User contributions
From 2012.igem.org
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- 03:57, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→gelextraction of digested pTUM104-TEF2-P (P806))
- 03:55, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Ligation of P572 (dig. pTUM104)with P684, P682, P683)
- 03:53, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of pTUM104 (3), P638 and psb1c3-TEF1-P)
- 03:50, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Picking of colonies from transformants with P403, P451, P450 and P51)
- 03:48, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Ligation of TEF2-P (clone P451) with pTUM104 and of P578 with P710)
- 03:47, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Nanodrop for concentration measuring of ADH-P,pTUM104,GFP)
- 03:45, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical gelelectrophoresis of pTUM104)
- 03:44, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion of pTUM104)
- 03:42, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Transformation EYFP (Dist. Kit) ECFP (Distr. Kit), Lig. pTUM104 1h, 50ng and 100 ng of E.coli Xl-1 blue)
- 03:40, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Self-ligation (self-circularization) of pTUM104)
- 03:38, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Transformation of overnight ligation with P265B (pTUM104))
- 03:36, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative gel of TEF1-P (P309), TEF2-P (P451) in psb1c3 and pTUM104 without GAL-P and f1)
- 03:32, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Ligation of pTUM104 without f1-origin and Gal-promoter)
- 03:30, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical gelelectrophoresis of pTUM104 undigested)
- 03:28, 27 September 2012 (diff | hist) N File:20120919 TEF2-pYESII+ luciferase (is a failure).png (top)
- 03:26, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Wednesday, August 15th)
- 03:24, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Miniprep of picked colonies (P806+ P791)=)
- 03:23, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→==Transformation of competent invitrogen YEAST cells with pTUM101-Luciferase, pTUM102-Luciferase, pTUM101-Limonensynth., pTUM102-Limonensynth., pTUM103-Limonensynth.)
- 03:22, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Transformation of E.coli with ligation product pTUM104 with PCR59, TEF2-P, TEF1-P and ADH1-P)
- 03:20, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Thursday, September 20th)
- 03:10, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Inoculation of yeast overnight culture with yeast transformated with pTUM101-P791 and pTUM103-P791)
- 03:09, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Saturday, September 22nd)
- 03:00, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of TEF1-T in psb1c3 and ADH1-T in psb1c3 with EcorI and SpeI)
- 02:57, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion of (P806+ P791))
- 02:46, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion and gelelectrophoresis of picked colonies)
- 02:44, 27 September 2012 (diff | hist) N File:20120918 ADH1-P und Limonens.png (top)
- 02:42, 27 September 2012 (diff | hist) N File:20120917 anal gel P827-P830.png (top)
- 02:40, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion and gelelectrophoresis of pTUM104-TEF1-P-Limonensynthases)
- 02:34, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion and gelelectrophoresis of picked colonies)
- 02:29, 27 September 2012 (diff | hist) N File:20120915 Pyes +TEF2P-Thaum, TEF2-P-Limonens.png (top)
- 02:27, 27 September 2012 (diff | hist) N File:20120915 Anal. Digest. pYES + TEF1P-Thaum, ADH1-P+Thaum.png (top)
- 02:25, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion and gelelctrophoresis of extracted DNA from transformants with TEF2-P-Thaum in pTUM104 , TEF1-P-Thaum, ADH1-P-Thaum)
- 02:06, 27 September 2012 (diff | hist) N File:20120914 Limonens (xbaI+pstI), und Tef1P in pYes2 und ADHP in pyes2.png (top)
- 02:04, 27 September 2012 (diff | hist) N File:20120913 20120913 Präp.Vedau PyesII-TUM-TEF2-P mit SpeI-HF und PSTI.png (top)
- 02:03, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of pTUM104-TEF2-P,pTUM104-TEF1-P, pTUM104-ADH1-P, Limonensynthases)
- 01:59, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Ligations)
- 01:55, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Sequencing of TEF1-T, TEF1-P, ADH1-P, ADH1-T)
- 01:54, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative Gelexlectrophoris and gelextraction of preparative digested probes)
- 01:50, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Nanodrop measurement)
- 01:48, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of BBa_E1010, BBa_J52008, pTUM104-TEF1, pTUM104-ADH1, P508 with EcorI-HF and SpeI-HF)
- 01:45, 27 September 2012 (diff | hist) N File:20120912 E1010,J52008 und Tef2-P-Limonens.png (top)
- 01:44, 27 September 2012 (diff | hist) N File:20120912 Psb1c3 (spe+EcorI), PyesII XbaI+PstI, TEF1-T (Spe+EcorI).png (top)
- 01:42, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of pTUM104 (3), P638 and psb1c3-TEF1-P)
- 01:36, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of BBa_J52008, BBa_E1010 and TEF2-pTUM104)
- 01:30, 27 September 2012 (diff | hist) N File:20120911 Anal. Verdau Reporterproteine.png (top)
- 01:29, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion of minipreped BBa_J52008, BBa_E2060, BBa_E1010, BBa_E0036)
- 01:27, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Nanodrop measurement)
- 01:23, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→analytical gelelectrophoresis with P678, P680 and P679)
- 01:23, 27 September 2012 (diff | hist) N File:20120906 TEF2-P (P450), ADH-P,TEF1-P in Pyes2tum.png (top)
- 01:21, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→analytical gelelectrophoresis with P678, P680 and P679)
- 01:18, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative gelelectrophoresis of ADH1-P, TEF1-P and TEF2-P in pTUM100)
- 01:17, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative gelelectrophoresis of ADH1-P, TEF1-P and TEF2-P in pTUM100)
- 01:16, 27 September 2012 (diff | hist) N File:20120906 ADH-P TEF1-P, TEF2-P in PyesII-TUM.png (top)
- 01:15, 27 September 2012 (diff | hist) N File:20120905 Präp ptumm100 mit 3 promotoren.png (top)
- 01:13, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative gelelectrophoresis of ADH1-P, TEF1-P and TEF2-P in pTUM100)
- 01:11, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Miniprep)
- 01:10, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative gelelectrophoresis of ADH1-P, TEF1-P and TEF2-P in pTUM104)
- 00:56, 27 September 2012 (diff | hist) N File:20120904 TEF1-P+Thaum, Cyc-T.png (top)
- 00:54, 27 September 2012 (diff | hist) N File:20120904 ADH1-P+ Thaumatin, TEF2-P und Thaum.png (top)
- 00:52, 27 September 2012 (diff | hist) N File:20120904 Promoteren ADH1,TEF2,TEF1 auf pyes2-tum.png (top)
- 00:51, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion of colonies with Cyc-T-TEF1-T+psb1c3 and Thaumatin with ADH1P-TEF2P-TEF1P in pTUM104)
- 00:47, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of Cyc1-T-Kol2, TEF1-P-Kol1, ADH1-P and TEF2-P Kol6 in psb1c3 with XbaI and PstI-HF)
- 00:44, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Nanodrop measurement)
- 00:42, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion of colonies with Cyc-T-TEF1-T+psb1c3 and Thaumatin with ADH1P-TEF2P-TEF1P in pTUM104)
- 00:39, 27 September 2012 (diff | hist) N File:20120903 TEF2-P+ Thaum + Negativ.png (top)
- 00:38, 27 September 2012 (diff | hist) N File:20120903 TEF1-T+Cyc-t in psb1c3.png (top)
- 00:36, 27 September 2012 (diff | hist) N File:20120903 Negativkontr.+ADH1 mit Thaum.png (top)
- 00:34, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Transformation of TEF1-T-psb1c3)
- 00:33, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Transformation of TEF1-T-psb1c3)
- 00:32, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Transformation of TEF1-T-psb1c3)
- 00:31, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparation of P588 (TEF2-P (450) for sequencing)
- 00:30, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical gelelectrophoresis of Cyc1-T, TEF1P, ADH1-P,TEF2-P and TEF1-T in psb1c3)
- 00:29, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion of colonies with Cyc-T-TEF1-T+psb1c3 and Thaumatin with ADH1P-TEF2P-TEF1P in pTUM104)
- 00:25, 27 September 2012 (diff | hist) N File:20120903 Cyc1-T in psb1c3 und Tef1-P+Thaum.png (top)
- 00:24, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical digestion of colonies with Cyc-T-TEF1-T+psb1c3 and Thaumatin with ADH1P-TEF2P-TEF1P in pTUM104)
- 00:21, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Picking of S. cerevisiae clones from SC-U plates (pTUM104_preprothaumatin and pTUM104_limonene-synthase) and YPD-Kan plates (integrational vector))
- 00:19, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Week 13)
- 00:13, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Nanodrop measurement)
- 00:12, 27 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Miniprepping of Clones (Schorsch! Nachtragen!))
- 23:49, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of TEF2-psb1c3 clones P588 and P591)
- 23:47, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative gelrun of P588 and P591)
- 23:45, 26 September 2012 (diff | hist) N File:20120830 TEF2-P ausgeschn mit xbaI und PstI.png (top)
- 23:44, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of TEF2-psb1c3 clones P588 and P591)
- 23:42, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Gelextraction of digested (Xba+PST-HF) ADH-P, GFP, Limonens. and psb1c3 (P577))
- 23:40, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Ligation of TEF1-T in psb1c3 P132)
- 23:39, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative gelrun of preparative digested ADH-P, Limonensynthase, GFP)
- 23:36, 26 September 2012 (diff | hist) N File:20120829 Gel 2 pYES2-tum.png (top)
- 23:34, 26 September 2012 (diff | hist) N File:20120829 Anal.png (top)
- 23:33, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical gelelectrophoresis of pTUM104)
- 23:29, 26 September 2012 (diff | hist) N File:20120829 Präp. Verdau ADH1-P (720bp), Limonensyn.png (top)
- 23:28, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Preparative digestion of GFP (P567), Limonesynthase (P509), ADH-P (720 bp) (P565))
- 23:26, 26 September 2012 (diff | hist) N File:20120828 pYes2-Tum Kol.png (top)
- 23:24, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical gelelectrophoresis of pTUM104 undigested)
- 23:19, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Miniprep)
- 23:16, 26 September 2012 (diff | hist) N File:20120828 pYES2-TUM Kol ungeschnitten (Gel1).png (top)
- 23:14, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Analytical gelelectrophoresis of pTUM104 undigested)
- 23:05, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→Nanodrop measurement of PCR 50-55 and P297-302)
- 23:01, 26 September 2012 (diff | hist) Team:TU Munich/Notebook/Labjournal (→PCR of ADH1-T, TEF2-P, TEF1-T from Saccharomyces cerv. genome)
- 22:56, 26 September 2012 (diff | hist) N File:20120810 Anal.Gel PCR TEF2p,TEF1t.png (top)
- 10:22, 26 September 2012 (diff | hist) File:Promoter strenghts .png (uploaded a new version of "File:Promoter strenghts .png": Reverted to version as of 07:19, 26 September 2012) (top)
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