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[[Team:Columbia-Cooper-NYC/Future_Directions|Future Directions]]
[[Team:Columbia-Cooper-NYC/Future_Directions|Future Directions]]
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<li><a class="head" href="#"> Introduction</a>
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<li><a href="/Team:Cambridge">Home</a></li>
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<li><a href="/Team:Cambridge/TheTeam">Meet the Team</a></li>
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<li><a href="/Team:Cambridge/Photos">Photo Gallery</a></li>
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<li><a href="/Team:Cambridge/Videos">Videos</a></li>
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<li><a href="/Team:Cambridge/BioBricks">Parts submitted</a></li>
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</ul>
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</li>
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<li><a class="head" href="#">Project Firefly</a>
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<ul class="llmenu llmenugreen">
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<li><a href="/Team:Cambridge/Bioluminescence">Introduction</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Background_Firefly">Background</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Colour">Coloured outputs</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Luciferin_Regeneration">Luciferin recovery</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Firefly_Modelling">Modelling</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Firefly_Characterisation">Characterisation</a></li>
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</ul>
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</li>
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<li><a class="head" href="#">Project Vibrio</a>
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<ul class="llmenu llmenublue">
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<li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">Introduction</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Background">Background</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/G28">The LuxBrick</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Codon_optimisation">Codon optimisation</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Modelling">Modelling</a></li>
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<li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Characterisation">Characterisation</a></li>
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</ul>
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</li>
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<li><a class="head" href="#">Tools</a>
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<ul class="llmenu llmenured">
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<li><a href="/Team:Cambridge/Tools/Gibson">Gibthon</a></li>
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<li><a href="/Team:Cambridge/Tools/GenBank">BioBrick → GenBank</a></li>
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<li><a href="/Team:Cambridge/Tools/Ligate">Ligation Calculator</a></li>
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<li><a href="/Team:Cambridge/Tools/Eglometer">The E.glometer</a></li>
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</ul>
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</li>
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<li><a class="head" href="#">Gibson Assembly</a>
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<ul class="llmenu llmenublue">
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<li><a href="/Team:Cambridge/Gibson/Introduction">Introduction</a></li>
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<li><a href="/Team:Cambridge/Gibson/Mechanism">How it works</a></li>
 +
<li><a href="/Team:Cambridge/Gibson/Protocol">Protocol</a></li>
 +
<li><a href="/Team:Cambridge/Gibson/RFC">RFC</a></li>
 +
</ul>
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</li>
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 +
<li><a class="head" href="#">Considerations</a>
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<ul class="llmenu llmenublue">
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<li><a href="/Team:Cambridge/Human_Practices">Human Practices</a></li>
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<li><a href="/Team:Cambridge/Safety">Safety</a></li>
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</ul>
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</li>
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 +
<li><a class="head" href="#">Future applications</a>
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<ul class="llmenu llmenured">
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<li><a href="/Team:Cambridge/Tools/microMeasure">Biosensors</a></li>
 +
<li><a href="/Team:Cambridge/Tools/Lighting">Lighting</a></li>
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</ul>
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</li>
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<li><a class="head" href="/Team:Cambridge/Notebook/Week1">Notebook</a>
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<ul class="llmenu llmenuyellow">
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<li><a href="/Team:Cambridge/Notebook/Summary">Summary</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week1">Week 1</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week2">Week 2</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week3">Week 3</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week4">Week 4</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week5">Week 5</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week6">Week 6</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week7">Week 7</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/Week8">Week 8</a></li>
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<li><a href="/Team:Cambridge/Notebook/Week9">Week 9</a></li>
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<li><a href="/Team:Cambridge/Notebook/Week10">Week 10</a></li>
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<li><a href="/Team:Cambridge/Notebook/Week11">Week 11</a></li>
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<li><a href="/Team:Cambridge/Notebook/Week12">Week 12</a></li>
 +
<li><a href="/Team:Cambridge/Notebook/FurtherWork">Beyond Week 12</a></li>
 +
 +
<li><a href="/Team:Cambridge/Protocols">Protocols</a> </li>
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<li><a href="/Team:Cambridge/Partners" class="head">Our Partners</a>
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</div>
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<div id="lefttop" style="background:#96d446; background-image:url('http://2010.igem.org/wiki/images/a/ad/Cam-Gradient-Overlay.png');" class="secheader">Abstract</div>
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<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> We placed genes from <a style="color:#6bbe00 !important" href="http://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">bacteria</a> into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include  and quantitative <a style="color:#6bbe00 !important" href="http://2010.igem.org/Team:Cambridge/Tools/microMeasure">biosensors</a> and biological <a style="color:#6bbe00 !important" href="http://2010.igem.org/Team:Cambridge/Tools/Lighting">alternatives</a> to conventional lighting.</p>
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<div id="cbox" style="float:left; height:200px;  width:241px; margin-left:5px;" class="tophome">
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<div id="ctop" style="background:#fad72a;background-image:url('http://2010.igem.org/wiki/images/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Multimedia</div>
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<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see view videos of our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a> and <a style="color:#d9b400 !important" onclick="displayGibson('http://www.cambridgeigem.org/video.php'); return false;" href="http://www.cambridgeigem.org/video.php?nojs=true">project overview</a>.
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<script>
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<div id="rightbox" style="float:left;  height:200px; margin-left:5px; width:242px;" class="tophome">
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<div id="rtop" style="background:#fb5c2b;background-image:url('http://2010.igem.org/wiki/images/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Tools</div>
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<div id="rcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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<div style="position:relative; top:10px;">
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<a href="http://www.gibthon.org" id="leftbox" style="background:url('http://2010.igem.org/wiki/images/5/53/Cambridge-Gibthon.png'); position:absolute; width:120px; height:100px; left:0;top:0; background-repeat:no-repeat; display:block;text-align:center; padding-top:70px; background-position: center top; color:#e53500 !important;" >
 +
Gibson<br />Assembly
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</a>
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<a href="http://www.gibthon.org/ligate.html" id="rightbox" style="background:url('http://2010.igem.org/wiki/images/b/b1/Cambridge-Flask.png'); position:absolute; width:120px; height:100px; left:120px; text-align:center;top:0; background-repeat:no-repeat;display:block; padding-top:70px; background-position: center top; color:#e53500 !important;" />
 +
Ligation<br />Calculator
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</a>
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</div>
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</div>
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</div>
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</p>
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<p>&nbsp;</p>
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<p>
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<div style="width:730px; background-color:#386abc;" class="secheader">The Project</div>
 +
<div class="contwrap">
 +
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<img src="http://2010.igem.org/wiki/images/7/70/Front.jpg" style="margin-left:4px; margin-bottom:5px; width:710px; margin-top:-2px;">
 +
<div style="height:30px; position:relative; font-size:12px; color:#6e6e6e; line-height:15px;">
 +
<div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div>
 +
<div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div>
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<div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div>
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</div>
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</p><p><br />
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Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for <a href="http://2010.igem.org/Team:Cambridge/Tools/microMeasure" class="external text" title="http://2010.igem.org/Team:Cambridge/Tools/microMeasure" rel="nofollow"><b>biosensors</b></a> and as <a href="http://2010.igem.org/Team:Cambridge/Tools/Lighting" class="external text" title="http://2010.igem.org/Team:Cambridge/Tools/Lighting" rel="nofollow"><b>natural light sources</b></a>. We also developed software tools to aid construction of BioBrick parts and devices.
 +
</p><p><h2 class="noline" style="margin-top:30px;">Project Firefly</h2>
 +
</p><p>We adopted a number of strategies to extend the use of <b>firefly luciferase</b>:
 +
</p>
 +
<ul><li> <a href="http://2010.igem.org/Team:Cambridge/Codons" class="external text" title="http://2010.igem.org/Team:Cambridge/Codons" rel="nofollow"><b>Codon optimisation</b></a> for increased light output
 +
</li><li> Use of a <a href="http://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration" class="external text" title="http://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration" rel="nofollow"><b>luciferin regenerating enzyme</b></a>.
 +
</li><li> Mutagenesis to create a number of <a href="http://2010.igem.org/Team:Cambridge/Bioluminescence/Colour" class="external text" title="http://2010.igem.org/Team:Cambridge/Bioluminescence/Colour" rel="nofollow"><b>different colours</b></a>
 +
</li></ul>
 +
<p><h2 class="noline" style="margin-top:30px;">Project Vibrio</h2>
 +
We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from <b>Vibrio fischeri</b>.  We believe we have created the <a href="http://2010.igem.org/Team:Cambridge/Bioluminescence/G28" class="external text" title="http://2010.igem.org/Team:Cambridge/Bioluminescence/G28" rel="nofollow"><b>first BioBrick</b></a> to emit light in normal E. coli strains without the addition of any external substrate.
 +
</p><p><h2 class="noline" style="margin-top:30px;">Tools</h2>
 +
</p><p>During our project we made extensive use of <a href="http://2010.igem.org/Team:Cambridge/Gibson/Introduction" class="external text" title="http://2010.igem.org/Team:Cambridge/Gibson/Introduction" rel="nofollow"><b>Gibson Assembly</b></a> to manufacture our parts, and have submitted an <a href="http://2010.igem.org/Team:Cambridge/Gibson/RFC" class="external text" title="http://2010.igem.org/Team:Cambridge/Gibson/RFC" rel="nofollow"><b>RFC</b></a> to the <a href="http://bbf.openwetware.org/" class="external text" title="http://bbf.openwetware.org/" rel="nofollow"><b>BioBricks Foundation</b></a> to help future teams make best use of this technique.
 +
</p><p>Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
 +
</p>
 +
<ul><li> <a href="http://2010.igem.org/Team:Cambridge/Tools/Gibson" class="external text" title="http://2010.igem.org/Team:Cambridge/Tools/Gibson" rel="nofollow"><b>Gibthon Construct Designer</b></a> allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for <a href="http://2010.igem.org/Team:Cambridge/Gibson/Introduction" class="external text" title="http://2010.igem.org/Team:Cambridge/Gibson/Introduction" rel="nofollow"><b>Gibson Assembly</b></a>.
 +
</li><li> <a href="http://2010.igem.org/Team:Cambridge/Tools/GenBank" class="external text" title="http://2010.igem.org/Team:Cambridge/Tools/GenBank" rel="nofollow"><b>BioBrick → GenBank</b></a> allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
 +
</li><li> The <a href="http://2010.igem.org/Team:Cambridge/Tools/Ligate" class="external text" title="http://2010.igem.org/Team:Cambridge/Tools/Ligate" rel="nofollow"><b>Ligation Calculator</b></a> is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
 +
</li><li> The <a href="http://2010.igem.org/Team:Cambridge/Tools/Eglometer" class="external text" title="http://2010.igem.org/Team:Cambridge/Tools/Eglometer" rel="nofollow"><b>E.glometer</b></a> is a cheap, easily built, piece of electronics for measuring bioluminescence.  It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in <a href="http://2010.igem.org/Team:Cambridge/Tools/microMeasure" class="external text" title="http://2010.igem.org/Team:Cambridge/Tools/microMeasure" rel="nofollow"><b>quantitative biosensors</b></a>.
 +
</li></ul>
 +
<p><h2 class="noline" style="margin-top:30px;">Achievements in iGEM competition</h2>
 +
</p>
 +
<ul><li> Finalist
 +
</li><li> Winner of "Best Wiki" award (awarded jointly to Cambridge and <a href="http://2010.igem.org/Team:Imperial_College_London" class="external text" title="http://2010.igem.org/Team:Imperial_College_London" rel="nofollow"><b>Imperial College London</b></a>)
 +
</li><li> Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
 +
</li><li> Awarded a Gold Medal
 +
</li></ul>
 +
<p>Please see <a href="http://http://igem.org/Results" class="external text" title="http://http://igem.org/Results" rel="nofollow"><b>iGEM official results page</b></a> to see how all the teams did.
 +
</p><p><h2 class="noline" style="margin-top:30px;">In the news</h2>
 +
</p>
 +
<ul><li> <a href="http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html" class="external text" title="http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html" rel="nofollow">New Scientist article</a>
 +
</li><li> <a href="http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html" class="external text" title="http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html" rel="nofollow">Daily Mail article</a>
 +
</li><li> <a href="http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html" class="external text" title="http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html" rel="nofollow">The Telegraph Article</a>
 +
</li></ul>
 +
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:702px;"><a href="/Image:Cambridge_team_pictwo2010.jpg" class="image" title="The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins"><img alt="The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins" src="/wiki/images/b/b0/Cambridge_team_pictwo2010.jpg" width="700" height="440" border="0" class="thumbimage" /></a>  <div class="thumbcaption">The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins</div></div></div></div>
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Revision as of 20:20, 15 September 2012

References


Parts Submitted


Outreach


Collaborations


Security


Acknowledgements


Overview


University Profiles


Data and Conclusions


Future Directions






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Team:Cambridge

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