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Notebook ethan
From 2012.igem.org
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Revision as of 22:38, 20 July 2012
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Yeast Integrative Plasmid Vectors (YIp)
Promoters
Terminators
RBS
Secretion
Kumamolisin Info
Ethan's Research: Yeast Genome Integration and Bacterial Secretion of Kumamolisin
Yeast System
- Yeast Vectors 1
- Yeast Vectors 2
- A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae
Yeast Integrative Plasmid Vectors (YIp)
Prokaryotic System
Promoters
Terminators
RBS
Secretion
- HlyA Gene Sequence
- A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium
- Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain
- Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins
Kumamolisin Info
- 57 kDa (beta-galactosidase: 540 kDa; source)
- 1701 bp
- 567 aa
