Prokaryotic System/Secretion and Yeast Genome Integration

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!align="center"|[[Team:Colorado_State|Home]]
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!align="center"|[[Team:Colorado_State/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Colorado_State Official Team Profile]
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!align="center"|[[Team:Colorado_State/Project|Project]]
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!align="center"|[[Team:Colorado_State/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Colorado_State/Modeling|Modeling]]
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!align="center"|[[Team:Colorado_State/Attributions|Attributions]]
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'''Yeast System''' <br/>
'''Yeast System''' <br/>
<li>[http://www.google.com/url?q=http%3A%2F%2Fbiochemie.web.med.uni-muenchen.de%2FYeast_Biol%2F04%2520Yeast%2520Molecular%2520Techniques.pdf&sa=D&sntz=1&usg=AFQjCNEnj2JbQh6nxUnja5PpZ3Vch5Jy6g Yeast Vectors 1]
<li>[http://www.google.com/url?q=http%3A%2F%2Fbiochemie.web.med.uni-muenchen.de%2FYeast_Biol%2F04%2520Yeast%2520Molecular%2520Techniques.pdf&sa=D&sntz=1&usg=AFQjCNEnj2JbQh6nxUnja5PpZ3Vch5Jy6g Yeast Vectors 1]

Latest revision as of 22:57, 27 June 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Sponsors Attributions


Yeast System

  • Yeast Vectors 1
  • Yeast Vectors 2
  • A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae

    Yeast Integrative Plasmid Vectors (YIp)
  • GAL1 and GAL10 (galactose catabolism) selection
  • TRP1 selection

    Prokaryotic System

    Promoters
  • BBa-J23100 (strong)
  • BBa-J23108 (medium)
  • BBa-J23109 (weak)
    Terminators
  • BBa-B0015
    RBS
  • Constitutive

    Secretion
  • HlyA Gene Sequence
  • A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium
  • Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain
  • Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins

    Kumamolisin Info
  • 57 kDa (beta-galactosidase: 540 kDa; source)
  • 1701 bp
  • 567 aa
    Retrieved from "http://2012.igem.org/Prokaryotic_System/Secretion_and_Yeast_Genome_Integration"