Gluten digestion enzyme plasmid.

From 2012.igem.org

(Difference between revisions)
Line 24: Line 24:
http://www.blackwellpublishing.com/genecloning/pdfs/chapter7.pdf (7.1.3)
http://www.blackwellpublishing.com/genecloning/pdfs/chapter7.pdf (7.1.3)
https://static.igem.org/mediawiki/2008/4/4e/JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf
https://static.igem.org/mediawiki/2008/4/4e/JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf
 +
 +
Vectors derived from the 2mm plasmid are called yeast episomal plasmids
 +
(YEps). Some YEps contain the entire 2mm plasmid, others include just the
 +
2mm origin of replication. An example of the latter type is YEp13 (Figure 7.3).
 +
YEp13 illustrates several general features of yeast cloning vectors. First, it
 +
is a shuttle vector. As well as the 2mm origin of replication and the selectable
 +
LEU2 gene, YEp13 also includes the entire pBR322 sequence, and can therefore
 +
replicate and be selected for in both yeast and E. coli. There are several
 +
lines of reasoning behind the use of shuttle vectors. One is that it may be difficult
 +
to recover the recombinant DNA molecule from a transformed yeast
 +
colony. This is not such a problem with YEps, which are present in yeast cells
 +
primarily as plasmids, but with other yeast vectors, which may integrate into

Revision as of 17:01, 8 June 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

This page is for Guy's research.

Stable/Genomic encoding

http://partsregistry.org/wiki/index.php?title=Part:BBa_K590023

I am currently viewing Yeast integrative plasmid,(YIp), whhich are yeast vectors that utilize integration into the yeast chromosome for survival and are used when studying a DNA gene. http://en.wikipedia.org/wiki/Yeast_artificial_chromosome

http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.37.100183.001345 http://www.pnas.org/content/75/4/1929.full.pdf http://nar.oxfordjournals.org/content/29/12/e59.full http://www.blackwellpublishing.com/genecloning/pdfs/chapter7.pdf (7.1.3) https://static.igem.org/mediawiki/2008/4/4e/JHU_0708_paper_Shuttle_Vectors_with_Multiple_Unique_Restriction_Sites.pdf

Vectors derived from the 2mm plasmid are called yeast episomal plasmids (YEps). Some YEps contain the entire 2mm plasmid, others include just the 2mm origin of replication. An example of the latter type is YEp13 (Figure 7.3). YEp13 illustrates several general features of yeast cloning vectors. First, it is a shuttle vector. As well as the 2mm origin of replication and the selectable LEU2 gene, YEp13 also includes the entire pBR322 sequence, and can therefore replicate and be selected for in both yeast and E. coli. There are several lines of reasoning behind the use of shuttle vectors. One is that it may be difficult to recover the recombinant DNA molecule from a transformed yeast colony. This is not such a problem with YEps, which are present in yeast cells primarily as plasmids, but with other yeast vectors, which may integrate into