Prokaryotic System/Secretion and Yeast Genome Integration

From 2012.igem.org

(Difference between revisions)
(Created page with "'''Yeast System''' <br/> <li>[http://www.google.com/url?q=http%3A%2F%2Fbiochemie.web.med.uni-muenchen.de%2FYeast_Biol%2F04%2520Yeast%2520Molecular%2520Techniques.pdf&sa=D&sntz=1&...")
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'''Kumamolisin Info'''
'''Kumamolisin Info'''
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<li>57 kDa (beta-galactosidase: 540 kDa; [http://www.mpbio.com/detailed_info.php?family_key=02104939] source)
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<li>57 kDa (beta-galactosidase: 540 kDa; [http://www.mpbio.com/detailed_info.php?family_key=02104939 source])
<li>1701 bp
<li>1701 bp
<li>567 aa
<li>567 aa

Revision as of 17:38, 18 June 2012

Yeast System

  • Yeast Vectors 1
  • Yeast Vectors 2
  • A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae

    Yeast Integrative Plasmid Vectors (YIp)
  • AL1 and GAL10 (galactose catabolism) selection
  • TRP1 selection

    Prokaryotic System

    Promoters
  • BBa-J23100 (strong)
  • BBa-J23108 (medium)
  • BBa-J23109 (weak)
    Terminators
  • BBa-B0015
    RBS
  • Constitutive

    Secretion
  • HlyA Gene Sequence
  • A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium
  • Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain
  • Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins

    Kumamolisin Info
  • 57 kDa (beta-galactosidase: 540 kDa; source)
  • 1701 bp
  • 567 aa
    Retrieved from "http://2012.igem.org/Prokaryotic_System/Secretion_and_Yeast_Genome_Integration"