu div.submenu{ /*DIV that contains each sub menu*/<br />
background: white;<br />
}<br />
<br />
.glossymenu div.submenu ul{ /*UL of each sub menu*/<br />
list-style-type: none;<br />
margin: 0;<br />
padding: 0;<br />
}<br />
<br />
.glossymenu div.submenu ul li{<br />
border-bottom: 1px solid black;<br />
}<br />
<br />
.glossymenu div.submenu ul li a{<br />
display: block;<br />
font: normal 13px "Lucida Grande", "Trebuchet MS", Verdana, Helvetica, sans-serif;<br />
color: black;<br />
text-decoration: none;<br />
padding: 2px 0;<br />
padding-left: 10px;<br />
}<br />
<br />
.glossymenu div.submenu ul li a:hover{<br />
background: #DFDCCB;<br />
colorz: white;<br />
}<br />
<br />
</style><br />
<br />
</head><br />
<br />
<body><br />
<br />
<div class="glossymenu"><br />
<a class="menuitem" href="https://2011.igem.org/Team:Arizona_State">Home</a><br />
<a class="menuitem" href="https://2011.igem.org/Main_Page">iGEM 2011 Home</a><br />
<a class="menuitem submenuheader" href="https://2011.igem.org/Team:Arizona_State/Project">Project</a><br />
<div class="submenu"><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/Introduction">Introduction</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/CRISPR">CRISPR</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/E_coli">E. coli</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/B_halodurans">B. halodurans</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/L_innocua">L. innocua</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/Software">Software</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/Future">Future</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Project/References">References</a></li><br />
</ul><br />
</div><br />
<br />
<br />
<a class="menuitem submenuheader" href="https://2011.igem.org/Team:Arizona_State/Lab">Lab</a><br />
<div class="submenu"><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Lab/Team">Team</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Lab/Photos">Photos</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Lab/Protocols">Protocols</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Lab/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Lab/Acknowledgements">Acknowledgements</a></li><br />
</ul><br />
</div><br />
<a class="menuitem submenuheader" href="https://2011.igem.org/Team:Arizona_State/Results">Results</a><br />
<div class="submenu"><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Results/Data">Data</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Parts">BioBricks</a></li><br />
</ul><br />
</div><br />
<!-- this page still exists but I don't think we'll get to it.<br />
<a class="menuitem" href="https://2011.igem.org/Team:Arizona_State/Modeling">Modeling</a><br />
--><br />
<a class="menuitem submenuheader" href="https://2011.igem.org/Team:Arizona_State/Lab">Notebook</a><br />
<div class="submenu"><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/AprilMay">April/May</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/June">June</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/July">July</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/August">August</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/September">September</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/PCRLog">Cas PCR Log</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/Sequences">Sequence Information</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Notebook/Other">Other Documents</a></li><br />
</ul><br />
</div><br />
<a class="menuitem submenuheader" href="https://2011.igem.org/Team:Arizona_State/Outreach">Human Practices</a><br />
<div class="submenu"><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Outreach/Events">Events</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Outreach/Collaborations">Collaborations</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Outreach/Exploring_Synthetic_Biology">Exploring Synthetic Biology</a></li><br />
<li><a href="https://2011.igem.org/Team:Arizona_State/Outreach/Outreach_in_Practice">Outreach in Practice</a></li><br />
</ul><br />
</div><br />
<br />
</body><br />
</html></div>
Hyder
http://2012.igem.org/Team:Arizona_State/Templates/main
Team:Arizona State/Templates/main
2012-05-09T05:23:05Z
<p>Hyder: Created page with "<html> <style type="text/css"> body {background: #FFFFFF;} .firstHeading { display: none; } #top-section { background-image: url('https://static.igem.org/mediawiki/2011/6/6d/ASU_bann..."</p>
<hr />
<div><html><br />
<style type="text/css"><br />
body {background: #FFFFFF;}<br />
.firstHeading { display: none; } <br />
#top-section {<br />
background-image: url('https://static.igem.org/mediawiki/2011/6/6d/ASU_banner_black.jpg');<br />
height:200px ! important;;<br />
}<br />
<br />
#p-logo {<br />
height:80px ! important;;<br />
overflow:hidden;<br />
visibility:hidden;<br />
}<br />
body<br />
{<br />
background-image:url('https://static.igem.org/mediawiki/2011/2/27/ASU_Circos_Ecoli.png');<br />
}<br />
</style><br />
</html><br />
<br><br />
<div style="font-size:18pt;"><br />
<font face="Calibri" style="color:#000000"><center>{{{title}}}<br />
----<br />
</center></font></div><br />
<div style="color: #ffffff; background-color: #ffffff; width: 900px"><br />
</div><br />
<br />
<div><br />
{| cellspacing="0"<br />
|-<br />
|style="background-color: #ffffff" width="75px" valign="top"|<br />
<br>{{:Team:Arizona State/Templates/sidebar2}}<br />
<br />
|width="880" valign="top" style="padding: 10px; border: 5px solid #ffffff; color: #000; background-color: white" | <br />
<div style="height: 450px; border: 1px solid #ffffff;"></div><div style="position: relative; margin-top: -450px; min-height: 450px">[[File:ASU_Logo.png|110px|right|link=Team:Arizona State]]<div>{{{content}}}</div><br />
</div><br />
|}<br />
----<br />
<center>{{:Team:Arizona State/Templates/footer}}</center></div>
Hyder
http://2012.igem.org/Team:Arizona_State
Team:Arizona State
2012-05-09T05:22:42Z
<p>Hyder: </p>
<hr />
<div>{{:Team:Arizona State/Templates/main|title=|content=<br />
<br />
We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition.<br />
<center>[[Image:ASU_tips.png|400px]]</center><br />
== Abstract ==<br />
<p>Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archaeal species. CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Our project focuses on developing a set of tools for synthetic control over the CRISPR pathway. This includes a method for creating polymers of repeat-spacer-repeat units, the development of CRISPR biobricks (CAS genes, leader sequences) for several CRISPR subtypes (E. coli, B. halodurans, and L. innocua), testing these components on plasmids containing GFP, and a software tool to collect and display CRISPR information, as well as select spacers from a particular sequence. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future.</p><br />
<br />
''[[Team:Arizona State/Project/Introduction|more]]''<br />
<br />
== What is CRISPR? ==<br />
<p>'''C'''lustered '''R'''egularly '''I'''nterspaced '''S'''hort '''P'''alindromic '''R'''epeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell.</p><br />
<center><br />
[[Image:ASU Crispr basic.png|600px|Basic mechanism]]<br><br />
A basic diagram of the CRISPR pathway. In this image, a CRISPR array is transcribed and used to locate a complementary sequence in the cell for degradation. '''[[Team:Arizona State/Project/CRISPR|More information]]'''.<br />
</center><br />
<br />
}}</div>
Hyder