Prokaryotic System/Secretion and Yeast Genome Integration

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Yeast System

Yeast Vectors 1

Yeast Vectors 2

A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae

Yeast Integrative Plasmid Vectors (YIp)

AL1 and GAL10 (galactose catabolism) selection

TRP1 selection

Prokaryotic System

Promoters

BBa-J23100 (strong)

BBa-J23108 (medium)

BBa-J23109 (weak)
Terminators

BBa-B0015
RBS

Constitutive

Secretion

HlyA Gene Sequence

A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium

Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain

Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins

Kumamolisin Info

57 kDa (beta-galactosidase: 540 kDa; [1] source)

1701 bp

567 aa

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